Publications by authors named "Haiping Zhang"

152 Publications

Risk factors for esophageal squamous cell carcinoma and its histological precursor lesions in China: a multicenter cross-sectional study.

BMC Cancer 2021 Sep 16;21(1):1034. Epub 2021 Sep 16.

Department of Endoscopy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Background: Despite research efforts, the causative factors that contribute to esophageal squamous cell carcinoma (ESCC) in high-risk areas have not yet been understood. In this study, we, therefore, aimed to describe the risk factors associated with ESCC and its precursor lesions.

Methods: We performed an endoscopic examination of 44,857 individuals aged 40-69 years from five high incidence regions of China in 2017-2018. Participants were classified as 4 groups of normal control, esophagitis, low-grade intraepithelial neoplasia (LGIN) and high-grade intraepithelial neoplasia/esophageal squamous cell carcinoma (HGIN/ESCC) using an unconditional logistic regression determine risk factors.

Results: We identified 4890 esophagitis, 1874 LGIN and 437 HGIN/ESCC cases. Crude odds ratios (ORs) and adjusted odds ratios were calculated using unconditional logistic regression. Drinking well and surface water, salty diet, and positive family history of cancer were the common risk factors for esophagitis, LGIN and HGIN/ESCC. History of chronic hepatitis/cirrhosis was the greatest risk factor of esophagitis (adjusted OR 2.96, 95%CI 2.52-3.47) and HGIN/ESCC (adjusted OR 1.91, 95%CI 1.03-3.22). Pesticide exposure (adjusted OR 1.20, 95%CI 1.05-1.37) was essential risk factor of LGIN.

Conclusions: Among individuals aged 40-69 years in high incidence regions of upper gastrointestinal cancer, the results provided important epidemiological evidence for the prevention of different precancerous lesions of ESCC.
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http://dx.doi.org/10.1186/s12885-021-08764-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444572PMC
September 2021

BRD7 increases resistance to EGFR tyrosine kinase inhibitor (EGFR-TKI) in non-small cell lung cancer.

Minerva Med 2021 Sep 3. Epub 2021 Sep 3.

Department of Oncology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China -

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http://dx.doi.org/10.23736/S0026-4806.21.07716-8DOI Listing
September 2021

A novel anatomic titanium mesh cage for reducing the subsidence rate after anterior cervical corpectomy: a finite element study.

Sci Rep 2021 Jul 28;11(1):15399. Epub 2021 Jul 28.

Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University College of Medicine, No. 76 Nanguo Road, Xi'an, 710054, Shaanxi, China.

Fusion with a titanium mesh cage (TMC) has become popular as a conventional method after cervical anterior corpectomy, but postoperative TMC subsidence has often been reported in the literature. We designed a novel anatomic cervical TMC to reduce the postoperative subsidence rate. According to the test process specified in the American Society of Testing Materials (ASTM) F2267 standard, three-dimensional finite element analysis was used to compare the anti-subsidence characteristics of a traditional TMC (TTMC) and novel TMC (NTMC). Through analysis, the relative propensity values of a device to subside (Kp) of the TTMC and NTMC were 665.5 N/mm and 1007.2 N/mm, respectively. A higher Kp measurement is generally expected to indicate that the device is more resistant to subsidence into a vertebral body. The results showed that the novel anatomic titanium mesh cage (NTMC) significantly improved the anti-subsidence performance after anterior cervical corpectomy and fusion (ACCF), which was approximately 51.3% higher than that of the traditional titanium mesh cage.
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http://dx.doi.org/10.1038/s41598-021-94787-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319430PMC
July 2021

Pain location is associated with fracture type in acute osteoporotic thoracolumbar vertebral fracture: a prospective observation study.

Pain Med 2021 Jul 28. Epub 2021 Jul 28.

Department of Spine Surgery, Honghui Hospital of Xi'an Jiaotong University Health Science Center.

Objective: This study investigated the relationship between pain location and fracture type in the patients with acute osteoporotic vertebral fracture (OVF).

Design: A prospective observation study.

Subject: A total of 306 patients with acute OVF were included.

Methods: The site of pain of each patient was recorded, and the patients were divided into pain at fracture site group (group 1) and pain at non-fracture site group (group 2). The fracture type was classified into four types: type I, upper endplate type; type II, central type; type III, lower endplate type; type IV, burst type.

Results: There were 146 patients in the group 1, of which 20.55% (30/146) were type I, 33.56% (49/146) were type II, 15.75% (23/146) were type III, and 30.14% (44/146) were type IV. There were 227 patients in the group 2, of which 57.27% (130/227) were type I, 5.29% (12/227) were type II, 35.24% (80/227) were type III, and 2.20% (5/227) were type IV. There was a statistical difference in the fracture type distribution between the two groups (P < 0.05). The VAS in the group 1 was higher than that in the group 2 at the initial diagnosis (P < 0.05).

Conclusions: For patients with acute OVF, the site of pain is related to the type of fracture. The pain at the fracture site is more often observed in the central type and burst type of fractures, while pain at the non-fracture site is more often observed in the upper and lower endplate type of fractures. Additionally, when OVF are suspected, radiological assessment of thoracic and lumbar spine is recommended to better detect fractures that could cause pain distal to the site of the fracture.
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http://dx.doi.org/10.1093/pm/pnab229DOI Listing
July 2021

Hemodynamics and anesthetic effect of propofol combined with remifentanil in patients undergoing laparoscopic ovarian cystectomy under laryngeal mask airway anesthesia.

Am J Transl Res 2021 15;13(5):5404-5411. Epub 2021 May 15.

Department of Anesthesiology, Sichuan Provincial Maternity and Child Health Care Health Hospital Chengdu 610045, Sichuan Province, China.

Objective: To study the effect of propofol combined with remifentanil on hemodynamics and anesthetic effect in patients undergoing laparoscopic ovarian cystectomy under laryngeal mask airway (LMA) anesthesia.

Methods: From December 2018 to December 2019, gynecological patients who underwent laparoscopic ovarian cystectomy in our hospital were chosen and randomly separated into control group (group A) and combination group (group B). In the group B, patients were anesthetized with remifentanil combined with propofol. In the group A, patients were anesthetized with fentanyl combined with propofol. The anesthetic effect, hemodynamic changes, alertness-sedation score (OAAS), verbal depiction score (VRA), postoperative VAS score and adverse reactions were observed and compared in both groups.

