Publications by authors named "Haiyan Li"

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Detection and validation of cis-regulatory motifs in osmotic stress-inducible synthetic gene switches via computational and experimental approaches.

Funct Plant Biol 2022 Aug 9. Epub 2022 Aug 9.

Synthetic cis-regulatory modules can improve our understanding of gene regulatory networks. We applied an ensemble approach for de novo cis motif discovery among the promoters of 181 drought inducible differentially expressed soybean (Glycine max L.) genes. A total of 43 cis motifs were identified in promoter regions of all gene sets using the binding site estimation suite of tools (BEST). Comparative analysis of these motifs revealed similarities with known cis-elements found in PLACE database and led to the discovery of cis-regulatory motifs that were not yet implicated in drought response. Compiled with the proposed synthetic promoter design rationale, three synthetic assemblies were constructed by concatenating multiple copies of drought-inducible cis motifs in a specific order with inter-motif spacing using random bases and placed upstream of 35s minimal core promoter. Each synthetic module substituted 35S promoter in pBI121 and pCAMBIA3301 to drive glucuronidase expression in soybean hairy roots and Arabidopsis thaliana L. Chimeric soybean seedlings and 3-week-old transgenic Arabidopsis plants were treated with simulated with different levels of osmotic stress. Histochemical staining of transgenic soybean hairy roots and Arabidopsis displayed drought-inducible GUS activity of synthetic promoters. Fluorometric assay and expression analysis revealed that SP2 is the better manual combination of cis-elements for stress-inducible expression. qRT-PCR results further demonstrated that designed synthetic promoters are not tissue-specific and thus active in different parts upon treatment with osmotic stress in Arabidopsis plants. This study provides tools for transcriptional upgradation of valuable crops against drought stress and adds to the current knowledge of synthetic biology.
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http://dx.doi.org/10.1071/FP21314DOI Listing
August 2022

Inositol hexakisphosphate kinases differentially regulate trafficking of vesicular glutamate transporters 1 and 2.

Front Cell Neurosci 2022 22;16:926794. Epub 2022 Jul 22.

Department of Psychiatry, School of Medicine, University of California San Francisco, San Francisco, CA, United States.

Inositol pyrophosphates have been implicated in cellular signaling and membrane trafficking, including synaptic vesicle (SV) recycling. Inositol hexakisphosphate kinases (IP6Ks) and their product, diphosphoinositol pentakisphosphate (PP-IP or IP7), directly and indirectly regulate proteins important in vesicle recycling by the activity-dependent bulk endocytosis pathway (ADBE). In the present study, we show that two isoforms, IP6K1 and IP6K3, are expressed in axons. The role of the kinases in SV recycling are investigated using pharmacologic inhibition, shRNA knockdown, and and knockout mice. Live-cell imaging experiments use optical reporters of SV recycling based on vesicular glutamate transporter isoforms, VGLUT1- and VGLUT2-pHluorins (pH), which recycle differently. VGLUT1-pH recycles by classical AP-2 dependent endocytosis under moderate stimulation conditions, while VGLUT2-pH recycles using AP-1 and AP-3 adaptor proteins as well. Using a short stimulus to release the readily releasable pool (RRP), we show that IP6K1 KO increases exocytosis of both VGLUT1-and VGLUT2-pH, while IP6K3 KO decreases the amount of both transporters in the RRP. In electrophysiological experiments we measure glutamate signaling with short stimuli and under the intense stimulation conditions that trigger bulk endocytosis. IP6K1 KO increases synaptic facilitation and IP6K3 KO decreases facilitation compared to wild type in CA1 hippocampal Schaffer collateral synapses. After intense stimulation, the rate of endocytosis of VGLUT2-pH, but not VGLUT1-pH, is increased by knockout, knockdown, and pharmacologic inhibition of IP6Ks. Thus IP6Ks differentially affect the endocytosis of two SV protein cargos that use different endocytic pathways. However, while IP6K1 KO and IP6K3 KO exert similar effects on endocytosis after stimulation, the isoforms exert different effects on exocytosis earlier in the stimulus and on the early phase of glutamate release. Taken together, the data indicate a role for IP6Ks both in exocytosis early in the stimulation period and in endocytosis, particularly under conditions that may utilize AP-1/3 adaptors.
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http://dx.doi.org/10.3389/fncel.2022.926794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355605PMC
July 2022

Long Non-Coding RNA LINC00052 Targets miR-548p/Notch2/Pyk2 to Modulate Tumor Budding and Metastasis of Human Breast Cancer.

Biochem Genet 2022 Aug 2. Epub 2022 Aug 2.

Department of Breast Surgery, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital of Sun Yat-Sen University, No. 26 Erheng Road, Yuancun, Tianhe District, Guangzhou, 510655, Guangdong, China.

Abnormal expression of long non-coding RNAs (lncRNAs) is involved in many pathological processes of cancers. However, the role of lncRNA LINC00052 in breast cancer progression is still unclear. Here, LINC00052 expression was detected by in situ hybridization and quantitative real-time PCR assays. Cell Counting Kit-8, wound healing, and transwell assays were used to investigate changes in the proliferation, migration, and invasion of breast cancer cells. MiR-548p was found associated with LINC00052 or Notch2 by RNA pull-down, dual-luciferase reporter, and qRT-PCR assays. The effect of LINC00052 on lung metastasis was explored through in vivo experiments. High LINC00052 expression was observed in breast cancer tissues and cells. LINC00052 silencing inhibited the proliferation, migration, and invasion of MCF7 cells, and LINC00052 overexpression produced the opposite results. MiR-548p, a target gene of LINC00052, partially rescued the effects of LINC00052 on proliferation, migration, and invasion of MCF7. Notch2 was the target of miR-548p and LINC00052 could promote Notch2 expression. Moreover, the phosphorylation of proline-rich tyrosine kinase 2 (Pyk2), a downstream factor of Notch2, was increased by LINC00052, and a Pyk2 mutant could inhibit the cell migration and invasion induced by LINC00052 overexpression in MDA-MB-468 cells, which was similar to the function of the miR-548p mimic. We further demonstrated that LINC00052 exacerbated the metastases of breast cancer cells in vivo. Our research demonstrated that LINC00052 is highly expressed in breast cancer and promotes breast cancer proliferation, migration, and invasion via the miR-548p/Notch2/Pyk2 axis. LINC00052 could serve as a potential therapeutic target for breast cancer.
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http://dx.doi.org/10.1007/s10528-022-10255-yDOI Listing
August 2022

Carbonylative Cross-Coupling Reaction of Allylic Alcohols and Organoalanes with 1 atm CO Enabled by Nickel Catalysis.

