Publications by authors named "Haiyan Cui"

98 Publications

Corrigendum to: An Innovative Approach for Facial Rejuvenation and Contouring Injections in Asian Patients.

Aesthet Surg J Open Forum 2021 Sep 10;3(3):ojab024. Epub 2021 Jul 10.

[This corrects the article DOI: 10.1093/asjof/ojaa053.][This corrects the article DOI: 10.1093/asjof/ojab011.].
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http://dx.doi.org/10.1093/asjof/ojab024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272525PMC
September 2021

An Innovative Approach for Facial Rejuvenation and Contouring Injections in Asian Patients.

Aesthet Surg J Open Forum 2021 Jun 13;3(2):ojaa053. Epub 2021 Feb 13.

Hangzhou Time Plastic and Cosmetology Hospital, Hangzhou, China.

An increasing number of Asian people are seeking nonsurgical facial aesthetic treatments. Ethnic Asians differ from Western populations in both facial appearance and baseline structural facial anatomy. And there is a lack of clinical instruction to doctors who provide facial aesthetic treatment for Asian patients. The authors proposed the " Future Codes" design in Chinese calligraphy describing the art of facial injection in Asians to help doctors perform well. "" are pictograph of 2 Chinese characters, translated into English as "Future," which represent beautiful meanings and vividly describe the procedure and operating area of the design methods. The concept encompasses a systematic overall design for the art of facial injection in Asians, and these procedures are easy to learn and perform safely. This is the first systematic solution available in the clinic that can be used to design facial aesthetics and rejuvenation in Asians through Eastern philosophy and culture.

Level Of Evidence 5:
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http://dx.doi.org/10.1093/asjof/ojaa053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240745PMC
June 2021

Reproducibility of vaginal immobilization balloons overnight for cervical cancer brachytherapy.

J Contemp Brachytherapy 2021 Jun 13;13(3):280-285. Epub 2021 May 13.

Department of Radiation Oncology, University of Arizona College of Medicine, Tucson, USA.

Purpose: The use of vaginal immobilization balloons placed into the vagina for immobilization of tandem and ovoid (T+O) applicator during high-dose-rate (HDR) brachytherapy delivery has been used at our institution, and seems to have improved our patient comfort, decreased procedure time, and minimized applicator misplacement. We aimed to show that these balloons, while originally marketed for single-day use, are safe and maintain applicator positioning/dosimetry when left overnight for treatment delivery on sequential days.

Material And Methods: Forty-two paired computed tomography (CT) scans from thirteen patients who underwent T+O HDR treatments on sequential days with vaginal immobilization balloons overnight were retrospectively compared to calculate mean change of balloon volumes and balloon/T+O distance to bony landmarks. Dosimetric planning was retroactively performed on day 2 using CT scan of each pair, and the change in estimated radiation delivery to the bladder and rectum was compared.

Results: No statistically significant overnight changes were found in balloon volumes or anterior balloon positioning. The posterior balloon shifted -0.29 ±0.46 cm ( = 0.03) to the anterior public symphysis and 0.32 ±0.50 cm ( = 0.01) to the right femoral head. The tandem shifted 0.37 ±0.39 cm ( = 0.002) to the pubic symphysis. There was no significant difference found in radiation delivered to the bladder or rectum between the paired scans.

Conclusions: This study showed minimal change in balloon volumes, balloons/T+O positioning, or in radiation dose to bladder and rectum when the applicator remained overnight. These findings support that inflatable vaginal immobilization balloons remaining overnight for additional HDR T+O treatments on sequential days, is safe and provides stable dosimetry.
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http://dx.doi.org/10.5114/jcb.2021.106117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170519PMC
June 2021

Copper Peroxide-Loaded Gelatin Sponges for Wound Dressings with Antimicrobial and Accelerating Healing Properties.

ACS Appl Mater Interfaces 2021 Jun 7;13(23):26800-26807. Epub 2021 Jun 7.

State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, 222 Tianshui Road, Lanzhou 730000, P. R. China.

Catalytic conversion of hydrogen peroxide (HO) to more toxic hydroxyl radicals (OH) is a good choice for sterilization and anti-infection, but endogenous HO is insufficient to achieve satisfactory sterilization efficacy. Despite great efforts, designing and developing antimicrobial materials that specifically and effectively self-supply HO at the wound site remain as tremendous challenges. Here, we report a pH-responsive copper peroxide-loaded wound dressing made from copper hydroxide and gelatin sponge and then reacted with HO. In vitro experiments show that the prepared wound dressing has good bactericidal properties against (), (), and (). Moreover, the as-prepared wound dressing can release OH specifically in the bacterial-infected skin wound, rather than in normal tissues, and in vivo skin wound-healing experiments proved that the synthesized copper peroxide-loaded gelatin sponge could combat effectively; in addition, Cu released from the gelatin sponge could stimulate angiogenesis and collagen deposition simultaneously. The study provides a strategy to improve antibacterial efficacy and reduce the toxic side effects through the release of OH by bacterial self-activation.
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http://dx.doi.org/10.1021/acsami.1c07409DOI Listing
June 2021

Highly Sensitive and Selective Photoelectrochemical Aptasensors for Cancer Biomarkers Based on MoS/Au/GaN Photoelectrodes.

Anal Chem 2021 05 7;93(19):7341-7347. Epub 2021 May 7.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350116, China.