Results: The anesthesia induction time, recovery time of postoperative spontaneous respiration, time of opening eyes and time of removing laryngeal mask in the group B were shorter than those in the group A, and the difference was statistically significant (P<0.05). The OAAS scores at the time of recovery and 5 min after laryngeal mask removal in the group B were obviously lower than those in the group A. The mean arterial pressure and heart rate before and after 40 min pneumoperitoneum were more stable than those in the group A. The degree of postoperative pain in the group B was also significantly weaker than that in the group A. The incidence of postoperative adverse reactions was also lower than that of the group A, and the difference was statistically significant (P<0.05).

Conclusion: Propofol combined with remifentanil and LMA anesthesia has better anesthetic effect, more stable condition and higher safety for patients undergoing laparoscopic ovarian cystectomy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205666PMC
May 2021

Evaluation of residue-residue contact prediction methods: From retrospective to prospective.

PLoS Comput Biol 2021 05 24;17(5):e1009027. Epub 2021 May 24.

University of Chinese Academy of Sciences, Beijing, China.

Sequence-based residue contact prediction plays a crucial role in protein structure reconstruction. In recent years, the combination of evolutionary coupling analysis (ECA) and deep learning (DL) techniques has made tremendous progress for residue contact prediction, thus a comprehensive assessment of current methods based on a large-scale benchmark data set is very needed. In this study, we evaluate 18 contact predictors on 610 non-redundant proteins and 32 CASP13 targets according to a wide range of perspectives. The results show that different methods have different application scenarios: (1) DL methods based on multi-categories of inputs and large training sets are the best choices for low-contact-density proteins such as the intrinsically disordered ones and proteins with shallow multi-sequence alignments (MSAs). (2) With at least 5L (L is sequence length) effective sequences in the MSA, all the methods show the best performance, and methods that rely only on MSA as input can reach comparable achievements as methods that adopt multi-source inputs. (3) For top L/5 and L/2 predictions, DL methods can predict more hydrophobic interactions while ECA methods predict more salt bridges and disulfide bonds. (4) ECA methods can detect more secondary structure interactions, while DL methods can accurately excavate more contact patterns and prune isolated false positives. In general, multi-input DL methods with large training sets dominate current approaches with the best overall performance. Despite the great success of current DL methods must be stated the fact that there is still much room left for further improvement: (1) With shallow MSAs, the performance will be greatly affected. (2) Current methods show lower precisions for inter-domain compared with intra-domain contact predictions, as well as very high imbalances in precisions between intra-domains. (3) Strong prediction similarities between DL methods indicating more feature types and diversified models need to be developed. (4) The runtime of most methods can be further optimized.
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http://dx.doi.org/10.1371/journal.pcbi.1009027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177648PMC
May 2021

Overexpression of CRABP2 inhibits dexamethasone-induced apoptosis in human osteoblast cells.

J Orthop Surg Res 2021 Apr 20;16(1):272. Epub 2021 Apr 20.

Department of Endocrinology, the Affiliated Hospital of Xuzhou medical University, Xuzhou, 221000, China.

Background: The purpose of the current study was to explore the role and underlying mechanism of cellular retinoic acid binding protein 2 (CRABP2) in dexamethasone (DEX)-induced apoptosis in human osteoblast cells.

Methods: GSE10311 was downloaded from the Gene Expression Omnibus (GEO) database to identify the differentially expressed genes (DEGs) by the limma/R package. Primary human osteoblast was isolated and treated with different concentration of DEX (0, 10, 10, 10, 10, and 10 mol/L), and cell viability and flow cytometry were used to detect cell proliferation and apoptosis. A CRABP2 overexpression plasmid (oe-CRABP2) was used to overexpress CRABP2, and western blotting was conducted to detect protein expression.

Results: We found that CRABP2 was downregulated in the DEX-treated group. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that DEGs were associated with PI3K/Akt signaling pathway. DEX downregulated CRABP2 gene and protein expression, inhibited viability, and induced human osteoblast apoptosis. Overexpression of CRABP2 reversed DEX-induced apoptosis in human osteoblast. Moreover, overexpression of CRABP2 delayed the progression of DEX-induced osteonecrosis of the femoral head (ONFH) animal model.

Conclusion: In conclusion, CRABP2 is effective at inhibiting DEX-induced human osteoblast apoptosis and delayed ONFH progression.
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http://dx.doi.org/10.1186/s13018-021-02386-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059161PMC
April 2021

Value of MRI texture analysis for predicting new Gleason grade group.

Br J Radiol 2021 May 11;94(1121):20210005. Epub 2021 Mar 11.

Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Objectives: To explore the potential value of multiparametric magnetic resonance imaging (mpMRI) texture analysis (TA) to predict new Gleason Grade Group (GGG).

Methods: Fifty-eight lesions of fifty patients who underwent mpMRI scanning, including -weighted imaging (T2WI) and diffusion-weighted imaging (DWI) prior to trans-rectal ultrasound (TRUS)-guided core prostate biopsy, were retrospectively enrolled. TA parameters were obtained by the postprocessing software, and each lesion was assigned to its corresponding GGG. TA parameters derived from T2WI and DWI were statistically analyzed in detail.

Results: Energy, inertia, and correlation derived from apparent diffusion coefficient (ADC) maps and T2WI had a statistically significant difference among the five groups. Kurtosis, energy, inertia, correlation on ADC maps and Energy, inertia on T2WI were moderately related to the GGG trend. ADC-energy and T2-energy were significant independent predictors of the GGG trend. ADC-energy, T2WI-energy, and T2WI-correlation had a statistically significant difference between GGG1 and GGG2-5. ADC-energy were significant independent predictors of the GGG1. ADC-energy, T2WI-energy, and T2WI-correlation showed satisfactory diagnostic efficiency of GGG1 (area under the curve (AUC) 84.6, 74.3, and 83.5%, respectively), and ADC-energy showed excellent sensitivity and specificity (88.9 and 95.1%, respectively).

Conclusion: TA parameters ADC-energy and T2-energy played an important role in predicting GGG trend. Both ADC-energy and T2-correlation produced a high diagnostic power of GGG1, and ADC-energy was perfect predictors of GGG1.

Advances In Knowledge: TA parameters were innovatively used to predict new GGG trend, and the predictive factors of GGG1 were screen out.
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http://dx.doi.org/10.1259/bjr.20210005DOI Listing
May 2021

One-stage posterior en-bloc spondylectomy following reconstruction with individualized 3D printed artificial vertebrae for multi-segment thoracolumbar metastases: case report and literature review.