Angew Chem Int Ed Engl 2022 Jul 27. Epub 2022 Jul 27.

East China University of Science and Technology, School of Chemistry and Molecular Engineering, 130 Meilong Road, 200237, Shanghai, CHINA.

A nickel-catalyzed three-component carbonylative crosscoupling reaction of allylic alcohols and organoalanes with CO at atmospheric pressure is reported, enabling the expedient formation of β,γ-unsaturated ketones with broad scope. Particularly, the chemoselective allylic carbonylation of diols further highlights the practicability of this protocol. The leverage of organoalanes as both the coupling components and the activators for the alcohol functionalization is crucial for this method, thus no extraneous activators are required.
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http://dx.doi.org/10.1002/anie.202210484DOI Listing
July 2022

CR-GCN: Channel-Relationships-Based Graph Convolutional Network for EEG Emotion Recognition.

Brain Sci 2022 Jul 26;12(8). Epub 2022 Jul 26.

School of Computer Science and Technology, Kashi University, Kashi 844008, China.

Electroencephalography (EEG) is recorded by electrodes from different areas of the brain and is commonly used to measure neuronal activity. EEG-based methods have been widely used for emotion recognition recently. However, most current methods for EEG-based emotion recognition do not fully exploit the relationship of EEG channels, which affects the precision of emotion recognition. To address the issue, in this paper, we propose a novel method for EEG-based emotion recognition called CR-GCN: Channel-Relationships-based Graph Convolutional Network. Specifically, topological structure of EEG channels is distance-based and tends to capture local relationships, and brain functional connectivity tends to capture global relationships among EEG channels. Therefore, in this paper, we construct EEG channel relationships using an adjacency matrix in graph convolutional network where the adjacency matrix captures both local and global relationships among different EEG channels. Extensive experiments demonstrate that CR-GCN method significantly outperforms the state-of-the-art methods. In subject-dependent experiments, the average classification accuracies of 94.69% and 93.95% are achieved for valence and arousal. In subject-independent experiments, the average classification accuracies of 94.78% and 93.46% are obtained for valence and arousal.
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http://dx.doi.org/10.3390/brainsci12080987DOI Listing
July 2022

Domain Adaptation with Data Uncertainty Measure Based on Evidence Theory.

Entropy (Basel) 2022 Jul 13;24(7). Epub 2022 Jul 13.

School of Computer Science and Technology, Kashi University, Kashi 844006, China.

Domain adaptation aims to learn a classifier for a target domain task by using related labeled data from the source domain. Because source domain data and target domain task may be mismatched, there is an uncertainty of source domain data with respect to the target domain task. Ignoring the uncertainty may lead to models with unreliable and suboptimal classification results for the target domain task. However, most previous works focus on reducing the gap in data distribution between the source and target domains. They do not consider the uncertainty of source domain data about the target domain task and cannot apply the uncertainty to learn an adaptive classifier. Aimed at this problem, we revisit the domain adaptation from source domain data uncertainty based on evidence theory and thereby devise an adaptive classifier with the uncertainty measure. Based on evidence theory, we first design an evidence net to estimate the uncertainty of source domain data about the target domain task. Second, we design a general loss function with the uncertainty measure for the adaptive classifier and extend the loss function to support vector machine. Finally, numerical experiments on simulation datasets and real-world applications are given to comprehensively demonstrate the effectiveness of the adaptive classifier with the uncertainty measure.
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http://dx.doi.org/10.3390/e24070966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317131PMC
July 2022

Identification and quantification of common microplastics in table salts by a multi-technique-based analytical method.

Anal Bioanal Chem 2022 Jul 20. Epub 2022 Jul 20.

National Centre for Food Science, Singapore Food Agency, 10 Perahu Road, Singapore, 718837, Singapore.

Microplastics (MPs) are considered as contaminants of emerging concern to the environment and our food chains in recent years. In this study, we presented a multi-technique-based analytical method for detection of MPs through a combination of microscope-FTIR (μ-FTIR) with pyrolysis-GC/MS (Py-GC/MS) to achieve 3-dimensional (3D) information for the identification of polymer type, characterization of particle size and morphology, and quantification of MPs based on both particle number and mass of plastics. Plastics that are commonly used and disposed of, including polyethylene (PE), polypropylene (PP), polystyrene (PS), polyethylene terephthalate (PET), poly vinyl chloride (PVC), polyamide (PA), and poly(methyl methacrylate) (PMMA), were covered in this study. Sample extraction and separation procedures were optimized for these microplastics in table salts where good recoveries (> 75%) were achieved. To further enhance the detection sensitivity in simultaneous quantification of multiple polymers in a sample, a serial dissolution approach with different solvents was developed for the detection of all 7 types of plastics. The established sample preparation process and multi-technique-based analytical method were validated with polymer standards in table salts, resulting in satisfactory qualification and quantification for all samples tested. A retail survey of MPs in table salts was conducted with the developed analytical method, revealing that MPs were present in all commercially available table salts. The total number of MP particles varied from 20 to 125 particles/kg and the total mass contents of seven types of plastics ranged from 30 to 530 µg/kg in table salts.
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http://dx.doi.org/10.1007/s00216-022-04226-wDOI Listing
July 2022

Synthesis of Well-Ordered Mesoporous Aluminosilicates with High Aluminum Contents: The Challenge and the Promise.

Inorg Chem 2022 Aug 15;61(30):11820-11829. Epub 2022 Jul 15.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.

SBA-15 has recently emerged as a potential material for the catalytic conversion of large molecules. Usually, SBA-15 has a low content of aluminum due to the conventional acidic synthesis medium. Although a few approaches have been adopted to prepare Al-SBA-15 with a high alumina content, it is still challenging to prepare well-ordered Al-SBA-15 with a high alumina content. Here, we demonstrate a facile synthesis process in neutral mediums for the grafting of Al into the framework of SBA-15. This approach relies mainly on the dissociation of Si-O-Si bonds and the polymerization of Si-O-Si/Al bonds promoted by sodium persulfate (SPS) in neutral mediums. In this way, well-ordered AlSBA-15 with a high aluminum content and enhanced acidity was obtained. Results of X-ray fluorescence spectroscopy (XRF) showed an (SiO)/(AlO) ratio of 13.7, much lower than that of the conventional sample (21.7) obtained in acidic medium. The characterization results indicated the presence of a well-ordered Al-containing mesophase with high hydrothermal stability. Notably, the Al content and the acidity of AlSBA-15 can be tuned by changing the SPS amount.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01571DOI Listing
August 2022

The Effect of Azithromycin Combined with Palm Massage on Pulmonary Function of Children with Mycoplasma Pneumonia.

