Publications by authors named "Haihua Liu"

40 Publications

Visualization of Plasmonic Couplings Using Ultrafast Electron Microscopy.

Nano Lett 2021 07 21;21(13):5842-5849. Epub 2021 Jun 21.

Center for Nanoscale Materials, Argonne National Laboratory, Lemont, Illinois 60439, United States.

Hybrids of graphene and metal plasmonic nanostructures are promising building blocks for applications in optoelectronics, surface-enhanced scattering, biosensing, and quantum information. An understanding of the coupling mechanism in these hybrid systems is of vital importance to its applications. Previous efforts in this field mainly focused on spectroscopic studies of strong coupling within the hybrids with no spatial resolution. Here we report direct imaging of the local plasmonic coupling between single Au nanocapsules and graphene step edges at the nanometer scale by photon-induced near-field electron microscopy in an ultrafast electron microscope for the first time. The proximity of a step in the graphene to the nanocapsule causes asymmetric surface charge density at the ends of the nanocapsules. Computational electromagnetic simulations confirm the experimental observations. The results reported here indicate that this hybrid system could be used to manipulate the localized electromagnetic field on the nanoscale, enabling promising future plasmonic devices.
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http://dx.doi.org/10.1021/acs.nanolett.1c01824DOI Listing
July 2021

Preeclampsia-Associated lncRNA Regulates the Proliferation, Invasion, and Migration of Placental Trophoblast Cells.

Mol Ther Nucleic Acids 2020 Dec 9;22:684-695. Epub 2020 Oct 9.

Center for Genetics and Developmental Systems Biology, Department of Obstetrics & Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Preeclampsia is believed to be caused by impaired placentation with insufficient trophoblast invasion, leading to impaired uterine spiral artery remodeling and angiogenesis. However, the underlying molecular mechanism remains unknown. We recently carried out transcriptome profiling of placental long noncoding RNAs (lncRNAs) and identified 383 differentially expressed lncRNAs in early-onset severe preeclampsia. Here, we are reporting our identification of lncRNA as a potential causal factor of preeclampsia and its downstream pathways that may be involved in placentation. We found that was upregulated in patients and positively correlated with clinical severity. We systematically searched for potential -binding transcription factors and their targets in databases and found that the targets were enriched with differentially expressed genes in the placentae of patients. We further demonstrated that the lncRNA inhibited the invasion and migration of trophoblast cells through restraining the transcription factor CENPB from binding to the promoter of TNF receptor-associated factor 1 (). Therefore, we have identified the dysregulated pathway "-CENPB-TRAF1" as a contributor to the pathogenesis of preeclampsia through prohibiting the proliferation, invasion, and migration of trophoblasts during placentation.
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http://dx.doi.org/10.1016/j.omtn.2020.09.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585871PMC
December 2020

Association of lncRNA SH3PXD2A-AS1 with preeclampsia and its function in invasion and migration of placental trophoblast cells.

Cell Death Dis 2020 07 27;11(7):583. Epub 2020 Jul 27.

Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

Accumulating evidence suggests that the pathogenesis of preeclampsia involves poor placentation caused by insufficient trophoblast invasion and impaired uterine spiral artery remodeling, yet the underlying molecular mechanism remains unclear. We carried out transcriptome profiling on placentae from preeclamptic patients and normal subjects, and identified about four hundred long non-coding RNAs differentially expressed in placentae of patients with early-onset severe preeclampsia. Here, we report our identification of lncRNA SH3PXD2A-AS1 as a potential causal factor for this disease and its downstream pathways involved in placentation. We found that expression level of SH3PXD2A-AS1 in the placentae is positively correlated with clinical severity of the patients. We demonstrated that SH3PXD2A-AS1 inhibited invasion and migration through recruiting CCCTC-binding factor (CTCF) to the promoters of SH3PXD2A and CCR7 to inhibit their transcription. Therefore, we conclude that the upregulation of lncRNA SH3PXD2A-AS1 may contribute to the pathogenesis of preeclampsia through prohibiting trophoblast invasion during placentation.
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http://dx.doi.org/10.1038/s41419-020-02796-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385659PMC
July 2020

Landscape of Dysregulated Placental RNA Editing Associated With Preeclampsia.

Hypertension 2020 06 20;75(6):1532-1541. Epub 2020 Apr 20.