Am J Transl Res 2021 15;13(1):115-123. Epub 2021 Jan 15.

Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University College of Medicine Xi'an 710054, Shaanxi, China.

In thoracolumbar vertebral tumors, reconstruction of complex multi-segment thoracolumbar vertebrae after total en-bloc spondylectomy (TES) is still challenging. In recent years, with the development of 3D printing technology, individualized 3D printed artificial vertebrae have been attempted to reconstruct complex multi-segment thoracolumbar spine. Compared with traditional titanium mesh or bone transplantation, it helps reduce long-term complications, bringing a new dawn for reconstructing multi-segment thoracolumbar spine. A 69-year-old female complained of low back pain with limited motion for 1 month. More than 2 months ago, she underwent radical mastectomy due to breast cancer (Luminal A subtype). Imageology examination revealed an osteolytic lesion involving the T11-L1 vertebra. She was performed one-stage 3-segment (T11-L1) en-bloc spondylectomy via posterior approach, and then an artificial vertebrae produced by a novel individualized 3D printing technology was used for reconstruction. The patient was follow-up for 2 years, and she recovered well, with no tumor recurrence, and no complications after spinal reconstruction. The application of individualized 3D printed artificial vertebrae in multi-segment thoracolumbar spine reconstruction can not only reconstruct the bone defect more accurately through the individualized design, but the porous design is able to achieve biomechanical performance comparable to that of cancellous bone, and it is conducive to inducing bone growth, all of which help reduce long-term mechanical complications. Furthermore, the application of artificial vertebrae in surgery can significantly shorten the operation time, reduce intraoperative blood loss and reduce the risk of perioperative complications. Therefore, individualized 3D printed artificial vertebrae is a good choice for complex multi-segment thoracolumbar spine reconstruction.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847514PMC
January 2021

The number of lymph nodes examined is associated with survival outcomes and nodal upstaging in patients with stage I small cell lung cancer.

Surg Oncol 2021 Jun 31;37:101513. Epub 2020 Dec 31.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address:

Objective(s): Lymph node status is vital for patients with small cell lung cancer (SCLC). We sought to evaluate the association between the number of lymph nodes examined (NLNE) and prognosis and nodal upstaging in stage I SCLC patients.

Methods: We queried the Surveillance, Epidemiology and End Results (SEER) database and our department for surgically treated patients with pathologic stage I SCLC to evaluate the correlation between NLNE and overall survival (OS). We further investigated the association between the NLNE and nodal upstaging in clinical stage I SCLC.

Results: A total of 878 patients with pathologic stage I SCLC were enrolled from the SEER database. Univariate and multivariate Cox regression analysis revealed that removing more than 6 lymph nodes was associated with significantly improved OS. We validated the prognostic impact from examining more than 6 nodes in pathologic stage I SCLC patients from our department. Logistic regression analysis found that removing more than 6 nodes increased the odds of nodal upstaging for clinical stage I SCLC.

Conclusions: Adequate nodal examination leads to survival benefits and accurate nodal staging. Our analysis indicated that examining more than 6 lymph nodes could confer better OS and predict nodal upstaging for stage I SCLC patients.
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http://dx.doi.org/10.1016/j.suronc.2020.12.007DOI Listing
June 2021

Quick evaluation of lower leg ischemia in patients with peripheral arterial disease by time maximum intensity projection CT angiography: a pilot study.

BMC Med Imaging 2021 01 6;21(1). Epub 2021 Jan 6.

Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The purpose of this study is to evaluate a new method involving time maximum intensity projection (t-MIP) postprocessed from dynamic computed tomographic angiography (dyn-CTA) in diagnosing peripheral arterial disease (PAD).

Methods: A population of 34 patients with known PAD was examined with a combined CTA protocol consisting of a standard CTA (s-CTA) scan of the lower extremities and a dyn-CTA scan of the calves. For each lower leg, t-MIP images consisting of the MIP (sagittal MIP), MIP (45° lateral MIP), and MIP (- 45° lateral MIP) were automatically generated from dyn-CTA. An objective evaluation of the vascular CT attenuation of the best enhancement phase of dyn-CTA and t-MIP was measured; a subjective evaluation of vessel stenosis and occlusion was performed, assigning a score for t-MIP and s-CTA. The CT attenuation of t-MIP and dyn-CTA was compared, as were the runoff scores of t-MIP and s-CTA.

Results: The CT attenuation of t-MIP CTA of three vascular segments from 68 lower extremities was higher than that of the best enhancement phase of dyn-CTA and s-CTA, with statistically significant differences at the posterior tibial artery and fibular artery (all p < 0.05). There were strong correlations (r ≥ 0.75, p < 0.05) of the runoff scores between t-MIP and s-CTA.

Conclusions: There is potential clinical applicability of t-MIP in assisting with the diagnosis of lower leg vascular stenosis in dyn-CTA with reliable diagnostic accuracy and convenient immediacy.
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http://dx.doi.org/10.1186/s12880-020-00537-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789570PMC
January 2021

A novel virtual screening procedure identifies Pralatrexate as inhibitor of SARS-CoV-2 RdRp and it reduces viral replication in vitro.

PLoS Comput Biol 2020 12 31;16(12):e1008489. Epub 2020 Dec 31.

Center for High Performance Computing, Joint Engineering Research Center for Health Big Data Intelligent Analysis Technology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China.

The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus poses serious threats to the global public health and leads to worldwide crisis. No effective drug or vaccine is readily available. The viral RNA-dependent RNA polymerase (RdRp) is a promising therapeutic target. A hybrid drug screening procedure was proposed and applied to identify potential drug candidates targeting RdRp from 1906 approved drugs. Among the four selected market available drug candidates, Pralatrexate and Azithromycin were confirmed to effectively inhibit SARS-CoV-2 replication in vitro with EC50 values of 0.008μM and 9.453 μM, respectively. For the first time, our study discovered that Pralatrexate is able to potently inhibit SARS-CoV-2 replication with a stronger inhibitory activity than Remdesivir within the same experimental conditions. The paper demonstrates the feasibility of fast and accurate anti-viral drug screening for inhibitors of SARS-CoV-2 and provides potential therapeutic agents against COVID-19.
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http://dx.doi.org/10.1371/journal.pcbi.1008489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774833PMC
December 2020

A novel bone cement screw system combined with vertebroplasty for the treatment of Kummell disease with bone deficiency at the vertebral anterior border: A minimum 3-year follow-up study.