An Au/GaN photoelectrode was prepared by sputtering 30 nm thick Au film on the surface of n-type gallium nitride (GaN). When the electrode contacts with multilayered molybdenum disulfide (MoS), photogenerated electrons and photogenerated holes transfer to MoS because of the band gap matching of MoS and GaN. The presence of Au promotes charge transfer and results in a greater recombination of electrons and holes; by this means, a more significant suppression of photocurrent can be detected. This characteristic has been coupled with the high selectivity of an aptamer and applied to develop a novel photoelectrochemical aptasensor for cancer biomarkers (alpha-fetoprotein (AFP) as a model). The aptamer of AFP was modified on the surface of the Au/GaN photoelectrode by Au-S bonds, which can bind to the target protein with high selectivity. Then, the transfer process of the charge carriers of GaN to MoS can be blocked by the target protein so that the suppression of photocurrent is reduced. The difference of the photocurrent in the presence and absence of AFP () showed a linear relationship with AFP concentration that ranged from 1.0-150 ng/mL ( = 0.9995), and the detection limit was 0.3 ng/mL. The standard addition recovery rates ranged from 85.2 to 91.7%. The method possessed good sensitivity and high selectivity for AFP detection. The developed biosensor can be modified to detect other cancer biomarkers by simply replacing the aptamer used.
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http://dx.doi.org/10.1021/acs.analchem.1c01197DOI Listing
May 2021

Performance of Interferon-Gamma Release Assays in the Diagnosis of Nontuberculous Mycobacterial Diseases-A Retrospective Survey From 2011 to 2019.

Front Cell Infect Microbiol 2020 18;10:571230. Epub 2021 Feb 18.

Shanghai Clinical Research Center for Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

There is an urgent need for precise diagnosis to distinguish nontuberculous mycobacterial (NTM) diseases from pulmonary tuberculosis (PTB) and other respiratory diseases. The aim of this study is to evaluate the diagnostic performance of Interferon-gamma (IFN-γ) release assays (IGRAs), including antigen-specific peripheral blood-based quantitative T cell assay (T-SPOT.TB) and QuantiFERON-TB-Gold-Test (QFT-G), in differentiating NTM infections ( = 1,407) from culture-confirmed PTB ( = 1,828) and other respiratory diseases ( = 2,652). At specie level, 2.56%, 10.73%, and 16.49% of NTM-infected patients were infected by , , and with - complex (MAC), respectively. Valid analyses of T-SPOT.TB (ESAT-6, CFP-10) and QFT-G were available for 37.03% and 85.79% in NTM-infected patients, including zero and 100% (36/36) of infection, 21.85% (33/151) and 92.05% (139/151) of infection, and 17.67% (41/232) and 91.24% (211/232) of MAC infection. Based on means comparisons and further ROC analysis, T-SPOT.TB and QFT-G performed moderate accuracy when discriminating NTM from PTB at modified cut-off values (ESAT-6 < 4 SFCs, CFP-10 < 3 SFCs, and QFT-G < 0.667 IU/ml), with corresponding AUC values of 0.7560, 0.7699, and 0.856. At species level of NTM, QFT-G effectively distinguished between MAC (AUC=0.8778), (AUC=0.8834) or (AUC=0.8783) than T-SPOT.TB. No significant differences in discriminatory power of these three IGRA tools were observed when differentiating NTM and Controls. Our results demonstrated that T-SPOT.TB and QFT-G were both efficient methods for differentiating NTM disease from PTB, and QFT-G possessed sufficient discriminatory power to distinguish infections by different NTM species.
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http://dx.doi.org/10.3389/fcimb.2020.571230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930076PMC
June 2021

Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2.

Aging (Albany NY) 2021 01 13;13(2):2941-2958. Epub 2021 Jan 13.

Department of Pathology, Zibo Central Hospital, Zibo 255000, Shandong Province, China.

Gallbladder carcinoma (GC) is an extremely malignant gastrointestinal tumor, but relevant mechanisms are still under investigation. MicroRNA (miR) is differentially expressed in a variety of tumors. Here we explored miR-204 in patients with GC and related mechanisms. A GSE104165 chip was downloaded from the gene expression omnibus (GEO) for analysis. The qRT-PCR assay was used for quantifying miR-204 and Notch2 in the serum and tissues of the patients, and the patients were followed up for 3 years to analyze independent factors of prognosis. The CCK8, transwell, and flow cytometry assays were applied for analyzing proliferation, invasion, as well as apoptosis of cells, and the dual luciferase reporter (DLR) assay was adopted for determining the association of miR-204 with Notch2. MiR-204 was low in patients with GC, and it might serve as a diagnostic indicator for GC. In addition, patients with low e MiR-204 usually faced high rates of III+IV stage, distant metastasis, and low differentiation, and also showed a poor prognosis. DLR assay verified the targeted binding of miR-204 to Notch2 mRNA.
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http://dx.doi.org/10.18632/aging.202444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880336PMC
January 2021

Air pollution and critical air pollutant assessment during and after COVID-19 lockdowns: Evidence from pandemic hotspots in China, the Republic of Korea, Japan, and India.

Atmos Pollut Res 2021 Feb 28;12(2):316-329. Epub 2020 Nov 28.

School of Public Health, Shanxi Medical University, No. 56 Xinjian South Street, Taiyuan, 030001, China.

The COVID-19 virus outbreak has been declared a "global pandemic". Therefore, "lockdown" was issued in affected countries to control the spread of the virus. To assess air pollution during and after lockdowns, this study selected pandemic hotspots in China (Wuhan), Japan (Tokyo), the Republic of Korea (Daegu), and India (Mumbai) and compared the Air Quality Index (AQI) in these areas for the past three years. The results indicated that air pollution levels were positively correlated with a reduction in pollutant levels during and after lockdowns in these cities. In Tokyo, low levels of air pollution, no significant change in the distribution of "good" and "moderate" days was observed during lockdown. In Daegu, mid-level air pollution, the percentage of "unhealthy" days (AQI>100) markedly reduced during lockdown; however, this reverted after lockdown was lifted. In Wuhan and Mumbai, high air pollution levels, the percentage of unhealthy days remarkably decreased during lockdown and continued to reduce after lockdown. It was found that PM was the critical pollutant for all cities because its sub-AQI was the largest of the six pollutant species for the majority of days. In addition, PM dominated the overall AQI for 2.2-9.6% of the period in Wuhan and Mumbai, and its sub-AQI reduced during lockdown. The mean sub-AQI for NO, SO, CO, and O was within the "good" category for all cities. In conclusion, the lockdown policy reduced air pollution in general and this reduction was more significant for regions with high air pollution levels.
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http://dx.doi.org/10.1016/j.apr.2020.11.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695571PMC
February 2021

Formaldehyde-induced hematopoietic stem and progenitor cell toxicity in mouse lung and nose.

