Publications by authors named "Haijiao Wang"

79 Publications

Myeloid-Derived Suppressor Cells in Patients With Acute Pancreatitis With Increased Inhibitory Function.

Front Immunol 2022 14;13:840620. Epub 2022 Jul 14.

Department of Hepatology, The First Hospital of Jilin University, Changchun, China.

Acute pancreatitis (AP) is pancreatic or systemic inflammation without or with motion organ dysfunction. Severe acute pancreatitis (SAP) is the main cause of death for patients with AP. A pro-/anti-inflammatory imbalance is considered the key regulation of disease severity. However, the real mechanism of SAP remains unclear. This study aimed to identify the frequency and specific roll of myeloid-derived suppressor cell (MDSC) in AP. We evaluated MDSC frequency and disease severity by analyzing MDSCs in the peripheral blood of healthy controls (HCs) and patients with mild acute pancreatitis (MAP) and SAP by flow cytometry. We also compared the frequency and inhibitory ability of MDSCs from HCs and SAP, and finally detected the reason for the difference in inhibitory ability. AP was marked by expansion of MDSCs as well as its subsets, granulocytic MDSCs (G-MDSCs) and monocytic MDSCs (M-MDSCs). The proportion of MDSC in the peripheral blood mononuclear cells of patients with AP was increased and positively correlated with AP severity. The frequency of MDSC was decreased after treatment compared with pre-treatment. CD3+ T cells were remarkably inhibited by MDSC derived from the patients with SAP. In the expression of arginase-1 (Arg-1) and reactive oxygen species (ROS), the MDSCs from patients with SAP increased. These findings demonstrated that MDSCs expanded in the peripheral blood in patients with AP, especially in those with SAP. Moreover, the inhibitory ability of MDSCs was increased in the patients with SAP compared with that in the HCs. The enhanced suppressive function was possibly caused by an overexpression of Arg-1 and ROS.
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http://dx.doi.org/10.3389/fimmu.2022.840620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329796PMC
July 2022

Retraction notice to "Long non-coding RNA LINC00460 promotes epithelial ovarian cancer progression by regulating microRNA-338-3p" [Biomed. Pharmacother. 108 (2018) 1022-1028].

Biomed Pharmacother 2022 07 11:113354. Epub 2022 Jul 11.

Department of Gynecology, The First Hospital of Jilin University, Changchun 130021, China.

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http://dx.doi.org/10.1016/j.biopha.2022.113354DOI Listing
July 2022

Flexible CuS-embedded human serum albumin hollow nanocapsules with peroxidase-like activity for synergistic sonodynamic and photothermal cancer therapy.

Nanoscale 2022 Jul 14;14(27):9702-9714. Epub 2022 Jul 14.

Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210046, Jiangsu, P. R. China.

Nanoparticle flexibility is an important parameter in determining cell uptake and tumor accumulation, thus modulating therapeutic efficiency in cancer treatment. Herein, we successfully prepared CuS-embedded human serum albumin hollow nanocapsules (denoted CuS/HSA) by a hard-core-assisted layer-by-layer coating approach. This approach afforded CuS/HSA hollow nanocapsules with controllable shell thickness, tunable flexibility, uniform size (272.9 nm), a large hollow cavity, peroxidase-like activity, excellent photothermal conversion ability, and a high tetra-(4-aminophenyl) porphyrin (TAPP) loading capacity (27.3 wt%). The peroxidase-like activity of the CuS nanoparticles enabled them to overcome tumor hypoxia and augment the sonodynamic therapeutic (SDT) effects and photothermal conversion ability for photothermal therapy (PTT). experiments showed that the CuS/HSA-TAPP hollow nanocapsules efficiently induced cancer cell apoptosis under US irradiation and cancer cell ablation under laser irradiation, thus facilitating synergistic SDT and PTT. Importantly, the flexibility of the CuS/HSA hollow nanocapsules resulted in significantly enhanced cellular internalization and a longer mean residence time (131.3 h) than their solid counterparts (21.0 h). In a breast tumor model, the flexible CuS/HSA hollow nanocapsules exhibited high tumor accumulation of up to 27.1%. experiments demonstrated that the flexible CuS/HSA-TAPP hollow nanocapsules effectively eliminated breast tumors the synergistic effect of SDT and PTT.
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http://dx.doi.org/10.1039/d2nr00258bDOI Listing
July 2022

Immunogenicity and Safety of an Inactivated Enterovirus 71 Vaccine Administered Simultaneously with Hepatitis B Virus Vaccine, Group A Meningococcal Polysaccharide Vaccine, Measles-Rubella Combined Vaccine and Japanese Encephalitis Vaccine: A Multi-Center, Randomized, Controlled Clinical Trial in China.

Vaccines (Basel) 2022 Jun 2;10(6). Epub 2022 Jun 2.

Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China.

Background: The aim of this study was to investigate the immunogenicity and safety of the enterovirus 71 vaccine (EV71 vaccine) administered alone or simultaneously.

Methods: A multi-center, open-label, randomized controlled trial was performed involving 1080 healthy infants aged 6 months or 8 months from Shandong, Shanxi, Shaanxi, and Hunan provinces. These infants were divided into four simultaneous administration groups and EV71 vaccine separate administration group. Blood samples were collected from the infants before the first vaccination and after the completion of the vaccination. This trial was registered in the Clinical Trials Registry (NCT03519568).

Results: A total of 895 were included in the per-protocol analysis. The seroconversion rates of antibodies against EV71 in four simultaneous administration groups (98.44% (189/192), 94.57% (122/129), 99.47% (187/188) and 98.45% (190/193)) were non-inferior to EV71 vaccine separate administration group (97.93% [189/193]) respectively. Fever was the most common adverse event, the pairwise comparison tests showed no difference in the incidence rate of solicited, systemic or local adverse events. Three serious adverse events related to the vaccination were reported.

Conclusions: The evidence of immunogenicity and safety supports that the EV71 vaccine administered simultaneously with vaccines need to be administered during the same period of time recommended in China.
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http://dx.doi.org/10.3390/vaccines10060895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9230521PMC
June 2022

iTRAQ proteomic analysis of the inhibitory effect of 1,6-O,O-diacetylbritannilactone on the plant pathogenic oomycete Phytophthora capsici.