Authors:
Haiyan Li Dandan Xu

Evid Based Complement Alternat Med 2022 4;2022:1857055. Epub 2022 Jul 4.

Department of Respiratory, Maternal and Child Health Hospital of Jiujiang City, Jiujiang, Jiangxi, China.

Objective: The study aimed to determine the effect of azithromycin combined with palm massage on the pulmonary function of children suffering from mycoplasma pneumonia, as well as associated complications.

Methods: A total of 60 cases of children suffering from mycoplasma pneumonia admitted to our hospital from May 2018 to May 2020 were selected as study subjects and assigned to either the observation group or the control group. Lung function indices and changes in the levels of inflammatory markers were measured before and after treatment in both groups, and the results were compared to assess treatment efficacy.

Results: There was no statistically significant difference in lung function indices between the two groups before treatment ( > 0.05), while there was a statistically significant difference between the two groups after treatment ( < 0.05), with the observation group outperforming the control group. Similarly, there was no statistically significant difference in the levels of such inflammatory markers as IL-6 and TNF- between the two groups before treatment ( > 0.05). After treatment, there was a decrease in the levels of inflammatory markers in both groups, but a much larger decrease was seen in the observation group ( < 0.05) than in the control group. The total treatment efficacy in the observation group was 93.3%, which was significantly higher than that of 70.0% in the control group ( < 0.05).

Conclusion: The application of palm massage combined with azithromycin in the treatment of children suffering from mycoplasma pneumonia produces outstanding results in terms of relieving symptoms and improving pulmonary function.
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http://dx.doi.org/10.1155/2022/1857055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273380PMC
July 2022

Reduction effect of oral pravastatin on the acute phase response to intravenous zoledronic acid: protocol for a real-world prospective, placebo-controlled trial.

BMJ Open 2022 07 13;12(7):e060703. Epub 2022 Jul 13.

Department of Orthopaedics, Peking University Third Hospital, Beijing, China

Introduction: Zoledronic acid (ZA) has been used as a first-line treatment in patients with osteoporosis (OP) who receive an annual injection of 5 mg. However, side effects of bone pain and fever, known as the acute phase response (APR), have often been observed after clinical usage. A meta-analysis reported that the incidence of APR was 49.4% among patients with OP who received ZA for the first time and that 30% of patients with these adverse effects refused treatment in the following year. As a clinically used hypolipidaemic drug, statins can inhibit 3-hydroxy-3-methylglutaryl coenzyme A reductase to block the pathway upstream of farnesyl pyrophosphate synthase. This process can decrease the accumulation of isopentenyl pyrophosphate to prevent γδT-cell activation and inflammatory factor production, blocking APR occurrence. The aim of this study is to determine the reduction effect of oral pravastatin on APR and investigate the possible mechanisms underlying the effect in vivo.

Methods And Analysis: This will be a single-centre, placebo-controlled trial. Female participants will be allocated at a 1:1 ratio to receive either oral pravastatin or a placebo at 1-hour predose and 24 and 48 hours post-administration of ZA. The primary outcome will be the incidence of APR within 72 hours after ZA infusion. The secondary outcomes will include the occurrence time and severity of APR and the frequency and amount of acetaminophen usage within 72 hours after ZA infusion. This study will determine the preventive effect of oral pravastatin on APR in Chinese patients with OP, supporting the clinical application of ZA to alleviate concerns regarding safety and increase patient compliance.

Ethics And Dissemination: This study protocol has been registered with ClinicalTrials.gov. This study protocol was reviewed and approved by the Peking University Third Hospital Medical Science Research Ethics Committee. The results will be published in scientific peer-reviewed journals.

Trial Registration Number: NCT04719481.
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http://dx.doi.org/10.1136/bmjopen-2021-060703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280907PMC
July 2022

Giant phyllodes tumor of the breast: A case report.

Asian J Surg 2022 Jul 6. Epub 2022 Jul 6.

Department of Breast Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital of Sun Yat-sen University, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.094DOI Listing
July 2022

Exploring Spatial Relationship between Restoration Suitability and Rivers for Sustainable Wetland Utilization.

Int J Environ Res Public Health 2022 Jul 1;19(13). Epub 2022 Jul 1.

National and Local Joint Laboratory of Wetland and Ecological Conservation, Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, Harbin 150040, China.

Wetlands are important ecosystems for biodiversity preservation and environmental regulation. However, the integrity of wetland ecosystems has been seriously compromised and damaged due to the reckless and indiscriminate exploitation of wetland resources during economic development by human society. Hence, wetland restoration has now attracted wide attention. Understanding wetland restoration suitability and its relationship with river grade and river distance is an important step in further implementing wetland restoration and ensuring an orderly wetland development and utilization. In this study, wetland restoration suitability is evaluated combining natural and human factors. Taking its result as an important basis, the spatial distribution characteristics of different levels of wetland restoration suitability are discussed for the studied region; the percentage distribution of different levels of wetland restoration suitability is analyzed for 10 km long buffer zones of rivers of different grades, and the association between the distribution of different levels of wetland restoration suitability and the river distance (2, 4, 6, 8, and 10 km) is also analyzed for different buffer zones of rivers in different grades. Our findings show that the spatial distribution of wetland restoration suitability is closely associated with the grade of rivers and the distance of the wetland patches from the river. The higher the river grade, the higher the percentage of the wetland with high restoration suitability within the same river distance. The percentage of wetlands with high restoration suitability has shown a notably decreasing trend as the river distance increases for the areas beside rivers of all grades, while the percentage of a wetland area with relatively high restoration suitability tends to increase as the river distance increases for the areas beside rivers of grade I and II and does not have a noticeable trend to change as the river distance changes for the area beside rivers of other grades. Results of this can provide technical support for wetland restoration suitability evaluation for plain areas, a spatial reference for wetland restoration prioritizing, and an orderly wetland development and utilization in future studies and planning.
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http://dx.doi.org/10.3390/ijerph19138083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266254PMC
July 2022

Differential immune response to xenobiotic-modified self-molecule in simple and connective tissue disease-associated primary biliary cholangitis.

Liver Int 2022 Jul 6. Epub 2022 Jul 6.

Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Background And Aims: Our previous studies demonstrated that 2-octynoic acid (2OA) might alter the conformational structure of the inner lipoic acid (LA) binding domain (ILD) in the E2 subunit of pyruvate dehydrogenase complex (PDC-E2), leading to the loss of immune tolerance in simple primary biliary cholangitis (S-PBC). Here, we further explore if this etiological mechanism also accounts for connective tissue disease-associated PBC (CTD-PBC).