Clin Neurol Neurosurg 2021 02 17;201:106434. Epub 2020 Dec 17.

Department of Orthopedics, The Frist Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China; Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China. Electronic address:

Objectives: When vertebroplasty is used to treat Kummell disease with bone deficiency at the vertebral anterior border, bone cement displacement often occurs intraoperatively or postoperatively. We designed and used a new bone cement screw system to avoid this serious complication. The purpose of this study was to evaluate the safety and effectiveness of this novel operation method through more than 3 years of follow-up.

Patients And Methods: From January 2014 to August 2016, 27 patients suffering from single-segment Kummell disease with bone deficiency at the vertebral anterior border were treated by vertebroplasty combined with a novel bone cement screw. Bone cement was released into the diseased vertebrae through the screw to fully fill the intravertebral vacuum cleft. Screw fixation of bone cement can avoid intraoperatively or postoperatively displacement. All patients received surgery involving a unilateral technique, and only one screw was implanted in each patient. The clinical efficacy was evaluated using Odom's criteria and statistical analysis of the vertebral body index (VBI), vertebral body angle (VBA), bisegmental Cobb angle (BCA), visual analogue scale (VAS), Oswestry disability index (ODI), and the results of the MOS 36-item short form health survey (SF-36).

Results: The operation was completed successfully in 27 cases. The average operation time was 49.63 ± 10.82 min, and the average volume of cement injected was 4.70 ± 0.87 mL. The patients' preoperative VBI, VBA, BCA, VAS and ODI scores were 43.11 ± 5.94, 21.04 ± 2.55, 45.00 ± 6.26, 7.59 ± 0.84, and 79.85 ± 7.58, respectively. The postoperative measurements were 78.70 ± 2.55, 12.70 ± 2.11, 26.11 ± 4.73, 3.22 ± 0.93 and 50.04 ± 9.28, respectively. At the last follow-up, the measurements were 78.04 ± 2.30, 13.15 ± 2.38, 27.07 ± 4.87, 2.04 ± 0.65, and 22.85 ± 5.06, respectively. There was a significant difference between the preoperative and postoperative data, as well as the preoperative and the last follow-up data (P < 0.05). Comparing the results of SF-36 before operation and at the last follow-up revealed significant differences in physical function, role-physical, body pain, vitality, and social function (P < 0.05). However, there were no significant differences in general health, emotional function and mental health. Finally, 26 patients (96.3 %) had good to excellent clinical outcomes according to Odom's criteria.

Conclusions: This 3-year follow-up study shows that the novel bone cement screw system combined with vertebroplasty has a good short and medium-term therapeutic effect in patients with Kummell disease and bone deficiency at the vertebral anterior border, while its long-term efficacy is subject to further studies.
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http://dx.doi.org/10.1016/j.clineuro.2020.106434DOI Listing
February 2021

Salivary microbial changes during the first 6 months of orthodontic treatment.

PeerJ 2020 1;8:e10446. Epub 2020 Dec 1.

Department of Preventive Dentistry, School of Stomatology, Capital Medical University, Beijing, China.

Background: Orthodontic treatment is widely used to treat malocclusion. However, the influence of treatment on the oral microbiome remains unclear. In this study, we investigated salivary microbial changes in patients undergoing orthodontic treatment.

Methods: In total, 19 orthodontic patients participated in this study. Saliva samples were collected at the following three timepoints: before (T0) and 3 months (T1) and 6 months (T2) after the placement of orthodontic appliances. High-throughput sequencing was performed based on the 16S rRNA gene V4 region.

Results: The phyla of Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Fusobacteria were predominant. Observed Species, Chao1 and ACE, which represent diversity, were significantly decreased at T1 and subsequently increased at T2. In addition, the diversity at T1 based on the Bray-Curtis distances differed from T0 and T2. The relative abundances of , and were decreased with treatment, whereas those of and exhibited the opposite results. In total, 385 of 410 operational taxonomic units were shared at T0, T1 and T2. The co-occurrence networks with hub nodes at T1 were the most complex.

Conclusion: Orthodontic treatment temporarily affected the saliva microbial community. This dynamic alteration in species did not induce deterioration in oral health. Oral hygiene instructions were necessary and should be emphasized during each visit. Further studies with longer observation periods and more participants are required.
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http://dx.doi.org/10.7717/peerj.10446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718796PMC
December 2020

Simultaneous detection of fetal aneuploidy, de novo FGFR3 mutations and paternally derived β-thalassemia by a novel method of noninvasive prenatal testing.

Prenat Diagn 2021 03 21;41(4):440-448. Epub 2021 Jan 21.

BGI-Shenzhen, Shenzhen, China.

Objective: The aim is to develop a novel noninvasive prenatal testing (NIPT) method that simultaneously performs fetal aneuploidy screening and the detection of de novo and paternally derived mutations.

Methods: A total of 68 pregnancies, including 26 normal pregnancies, 7 cases with fetal aneuploidies, 7 cases with fetal achondroplasia or thanatophoric dysplasia, 18 cases with fetal skeletal abnormalities, and 10 cases with β-thalassemia high risk were recruited. Plasma cell-free DNA was amplified by Targeted And Genome-wide simultaneous sequencing (TAGs-seq) to generate around 99% of total reads covering the whole-genome region and around 1%  covering the target genes. The reads on the whole-genome region were analyzed for fetal aneuploidy using a binary hypothesis T-score and the reads on target genes were analyzed for point mutations by calculating the minor allelic frequency of loci on FGFR3 and HBB. TAGs-seq results were compared with conventional NIPT and diagnostic results.

Results: In each sample, TAGs-seq generated 44.7-54 million sequencing reads covering the whole-genome region of 0.1-3× and the target genes of >1000×depth. All cases of fetal aneuploidy and de novo mutations of achondroplasia/thanatophoric dysplasia were identified with high sensitivities and specificities except for one false-negative paternal mutation of β-thalassemia.

Conclusions: TAGs-seq is a novel NIPT method that combines the fetal aneuploidy screening and the detection of de novo FGFR3 mutations and paternal HBB mutations.
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http://dx.doi.org/10.1002/pd.5879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048498PMC
March 2021

Psychodermatology knowledge and awareness in Chinese dermatologists: Results of a survey study.

Dermatol Ther 2021 01 5;34(1):e14668. Epub 2021 Jan 5.