Pestic Biochem Physiol 2022 Jun 16;184:105125. Epub 2022 May 16.

College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China; Henan Key Laboratory of Creation and Application of New Pesticide, Henan Agricultural University, No. 63, Agricultural Road, Zhengzhou 450002, Henan, China; Henan Research Center of Green Pesticide Engineering and Technology, Henan Agricultural University, Zhengzhou 450002, Henan, China. Electronic address:

Phytophthora capsici is a highly destructive oomycete of vegetables; its management is challenging due to its broad host range, rapid dispersion, resilient spores and severe fungicide resistance. Identifying an effective alternative fungicide is important for the control of P. capsici. 1,6-O,O-diacetylbritannilactone (ABLOO), one of the secondary metabolites of Inula Britannica, showed a favorable inhibitory activity against P. capsici at different developmental stages, with a sensitivity order as follows: sporangia formation (30.45 mg/L) > zoospore discharge (77.69 mg/L) > mycelial growth (93.18 mg/L) > cystospore germination (591.48 mg/L). To investigate the mode of action of ABLOO in P. capsici, iTRAQ-based quantitative proteomic analysis was performed by comparing the expression levels of proteins in the control and ABLOO-treated (400 mg/L, inhibition rate of 80%) mycelial groups. A total of 65 downregulated and 75 upregulated proteins were identified in the proteomic analysis. Functional enrichment analyses showed that proteins with transmembrane transport activity were significantly inhibited, while proteins involved in energy production were significantly increased, including proteins involved in ubiquinone and other terpenoid-quinone biosynthesis, oxidative phosphorylation, and glycolysis/gluconeogenesis. The morphological results indicated that ABLOO treatment could decrease the thickness of the cell walls of P. capsici mycelia. Correspondingly, biochemical results showed that ABLOO treatment reduced the β-1,3-glucan contents (the key component of the cell wall of P. capsici) and increased the cell membrane permeability of P. capsici. ABLOO may exhibit antioomycete activity by destroying the cell membrane of P. capsici. This study provides new evidence regarding the inhibitory mechanisms of ABLOO against P. capsici.
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http://dx.doi.org/10.1016/j.pestbp.2022.105125DOI Listing
June 2022

Patent Foramen Ovale Closure for Treating Migraine: A Meta-Analysis.

J Interv Cardiol 2022 2;2022:6456272. Epub 2022 Feb 2.

Department of Neurology, West China Hospital, Sichuan University, Wai Nan Guo Xue Lane 37, Chengdu 610041, Sichuan, China.

Background: Observational studies have shown percutaneous patent foramen ovale (PFO) closure to be a safe means of reducing the frequency and duration of migraine.

Objective: This study evaluated the efficacy and safety of PFO closure in patients with migraine using evidence-based medicine.

Methods: The Pubmed (MEDLINE), Embase, and Cochrane Library databases were searched for randomized controlled trials (RCTs), cohort studies, and retrospective case series from January 1, 2001, to February 30, 2021. The Jadad scale and R 4.1.0 software were used to assess the quality of the literature and meta-analysis, respectively.

Results: In total, three randomized controlled trials, one pooled study, and eight retrospective case series including 1,165 participants were included in the meta-analysis. Compared with control intervention in migraine, PFO closure could significantly reduce headache frequency (OR = 1.5698, 95% CI: 1.0465-2.3548, =0.0293) and monthly migraine attacks and monthly migraine days (OR = 0.2594, 95% CI: 0.0790-0.4398, =0.0048). Subgroup analysis of patients who all completed PFO surgery showed resolution of migraine headache for migraines with aura (OR = 1.5856, 95% CI: 1.0665-2.3575, =0.0227).

Conclusions: Treatment with PFO closure could reduce the frequency of headaches and monthly migraine days and is an efficient treatment for migraine attacks with aura.
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http://dx.doi.org/10.1155/2022/6456272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8828350PMC
February 2022

Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer.

J Nanobiotechnology 2021 Oct 29;19(1):347. Epub 2021 Oct 29.

The Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, 453003, Xinxiang, China.

Background: Colorectal cancer is known to be resistant to immune checkpoint blockade (ICB) therapy. Sonodynamic therapy (SDT) has been reported to improve the efficacy of immunotherapy by inducing immunogenic cell death (ICD) of cancer. However, the SDT efficacy is extremely limited by Nrf2-based natural redox balance regulation pathway in cancer cells in response to the increased contents of reactive oxygen species (ROS). Nuclear-targeting strategy has shown unique advantages in tumor therapy by directly destroying the DNA. Thus it can be seen that Nrf2-siRNA augmented nuclear-targeting SDT could boost ICB therapy against colorectal cancer.

Results: The nuclear-targeting delivery system [email protected] (TIR was the abbreviation of assembled TAT-IR780) with great gene carrier capacity and smaller diameter (< 60 nm) was designed to achieve the gene augmented nuclear-targeting SDT facilitating the anti-PD-L1 (programmed cell death-ligand-1) therapy against colorectal cancer. In CT26 cells, [email protected] successfully delivered IR780 (the fluorescent dye used as sonosensitizer) into cell nucleus and Nrf2-siRNA into cytoplasm. Under US (utrasound) irradiation, [email protected] notably increased the cytotoxicity and apoptosis-inducing activity of SDT through down-regulating the Nrf2, directly damaging the DNA, activating mitochondrial apoptotic pathway while remarkably inducing ICD of CT26 cells. In CT26 tumor-bearing mice, [email protected] mediated gene enhanced nuclear-targeting SDT greatly inhibited tumor growth, noticeably increased the T cell infiltration and boosted PPA-1 peptide-based anti-PD-L1 therapy to ablate the primary CT26 tumors and suppress the intestinal metastases.

Conclusions: All results demonstrate that [email protected] under US irradiation can efficiently inhibit the tumor progression toward colorectal CT26 cancer in vitro and in vivo by its mediated gene augmented nuclear-targeting sonodynamic therapy. Through fully relieving the immunosuppressive microenvironment of colorectal cancer by this treatment, this nanoplatform provides a new synergistic strategy for enhancing the anti-PD-L1 therapy to ablate colorectal cancer and inhibit its metastasis.
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http://dx.doi.org/10.1186/s12951-021-01094-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555306PMC
October 2021

Trend of Sudden Unexpected Death in Epilepsy Incidence Rate in Rural West China.

