Publications by authors named "Haipeng Yu"

69 Publications

N,N-Dimethylformamide solvent assisted hydrothermal pretreatment of Chlorella for coproduction of sugar, nitrogenous compounds and carbon dots.

Bioresour Technol 2021 Oct 19;344(Pt A):126143. Epub 2021 Oct 19.

Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, 483 Wush-an Road, Guangzhou 510642, China. Electronic address:

Microalgae are considered as a promising alternative to fossil fuels due to their ease of cultivation, short growth cycle and no occupation of cultivated land. In this study, N,N-Dimethylformamide (DMF) solvent was employed to assist hydrothermal pretreatment of Chlorella for coproduction of sugar, nitrogenous compounds and carbon dots (CDs). The effect of pretreatment conditions on the composition and pyrolysis bio-oil distribution of hydrothermal solid residues as well as CDs characteristic were investigated by varying the temperature (180-220 ℃) and reaction time (1-9 h). The results showed that pretreated residues had higher cellulose. And the yield of sugar and N-contained compounds reached 41.59% and 63.57% in the pyrolysis bio-oil of pretreated algae residues, respectively. Moreover, CDs obtained from hydrothermal solution fluoresced red under 365 nm excitation. The paper provides a new method for the complete utilization of microalgae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.126143DOI Listing
October 2021

Tunable and functional deep eutectic solvents for lignocellulose valorization.

Nat Commun 2021 09 14;12(1):5424. Epub 2021 Sep 14.

Stratingh Institute for Chemistry, University of Groningen, Groningen, The Netherlands.

Stabilization of reactive intermediates is an enabling concept in biomass fractionation and depolymerization. Deep eutectic solvents (DES) are intriguing green reaction media for biomass processing; however undesired lignin condensation is a typical drawback for most acid-based DES fractionation processes. Here we describe ternary DES systems composed of choline chloride and oxalic acid, additionally incorporating ethylene glycol (or other diols) that provide the desired 'stabilization' function for efficient lignocellulose fractionation, preserving the quality of all lignocellulose constituents. The obtained ethylene-glycol protected lignin displays high β-O-4 content (up to 53 per 100 aromatic units) and can be readily depolymerized to distinct monophenolic products. The cellulose residues, free from condensed lignin particles, deliver up to 95.9 ± 2.12% glucose yield upon enzymatic digestion. The DES can be recovered with high yield and purity and re-used with good efficiency. Notably, we have shown that the reactivity of the β-O-4 linkage in model compounds can be steered towards either cleavage or stabilization, depending on DES composition, demonstrating the advantage of the modular DES composition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-25117-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440657PMC
September 2021

Room temperature dissolving cellulose with a metal salt hydrate-based deep eutectic solvent.

Carbohydr Polym 2021 Nov 22;272:118473. Epub 2021 Jul 22.

Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China. Electronic address:

Abundant and renewable cellulose is a potential candidate for petroleum-derived synthetic polymers. However, the efficient dissolution of this material is problematic because of the high cost, severe reaction condition (e.g., high temperature) and environmentally unfriendly (e.g., toxic reagents, and solvent recyclability). Herein, to realize the room temperature dissolution of cellulose with an inexpensive and eco-friendly solvent, we design a novel low-cost deep eutectic solvent that is composed of zinc chloride, water and phosphoric acid for the efficient dissolution of cellulose. This solvent is featured as having both the superior hydrogen bonding acidity and the hydrogen bonding basicity, and thus can act as a hydrogen bond molecular scissors to cleave the hydrogen bonds within cellulose. In this process, microcrystalline cellulose can be easily dissolved in the solvent at room temperature with a dissolution ratio up to 15 wt%. The dissolved cellulose can also be recovered without any derivatization. The universality, recyclability and pilot production of dissolving cellulose using this solvent are also demonstrated. This work provides a new strategy for the design of novel deep eutectic solvent capable of disrupting the hydrogen bonds of cellulose under mild conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2021.118473DOI Listing
November 2021

Comparative analysis of bile culture and blood culture in patients with malignant biliary obstruction complicated with biliary infection.

J Cancer Res Ther 2021 Jul;17(3):726-732

Department of Intervention, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, Hebei Province, China.

Objective: This study is aimed to provide a clinical basis for the identification and treatment of patients with malignant biliary obstruction (MBO) complicated with biliary infection by comparing pathogenic bacteria detected in bile and blood cultures from these patients.

Materials And Methods: A total of 380 patients with MBO who received percutaneous transhepatic cholangic drainage from January 2004 to January 2019 were included in the study. A total of 90 patients were diagnosed with having MBO complicated with biliary infection, and bile and blood culture were simultaneously performed on these patients. The patients included 58 men and 32 women, ranging in age from 33 to 86 years old, with a mean age of 60.69 years.

Results: The detection rate using bile bacterial culture in patients with MBO complicated with biliary infection was significantly higher than that using blood culture, and there were significant differences in the two kinds of bacterial culture found positive bile and blood cultures from the same patients. Gram-positive cocci were dominant in the bile cultures and Gram-negative bacilli were dominant in the blood cultures. Therefore, it is necessary to conduct simultaneous bile bacterial culture and blood culture for patients with MBO complicated with biliary infection, especially those with severe or critical diseases.

