Publications by authors named "Haihua Wang"

64 Publications

Jiyuan Oridonin A Overcomes Differentiation Blockade in Acute Myeloid Leukemia Cells With MLL Rearrangements Multiple Signaling Pathways.

Front Oncol 2021 26;11:659720. Epub 2021 Mar 26.

Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang, China.

Differentiation therapy with all-trans-retinoic acid (ATRA) in acute promyelocytic leukemia (APL), a subtype of acute myeloid leukemia (AML), has been extremely successful in inducing clinical remission in APL patients. However, the differentiation therapy of ATRA-based treatment has not been effective in other subtypes of AML. In this study, we evaluated a small molecule of -kaurene diterpenoid, Jiyuan oridonin A (JOA), on the differentiation blockade in AML cells with the mixed lineage leukemia (MLL) gene rearrangements (MLLr) in MV4-11, MOLM-13 and THP-1 cells. We found that JOA could significantly inhibit the proliferation of MOLM-13, MV4-11 and THP-1 cells. Moreover, JOA promoted cell differentiation coupled with cell-cycle exit at G0/G1 and inhibited the colony- forming capacity of these cells. We showed that the anti-proliferative effect of JOA attributed to cell differentiation is most likely through the martens tretinoin response up pathway in the MOLM-13 cell line, and the hematopoietic cell lineage pathway by the inhibition of c-KIT expression and cell adhesion pathway in the THP-1 cell line. Our findings suggest that JOA could be a novel therapeutic agent against human MLLr acute myeloid leukemia.
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http://dx.doi.org/10.3389/fonc.2021.659720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033199PMC
March 2021

OGP46 Induces Differentiation of Acute Myeloid Leukemia Cells via Different Optimal Signaling Pathways.

Front Cell Dev Biol 2021 4;9:652972. Epub 2021 Mar 4.

Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John's University, New York, NY, United States.

Acute myelogenous leukemia (AML) is characterized by blockage of cell differentiation leading to the accumulation of immature cells, which is the most prevalent form of acute leukemia in adults. It is well known that all-trans retinoic acid (ATRA) and arsenic trioxide (ATO) are the preferred drugs for acute promyelocytic leukemia (APL). However, they can lead to irreversible resistance which may be responsible for clinical failure after complete remission (CR). Moreover, the differentiation therapy of ATRA-based treatment has not been effective against AML with t(8;21) translocation. Here we aimed to identify the differentiation effect of OGP46 on AML cell lines (HL-60, NB4, and Kasumi-1) and explore its possible mechanisms. We found that OGP46 has significant inhibitory activity against these cells by triggering cell differentiation with cell-cycle exit at G1/G0 and inhibited the colony-formation capacity of the AML cells. It was shown that OGP46 induced the differentiation of NB4 cells via the transcriptional misregulation in cancer signaling pathway by PML-RARα depletion, while it was attributed to the hematopoietic cell lineage and phagosome pathway in Kasumi-1 cells, which are all critical pathways in cell differentiation. These results highlight that OGP46 is an active agent not only in the APL cell line NB4 but also in AML-M2 cell lines, especially Kasumi-1 with t(8;21) translocation. Therefore, OGP46 may be a potential compound for surmounting the differentiation blockage in AML.
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http://dx.doi.org/10.3389/fcell.2021.652972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969801PMC
March 2021

Diverse and abundant antibiotic resistance genes in mangrove area and their relationship with bacterial communities - A study in Hainan Island, China.

Environ Pollut 2021 May 8;276:116704. Epub 2021 Feb 8.

College of Life Science, Hainan Normal University, Haikou, 571158, China.

Antibiotic resistance genes (ARGs) are emerging contaminants in the environment and have been highlighted as a worldwide environmental and health concern. As important participants in the biogeochemical cycles, mangrove ecosystems are subject to various anthropogenic disturbances, and its microbiota may be affected by various contaminants such as ARGs. This study selected 13 transects of mangrove-covered areas in Hainan, China for sediment sample collection. The abundance and diversity of ARGs and mobile genetic elements (MGEs) were investigated using high-throughput quantitative polymerase chain reaction (HT-qPCR), and high-throughput sequencing was used to study microbial structure and diversity. A total of 179 ARGs belonging to 9 ARG types were detected in the study area, and the detection rates of vanXD and vatE-01 were 100%. The abundance of ARGs was 8.30 × 10-6.88 × 10 copies per g sediment (1.27 × 10-3.39 × 10 copies per 16S rRNA gene), which was higher than similar studies, and there were differences in the abundance of ARGs in these sampling transects. The multidrug resistance genes (MRGs) accounted for the highest proportion (69.0%), which indicates that the contamination of ARGs in the study area was very complicated. The ARGs significantly positively correlated with MGEs, which showed that the high level of ARGs was related to its self-enhancement. The dominant bacteria at the genus level were Desulfococcus, Clostridium, Rhodoplanes, Bacillus, Vibrio, Enterococcus, Sedimentibacter, Pseudoalteromonas, Paracoccus, Oscillospira, Mariprofundus, Sulfurimonas, Aminobacterium, and Novosphingobium. There was a significant positive correlation between 133 bacterial genera and some ARGs. Chthoniobacter, Flavisolibacter, Formivibrio, Kaistia, Moryella, MSBL3, Perlucidibaca, and Zhouia were the main potential hosts of ARGs in the sediments of Hainan mangrove area, and many of these bacteria are important participants in biogeochemical cycles. The results contribute to our understanding of the distribution and potential hosts of ARGs and provide a scientific basis for the protection and management of Hainan mangrove ecosystem.
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http://dx.doi.org/10.1016/j.envpol.2021.116704DOI Listing
May 2021

The impact of policy mixes on new energy vehicle diffusion in China.

Clean Technol Environ Policy 2021 Feb 22:1-18. Epub 2021 Feb 22.

Antai College of Economics and Management, Shanghai Jiao Tong University, Shanghai, 200030 China.