Department of Dermatology, Xuanwu Hospital, Capital Medical University, Beijing, China.

To assess the knowledge, awareness, practice patterns, and attitudes of Chinese dermatologists toward psychocutaneous disorders and explore their interest in continuing medical education (CME) of psychodermatology. An online survey study was conducted from October to November 2019. The survey questionnaire link was sent to the members of the national and local association of dermatology through a social media App. After confirming the informed consent to enrol in the study, the participants filled out the online questionnaire anonymously. About 1047 dermatologists completed the survey. The most common diagnoses referred to the psychiatrist from Chinese dermatologists were venereal phobia (51.77%), delusion of parasitosis (44.03%), and trichotillomania (32.28%). Compared with dermatologists, Traditional Chinese Medicine (TCM) dermatologists had less training experience on psychodermatologic CME (4.21% vs 8.34% who had attending more than twice CME, P < .05). Although TCM dermatologist experience more frequency with psychodermatology (24.21% TCM vs 15.71% dermatologists), they had lower comfort level in treating psychodermatologic patients (51.58% TCM vs 58.89% dermatologist) (P > .05). While 818 (78.13%) dermatologists never received any training course, 84.53% of the dermatologists expressed interest in attending CME events on psychodermatology. The most popular CME themes of psychodermatology were emotional disorders related to skin diseases, delusion of parasitosis, and trichotillomania. Chinese dermatologists have insufficient knowledge and awareness toward psychodermatology. TCM dermatologists have higher awareness on psychocutaneous diseases compared with dermatologists. Psychodermatology continuing medical education programs should be carried out as soon as possible.
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http://dx.doi.org/10.1111/dth.14668DOI Listing
January 2021

Identification and Validation of New Stable QTLs for Grain Weight and Size by Multiple Mapping Models in Common Wheat.

Front Genet 2020 11;11:584859. Epub 2020 Nov 11.

KeyLaboratory of Wheat Biology and Genetic Improvement on Southern Yellow and Huai River Valley, Ministry of Agriculture and Rural Affairs, College of Agronomy, Anhui Agricultural University, Hefei, China.

Improvement of grain weight and size is an important objective for high-yield wheat breeding. In this study, 174 recombinant inbred lines (RILs) derived from the cross between Jing 411 and Hongmangchun 21 were used to construct a high-density genetic map by specific locus amplified fragment sequencing (SLAF-seq). Three mapping methods, including inclusive composite interval mapping (ICIM), genome-wide composite interval mapping (GCIM), and a mixed linear model performed with forward-backward stepwise (NWIM), were used to identify QTLs for thousand grain weight (TGW), grain width (GW), and grain length (GL). In total, we identified 30, 15, and 18 putative QTLs for TGW, GW, and GL that explain 1.1-33.9%, 3.1%-34.2%, and 1.7%-22.8% of the phenotypic variances, respectively. Among these, 19 (63.3%) QTLs for TGW, 10 (66.7%) for GW, and 7 (38.9%) for GL were consistent with those identified by genome-wide association analysis in 192 wheat varieties. Five new stable QTLs, including 3 for TGW (, , and ) and 2 for GL ( and ), were detected by the three aforementioned mapping methods across environments. Subsequently, five cleaved amplified polymorphic sequence (CAPS) markers corresponding to these QTLs were developed and validated in 180 Chinese mini-core wheat accessions. In addition, 19 potential candidate genes for in a 0.31-Mb physical interval were further annotated, of which and encode a plasma membrane H-ATPase and a serine/threonine-protein kinase, respectively. These new QTLs and CAPS markers will be useful for further marker-assisted selection and map-based cloning of target genes.
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http://dx.doi.org/10.3389/fgene.2020.584859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686802PMC
November 2020

Development of a novel silver ions-nanosilver complementary composite as antimicrobial additive for powder coating.

Chem Eng J 2021 Sep 6;420:127633. Epub 2020 Nov 6.

Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Applying silver into coatings has become a prevalent method in fabricating antimicrobial surfaces. However, the concerns about durability always exist and limit its applications. Here, a highly inhibitory, active, durable, and easy-to-use silver ions-nanosilver antimicrobial additive for powder coatings was fabricated in this study. Silver nanoparticles were chemically bonded to the Ag, Cu, and Zn-ternary ion-exchanged zeolite by -lipoic acid, which was then encapsulated by hydrophilic polymers. The fabricated silver ions and silver nanoparticles (Ag-AgNPs) complementary structure provides a synergistic effect. Ag is the main antimicrobial agent, while AgNPs act as a supplementary reservoir of Ag. As well, the formed thin layer of silver nanoparticles and hydrophilic film prolongs the release of active Ag from zeolite, and Ag facilitates the activation of AgNPs. The results show that this additive indicates excellent antimicrobial activity to , , , and , and that the coatings with the additive exhibit over 99.99% reduction rate for the tested bacteria and fungi. The coating film is able to maintain over 99% antimicrobial reduction even after 1200 repeated solution wipings, or over 30 wash cycles of artificial sweat solution, indicating high durability. Furthermore, the yellowness of the coating is not evident (Δb < 2) despite the high loading of silver, and the silver nanoparticles have little impact on gloss, haze, and distinctness of the coating film image.
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http://dx.doi.org/10.1016/j.cej.2020.127633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644439PMC
September 2021

Natural Potent NAAA Inhibitor Atractylodin Counteracts LPS-Induced Microglial Activation.

Front Pharmacol 2020 2;11:577319. Epub 2020 Oct 2.

Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, School of Medicine, Xiamen University, Xiamen, China.

-acylethanolamine-hydrolyzing acid amidase (NAAA) is a lysosomal enzyme that inhibits the degradation of palmitoylethanolamide (PEA), an endogenous lipid that induces analgesic, anti-inflammation, and anti-multiple sclerosis through PPARα activation. Only a few potent NAAA inhibitors have been reported to date, which is mainly due to the restricted substrate-binding site of NAAA. Here, we established a high-throughput fluorescence-based assay for NAAA inhibitor screening. Several new classes of NAAA inhibitors were discovered from a small library of natural products. One of these is atractylodin, a polyethylene alkyne compound from the root of (Thunb) DC., which significantly inhibits NAAA activity and has an IC of 2.81 µM. Kinetic analyses and dialysis assays suggested that atractylodin engages in competitive inhibition reversible reaction to the enzyme. Docking assays revealed that atractylodin occupies the catalytic cavity of NAAA, where the atractylodin furan head group has a hydrophobic-related interaction with the backbone of the Trp181 and Leu152 residues of human NAAA. Further investigation indicated that atractylodin significantly increases PEA and OEA levels and dose-dependently inhibits LPS-induced nitrate, TNF-α, IL-1β, and IL-6 pro-inflammatory cytokine release in BV-2 microglia. Our results show that atractylodin elevates cellular PEA levels and inhibits microglial activation by inhibiting NAAA activity, which in turn could contribute to NAAA functional research.
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http://dx.doi.org/10.3389/fphar.2020.577319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565389PMC
October 2020

Structural basis for the inhibition of SARS-CoV2 main protease by Indian medicinal plant-derived antiviral compounds.

