Publications by authors named "Haiping Wu"

96 Publications

Development of a simple and sensitive HPLC-DAD method for quantification of vitamin B12 fortified in infant food.

Anal Methods 2021 Oct 5. Epub 2021 Oct 5.

Research and Development Department, GRA (Shanghai) Standard Technology Service Co., Ltd, Shanghai 201318, P. R. China.

To prevent infants from vitamin B12 deficiency, infant food is designed based on cow's milk or cereal with the fortification of vitamin B12. A method for quantitative determination of vitamin B12 in infant food was developed with hydrophilic high performance liquid chromatography (HPLC) coupled with a diode array detector (DAD). The sensitivity of the detector was enhanced by implementing a 60 mm high-sensitivity LightPipe flow cell, and the limit of detection (LOD) and limit of quantification (LOQ) were improved as low as 0.006 μg 100 g and 0.02 μg 100 g respectively. The effect of sample extraction and enrichment, chromatography separation parameters on the analyte, were studied in detail and optimized. Under these conditions, the method performed a good linear analytical range of 0.3-50 μg L, and a good repeatability with % RSD below 2.8% and recovery of 90.2-96.5% ( = 6). To the best of our knowledge, for the first time, 60 mm high-sensitivity LightPipe flow cell was included in the HPLC-DAD method for determination of the trace amount of vitamin B12 in infant food. The proposed method was further validated by analysis of FAPAS QC samples (T21120 and T21118), and it was specific and precise for the intended use.
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http://dx.doi.org/10.1039/d1ay01118aDOI Listing
October 2021

Reversible capturing and voltammetric determination of circulating tumor cells using two-dimensional nanozyme based on PdMo decorated with gold nanoparticles and aptamer.

Mikrochim Acta 2021 Sep 3;188(10):319. Epub 2021 Sep 3.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.

A novel cytosensor was constructed for the ultrasensitive detection and nondestructive release of circulating tumor cells (CTCs) by combining Au nanoparticles-loaded two-dimensional bimetallic PdMo (2D [email protected]) nanozymes and electrochemical reductive desorption. The 2D [email protected] nanozymes possessed high-efficiency peroxidase-like activity and were assembled with an aptamer composed of a thiol-modified epithelial specific cell adhesion molecule (EpCAM) to strengthen CTCs adhesion. Moreover, the electrode surface was decorated with highly fractal Au nanostructures (HFAuNSs) composites due to the similarity in fractal nanostructure with the CTCs membrane to enhance the CTCs anchoring efficiency and release capability. The captured CTCs could be further efficiently dissociated and nondestructively released from the modified electrodes upon electrochemical reductive desorption. The designed cytosensor showed an excellent analytical performance, with a wide linear range from 2 to 1 × 10 cells mL and low limit of detection (LOD) of 2 cells mL (S/N = 3) at the working potential in the range  -0.6 to 0.2 V. A satisfactory CTCs release reaching a range of 93.7-97.4% with acceptable RSD from 3.55 to 6.41% and good cell viability was obtained. Thus, the developed cytosensor might provide a potential alternative to perform CTC-based liquid biopsies, with promising applications in early diagnosis of tumors. Preparation and mechanism of desorption of the cytosensor based on 2D [email protected] nanozymes and electrochemical reductive desorption for the detection and release of CTCs. A Preparation procedure of the Apt/[email protected] bioconjugates. B Fabrication process of the sandwich-type cytosensor. C Electrochemical signal produced by the [email protected] nanozymes. D Mechanism of electrochemical reductive desorption for CTCs release.
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http://dx.doi.org/10.1007/s00604-021-04927-6DOI Listing
September 2021

Combining electrochemical nitrate reduction and anammox for treatment of nitrate-rich wastewater: A short review.

Sci Total Environ 2021 Aug 11;800:149645. Epub 2021 Aug 11.

Civil & Environmental Engineering Dept., University of Nebraska-Lincoln, Omaha, NE 68182-0178, USA.

Treatment of nitrate-rich wastewater is important but challenging for the conventional biological denitrification process. Here, we propose combining the electrochemical reduction and anaerobic ammonium oxidation (anammox) processes together for treatment of nitrate-rich wastewater. This article reviews the mechanism and current research status of electrochemical reduction of nitrate to ammonium as well as the mechanism and applicability of the anammox process. This article discusses the principles, superiorities and challenges of this combined process. The feasibility of the combined process depends on the efficiency of electrochemical nitrate reduction to ammonium and the conditions in the anammox process to use the reduced ammonium as the substrate to achieve deep nitrogen removal. The article provides a feasible strategy for using the electrochemical reduction and anammox combined process to treat nitrate-rich wastewater.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149645DOI Listing
August 2021

Multi-function PtCo nanozymes/CdS [email protected] oxide luminophores and KSO/HO coreactants-based dual amplified electrochemiluminescence immunosensor for ultrasensitive detection of anti-myeloperoxidase antibody.

J Nanobiotechnology 2021 Jul 29;19(1):225. Epub 2021 Jul 29.

Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, 310014, China.

Background: Anti-myeloperoxidase antibody (anti-MPO) is an important biomarker for anti-neutrophil cytoplasm antibody (ANCA)-associated vasculitides (AAVs). However, the complicated operation procedures and insufficient sensitivity of conventional anti-MPO detection methods limit their application in monitoring efficacy of AAVs in clinical diagnosis. Herein, a dual amplified electrochemiluminescence (ECL) immunosensor based on multi-function PtCo nanozymes/CdS [email protected] oxide (PtCo/[email protected]) luminophores and KSO/HO coreactants has been fabricated for ultrasensitive detection of anti-MPO.