Abstract: The Chinese government has instituted a number of policies to actively promote the diffusion of new energy vehicles (NEVs). There is widespread consensus that policy mixes can drive NEV diffusion effectively. To verify this consensus, we construct a two-dimensional framework of NEV policy instruments: producer-orientation versus consumer-orientation instruments, economic in cash versus regulatory instruments and classify NEV policy mixes. Then, we analyse the impact of policy mixes on NEV diffusion among enterprises and consumers by building an evolutionary game model. And according to the concept of stable area in the evolutionary game, we propose a definition of policy effect. The results show: (1) Policy mixes can reduce the saddle point of the auto market game and accelerate the spread of NEVs. In the early stages of the NEV industry, the government should take the policy mix strategy with four policies, and economic in cash instrument should be the main policy. (2) Policy mixes composed of producer-orientation and consumer-orientation instruments have a synergy or complementarity to promote NEV diffusion effectively. (3) With the increasing rate of NEV production and consumption, the government should adopt policy mixes mainly composed of regulatory instruments and rather than economic instruments.

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http://dx.doi.org/10.1007/s10098-021-02040-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897522PMC
February 2021

Emerging forward osmosis and membrane distillation for liquid food concentration: A review.

Compr Rev Food Sci Food Saf 2021 Mar 12;20(2):1910-1936. Epub 2021 Jan 12.

College of Food Science and Engineering, Northwest A&F University, Yangling, China.

As emerging membrane technologies, forward osmosis (FO) and membrane distillation (MD), which work with novel driving forces, show great potential for liquid food concentration, owing to their low fouling propensity and great driving force. In the last decades, they have attracted the attention of food industry scientists in global scope. However, discussions of the FO and MD in liquid food concentration advancement, membrane fouling, and economic assessment have been scant. This review aims to provide an up-to-date knowledge about liquid food concentration by FO and MD. First, we introduce the principle and applications of FO and MD in liquid food concentration, and highlight the effect of process on liquid food composition, membrane fouling mechanism, and strategies for fouling mitigation. Besides, economic assessment of FO and MD processes is reviewed. Moreover, the challenges as well as future prospects of FO and MD applied in liquid food concentration are proposed and discussed. Comparing with conventional membrane-based or thermal-based technologies, FO and MD show outstanding advantages in high concentration rate, good concentrate quality, low fouling propensity, and low cost. Future efforts for liquid food concentration by FO and MD include (1) development of novel FO draw solution (DS); (2) understanding the effects of liquid food complex compositions on membrane fouling in FO and MD concentration process; and (3) fabrication of novel membranes and innovation of membrane module and process configuration for liquid food processing.
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http://dx.doi.org/10.1111/1541-4337.12691DOI Listing
March 2021

Modulators of histone demethylase JMJD1C selectively target leukemic stem cells.

FEBS Open Bio 2021 Jan 16;11(1):265-277. Epub 2020 Dec 16.

Laboratory for Stem Cell and Regenerative Medicine & Clinical Research Center, The Affiliated Hospital of Weifang Medical University, China.

Leukemic stem cells (LSCs) comprise a very rare cell population that results in the development of acute myeloid leukemia. The selective targeting of drivers in LSCs with small molecule inhibitors holds promise for treatment of acute myeloid leukemia. Recently, we reported the identification of inhibitors of the histone lysine demethylase JMJD1C that preferentially kill MLL rearranged acute leukemia cells. Here, we report the identification of jumonji domain modulator #7 (JDM-7). Surface plasmon resonance analysis showed that JDM-7 binds to JMJD1C and its family homolog JMJD1B. JDM-7 did not significantly suppress cell proliferation in liquid cell culture at higher doses, although it led to a significant decrease in semi-solid colony formation experiments at lower concentrations. Moreover, low doses of JDM-7 did not suppress the proliferation of erythroid progenitor cells. We identified that JDM-7 downregulates the LSC self-renewal gene HOXA9 in leukemia cells. We further found that the structure of JDM-7 is similar to that of tadalafil, a drug approved by the US Food and Drug Administration. Molecular docking and surface plasmon resonance analysis showed that tadalafil binds to JMJD1C. Moreover, similar to JDM-7, tadalafil suppressed colony formation of leukemia cells in semi-solid cell culture at a concentration that did not affect primary umbilical cord blood cells. In summary, we have identified JDM-7 and tadalafil as potential JMJD1C modulators that selectively inhibit the growth of LSCs.
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http://dx.doi.org/10.1002/2211-5463.13054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780120PMC
January 2021

CircRNAs and LncRNAs in Osteoporosis.

Differentiation 2020 Nov - Dec;116:16-25. Epub 2020 Oct 28.

Jiangxi University of Science and Technology, Ganzhou, 341000, China; State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha, 410083, China. Electronic address:

Osteoporosis is a systemic bone disease with bone fragility and increased fracture risk. The non-coding RNAs (ncRNAs) have appeared as important regulators of cellular signaling and pertinent human diseases. Studies have demonstrated that circular RNAs (circRNAs) and long non-coding RNAs (lncRNAs) are involved in the progression of osteoporosis through a variety of pathways, and are considered as targets for the prophylaxis and treatment of osteoporosis. Based on an in-depth understanding of their roles and mechanisms in osteoporosis, we summarize the functions and molecular mechanisms of circRNAs and lncRNAs involved in the progression of osteoporosis and provide some new insights for the prognosis, diagnosis and treatment of osteoporosis.
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http://dx.doi.org/10.1016/j.diff.2020.10.002DOI Listing
October 2020

Ultrasensitive Photoelectrochemical Assay for DNA Detection Based on a Novel SnS/CoO Sensitized Structure.

Anal Chem 2020 11 13;92(21):14769-14774. Epub 2020 Oct 13.

Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.