Front Neurol 2021 24;12:735201. Epub 2021 Sep 24.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

To explore the trend of sudden unexpected death in epilepsy (SUDEP) incidence rate over time in rural west China. We scanned probable SUDEP patients from the epilepsy program between 2010 and 2019 in rural West China and performed a verbal autopsy for each eligible patient. We calculated the crude and sex-adjusted incidence rate of SUDEP per person-year over a calendar year and the year of follow-up. We calculated the incidence rate ratio with the Poisson model in STATA 12.0 and calculated the annual percentage change (APC) and average annual percentage change in Joinpoint Trend Analysis Software 4.8.0.1 to analyze the trend of SUDEP incidence rate. In 2010-2019, 44 probable SUDEPs were identified from 10,128 patients with a total person-year of 31,347. The crude and sex-adjusted incidence rates of SUDEP were 1.40 and 1.45%0. Twenty-five (56.8%) of the 44 probable SUDEPs had no generalized tonic-clonic seizure 3 months before their death. The incidence of probable SUDEP decreased significantly in the calendar year [APC = -11.7, 95% confidence interval (CI): -21.7 to -0.3] and in time of follow-up (average annual percentage change = -21.2, 95% CI: -34.3 to -5.4). Comparing the first 5 years in follow-up with the subsequent 3 years, the incidence rate of SUDEP decreased significantly (estimated incidence rate ratio = 0.4, 95% CI: 0.2 to 0.8). SUDEP happened to 1.4 cases per thousand patient-years in convulsive epilepsy in rural west China between 2010 and 2019. The incidence rate of SUDEP presented a downward trend over the time of follow-up.
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http://dx.doi.org/10.3389/fneur.2021.735201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498108PMC
September 2021

An Artificial Neural Network Prediction Model for Posttraumatic Epilepsy: Retrospective Cohort Study.

J Med Internet Res 2021 08 19;23(8):e25090. Epub 2021 Aug 19.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Background: Posttraumatic epilepsy (PTE) is a common sequela after traumatic brain injury (TBI), and identifying high-risk patients with PTE is necessary for their better treatment. Although artificial neural network (ANN) prediction models have been reported and are superior to traditional models, the ANN prediction model for PTE is lacking.

Objective: We aim to train and validate an ANN model to anticipate the risks of PTE.

Methods: The training cohort was TBI patients registered at West China Hospital. We used a 5-fold cross-validation approach to train and test the ANN model to avoid overfitting; 21 independent variables were used as input neurons in the ANN models, using a back-propagation algorithm to minimize the loss function. Finally, we obtained sensitivity, specificity, and accuracy of each ANN model from the 5 rounds of cross-validation and compared the accuracy with a nomogram prediction model built in our previous work based on the same population. In addition, we evaluated the performance of the model using patients registered at Chengdu Shang Jin Nan Fu Hospital (testing cohort 1) and Sichuan Provincial People's Hospital (testing cohort 2) between January 1, 2013, and March 1, 2015.

Results: For the training cohort, we enrolled 1301 TBI patients from January 1, 2011, to December 31, 2017. The prevalence of PTE was 12.8% (166/1301, 95% CI 10.9%-14.6%). Of the TBI patients registered in testing cohort 1, PTE prevalence was 10.5% (44/421, 95% CI 7.5%-13.4%). Of the TBI patients registered in testing cohort 2, PTE prevalence was 6.1% (25/413, 95% CI 3.7%-8.4%). The results of the ANN model show that, the area under the receiver operating characteristic curve in the training cohort was 0.907 (95% CI 0.889-0.924), testing cohort 1 was 0.867 (95% CI 0.842-0.893), and testing cohort 2 was 0.859 (95% CI 0.826-0.890). Second, the average accuracy of the training cohort was 0.557 (95% CI 0.510-0.620), with 0.470 (95% CI 0.414-0.526) in testing cohort 1 and 0.344 (95% CI 0.287-0.401) in testing cohort 2. In addition, sensitivity, specificity, positive predictive values and negative predictors in the training cohort (testing cohort 1 and testing cohort 2) were 0.80 (0.83 and 0.80), 0.86 (0.80 and 0.84), 91% (85% and 78%), and 86% (80% and 83%), respectively. When calibrating this ANN model, Brier scored 0.121 in testing cohort 1 and 0.127 in testing cohort 2. Compared with the nomogram model, the ANN prediction model had a higher accuracy (P=.01).

Conclusions: This study shows that the ANN model can predict the risk of PTE and is superior to the risk estimated based on traditional statistical methods. However, the calibration of the model is a bit poor, and we need to calibrate it on a large sample size set and further improve the model.
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http://dx.doi.org/10.2196/25090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414301PMC
August 2021

Brain function and network features in patients with chronic epilepsy before and after antiseizure medication withdrawal.

Epilepsy Res 2021 10 14;176:106740. Epub 2021 Aug 14.

Epilepsy Center, Department of Neurology, West China Hospital, Sichuan University, No. 37, Guoxue Road, Chengdu, 610041, Sichuan Province, China. Electronic address:

Objectives: A considerable proportion of epilepsy patients who achieved long-term seizure freedom with standardized treatment of antiseizure medication will attempt to withdraw medications. Epilepsy is currently considered as a network disease, however, the characteristics of brain function and neural network before and after medication withdrawal remain to be discovered.

Methods: Resting-state functional magnetic resonance imaging was obtained for 32 healthy controls, 32 seizure-free patients initiating medication tapering (PG1 group), and 16 seizure-free patients that had completely discontinued medications (PG2 group). Amplitude of low-frequency fluctuation and regional homogeneity were calculated to measure local functional activity. Global and nodal metrics of small-world network were calculated based on Graph theory. One-way analysis of variance was applied to analyze intergroup difference, withpost hoc analysis being conducted for each pair of groups.

Results: Sex, age at scanning and other clinical variables showed no significant difference between groups. As compared to control, the amplitude of low-frequency fluctuation, regional homogeneity or nodal metrics of neural network in some brain areas were abnormal in the PG1 or PG2 group; when compared between patient groups, significant between-group differences were also found in the amplitude of low-frequency fluctuation, regional homogeneity or nodal metrics. But, the global metrics of neural network showed no differences among groups.