Methods: Intein-mediated protein ligation was used to prepare ILD, LA-ILD and 2OA-ILD, and their reactivity with serum samples from 124 S-PBC and 132 CTD-PBC patients was examined. The antibodies to LA, 2OA, LA-ILD and 2OA-ILD, the isotypes of antibodies to LA, 2OA and ILD, were comparatively detected between the two patient groups by enzyme-linked immunosorbent assay and immunoblotting.

Results: Both the percentage and reactivity of antibody to 2OA in S-PBC were significantly higher than in CTD-PBC. Antibodies to 2OA and to LA between the two groups separately shared the same characteristics. Remarkably, coexistence of the antibodies to LA-ILD and to 2OA, and coexistence of the antibodies to LA and to 2OA in S-PBC were both significantly more frequent than in CTD-PBC, whereas the percentage of anti-LA antibody without anti-2OA antibody in S-PBC was markedly lower than in CTD-PBC. Moreover, the isotype of antibody to LA was predominantly IgG in CTD-PBC, whilst this isotype was mainly IgM in S-PBC.

Conclusion: Xenobiotic 2OA might play less important pathogenic role in CTD-PBC than in S-PBC, suggesting that different underlying mechanisms are involved in their immune intolerance to PDC-E2.
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http://dx.doi.org/10.1111/liv.15360DOI Listing
July 2022

Resveratrol, novel application by preconditioning to attenuate myocardial ischemia/reperfusion injury in mice through regulate AMPK pathway and autophagy level.

J Cell Mol Med 2022 Aug 5;26(15):4216-4229. Epub 2022 Jul 5.

Department of Pharmacology, Shantou University Medical College, Shantou, China.

Myocardial ischemia/reperfusion injury (MI/RI) is the main cause of deaths in the worldwide, leading to severe cardiac dysfunction. Resveratrol (RSV) is a polyphenol plant-derived compound. Our study aimed to elucidate the underlying molecular mechanism of preconditioning RSV in protecting against MI/RI. Mice were ligated and re-perfused by the left anterior descending branch with or without RSV (30 mg/kg·ip) for 7 days. Firstly, we found that RSV pretreatment significantly alleviated myocardial infarct size, improved cardiac function and decreased oxidative stress. Furthermore, RSV activated p-AMPK and SIRT1, ameliorated inflammation including the level of TNF-α and IL-1β, and promoting autophagy level. Moreover, neonatal rat ventricular myocytes (NRVMs) and H9c2 cells with knockdown the expression of AMPK, SIRT1 or FOXO1 were used to uncover the underlying molecular mechanism for the cardio-protection of RSV. In NRVMs, RSV increased cellular viability, decreased LDH release and reduced oxidative stress. Importantly, Compound C(CpC) and EX527 reversed the effect of RSV against MI/RI in vivo and in vitro and counteracted the autophagy level induced by RSV. Together, our study indicated that RSV could alleviate oxidative stress in cardiomyocytes through activating AMPK/SIRT1-FOXO1 signallingpathway and enhanced autophagy level, thus presenting high potential protection on MI/RI.
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http://dx.doi.org/10.1111/jcmm.17431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345293PMC
August 2022

Outcomes of Zone 1 Thoracic Endovascular Aortic Repair With Fenestrated Surgeon-Modified Stent-Graft for Aortic Arch Pathologies.

J Endovasc Ther 2022 Jul 2:15266028221108903. Epub 2022 Jul 2.

Division of Vascular Surgery, Department of General Surgery, Changhai Hospital, Naval Medical University, Shanghai, China.

Objectives: This study evaluated the feasibility and safety of zone 1 thoracic endovascular aortic repair (TEVAR) with fenestrated surgeon-modified stent-graft (SMSG) for aortic arch pathologies.

Methods: Between March 2016 and November 2020, 34 consecutive patients underwent zone 1 TEVAR with fenestrated SMSG for aortic arch pathologies. Outcomes included technical success, perioperative, and follow-up morbidity and mortality.

Results: During the study period, 34 patients were treated with zone 1 TEVAR with fenestrated SMSG. Twenty-four (70.6%) patients presented with type B aortic dissections, 9 (26.5%) patients presented with aneurysms (7 located on the lesser curvature side of aortic arch), 1 (2.9%) patient presented with type Ia endoleak after previous TEVAR owing to traumatic aortic dissection. The proximal landing zone for all patients were in zone 1, and all supra-aortic trunks were reconstructed, except for one left subclavian artery. Technical success was achieved in all cases. The 30-day estimated survival (±SE) was 90.9% ± 5.0% [95% confidence interval (CI): 77.0%-97.0%]. The 30-day estimated freedom from reintervention (±SE) was 87.9% ± 5.7% (95% CI: 73.4%-95.3%). At a median follow-up of 48 months (range, 12-68 months), 2 patients died, including 1 aortic-related death and 1 non-aortic-related death. One patient had reintervention 13 months after the operation owing to type Ia endoleak. All supra-aortic trunks were patent. The estimated survival (±SE) during follow-up was 85.1% ± 6.2% (95% CI: 69.9%-93.6%). One (2.7%) patient had stroke. The estimated freedom from reintervention (±SE) during follow-up was 84.2% ± 6.5% (95% CI: 69.9%-93.5%).

Conclusions: Zone 1 TEVAR with fenestrated SMSG is an alternate option for treatment of aortic arch pathologies in experienced centers.
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http://dx.doi.org/10.1177/15266028221108903DOI Listing
July 2022

Insights into the superior resistance of In-CoO-GaO/H-Beta to SO and HO in the selective catalytic reduction of NO by CH.

J Colloid Interface Sci 2022 Jun 25;626:89-100. Epub 2022 Jun 25.

State Key Laboratory of Urban Water Resource and Environment, Shenzhen Key Laboratory of Organic Pollution Prevention and Control, School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, PR China; International Joint Research Center for Persistent Toxic Substances, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, PR China.

The existence of sulfur dioxide and water vapor in the flue gas generated from waste-to-energy stations could lead to catalyst deactivation, which has adverse effects on NO removal. It is thus particularly important to study the reaction mechanism of catalyst resistance to poisoning. Herein, we report the mechanism of In-CoO-GaO/H-Beta catalyst to SO and HO resistance in the selective catalytic reduction (SCR) of NO by CH. The catalyst could achieve 74.6% NO removal efficiency in the presence of 100 ppm SO and 5% HO. In this catalyst, CoO is attributed to enhancing the reversible poisoning of SO and CH activation and increasing the number of Brønsted acid sites by decomposing HO. However, the InO active center was still eroded by a small amount of water vapor, leading to a reduction in NO removal efficiency. The addition of GaO primarily provided an important intermediate NO for CH-SCR reaction and reduced the aggregates of CoO to increase the exposure of indium sites, and could reduce a part of SO to S. This study provides a good candidate for preparing catalysts with superior resistance towards SO and HO for CH-SCR.
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http://dx.doi.org/10.1016/j.jcis.2022.06.106DOI Listing
June 2022

Encoding a WRKY Transcription Factor Enhances Aluminum Tolerance in Soybean.