J Biomol Struct Dyn 2020 Oct 19:1-9. Epub 2020 Oct 19.

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamilnadu, India.

A novel coronavirus (SARS-CoV2) has caused a major outbreak in humans around the globe, and it became a severe threat to human healthcare than all other infectious diseases. Researchers were urged to discover and test various approaches to control and prevent such a deadly disease. Considering the emergency and necessity, we screened reported antiviral compounds present in the traditional Indian medicinal plants for the inhibition of SARS-CoV2 main protease. In this study, we used molecular docking to screen 41 reported antiviral compounds that exist in Indian medicinal plants and shown amentoflavone from the plant with a higher docking score. Furthermore, we performed a 40 ns atomic molecular dynamics simulation and free binding energy calculations to explore the stability of the top five protein-ligand complexes. Through the article, we insist that the amentoflavone, hypericin and Torvoside H from the traditional Indian medicinal plants may be used as a potential inhibitor of SARS-CoV2 main protease and further biochemical experiments could shed light on understanding the mechanism of inhibition by these plant-derived antiviral compounds.Communicated by Ramaswamy H. Sarma.
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http://dx.doi.org/10.1080/07391102.2020.1834457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594188PMC
October 2020

Hepatocellular Carcinoma With Different Areas of Right Retroperitoneal Space Invasion: Evaluation of Transcatheter Arterial Chemoembolization Efficacy and Blood Supply Characteristics.

Front Oncol 2020 23;10:539692. Epub 2020 Sep 23.

Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: To evaluate the therapeutic efficacy of transcatheter arterial chemoembolization (TACE) for hepatocellular carcinoma (HCC) with different areas of right retroperitoneal space (rRPS) invasion and analyze the blood supply.

Methods: This retrospective study enrolled 41 patients with HCC with different areas of rRPS invasion treated with TACE, including 22 HCCs with superior aspect of the right perirenal space (SARPS) invasion and 19 HCCs with right anterior pararenal space (RAPS) invasion. The overall response rate (ORR) and disease control rate (DCR) were analyzed. The prognostic factors for overall survival (OS) after TACE were determined. The blood supply characteristics of HCC with different areas of rRPS invasion were analyzed with arteriograms.

Results: All patients underwent 2.8 ± 1.8 TACE sessions over 25.0 ± 21.9 months. The median OS was 29.0 months for patients with SARPS invasion and 12.0 months for patients with RAPS invasion ( = 0.004). Only the invaded area of the rRPS was an independent prognostic factor for OS [hazard ratio (HR), 2.833; 95% CI, 1.297-6.188; and = 0.009). The ORR and DCR were significantly higher in the group with SARPS invasion than in the group with RAPS invasion (ORR: 63.6% vs 31.6%, = 0.041; DCR: 77.3% vs 47.4%, = 0.047). Initially, HCC with SARPS invasion were supplied by the hepatic artery (HA; = 8) and both the HA and extrahepatic collateral vessels (EHCs; = 14); HCC with RAPS invasion were supplied by the HA ( = 10) and both the HA and EHCs ( = 9); as the TACE sessions increased, the tumor-feeding vessels shifted from the HA to both the HA and EHCs, and even EHCs could be the only blood supply. Rare EHCs appeared earlier and more frequently in the RAPS group than in the SARPS group.

Conclusion: The efficacy of TACE differed for HCC with different areas of rRPS invasion, and the median OS, ORR and DCR were significantly better in the SARPS group than in the RAPS group. Different common EHCs supplied HCCs with different areas of rRPS invasion, while other rare EHCs appeared more frequently in the RAPS group.
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http://dx.doi.org/10.3389/fonc.2020.539692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538699PMC
September 2020

Identification of the wheat C3H gene family and expression analysis of candidates associated with seed dormancy and germination.

Plant Physiol Biochem 2020 Nov 7;156:524-537. Epub 2020 Oct 7.

College of Agronomy, Anhui Agricultural University, Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, Ministry of Agriculture and Rural Affairs, Hefei, 230036, Anhui, China.

C3H zinc finger transcription factors play important roles in managing various biotic/abiotic stresses in Aarabidopsis, rice, and maize. The functions of these factors in wheat, however, remain largely unclear. We identified 88 TaC3H genes that were divided into four subfamilies in this analysis. Gene structure and conserved domain analyses indicate that most members of the same subfamily have similar structures. A total of 76 paralogous and 48 orthologous pairs were identified and Ka/Ks values were used to analyze replication relationships amongst wheat, rice, and Arabidopsis. Gene ontology (GO) annotation analysis showed that most TaC3H genes possessed molecular functions, while transcriptome results showed that the 88 TaC3H genes responded to water imbibition. Microarray data for 53 TaC3H genes were obtained and heat maps were generated; these results indicate that these genes are expressed in 13 wheat tissues. Subcellular localization prediction analysis indicates that most TaC3H genes are located in the nucleus. Promoter analysis indicates that most TaC3H genes contained cis-elements including ABRE, GARE-motif, and MBS, indicating that these can respond to various biotic/abiotic stresses. Transcriptome data and quantitative real-time PCR analysis of wheat cultivars with contrasting seed dormancy phenotypes show that five genes TaC3H4/-18/-37/-51/-72 were very likely involved in seed dormancy and germination. Exogenous ABA treatment further indicated that these five genes were responsive to ABA, suggesting that there may be a crosstalk between these genes and ABA signaling pathway in controlling seed dormancy and germination. These results provide a theoretical basis for subsequent studies on TaC3H gene function and also contribute to studies on the C3H gene in other species.
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http://dx.doi.org/10.1016/j.plaphy.2020.09.032DOI Listing
November 2020

Rational combination therapy for hepatocellular carcinoma with PARP1 and DNA-PK inhibitors.