Arch Toxicol 2021 02 21;95(2):693-701. Epub 2020 Oct 21.

Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, CA, USA.

Formaldehyde (FA), an economically important and ubiquitous chemical, has been classified as a human carcinogen and myeloid leukemogen. However, the underlying mechanisms of leukemogenesis remain unclear. Unlike many classical leukemogens that damage hematopoietic stem/progenitor cells (HSC/HPC) directly in the bone marrow, FA-as the smallest, most reactive aldehyde-is thought to be incapable of reaching the bone marrow through inhalation exposure. A recent breakthrough study discovered that mouse lung contains functional HSC/HPC that can produce blood cells and travel bi-directionally between the lung and bone marrow, while another early study reported the presence of HSC/HPC in rat nose. Based on these findings, we hypothesized that FA inhalation could induce toxicity in HSC/HPC present in mouse lung and/or nose rather than in the bone marrow. To test this hypothesis, we adapted a commercially available protocol for culturing burst-forming unit-erythroid (BFU-E) and colony-forming unit-granulocyte, macrophage (CFU-GM) colonies from bone marrow and spleen to also enable culture of these colonies from mouse lung and nose, a novel application of this assay. We reported that in vivo exposure to FA at 3 mg/m or ex vivo exposure up to 400 µM FA decreased the formation of both colony types from mouse lung and nose as well as from bone marrow and spleen. These findings, to the best of our knowledge, are the first empirically to show that FA exposure can damage mouse pulmonary and olfactory HSC/HPC and provide potential biological plausibility for the induction of leukemia at the sites of entry rather than the bone marrow.
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http://dx.doi.org/10.1007/s00204-020-02932-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878325PMC
February 2021

The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.

Mol Med Rep 2020 Nov 21;22(5):3723-3734. Epub 2020 Aug 21.

Department of Respiratory and Critical Care Medicine, Chronic Airway Disease Laboratory, Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510000, P.R. China.

The cellular and molecular mechanisms via which MK2206, an AKT inhibitor, prevents the activation of AKT in toluene diisocyanate (TDI)‑induced asthma remain unclear. Thus, the present study aimed to evaluate the potential effects of MK2206 on airway AKT activation, inflammation and remodeling in a TDI‑induced mouse model of asthma. A total of 24 BALB/c mice were selected and randomly divided into untreated (AOO), asthma (TDI), MK2206 (TDI + MK2206), and dexamethasone (TDI + DEX) groups. Phosphorylated AKT (p‑AKT), total AKT, airway remodeling indices, α‑smooth muscle actin (α‑SMA) and collagen I levels in pulmonary tissue were measured using western blotting. Airway inflammation factors, including interleukin (IL)‑4, ‑5, ‑6, and ‑13 in bronchoalveolar lavage fluid (BALF) and IgE in serum, were determined using ELISA. Additionally, the airway hyperresponsiveness (AHR) and pulmonary pathology of all groups were evaluated. The results of the present study demonstrated that p‑AKT levels in lung protein lysate were upregulated, and neutrophil, eosinophil and lymphocyte counts were increased in the lungs obtained from the asthma group compared with the AOO group. Both MK2206 and DEX treatment in TDI‑induced mice resulted not only in the attenuation of AKT phosphorylation, but also reductions in neutrophil, eosinophil and lymphocyte counts in the lungs of mice in the asthma group. Consistently, increases in the levels of the inflammatory cytokines IL‑4, ‑5, ‑6 and ‑13 analyzed in BALF, and serum IgE in the TDI group were demonstrated to be attenuated in the TDI + MK2206 and TDI + DEX groups. Furthermore, α‑SMA and AHR were significantly attenuated in the TDI + MK2206 group compared with the TDI group. These results revealed that MK2206 not only inhibited AKT activation, but also served a role in downregulating airway inflammation and airway remodeling in chemical‑induced asthma. Therefore, the findings of the present study may provide important insight into further combination therapy.
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http://dx.doi.org/10.3892/mmr.2020.11450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533517PMC
November 2020

Triphenyl phosphate exposure induces kidney structural damage and gut microbiota disorders in mice under different diets.

Environ Int 2020 11 17;144:106054. Epub 2020 Aug 17.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, 163 Xianlin Avenue, Nanjing 210023, China. Electronic address:

Exposure of humans to organophosphate flame retardants (OPFRs) and the consequent health risk have increased owing to the latter's widespread application. Although triphenyl phosphate (TPP), an OPFR, is a potential chemical determinant of liver function damage, its effects on kidney function in mice under high fructose/fat (HFF) diet are still unclear. In this study, C57BL/6J mice were fed HFF to generate an obesity model and mice were exposed to low dose (0.01 mg/kg/day; TPP-L) and high dose (1 mg/kg/day; TPP-H) of TPP for 12 weeks. Results showed that TPP-L and TPP-H combined with HFF, as well as TPP-H alone, caused kidney structural damage and gut microbiota disorders in mice. Inflammatory response induced by nuclear factor kappa B (NF-κB p65)/nod-like receptor protein 3 (NLRP3) and caspase-3 promoted kidney structure damage, as well as accumulation of triglyceride and total cholesterol and the protein residues in urine. Although TPP-L did not cause obvious structural damage in the kidneys, 0.01 mg/kg TPP induced significant inflammation and gut microbiota disorders. These findings provide new insights regarding health risk assessment after chronic exposure to TPP and HFF alone, as well as a combination of TPP with HFF in mice.
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http://dx.doi.org/10.1016/j.envint.2020.106054DOI Listing
November 2020

Vaginal microbiota, genital inflammation, and neoplasia impact immune checkpoint protein profiles in the cervicovaginal microenvironment.