Int J Mol Sci 2022 Jun 10;23(12). Epub 2022 Jun 10.

The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.

Aluminum (Al) toxicity is an essential factor that adversely limits soybean ( (L.) Merr.) growth in acid soils. WRKY transcription factors play important roles in soybean responses to abiotic stresses. Here, was screened from genes that were differentially expressed under Al treatment in Al-tolerant soybean Baxi10 and Al-sensitive soybean Bendi2. We found that was significantly induced by 20 μM AlCl and upregulated by AlCl treatment for 2 h. In different tissues, the expression of was differentially induced. In 0-1 cm root tips, the expression of was induced to the highest level. The overexpression of in soybean resulted in higher relative root elongation, root weight, depth, root length, volume, number of root tips and peroxidase activity but lower root average diameter, malonaldehyde and HO contents, indicating enhanced Al tolerance. Moreover, RNA-seq identified 205 upregulated and 108 downregulated genes in transgenic lines. Fifteen of these genes that were differentially expressed in both AlCl-treated and -overexpressing soybean had the W-box element, which can bind to the upstream-conserved WRKY domain. Overall, the combined functional analysis indicates that may improve soybean Al tolerance by regulating downstream genes participating in Al transport, organic acid secretion and antioxidant reactions.
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http://dx.doi.org/10.3390/ijms23126518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9224350PMC
June 2022

Integration of full-length transcriptomes and anthocyanin metabolite analysis for understanding fruit coloration mechanism in .

Physiol Mol Biol Plants 2022 May 12;28(5):921-933. Epub 2022 May 12.

College of Horticulture, Shenyang Agricultural University, Shenyang, 110866 China.

Coloration directly affects the commercial value of fruits. The composition and content of anthocyanin determine the fruit coloration. However, the molecular mechanism of anthocyanin biosynthesis and regulation in this fruit remains unknown. In this study, we performed integrative full-length transcriptomics and targeted metabolomics analyses in fruits at four different developmental stages to elucidate the coloration mechanism. Cyanidin3--xyl-rutinoside is the key anthocyanin, which is responsible for the reddening of fruits, and its accumulation gradually accelerated from the 80th day after fluorescence. Overall, 122,289 unigenes with an average length of 2592 bp and an N50 of 4232 bp were obtained through single-molecule real-time sequencing; a total of 16,456 differentially expressed genes were identified. Moreover, 10 full-length structural genes related to anthocyanin biosynthesis were found to be significantly differentially expressed with fruit ripening. Moreover, 10 glycosyltransferases (GTs) that may possess the activities of anthocyanidin 3--glucosyltransferase, anthocyanidin 3--glucoside rhamnosyltransferase, and xylosyltransferases, which are involved in the final three steps for cyanidin3--xyl-rutinoside synthesis, were identified through phylogenetic analysis. Based on these findings, we constructed the complete anthocyanin biosynthetic pathway in fruits; five s, three s, and two s potentially involved in regulating anthocyanin biosynthesis in fruits were also selected. Our study provides the foundation for further research on the molecular mechanism of anthocyanin biosynthesis and regulation for improving the quality of fruits. The results of full-length transcriptomes would provide researchers with novel insights into the molecular cloning of enzymes and their activity.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-022-01179-3.
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http://dx.doi.org/10.1007/s12298-022-01179-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203629PMC
May 2022

Appropriateness and Associated Factors of Stress Ulcer Prophylaxis for Surgical Inpatients of Orthopedics Department in a Tertiary Hospital: A Cross-Sectional Study.

Front Pharmacol 2022 2;13:881063. Epub 2022 Jun 2.

Department of Pharmacy, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Stress ulcer prophylaxis (SUP) prescribed in patients admitted to surgical wards with a low risk of stress-related mucosal disease (SRMD) accounted for a considerable proportion of improper use of proton pump inhibitors (PPIs). This study aimed to analyze the appropriateness of SUP prescribing patterns and identify its associated factors in the orthopedics department of a tertiary hospital in the Northwestern China. In this cross-sectional study, information regarding the demographic and clinical characteristics of 1,200 fracture inpatients who underwent surgical operations from January 2020 to August 2021 were collected from medical records. Established criteria were used to assess the appropriateness of the prescribing pattern for SUP, and the incidence of inappropriate SUP medication was calculated. Logistic regression analyses were used to identify factors associated with inappropriate SUP medication. Approximately, 42.4% of the study population was interpreted as inappropriate prescription of SUP. A total of 397 (33.1%) patients received SUP without a proper indication (overprescription), and the incidence of inappropriate SUP medication was calculated to be 43.11 per 100 patient-days. In addition, 112 (9.3%) inpatients for whom SUP was indicated did not receive SUP (underprescription). PPIs were prescribed in 96.1% of the inpatients who used acid suppression therapy (AST), and intravenous PPIs accounted for 95.3% thereof. In a multivariate logistic regression analysis, age above 65 years and prolonged hospitalization were associated with overprescription of SUP. Increased number of drugs excluding PPIs, the concurrent use of systemic corticosteroids, comorbidity of hypertension, and unemployed or retired status in inpatients were associated with a reduced likelihood of overprescription for SUP. Conversely, prolonged hospitalization, the concurrent use of systemic corticosteroids or anticoagulants, and unemployed status in inpatients were positively associated with underprescription of SUP. There was a high prevalence of inappropriate SUP prescription among noncritically ill inpatients of fracture who underwent surgical operations. We delineated the associated factors with inappropriate SUP medication, which indicated that more information was required for clinicians about rationality and efficiency of their prescribing practices. Effective intervention strategies should be executed by clinical pharmacists to reduce improper SUP medication.
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http://dx.doi.org/10.3389/fphar.2022.881063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203048PMC
June 2022

Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance.

Front Plant Sci 2022 2;13:823529. Epub 2022 Jun 2.

College of Life Sciences, Jilin Agricultural University, Changchun, China.