In this work, an ultrasensitive photoelectrochemical (PEC) assay was established for sensitive DNA detection based on a novel SnS/CoO sensitized structure as a photoactive matrix and benzo-4-chlorohexadienone (4-CD) precipitate as a signal quencher. Noticeably, the photoelectric conversion efficiency of the SnS/CoO sensitized structure was dramatically enhanced due to the effective sensitization of CoO toward SnS, thus attaining an intense photocurrent response, which was sixfold higher than that of pristine SnS. Additionally, with the assistance of Nt.BstNBI enzyme-assisted target cycling process, a limited amount of target DNA (a fragment of p53 gene) could be converted into extensive output DNA, which could hybridize with capture DNA to yield abundant DNA duplex for loading mimetic enzyme manganese porphyrin (MnPP). Subsequently, 4-chloro-1-naphthol (4-CN) could be catalyzed to form 4-CD precipitate by MnPP on the modified electrode surface with the existence of HO. Then, the 4-CD precipitate severely hampered electron transfer, causing a prominently diminished photocurrent response for DNA determination. The elaborated PEC assay not only extended the application of SnS in the PEC biosensing field but also manifested a wide linear range of 100 aM to 1 nM with a low detection limit of 30 aM, exhibiting enormous potential for the detection of various biomarkers or other targets in bioanalysis and disease diagnosis fields.
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http://dx.doi.org/10.1021/acs.analchem.0c03497DOI Listing
November 2020

First Report of Causing Leaf Spot on Tartary Buckwheat in China.

Plant Dis 2020 Oct 6. Epub 2020 Oct 6.

Chinese Academy of Agricultural Sciences Institute of Crop Sciences, 200653, No.12 Zhongguancun South St., Haidian District, Beijing, China, 100081;

Buckwheat (Fagopyrum tataricum) is a traditional short-season pseudocereal crop originating in southwest China and is cultivated around the world. Antioxidative substances in buckwheat have been shown to provide many potential cardiovascular health benefits. Between August and November in 2019, a leaf spot was found in several Tartary buckwheat cv. Pinku1 fields in Xiangxiang County, Hunan Province, China. The disease occurred throughout the growth cycle of buckwheat after leaves emerged, and disease incidence was approximately 50 to 60%. Initially infected leaves developed a few round lesions, light yellow to light brown spots. Several days later, lesions began to enlarge with reddish brown borders, and eventually withered and fell off. Thirty lesions (2×2 mm) collected from three locations with ten leaves in each location were sterilized in 70% ethanol for 10 sec, in 2% sodium hypochlorite for 30 sec, rinsed in sterile water for three times, dried on sterilized filter paper, and placed on a potato dextrose PDA with lactic acid (3 ml/L), and incubated at 28°C in the dark for 3 to 5 days. Fungal colonies were initially white and later turned black with the onset ofsporulation. Conidia were single-celled, black, smooth, spherical to subspherical, and measured 9.2 to 15.6 µm long, and 7.1 to 11.6 µm wide (n=30). Each conidium was terminal and borne on a hyaline vesicle at the tip of conidiophores. Morphologically, the fungus was identified as Nigrospora osmanthi (Wang et al. 2017). Identification was confirmed by amplifying and sequencing the ITS region, and translation elongation factor 1-alpha (TEF1-α) and partial beta-tublin (TUB2) genes using primers ITS1/ITS4 (Mills et al. 1992), EF1-728F/EF-2 (Carbone and Kohn 1999; O'Donnell et al. 1998) and Bt-2a/Bt-2b (Glass et al. 1995), respectively. BLAST searches in GenBank indicated the ITS (MT860338), TUB2 (MT882054) and TEF1-α (MT882055) sequences had 99.80%, 99% and 100% similarity to sequences KX986010.1, KY019461.1 and KY019421.1 of Nigrospora osmanthi ex-type strain CGMCC 3.18126, respectively. A neighbor-joining phylogenetic tree constructed using MEGA7.0 with 1,000 bootstraps based on the concatenated nucleotide sequences of the three genes indicated that our isolate was closely related to N. osmanthi. Pathogenicity test was performed using leaves of healthy F. tataricum plants. The conidial suspension (1 × 106 conidia/ml) collected from PDA cultures with 0.05% Tween 20 buffer was used for inoculation by spraying leaves of potted 20-day-old Tartary buckwheat cv. Pinku1. Five leaves of each plant were inoculated with spore suspensions (1 ml per leaf). An equal number of control leaves were sprayed with sterile water to serve as a control. The treated plants were kept in a greenhouse at 28°C and 80% relative humidity for 24 h, and then transferred to natural conditions with temperature ranging from 22 to 30°C and relative humidity ranging from 50 to 60%. Five days later, all N. osmanthi-inoculated leaves developed leaf spot symptoms similar to those observed in the field, whereas control leaves remained healthy. N. osmanthi was re-isolated from twelve infected leaves with frequency of 100%, fulfilling Koch's postulates. The genus Nigrospora has been regarded by many scholars as plant pathogens (Fukushima et al. 1998) and N. osmanthi is a known leaf blight pathogen for Stenotaphrum secundatum (Mei et al. 2019) and Ficus pandurata (Liu et al. 2019) but has not been reported on F. tataricum. Nigrospora sphaerica was also detected in vegetative buds of healthy Fagopyrum esculentum Moench (Jain et al. 2012). To our knowledge, this is the first report of N. osmanthi causing leaf spot on F. tataricum in China and worldwide. Appropriate strategies should be developed to manage this disease.
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http://dx.doi.org/10.1094/PDIS-08-20-1773-PDNDOI Listing
October 2020

[Application of micellar electrokinetic capillary chromatography for identification, quantitative detection and unfolding analysis of interleukin-12].

Nan Fang Yi Ke Da Xue Xue Bao 2020 Sep;40(9):1301-1306

Department of Pathophysiology, Wannan Medical College, Wuhu 241002, China.

Objective: To establish a micellar electrokinetic capillary chromatography-based method for identification and quantitative detection of interleukin-12 (IL-12) and analysis of its unfolding process.