Proc Natl Acad Sci U S A 2020 10 5;117(42):26356-26365. Epub 2020 Oct 5.

Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China;

Understanding differences in DNA double-strand break (DSB) repair between tumor and normal tissues would provide a rationale for developing DNA repair-targeted cancer therapy. Here, using knock-in mouse models for measuring the efficiency of two DSB repair pathways, homologous recombination (HR) and nonhomologous end-joining (NHEJ), we demonstrated that both pathways are up-regulated in hepatocellular carcinoma (HCC) compared with adjacent normal tissues due to altered expression of DNA repair factors, including PARP1 and DNA-PKcs. Surprisingly, inhibiting PARP1 with olaparib abrogated HR repair in HCC. Mechanistically, inhibiting PARP1 suppressed the clearance of nucleosomes at DNA damage sites by blocking the recruitment of ALC1 to DSB sites, thereby inhibiting RPA2 and RAD51 recruitment. Importantly, combining olaparib with NU7441, a DNA-PKcs inhibitor that blocks NHEJ in HCC, synergistically suppressed HCC growth in both mice and HCC patient-derived-xenograft models. Our results suggest the combined inhibition of both HR and NHEJ as a potential therapy for HCC.
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http://dx.doi.org/10.1073/pnas.2002917117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584895PMC
October 2020

Lymph Node Examination for Stage I Second Primary Lung Cancer Patients Who Received Second Surgical Treatment.

Ann Surg Oncol 2021 Mar 5;28(3):1810-1818. Epub 2020 Sep 5.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Purpose: This study aims to investigate the effect of lymph node examination on overall survival (OS) and lung cancer-specific survival (LCSS) in stage I second primary lung cancer (SPLC) patients who underwent second pulmonary resection.

Patients And Methods: We conducted a retrospective study with the Surveillance, Epidemiology, and End Results (SEER) database to identify stage I SPLC patients who received surgery from 1998 to 2015. The Kaplan-Meier method with landmark analysis and multivariable Cox regression analysis were performed to evaluate the prognostic value of lymph node examination.

Results: A total of 842 patients from the SEER database with stage I SPLC who underwent a second surgical treatment were included. The 5-year survival rate was 54.8% for the whole cohort. Multivariable analysis revealed that the number of lymph nodes examined (LNE) was associated with better OS and LCSS in SPLC patients after 12 months postoperatively. Patients with contralateral SPLC had significantly more nodes removed than those with ipsilateral SPLC. For contralateral SPLC, more than 10 LNE was correlated with improved long-term survival outcomes. Ipsilateral SPLC patients benefited from 4 or more LNE. However, the current analysis did not show a significant survival benefit from lymph node examination within 12 months after surgery.

Conclusions: For stage I SPLC patients who received surgical treatment after initial resection, an adequate number of LNE would improve both OS and LCSS. We recommend more than 10 LNE for contralateral SPLC and at least 4 LNE for ipsilateral SPLC.
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http://dx.doi.org/10.1245/s10434-020-08975-9DOI Listing
March 2021

Pseudogene Promotes Cell Proliferation and Migration of Hepatocellular Carcinoma by Sponging MiR-490-5p to Induce BUB1 Expression.

Front Genet 2020 22;11:666. Epub 2020 Jul 22.

Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, China.

Hepatocellular Carcinoma (HCC) currently remains one of the most lethal malignancies worldwide. Recently, long non-coding RNAs (lncRNAs) had been demonstrated to play a crucial role in the progression of multiple human cancers, including HCC. In this study, we found that lncRNA DUXAP8 was upregulated in tumor samples and served as an oncogene in HCC. Bioinformatics analysis showed that DUXAP8 was significantly associated with the regulation of centrosome organization, homologous recombination, meiotic cell cycle process, sister chromatid segregation, nuclear chromosome segregation, and RNA export from the nucleus. The knockdown of DUXAP8 significantly suppresses cell proliferation and the cell cycle but induces cell apoptosis in HCC. Mechanically, the present study showed that DUXAP8 serves as a sponge of MiR-490-5p to promote the expression of BUB1 in HCC. Although the underlying regulatory mechanisms of DUXAP8 in HCC require further investigation, this study, for the first time, showed that DUXAP8 can serve as a new therapeutic target for HCC.
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http://dx.doi.org/10.3389/fgene.2020.00666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396656PMC
July 2020

Upregulation of Orai1 and increased calcium entry contribute to angiotensin II-induced human coronary smooth muscle cell proliferation: Running Title: Angiotensin II-induced human coronary smooth muscle cells proliferation.

Peptides 2020 11 19;133:170386. Epub 2020 Aug 19.

Department of Cardiology, 920th Hospital of the PLA Joint Logistics Support Force, Kunming, Yunnan, 650032, China. Electronic address:

Angiotensin II (Ang II) is an oligopeptide of the renin-angiotensin system, and Ang II-induced vascular smooth muscle cell (VSMC) proliferation is an important pathophysiological process involved in atherosclerosis; however, the underlying mechanism remains unclear. Orai1 and Stim1 are the main components of store-operated Ca entry (SOCE), which has an important effect on VSMC proliferation. In the present study, we showed that Ang II-induced human coronary smooth muscle cell (HCSMC) proliferation was associated with increased calcium entry. The expression of Orai1, but not that of Stim1, was significantly upregulated in Ang II-treated HCSMCs. However, knockdown of Orai1 or Stim1 decreased HCSMC proliferation and SOCE activity in Ang II-treated HCSMCs. Orai1 was significantly downregulated in HCSMCs transfected with short interfering RNA (siRNA) against NOX2 or NF-κB. Transfection with siRNA against NOX2 or p65 also decreased Ang II-induced HCSMCs SOCE activation and proliferation. These findings suggested that Ang II upregulated Orai1 via the NF-κB and NOX2 pathways, leading to increased SOCE and HCSMC proliferation. The molecular factors mediating Ang II-induced SOCE upregulation are potential therapeutic targets for the prevention of Ang II-sensitive or Ang II-dependent HCSMC proliferation.
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http://dx.doi.org/10.1016/j.peptides.2020.170386DOI Listing
November 2020

Clinical characteristics of hyperprogressive disease in NSCLC after treatment with immune checkpoint inhibitor: a systematic review and meta-analysis.

BMC Cancer 2020 Jul 29;20(1):707. Epub 2020 Jul 29.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, No. 507 Zhengmin Road, Shanghai, 200433, China.