Conclusions: It is vital to enable simultaneous bile bacterial culture and blood culture in patients with MBO complicated with biliary infection. Existing guidelines for the diagnosis and treatment of benign biliary infection are not applicable to patients with MBO complicated with biliary infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/jcrt.JCRT_1705_20DOI Listing
July 2021

Chinese expert consensus of image-guided irreversible electroporation for pancreatic cancer.

J Cancer Res Ther 2021 Jul;17(3):613-618

Department of Interventional Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Pancreatic cancer (PC) is a lethal disease with extremely high mortality. Although surgical resection is the optimal therapeutic approach for PC, about 30%-40% of those patients are not candidates for surgical resection when diagnosed. Chemotherapy and radiotherapy also could not claim a desirable effect on PC. The application of interventional radiology approaches is limited by unavoidable damage to the surrounding vessels or organs. By the superiority of mechanism and technology, IRE could ablate the tumor by creating irreversible pores on the membrane of PC cells with other tissues like vessels and pancreatic ducts untouched. This consensus gathers the theoretical basis and clinical experience from multiple Chinese medical centers, to provide the application principles and experience from Chinese experts in the IRE field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/jcrt.JCRT_1663_20DOI Listing
July 2021

Enhancing Hydrodeoxygenation of Bio-oil via Bimetallic Ni-V Catalysts Modified by Cross-Surface Migrated-Carbon from Biochar.

ACS Appl Mater Interfaces 2021 May 30;13(18):21482-21498. Epub 2021 Apr 30.

College of Materials and Energy, South China Agricultural University, Guangzhou 510640, China.

Aromatics from selective hydrodeoxygenation (HDO) of biomass-derived bio-oil are an ideal feedstock for replacing industrial fossil products. In this study, biochar-modified Hβ/Ni-V catalysts were prepared and tested in the atmospheric HDO of guaiacol and bio-oil to produce aromatics. Compared with unmodified Hβ/Ni-V, higher HDO activity was achieved in catalysts with all kinds of biochar modifications. Especially, the pine nut shell biochar (PB)-modified PB-Hβ-8/Ni-V showed the highest selectivity to aromatics (69.17%), mainly including benzene and toluene. Besides, under the conditions of 380 °C and weight hourly space velocity (WHSV) of 0.5 h, the cleavage of C-OH (C means the carbon in the benzene ring) was promoted to form more aromatics. Moreover, great recyclability (58.77% aromatics for the reactivated run-3 test) and efficient HDO of bio-oil (44.9% aromatic yield) were also achieved. Based on the characterization results, the enhanced aromatic selectivity of PB-Hβ-8/Ni-V was attributed to the synergetic effect between PB and Hβ/Ni-V. In detail, a stable surface migrated-carbon layer was formed on Hβ/Ni-V via the metal catalytic chemical vapor deposition (CVD) process of the pyrolysis PB volatiles. Simultaneously, a carbothermal reduction driven by the migrated-carbon took place to decorate the surface metals, obtaining more Ni and V active sites. With this synergism, increased Ni sites promoted H adsorption and dissociation, which improved the hydrogenation activity. Furthermore, the higher affinity of the reactant and increased oxygen vacancies both contributed to enhancing the selective surface adsorption of oxygenous groups and the cleavage of the C-OH bond, thus improving the deoxygenation activity. Therefore, the HDO activity was improved to form more target aromatics over biochar-modified catalysts. This work highlighted a potential avenue to develop economic and environmental catalysts for the upgrading of bio-oil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c05350DOI Listing
May 2021

Declining Oxygen Level as an Emerging Concern to Global Cities.

Environ Sci Technol 2021 06 27;55(12):7808-7817. Epub 2021 Apr 27.

Enlightening Bioscience Research Center, Mississauga L4X 2X7, Canada.

Rising CO concentration and temperatures in urban areas are now well-known, but the potential of an emerging oxygen crisis in the world's large cities has so far attracted little attention from the science community. Here, we investigated the oxygen balance and its related risks in 391 global large cities (with a population of more than 1 million people) using the oxygen index (O), which is the ratio of oxygen consumption to oxygen production. Our results show that the global urban areas, occupying only 3.8% of the global land surface, accounted for 39% (14.3 ± 1.5 Gt/yr) of the global terrestrial oxygen consumption during 2001-2015. We estimated that 75% of cities with a population more than 5 million had an O of greater than 100. Also, cities with larger O values were correlated with more frequent heatwaves and severe water withdrawals. In addition, cities with excessively large O values would likely experience severe hypoxia in extremely calm weather. Thus, mitigation measures should be adopted to reduce the urban O in order to build healthier and more sustainable cities.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.1c00553DOI Listing
June 2021

Identification of Quantitative Disease Resistance Loci Toward Four Species in Soybean.

Front Plant Sci 2021 30;12:644746. Epub 2021 Mar 30.

School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, United States.

In this study, four recombinant inbred line (RIL) soybean populations were screened for their response to infection by , , , and The parents, PI 424237A, PI 424237B, PI 408097, and PI 408029, had higher levels of resistance to these species in a preliminary screening and were crossed with "Williams," a susceptible cultivar. A modified seed rot assay was used to evaluate RIL populations for their response to specific species selected for a particular population based on preliminary screenings. Over 2500 single-nucleotide polymorphism (SNP) markers were used to construct chromosomal maps to identify regions associated with resistance to species. Several minor and large effect quantitative disease resistance loci (QDRL) were identified including one large effect QDRL on chromosome 8 in the population of PI 408097 × Williams. It was identified by two different disease reaction traits in , , and . Another large effect QDRL was identified on chromosome 6 in the population of PI 408029 × Williams, and conferred resistance to and . These large effect QDRL will contribute toward the development of improved soybean cultivars with higher levels of resistance to these common soil-borne pathogens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.644746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042330PMC
March 2021

MCM4 in human hepatocellular carcinoma: a potent prognostic factor associated with cell proliferation.

