Publications by authors named "Haili Zheng"

3 Publications

  • Page 1 of 1

Preparation and Biological Property Evaluation of Novel Cationic Lipid-Based Liposomes for Efficient Gene Delivery.

AAPS PharmSciTech 2021 Jan 3;22(1):22. Epub 2021 Jan 3.

Pharmacy Department, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.

Novel cationic lipid-based liposomes prepared using an amphiphilic cationic lipid material, N,N-dimethyl-(N',N'-di-stearoyl-1-ethyl)1,3-diaminopropane (DMSP), have been proposed to enhance the transfection of nucleic acids. Herein, we designed and investigated liposomes prepared using DMSP, soybean phosphatidylcholine, and cholesterol. This novel gene vector has high gene loading capabilities and excellent protection against nuclease degradation. An in vitro study showed that the liposomes had lower toxicity and superior cellular uptake and transfection efficiency compared with Lipofectamine 2000. An endosomal escape study revealed that the liposomes demonstrated high endosomal escape and released their genetic payload in the cytoplasm efficiently. Mechanistic studies indicated that the liposome/nucleic acid complexes entered cells through energy-dependent endocytosis that was mediated by fossa proteins. These results suggest that such cationic lipid-based liposome vectors have potential for clinical gene delivery.
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http://dx.doi.org/10.1208/s12249-020-01868-wDOI Listing
January 2021

Fabrication of an Inverse Size-Selective Membrane Using an Electrospun Nanofiber Mat as a Template.

ACS Appl Mater Interfaces 2020 Apr 17;12(13):15686-15694. Epub 2020 Mar 17.

Department of Chemistry, Queen's University, 90 Bader Lane, Kingston, Ontario, Canada K7L 3N6.

Size-selective membranes have applications in water purification and other areas. Although many methods have been developed for the fabrication of nanoporous membranes, each method has its limitations. For example, thin films containing uniform nanochannels can be prepared from block copolymer precursors. However, block copolymers are expensive. The electrospinning of a polymer solution can yield long nanofibers that fold into mats and the diameters of these fibers can be tuned from nanometers to micrometers. Infusing another polymer into the voids between these fibers and subsequently removing the nanofiber template should yield an inverse porous membrane, complementary in pore structure to the original nanofiber mat membrane. In this paper, we report on the fabrication of these membranes. We discovered that the flux across such membranes increased by thermally annealing the nanofiber mats under pressure before the infusion of the second polymer and by etching the surfaces of the final membrane with plasma to expose the encapsulated nanochannels. We further discovered that the size of the pores formed at the junction of the fused nanofibers and, eventually, the nanotubes governed the size selectivity of the final membrane. The pore size at the junctions increased by increasing the thermal annealing temperature and, thus, the extent of fiber fusion. The developed methodology is general and should be useful for the fabrication of nanoporous membranes from different materials possessing pore diameters that are governed by the diameter of the templating nanofibers and the extent of their fusion.
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http://dx.doi.org/10.1021/acsami.0c00065DOI Listing
April 2020

Clematichinenoside inhibits VCAM-1 and ICAM-1 expression in TNF-α-treated endothelial cells via NADPH oxidase-dependent IκB kinase/NF-κB pathway.

Free Radic Biol Med 2015 Jan 13;78:190-201. Epub 2014 Nov 13.

Department of Physiology, China Pharmaceutical University, Nanjing, Jiangsu, China. Electronic address:

Proinflammatory cytokine TNF-α-induced adhesion of leukocytes to endothelial cells plays a critical role in the early stage of atherosclerosis. Oxidative stress and redox-sensitive transcription factors are implicated in the process. Thus, compounds that mediate intracellular redox status and regulate transcription factors are of great therapeutic interest. Clematichinenoside (AR), a triterpene saponin isolated from the root of Clematis chinensis Osbeck, was previously demonstrated to have anti-inflammatory and antioxidative properties. However, little is known about the exact mechanism underlying these actions. Thus we performed a detailed study on its effect on leukocytes-endothelial cells adhesion with TNF-α-stimulated human umbilical vein endothelial cells (HUVECs) and cell-free systems. First, we found that AR reduced TNF-α-induced VCAM-1 and ICAM-1 expression and their promoter activity, inhibited translocation of p65 and phosphorylation of IκBα, suppressed IκB kinase-β (IKK-β) activity, lowered O2(∙-) and H2O2 levels, tackled p47(phox) translocation, and decreased NOX4 NADPH oxidase expression. Second, we showed that AR exhibited no direct free radical scavenging ability in cell-free systems at concentrations that were used in intact cells. Besides, AR had no direct effect on the activity of IKK-β that was extracted from TNF-α-stimulated HUVECs. We also found that p47 translocation, NOX4 expression, and reactive oxygen species (ROS) levels were up-regulated before IκB phosphorylation in TNF-α-induced HUVECs. Moreover, TNF-α-enhanced IKK-β activity was also inhibited by (polyethylene glycol) PEG-catalase, N-acetylcysteine (NAC), and vitamin E. In conclusion, these results suggest that AR reduces VCAM-1 and ICAM-1 expression through NADPH oxidase-dependent IKK/NF-κB pathways in TNF-α-induced HUVECs, which finally suppress monocyte-HUVECs adhesion. This compound is potentially beneficial for early-stage atherosclerosis.
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http://dx.doi.org/10.1016/j.freeradbiomed.2014.11.004DOI Listing
January 2015
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