Background: A number of studies have reported hyperprogressive disease (HPD) in non-small cell lung cancer (NSCLC) after treatment with immune checkpoint inhibitor (ICI). This study aimed to summarize the incidence and survival outcome of HPD in NSCLC and identify the clinicopathological features associated with HPD based on available eligible studies.

Methods: Four databases (Medline/PubMed, Embase, Web of Science, and Cochrane Library) were searched for eligible studies on HPD published before January 23, 2020, to evaluate the incidence, outcome, and clinical features of HPD. Statistical analyses were performed using STATA 15.0. All meta-analyses were performed based on the random-effects model.

Results: This study included 6 studies involving 1389 patients. The incidence of HPD ranged from 8.02 to 30.43%. Compared with patients with non-HPD, those with HPD were associated with worse overall survival. We identified that Eastern Cooperative Oncology Group > 1, Royal Marsden Hospital score ≥ 2, serum lactate dehydrogenase > upper limit of normal, the number of metastasis sites > 2, and liver metastasis were associated with the risk of HPD.

Conclusions: This study summarized the clinical features of HPD in NSCLC patients. The meta-analysis showed that five pre-treatment clinicopathological features might be associated with HPD, which may help in selecting patients for ICIs.
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http://dx.doi.org/10.1186/s12885-020-07206-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392646PMC
July 2020

Enantioselective fate of mandipropamid in grape and during processing of grape wine.

Environ Sci Pollut Res Int 2020 Nov 13;27(32):40148-40155. Epub 2020 Jul 13.

Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China.

Enantioselective monitoring of chiral fungicide mandipropamid enantiomers were carried out in grapes and wine-making process. The enantiomers of mandipropamid were separated on a Lux Cellulose-2 column and determined by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The processing procedure included washing, fermentation, and clarification. Significant enantioselectivity was observed in grape under field conditions and during wine-making processing. The half-lives of R-mandipropamid and S-mandipropamid were 5.63 days and 7.79 days under field conditions 43.3 h and 69.3 h during wine-making processing, respectively. The EF values ranged from 0.498 to 0.283 in grape under field conditions, and the EF values were from 0.458 (0 h) to 0.362 (312 h) during the whole fermentation process. The results indicated that R-mandipropamid degraded faster than S-mandipropamid in grape under field conditions and during the fermentation process. The processing factors (PFs) were less than 1 for each procedure, and the PF ranged from 0.005 to 0.025 in the overall process, which indicated that the wine-making process can reduce mandipropamid residue in grape wine. The results of this study could help facilitate more accurate risk assessments of mandipropamid in table grapes and during wine-making process.
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http://dx.doi.org/10.1007/s11356-020-10061-2DOI Listing
November 2020

On the Conformational Dynamics of β-Amyloid Forming Peptides: A Computational Perspective.

Front Bioeng Biotechnol 2020 3;8:532. Epub 2020 Jun 3.

Center for High Performance Computing, Joint Engineering Research Center for Health Big Data Intelligent Analysis Technology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Understanding the conformational dynamics of proteins and peptides involved in important functions is still a difficult task in computational structural biology. Because such conformational transitions in β-amyloid (Aβ) forming peptides play a crucial role in many neurological disorders, researchers from different scientific fields have been trying to address issues related to the folding of Aβ forming peptides together. Many theoretical models have been proposed in the recent years for studying Aβ peptides using mathematical, physicochemical, and molecular dynamics simulation, and machine learning approaches. In this article, we have comprehensively reviewed the developmental advances in the theoretical models for Aβ peptide folding and interactions, particularly in the context of neurological disorders. Furthermore, we have extensively reviewed the advances in molecular dynamics simulation as a tool used for studying the conversions between polymorphic amyloid forms and applications of using machine learning approaches in predicting Aβ peptides and aggregation-prone regions in proteins. We have also provided details on the theoretical advances in the study of Aβ peptides, which would enhance our understanding of these peptides at the molecular level and eventually lead to the development of targeted therapies for certain acute neurological disorders such as Alzheimer's disease in the future.
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http://dx.doi.org/10.3389/fbioe.2020.00532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325929PMC
June 2020

The Relationship Between Diabetes Mellitus and COVID-19 Prognosis: A Retrospective Cohort Study in Wuhan, China.

Am J Med 2021 01 10;134(1):e6-e14. Epub 2020 Jul 10.

Department of Gastroenterology/Hepatology, Zhongnan Hospital of Wuhan University, Wuhan, P.R. of China; The Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, P.R. of China. Electronic address:

Background: Coronavirus disease 2019 (COVID-19) is an emerging infectious disease that first appeared in Wuhan, China, and quickly spread throughout the world. We aimed to understand the relationship between diabetes mellitus and the prognosis of COVID-19.

Methods: Demographic, clinical, laboratory, radiologic, treatments, complications, and clinical outcomes data were extracted from electronic medical records and compared between diabetes (n = 84) and nondiabetes (n = 500) groups. Kaplan-Meier method and multivariate Cox analysis were applied to determine the risk factors for the prognosis of COVID-19.

Results: Compared with nondiabetic patients, diabetic patients had higher levels of neutrophils (P = .014), C-reactive protein (P = .008), procalcitonin (P < .01), and D-dimer (P = .033), and lower levels of lymphocytes (P = .032) and albumin (P = .035). Furthermore, diabetic patients had a significantly higher incidence of bilateral pneumonia (86.9%, P = .020). In terms of complications and clinical outcomes, the incidence of respiratory failure (36.9% vs 24.2%, P = .022), acute cardiac injury (47.4% vs 21.2%, P < .01), and death (20.2% vs 8.0%, P = .001) in the diabetes group was significantly higher than that in the nondiabetes group. Kaplan-Meier survival curve showed that COVID-19 patients with diabetes had a shorter overall survival time. Multivariate Cox analysis indicated that diabetes (hazard ratio 2.180, P = .031) was an independent risk factor for COVID-19 prognosis. In subgroup analysis, we divided diabetic patients into insulin-required and non-insulin-required groups according to whether they needed insulin, and found that diabetic patients requiring insulin may have a higher risk of disease progression and worse prognosis after the infection of severe acute respiratory syndrome coronavirus 2.

Conclusions: Diabetes is an independent risk factor for the prognosis of COVID-19. More attention should be paid to the prevention and treatment for diabetic patients, especially those who require insulin therapy.
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http://dx.doi.org/10.1016/j.amjmed.2020.05.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350644PMC
January 2021
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