NPJ Precis Oncol 2020 3;4:22. Epub 2020 Aug 3.

Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona, Phoenix, AZ USA.

Emerging evidence suggests that the vaginal microbiota play a role in HPV persistence and cervical neoplasia development and progression. Here we examine a broad range of immune checkpoint proteins in the cervicovaginal microenvironment across cervical carcinogenesis and explore relationships among these key immunoregulatory proteins, the microbiota composition, and genital inflammation. First, we demonstrate that immune checkpoint molecules can be measured in cervicovaginal lavages. Secondly, we identify CD40, CD27, and TIM-3 to specifically discriminate cervical cancer from other groups and CD40, CD28, and TLR2 to positively correlate to genital inflammation. Finally, PD-L1 and LAG-3 levels negatively, whereas TLR2 positively correlate to health-associated dominance. Overall, our study identifies immune checkpoint signatures associated with cervical neoplasm and illuminates the multifaceted microbiota-host immunity network in the local microenvironment. This study provides a foundation for future mechanistic studies and highlights the utility of cervicovaginal lavage profiling for predicting and monitoring response to cancer therapy.
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http://dx.doi.org/10.1038/s41698-020-0126-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398915PMC
August 2020

Comparative assessment of neurotoxicity impacts induced by alkyl tri-n-butyl phosphate and aromatic tricresyl phosphate in PC12 cells.

Environ Toxicol 2020 Dec 14;35(12):1326-1333. Epub 2020 Jul 14.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China.

Organophosphate flame retardants (OPFRs) have become a growing concern due to their potential environmental and health risk. However, limited studies have described the toxicity, particularly neurotoxicity of alkyl and aromatic OPFRs. This study investigated the neurotoxicity of alkyl tri-n-butyl phosphate (TnBP) and aromatic tricresyl phosphate (TCP) to rat adrenal pheochromocytoma (PC12) cells for 24 h. Viability detection showed dose-response toxicity effect of TCP and TnBP to PC12 cells. The half-maximal inhibitory concentration of 24 h (24 h-IC ) of TCP and TnBP were 2415.61 and 338.09 μM, respectively. Both TnBP and TCP significantly changed the acetylcholinesterase (AChE) activity, and TnBP is more likely to cause neurotoxicity to PC12 cells compared to TCP. Also, The results of LDH and caspase-3 activity detection as well as Hoechst staining suggested that cell apoptosis induced by TCP and TnBP may be the primary pathway. These findings provide a toxicity data of aromatic and alkyl-substituted OPFRs to PC12 cells, and a new insight into the toxicity of OPFRs on health risk assessment.
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http://dx.doi.org/10.1002/tox.22997DOI Listing
December 2020

Acidity-Triggered Tumor-Targeted Nanosystem for Synergistic Therapy via a Cascade of ROS Generation and NO Release.

ACS Appl Mater Interfaces 2020 Jul 17;12(26):28975-28984. Epub 2020 Jun 17.

State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, 222 Tianshui Road, Lanzhou 730000, P. R. China.

Nitric oxide (NO) gas therapy has aroused intense interest in recent years. l-Arginine (l-Arg) reacts with reactive oxygen species (ROS) in tumor cells to generate NO. This phenomenon represents an effective method for tumor therapy. However, endogenous ROS levels in most types of tumor cells cannot enable an effective reaction. β-Lapachone is generally used to increase HO, which can oxidize guanidine derivatives to form nitric oxide in tumor cells. In addition, based on the ferrocene (Fc)-catalyzed Fenton reaction, ·OH is generated from HO, and the ONOO could be generated from an interaction between ·O (generated through the Haber-Weiss reaction) and NO. Arg-rich poly(ε-caprolactone) (PCL)-b-PArg, a macromolecular NO donor, was accurately synthesized to avoid premature l-Arg leakage during in vivo transport. In this design, the self-assembled PCL-b-PArg nanoparticles were dressed with the tumor-shreddable masking (PEG-b-PDMA, a negatively charged pH-sensitive hydrophilic diblock polymer), to prepare P-lapa-Fc nanoparticles and hide penetrative capability in the circulation. The experimental results confirmed that this synergistic therapy based on ROS and NO had a significant inhibitory effect on cancer cells, thereby providing new inspiration for NO gas treatment.
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http://dx.doi.org/10.1021/acsami.0c04791DOI Listing
July 2020

Diversity and Functions of Endophytic Fungi Associated with Roots and Leaves of in an Alpine Steppe at Qinghai-Tibet Plateau.

J Microbiol Biotechnol 2020 Jul;30(7):1027-1036

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R. China.

is a unique and dominant herbaceous plant species in the alpine steppe and meadows on the Qinghai-Tibet Plateau (QTP). In this work, we analyzed the composition and diversity of the culturable endophytic fungi in according to morphological and molecular identification. Then, we investigated the bioactivities of these fungi against plant pathogenic fungi and 1- aminocyclopropane-1-carboxylate deaminase (ACCD) deaminase activities. A total of 323 fungal isolates were first isolated from , and 33 fungal taxa were identified by internal transcribed spacer primers and grouped into Ascomycota. The diversity of endophytic fungi in was significantly higher in roots as compared to leaves. In addition, more than 40% of the endophytic fungi carried the gene encoding for the ACCD gene. The antibiosis assay demonstrated that 29, 35, 28, 37 and 34 isolates (43.9, 53.1, 42.4, 56.1, and 51.5%) were antagonistic to five plant pathogenic fungi, respectively. Our study provided the first assessment of the diversity of culturedepending endophytic fungi of , demonstrated the potential application of ACCD activity and antifungal activities with potential benefits to the host plant, and contributed to high biomass production and adaptation of to an adverse environment.
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http://dx.doi.org/10.4014/jmb.2002.02056DOI Listing
July 2020

Anti-aging effects on Caenorhabditis elegans of a polysaccharide, O-acetyl glucomannan, from roots of Lilium davidii var. unicolor Cotton.