Results: PtCo/[email protected] luminophores as novel signal amplification labels and nanocarriers to load rabbit anti-mouse IgG were synthesized by co-doping with Pt and Co nanozymes simultaneously with several considerable advantages, including astonishing peroxidase-like catalytic activity, high-efficiency luminescence performance and superior stability in aqueous solutions. Meanwhile, upon the KSO/HO coreactants system, benefiting from the efficient peroxidase-like activity of the PtCo/[email protected] toward HO, massive of transient reactive intermediates could react with KSO, thus obtaining higher ECL emission. Therefore, the developed ECL immunosensor for anti-MPO detection displayed good analytical performance with good concentration linearity in the range of 0.02 to 1000 pg/mL and low detection limit down to 7.39 fg/mL.

Conclusions: The introduction of multi-function PtCo/[email protected] luminophores into the established ECL immunoassay not only was successfully applied for specific detection of anti-MPO in clinical serum samples, but also provided a completely new concept to design other high-performance luminophores. Meaningfully, the ECL immunoassay strategy held wide potential for biomarkers detection in clinical diagnosis.
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http://dx.doi.org/10.1186/s12951-021-00968-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323290PMC
July 2021

One-Pot Identification of BCR/ABL Transcript Isoforms Based on Nanocluster Beacon.

ACS Sens 2021 08 29;6(8):2928-2937. Epub 2021 Jul 29.

The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

The BCR/ABL fusion gene is a classic biomarker of chronic myeloid leukemia, which can be divided into e13a2 and e14a2 isoforms according to different breakpoints. These two isoforms showed distinct differences in clinical manifestation, treatment effect, and prognosis risk. Herein, a strategy based on nanocluster beacon (NCB) fluorescence was developed to identify the e13a2 and e14a2 isoforms in one-pot. Because the fluorescence of AgNCs can be activated when they are placed in proximity to the corresponding enhancer sequences, thymine-rich (T-rich) or guanine-rich (G-rich). In this work, we explored an ideal DNA-AgNCs template as an excellent molecular reporter with a high signal-to-noise ratio. After recognition with the corresponding isoforms, the AgNCs can be pulled closer to the T-rich or G-rich sequences to form a three-way junction structure and generate fluorescence with corresponding wavelengths. Therefore, by distinguishing the corresponding wavelengths of AgNCs, we successfully identified two isoforms in one tube with the limitation of 16 pM for e13a2 and 9 pM for e14a2. Moreover, this strategy also realized isoform identification in leukemia cells and newly diagnosed CML patients within 40 min, which provides a powerful tool to distinguish fusion gene subtypes at the same time.
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http://dx.doi.org/10.1021/acssensors.1c00695DOI Listing
August 2021

Flap Endonuclease 1-Assisted DNA Walkers for Sensitively and Specifically Sensing ctDNAs.

Anal Chem 2021 07 30;93(27):9593-9601. Epub 2021 Jun 30.

Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.

DNA walkers have shown superior performance in biosensing due to their programmability to design molecular walking behaviors with specific responses to different biological targets. However, it is still challenging to make DNA walkers capable of distinguishing DNA targets with single-base differences, so that DNA walkers that can be used for circulating tumor DNA sensing are rarely reported. Herein, a flap endonuclease 1 (FEN 1)-assisted DNA walker has been proposed to achieve mutant biosensing. The target DNA is captured on a gold nanoparticle (AuNP) as a walking strand to walk by hybridizing to the track strands on the surface of the AuNP. FEN 1 is employed to report the walking events by cleaving the track strands that must form a three-base overlapping structure recognized by FEN 1 after hybridizing with the captured target DNA. Owing to the high specificity of FEN 1 for structure recognition, the one-base mutant DNA target can be discriminated from wild-type DNA. By constructing a sensitivity-enhanced DNA walker system, as low as 1 fM DNA targets and 0.1% mutation abundance can be sensed, and the theoretical detection limits for detecting the DNA target and mutation abundance achieve 0.22 fM and 0.01%, respectively. The results of epidermal growth factor receptor (EGFR) L858R mutation detection on cell-free DNA samples from 15 patients with nonsmall cell lung cancer were completely consistent with that of next-generation sequencing, indicating that our DNA walker has potential for liquid biopsy.
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http://dx.doi.org/10.1021/acs.analchem.1c01765DOI Listing
July 2021

An "off-on" electrochemiluminescence biosensor coupled with strand displacement-powered 3D micromolecule walking nanomachine for ultrasensitive detection of adenosine triphosphate.

Mikrochim Acta 2021 06 28;188(7):237. Epub 2021 Jun 28.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.

A three-dimensional (3D) micromolecule walking nanomachine propelled by strand displacement was developed to establish a novel switching electrochemiluminescence (ECL) biosensor for ultrasensitive detection of adenosine triphosphate (ATP). Generally, walking nanomachines reported previously were limited to DNA walkers, while the proposed 3D walking nanomachine focused on the micromolecule walker. Firstly, TiO and silver nanoparticles (Ag NPs) functionalized N-(4-aminobutyl)-N-(ethylisoluminol) (Ag-ABEI) were deposited onto the electrode surface to offer an enhanced ECL signal, resulting from the double catalytic effect of TiO and Ag NPs for HO. Following, dopamine (DA)-labeled DNA duplex probes (S1/S2-DA) immobilized onto the modified electrode cut down the original ECL signal due to the quenching of DA toward ABEI (signal-off). Target ATP walker moved along the 3D DNA track, simultaneously releasing numerous DNA3, which was applied to displace S2-DA, resulting in the quenched ECL intensity recovery (signal-on). As a result, the biosensor showed a low limit of detection down to 0.5 nM (S/N = 3) and was successfully employed to determine ATP in human serum samples. Thus, the established biosensing strategy holds great potential for biochemical studies and clinical diagnosis.
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http://dx.doi.org/10.1007/s00604-021-04895-xDOI Listing
June 2021

A Polymer-in-Salt Electrolyte with Enhanced Oxidative Stability for Lithium Metal Polymer Batteries.