Biosci Trends 2021 May 12;15(2):100-106. Epub 2021 Mar 12.

Department of Interventional Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Hepatocellular carcinoma (HCC) remains a major public health problem. MCM4, a constitutive member of the minichromosomal maintenance protein family, has been reported to play a vital role in cancer malignancy behavior. However, the function of MCM4 in HCC remains largely unknown. The present study explored the specific role of MCM4 in HCC. The data from public datasets including TCGA and GTEx showed that MCM4 was overexpressed in HCC and significantly associated with poor prognosis. Immunohistochemistry results from 102 HCC patients suggested that high-level expression of MCM4 was correlated with tumor size. Then a series of in vivo and in vitro experiments were performed to investigate the function of MCM4 in HCC tumor cells. MCM4 silencing suppressed the cell proliferation and sphere formation of hepatoma cells. Moreover, silencing MCM4 significantly decreased the growth of tumors in a xenograft tumor model. In conclusion, the results of the present study indicated that MCM4 was a potential prognostic predictor associated with poor outcomes of HCC patients and even a therapeutic target for HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5582/bst.2021.01016DOI Listing
May 2021

Biopolymer Nanofibers for Nanogenerator Development.

Research (Wash D C) 2021 22;2021:1843061. Epub 2021 Feb 22.

Key Laboratory of Bio-Based Material Science and Technology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.

The development of nanogenerators (NGs) with optimal performances and functionalities requires more novel materials. Over the past decade, biopolymer nanofibers (BPNFs) have become critical sustainable building blocks in energy-related fields because they have distinctive nanostructures and properties and can be obtained from abundant and renewable resources. This review summarizes recent advances in the use of BPNFs for NG development. We will begin by introducing various strategies for fabricating BPNFs with diverse structures and performances. Then, we will systematically present the utilization of polysaccharide and protein nanofibers for NGs. We will mainly focus on the use of BPNFs to generate bulk materials with tailored structures and properties for assembling of triboelectric and piezoelectric NGs. The use of BPNFs to construct NGs for the generation of electricity from moisture and osmosis is also discussed. Finally, we illustrate our personal perspectives on several issues that require special attention with regard to future developments in this active field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.34133/2021/1843061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926511PMC
February 2021

Structural equation modeling for unraveling the multivariate genomic architecture of milk proteins in dairy cattle.

J Dairy Sci 2021 May 2;104(5):5705-5718. Epub 2021 Mar 2.

Department of Agronomy, Food Natural Resources, Animals and Environment, University of Padua, 35020 Legnaro (PD), Italy.

The aims of this study were to investigate potential functional relationships among milk protein fractions in dairy cattle and to carry out a structural equation model (SEM) GWAS to provide a decomposition of total SNP effects into direct effects and effects mediated by traits that are upstream in a phenotypic network. To achieve these aims, we first fitted a mixed Bayesian multitrait genomic model to infer the genomic correlations among 6 milk nitrogen fractions [4 caseins (CN), namely κ-, β-, α-, and α-CN, and 2 whey proteins, namely β-lactoglobulin (β-LG) and α-lactalbumin (α-LA)], in a population of 989 Italian Brown Swiss cows. Animals were genotyped with the Illumina BovineSNP50 Bead Chip v.2 (Illumina Inc.). A Bayesian network approach using the max-min hill-climbing (MMHC) algorithm was implemented to model the dependencies or independence among traits. Strong and negative genomic correlations were found between β-CN and α-CN (-0.706) and between β-CN and κ-CN (-0.735). The application of the MMHC algorithm revealed that κ-CN and β-CN seemed to directly or indirectly influence all other milk protein fractions. By integrating multitrait model GWAS and SEM-GWAS, we identified a total of 127 significant SNP for κ-CN, 89 SNP for β-CN, 30 SNP for α-CN, and 14 SNP for α-CN (mostly shared among CN and located on Bos taurus autosome 6) and 15 SNP for β-LG (mostly located on Bos taurus autosome 11), whereas no SNP passed the significance threshold for α-LA. For the significant SNP, we assessed and quantified the contribution of direct and indirect paths to total marker effect. Pathway analyses confirmed that common regulatory mechanisms (e.g., energy metabolism and hormonal and neural signals) are involved in the control of milk protein synthesis and metabolism. The information acquired might be leveraged for setting up optimal management and selection strategies aimed at improving milk quality and technological characteristics in dairy cattle.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3168/jds.2020-18321DOI Listing
May 2021

Forecasting dynamic body weight of nonrestrained pigs from images using an RGB-D sensor camera.

Transl Anim Sci 2021 Jan 17;5(1):txab006. Epub 2021 Jan 17.

Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

Average daily gain is an indicator of the growth rate, feed efficiency, and current health status of livestock species including pigs. Continuous monitoring of daily gain in pigs aids producers to optimize their growth performance while ensuring animal welfare and sustainability, such as reducing stress reactions and feed waste. Computer vision has been used to predict live body weight from video images without direct handling of the pig. In most studies, videos were taken while pigs were immobilized at a weighing station or feeding area to facilitate data collection. An alternative approach is to capture videos while pigs are allowed to move freely within their own housing environment, which can be easily applied to the production system as no special imaging station needs to be established. The objective of this study was to establish a computer vision system by collecting RGB-D videos to capture top-view red, green, and blue (RGB) and depth images of nonrestrained, growing pigs to predict their body weight over time. Over a period of 38 d, eight growers were video recorded for approximately 3 min/d, at the rate of six frames per second, and manually weighed using an electronic scale. An image-processing pipeline in Python using OpenCV was developed to process the images. Specifically, each pig within the RGB frame was segmented by a thresholding algorithm, and the contour of the pig was identified to extract its length and width. The height of a pig was estimated from the depth images captured by the infrared depth sensor. Quality control included removing pigs that were touching the fence and sitting, as well as those showing extremely distorted shape or motion blur owing to their frequent movement. Fitting all of the morphological image descriptors simultaneously in linear mixed models yielded prediction coefficients of determination of 0.72-0.98, 0.65-0.95, 0.51-0.94, and 0.49-0.93 for 1-, 2-, 3-, and 4-d ahead forecasting, respectively, of body weight in time series cross-validation. Based on the results, we conclude that our RGB-D sensor-based imaging system coupled with the Python image-processing pipeline could potentially provide an effective approach to predict the live body weight of nonrestrained pigs from images.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/tas/txab006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906448PMC
January 2021

ABCB1 and ABCG2 restricts the efficacy of gedatolisib (PF-05212384), a PI3K inhibitor in colorectal cancer cells.

Cancer Cell Int 2021 Feb 16;21(1):108. Epub 2021 Feb 16.

Department of Interventional Treatment, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin, 300060, China.

Background: Overexpression of ABC transporters is a big challenge on cancer therapy which will lead cancer cells resistance to a series of anticancer drugs. Gedatolisib is a dual PI3K and mTOR inhibitor which is under clinical evaluation for multiple types of malignancies, including colorectal cancer. The growth inhibitory effects of gedatolisib on colorectal cancer cells have been specifically studied. However, the role of ABC transporters on gedatolisib resistance remained unclear. In present study, we illustrated the role of ABC transporters on gedatolisib resistance in colorectal cancer cells.

Methods: Cell viability investigations of gedatolisib on colorectal cancer cells were determined by MTT assays. The verapamil and Ko143 reversal studies were determined by MTT assays as well. ABCB1 and/or ABCG2 siRNA interference assays were conducted to verify the role of ABCB1- and ABCG2-overexpression on gedatolisib resistance. The accumulation assays of gedatolisib were conducted using tritium-labeled paclitaxel and mitoxantrone. The effects of gedatolisib on ATPase activity of ABCB1 or ABCG2 were conducted using PREDEASY ATPase Kits. The expression level of ABCB1 and ABCG2 after gedatolisib treatment were conducted by Western blotting and immunofluorescence assays. The well-docked position of gedatolisib with crystal structure of ABCB1 and ABCG2 were simulated by Autodock vina software. One-way ANOVA was used for the statistics analysis.

Results: Gedatolisib competitively increased the accumulation of tritium-labeled substrate-drugs in both ABCB1- and ABCG2-overexpression colorectal cancer cells. Moreover, gedatolisib significantly increased the protein expression level of ABCB1 and ABCG2 in colorectal cancer cells. In addition, gedatolisib remarkably simulated the ATPase activity of both ABCB1 and ABCG2, suggesting that gedatolisib is a substrate drug of both ABCB1 and ABCG2 transporters. Furthermore, a gedatolisib-resistance colorectal cancer cell line, SW620/GEDA, was selected by increasingly treatment with gedatolisib to SW620 cells. The SW620/GEDA cell line was proved to resistant to gedatolisib and a series of chemotherapeutic drugs, except cisplatin. The ABCB1 and ABCG2 were observed overexpression in SW620/GEDA cell line.

Conclusions: These findings suggest that overexpression of ABCB1 and ABCG2 may restrict the efficacy of gedatolisib in colorectal cancer cells, while co-administration with ABC transporter inhibitors may improve the potency of gedatolisib.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-021-01800-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885361PMC
February 2021

GCA: an R package for genetic connectedness analysis using pedigree and genomic data.

BMC Genomics 2021 Feb 15;22(1):119. Epub 2021 Feb 15.

Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, 24061, VA, USA.

Background: Genetic connectedness is a critical component of genetic evaluation as it assesses the comparability of predicted genetic values across units. Genetic connectedness also plays an essential role in quantifying the linkage between reference and validation sets in whole-genome prediction. Despite its importance, there is no user-friendly software tool available to calculate connectedness statistics.

Results: We developed the GCA R package to perform genetic connectedness analysis for pedigree and genomic data. The software implements a large collection of various connectedness statistics as a function of prediction error variance or variance of unit effect estimates. The GCA R package is available at GitHub and the source code is provided as open source.

Conclusions: The GCA R package allows users to easily assess the connectedness of their data. It is also useful to determine the potential risk of comparing predicted genetic values of individuals across units or measure the connectedness level between training and testing sets in genomic prediction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-021-07414-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885574PMC
February 2021

Modeling multiple phenotypes in wheat using data-driven genomic exploratory factor analysis and Bayesian network learning.

Plant Direct 2021 Jan 25;5(1):e00304. Epub 2021 Jan 25.

Department of Animal and Poultry Sciences Virginia Polytechnic Institute and State University Blacksburg VA USA.