From the Center for Genetics and Developmental Systems Biology, Department of Obstetrics and Gynecology, Nanfang Hospital (Xiaoxue Yang, J.Y., X.L., Q.C., S.J., H.L., Y.G., Z.R., Y.Y., M.Z., Xinping Yang), Southern Medical University, Guangzhou, Guangdong, China.

Dysregulated RNA editing is well documented in several diseases, such as cancer and neurodegenerative diseases. The extent to which RNA editing might be involved in diseases originated in the placenta remains unknown. Here, we have systematically profiled RNA editome on the placentae, 9 from patients with early-onset severe preeclampsia (EOSPE) and 32 from normal subjects, and a widespread RNA editing dysregulation in EOSPE has been identified. The mis-edited gene set is enriched with known preeclampsia-associated genes and differentially expressed genes in EOSPE. The RNA editing events at 2 microRNA binding sites in 3'-untranslated region of the mRNA were generated, which could inhibit the microRNA-induced mRNA downregulation of in placenta-derived cell line, consistent with the observation in the placentae of preeclampsia patients. These results demonstrate the association of dysregulated placental RNA editing with preeclampsia, and providing a resource for further study on the role of RNA editing in the pathogenesis of this disease.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.14756DOI Listing
June 2020

Ampelopsin alleviates sevoflurane-induced cognitive dysfunction by mediating NF-κB pathway in aged rats.

Genes Genomics 2020 04 4;42(4):361-369. Epub 2020 Jan 4.

Department of Anesthesiology, Gaoyou Hospital Affiliated Soochow University, Gaoyou People's Hospital, No. 116 Fuqian Street, Gaoyou City, Yangzhou City, 225600, Jiangsu Province, China.

Background: Cancer-induced bone pain (CIBP) is the pain caused by bone metastasis from malignant tumors, and the largest source of pain for cancer patients. miR-300 is an important miRNA in cancer. It has been shown that miR-300 regulates tumorigenesis of various tumors.

Purpose: This study aims to investigate the role of miR-300 in CIBP and its underlying molecular mechanisms in vitro and in vivo.

Methods: We constructed CIBP model in rats and investigated the mechanism through which miR-300 affects CIBP. We first examined expression level of miR-300 in CIBP rats and then tested the effect of its overexpression. Next, we identified the target of miR-300 using TargetScan analysis and double luciferase assay. Finally, we studied genetic interactions between miR-300 and its target and their roles in CIBP.

Results: We found that miR-300 was downregulated in CIBP rats. Overexpression of miR-300 significantly attenuated cancer-induced neuropathic pain (p < 0.01). Furthermore, TargetScan analysis and double luciferase assay show High Mobility Group Box 1 (HMGB1) is a target of miR-300. Notably, HMGB1 is overexpressed in CIBP rats, while up-regulation of miR-300 significantly suppresses expression of HMGB1 (p < 0.01). Moreover, knockdown of HMGB1 by siRNA significantly relieves cancer-induced neuropathic pain in rats (p < 0.01). On the other hand, HMGB1 overexpression partially blocked the effect of miR-300 on cancer-induced nerve pain.

Conclusion: miR-300 relieves cancer-induced neuropathic pain by inhibiting HMGB1 expression. These results may be beneficial for the treatment of CIBP in clinical practice.
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http://dx.doi.org/10.1007/s13258-019-00897-5DOI Listing
April 2020

Expression and short-term prognostic value of miR-126 and miR-182 in patients with acute stroke.

Exp Ther Med 2020 Jan 21;19(1):527-534. Epub 2019 Nov 21.

Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.