The gene family is one of the largest gene families in plants. These genes regulate plant growth and development, as well as biotic and abiotic stress responses, and they have been extensively researched. Drought stress is one of the major factors limiting the yield and quality of soybean. In this study, bioinformatics analysis of the soybean gene family was performed, and the role of soybean gene in drought stress adaptation was characterized. We identified 507 genes in the soybean genome database, which were classified into 11 subfamilies. The expression profiles showed that was highly expressed in plant roots. Overexpression of increased the sensitivity of transgenic to drought stress. Under drought stress, the hydrogen peroxide (HO) and malonaldehyde (MDA) contents of transgenic were higher than those of the wild type (WT) and empty vector control, and the chlorophyll content was lower than that of the control. Y2H and bimolecular fluorescence complementation (BiFC) assays showed that GmFBL144 can interact with GmsHSP. Furthermore, our results showed that GmFBL144 can form SCF (E3 ubiquitin ligase) with GmSkp1 and GmCullin1. Altogether, these results indicate that the soybean F-box-like protein GmFBL144 may negatively regulate plant drought stress tolerance by interacting with sHSP. These findings provide a basis for molecular genetics and breeding of soybean.
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http://dx.doi.org/10.3389/fpls.2022.823529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201338PMC
June 2022

Geniposide suppresses NLRP3 inflammasome-mediated pyroptosis via the AMPK signaling pathway to mitigate myocardial ischemia/reperfusion injury.

Chin Med 2022 Jun 17;17(1):73. Epub 2022 Jun 17.

Department of Pharmacology, Shantou University Medical College, Shantou, 515041, China.

Background: NLRP3 inflammasome activation and pyroptosis play a significant role in myocardial ischemia reperfusion injury (MI/RI). Geniposide was reported to show potential therapeutic use for MI/RI with its anti-inflammatory and anti-oxidative properties. However, research on the specific mechanism of geniposide has not been reported.

Methods: The MIRI model of animal was created in male C57BL/6J mice and the hypoxia reoxygenation (H/R) model was established for the in vitro experiments. Neonatal rat ventricular myocytes (NRVMs) and H9c2 cells with knockdown of TXNIP or NLRP3 were used. Geniposide was administered to mice before vascular ligation. HE staining, 2,3,5-triphenyltetrazolium chloride (TTC) staining, echocardiography, oxidative stress and myocardial enzyme detection were used to evaluate the cardioprotective effect of geniposide. Meanwhile, pharmacological approaches of agonist and inhibitor were used to observe potential pathway for geniposide cardioprotective in vitro and in vivo. Moreover, ELISA kits were adopted to detect the levels of inflammatory factors, such as IL-1β and IL-18. The gene and protein expression of NLRP3 and pyroptosis-related factors in heart tissue were performed by RT-PCR, western blotting and immunofluorescence in vivo and in vitro, respectively.

Results: Our results indicate that geniposide can reduce the area of myocardial infarction, improve heart function, and inhibit the inflammatory response in mice after MI/RI. In addition, RT-PCR and western blotting shown geniposide promoting AMPK phosphorylation to activate myocardium energy metabolism and reducing the levels of genes and proteins expression of NLRP3, ASC, N-GSDMD and cleaved caspase-1, IL-1β, IL-18. Meanwhile, geniposide improved NRVMs energy metabolism, which decreased ROS levels and the protein expression of TXNIP and thus suppressed the expression of NLRP3. AMPK antagonist or agonist and siRNA downregulation of TXNIP or NLRP3 were also verify the effect of geniposide against H/R injury. Further research found that geniposide promoted the translocation of TXNIP and reduce the binding of TXNIP and NLRP3.

Conclusions: In our study, geniposide can significantly inhibit NLRP3 inflammasome activation via the AMPK signaling pathway and inhibit pyroptosis of cardiomyocytes in myocardial tissues.
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http://dx.doi.org/10.1186/s13020-022-00616-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205109PMC
June 2022

Learning from errors? The impact of erroneous example elaboration on learning outcomes of medical statistics in Chinese medical students.

BMC Med Educ 2022 Jun 17;22(1):469. Epub 2022 Jun 17.

Department of Oral and Cranio-maxillofacial Surgery, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.

Background: Constructivism theory has suggested that constructing students' own meaning is essential to successful learning. The erroneous example can easily trigger learners' confusion and metacognition, which may "force" students to process the learning material and construct meaning deeply. However, some learners exhibit a low level of elaboration activity and spend little time on each example. Providing instructional scaffolding and elaboration training may be an efficient method for addressing this issue. The current study conducted a randomized controlled trial to examine the effectiveness of erroneous example elaboration training on learning outcomes and the mediating effects of metacognitive load for Chinese students in medical statistics during the COVID-19 pandemic.

Methods: Ninety-one third-year undergraduate medical students were randomly assigned to the training group (n = 47) and the control group (n = 44). Prerequisite course performance and learning motivation were collected as covariates. The mid-term exam and final exam were viewed as posttest and delayed-test to make sure the robustness of the training effect. The metacognitive load was measured as a mediating variable to explain the relationship between the training and academic performance.

Results: The training significantly improved both posttest and delayed-test performance compared with no training (F = 26.65, p < 0.001, Partial η = 0.23; F = 38.03, p < 0.001, Partial η = 0.30). The variation trend in metacognitive load in the two groups was significantly different (F = 2.24, p < 0.05, partial η = 0.20), but metacognitive load could not explain the positive association between the treatment and academic performance (β = - 0.06, se = 0.24, 95% CI - 0.57 to 0.43).

Conclusions: Erroneous example learning and metacognitive demonstrations are effective for academic performance in the domain of medical statistics, but their underlying mechanism merits further study.
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http://dx.doi.org/10.1186/s12909-022-03460-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203230PMC
June 2022

Activating cGAS-STING axis contributes to neuroinflammation in CVST mouse model and induces inflammasome activation and microglia pyroptosis.

J Neuroinflammation 2022 Jun 10;19(1):137. Epub 2022 Jun 10.

Department of Cerebrovascular Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, No 600 Tianhe Road, Guangzhou, 510630, Guangdong, China.

Background: Neuroinflammation-induced injury is intimately associated with poor prognosis in patients with cerebral venous sinus thrombosis (CVST). The cyclic GMP-AMP synthase-stimulator of interferon gene (cGAS-STING) axis is a cytoplasmic double-stranded DNA (dsDNA) sensing pathway has recently emerged as a crucial mediator of neuroinflammation in ischemic stroke. However, the role of the cGAS-STING pathway in modulating post-CVST inflammation and the underlying mechanisms involved remain unclear.