Methods: An uncoated fused-silica capillary (inner diameter 50 μm) with a total length of 48.5 cm (40 cm to the detector) was used for the experiment. The factors influencing the separation efficiency of IL-12 were analyzed, and a standard curve of IL-12 concentration was established. The mixture of IL-12 and anti-IL-12 antibody was incubated in a water bath at 38 ℃ for 40 min, and capillary electrophoresis was then performed under the same conditions. The results were compared with those of IL-12 and anti-IL-12 antibody to identify IL-12. IL-12 and dithiothreitol (DTT) were incubated at 60 ℃ in water bath for different lengths of times, and the unfolding process of IL-12 was analyzed based on electrophoresis results of IL-12 in different states.

Results: A micellar capillary electrophoresis on-line sweep method was established with 80 mmol/L borate (pH=9.3) containing 30 mmol/L sodium dodecyl sulfate (SDS) as the buffer solution. This system showed a good linear relationship between the peak area and the mass concentration of IL-12 with a linear correlation coefficient of 0.9991 within the linear range of 2 to 120 ng/L. As the incubation time of IL-12 and DTT prolonged, the disulfide bond of IL-12 gradually opened and resulted in distinct changes in the protein peak.

Conclusions: This capillary electrophoresis-based method is simple and sensitive for IL-2 analysis and allows rapid detection of changes in IL-12 content in the setting of tumors and analysis of the possible causes.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2020.09.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544572PMC
September 2020

Understanding the roles of stress granule during chemotherapy for patients with malignant tumors.

Am J Cancer Res 2020 1;10(8):2226-2241. Epub 2020 Aug 1.

Department of Pathology, The Second Xiangya Hospital, Central South University Changsha 410011, Hunan, China.

The assembly of stress granules (SGs) is a conserved mechanism to regulate protein synthesis under cell stress, where the translation of global protein is silenced and selective protein synthesis for survival maintains. SG formation confers survival advantages and chemotherapeutic resistance to malignant cells. Targeting SG assembly may represent a potential treatment strategy to overcome the primary and acquired chemotherapeutic resistance and enhance curative effect. We conduct a comprehensive review of the published literatures focusing on the drugs that potentially induce SGs and the related mechanism, retrospect the relationship between SGs and drug resistance related proteins, illuminate the regulated pathways and potential targets for SG assembly, and discuss future directions of overcoming the resistance to chemotherapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471355PMC
August 2020

Both environmental and spatial variables affect bacterial functional diversity in mangrove sediments at an island scale.

Sci Total Environ 2021 Jan 31;753:142054. Epub 2020 Aug 31.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, People's Republic of China; School of Biology, Hainan Normal University, Haikou 571158, People's Republic of China. Electronic address:

Sediment microorganisms are influenced by various biotic and abiotic factors. However, information concerning the spatial factors that determine the functional diversity of sediment bacterial communities at an island scale is limited. Here, we conducted an island-scale study to assess the driving forces governing the functional diversity of sediment bacterial communities in different mangroves around the coast of Hainan Island, southern China. For mangrove sediments in Hainan Island, differences in the metabolic activity and functional diversity among four sites were context dependent, while that showed a trend of East > North > West > South. Furthermore, total carbon, nitrite nitrogen, and salinity are important environmental factors that determine the metabolic functional diversity of bacterial communities. This study also provided important insights for explaining the metabolic functional diversity of bacterial communities in tropical mangrove sediments. The metabolic activity had a significantly response to environmental variables (13.2% of pure variance was explained) and spatial variables (12.4%). More importantly, given that spatial variables may contribute to the bacterial functional as important as environmental variables, this spatial variety of bacterial functional provides new insight into studying bacterial functional biogeographic patterns and impacts on sediment-associated function.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142054DOI Listing
January 2021

miR-4634 augments the anti-tumor effects of RAD001 and associates well with clinical prognosis of non-small cell lung cancer.

Sci Rep 2020 08 4;10(1):13079. Epub 2020 Aug 4.

Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China.

MicroRNA (miRNA) is involved in the physiological and pathological processes of various malignancies. In this study, miRNA microarray analysis showed that miR-4634 levels in A549 cells increased significantly after everolimus (RAD001) treatment. Decreased expression of miR-4634 was also found in non-small-cell lung carcinoma (NSCLC) cell lines and patients' tumors by qPCR. Additionally, a combination of miR-4634 and RAD001 exerted synergistic antitumor efficacy by inhibiting cell proliferation, migration, and colony formation. High expression of miR-4634 was significantly more common in non-cancerous lung tissue than adenocarcinoma or squamous cell carcinoma tissue (72.8%, 45.7%, and 50.9%, respectively; P < 0.001). Furthermore, high expression of miR-4634 was found to be more frequent in patients without lymph node metastasis (P = 0.037) by in-situ hybridization. Importantly, through univariate and multivariate analysis, high miR-4634 expression was associated with better prognosis of NSCLC patients. In conclusion, miR-4634 may act as a tumor suppressor in NSCLC, and to augment the efficacy of RAD001, co-treatment of miR-4634 and RAD001 might be a potential mTOR-targeted cancer therapy strategy for NSCLC patients. High expression of miR-4634 could be an independent good prognostic biomarker for NSCLC.
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http://dx.doi.org/10.1038/s41598-020-70157-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403585PMC
August 2020

Comparative study of polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) in corals, sediments and seawater from coral reefs of Hainan, China.

Environ Pollut 2020 Sep 8;264:114719. Epub 2020 May 8.

Department of Environmental Resources and Management, Chia Nan University of Pharmacy and Science, Taiwan, China.