Expression and short-term prognostic value of miR-126 and miR-182 in patients with acute stroke were investigated. In total, 153 patients with acute stroke admitted to the Second Affiliated Hospital of Soochow University from February 2016 to February 2018 were enrolled into the observation group as group A [88 patients with acute cerebral infarction (AIS)] or group B [65 patients with cerebral hemorrhage (ICH)]. Furthermore, 69 healthy people receiving physical examinations in the hospital were enrolled into the control group. The relative expression of miR-126 and miR-182 in all subjects were measured and their correlation with the National Institute of Health stroke scale (NIHSS) and activities of daily living (ADL) scores was analyzed. After 3 months of follow-up, the correlation of miR-126 and miR-182 with the Modified Rankin Scale (MRS) score of patients was investigated. The receiver operating characteristic (ROC) curve was employed to explore the value of miR-126 and miR-182, alone or in combination, in predicting the prognosis of acute stroke patients. Subjects in the control group had markedly higher miR-126 expression and lower miR-182 expression than those in group A and group B in the observation group (P<0.05). Pearson's correlation analysis suggested a notable correlation of miR-126 and miR-182 with NIHSS and ADL scores. Patients with a mild condition or good prognosis had higher miR-126 expression and lower miR-182 expression than patients with a severe condition or poor prognosis (P<0.05). Both miR-126 and miR-182 predicted the prognosis of acute stroke, and the combination of miR-126 and miR-182 presented better accuracy. The expression levels of miR-126 and miR-182 are associated with the neurological function, self-care ability, and prognosis in patients with acute stroke is highly valuable for predicting the prognosis of patients.
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http://dx.doi.org/10.3892/etm.2019.8227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923740PMC
January 2020

miR-300 mitigates cancer-induced bone pain through targeting HMGB1 in rat models.

Genes Genomics 2020 03 23;42(3):309-316. Epub 2019 Dec 23.

Department of Anesthesiology, Gaoyou People's Hospital, Gaoyou Hospital Affiliated Soochow University, 225600, Yangzhou, Jiangsu, China.

Background: Cancer-induced bone pain (CIBP) is the pain caused by bone metastasis from malignant tumors, and the largest source of pain for cancer patients. miR-300 is an important miRNA in cancer. It has been shown that miR-300 regulates tumorigenesis of various tumors.

Objective: This study aims to investigate the role of miR-300 in CIBP and its underlying molecular mechanisms in vitro and in vivo.

Methods: We constructed CIBP model in rats and investigated the mechanism through which miR-300 affects CIBP. We first examined expression level of miR-300 in CIBP rats and then tested the effect of its overexpression. Next, we identified the target of miR-300 using TargetScan analysis and double luciferase assay. Finally, we studied genetic interactions between miR-300 and its target and their roles in CIBP.

Results: We found that miR-300 was downregulated in CIBP rats. Overexpression of miR-300 significantly attenuated cancer-induced neuropathic pain (p < 0.01). Furthermore, TargetScan analysis and double luciferase assay show High Mobility Group Box 1 (HMGB1) is a target of miR-300. Notably, HMGB1 is overexpressed in CIBP rats, while up-regulation of miR-300 significantly suppresses expression of HMGB1 (p < 0.01). Moreover, knockdown of HMGB1 by siRNA significantly relieves cancer-induced neuropathic pain in rats (p < 0.01). On the other hand, HMGB1 overexpression partially blocked the effect of miR-300 on cancer-induced nerve pain.

Conclusion: miR-300 relieves cancer-induced neuropathic pain by inhibiting HMGB1 expression. These results may be beneficial for the treatment of CIBP in clinical practice.
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http://dx.doi.org/10.1007/s13258-019-00904-9DOI Listing
March 2020

Learning Nonclassical Receptive Field Modulation for Contour Detection.

IEEE Trans Image Process 2019 Sep 16. Epub 2019 Sep 16.

This work develops a biologically inspired neural network for contour detection in natural images by combining the nonclassical receptive field modulation mechanism with a deep learning framework. The input image is first convolved with the local feature detectors to produce the classical receptive field responses, and then a corresponding modulatory kernel is constructed for each feature map to model the nonclassical receptive field modulation behaviors. The modulatory effects can activate a larger cortical area and thus allow cortical neurons to integrate a broader range of visual information to recognize complex cases. Additionally, to characterize spatial structures at various scales, a multiresolution technique is used to represent visual field information from fine to coarse. Different scale responses are combined to estimate the contour probability. Our method achieves state-of-the-art results among all biologically inspired contour detection models. This study provides a method for improving visual modeling of contour detection and inspires new ideas for integrating more brain cognitive mechanisms into deep neural networks.
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http://dx.doi.org/10.1109/TIP.2019.2940690DOI Listing
September 2019

Transcriptome analysis reveals novel insights into the continuous cropping induced response in Codonopsis tangshen, a medicinal herb.

Plant Physiol Biochem 2019 Aug 5;141:279-290. Epub 2019 Jun 5.

Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Wuhan City, Hubei, 430074, PR China. Electronic address:

Codonopsis tangshen Oliv. (C. tangshen Oliv.), a famous medicinal herb in China, is seriously affected by continuous cropping (C-cro). The physiological and biochemical results indicated that C-cro significantly affected the malonaldehyde (MDA) and chlorophyll content, as well as activities of catalase (CAT) and superoxide dismutase (SOD) when compared with the non-continuous cropping (NC-cro) group. Transcriptome profiling found 762 differentially expressed genes, including 430 up-regulated and 332 down-regulated genes by C-cro. In addition, pathway enrichment analysis revealed that genes related to 'Tyrosine degradation I', 'Glycogen synthesis' and 'Phenylalanine and tyrosine catabolism' were up-regulated, and genes associated with 'Signal transduction', 'Immune system', etc. were down-regulated by C-cro. The expression of target genes was further validated by Q-PCR. In this study, we demonstrated the effects of C-cro on C. tangshen at the transcriptome level, and found possible C-cro responsive candidate genes. These findings could be further beneficial for improving the continuous cropping tolerance.
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http://dx.doi.org/10.1016/j.plaphy.2019.06.001DOI Listing
August 2019

Response to: Comment on "Systematic Review and Meta-Analysis of Diagnostic Accuracy of miRNAs in Patients with Pancreatic Cancer".

Dis Markers 2019;2019:6287315. Epub 2019 Feb 5.

Department of Epidemiology and Health Statistics, College of Public Health, Qingdao University, Qingdao, Shandong 266021, China.

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http://dx.doi.org/10.1155/2019/6287315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379861PMC
April 2019

Reporting and Methodological Quality of Systematic Reviews and Meta-Analyses of Nursing Interventions in Patients With Alzheimer's Disease: General Implications of the Findings.

J Nurs Scholarsh 2019 05 25;51(3):308-316. Epub 2019 Feb 25.

MD Candidate, Department of Epidemiology and Health Statistics, College of Public Health, Qingdao University, Qingdao, Shandong, China.

Objective: The aim of this study was to evaluate the reporting characteristics as well as the methodological quality of systematic reviews (SRs) and meta-analyses (MAs) of nursing interventions in patients with Alzheimer's disease and determine potential factors for high quality.

Methods: Following the inclusion and exclusion criteria, we searched the databases PubMed, EMBASE, and The Cochrane Library from inception through October 1, 2017. Two reviewers independently selected articles and extracted data. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist and Assessment of Multiple Systematic Reviews (AMSTAR) checklist were adopted to evaluate reporting and methodological quality, respectively.

Results: A total of 64 eligible articles, published from 2001 to 2017, were included. The mean PRISMA and AMSTAR scores were 19.310 ± 4.167 and 6.390 ± 2.208, respectively. For the PRISMA checklist, the following characteristics had less than 50% compliance: protocol or registration, full electronic search strategy, summary measures, risk for bias across studies, and synthesis of results. For the AMSTAR checklist, the following characteristics had less than 50% compliance: a priori study design, comprehensive literature search, status of publication used as inclusion criteria, scientific quality, publication bias, and conflicts of interest stated. Logistic regression analyses indicated that systematic reviews including meta-analyses that followed PRISMA guidelines, had a protocol or registration, and had funding support were related to higher reporting quality; systematic reviews including meta-analyses that had a protocol or registration were related to higher methodological quality.

Conclusions: The reporting and methodological quality of systematic reviews and meta-analyses of nursing interventions in patients with Alzheimer's disease were suboptimal, with some areas needing further improvement. More endorsement by journals of the reporting guidelines for SRs and MAs may improve article quality and the dissemination of reliable evidence to nurses. We recommend that authors, readers, reviewers, and editors become better acquainted with and adhere more strictly to the PRISMA and AMSTAR checklists.
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http://dx.doi.org/10.1111/jnu.12462DOI Listing
May 2019

The effects of the PRISMA statement to improve the conduct and reporting of systematic reviews and meta-analyses of nursing interventions for patients with heart failure.

Int J Nurs Pract 2019 Jun 20;25(3):e12729. Epub 2019 Feb 20.

Department of Epidemiology and Health Statistics, School of Public Health, Qingdao University, Qingdao, China.