ACS Appl Mater Interfaces 2021 Jul 25;13(27):31583-31593. Epub 2021 Jun 25.

Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.

The lithium (Li) metal polymer battery (LMPB) is a promising candidate for solid-state batteries with high safety. However, high voltage stability of such a battery has been hindered by the use of polyethylene oxide (PEO), which oxidizes at a potential lower than 4 V versus Li. Herein, we adopt the polymer-in-salt electrolyte (PISE) strategy to circumvent the disadvantage of the PEO-lithium bis(fluorosulfonyl)imide (LiFSI) system with EO/Li ≤ 8 through a dry ball-milling process to avoid the contamination of the residual solvent. The obtained solid-state PISEs exhibit distinctly different morphologies and coordination structures which lead to significant improvement in oxidative stability. P(EO)LiFSI has a low melting temperature, a high ionic conductivity at 60 °C, and an oxidative stability of ∼4.5 V versus Li/Li. With an effective interphase rich in inorganic species and a good stability of the hybrid polymer electrolyte toward Li metal, the LMPB constructed with Li||LiNiCoMnO can retain 74.4% of capacity after 186 cycles at 60 °C under the cutoff charge voltage of 4.3 V. The findings offer a promising pathway toward high-voltage stable polymer electrolytes for high-energy-density and safe LMPBs.
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http://dx.doi.org/10.1021/acsami.1c04637DOI Listing
July 2021

Optimization of Magnesium-Doped Lithium Metal Anode for High Performance Lithium Metal Batteries through Modeling and Experiment.

Angew Chem Int Ed Engl 2021 Jul 21;60(30):16506-16513. Epub 2021 Jun 21.

Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.

Lithium (Li)-magnesium (Mg) alloy with limited Mg amount, which can also be called Mg-doped Li (Li-Mg), has been considered as a potential alternative anode for high energy density rechargeable Li metal batteries. However, the optimum doping-content of Mg in Li-Mg anode and the mechanism of the improved performance are not well understood. Herein, density functional theory (DFT) calculations are used to investigate the effect of Mg amount in Li-Mg anode. The Li-Mg with about 5 wt. % Mg (abbreviated as Li-Mg5) has the lowest absorption energy of Li, thus all the surface area can be "controlled" by Mg atoms, leading to the smooth and continuous deposition of Li on the surface around the Mg center. A localized high concentration electrolyte enables Li-Mg5 to exhibit the best cycling stability in Li metal batteries with high-loading cathode and lean electrolyte under 4.4 V high-voltage, which is approaching the demand of practical application. This electrolyte also helps generate an inorganic-rich solid electrolyte interphase, which leads to smooth, compact and less corrosion layer on the Li-Mg5 surface. Both theoretical simulations and experimental results prove that Li-Mg5 has optimum Mg content and gives best battery cycling performance.
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http://dx.doi.org/10.1002/anie.202103344DOI Listing
July 2021

Metabolic scaling: individual versus intraspecific scaling of Nile tilapia (Oreochromis niloticus).

J Comp Physiol B 2021 07 2;191(4):721-729. Epub 2021 May 2.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Ocean College, Beibu Gulf University, Qinzhou, China.

We examined intraspecific scaling of the resting metabolic rate (RMR) of Nile tilapia (Oreochromis niloticus) under different culture conditions and further explored the allometric relationships between organ mass (heart, liver, brain, gills, viscera, and red muscles) and blood parameters (erythrocyte size and red blood cell counts) and body mass. Oreochromis niloticus were bred in individual and group cultures. The scaling exponent of the RMR in the individual cultures was b = 0.620-0.821 (n = 30) and that in the group culture was b = 0.770 [natural logarithm (ln) RMR = 0.770 ln M - 1.107 (n = 76)]. The results of the two experimental methods were similar and were not significantly different from 0.75 (3/4), as predicted by the metabolic theory of ecology. The active and inactive organs were scaled with body mass by an exponent of 0.940 and 1.012, respectively. There was no significant relationship between the blood parameters and body mass. These results suggest that the differences in the culture methods may not have affected the allometric scaling of O. niloticus metabolism. The proportion of active and inactive organs contributed to allometric changes in the metabolic rate with body mass. Red blood cells in fish are not generally representative, and cell size can only partially explain the allometric scaling of metabolism.
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http://dx.doi.org/10.1007/s00360-021-01376-8DOI Listing
July 2021

Multiplex detection of blood-borne pathogens on a self-driven microfluidic chip using loop-mediated isothermal amplification.

Anal Bioanal Chem 2021 May 13;413(11):2923-2931. Epub 2021 Mar 13.

Department of Clinical Pharmacy, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, Jiangsu, China.