This work investigated levels of PAHs and HMs in fourteen species from seven genera of scleractinian corals, adjacent sediments, and surface seawater in Hainan, China. The sources of contaminations were analyzed as well. The results showed that scleractinian corals had a relatively higher bioaccumulation capacity for PAHs from sediments than for HMs. There were inter-species differences for these contaminants enriched in corals. Pavona varians and Porites lutea could accumulate PAHs more readily. While higher concentrations of Cr, Mn and Pb occurred in Favites flexuosa, other metal levels, such as for Ni, Cu, Zn and As, were found to be elevated in Pocillopora damicornis, as well as for Cd in Acropora echinata. It was found that PAHs originated from petrogenic and pyrolytic sources, and were mainly linked to onshore and on-sea activities, such as motorboats. Mn, Ni, As and Cd were from crustal materials or natural weathering, while Cr, Cu, Zn and Pb were non-crustal origin connecting with the use of anti-fouling boat paint and agricultural and/or aquacultural chemicals. This study suggested that corals could serve as good bioindicators for two types of chemical pollution in the reef system, especially for the two species P. varians and P. lutea for PAHs contaminants.
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http://dx.doi.org/10.1016/j.envpol.2020.114719DOI Listing
September 2020

Functional Genetic Variant in ATG5 Gene Promoter in Acute Myocardial Infarction.

Cardiol Res Pract 2020 21;2020:9898301. Epub 2020 Apr 21.

Shandong Provincial Key Laboratory of Cardiac Disease Diagnosis and Treatment, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, Shandong 272029, China.

Coronary artery disease (CAD) including acute myocardial infarction (AMI) is an inflammatory and metabolic disease mainly caused by atherosclerosis. Dysfunctional autophagy has been associated with abnormal lipid metabolism and inflammation. In previous studies, we have reported altered autophagic activity in AMI patients. As autophagy-related protein 5 (ATG5) is a core protein in autophagy, we speculated that altered ATG5 level may contribute to CAD and AMI development. In this study, the promoter of the ATG5 gene was genetically and functionally investigated in large groups of AMI patients ( = 378) and ethnic-matched healthy controls ( = 386). The results showed that a total of 15 genetic variants including 6 single-nucleotide polymorphisms (SNPs) in the ATG5 gene promoter were found in this study population. A novel deletion variant (g.106326168_70delTCT) and an SNP [g.106325757C > G (rs190825454)] were found in one 66-year-old male patient with non-ST-segment elevated AMI, but in none of controls. In cultured HEK-293 and H9c2 cells, the deletion variant significantly decreased the transcriptional activity of the ATG5 gene promoter ( < 0.01). In contrast, the genetic variants either identified only in controls or found in both AMI patients and controls did not affect the transcriptional activity of the ATG5 gene promoter ( > 0.05). Furthermore, an electrophoretic mobility shift assay showed that the deletion variant evidently affected the binding of a transcription factor. Therefore, the genetic variant identified in AMI may affect the activity of the ATG5 gene promoter and change the ATG5 level, contributing to AMI as a rare risk factor.
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http://dx.doi.org/10.1155/2020/9898301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191428PMC
April 2020

Highly Reversible and Rapid Sodium Storage in GeP with Synergistic Effect from Outside-In Optimization.

ACS Nano 2020 Apr 8;14(4):4352-4365. Epub 2020 Apr 8.

Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.

The composite GeP/C@rGO as a sodium ion battery anode material was fabricated by introducing a carbon matrix into GeP through high-energy ball milling, followed by encapsulating the resultant composite with graphene a solution-based ultrasonic method. To delineate the individual role of carbon matrix and graphene, material characterization and electrochemical analyses were performed for GeP/C@rGO and three other samples: bare GeP, GeP with graphene coating (GeP@rGO), and GeP with carbon matrix (GeP/C). GeP/C@rGO exhibits the highest electric conductivity (5.89 × 10 S cm) and the largest surface area (167.85 m g) among the four samples. The as-prepared GeP/C@rGO delivered a reversible high capacity of 1084 mA h g at 50 mA g, excellent rate capacity (435.4 mA h g at a high rate of 5 A g), and long-term cycling stability (400 cycles with a reversible capacity of 823.3 mA h g at 0.2 A g), all of which outperform the other three samples. The kinetics investigation reveals a "pseudocapacitive behavior" in GeP/C and GeP/C@rGO, where solely faradic reactions took place in bare GeP and GeP@rGO with a typical "battery behavior". Based on X-ray photoelectron spectroscopy and electrochemical impedance spectroscopy, the carbon matrix serves to activate and stabilize the interior of the composite, while the graphene protects and restrains the exterior surface. Benefiting from the synergistic combination of these two components, GeP/C@rGO achieved extremely stable cycling stability as well as outstanding rate performance.
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http://dx.doi.org/10.1021/acsnano.9b09869DOI Listing
April 2020

Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake.

Clin Transl Sci 2020 09 8;13(5):907-915. Epub 2020 Apr 8.

Department of Physiology, Anhui Medical College, Hefei, Anhui, China.

High-salt (HS) intake is closely associated with the ignition and progression of hypertension. The mechanisms might be involved in endothelial dysfunction, nitric oxide deficiency, oxidative stress, and proinflammatory cytokines. Propolis is widely used as a natural antioxidant and is a well-known functional food for its biological activities, which includes anti-inflammation, antimicrobial, and liver detoxification. In this study, we successfully replicated a HS diet-induced hypertensive rat model. We found that in the long-term HS diet group, the myocardial function of the rats was altered and led to a significant decrease (around 49%) in heart function. However, doses of Chinese water-soluble propolis (WSP) were found directly proportional (11%, 60%, 91%, respectively) to the myocardial function improvement in hypertensive rats. The results from the blood circulation test and hematoxylin-eosin stains showed that propolis had protective effects on myocardial functions and blood vessels in hypertensive rats. Also, based on the results of western blot and polymerase chain reaction, WSP effectively regulated Nox2 and Nox4 levels and was responsible for a decrease in reactive oxygen species synthesis. Our findings demonstrate that Chinese WSP has a significant effect on the blood pressure of hypertensive rats and their cardiovascular functions that improved significantly. The improvement in the cardiovascular functions might be related to the process of anti-oxidation, anti-inflammation, and the improvements of the endothelial function in hypertensive rats.
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http://dx.doi.org/10.1111/cts.12770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7938408PMC
September 2020

Histone demethylase KDM3B protects against ferroptosis by upregulating SLC7A11.

FEBS Open Bio 2020 04 18;10(4):637-643. Epub 2020 Mar 18.