Aims: The study aims to evaluate the reporting and methodological quality of systematic reviews and meta-analyses on nursing interventions in the field of heart failure and investigate whether reporting and methodological quality has been improved after PRISMA statement was published.

Methods: Pubmed, Cochrane Database of Systematic Review, and Embase databases were searched from inception of databases to July 31, 2018. Two authors independently extracted data from October 1, 2018, to October 31, 2018. AMSTAR and PRISMA checklists were used to assessed methodological and reporting quality.

Results: The 50 English articles satisfied inclusion criteria and were published from 2001 to 2017. After introduction of PRISMA statement, significant improvement in reporting of the following items was found: title, search, risk of bias in individual studies, summary measures, study selection, synthesis of results, summary of evidence for PRISMA checklists, and scientific quality of included studies provided (item 7) for AMSTAR checklists.

Conclusion: There were higher methodological and reporting quality after publication of PRISMA. We recommend authors, readers, reviewers, and editors to become more acquainted with and to more strictly adhere to the PRISMA and AMSTAR.
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http://dx.doi.org/10.1111/ijn.12729DOI Listing
June 2019

Exploring reporting quality of systematic reviews and Meta-analyses on nursing interventions in patients with Alzheimer's disease before and after PRISMA introduction.

BMC Med Res Methodol 2018 11 29;18(1):154. Epub 2018 Nov 29.

Department of Epidemiology and Health Statistics, School of Public Health, Qingdao University, No. 38, Dengzhou Road, Qingdao, 266021, Shandong, China.

Background: Systematic reviews (SRs) and meta-analyses (MAs) are distillation of current best available evidence, but are potentially prone to bias. The bias of SRs and MAs comes from sampling bias, selection bias and within study bias. So, their reporting quality is especially important as it may directly influence their utility for clinicians, nurses, patients and policy makers. The SRs and MAs on nursing interventions in patients with Alzheimer's disease (AD) have been increasingly published over the past decade, but the reporting quality of article has not been evaluated after the introduction of Preferred Reporting Items for Systematic Reviews and Meta Analyses (PRISMA) Statement.

Methods: According to the inclusion and exclusion criteria, we searched the databases including PubMed, EMBASE and The Cochrane Library from inception through October 16th 2018. Two reviewers independently selected articles and extracted data. The PRISMA checklist was adopted to evaluate reporting quality. Comparisons were made between studies published before (2001-2009) and after (2011-2018) its introduction.

Results: A total of 77 eligible articles, 18 (23.4%) were published before the PRISMA Statement and 59 (76.6%) were published afterwards. There was higher score after publication of the PRISMA Statement than before (20.83 ± 3.78 vs 17.11 ± 4.56, P <  0.05). There was an improvement in the following items after the PRISMA statement was released (P <  0.05): title (item 1, 50.0% vs 74.6%, OR = 3.10, 95CI%: 1.00-9.61), search (item8, 27.8% vs 57.6%,OR = 3.25, 95CI%: 1.14-9.28), study selection (item 9, 44.4% vs 81.4%,OR = 6.28, 95CI%: 1.93-20.37), Data collection process (item 10, 50.0% vs 76.3%,OR = 3.45, 95CI%:1.10-10.84), risk of bias in individual studies (item 12, 50.0% vs 83.1%, OR = 5.78, 95CI%:1.71-19.52), risk of bias across studies (item15, 5.6% vs 28.8%,OR = 3.60, 95CI%:1.04-12.43), study characteristics (item 18, 77.8% vs 98.3%, OR = 28.13, 95CI%:3.35-236.19), risk of bias with studies (item 19, 50.0% vs 83.1%, OR = 5.78, 95CI%:1.71-19.52), results in individual studies (item 20, 72.2% vs 94.9%, OR = 11.09, 95CI%:1.99-61.82), conclusions (item 26, 77.8% vs 98.3%, OR = 28.13, 95CI%:3.35-236.19). After controlling for the confounding factors, there were higher PRISMA score for systematic reviews including meta-analyses, protocol or registration, can't answer of RCT, journal source of SCI (Science Citation Index), manuscript length > 13 page and funding support.