Methods: A CVST model was induced by ferric chloride in male C57BL/6J mice. The selective cGAS inhibitor RU.521, STING agonist 2'3'-cGAMP, and STING siRNA were delivered by intranasal administration or intraventricular injection. Post-CVST assessments included rotarod test, TUNEL staining, Fluoro-Jade C staining, dihydroethidium staining, western blotting, qPCR, immunofluorescence, immunohistochemistry, ELISA and flow cytometry.

Results: cGAS, STING, NLRP3 and GSDMD were significantly upregulated after CVST and mostly in the microglia of the mouse brain. CVST triggered the release of dsDNA into the cytoplasm and elicited an inflammatory response via activating the cGAS-STING axis. RU.521 decreased the levels of 2'3'-cGAMP, STING and downstream inflammatory cytokines, and suppressed the expressions of NLRP3 inflammasome and pyroptosis-pertinent components containing cleaved caspase-1, GSDMD, GSDMD-C, pro- and cleaved IL-1β, and cleaved IL-1β/pro-IL-1β. Besides, RU.521 treatment also reduced oxidative stress, lessened the numbers of microglia and neutrophils, and ameliorated neuronal apoptosis, degeneration along with neurological deficits post-CVST. 2'3'-cGAMP delivery enhanced the expressions of STING and related inflammatory mediators, NLRP3 inflammasome and pyroptosis-relevant proteins, whereas these alterations were significantly abrogated by the silencing of STING by siRNA.

Conclusions: Our data demonstrate that repression of the cGAS-STING pathway diminishes the neuroinflammatory burden of CVST and highlight this approach as a potential therapeutic tactic in CVST-mediated pathologies.
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http://dx.doi.org/10.1186/s12974-022-02511-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9188164PMC
June 2022

A fast responsive and cell membrane-targetable near-infrared HS fluorescent probe for drug resistance bioassays in chemotherapy.

Chem Commun (Camb) 2022 Jun 28;58(52):7301-7304. Epub 2022 Jun 28.

Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science & Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, South-Central Minzu University, Wuhan 430074, China.

A cell membrane-targeted near-infrared fluorescent probe, CMCu-HS, was fabricated through employing hydrophobic chains and cyclen-Cu as targeting and recognition groups, respectively. NIR fluorescence of CMCu-HS can significantly increase after reacting with HS by removing the quenchable Cu. This probe exhibited high selectivity and an extremely fast response rate. Cell imaging results demonstrated that there was a close relationship between the overexpression of NFS1 and drug resistance and inhibition of NFS1 was beneficial for improving the efficacy of chemotherapy.
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http://dx.doi.org/10.1039/d2cc02430fDOI Listing
June 2022

Comparing effects of microgravity and amyotrophic lateral sclerosis in the mouse ventral lumbar spinal cord.

Mol Cell Neurosci 2022 Jul 2;121:103745. Epub 2022 Jun 2.

Laboratory of Neurobiology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan; Department of Neurobiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.

Microgravity (MG) exposure and motor neuron diseases, such as amyotrophic lateral sclerosis (ALS), lead to motor deficits, including muscle atrophy and loss of neuronal activity. Abnormalities in motor neurons and muscles caused by MG exposure can be recovered by subsequent ground exercise. In contrast, the degeneration that occurs in ALS is irreversible. A common phenotype between MG exposure and ALS pathology is motor system abnormality, but the causes may be different. In this study, to elucidate the motor system that is affected by each condition, we investigated the effects of MG and the human SOD1 ALS mutation on gene expression in various cell types of the mouse ventral lumbar spinal cord, which is rich in motor neurons innervating the lower limb. To identify cell types affected by MG or ALS pathogenesis, we analyzed differentially expressed genes with known cell-type markers, which were determined from previous single-cell studies of the spinal cord in MG-exposed and SOD1 mice, an ALS mouse model. Differentially expressed genes were observed in MG mice in various spinal cord cell types, including neurons, microglia, astrocytes, oligodendrocytes, oligodendrocyte precursor cells, meningeal cells/Schwann cells, and vascular cells. We also examined neuronal populations in the spinal cord. Gene expression in putative excitatory and inhibitory neurons changed more than that in cholinergic motor neurons of the spinal cord in both MG and SOD1 mice. Many putative neuron types, especially visceral motor neurons, and axon initial segments (AIS) were affected in MG mice. In contrast, the effect on neurons and AIS in SOD1 mice was slight at P30 but progressed with aging. Interestingly, changes in dopaminergic system-related genes were specifically altered in the spinal cord of MG mice. These results indicate that MG and ALS pathology in various cell types contribute to motor neuron degeneration. Furthermore, there were more alterations in neurons in MG-exposed mice than in SOD1 mice. A large number of differentially expressed genes (DEGs) in MG mice represent more than SOD1 mice with ALS pathology. Elucidation of MG pathogenesis may provide more insight into the pathophysiology of neurodegenerative diseases.
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http://dx.doi.org/10.1016/j.mcn.2022.103745DOI Listing
July 2022

Which Frequency of Ankle Pump Exercise Should Be Chosen for the Prophylaxis of Deep Vein Thrombosis?

Inquiry 2022 Jan-Dec;59:469580221105989

Department of Clinical Nursing, Nursing School, Naval Medical University, Shanghai, China.

Introduction: Ankle pump exercise (APE) is an effective method to prevent deep vein thrombosis (DVT) in the lower extremities. However, traditional APE at a frequency of 3 times/min applied in the clinic lacked high compliance, and individuals were found to prefer an APE of 30 times/min. This study aimed to compare the effects of APEs with the above two different frequencies on the venous hemodynamics and fatigue of lower extremities.

Methods: The study applied a randomized cross-over design, and 307 healthy adults were randomly assigned into two groups, whose left feet performed 5 minutes of traditional APE (3 times/min, after 10 minutes of rest) and 5 minutes of selected APE (30 times/min, after 10 minutes of rest) in different orders. The diameters and blood flow velocities of the external iliac vein, femoral vein, and popliteal vein of the left extremities were recorded with the color doppler ultrasound when participants were at rest and after 5 minutes of traditional or selected APE. The fatigue of lower extremities was assessed with the Rating of Perceived Exertion (RPE) after the participants performed 5 minutes of different APEs.

Results: After both traditional APE and selected APE, the diameters and blood flow (peak systolic) velocities of the external iliac vein, femoral vein, and popliteal vein increased significantly in the participants (P<.01), and the effects of the two APEs on venous hemodynamics of the lower extremities had no significant difference (P>.05). However, participants reported that traditional APE caused higher fatigue, and 252 (82.1%) participants preferred APE at 30 times/min.