Laboratory for Stem Cell and Regenerative Medicine, The Affiliated Hospital of Weifang Medical University, Weifang, China.

Ferroptosis is a type of adaptive cell death driven by cellular metabolism and iron-dependent lipid peroxidation. Though multiple genes (including SLC7A11 and GPX4) have been demonstrated to play key roles in ferroptosis, little is known about the epigenetic regulation of this process. Here, we report that KDM3B, a histone H3 lysine 9 demethylase, can protect against ferroptosis induced by Erastin, an inhibitor of SLC7A11. KDM3B overexpression in HT-1080 cells results in decreased histone H3 lysine 9 methylation. Furthermore, KDM3B upregulates the expression of SLC7A11 through cooperation with the transcription factor ATF4. In summary, we identify here KDM3B as a potential epigenetic regulator of ferroptosis.
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http://dx.doi.org/10.1002/2211-5463.12823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137800PMC
April 2020

Single tunnel-assisted endoscopic submucosal dissection for a 13-cm giant colorectal laterally spreading tumor.

Rev Esp Enferm Dig 2020 Feb;112(2):150-151

Gastroenterology, Xiangya No. 2 Hospital, China.

We report the case of a 60-year-old male with a giant colorectal laterally spreading tumor at the recto-sigmoid junction. A single tunnel-assisted endoscopic submucosal dissection was performed that constructed a submucosal tunnel along the longitude axis of the tumor. An en bloc resection was achieved with no perforation or post-operative bleeding and a comparatively low procedure time.
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http://dx.doi.org/10.17235/reed.2020.6297/2019DOI Listing
February 2020

Occurrence and potential health risks assessment of polycyclic aromatic hydrocarbons (PAHs) in different tissues of bivalves from Hainan Island, China.

Food Chem Toxicol 2020 Feb 31;136:111108. Epub 2019 Dec 31.

College of Ecology and Environment, Hainan University, Haikou, 570228, China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China; School of Biology, Hainan Normal University, Haikou, 571158, China. Electronic address:

The levels of 16 PAHs were determined in the adductor, gills, gonads, hepatopancreas and mantles of the pearl oyster (Pinctada martensii) and the mussel (Perna viridis) collected from coasts of Li'an and Xincun Bays. The levels of ΣPAHs ranged from 597.1 to 2332 ng g d w in the various tissues of bivalves. The pyrolytic source played an important role in the local coastal environment. Significantly higher levels of M-PAHs and H-PAHs were observed in Pinctada martensii than in Perna viridis. The ΣPAHs at different tissues showed the following order from high to low: mantles > hepatopancreas > gonads > gills > adductor. When levels of individual PAHs in the five bivalve tissues have been compared with each other, high correlations have been found (r = 0.793-0.975). A general trend was observed that log transformed BSAFs declined with increase of K values. The estimated amount of ΣPAHs via ingestion of oyster and mussel varied from 1.35 × 10-1.70 × 10 and 2.15 × 10-1.91 × 10 μg kg body weight day, respectively. The THQs and CRs calculated for regular consumption of raw bivalves were in the acceptable ranges and may not pose health risk concerns. But for certain population with higher consumption rate for PAHs contaminated bivalves, cautions should be taken for their higher cancer risk.
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http://dx.doi.org/10.1016/j.fct.2019.111108DOI Listing
February 2020

Optimization of Waterborne Poly(Urethane-Acrylate) Nanoemulsions Based on Cationic Polymerizable Macrosurfactants with Different Hydrophobic Side Chain Length.

Polymers (Basel) 2019 Nov 21;11(12). Epub 2019 Nov 21.

Shaanxi Key Laboratory of Chemical Additives for Industry, Shaanxi University of Science and Technology, Xi'an 710021, China.

In situ surfactant-free emulsion polymerization can help avoid the utilization of harmful co-solvents and surfactants in the preparation of waterborne poly(urethane-acrylate) (WPUA) nanoemulsion, but the solid content is extremely limited, which will affect the drying rate and film-forming properties. The utilization of polymerizable macrosurfactants can overcome the above problems. However, the research on cationic polymerizable macrosurfactants is extremely scarce. In this work, cationic dimethylaminoethyl methacrylate-b-alkyl methacrylates block copolymers (PDM--PRMA) with terminal double bonds and different hydrophobic side chain (HSC) lengths were fabricated via catalytic chain transfer polymerization (CCTP). HSC length of PDM--PRMA played an important role in the phase inversion, morphology, rheological behavior of WPUA nanoemulsions, as well as the comprehensive performance of WPUA/PDM--PRMA films. Polymerizable PDM--PBMA macrosurfactant had smaller molecular weight, lower surface tension and colloidal size than the random copolymer (PDM--PBMA) by traditional free radical polymerization. It was easy for PDM--PRMA to orientedly assemble at the oil/water interface and provide better emulsifying ability when the carbon number of HSC was four. Compared with WPUA/PDM--PBMA, WPUA/PDM--PBMA had a smaller particle size, stability and better film-forming properties. This work elucidated the mechanisms of HSC length in the fabrication of cationic PDM--PRMA and provides a novel strategy to prepare cationic WPUA of high performance.
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http://dx.doi.org/10.3390/polym11121922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960594PMC
November 2019

Genetic variants of VEGFR-1 gene promoter in acute myocardial infarction.

Hum Genomics 2019 11 19;13(1):56. Epub 2019 Nov 19.

Shandong Provincial Key Laboratory of Cardiac Disease Diagnosis and Treatment, Affiliated Hospital of Jining Medical University, Jining Medical University, 89 Guhuai Road, Jining, 272029, Shandong, China.

Background: Coronary artery disease (CAD) including acute myocardial infarction (AMI) is a common complex disease caused by atherosclerosis. Vascular epithelial growth factor receptor-1 (VEGFR-1) stimulates angiogenesis and vascular permeability, and functions as a decoy to sequester VEGF and prevent initiation of intracellular signaling. VEGFR-1 knockout mice exhibit significantly higher mortality due to heart failure, cardiac hypertrophy, and cardiac dysfunction. An evident increase in macrophage infiltration and cardiac fibrosis are also observed after transverse aortic constriction. Therefore, VEGFR-1 gene variants may be involved in CAD. In this study, VEGFR-1 gene promoter was genetically and functionally analyzed in large cohorts of AMI patients and ethnic-matched controls.