Detection of blood-borne pathogens such as hepatitis C virus (HCV), hepatitis B virus (HBV) and human immunodeficiency virus (HIV) is essential to ensure the safety of blood transfusion. However, traditional PCR-based pathogen nucleic acid detection methods require relatively high experimental facilities and are difficult to apply in areas with limited resources. In this study, a self-driven microfluidic chip was designed to carry out multiplex detection of HBV, HCV and HIV by using loop-mediated isothermal amplification (LAMP). Benefitting from the air permeability of the polydimethylsiloxane material, the chip could accomplish sample loading within 12 min driven by the pressure difference between the reaction chambers and vacuum chambers in the chip without using pumps or any injection devices. Multiplex detection is achieved by presetting LAMP primers specific to different targets in different reaction chambers. Calcein was used as an indicator to indicate the positive amplification reaction, and the result can be recorded by a smartphone camera. After 50 min of isothermal amplification at 63 °C, 2 copies/μL of HBV, HCV and HIV target nucleic acids could be detected. The results of HBV detection of 20 clinical plasma samples by using the chip are consistent with that of the qPCR-based kit, indicating that the LAMP-based self-driven chip has the clinical application potential for blood-borne pathogen detection, especially in resource-limited areas.
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http://dx.doi.org/10.1007/s00216-021-03224-8DOI Listing
May 2021

The complete mitochondrial genome of (Jordan & Snyder, 1901).

Mitochondrial DNA B Resour 2020 Aug 12;5(3):3154-3156. Epub 2020 Aug 12.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, China.

is one of the species in the China-Vietnam Collective Fishery Zone, which has a few relevant studies. In this study, the mitochondrial genome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 17,784 bp was 32.45% for A, 25.76% for T, 15.72% for G, 26.08% for C, and its GC content was 41.8%. The mitochondrial circular genome was composed of 13 protein-coding genes, 22 transfer RNAs, 2D-loop, and 2 ribosomal RNAs. Polygenetic analysis showed that the was very close to . It can provide data reference for the analysis of genetic evolution of this species.
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http://dx.doi.org/10.1080/23802359.2020.1806130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782292PMC
August 2020

Surface plasmon resonance imaging-based biosensor for multiplex and ultrasensitive detection of NSCLC-associated exosomal miRNAs using DNA programmed heterostructure of Au-on-Ag.

Biosens Bioelectron 2021 Mar 22;175:112835. Epub 2020 Nov 22.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China. Electronic address:

Exosomal miRNAs are potential tumor biomarkers for early diagnosis of non-small cell lung cancer (NSCLC). Herein, a surface plasmon resonance imaging (SPRi)-based biosensor was developed for simultaneous detection of multiplex NSCLC-associated exosomal miRNAs in a clinical sample using Au-on-Ag heterostructure and DNA tetrahedral framework (DTF). Exosomal miRNAs are captured by various DTF probes immobilized on the gold array chip. Subsequently, single-stranded DNA (ssDNA) functionalized silver nanocube (AgNC) hybridizes with the captured exosomal miRNAs and then the ssDNA-coated Au nanoparticles assembled on the surface of AgNC, forming Au-on-Ag heterostructures as essential labels to realize amplified SPR response. With the aid of DNA programmed Au-on-Ag heterostructure and DTF, the SPRi-based biosensor exhibits wide detection range from 2 fM to 20 nM, ultralow limit of detection of 1.68 fM, enhanced capture efficiency, and improved antifouling capability. Furthermore, the biosensor enables accurate discrimination of NSCLC patients based on detection results of exosomal miRNAs. Overall, this developed biosensor is a promising tool for multiplex exosomal miRNAs detection, providing a new possibility for early diagnosis of NSCLC.
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http://dx.doi.org/10.1016/j.bios.2020.112835DOI Listing
March 2021

Role of inner solvation sheath within salt-solvent complexes in tailoring electrode/electrolyte interphases for lithium metal batteries.

Proc Natl Acad Sci U S A 2020 Nov 3;117(46):28603-28613. Epub 2020 Nov 3.

Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA 99354;

Functional electrolyte is the key to stabilize the highly reductive lithium (Li) metal anode and the high-voltage cathode for long-life, high-energy-density rechargeable Li metal batteries (LMBs). However, fundamental mechanisms on the interactions between reactive electrodes and electrolytes are still not well understood. Recently localized high-concentration electrolytes (LHCEs) are emerging as a promising electrolyte design strategy for LMBs. Here, we use LHCEs as an ideal platform to investigate the fundamental correlation between the reactive characteristics of the inner solvation sheath on electrode surfaces due to their unique solvation structures. The effects of a series of LHCEs with model electrolyte solvents (carbonate, sulfone, phosphate, and ether) on the stability of high-voltage LMBs are systematically studied. The stabilities of electrodes in different LHCEs indicate the intrinsic synergistic effects between the salt and the solvent when they coexist on electrode surfaces. Experimental and theoretical analyses reveal an intriguing general rule that the strong interactions between the salt and the solvent in the inner solvation sheath promote their intermolecular proton/charge transfer reactions, which dictates the properties of the electrode/electrolyte interphases and thus the battery performances.
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http://dx.doi.org/10.1073/pnas.2010852117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682554PMC
November 2020

A new electrochemical method for simultaneous removal of Mnand NH-N in wastewater with Cu plate as cathode.

Ecotoxicol Environ Saf 2020 Dec 24;206:111341. Epub 2020 Sep 24.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.