Int J Biol Macromol 2020 Jul 27;155:846-852. Epub 2020 Mar 27.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address:

The anti-aging activities on Caenorhabditis elegans of a polysaccharide, O-acetyl glucomannan (LPR), purified from roots of Lilium davidii var. unicolor Cotton, were assessed by observing the mean lifespan, reproduction, pharyngeal pumping and stress response on nematodes. Additionally, the fluorescence intensity of lipofuscin and the level of reactive oxygen species (ROS) were detected. Also the activities of superoxide dismutase (SOD), catalase (CAT) and contents of malondialdehyde (MDA) were determined by the kit method. The results showed that LPR effectively delayed the aging of C. elegans in a dose-dependent manner. When the concentration reached 4 mg/mL, LPR extended the mean lifespan of C. elegans by up to 40%, 61% (P < 0.01) and 50% (P < 0.05) under normal, thermal and oxidative stress culture conditions, respectively. Moreover, LPR remarkably increased the reproduction duration of the nematodes at a concentration of 1 mg/L, and significantly decreased the ROS and lipofuscin level of C. elegans in three dosage groups. Further study illustrated that LPR at 4 mg/mL strongly increased the activity of SOD and CAT by 39.03% (P < 0.01) and 41.89% (P < 0.05), and decreased the lipid peroxidation of MDA level in C. elegans by 52.59% (P < 0.005) compared to a control. It was inferred that LPR provided stress resistance to heat and oxidation, and prolonged the lifespan of wild type N2 C. elegans mainly by elevating the function of nematode antioxidant defense systems and by scavenging free radicals. These findings provided evidence for the anti-aging properties of this polysaccharide from L. davidii.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.03.206DOI Listing
July 2020

Bronchial epithelial pyroptosis promotes airway inflammation in a murine model of toluene diisocyanate-induced asthma.

Biomed Pharmacother 2020 May 31;125:109925. Epub 2020 Jan 31.

Department of Rheumatology and Immunology, The Third Affiliated Hospital, Southern Medical University, Institute of Clinical Immunology, Academy of Orthopedics of Guangdong Province, Guangdong Provincial Key Laboratory of Bone and Joint Degenerative Diseases, Guangzhou, Guangdong, China. Electronic address:

Airway epithelial injury in response to allergens such as toluene diisocyanate (TDI) leads to persistent airway inflammation. Pyroptosis is recognized as a strong proinflammatory cell death process. However, the role of pyroptosis in bronchial epithelial injury and airway inflammation in TDI-induced asthma remains unknown. In this study, cytotoxic effect of TDI on 16HBE cells (a human bronchial epithelial cell line) was detected. Then a TDI-induced experimental asthma mouse model was established for in vivo study. Here we found that TDI induced pyroptosis in 16HBE cells, as evidenced by enhanced expressions of caspase-1 and elevated levels of LDH, IL-1β and HMGB1. As expected, TDI-induced inflammatory cell death was significantly blocked by a specific NLRP3 inflammasome inhibitor. Intriguingly, in asthmatic mice, the increased cleavages of caspase-1 and pyroptotic executioner gasdermin D (GSDMD) in bronchial epithelial cells were decreased by NLRP3 inflammasome inhibitor. Furthermore, inhibition of NLRP3 inflammasome attenuated airway hyper-responsiveness and airway inflammation, accompanied by lower levels of IL-1β, IgE and Th2-related cytokines. Our data suggest that bronchial epithelial pyroptosis exacerbates airway inflammation and hyper-responsiveness in TDI-induced asthma via NLRP3 inflammasome activation and GSDND cleavage. Therefore, NLRP3 inflammasome-mediated pyroptosis may be a potential treatment target for TDI-induced asthma.
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http://dx.doi.org/10.1016/j.biopha.2020.109925DOI Listing
May 2020

Isolable cyclic radical cations of heavy main-group elements.

Chem Commun (Camb) 2020 Feb 23;56(14):2167-2170. Epub 2020 Jan 23.

State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

One-electron oxidation of 1,3-digerma-2,4-dipnictacyclobutadiene [LGeE] (L = CH[CHNDipp], dipp = 2,6-PrCH; 1: E = P; 2: E = As) with Ag[Al(OR)] (R = C(CF)) afforded the stable radical cation salts 1˙·[Al(OR)] and 2˙·[Al(OR)], respectively. The radical cation salts have been fully characterized, in conjunction with theoretical calculations. The EPR spectroscopic studies and DFT calculations reveal that the spin density mainly resides at the heavy pnictogen atoms, rather than delocalizes over the GeE ring. They represent the first structurally characterized cyclic radical species composed of both heavy group 14 and 15 elements.
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http://dx.doi.org/10.1039/c9cc09582aDOI Listing
February 2020

Hydrocortisone Suppresses Early Paraneoplastic Inflammation And Angiogenesis To Attenuate Early Hepatocellular Carcinoma Progression In Rats.

Onco Targets Ther 2019 8;12:9481-9493. Epub 2019 Nov 8.

School of Pharmacy and Medical Sciences, and UniSA Cancer Research Institute, University of South Australia, Adelaide, SA 5001, Australia.

Background: Inflammation is implicated in both hepatic cirrhosis development and hepatocellular carcinogenesis, and treatment with long-acting glucocorticoid dexamethasone prevented liver carcinogenesis in mice. However, it is unclear whether glucocorticoids have anti-inflammatory effect on hepatocellular carcinoma (HCC) and if short-acting glucocorticoids (with fewer adverse effects) inhibit paraneoplastic inflammation and HCC progression.