Results: A total of 16 DNA sequence variants (DSVs) including six single-nucleotide polymorphisms (SNPs) were found in the VEGFR-1 gene promoter and 5'-untranslated region. Five novel DSVs and one SNP were only identified in AMI patients group. These DSVs and SNP significantly altered the transcriptional activity of the VEGFR-1 gene promoter in both HEK-293 and H9c2 cells (P < 0.05). Further electrophoretic mobility shift assay indicated that the DSVs and SNPs evidently affected the binding of transcription factors.

Conclusions: The genetic variants in VEGFR-1 gene identified in AMI patients may alter the transcriptional activity of the VEGFR-1 gene promoter and change VEGFR-1 level, contributing to AMI development.
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http://dx.doi.org/10.1186/s40246-019-0243-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862733PMC
November 2019

Ultrasensitive photoelectrochemical microRNA biosensor based on doxorubicin sensitized graphitic carbon nitride assisted by a target-activated enzyme-free DNA walker.

Chem Commun (Camb) 2019 Oct;55(87):13082-13084

Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.

Herein, a photoelectrochemical biosensor was successfully constructed on the basis of a sensitization strategy of doxorubicin sensitized graphitic carbon nitride for the ultrasensitive detection of microRNA-141 with the assistance of a target-activated enzyme-free DNA walker.
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http://dx.doi.org/10.1039/c9cc06556cDOI Listing
October 2019

A photoelectrochemical biosensor based on fullerene with methylene blue as a sensitizer for ultrasensitive DNA detection.

Biosens Bioelectron 2019 Oct 9;142:111579. Epub 2019 Aug 9.

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address:

In this protocol, a photoelectrochemical (PEC) biosensor based on methylene blue (MB) sensitized fullerene (C) was designed for ultrasensitive DNA detection. First, C nanoparticles (C NPs) as photoactive material was modified on the electrode surface to obtain an initial photocurrent signal. Additionally, the nicking endonuclease (Nb.BvCI enzyme)-assisted signal amplification strategy was implemented to transform limited target to plenty of products, which hybridized with DNA2 to form plentiful DNA duplex for immobilizing numerous MB as the sensitizer, thus accomplishing the effective sensitization toward C NPs and achieving an enhanced photocurrent for quantitatively detecting target DNA. This established PEC strategy realized the target DNA detection with the detection limit down to 3.3 fM, which paved a new avenue for DNA determination and exhibited huge application potential for other biomarkers in clinical analysis and disease diagnosis.
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http://dx.doi.org/10.1016/j.bios.2019.111579DOI Listing
October 2019

Dihydromyricetin induced lncRNA MALAT1TFEB-dependent autophagic cell death in cutaneous squamous cell carcinoma.

J Cancer 2019 10;10(18):4245-4255. Epub 2019 Jul 10.

Surgery Department of Galactophore, The Affiliated Zhuzhou Hospital of Xiangya Medical College CSU, Zhuzhou 412000, China.

Cutaneous squamous cell carcinoma (CSCC) is the second most common skin cancer. Dihydromyricetin (DHM), a Rattan tea extract, has been shown to have antitumor activity with no obvious toxicity to normal cells in vitro and in vivo. However, its efficacy in the treatment of CSCC and the underlying antitumor mechanism has not been fully elucidated yet. In our study, DHM increased autophagic flux in the A431 cells, as evidenced by the upregulation of LC3-II and downregulation of P62/SQSTM1. Moreover, the pharmacological or genetic blocking autophagy decreased DHM-induced cell death, indicating DHM triggered autophagic cell death in A431 cells. Specifically, DHM induced TFEB de-phosphorylation, activated TFEB nuclear translocation and increased of TFEB reporter activity, which contributed to the expression of autophagy-related genes and subsequent initiated autophagic cell death in A431 cells. Importantly, DHM decreased lncRNA MALAT1 expression and MALAT1 overexpression abrogated the effects of DHM on TFEB-dependent autophagy both in vitro and in vivo. Taken together, DHM induces CSCC cell death via inducing excessive autophagy, which is mediated through the -TFEB pathway. Therefore, DHM may be beneficial for the development of chemotherapy for CSCC.
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http://dx.doi.org/10.7150/jca.32807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691703PMC
July 2019

A rare heterotopic gastric mucosa appearing between the muscularis mucosae and submucosa.

Rev Esp Enferm Dig 2019 Sep;111(9):712-713

Department of Gastroenterology, the Second Xiangya Hospital, Central South University, China.

To the best of our knowledge, this is the first report of ectopic gastric mucosa appearing between muscularis mucosae and submucosa, at esophagogastric junction. Currently there are two theories explaining the mechanism. The most widely accepted one is that Ectopic gastric mucosa is an embryological remnant, and an alternative theory is abnormal proliferation under inflammation1.Although we don't have many evidences to clarify this case into specific mechanism mentioned above, Nomura et reported two cases of Adenocarcinoma of the cervical esophagus arising from ectopic gastric mucosa, Completely resected by ESD with no signs of recurrence2. Therefore, In this present case, the lesion was successfully managed with ESD. The symptoms were released after the operation and the patient remained well during a year follow-up. Long-term follow-up is recommended in case of recurrence or gastric cancer.
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http://dx.doi.org/10.17235/reed.2019.6134/2018DOI Listing
September 2019

A pine wood sample preparation method for high target and quality DNA extraction for detection of Esteya vermicola by PCR from living pine.

J Basic Microbiol 2019 Apr 9;59(4):437-441. Epub 2019 Jan 9.

Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, South Korea.