In this study, a new electrochemical method was used to simultaneously efficient removal of Mn and NH-N in wastewater with Cu plate as cathode. The effects of various reaction parameters on the concentrations of Mn, NH-N and by-products (NO-N and NO-N, free chlorine and residual chlorine), as well as the removal mechanism were investigated. The results showed that the removal efficiencies of Mn and NH-N were 99.1% and 92.9%, and the concentrations of NO-N, NO-N, free chlorine and residue chlorine were 0.73 mg/L, 0.15 mg/L, 0.13 mg/L and 0.63 mg/L reacting for 3 h at room temperature, respectively, when the current density was 10 mA/cm, the mass ratio of ClO and Cl was 1:1, the initial pH was 9. The concentrations of Mn, NH-N and by-products in wastewater met the integrated wastewater discharge standard (GB8978-1996). In addition, spherical manganese oxide was deposited on the anode plate, and spherical manganese oxide collapsed over electrolysis time. Manganese was mainly removed in the form of MnO, Mn(OH) and MnO. NH-N was mainly oxidized to N. Economic evalution revealed that the treatment cost was 2.93 $/m.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111341DOI Listing
December 2020

Novel Protease-Free Long-Lasting Chemiluminescence System Based on the Dox-ABEI Chimeric Magnetic DNA Hydrogel for Ultrasensitive Immunoassay.

ACS Appl Mater Interfaces 2020 Oct 7;12(42):47270-47277. Epub 2020 Oct 7.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.

Most of chemiluminescence (CL) substrates exhibit the flash-type light emission. Therefore, the long-lasting CL system is always the crown in the field of CL-based analysis methodology. In this work, we constructed a Dox-ABEI chimeric magnetic DNA hydrogel (MDH) as a novel protease-free long-lasting CL reaction system. The functional MDH can transform flash-type ABEI/HO/CO reaction into a glow-type CL system because of its block effect on delaying the diffusion rate of co-reactants, making the CL reaction gradually occur. More importantly, the functional MDH possessed the advantages of biocompatibility and controllability and could be well-designed to incorporate different biosensing strategies. Subsequently, we established a functional MDH-based long-lasting CL immunoassay system for ultrasensitive and highly specific detection of d-dimer and fibrin degradation products (FDPs). The designed CL immunoassay can detect d-dimer and FDP down to 53.7 and 31.6 fg/mL, respectively, with a wide line ranging from 100 fg/mL to 100 ng/mL, which was superior to the previously reported CL biosensing strategies. Moreover, benefiting from the magnetic separation of MDH and excellent CL performance, the developed immunoassaying method was successfully applied in the detection of clinical samples, which showed a close correlation with clinical reference technology. Thus, this functional MDH proved to be an excellent long-lasting CL system and a potential technical platform for clinical bioanalysis applications.
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http://dx.doi.org/10.1021/acsami.0c14188DOI Listing
October 2020

Molybdenum [email protected] biosensor for unamplified specific fragment detection in long nucleic acids based on magnetic composite probe-actuated deblocking of secondary structure.

Anal Methods 2020 10;12(39):4813-4822

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.

Secondary structures in long circulating tumor nucleic acids have potential obstacles for specific location point hybridized detection of gene fragments. The exploration of biosensing strategies requires selectively changing the nucleic acids conformation and inducing signal switching. Herein, a self-assembled magnetic composite probe (MCP) was fabricated by the hybridization reaction of Linker DNA and a "Y"-junction-DNA nanostructure on the surface of magnetic beads, contributing to the capture, secondary structure unlocking, and enrichment of the PML/RARα DNA "L" subtype. Then, by integrating the MCP-actuated reactor, a one-step "off-on" signal switching [email protected] biosensing method was developed for the efficient detection of the PML/RARα DNA "L" subtype. The proposed biosensor was capable of detecting 100 bases PML/RARα DNA "L" subtype with a wide linear range of 1 pM to 200 nM and a limit of detection down to 0.223 pM without signal amplification. In addition, the biosensing method was successfully applied for the detection of target in serum samples. It is worth pointing out that this simple biosensing strategy could enable long nucleic acids fragments with secondary structures from ctDNA and ctRNA to be quantitatively assayed based on direct hybridization.
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http://dx.doi.org/10.1039/d0ay01398fDOI Listing
October 2020

A low cost of phosphate-based binder for Mn and NH-N simultaneous stabilization in electrolytic manganese residue.

Ecotoxicol Environ Saf 2020 Dec 17;205:111317. Epub 2020 Sep 17.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.

Electrolytic manganese residue (EMR) is a solid waste remained in filters after using sulfuric acid to leaching manganese carbonate ore. EMR contains high concentration of soluble manganese (Mn) and ammonia nitrogen (NH-N), which seriously pollutes the environment. In this study, a low cost of phosphate based binder for Mn and NH-N stabilization in EMR by low grade-MgO (LG-MgO) and superphosphate was studied. The effects of different types of stabilizing agent on the concentrations of NH-N and Mn, the pH of the EMR leaching solution, stabilizing mechanisms of NH-N and Mn, leaching test and economic analysis were investigated. The results shown that the pH of the EMR leaching solution was 8.07, and the concentration of Mn was 1.58 mg/L, both of which met the integrated wastewater discharge standard (GB8978-1996), as well as the concentration of NH-N decreased from 523.46 mg/L to 32 mg/L, when 4.5 wt.% LG-MgO and 8 wt.% superphosphate dosage were simultaneously used for the stabilization of EMR for 50 d Mn and NH-N were mainly stabilized by Mn(PO)·2HO, MnOOH, MnO, Mn(HPO)·2HO and NHMgPO·6HO. Economic evaluation revealed that the treatment cost of EMR was $ 11.89/t. This study provides a low-cost materials for NH-N and Mn stabilization in EMR.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111317DOI Listing
December 2020

Electrochemical biosensor for ultrasensitive exosomal miRNA analysis by cascade primer exchange reaction and [email protected]@MOF nanozyme.