Conclusion: Both traditional and selected APE could increase venous blood flow in the lower extremity. Despite the equivalent effects, the selected APE of 30 times/min may cause less fatigue than the traditional one of 3 times/min.
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http://dx.doi.org/10.1177/00469580221105989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168852PMC
June 2022

Metabonomic Characteristics of Myocardial Diastolic Dysfunction in Type 2 Diabetic Cardiomyopathy Patients.

Front Physiol 2022 9;13:863347. Epub 2022 May 9.

Department of Endocrinology, Shenzhen Clinical Research Center for Metabolic Diseases, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center of Shenzhen University, Shenzhen, China.

Diabetic cardiomyopathy (DCM) is one of the most essential cardiovascular complications in diabetic patients associated with glucose and lipid metabolism disorder, fibrosis, oxidative stress, and inflammation in cardiomyocytes. Despite increasing research on the molecular pathogenesis of DCM, it is still unclear whether metabolic pathways and alterations are probably involved in the development of DCM. This study aims to characterize the metabolites of DCM and to identify the relationship between metabolites and their biological processes or biological states through untargeted metabolic profiling. UPLC-MS/MS was applied to profile plasma metabolites from 78 patients with diabetes (39 diabetes with DCM and 39 diabetes without DCM as controls). A total of 2,806 biochemical were detected. Compared to those of DM patients, 78 differential metabolites in the positive-ion mode were identified in DCM patients, including 33 up-regulated and 45 down-regulated metabolites; however, there were only six differential metabolites identified in the negative mode including four up-regulated and two down-regulated metabolites. Alterations of several serum metabolites, including lipids and lipid-like molecules, organic acids and derivatives, organic oxygen compounds, benzenoids, phenylpropanoids and polyketides, and organoheterocyclic compounds, were associated with the development of DCM. KEGG enrichment analysis showed that there were three signaling pathways (metabolic pathways, porphyrin, chlorophyll metabolism, and lysine degradation) that were changed in both negative- and positive-ion modes. Our results demonstrated that differential metabolites and lipids have specific effects on DCM. These results expanded our understanding of the metabolic characteristics of DCM and may provide a clue in the future investigation of reducing the incidence of DCM. Furthermore, the metabolites identified here may provide clues for clinical management and the development of effective drugs.
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http://dx.doi.org/10.3389/fphys.2022.863347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150260PMC
May 2022

Modeling and Preliminary Analysis of the Impact of Meteorological Conditions on the COVID-19 Epidemic.

Int J Environ Res Public Health 2022 05 18;19(10). Epub 2022 May 18.

School of Atmospheric Sciences and Key Laboratory of Tropical Atmosphere-Ocean System, Ministry of Education, Sun Yat-Sen University, Zhuhai 519082, China.

Since the COVID-19 epidemic outbreak at the end of 2019, many studies regarding the impact of meteorological factors on the attack have been carried out, and inconsistent conclusions have been reached, indicating the issue's complexity. To more accurately identify the effects and patterns of meteorological factors on the epidemic, we used a combination of logistic regression (LgR) and partial least squares regression (PLSR) modeling to investigate the possible effects of common meteorological factors, including air temperature, relative humidity, wind speed, and surface pressure, on the transmission of the COVID-19 epidemic. Our analysis shows that: (1) Different countries and regions show spatial heterogeneity in the number of diagnosed patients of the epidemic, but this can be roughly classified into three types: "continuous growth", "staged shock", and "finished"; (2) Air temperature is the most significant meteorological factor influencing the transmission of the COVID-19 epidemic. Except for a few areas, regional air temperature changes and the transmission of the epidemic show a significant positive correlation, i.e., an increase in air temperature is conducive to the spread of the epidemic; (3) In different countries and regions studied, wind speed, relative humidity, and surface pressure show inconsistent correlation (and significance) with the number of diagnosed cases but show some regularity.
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http://dx.doi.org/10.3390/ijerph19106125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140896PMC
May 2022

Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in .

Genes (Basel) 2022 May 4;13(5). Epub 2022 May 4.

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.

is an important medicinal plant, which is mainly used for treatment of cardiovascular and cerebrovascular diseases. GATA transcription factors are evolutionarily conser-ved proteins that play essential roles in biological process of plants. In this study, we systematically characterized the GATA transcription factors in . A total 28 genes were identified and divided into four subfamilies based on phylogenetic analysis and domain. genes being clustered into a subfamily have similar conserved motifs and exon-intron patterns, and unevenly distribute on eight chromosomes of . Tissue-specific expression analysis based on transcriptome datasets showed that the majority of genes were preferentially expressed in roots. Under methyl jasmonate (MeJA) treatment, the quantitative real-time PCR (qRT-PCR) analysis indicated that several genes in roots showed distinct upregulation post-MeJA treatment, especially , which was highly responsive to MeJA, and might be involved in the jasmonate signal, thereby affecting root growth, development, tolerance to various stresses, or secondary metabolites biosynthesis. The study found that several s, like , are highly responsive to MeJA, indicating that these s might be vital in the biosynthesis of tanshinones and phenolic acids by regulating the response to MeJA in . Our results laid the foundation for understanding their biological roles and quality improvement in .
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http://dx.doi.org/10.3390/genes13050822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140432PMC
May 2022

Study on the Structure, Magnetic Properties and Mechanism of Zn-Doped Yttrium Iron Garnet Nanomaterial Prepared by the Sol-gel Method.

Gels 2022 May 23;8(5). Epub 2022 May 23.

China's People's Liberation Army 32319 Troops, Urumqi 830001, China.

To explore the effect and mechanism of bivalent ion doping on yttrium iron garnet (YIG), Zn-YIG (Zn-doped YIG) nanoparticles with a size of 60~70 nm were prepared by the sol-gel method. It was proven that Zn ion doping resulted in lattice expansion and internal stress due to crystallite size shrinkage. A Raman spectroscopic analysis proved the influence of Zn doping on the crystal structure and peak intensity by analyzing Raman vibration modes. The characteristics and chemical mechanism of mass loss and phase evolution in each temperature region were explored through TG-DSC measurements. Moreover, it was revealed that the antiferromagnetic coupling, pinning mechanisms and particle aggregation lead to coercivity, exhibiting different variation trends. A saturation magnetization () curve variation mechanism was further revealed, which was due to the thermal effects, super-exchange effect, and coupling effect between sub-lattices. Meanwhile, the influence of the thermal effect on and its mechanism were explored by spin theory, and it was proven that it was mainly caused by the random arrangement of magnetic moments and thermal vibration. These results provide theoretical support for the wider application of YIG devices in microwave and high-temperature fields.
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http://dx.doi.org/10.3390/gels8050325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140988PMC
May 2022
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