Conclusions: The global metrics of patients with long-term seizure freedom were normal either before or after antiseizure medication withdrawal, while the local functional activity and nodal metrics in some brain areas were abnormal and differed between before and after antiseizure medication withdrawal.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106740DOI Listing
October 2021

Incidence rate and risk factors of status epilepticus after stroke.

Seizure 2021 Oct 31;91:491-498. Epub 2021 Jul 31.

Department of Neurology, West China Hospital, Sichuan University, Wai Nan Guo Xue Lane 37 #, Chengdu, Sichuan 610041, China. Electronic address:

Purpose To evaluate the incidence rate and risk factors for status epilepticus (SE) after stroke (PSSE), including ischaemic stroke (IS), intracerebral hemorrhage (ICH), and subarachnoid hemorrhage (SAH). Methods A meta-analysis was performed using relevant research from databases such as PubMed, Embase, Cochrane Online Library, and Clinicaltrials.gov. The quality of the studies was evaluated by using the quality evaluation criteria of the Agency for Healthcare Research and Quality (AHRQ). All data were pooled by STATA 12.0 software for meta-analysis. Results The review considered 1650 articles, and 17 articles with 2821 instances of SE among 1088087 instances of stroke were included. The incidence rate of SE after stroke was 6.90 per 1000 total strokes (95% CI: 5.58-8.22). By subgroup analysis of SE, the rates were 33.85‰ (95% CI: 13.77-53.94) for non-convulsive status epilepticus (NCSE) and 2.42‰ (95%CI: 1.66-3.19) for generalized convulsive status epilepticus (GCSE). Age, sex, and presence of atrial fibrillation showed no significant difference between the SE group and the non-SE group after stroke. Hypertension and diabetes are associated with a decreased rate of SE. However, African American race, alcohol abuse, and renal disease are associated with an increased rate of SE. Significance There were approximately 6.9 patients with status epilepticus per 1000 strokes. NCSE is more common after stroke and needs more attention. African American race, alcohol abuse and renal disease may be risk factors for PSSE.
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http://dx.doi.org/10.1016/j.seizure.2021.07.029DOI Listing
October 2021

Functional connectivity of the thalamocortical circuit in patients with seizure relapse after antiseizure medication withdrawal.

Epilepsia 2021 10 3;62(10):2463-2473. Epub 2021 Aug 3.

Epilepsy Center, Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Objective: To characterize the features of thalamocortical functional connectivity during seizure recurrence at the time of antiseizure medication (ASM) withdrawal.

Methods: Patients with chronic epilepsy who attempted to discontinue medications were prospectively registered and followed up; 19 patients remained seizure-free (SF-group), 18 patients had seizure relapses (SR-group) after ASM withdrawal, and 28 healthy controls were recruited. Resting-state functional magnetic resonance imaging was performed before ASM withdrawal. Thalamus subdivisions were set as seeds to calculate voxelwise functional connectivity. Partial correlation analysis between functional connectivity and clinical variables was performed. A support vector machine was used to assess the predictive ability of the specific functional connectivity for seizure relapse.

Results: The within-group comparison indicated that the SR-group had more extensive functional connectivity than the SF-group; the left inferior pulvinar, left medial pulvinar, and right anterior pulvinar showed a significantly stronger functional connection with the precuneus in the SR-group than in the SF-group (Gaussian random field correction, voxel-level p < .001 and cluster-level p < .05). In the SR-group, a positive correlation was found between the left inferior pulvinar-precuneus connectivity and the active period (r = .46, p = .05), seizure-free period (r = .67, p = .002), and disease duration (r = .53, p = .02), and between the left medial pulvinar-precuneus connectivity and the seizure-free period (r = .58, p = .01). The combination of these thalamocortical connections showed a high predictive ability, with an area under the curve of .92 and accuracy of .90 (p = .01).

Significance: This study determined distinct features of thalamocortical functional connectivity at the time of ASM withdrawal in patients with and without seizure relapse, showing a potential for predicting seizure outcomes following ASM withdrawal.
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http://dx.doi.org/10.1111/epi.17014DOI Listing
October 2021

Renewable fatty acid ester production in Clostridium.

Nat Commun 2021 07 16;12(1):4368. Epub 2021 Jul 16.

Department of Biosystems Engineering, Auburn University, Auburn, AL, USA.

Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L butyl acetate and 1.6 g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses.
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http://dx.doi.org/10.1038/s41467-021-24038-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285483PMC
July 2021

Perceived Stress and Internet Addiction Among Chinese College Students: Mediating Effect of Procrastination and Moderating Effect of Flow.

Front Psychol 2021 28;12:632461. Epub 2021 Jun 28.

Department of Psychological Health Education, Normal College, Qingdao University, Qingdao, China.

Perceived stress, Internet addiction and procrastination are common issues among college students. Based on the Compensatory Internet Use (CIU) model and emotion regulation theory, this study aims to explore two possible mechanisms for the connection between perceived stress and Internet addiction: mediating effect of procrastination and moderating effect of flow experience on the Internet. Cross-sectional design and questionnaire survey were used in this study. Data were collected from 446 college students who voluntarily completed self-reporting of perceived stress, internet addiction, procrastination and flow. Potential relationship structure and moderation model between variables was calculated during the process. The results revealed that there were significant associations among perceived stress, Internet addiction, procrastination and flow. The results also showed that procrastination plays a mediating role between perceptual stress and Internet addiction, flow plays a moderating role between them. The results emphasized the importance of the intention behind college students' overuse of the Internet. These results also provided a perspective of finding the possible causes of Internet addiction in college students, that is, individuals use the Internet to avoid stress and procrastinate, and the mobile experience on the Internet also affects the process.
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http://dx.doi.org/10.3389/fpsyg.2021.632461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273309PMC
June 2021

Association of genetic variants of FBXO32 and FOXO6 in the FOXO pathway with breast cancer risk.

Mol Carcinog 2021 10 1;60(10):661-670. Epub 2021 Jul 1.

Duke Cancer Institute, Duke University Medical Center, Durham, North Carolina, USA.