Inferring trait networks from a large volume of genetically correlated diverse phenotypes such as yield, architecture, and disease resistance can provide information on the manner in which complex phenotypes are interrelated. However, studies on statistical methods tailored to multidimensional phenotypes are limited, whereas numerous methods are available for evaluating the massive number of genetic markers. Factor analysis operates at the level of latent variables predicted to generate observed responses. The objectives of this study were to illustrate the manner in which data-driven exploratory factor analysis can map observed phenotypes into a smaller number of latent variables and infer a genomic latent factor network using 45 agro-morphological, disease, and grain mineral phenotypes measured in synthetic hexaploid wheat lines (.). In total, eight latent factors including grain yield, architecture, flag leaf-related traits, grain minerals, yellow rust, two types of stem rust, and leaf rust were identified as common sources of the observed phenotypes. The genetic component of the factor scores for each latent variable was fed into a Bayesian network to obtain a trait structure reflecting the genetic interdependency among traits. Three directed paths were consistently identified by two Bayesian network algorithms. Flag leaf-related traits influenced leaf rust, and yellow rust and stem rust influenced grain yield. Additional paths that were identified included flag leaf-related traits to minerals and minerals to architecture. This study shows that data-driven exploratory factor analysis can reveal smaller dimensional common latent phenotypes that are likely to give rise to numerous observed field phenotypes without relying on prior biological knowledge. The inferred genomic latent factor structure from the Bayesian network provides insights for plant breeding to simultaneously improve multiple traits, as an intervention on one trait will affect the values of focal phenotypes in an interrelated complex trait system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/pld3.304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833463PMC
January 2021

Gold nanoplates with superb photothermal efficiency and peroxidase-like activity for rapid and synergistic antibacterial therapy.

Chem Commun (Camb) 2021 Feb;57(9):1133-1136

Institute for Chemical Biology & Biosensing, and College of Life Sciences, and School of Pharmacy, Medical College, Qingdao University, 308 Ningxia Road, Qingdao 266071, China.

Gold nanoplates (AuNPTs) exhibit outstanding photothermal conversion efficiency (68.5%) and peroxidase-like activity. The combination of the very low H2O2 concentration (0.1 mM) and the low AuNPT dosage (50 μg mL-1) with 808 nm laser irradiation (1 W cm-2, 3 min) shows excellent synergistic antibacterial ability and healing of MRSA-infected wounds in vivo.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cc06925fDOI Listing
February 2021

Should the Oddis sphincter be retained? A clinical analysis of biliary metal stent implantation in patients with malignant obstructive jaundice.

J Cancer Res Ther 2020 Sep;16(5):1119-1124

Department of Interventional Treatment, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Objective: We sought to analyze the efficacy and safety of preserving the Oddis sphincter during metallic biliary stent implantation in patients with malignant obstructive jaundice.

Materials And Methods: In a retrospective analysis, 133 patients with malignant obstructive jaundice who were admitted to our hospital from January 2010 to January 2017 and who underwent metallic biliary stent implantation were divided into two groups - the Oddis sphincter retention group (n = 55) and the Oddis sphincter nonretention group (n = 78) - according to whether the Oddis sphincter was left untouched during stent placement. The patient clinical data as well as information on complications, time of stent patency, improvement in liver function, and decline of serum bilirubin were reviewed and evaluated. Statistical analysis was performed using the Statistical Package for the Social Sciences version 19.0 (IBM Corp., Armonk, NY, USA, USA) and Prism version 7 (GraphPad Software, San Diego, CA, USA).

Results: The median follow-up time was 9.6 months (range: 1-20 months) and there was no significant difference in general clinical information between the two groups. However, the incidence rates of acute biliary infection, recurrent biliary infection, acute pancreatitis, chronic pancreatitis, and asymptomatic pancreatic enzyme levels were higher in the Oddis sphincter retention group and the differences were all statistically significant (P < 0.05). Conversely, there were no significant differences in bilirubin decline, liver function improvement, and stent patency between the two groups (P > 0.05).

Conclusion: Leaving the Oddis sphincter untouched during biliary stent placement can reduce the incidence of postoperative complications, while there was no effect on stent patency or jaundice relief. Therefore, it is recommended to preserve the Oddis sphincter when the stenosis is more than 3 cm above the duodenal papilla.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/jcrt.JCRT_220_20DOI Listing
September 2020

An assessment of genomic connectedness measures in Nellore cattle.

J Anim Sci 2020 Nov;98(11)

Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA.