Methods: To investigate whether different types of anti-inflammatory agents attenuate HCC progression, the current study compared effects of treatments with hydrocortisone (a short-acting glucocorticoid) or aspirin on HCC progression. HCC was induced in diethylnitrosamine-treated rats which were randomly divided into 4 groups (n=8), respectively receiving orally once daily vehicle, glucuronolactone, glucuronolactone+hydrocortisone, and glucuronolactone+aspirin. Diethylnitrosamine (DEN) was given to rats in drinking water (100mg/L) to induce HCC. At weeks 12 and 16 post-induction, effects were compared on HCC nodule formation, microvessel density, and macrophage infiltration, and levels of paraneoplastic protein expression of tumor necrosis factor (TNF)-α, p38 mitogen-activated protein kinase (p38), phosphorylated p38 (p-p38), nuclear factor (NF)-κB, interleukin (IL)-10, hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1 and vascular endothelial growth factor (VEGF).

Results: Compared to the model and glucuronolactone alone groups, HCC nodule number and microvessel density in the glucuronolactone+hydrocortisone group were significantly lower at week 12. At week 12 but not week 16, significantly lower levels of macrophages, TNF-α, p-p38, NF-κB, IL-10, HGF, TGF-β1 and VEGF were observed in the paraneoplastic tissue of the glucuronolactone+hydrocortisone group when compared with the control and glucuronolactone groups.

Conclusion: The results suggest that hydrocortisone treatment reduces macrophage polarization, expression of inflammatory and anti-inflammatory cytokines, and angiogenesis in paraneoplastic tissue, and attenuates early HCC progression. Although hydrocortisone did not have attenuation effect on advanced solid tumor, the current study shows the potential benefits and supports potential clinical use of hydrocortisone in attenuating early progression of HCC, which is through suppressing paraneoplastic inflammation and angiogenesis.
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http://dx.doi.org/10.2147/OTT.S224618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850701PMC
November 2019

High GILT Expression and an Active and Intact MHC Class II Antigen Presentation Pathway Are Associated with Improved Survival in Melanoma.

J Immunol 2019 11 7;203(10):2577-2587. Epub 2019 Oct 7.

Department of Basic Medical Sciences, University of Arizona College of Medicine, Phoenix, AZ 85004;

The MHC class I Ag presentation pathway in melanoma cells has a well-established role in immune-mediated destruction of tumors. However, the clinical significance of the MHC class II Ag presentation pathway in melanoma cells is less clear. In Ag-presenting cells, IFN-γ-inducible lysosomal thiol reductase (GILT) is critical for MHC class II-restricted presentation of multiple melanoma Ags. Although not expressed in benign melanocytes of nevi, GILT and MHC class II expression is induced in malignant melanocytes in a portion of melanoma specimens. Analysis of The Cancer Genome Atlas cutaneous melanoma data set showed that high GILT mRNA expression was associated with improved overall survival. Expression of IFN-γ, TNF-α, and IL-1β was positively associated with GILT expression in melanoma specimens. These cytokines were capable of inducing GILT expression in human melanoma cells in vitro. GILT protein expression in melanocytes was induced in halo nevi, which are nevi undergoing immune-mediated regression, and is consistent with the association of GILT expression with improved survival in melanoma. To explore potential mechanisms of GILT's association with patient outcome, we investigated pathways related to GILT function and expression. In contrast to healthy skin specimens, in which the MHC class II pathway was nearly uniformly expressed and intact, there was substantial variation in the MHC class II pathway in the The Cancer Genome Atlas melanoma specimens. Both an active and intact MHC class II pathway were associated with improved overall survival in melanoma. These studies support a role for GILT and the MHC class II Ag presentation pathway in melanoma outcome.
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http://dx.doi.org/10.4049/jimmunol.1900476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832889PMC
November 2019

Antagonistic effect of vitamin E on nAlO-induced exacerbation of Th2 and Th17-mediated allergic asthma via oxidative stress.

Environ Pollut 2019 Sep 26;252(Pt B):1519-1531. Epub 2019 Jun 26.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China. Electronic address:

Some basic research has shown that nanomaterials can aggravate allergic asthma. However, its potential mechanism is insufficient. Based on the research that alumina nanopowder (nAlO) has been reported to cause lung tissue damage, the purpose of this study was to explore the relationship between nAlO and allergic asthma as well as its molecular mechanism. In this study, Balb/c mice were sensitized with ovalbumin (OVA) to construct the allergic asthma model while intratracheally administered 0.5, 5 or 50 mg kg·day nAlO for 3 weeks. It was observed that exposure to nAlO exacerbated airway hyperresponsiveness (AHR), airway remodeling, and inflammation cell infiltration, leading to lung function damage in mice. Results revealed that nAlO could increase ROS levels and decrease GSH levels in lung tissue, promote the increases of the T-IgE, TGF-β, IL-1β and IL-6 levels, stimulate the overexpression of transcription factors GATA-3 and RORγt, decrease the levels of IFN-γ and IL-10 and increase the levels of IL-4 and IL-17A, resulting in the imbalance of Th1/Th2 and Treg/Th17 immune responses. In addition, antioxidant Vitamin E (Vit E) could alleviate asthma-like symptoms through blocking oxidative stress. The study displayed that exposure of nAlO deteriorated allergic asthma through promoting the imbalances of Th1/Th2 and Treg/Th17.
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http://dx.doi.org/10.1016/j.envpol.2019.06.092DOI Listing
September 2019

Tailored Design of an ROS-Responsive Drug Release Platform for Enhanced Tumor Therapy via "Sequential Induced Activation Processes".

ACS Appl Mater Interfaces 2019 Jul 9;11(29):25654-25663. Epub 2019 Jul 9.