Forkhead box class O (FOXO) transcription factors play a pivotal role in regulating a variety of biological processes, including organismal development, cell signaling, cell metabolism, and tumorigenesis. Therefore, we hypothesize that genetic variants in FOXO pathway genes are associated with breast cancer (BC) risk. To test this hypothesis, we conducted a large meta-analysis using 14 published genome-wide association study (GWAS) data sets in the Discovery, Biology, and Risk of Inherited Variants in Breast Cancer (DRIVE) study. We assessed associations between 5214 (365 genotyped in DRIVE and 4849 imputed) common single-nucleotide polymorphisms (SNPs) in 55 FOXO pathway genes and BC risk. After multiple comparison corrections by the Bayesian false-discovery probability method, we found five SNPs to be significantly associated with BC risk. In stepwise multivariate logistic regression analysis with adjustment for age, principal components, and previously published SNPs in the same data set, three independent SNPs (i.e., FBXO32 rs10093411 A>G, FOXO6 rs61229336 C>T, and FBXO32 rs62521280 C>T) remained to be significantly associated with BC risk (p = 0.0008, 0.0011, and 0.0017, respectively). Additional expression quantitative trait loci analysis revealed that the FBXO32 rs62521280 T allele was associated with decreased messenger RNA (mRNA) expression levels in breast tissue, while the FOXO6 rs61229336 T allele was found to be associated with decreased mRNA expression levels in the whole blood cells. Once replicated by other investigators, these genetic variants may serve as new biomarkers for BC risk.
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http://dx.doi.org/10.1002/mc.23331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475729PMC
October 2021

Brain functional and structural characteristics of patients with seizure recurrence following drug withdrawal.

Neuroradiology 2021 Dec 1;63(12):2087-2097. Epub 2021 Jul 1.

From the Epilepsy Center, Department of Neurology, West China Hospital, Sichuan University, No. 37, Guoxue Road, Chengdu, 610041, Sichuan Province, China.

Purpose: We aimed to analyze the characteristics of brain function and microstructure linked to epilepsy relapse after drug withdrawal in patients with focal epilepsy.

Methods: Resting-state functional magnetic resonance imaging and high-resolution T1-weighted images were acquired within 1 month prior to drug withdrawal from 15 patients who did not have epilepsy relapse (PER - group) and 16 patients who subsequently had epilepsy relapse (PER + group). Additionally, 23 healthy participants undergoing the same scanning protocol were included as controls. Fractional amplitude of low-frequency fluctuation (fALFF) and gray matter density (GMD) were compared among groups. Subgroup and correlation analyses were also performed.

Results: There were no significant differences in fALFF between patient groups, but the PER + group showed lower GMD in the bilateral calcarine, left precuneus, and right superior temporal gyrus than the PER - group (Gaussian random field correction, voxel-level P < 0.001 and cluster-level P < 0.05). Both increased seizure number and polytherapy were associated with lower GMD; also, patients using other antiseizure medications showed lower GMD than those using only levetiracetam (Gaussian random field correction, voxel-level P < 0.001, and cluster-level P < 0.05). The active period and disease duration showed both positive and negative correlations with GMD, while the seizure-free period mainly showed positive correlations with GMD (uncorrected, P < 0.001).

Conclusion: Gray matter microstructure, but not local functional activity, showed distinct characteristics between patients with and without epilepsy relapse and may serve as a potential biomarker for predicting seizure recurrence upon drug withdrawal.
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http://dx.doi.org/10.1007/s00234-021-02755-2DOI Listing
December 2021

[Treatment of unstable fresh thoracolumbar burst fracture by over-bending rod reduction and fixation technique via posterior approach].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Apr;35(4):458-463

Department of Spine Surgery, Luohe Central Hospital, Luohe Henan, 462000, P.R.China.

Objective: To investigate the efficacy and safety of over-bending rod reduction and fixation technique via posterior approach in the treatment of unstable fresh thoracolumbar burst fracture.

Methods: A clinical data of 27 patients with unstable fresh thoracolumbar burst fracture, who were met the inclusive criteria and admitted between January 2018 and October 2019, was retrospectively analyzed. There were 15 males and 12 females with an average age of 41.8 years (range, 26-64 years). The fractures were caused by falling from height in 14 cases, traffic accident in 8 cases, and crushing by a heavy objective in 5 cases. The interval between injury and operation was 1-7 days (mean, 3.2 days). The injured fracture was located at T in 1 case, T in 3 cases, T in 6 cases, L in 7 cases, L in 7 cases, and L in 3 cases. According to AO classification, there were 11 cases of type A3, 7 cases of type B, and 9 cases of type C. Neurological function was rated as grade A in 3 cases, grade B in 7 cases, grade C in 5 cases, and grade D in 12 cases according to the American Spinal Injury Association (ASIA) grading. All cases were treated by over-bending rod reduction and fixation technique via posterior approach, and 16 cases were combined with limited fenestration decompression. The evaluation indicators consisted of operation time, intraoperative blood loss, the compression ratio of the anterior vertebral height, the invasion rate of the injured vertebra into the spinal canal, the Cobb angle of segmental kyphosis, visual analogue scale (VAS) score, and Oswestry Disability Index (ODI).

Results: The operation time was 67-128 minutes (mean, 81.6 minutes), and the intraoperative blood loss was 105-295 mL (mean, 210 mL). All patients were followed up 12-23 months (mean, 17.2 months). A total of 178 pedicle screws were implanted during operation, and the accuracy of the implantation was 98.9% (176/178). The compression ratios of the anterior vertebral height at the early postoperatively and last follow-up were significantly increased when compared with preoperative one ( <0.05), and the invasion rate of the injured vertebra into the spinal canal, Cobb angle, VAS score, and ODI were significantly lower than those preoperatively ( <0.05). Except that the ODI at last follow-up was significantly lower than that of the early postoperative period ( <0.05), there was no significant difference between the last follow-up and the early postoperative period for other indicators ( >0.05). At last follow-up, the neurological function was rated as grade A in 1 case, grade B in 2 cases, grade C in 4 cases, grade D in 9 cases, and grade E in 11 cases according to the ASIA grading, showing significant difference when compared with that before operation ( =-3.446, =0.001).

Conclusion: Over-bending rod reduction and fixation technique can effectively restore vertebral height, reset the invaded vertebral block, and selectively perform limited decompression and posterolateral bone grafting to ensure the completeness of intravertebral decompression and stability, which is one of the effective methods to treat unstable fresh thoracolumbar burst vertebral fracture.
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http://dx.doi.org/10.7507/1002-1892.202011063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171619PMC
April 2021

Association of genetic variants of and in the peroxisome pathway with cutaneous melanoma-specific survival.