Biosens Bioelectron 2020 Nov 25;168:112554. Epub 2020 Aug 25.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China. Electronic address:

Exosomal miRNAs have been discovered as important and reliable biomarkers for early diagnosis of tumors. However, it is still challenging to achieve accurate determination of trace exosomal miRNAs in real samples. Herein, we report an electrochemical strategy based on the cascade primer exchange reaction (PER) with [email protected]@MOF nanozyme for ultrasensitive detection of exosomal miRNA. Target-triggered PER that only includes a gated hairpin, a primer, and DNA polymerase can produce a long single strand in an autonomous and isothermal manner. Then, the nascent strand releases the protector B used to blockade capture probes, resulting in the binding of the nanozyme to sensing interface. Under the catalysis of the nanozyme, hydrogen peroxide (HO) is decomposed into HO and O, thus producing an amplified electrochemical signal. Benefiting from the cascade PER and the noticeable catalytic activity of the multiple-layered nanozyme, the established biosensor shows high sensitivity with limit of detection down to 0.29 fM and high specificity that can distinguish homologous miRNAs with single base mismatch. The developed strategy allows discrimination of tumor cells and breast cancer patients by detecting exosomal miRNA-21, and the results of this biosensor are consistent with qRT-PCR. Therefore, the electrochemical strategy has wide potential in the early screening of tumors.
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http://dx.doi.org/10.1016/j.bios.2020.112554DOI Listing
November 2020

Current Density Regulated Atomic to Nanoscale Process on Li Deposition and Solid Electrolyte Interphase Revealed by Cryogenic Transmission Electron Microscopy.

ACS Nano 2020 Jul 6;14(7):8766-8775. Epub 2020 Jul 6.

Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.

Current density has been perceived to play a critical rule in controlling Li deposition morphology and solid electrolyte interphase (SEI). However, the atomic level mechanism of the effect of current density on Li deposition and the SEI remains unclear. Here based on cryogenic transmission electron microscopy (TEM) imaging combined with energy dispersive X-ray spectroscopy (EDS) and electron energy loss spectroscopy (EELS) electronic structure analyses, we reveal the atomic level correlation of Li deposition morphology and SEI with current density. We discover that increasing current density leads to increased overpotential for Li nucleation and growth, leading to the transition from growth-limited to nucleation-limited mode for Li dendrites. Independent of current density, the electrochemically deposited Li metal (EDLi) exhibits crystalline whisker-like morphology. The SEI formed at low current density (0.1 mA cm) is monolithic amorphous; while, a current density of above 2 mA cm leads to a mosaic structured SEI, featuring an amorphous matrix with LiO and LiF dispersoids, and the thickness of the SEI increases with the increase of current density. Specifically, the LiO particles are spatially located at the top surface of the SEI, while LiF is spatially adjacent to the Li-SEI interface. These results offer possible ways of regulating crucial microstructural and chemical features of EDLi and SEI through altering deposit conditions and consequently direct correlation with battery performance.
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http://dx.doi.org/10.1021/acsnano.0c03344DOI Listing
July 2020

An integrated electrochemical biosensor based on target-triggered strand displacement amplification and "four-way" DNA junction towards ultrasensitive detection of PIK3CA gene mutation.

Biosens Bioelectron 2020 Feb 6;150:111954. Epub 2019 Dec 6.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China. Electronic address:

A novel electrochemical biosensor was constructed for specific and ultrasensitive detection of PIK3CA gene mutation based on NsbI restriction enzyme-mediated strand displacement amplification (NsbI-SDA) and four-way DNA junction for the first time. In this biosensor, the NsbI restriction enzyme combined with strand displacement amplification (SDA) was able to specifically distinguish PIK3CA gene mutation and increase the number of DNA copies to improve electrochemical response. In the presence of target mutation gene, DNA fragments produced by the cleavage event of NsbI restriction enzyme could trigger the SDA reaction to generate massive linker chains. When the linker chains were captured on the electrode, the four-way DNA junction was then attached at the end of linker chain. By integrating electroactive molecules of methylene blue (MB) into four-way DNA junction, this sandwich-like electrochemical biosensor was able to determine the specific distinction of target mutation gene with a low detection limit of 0.001%. Finally, this strategy could be used to analyze mutation gene spiked into human serum samples, indicating the potential application in genetic analysis and clinical disease diagnosis.
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http://dx.doi.org/10.1016/j.bios.2019.111954DOI Listing
February 2020

Atomic to Nanoscale Origin of Vinylene Carbonate Enhanced Cycling Stability of Lithium Metal Anode Revealed by Cryo-Transmission Electron Microscopy.

Nano Lett 2020 Jan 11;20(1):418-425. Epub 2019 Dec 11.

Fujian Institute of Research on the Structure of Matter , Chinese Academy of Sciences , Fuzhou , Fujian 350002 , P.R. China.

Batteries using lithium (Li) metal as the anode are considered promising energy storage systems because of their high specific energy densities. The crucial bottlenecks for Li metal anode are Li dendrites growth and side reactions with electrolyte inducing safety concern, low Coulombic efficiency (CE), and short cycle life. Vinylene carbonate (VC), as an effective electrolyte additive in Li-ion batteries, has been noticed to significantly enhance the CE, whereas the origin of such an additive remains unclear. Here we use cryogenic transmission electron microscopy imaging combing with energy dispersive X-ray spectroscopy elemental and electron energy loss spectroscopy electronic structure analyses to reveal the role of the VC additive. We discovered that the electrochemically deposited Li metal (EDLi) in the VC-containing electrolyte is slightly oxidized with the solid electrolyte interphase (SEI) being a nanoscale mosaic-like structure comprised of organic species, LiO and LiCO, whereas the EDLi formed in the VC-free electrolyte is featured by a combination of fully oxidized Li with LiO SEI layer and pure Li metal with multilayer nanostructured SEI. These results highlight the possible tuning of crucial structural and chemical features of EDLi and SEI through additives and consequently direct correlation with electrochemical performance, providing valuable guidelines to rational selection, design, and synthesis of additives for new battery chemistries.
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http://dx.doi.org/10.1021/acs.nanolett.9b04111DOI Listing
January 2020

Effects of oral florfenicol on intestinal structure, function and microbiota in mice.