An important criterion to consider in genetic evaluations is the extent of genetic connectedness across management units (MU), especially if they differ in their genetic mean. Reliable comparisons of genetic values across MU depend on the degree of connectedness: the higher the connectedness, the more reliable the comparison. Traditionally, genetic connectedness was calculated through pedigree-based methods; however, in the era of genomic selection, this can be better estimated utilizing new approaches based on genomics. Most procedures consider only additive genetic effects, which may not accurately reflect the underlying gene action of the evaluated trait, and little is known about the impact of non-additive gene action on connectedness measures. The objective of this study was to investigate the extent of genomic connectedness measures, for the first time, in Brazilian field data by applying additive and non-additive relationship matrices using a fatty acid profile data set from seven farms located in the three regions of Brazil, which are part of the three breeding programs. Myristic acid (C14:0) was used due to its importance for human health and reported presence of non-additive gene action. The pedigree included 427,740 animals and 925 of them were genotyped using the Bovine high-density genotyping chip. Six relationship matrices were constructed, parametrically and non-parametrically capturing additive and non-additive genetic effects from both pedigree and genomic data. We assessed genome-based connectedness across MU using the prediction error variance of difference (PEVD) and the coefficient of determination (CD). PEVD values ranged from 0.540 to 1.707, and CD from 0.146 to 0.456. Genomic information consistently enhanced the measures of connectedness compared to the numerator relationship matrix by at least 63%. Combining additive and non-additive genomic kernel relationship matrices or a non-parametric relationship matrix increased the capture of connectedness. Overall, the Gaussian kernel yielded the largest measure of connectedness. Our findings showed that connectedness metrics can be extended to incorporate genomic information and non-additive genetic variation using field data. We propose that different genomic relationship matrices can be designed to capture additive and non-additive genetic effects, increase the measures of connectedness, and to more accurately estimate the true state of connectedness in herds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jas/skaa289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792904PMC
November 2020

Designing Hybrid Chiral Photonic Films with Circularly Polarized Room-Temperature Phosphorescence.

ACS Nano 2020 Sep 26;14(9):11130-11139. Epub 2020 Aug 26.

Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin 150040, China.

Circular polarized luminescence (CPL) is essential to chiral sciences and photonic technologies, but the achievement of circular polarized room-temperature phosphorescence (CPRTP) remains a great challenge due to the instability of triplet state excitons. Herein, we found that dual CPL and CPRTP were demonstrated by hybrid chiral photonic films designed by the coassembly of cellulose nanocrystals (CNCs), poly(vinyl alcohol) (PVA), and carbon dots (CDs). Tunable photonic band gaps were achieved by regulating the ratio of CNC/PVA in the hybrid films, leading to tunable CPL with invertible handedness, tunable wavelengths, and considerable dissymmetric factors () up to -0.27. In particularly, triplet excitons produced by CDs were stable in the chiral photonic crystal environment, resulting in tunable right-handed CPRTP with long lifetimes up to 103 ms and large RTP dissymmetric factors () up to -0.47. Moreover, patterned films with multiple polarized features were demonstrated by a mold technique.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.0c02060DOI Listing
September 2020

Deciphering Cattle Temperament Measures Derived From a Four-Platform Standing Scale Using Genetic Factor Analytic Modeling.

Front Genet 2020 12;11:599. Epub 2020 Jun 12.

Department of Animal Sciences, North Dakota State University, Fargo, ND, United States.

The animal's reaction to human handling (i.e., temperament) is critical for work safety, productivity, and welfare. Subjective phenotyping methods have been traditionally used in beef cattle production. Even so, subjective scales rely on the evaluator's knowledge and interpretation of temperament, which may require substantial experience. Selection based on such subjective scores may not precisely change temperament preferences in cattle. The objectives of this study were to investigate the underlying genetic interrelationships among temperament measurements using genetic factor analytic modeling and validate a movement-based objective method (four-platform standing scale, FPSS) as a measure of temperament. Relationships among subjective methods of docility score (DS), temperament score (TS), 12 qualitative behavior assessment (QBA) attributes and objective FPSS including the standard deviation of total weight on FPSS over time (SSD) and coefficient of variation of SSD (CVSSD) were investigated using 1,528 calves at weaning age. An exploratory factor analysis (EFA) identified two latent variables account for TS and 12 QBA attributes, termed and from their characteristics. Inclusion of DS in EFA was not a good fit because it was evaluated under restraint and other measures were not. A Bayesian confirmatory factor analysis inferred the and scores discovered in EFA. This was followed by fitting a pedigree-based Bayesian multi-trait model to characterize the genetic interrelationships among , DS, SSD, and CVSSD. Estimates of heritability ranged from 0.18 to 0.4 with the posterior standard deviation averaging 0.06. The factors of and exhibited a large negative genetic correlation of -0.92. Moderate genetic correlation was found between DS and (0.36), (-0.31), SSD (0.42), and CVSSD (0.34) as well as FPSS with (CVSSD: 0.35; SSD: 0.42) and (CVSSD: -0.35; SSD: -0.4). Correlation coefficients indicate selection could be performed with either and have similar outcomes. We contend that genetic factor analytic modeling provided a new approach to unravel the complexity of animal behaviors and FPSS-like measures could increase the efficiency of genetic selection by providing automatic, objective, and consistent phenotyping measures that could be an alternative of DS, which has been widely used in beef production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2020.00599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304504PMC
June 2020

Stimuli-responsive composite biopolymer actuators with selective spatial deformation behavior.

Proc Natl Acad Sci U S A 2020 06 10;117(25):14602-14608. Epub 2020 Jun 10.

Department of Biomedical Engineering, Tufts University, Medford, MA 02155;

Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors. These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility. Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods. Programmed site-selective actuation can be predicted and folded into three-dimensional (3D) origami-like shapes. The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements. Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2002996117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322001PMC
June 2020

Wood-Derived Carbon Materials and Light-Emitting Materials.

Adv Mater 2021 Jul 2;33(28):e2000596. Epub 2020 Jun 2.

Key laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Hexing Road 26, Harbin, 150040, P. R. China.