Ann Transl Med 2021 Mar;9(5):396

Duke Cancer Institute, Duke University Medical Center, Durham, NC, USA.

Background: Peroxisomes are ubiquitous and dynamic organelles that are involved in the metabolism of reactive oxygen species (ROS) and lipids. However, whether genetic variants in the peroxisome pathway genes are associated with survival in patients with melanoma has not been established. Therefore, our aim was to identify additional genetic variants in the peroxisome pathway that may provide new prognostic biomarkers for cutaneous melanoma (CM).

Methods: We assessed the associations between 8,397 common single-nucleotide polymorphisms (SNPs) in 88 peroxisome pathway genes and CM disease-specific survival (CMSS) in a two-stage analysis. For the discovery, we extracted the data from a published genome-wide association study from The University of Texas MD Anderson Cancer Center (MDACC). We then replicated the results in another dataset from the Nurse Health Study (NHS)/Health Professionals Follow-up Study (HPFS).

Results: Overall, 95 (11.1%) patients in the MDACC dataset and 48 (11.7%) patients in the NHS/HPFS dataset died of CM. We found 27 significant SNPs in the peroxisome pathway genes to be associated with CMSS in both datasets after multiple comparison correction using the Bayesian false-discovery probability method. In stepwise Cox proportional hazards regression analysis, with adjustment for other covariates and previously published SNPs in the MDACC dataset, we identified 2 independent SNPs ( rs567403 C>G and rs7969508 A>G) that predicted CMSS (P=0.003 and 0.031, respectively, in an additive genetic model). The expression quantitative trait loci analysis further revealed that the rs567403 GG and rs7969508 GG genotypes were associated with increased and decreased levels of mRNA expression of their genes, respectively.

Conclusions: Once our findings are replicated by other investigators, these genetic variants may serve as novel biomarkers for the prediction of survival in patients with CM.
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http://dx.doi.org/10.21037/atm-20-2117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033299PMC
March 2021

Glycine max NNL1 restricts symbiotic compatibility with widely distributed bradyrhizobia via root hair infection.

Nat Plants 2021 01 15;7(1):73-86. Epub 2021 Jan 15.

Center of Integrative Biology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.

Symbiosis between soybean (Glycine max) and rhizobia is essential for efficient nitrogen fixation. Rhizobial effectors secreted through the type-III secretion system are key for mediating the interactions between plants and rhizobia, but the molecular mechanism remains largely unknown. Here, our genome-wide association study for nodule number identified G. max Nodule Number Locus 1 (GmNNL1), which encodes a new R protein. GmNNL1 directly interacts with the nodulation outer protein P (NopP) effector from Bradyrhizobium USDA110 to trigger immunity and inhibit nodulation through root hair infection. The insertion of a 179 bp short interspersed nuclear element (SINE)-like transposon into GmNNL1 leads to the loss of function of GmNNL1, enabling bradyrhizobia to successfully nodulate soybeans through the root hair infection route and enhancing nitrogen fixation. Our findings provide important insights into the coevolution of soybean-bradyrhizobia compatibility and offer a way to design new legume-rhizobia interactions for efficient symbiotic nitrogen fixation.
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http://dx.doi.org/10.1038/s41477-020-00832-7DOI Listing
January 2021

Metabolomics Analysis of the Effect of Glutamic Acid on Monacolin K Synthesis in .

Front Microbiol 2020 17;11:610471. Epub 2020 Dec 17.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing, China.

Monacolin K is a secondary metabolite produced by with beneficial effects on health, including the ability to lower cholesterol. We previously showed that the yield of monacolin K was significantly improved when glutamic acid was added to the fermentation broth of M1. In this study, we analyzed in media with and without glutamic acid supplementation using a metabolomic profiling approach to identify key metabolites and metabolic pathway differences. A total of 817 differentially expressed metabolites were identified between the two fermentation broths on day 8 of fermentation. Pathway analysis of these metabolites using the KEGG database indicated overrepresentation of the citric acid cycle; biotin metabolism; and alanine, aspartate, and glutamate metabolic pathways. Six differentially expressed metabolites were found to be related to the citric acid cycle. The effect of citric acid as an exogenous additive on the synthesis of monacolin K was examined. These results provide technical support and a theoretical basis for further studies of the metabolic regulatory mechanisms underlying the beneficial effects of monacolin K and medium optimization, as well as genetic engineering of M1 for efficient monacolin K production.
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http://dx.doi.org/10.3389/fmicb.2020.610471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773642PMC
December 2020

GSK3-mediated stress signaling inhibits legume-rhizobium symbiosis by phosphorylating GmNSP1 in soybean.

Mol Plant 2021 03 21;14(3):488-502. Epub 2020 Dec 21.

State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475001, China. Electronic address:

Legumes establish symbiotic associations with rhizobia for biological nitrogen fixation. This process is highly regulated by various abiotic stresses, but the underlying genetic and molecular mechanisms remain largely unknown. In this study, we discovered that the glycogen synthase kinase 3 (GSK3)-like kinase, GmSK2-8, plays an important role in inhibiting symbiotic signaling and nodule formation in soybean (Glycine max) under salt stress. We found that GmSK2-8 is strongly induced in soybean under high-salt conditions, while GmSK2-8 could interact with two G. max Nodulation Signaling Pathway 1 (GmNSP1) proteins, GmNSP1a and GmNSP1b; these key transcription factors are essential for rhizobial infection, nodule initiation, and symbiotic gene expression in soybean. Furthermore, we demonstrated that GmSK2-8 phosphorylates the LHRI domain of GmNSP1a, inhibits its binding to the promoters of symbiotic genes, and thus suppresses nodule formation under salt stress. Knockdown of GmSK2-8 and its close homologs also resulted in reduced plant sensitivity to salt stress during nodule formation. Taken together, our findings indicate that GSK3-like kinases directly regulate the activities of GmNSP1s to mediate salt-inhibited legume-rhizobium symbiosis, providing novel targets for improving symbiotic nitrogen fixation under environmental stress conditions in soybean and possibly other legumes.
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http://dx.doi.org/10.1016/j.molp.2020.12.015DOI Listing
March 2021

Microstructural features of the cerebral cortex: Implications for predicting epilepsy relapse after drug withdrawal.