Arch Microbiol 2020 Jan 23;202(1):161-169. Epub 2019 Sep 23.

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, China.

Many kinds of antibiotics have effects on intestinal structure and function. In the current experimental study, we evaluate the effect of oral florfenicol on intestinal barrier in mice. Thirty adult male mice were randomly divided into two groups, the group none (N) and the group florfenicol (F), the mice in group F were orally administered florfenicol 100 mg/kg body weight (BW) for 7 days. At day 8, mice were euthanized and sampled for the analysis of alterations in genes and proteins from jejunum, jejunum morphology and microbiota analysis. Administration of florfenicol caused higher liver index (P < 0.05). In the jejunum, mucosa injury and villus rupture, compared with the group N, the villus length and V/C (villus length/crypt depth) in group F were marked decrease (P < 0.01). The transcription level of Muc2 and occludin in group F were significantly lower than those in group N (P < 0.01 or P < 0.05). The expression of APRIL, IL-17, IL-22, BAFF and sIgA on protein level were significantly down-regulated (P < 0.01 or P < 0.05), while the expression of IL-10, TGF-β, IL-6, IL-4 were significantly up-regulated (P < 0.01) in group F. The abundances of bacteria in Firmicutes and Lactobacillus decreased significantly (P < 0.01) in group F. Our results indicated that oral administration of florfenicol might have a negative impact on functions of intestinal mucosal barrier, immune system and the intestinal microbiota.
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http://dx.doi.org/10.1007/s00203-019-01731-yDOI Listing
January 2020

The complete mitochondrial genome of .

Mitochondrial DNA B Resour 2019 Sep 11;4(2):2942-2943. Epub 2019 Sep 11.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, China.

is a common and important component of mangrove ecosystem. In this study, the mitogenome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 15,710 bp was 31.37% for A, 34.91% for T, 19.47% for G, 14.25% for C, and its GC content was 33.72%. The mitogenome was composed of 13 protein-coding genes, 22 tranfer RNAs, and 2 ribosomal RNAs. Polygenetic analysis showed that the was more close to and . We speculated that the was evolved from freshwater species.
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http://dx.doi.org/10.1080/23802359.2019.1662744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707012PMC
September 2019

The complete mitochondrial genome of (Stimpson, 1860).

Mitochondrial DNA B Resour 2019 Sep 6;4(2):2834-2835. Epub 2019 Sep 6.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, Guangxi Autonomous Regions, China.

is a common and important shrimp species in the shallow waters of Indo-West Pacific. In this study, the mitochondrial genome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 15968 bp was 35.16% (5614 bp) for A, 33.51% (5351 bp) for T, 11.54% (1842 bp) for G, 19.80% (3161 bp) for C, and its GC content was 31.34%. The mitochondrial circular genome was composed of 13 protein-coding genes, 22 transfer RNAs, 1 D-loop and 2 ribosomal RNAs. Polygenetic analysis showed that the was more closed to .
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http://dx.doi.org/10.1080/23802359.2019.1660279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706880PMC
September 2019

Label-free and ultrasensitive electrochemical biosensor for the detection of EBV-related DNA based on [email protected]/AgNCs nanocomposites and lambda exonuclease-assisted target recycling.

Biosens Bioelectron 2019 Oct 19;143:111610. Epub 2019 Aug 19.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China. Electronic address:

A label-free and efficient electrochemical biosensor was developed for the ultrasensitive detection of EBV-related DNA by combing [email protected]/AgNCs nanocomposites with noncanonical lambda exonuclease (λ exo)-assisted target recycling (LNTR). The conjugates of AgDNCs, DNA/AgNCs and probe DNA ([email protected]/AgNCs conjugates) worked as not only ideal nanocarriers but also efficient electrochemical tags. LNTR didn't require phosphorylated substrates and could be triggered specifically by target DNA, leading to the recycling use of target DNA and the liberation of plentiful linker probes (LP). Subsequently, the LP hybridized with the capture probes on the electrode and then bond to [email protected]/AgNCs conjugates, generating a sensitive electric signal directly. What's more, the signal amplification effects of DNA/AgNCs and LNTR were investigated. Under the optimal conditions, the proposed method exhibited a wide linear range of 1 fM to 1 nM and the detection limit down to 0.38 fM. In addition, the developed biosensing method exhibited excellent specificity and was successfully applied to detect target DNA in complex biological matrix. The proposed biosensor without extra bio-labels may provide a promising platform in bioanalysis and biochemical research.
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http://dx.doi.org/10.1016/j.bios.2019.111610DOI Listing
October 2019

PtCo nanocubes/reduced graphene oxide hybrids and hybridization chain reaction-based dual amplified electrochemiluminescence immunosensing of antimyeloperoxidase.