Esteya vermicola is an endophytic fungus of pine wood nematode with demonstrated biocontrol efficacy. At present, the methods for detection of this fungus from pine wood is still inconvenient and inefficient such as thin-section microscopic observations. In the present study, a simple protocol was developed for wood sample preparation for effective extraction of fungal DNA from wood samples for PCR detection. The protocol of preparation of the sample involves washing in sterile water overnight on a shaking table followed by filtration and centrifugation to obtain the extracted DNA. The result indicates that with this sample preparation protocol, any proper DNA extraction method can be effectively used for the rapid and reliable detection of E. vermicola from pine wood. This method can provide valuable support for follow-up studies with practical applications, such as investigation of the growth rate of E. vermicola and how long it remains viable inside a pine tree.
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http://dx.doi.org/10.1002/jobm.201800522DOI Listing
April 2019

Effects of earthworms and effective microorganisms on the composting of sewage sludge with cassava dregs in the tropics.

J Air Waste Manag Assoc 2019 06 20;69(6):710-716. Epub 2019 Mar 20.

a Institute of Tropical Agriculture and Forestry, Hainan University , Haikou , People's Republic of China.

The present study revealed the role of earthworm-effective microorganisms (EM) in converting sewage sludge and cassava dregs into a valuable product. Sewage sludge was toxic to earthworm, therefore it was mixed with cassava dregs in 80:20 proportions (dry weight). Treatments included mixed substrate inoculated versus not inoculated with EM and treated with or without earthworms. The pH, total organic carbon, total nitrogen, and C:N ratio decreased from the initial measurements in the range of 17.43-18.46%, 25.48-33.82%, 19.60-25.37%, and 6.68-14.05% respectively; but electrical conductivity and available phosphorus increased in the range of 113.47-158.16% and 42.42-57.58%, respectively. In addition, they interactively increased total phosphorus from 19.84-63.01% and potassium from 16.41-50.78%, and decreased the polycyclic aromatic hydrocarbons content of substrate from 21.17% to 32.14% with an increase in earthworms from 51.71 to 57.69, respectively. Earthworms and EM could be used together as an efficient method for co-composting sewage sludge plus cassava dregs in the tropics. This could be expected to result in stabilization of waste, increase in nutrients, and reduction of pollutant content. : The first reports of interaction of earthworms and effective microorganisms in the treatment of sewage sludge and cassava dregs in the tropics. Co-composting was an efficient technology for treating sewage sludge and cassava dregs at the same time, in the tropics. The survival rate of the earthworms both> 95%, the highest number of cocoons (640.33) and hatchlings (4694.33) both in EW+EM (Earthworms added and EM inoculated) treatment. Earthworms and EM (Only EM inoculated) interactively increased total phosphorus and potassium content, and decreased the PAH content of substrate with increase in earthworms.
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http://dx.doi.org/10.1080/10962247.2018.1552215DOI Listing
June 2019

Comparative study of polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) in corals, surrounding sediments and surface water at the Dazhou Island, China.

Chemosphere 2019 Mar 16;218:157-168. Epub 2018 Nov 16.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China; Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China. Electronic address:

This study investigated polycyclic aromatic hydrocarbons (PAHs) content in corals (Acropora sp.), surficial sediments, and surface seawater, and heavy metals (HMs) contents in corals and sediments from Dazhou Island, Hainan, China. Concentrations of PAHs in seawater and sediment seasonally ranged from 191.5 ng L to 587.7 ng L, and from 37.9 ng g to 233 ng g, while levels in corals were higher (185.2-545.0 ng g) compared to those found in sediments, demonstrating bioaccumulation of PAHs by corals. A similar seasonally variation of PAHs was observed in water/sediments and corals, and the proportions of low molecular weight PAHs (LPAHs) in seawater and corals were higher. Pyrolytic and petrogenic contaminations were identified to be the main sources of PAHs. Lower HMs concentrations were detected in corals (9.8-39.4 μg g) than in sediments (65.0-83.3 μg g), but HMs bioaccumulation still occurs in corals. Higher concentrations of HMs in sediment and corals were detected in March and December, especially Mn and Zn. Application of an enrichment factor showed that Cu in corals was delivered from non-crustal materials, and anthropogenic inputs were possibly the main sources. According to Biota Sediment Accumulation Factor, corals could strongly bioaccumulate LPAHs and Cd, and PAHs at a higher (p < 0.05) rate than HMs. There was a lack of correlation between the accumulation of PAHs and HMs in corals based on the cluster analysis. Dual hierarchical clustering analysis result revealed that feeding, instead of symbiosis, might be the main process responsible for the bioaccumulation of PAHs and HMs.
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http://dx.doi.org/10.1016/j.chemosphere.2018.11.063DOI Listing
March 2019

Effects of Continuous Phase and Crosslinking Agent on the Rheological Behaviors and Properties of Cationic Poly(urethane-acrylate) Emulsifier-Free Microemulsions.

J Nanosci Nanotechnol 2018 12;18(12):8419-8425

Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science & Technology, Xi'an 710021, China.

Cationic poly(urethane-acrylate) emulsifier-free microemulsions are prepared by seeded emulsion polymerization (SPUA) or in-situ emulsion polymerization (IPUA) with (a) or without (b) crosslinking agent hydroxyethyl acrylate (HEA). Furthermore, no surfactant is introduced into the reaction system. The effect of continuous phase and HEA on the rheological behavior and properties of microemulsions are studied. The results show that IPUA using vinylmonomers as the continuous phase and with HEA (IPUAMB-b) has the highest ζ value, lowest coagulation ratio, smallest particle size of 48 nm and apparent core-shell structures. Rheological research has concluded that the zero-shear viscosity of IPUAB-b and IPUAM-b emulsion is 6.03×102 and 7.89 Pa·s, respectively, and presents pseudoplastic behavior. However, IPUAMB-b and SPUA-b display Newtonian behavior with η0 of 0.136 Pa·s and 0.0823 Pa·s, respectively. PUA emulsions display similar rheological behavior when NMP is replaced by the mixture of MMA and BA.
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http://dx.doi.org/10.1166/jnn.2018.16423DOI Listing
December 2018