Brain Res 2021 01 6;1751:147200. Epub 2020 Nov 6.

From the Epilepsy Center, Department of Neurology, West China Hospital, Sichuan University, No. 37, Guoxue Road, Chengdu 610041, Sichuan Province, China. Electronic address:

A considerable portion of patients with well-controlled seizures and visually normal brain structures experience seizure recurrence after anti-seizure medication is withdrawn. Microstructural abnormalities of the cortex may play an essential role in epilepsy relapse. Patients with idiopathic/cryptogenic epilepsy were registered. At the follow-up endpoint, 18 patients with relapse (PR+ group), 20 patients without relapse (PR- group), and 30 healthy controls were included. High-resolution T1-weighted images were obtained at the time of drug withdrawal. Microstructural features including cortical thickness, surface area, cortical volume and mean curvature in 68 cortical areas were calculated. A general linear model was applied to investigate intergroup differences, and then post hoc analysis was performed. Additionally, factor analysis was conducted to extract components from imaging measures showing a difference between PR- and PR+ groups, and independent associations between components and epilepsy relapse were assessed using a logistic regression model. Cortical thickness of the left paracentral lobule, left temporal pole and right superior frontal gyrus; surface area of the bilateral lingual gyrus and bilateral pericalcarine cortex; and cortical volume of the bilateral pericalcarine cortex had significant intergroup differences (false discovery rate correction, P < 0.05). All measures, except for cortical thickness of the left temporal pole, showed differences between PR- and PR+ groups. Two dominant components were extracted from these measures, and both were independently associated with epilepsy relapse. In conclusion, epilepsy patients with relapse presented distinct microstructural features of cortex at the time of drug withdrawal, which may serve as a potential biomarker for predicting seizure recurrence.
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http://dx.doi.org/10.1016/j.brainres.2020.147200DOI Listing
January 2021

State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks.

Int J Mol Sci 2020 Oct 31;21(21). Epub 2020 Oct 31.

State Key Laboratory of Surface Physics, Multiscale Research Institute of Complex Systems, Department of Physics, Fudan University, Shanghai 200433, China.

Brassinosteroids, the steroid hormones of plants, control physiological and developmental processes through its signaling pathway. The major brassinosteroid signaling network components, from the receptor to transcription factors, have been identified in the past two decades. The development of biotechnologies has driven the identification of novel brassinosteroid signaling components, even revealing several crosstalks between brassinosteroid and other plant signaling pathways. Herein, we would like to summarize the identification and improvement of several representative brassinosteroid signaling components through the development of new technologies, including brassinosteroid-insensitive 1 (BRI1), BRI1-associated kinase 1 (BAK1), BR-insensitive 2 (BIN2), BRI1 kinase inhibitor 1 (BKI1), BRI1-suppressor 1 (BSU1), BR signaling kinases (BSKs), BRI1 ethyl methanesulfonate suppressor 1 (BES1), and brassinazole resistant 1 (BZR1). Furthermore, improvement of BR signaling knowledge, such as the function of BKI1, BES1 and its homologous through clustered regularly interspaced short palindromic repeats (CRISPR), the regulation of BIN2 through single-molecule methods, and the new in vivo interactors of BIN2 identified by proximity labeling are described. Among these technologies, recent advanced methods proximity labeling and single-molecule methods will be reviewed in detail to provide insights to brassinosteroid and other phytohormone signaling pathway studies.
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http://dx.doi.org/10.3390/ijms21218179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662629PMC
October 2020

UAV Autonomous Tracking and Landing Based on Deep Reinforcement Learning Strategy.

Sensors (Basel) 2020 Oct 1;20(19). Epub 2020 Oct 1.

Key Laboratory of Electronics and Information Technology for Space System, National Space Science Center, Chinese Academy of Sciences, Beijing 100190, China.

Unmanned aerial vehicle (UAV) autonomous tracking and landing is playing an increasingly important role in military and civil applications. In particular, machine learning has been successfully introduced to robotics-related tasks. A novel UAV autonomous tracking and landing approach based on a deep reinforcement learning strategy is presented in this paper, with the aim of dealing with the UAV motion control problem in an unpredictable and harsh environment. Instead of building a prior model and inferring the landing actions based on heuristic rules, a model-free method based on a partially observable Markov decision process (POMDP) is proposed. In the POMDP model, the UAV automatically learns the landing maneuver by an end-to-end neural network, which combines the Deep Deterministic Policy Gradients (DDPG) algorithm and heuristic rules. A Modular Open Robots Simulation Engine (MORSE)-based reinforcement learning framework is designed and validated with a continuous UAV tracking and landing task on a randomly moving platform in high sensor noise and intermittent measurements. The simulation results show that when the moving platform is moving in different trajectories, the average landing success rate of the proposed algorithm is about 10% higher than that of the Proportional-Integral-Derivative (PID) method. As an indirect result, a state-of-the-art deep reinforcement learning-based UAV control method is validated, where the UAV can learn the optimal strategy of a continuously autonomous landing and perform properly in a simulation environment.
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http://dx.doi.org/10.3390/s20195630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582896PMC
October 2020

Blood Lead, Systemic Inflammation, and Blood Pressure: Exploring Associations and Mediation Effects in Workers Exposed to Lead.

Biol Trace Elem Res 2021 Jul 21;199(7):2573-2581. Epub 2020 Sep 21.

Department of Occupational & Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.

Relationships of lead exposure with blood pressure and blood lead with inflammation have been previously established yet, but the conclusions are still controversial. The objective of our study was to investigate the role of systemic inflammation in the relationships between blood lead concentration and blood pressure. We quantified the levels of blood lead and white blood cell count in 505 lead-exposed workers with 842 observations. Associations between blood lead, white blood cell count, and blood pressure were evaluated by using linear mixed models. We further performed mediation analysis to investigate the role of white blood cell count in the associations between blood lead concentration and blood pressure. We observed that each 1% increase of blood lead levels was significantly positively associated with a 0.9%, 1.7%, and 1.1% increases in systolic blood pressure, white blood cell count, and blood platelet count, respectively. Also, we found that the levels of white blood cell count were positively correlated with diastolic blood pressure and systolic blood pressure in a dose-response manner. Mediation analysis showed that the levels of white blood cell significantly mediated the associations between concentration of blood lead and systolic blood pressure. Collectively, our findings suggest that blood lead was positively associated with systolic blood pressure and that systemic inflammation might play a key role in this association.
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http://dx.doi.org/10.1007/s12011-020-02397-0DOI Listing
July 2021

Catalog of Lung Cancer Gene Mutations Among Chinese Patients.