Biosens Bioelectron 2019 Oct 30;142:111548. Epub 2019 Jul 30.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China. Electronic address:

Antimyeloperoxidase (anti-MPO) is regarded as one of the most important circulating autoantibodies for anti-neutrophil cytoplasm antibody (ANCA)-associated vasculitides (AAVs). Hence, it is crucial for highly sensitive detection of anti-MPO to monitor efficacy of AAVs in clinical diagnosis. Herein, a highly sensitive electrochemiluminescence (ECL) immunosensor for anti-MPO detection was constructed by combining reduced graphene oxide-supported PtCo nanocubes hybrids ([email protected]) with hybridization chain reaction (HCR) as signal amplification. Multiple ECL luminophores (Dox-ABEI) prepared by cross-linking of N-(aminobutyl)-N-(ethylisoluminol) (ABEI) and doxorubicin (Dox) were intercalated into dsDNA products of HCR, achieving the effective immobilization of ECL luminophores to obtain strong ECL emission. Benefiting from the efficient catalytic activity of [email protected] toward HO, the massive the superoxide radical (O-) were generated to further react with ABEI for ECL emission. Thus, the designed ECL immunoassay for anti-MPO detection exhibited excellent sensitivity of a concentration variation from 50 fg/mL to 1 ng/mL and a detection limit of 15.68 fg/mL. Importantly, this work proposed an enzyme-free ECL immunoassay with high sensitivity, excellent specificity for protein detection in clinical diagnosis.
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http://dx.doi.org/10.1016/j.bios.2019.111548DOI Listing
October 2019

The complete mitochondrial genome of (Philippi, 1848).

Mitochondrial DNA B Resour 2019 Jul 24;4(2):2742-2743. Epub 2019 Jul 24.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, Guangxi Autonomous Regions, China.

is a common and important component of mangrove ecosystem. In this study, the mitochondrial genome of was determined for the first time using next-generation sequencing; the overall base components of mitogenome consisting of 15633 bp was 31.14% for A, 35.70% for T, 16.65% for G, 16.51% for C, and its GC content was 33.16%. The mitochondrial circular genome was composed of 13 protein-coding genes, 22 tranfer RNAs, and 2 ribosomal RNAs. Polygenetic analysis showed that the was more closed to than and . We may speculate that the is evolved from freshwater species.
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http://dx.doi.org/10.1080/23802359.2019.1644549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706624PMC
July 2019

An innovative method for synergistic stabilization/solidification of Mn, NH-N, PO and F in electrolytic manganese residue and phosphogypsum.

J Hazard Mater 2019 08 11;376:212-222. Epub 2019 May 11.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China.

Electrolytic manganese residue (EMR) contains large quantities of manganese (Mn) and ammonia nitrogen (NH-N). Phosphogypsum (PG) contains plenty of phosphate (PO), fluorine (F) and some heavy metals. Separate storage of EMR and PG could seriously damage the ecological environment. In this study, synergistic stabilization/solidification (S/S) of EMR and PG was studied. The effects of EMR:PG mass ratio, S/S pH, solid-liquid ratio and temperature on the concentrations of NH-N, PO, Mn and F in the leaching solution, and the characteristics of EMR and PG were studied. Meanwhile, the synergistic S/S mechanisms of EMR and PG, and leaching test were investigated. The results showed that the concentrations of F, PO, NH-N and Mn in the leaching solution were 4.5 mg/L, 13.6 mg/L, 55.5 mg/L and 0.8 mg/L, respectively, when the mass ratio of EMR to PG was 1:2 and the pH was 9.0 adjusted by MgO after 20 days S/S. Manganese was mainly solidified as Mn(PO)·7HO and Mn(OH), and ammonia nitrogen was mainly stabilized as struvite; fluorine was mainly stabilized as (Mn, Ca, Mg)F, and phosphate was mainly solidified as (Mn, Ca, Mg)(PO) and (Mn, Ca, Mg)HPO. The leaching test results showed that PO and NH-N were reduced to 13.6 mg/L and 55.5 mg/L, respectively, and the concentrations of all the measured heavy metals and F were within the permitted level for the GB8978-1996 after 20 days S/S.
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http://dx.doi.org/10.1016/j.jhazmat.2019.05.017DOI Listing
August 2019

A amperometric immunosensor for sensitive detection of circulating tumor cells using a tyramide signal amplification-based signal enhancement system.

Biosens Bioelectron 2019 Apr 18;130:88-94. Epub 2019 Jan 18.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. Electronic address:

Herein, tyramide signal amplification (TSA)-based electrochemical immunosensor was exploited for highly sensitive detection of CTCs. In this immunosensor, the nucleolin-targeting aptamer AS1411 (CP) was used to specifically capture tumor cells, and a TSA-based signal enhancement system consisting of Pt [email protected]@CP composite as catalytic probe and tyramine functionalized infinite coordination polymer ([email protected]) as electroactive signal tag was applied to improve the detection sensitivity. Using HeLa cell as the model CTCs, after a sandwich reaction, CP-HeLa-Pt [email protected]@CP bioconjugates were formed on the electrode. Millions of [email protected] could be layer-by-layer deposited onto the target cell membrane by the catalysis of Pt [email protected]@CP. The developed immunorsensor could detect HeLa cell with a wide dynamic range from 2 to 2 × 10 cells/mL and a detection limit of 2 cells/mL. Most importantly, the amperometric immunosensor was successfully applied to discriminate tumor cells from white blood cells, exhibiting high specificity and sensitivity. In conclusion, this work demonstrates that the TSA-based signal enhancement system might be a potential alternative tool for the electrochemical measurement of trace amounts of CTCs in clinical diagnosis.
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http://dx.doi.org/10.1016/j.bios.2019.01.023DOI Listing
April 2019
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