The reactive oxygen species (ROS)-responsive intelligent drug delivery system has developed rapidly in recent years. However, because of the low concentration of ROS in most types of tumor cells, it is not possible to rapidly and effectively stimulate the drug delivery system to release the active drug. Here, we introduced "sequential induced activation processes" for efficient tumor therapy by designing a new ROS-responsive drug release platform. β-Lapachone, a positively charged nitrogen mustard (NM) prodrug, and two diblock molecules (mPEG-AcMH and PAsp-AcMH) are self-assembled to form prodrug primary micelles, which are further aggregated into nanoparticles that facilitate drug codelivery. When administered by intravenous injection, the nanoparticles reach the tumor site and enter the tumor cells by endocytosis. The β-lapachone released in the tumor cells induces a large amount of HO, and the ROS-responsive NM prodrug is activated to form activated NM, quinone methide, and boric acid under the induction of HO. The activated NM leads to tumor cell apoptosis.
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http://dx.doi.org/10.1021/acsami.9b01433DOI Listing
July 2019

Purification, characterization and antioxidant activities of a polysaccharide from the roots of Lilium davidii var. unicolor Cotton.

Int J Biol Macromol 2019 Aug 6;135:1208-1216. Epub 2019 Jun 6.

CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address:

A polysaccharide (LPR) was separated from the roots of Lilium davidii var. unicolor Cotton with hot water extraction, ethanol precipitation, and purification by anion-exchange and gel-permeation chromatography. LPR was characterized. The weight-average molecular weight (M) of LPR was 5.12 × 10 g/moL. Glucose and mannose comprised LPR with a molar ratio of 2.9:3.3. IR, NMR and methylation analysis showed that LPR was a natural O-acetyl glucomannan, the backbone mainly contained β-(1 → 4)-linked d-glucopyranosyl and β-(1 → 4)-linked D-mannopyanosyl, and the branches probably linked at O-2 and/or O-3 of the mannosyl and glucosyl residues. The acetyl groups mainly attached at O-2 or O-3 of mannosyl residues. X-ray diffractometric (XRD) analysis and scanning electron microscopy (SEM) analysis revealed that LPR was a semi-crystalline substance with porous lamellar structure. Bioassays in vitro indicated that LPR had distinct scavenging activities on hydroxyl radical and DPPH radical. These findings provided a reference for functional underutilization roots of L. davidii as natural antioxidant in food and pharmaceutical industry.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.06.030DOI Listing
August 2019

Features of the cervicovaginal microenvironment drive cancer biomarker signatures in patients across cervical carcinogenesis.

Sci Rep 2019 05 14;9(1):7333. Epub 2019 May 14.

Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona, Phoenix, AZ, USA.

Persistent human papillomavirus (HPV) infection is the vital factor driving cervical carcinogenesis; however, other features of the local cervicovaginal microenvironment (CVM) may play a critical role in development of precancerous cervical dysplasia and progression to invasive cervical carcinoma (ICC). Here we investigated relationships between locally secreted cancer biomarkers and features of the local CVM to better understand the complex interplay between host, virus and vaginal microbiota (VMB). We enrolled women with ICC, high- and low-grade squamous intraepithelial lesions, as well as, HPV-positive and healthy HPV-negative controls. A broad range of cancer biomarkers was present in the local CVM and specifically elevated in ICC patients. The majority of cancer biomarkers were positively correlated to other biomarkers and linked to genital inflammation. Several cancer biomarkers were also negatively correlated to Lactobacillus abundance and positively correlated with abnormal vaginal pH. Finally, a hierarchical clustering analysis of cancer biomarkers and immune mediators revealed three patient clusters, which varied in levels of cancer biomarkers, genital inflammation, vaginal pH and VMB composition. Specific cancer biomarkers discriminated patients with features of the CVM, such as high genital inflammation, elevated vaginal pH and dysbiotic non-Lactobacillus-dominant VMB, that have been associated with HPV persistence, dysplasia and progression to ICC.
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http://dx.doi.org/10.1038/s41598-019-43849-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517407PMC
May 2019

Dermal exposure to nano-TiO induced cardiovascular toxicity through oxidative stress, inflammation and apoptosis.

J Toxicol Sci 2019 ;44(1):35-45

Laboratory of Environmental Biomedicine, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, China.

Due to its excellent properties such as ultraviolet obscuration, chemical stability and small particle size, nano-titanium dioxide (nano-TiO) is widely used, particularly in sunblock products. The skin is therefore a chief route for exposure. Studies have found that oral or respiratory exposure to nano-TiO has an adverse impact on the cardiovascular system. The relationship between dermal exposure to nano-TiO and cardiovascular system toxicity, particularly the causative mechanisms, remain unclear. In this study, we used Balb/c mice to evaluate cardiovascular toxicity from nano-TiO dermal exposure, and the underlying mechanisms associated with the human umbilical vein endothelial cells (HUVECs) were explored. Our results showed that nano-TiO treatment resulted in an obvious increase in reactive oxygen species and 8-hydroxy-2'-deoxyguanosine, indicating the appearance of oxidative stress. Moreover, the levels of inflammatory biomarkers, such as immunoglobulin E, soluble intercellular adhesion molecule-1, interleukin-8, and hypersensitive C-reactive protein, also increased. Exposing HUVECs to nano-TiO led to a decline in cell vitality, and an increase in caspase-3 levels, suggesting that nano-TiO exposure caused cytotoxicity and even cell apoptosis. Interestingly, neutralizing oxidative stress by administering Vitamin E was shown to reduce the inflammatory response and cytotoxicity. Our findings suggest that nano-TiO can injure the cardiovascular system via dermal exposure, and does this via oxidative stress-induced inflammation and cytotoxicity. Vitamin E treatment may be a strategy to mitigate the damage.
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http://dx.doi.org/10.2131/jts.44.35DOI Listing
January 2019

Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans.