Front Oncol 2020 4;10:1251. Epub 2020 Aug 4.

Department of Respiratory and Critical Care, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Detailed catalog of lung cancer-associated gene mutations provides valuable information for lung cancer diagnosis and treatment. In China, there has never been a wide-ranging study cataloging lung cancer-associated gene mutations. This study aims to reveal a comprehensive catalog of lung cancer gene mutations in china, focusing on EGFR, ALK, KRAS, HER2, PIK3CA, MET, BRAF, HRAS, and CTNNB1 as major targets. Additionally, we also aim to correlate smoking history, gender, and age distribution and pathological types with various types of gene mutations. A retrospective data acquisition was conducted spanning 6 years (2013-2018) among all patients who underwent lung cancer surgeries not bronchial or percutaneous lung biopsy at three major tertiary hospitals. Finally, we identified 1,729 patients who matched our inclusion criteria. 1081 patients (62.49%) harbored EGFR mutation. ALK ( = 42, 2.43%), KRAS ( = 201, 11.62%), CTNNB1 ( = 28, 1.62%), BRAF ( = 31, 1.79%), PIK3CA ( = 51, 2.95%), MET ( = 14, 0.81%), HER2 ( = 47, 2.72%), HRAS ( = 3, 0.17%), and other genes( = 232, 13.4%). Females expressed 55.38% vs. males 44.62% mutations. Among subjects with known smoking histories, 32.82% smokers, 67.15% non-smokers were observed. Generally, 51.80% patients were above 60 years vs. 48.20% in younger patients. Pathological types found includes LUADs 71.11%, SQCCs 1.68%, ASC 0.75%, LCC 0.58%, SCC 0.35%, ACC 0.17%, and SC 0.06%, unclear 25.19%. We offer a detailed catalog of the distribution of lung cancer mutations. Showing how gender, smoking history, age, and pathological types are significantly related to the prevalence of lung cancer in China.
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http://dx.doi.org/10.3389/fonc.2020.01251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417348PMC
August 2020

Effectiveness of fire needle combining with moist healing dressing to promote the growth of granulation tissue in chronic wounds: A case report.

Int J Nurs Sci 2020 Jul 30;7(3):386-390. Epub 2020 May 30.

Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

In this case study, we analyzed the wound-healing process of a patient with a chronic wound who underwent fire needle treatment, and we tracked the coverage of granulation tissue and decrease of slough and exudate. An 85-year-old man had repeated right shoulder and back pain, itching, and skin festering for more than 1.5 years. A fire needle was administered combined with moist dressing once every 5 days to promote wound healing. After six rounds of fire needle treatment, granulation tissue formed over the surface of the wound base, the depth of the wound had become shallow, and the wound area was reduced. No complications occurred during the intervention. Fire needle therapy combined with a moist wound-healing dressing can be an effective alternative approach in managing chronic wounds.
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http://dx.doi.org/10.1016/j.ijnss.2020.05.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7424147PMC
July 2020

Variants in , and in vitamin D pathway genes are associated with breast cancer risk: a large-scale analysis of 14 GWASs in the DRIVE study.

Am J Cancer Res 2020 1;10(7):2160-2173. Epub 2020 Jul 1.

Duke Cancer Institute, Duke University Medical Center Durham 27710, NC, USA.

Vitamin D has a potential anticarcinogenic role, possibly through regulation of cell proliferation and differentiation, stimulation of apoptosis, immune modulation and regulation of estrogen receptor levels. Because breast cancer (BC) risk varies among individuals exposed to similar risk factors, we hypothesize that genetic variants in the vitamin D pathway genes are associated with BC risk. To test this hypothesis, we performed a larger meta-analysis using 14 published GWAS datasets in the Discovery, Biology, and Risk of Inherited Variants in Breast Cancer (DRIVE) Study. We assessed associations between 2,994 (237 genotyped in the DRIVE study and 2,757 imputed from the 1000 Genomes Project) single nucleotide polymorphisms (SNPs) in 33 vitamin D pathway genes and BC risk. In unconditional logistic regression analysis, we found 11 noteworthy SNPs to be associated with BC risk after multiple comparison correction by the Bayesian false-discovery probability method (<0.80). In stepwise logistic regression analysis, with adjustment for age, principal components and previously published SNPs in the same study populations, we identified three independent SNPs ( rs1047920 C>T, rs11826 C>T and rs3914238 C>T) to be associated with BC risk ( = 0.0014, 0.0020 and 0.0022, respectively). Additional expression quantitative trait loci analysis revealed that the rs73276407 A allele, in a high LD with the rs1047920 T allele, was associated with decreased mRNA expression levels, while the rs11826 T allele was significantly associated with elevated mRNA expression levels. Once replicated by other investigators and additional mechanistic studies, these genetic variants may serve as new biomarkers for susceptibility to BC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407344PMC
July 2020

Circulating Lymphocyte Subsets Induce Secondary Infection in Acute Pancreatitis.

Front Cell Infect Microbiol 2020 31;10:128. Epub 2020 Mar 31.

Department of Hepatology, The First Hospital of Jilin University, Jilin University, Changchun, China.

Acute pancreatitis (AP) is considered a cascade of immune responses triggered by acinar cell necrosis. AP involves two main processes of systemic inflammatory response syndrome and subsequent compensatory anti-inflammatory response syndrome. Although great efforts have been made regarding AP therapy, the mortality rate of AP remains high. Secondary infection acts a lethal factor in AP. Lymphocytes act as major immune mediators in immune responses in the course of this disease. However, the relationship between lymphocytes and secondary infection in AP is unclear. This review summarizes the variation of lymphocytes and infection in AP. Knowledge of the characterization of circulating lymphocyte abnormalities is relevant for understanding the pathophysiology of AP.
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http://dx.doi.org/10.3389/fcimb.2020.00128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136471PMC
June 2021
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