Wood is a sustainable and renewable material that naturally has a hierarchical structure. Cellulose, hemicellulose, and lignin are the three main components of wood. The unique physical and chemical properties of wood and its derivatives endow them with great potential as resources to fabricate advanced materials for use in bioengineering, flexible electronics, and clean energy. Nevertheless, comprehensive information on wood-derived carbon and light-emitting materials is scarce, although much excellent progress has been made in this area. Here, the unique characteristics of wood-derived carbon and light-emitting materials are summarized, with regard to the fabrication principles, properties, applications, challenges, and future prospects of wood-derived carbon and light-emitting materials, with the aim of deepening the understanding and inspiring new ideas in the area of advanced wood-based materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202000596DOI Listing
July 2021

Fate and behavior of Sanchi oil spill transported by the Kuroshio during January-February 2018.

Mar Pollut Bull 2020 Mar 22;152:110917. Epub 2020 Jan 22.

National Satellite Ocean Application Service (NSOAS), MNR, Beijing 100081, China.

The fate and behavior of the Sanchi oil spill during January-February 2018 was simulated by coupling an oil spill model and satellite observations with meteo-oceanographic forcing. Extensive validation tests were performed for winds, currents, surface slick, stranded oil and oil fate. A series of hindcast experiments was designed to take into account the uncertainties in oil amount, environmental forcing and model parameters. The simulations confirmed that the stable large-scale Kuroshio acted as the primary driving force. Most oil followed the Kuroshio's large-meander path, rapidly passing through the East China Sea to the waters south of Japan. The wind, appearing as the secondary transport factor, did not change the path of this large-scale current, but did contribute to the drift of surface oil. The different fates for heavy fuel oil and condensate in the accident were also compared quantitatively and discussed in this study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.marpolbul.2020.110917DOI Listing
March 2020

Matrine inhibits proliferation and migration of HepG2 cells by downregulating ERK1/2 signaling pathways.

J Cancer Res Ther 2020 ;16(2):209-214

Department of Gastroenterology, The 940 Hospital of Joint Logistic Support Force of People's Liberation Army, Lanzhou, China.

Objective: To research the effect of matrine on the proliferation and migration of HepG2 cells through extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway.

Methods: HepG2 cell was selected and divided into blank control group, experimental group (matrine 1, 2, and 4 mg/mL), and positive control group (PD98059, ERK1/2 inhibitor). MTT measure was used to detect the effective time and concentration which matrine inhibits HepG2 cells. After 24 h, the effect of effective concentration of matrine on the of morphological changing HepG2 cells was observed. The invasion ability was assayed by transwell method, the expression of ERK1/2 and pERK1/2 were detected through Western blot, and reverse transcription polymerase chain reaction was used to test the expression level of ERK1/2 mRNA.

Results: With the increase of matrine concentration, the number of adherent HepG2 cells gradually decreased, the morphologic changes gradually became spherical, some cell morphology was incomplete, and even cell fragments appeared. The proliferation and invasion ability of HepG2 cells decreased. The expression of ERK1/2, pERK1/2, and ERK1/2 mRNA downregulated with the increase of matrine concentration (P < 0.05).

Conclusion: Matrine inhibits the proliferation and migration of HepG2 cells by downregulating the ERK1/2 signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/jcrt.JCRT_331_19DOI Listing
September 2020

Cellulose-Based Flexible Functional Materials for Emerging Intelligent Electronics.

Adv Mater 2021 Jul 20;33(28):e2000619. Epub 2020 Apr 20.

Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin, 150040, P. R. China.

There is currently enormous and growing demand for flexible electronics for personalized mobile equipment, human-machine interface units, wearable medical-healthcare systems, and bionic intelligent robots. Cellulose is a well-known natural biopolymer that has multiple advantages including low cost, renewability, easy processability, and biodegradability, as well as appealing mechanical performance, dielectricity, piezoelectricity, and convertibility. Because of its multiple merits, cellulose is frequently used as a substrate, binder, dielectric layer, gel electrolyte, and derived carbon material for flexible electronic devices. Leveraging the advantages of cellulose to design advanced functional materials will have a significant impact on portable intelligent electronics. Herein, the unique molecular structure and nanostructures (nanocrystals, nanofibers, nanosheets, etc.) of cellulose are briefly introduced, the structure-property-application relationships of cellulosic materials summarized, and the processing technologies for fabricating cellulose-based flexible electronics considered. The focus then turns to the recent advances of cellulose-based functional materials toward emerging intelligent electronic devices including flexible sensors, optoelectronic devices, field-effect transistors, nanogenerators, electrochemical energy storage devices, biomimetic electronic skins, and biological detection devices. Finally, an outlook of the potential challenges and future prospects for developing cellulose-based wearable devices and bioelectronic systems is presented.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202000619DOI Listing
July 2021

Accelerated dryland expansion regulates future variability in dryland gross primary production.

Nat Commun 2020 04 3;11(1):1665. Epub 2020 Apr 3.

Atmospheric Sciences and Global Change Division, Pacific Northwest National Laboratory, Richland, WA, USA.

Drylands cover 41% of Earth's surface and are the largest source of interannual variability in the global carbon sink. Drylands are projected to experience accelerated expansion over the next century, but the implications of this expansion on variability in gross primary production (GPP) remain elusive. Here we show that by 2100 total dryland GPP will increase by 12 ± 3% relative to the 2000-2014 baseline. Because drylands will largely expand into formerly productive ecosystems, this increase in dryland GPP may not increase global GPP. Further, GPP per unit dryland area will decrease as degradation of historical drylands outpaces the higher GPP of expanded drylands. Dryland expansion and climate-induced conversions among sub-humid, semi-arid, arid, and hyper-arid subtypes will lead to substantial changes in regional and subtype contributions to global dryland GPP variability. Our results highlight the vulnerability of dryland subtypes to more frequent and severe climate extremes and suggest that regional variations will require different mitigation strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-15515-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125214PMC
April 2020

Detection of circulating tumor DNA from non-small cell lung cancer brain metastasis in cerebrospinal fluid samples.