Pak J Pharm Sci 2018 Nov;31(6):2487-2495

Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China.

We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans. A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments. For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power. Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w). One fraction (FRP-2) was isolated and purified, and its characteristics were analyzed. The average mean molecular weight of FRP-2 was measured to be 2.60×10 Da, and its monosaccharide composition is mainly glucose. The sugar units are present both in the α-configuration and β-configuration. Moreover, FRP-2 exhibited certain anti-ultraviolet activity to C. elegans when the polysaccharide concentration ranged between 0.05mg/mL and 0.20mg/mL.
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November 2018

Antagonistic effect of epigallocatechin-3-gallate on neurotoxicity induced by formaldehyde.

Toxicology 2019 01 3;412:29-36. Epub 2018 Nov 3.

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China. Electronic address:

The toxicity of formaldehyde (FA) has always been of great concern, particularly since its use is unavoidable. On the other hand, epigallocatechin-3-gallate (EGCG), an active substance in tea polyphenols, has been shown to demonstrate physiological protective functions by in both epidemiological and zoological studies, particularly in the nervous system. The study described here, aims to explore whether EGCG can alleviate the neurotoxic effects induced by formaldehyde. After 14 days of exposure to 3 mg/m formaldehyde, mice exhibited significant cognitive impairment. In the FA group, a significant increase in iNOS level compared with the control group was observed. The reduced GSH level was significantly decreased. The levels of IL-1β, TNF-α and Caspase-3 were obviously raised, while H&E and Nissl staining illustrated significant neuronal damage. After administering EGCG as a protective agent, all the above observed changes were reversed, and the protective effect of EGCG became gradually evident in the 20-500 mg/kg range. Immunohistochemistry results showed that EGCG could activate the Nrf2 signaling pathway, thus alleviating the oxidative damage caused by formaldehyde.
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http://dx.doi.org/10.1016/j.tox.2018.10.022DOI Listing
January 2019

Genome-wide association study identifies two risk loci for tuberculosis in Han Chinese.

Nat Commun 2018 10 4;9(1):4072. Epub 2018 Oct 4.

Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Science and Technology, Tongji University, Shanghai, 200092, China.

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb), and remains a leading public health problem. Previous studies have identified host genetic factors that contribute to Mtb infection outcomes. However, much of the heritability in TB remains unaccounted for and additional susceptibility loci most likely exist. We perform a multistage genome-wide association study on 2949 pulmonary TB patients and 5090 healthy controls (833 cases and 1220 controls were genome-wide genotyped) from Han Chinese population. We discover two risk loci: 14q24.3 (rs12437118, P = 1.72 × 10, OR = 1.277, ESRRB) and 20p13 (rs6114027, P = 2.37 × 10, OR = 1.339, TGM6). Moreover, we determine that the rs6114027 risk allele is related to decreased TGM6 transcripts in PBMCs from pulmonary TB patients and severer pulmonary TB disease. Furthermore, we find that tgm6-deficient mice are more susceptible to Mtb infection. Our results provide new insights into the genetic etiology of TB.
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http://dx.doi.org/10.1038/s41467-018-06539-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172286PMC
October 2018

The Diradical-Dication Strategy for BODIPY- and Porphyrin-Based Dyes with Near-Infrared Absorption Maxima from 1070 to 2040 nm.

Chemistry 2018 Dec 23;24(72):19341-19347. Epub 2018 Nov 23.

State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, 210023, P. R. China.

Four stable boron dipyrromethene (BODIPY)- and porphyrin-based bis-arylamine diradical dications were synthesized by two-electron oxidation of their neutral molecules. The two BODIPY-based dications have open-shell singlet ground states. UV/Vis absorption spectra of all four dications showed large redshifts in the NIR region compared to their neutral precursors with absorption maxima at 1274 and 1068 nm for the two BODIPY-based dications and 1746 and 2037 nm for the two porphyrin-based dications. Thus, two new types of NIR dyes with longer wavelengths are provided by the diradical-dication strategy, which can be applied for the generation of other NIR dyes with a range of different chromophores and auxochromes.
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http://dx.doi.org/10.1002/chem.201804449DOI Listing
December 2018

Linking cervicovaginal immune signatures, HPV and microbiota composition in cervical carcinogenesis in non-Hispanic and Hispanic women.

Sci Rep 2018 05 15;8(1):7593. Epub 2018 May 15.

Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona, Phoenix, AZ, USA.

While high-risk human papillomavirus (HPV) infection is a well-established risk factor for cervical cancer, there are likely other factors within the local microenvironment that contribute to cervical carcinogenesis. Here we investigated relationships between HPV, vaginal pH, vaginal microbiota (VMB) composition, level of genital immune mediators and severity of cervical neoplasm. We enrolled women with low- and high-grade cervical dysplasia (LGD, HGD), invasive cervical carcinoma (ICC), and healthy controls. HPV16, HPV45, HPV58, and HPV31 were the most prevalent in our cohort with HPV16 and HPV31 genotypes more prevalent in Hispanics. Vaginal pH was associated with ethnicity and severity of cervical neoplasm. Lactobacillus dominance decreased with the severity of cervical neoplasm, which correlated with elevated vaginal pH. Hispanic ethnicity was also associated with decreased Lactobacillus dominance. Furthermore, Sneathia was enriched in all precancerous groups, ICC, abnormal pH and Hispanic origin. Patients with ICC, but not LGD and HGD, exhibited increased genital inflammatory scores and elevated specific immune mediators. Notably, IL-36γ was significantly associated with ICC. Our study revealed local, host immune and microbial signatures associated with cervical carcinogenesis and provides an initial step to understanding the complex interplay between mucosal inflammation, HPV persistence and the VMB.
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http://dx.doi.org/10.1038/s41598-018-25879-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954126PMC
May 2018