Thorac Cancer 2020 03 13;11(3):588-593. Epub 2020 Jan 13.

Department of Interventional Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Background: Evaluating the molecular characteristics of brain metastases is limited by difficult access and by the blood-brain barrier, which prevents circulating tumor DNA (ctDNA) from entering the blood. In this study, we aimed to compare the sequencing results from cerebrospinal fluid (CSF) ctDNA versus plasma ctDNA, plasma circulating tumor cells (CTCs), and brain tissue specimens from patients with brain metastasis from non-small cell lung cancer (NSCLC).

Methods: This was a prospective study of 21 consecutive patients with NSCLC and brain metastasis diagnosed between April 2018 and January 2019. Samples of CSF and peripheral blood were obtained from all 21 patients. Brain tissues were obtained from five patients after surgical resection. Next-generation sequencing was performed using the Ion system. Single nucleotide variants (SNVs) and small insertions or deletions (indels) were searched.

Results: Mutations were detected in the CSF ctDNA of 20 (95.2%) patients. The detection rate of epidermal growth factor receptor (EGFR) mutations in CSF ctDNA was 57.1% (12/21) whereas this rate was only 23.8% (5/21) in peripheral blood ctDNA and in CTCs. EGFR mutations were found in the CSF of 9 of 11 (81.8%) patients with leptomeningeal metastases, as compared with three of 10 (30%) patients with brain parenchymal metastases. Mutations were also detected in KIT, PIK3CA, TP53, SMAD4, ATM, SMARCB1, PTEN, FLT3, GNAS, STK11, MET, CTNNB1, APC, FBXW7, ERBB4, and KDR (all >10%). The status of EGFR and TP53 mutations was consistent between CSF ctDNA and brain lesion tissue in all five patients.

Conclusion: Sequencing of CSF ctDNA revealed specific mutation patterns in driver genes among patients with NSCLC and brain metastasis.

Key Points: In some small-sample studies, the importance of cerebrospinal fluid in guiding the treatment of cancerous brain lesions has been verified in that it may reflect genomic mutations of brain tumors relatively accurately. Cerebrospinal fluid is a new form of liquid biopsy that can be helpful in improving the management of patients with brain metastasis from non-small cell lung cancer by detecting genetic abnormalities specific to brain metastases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1759-7714.13300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049513PMC
March 2020

Production of Nanocellulose Using Hydrated Deep Eutectic Solvent Combined with Ultrasonic Treatment.

ACS Omega 2019 May 15;4(5):8539-8547. Epub 2019 May 15.

Key Laboratory of Bio-based Material Science and Technology of Ministry of Education, Northeast Forestry University, Harbin 150040, P. R. China.

Pretreatment approaches are highly desirable to improve the commercial viability of nanocellulose production. In this study, we propose a new approach to mass produce nanocellulose using a hydrated choline chloride/oxalic acid dihydrate deep eutectic solvent (DES) combined with an ultrasonic process. The hydrogen bond acidity, polarizability, and solvation effect reflected by the Kamlet-Taft solvatochromic parameters did not decrease even after the addition of large amounts of water. Instead, the water facilitated the ionization of H and delocalization of Cl ions, forming new Cl-HO ionic hydrogen and oxalate-HO hydrogen bonds, which are critical for improving the solvent characteristics. One pass of kraft pulp through the hydrated DESs (80 °C, 1 h) was sufficient to dissociate the kraft pulp into cellulose nanofibers or cellulose nanocrystals using an 800 W ultrasonic treatment. The present study represents an alternative route for the kraft pulp pretreatment and the large-scale production of nanocellulose.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.9b00519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6648160PMC
May 2019

Deep Eutectic Solvent-Assisted In Situ Wood Delignification: A Promising Strategy To Enhance the Efficiency of Wood-Based Solar Steam Generation Devices.

ACS Appl Mater Interfaces 2019 Jul 9;11(29):26032-26037. Epub 2019 Jul 9.

Key Laboratory of Bio-based Material Science and Technology of Ministry of Education , Northeast Forestry University , Hexing Road 26 , Harbin 150040 , P.R. China.

Wood-based solar steam generation devices (W-SSGDs) show great promise for desalination and wastewater treatment because they are inexpensive and sustainable. Technical methods for enhancing the efficiency of W-SSGDs have, so far, mainly focused on the optimization of solar-to-thermal conversion efficiency, whereas the properties of the wood matrix have been ignored. Here, we have designed a strategy using deep eutectic solvents to remove lignin from bulk wood in situ for the fabrication of a high-performance W-SSGD (DW-SSGD). Wood delignification increases the water transportation capacity while reducing thermal conductivity and conductive heat loss in the wood matrix. The improved properties of delignified wood allowed us to construct a high-performance DW-SSGD with a steam generation efficiency of 89% and an evaporation rate as high as 1.3 kg m h at 1 sun (100 mW cm). To the best of our knowledge, the performance reported here is the highest for a W-SSGD under one solar irradiation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.9b08244DOI Listing
July 2019
-->