Conclusion: Since the publication of the PRISMA Statement, there has been an improvement in the quality of reporting of SRs and MAs on nursing interventions in patients with AD. More endorsement by journals of the report guideline for SRs/MAs may improve articles reporting quality, and the dissemination of reliable evidence to nurses. We recommend authors, readers, reviewers, and editors to become more acquainted with and to more strictly adhere to the PRISMA checklist.
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http://dx.doi.org/10.1186/s12874-018-0622-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267794PMC
November 2018

Surface-Functionalized Silicon Nanoparticles as Anode Material for Lithium-Ion Battery.

ACS Appl Mater Interfaces 2018 Dec 11;10(51):44924-44931. Epub 2018 Dec 11.

Department of Chemistry , University of Tennessee , Knoxville , Tennessee 37996 , United States.

An epoxy group was successfully attached to the surface of silicon nanoparticle (SiNPs) via a silanization reaction between silanol-enriched SiNPs and functional silanes. The epoxy-functionalized SiNPs showed a much improved cell performance compared with the pristine SiNPs because of the increased stability with electrolyte and the formation of a covalent bond between the epoxy group and the polyacrylic acid binder. Furthermore, the anode laminate made from epoxy-SiNPs showed much enhanced adhesion strength. Post-test analysis shed light on how the epoxy-functional group affects the physical and electrochemical properties of the SiNP anode.
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http://dx.doi.org/10.1021/acsami.8b17729DOI Listing
December 2018

NF-kB mediated CX3CL1 activation in the dorsal root ganglion contributes to the maintenance of neuropathic pain induced in adult male Sprague Dawley rats1.

Acta Cir Bras 2018 Jul;33(7):619-628

MD, Physician, Department of Radiology, Gaoyou People's Hospital, Gaoyou Hospital Affiliated, Soochow University, China. Analysis and interpretation of data, critical revision.

Purpose: To evaluate the role of CX3CL1 and NF-κB in the lumbar disc herniation induced neuropathic pain.

Methods: After LDH induced by implantation of autologous nucleus pulposus (NP) on the left L5 nerve root was established, mechanical thresholds and thermal hyperalgesia were tested at relevant time points during an observation period of 28 days. Expression of CX3CL1 and NF-κBin the dorsal root ganglion (DRG) were performed by using Western blotting and RT-PCR.

Results: Implantation of autologous nucleus pulposus (NP) induced neuropathic pain, associated with increased mRNA and protein expression of CX3CL1 in the DRG. Moreover, intrathecal injection of neutralizing antibody against CX3CL1 could attenuates LDH-induced persistent pain hypersensitivity. Interestingly, NF-κB activation in the DRGs were found in LDH-induced neuropathic pain. Furthermore, NF-κB downregulation by p65 inhibitor PDTC markedly alleviated LDH-induced mechanical allodynia and thermal hyperalgesia in rat. Importantly, CX3CL1 neutralizing antibody (10 μg/10 μl, i.t.) reduces p-p65 protein level in DRG.

Conclusions: CX3XL1 could regulate LDH-induced neuropathic pain through NF-κB pathway. Targeting CX3CL1 and NF-κB may represent a potential treatment for neuropathic pain caused by LDH.
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http://dx.doi.org/10.1590/s0102-865020180070000007DOI Listing
July 2018

Systematic Review and Meta-Analysis of Diagnostic Accuracy of miRNAs in Patients with Pancreatic Cancer.

Dis Markers 2018 15;2018:6292396. Epub 2018 May 15.

Department of Epidemiology and Health Statistics, College of Public Health, Qingdao University, Qingdao, Shandong 266021, China.

Background: It is reported that miRNAs are aberrantly expressed in patients with pancreatic cancer. However, the diagnostic value of miRNAs in pancreatic cancer remains controversial. The meta-analysis was to access diagnostic accuracy of miRNAs in pancreatic cancer.

Methods: PubMed, Scopus, Web of Science, Chinese National Knowledge Infrastructure (CNKI), WANFANG Data, China Biomedical Literature Database (CBM), and VIP databases were retrieved up to June 30, 2016, to collect articles concerning the diagnosis of miRNAs in pancreatic cancer. The methodological quality of each study was assessed by the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2). This meta-analysis was conducted using RevMan5.0, MetaDiSc 1.4, and Stata 12.0 software.

Results: There are 40 articles including 109 studies. The pooled SEN was 0.81 (95% CI, 0.80-0.82), the pooled SPE was 0.78 (95% CI, 0.77-0.79), the pooled +LR was 3.32 (95% CI, 2.92-3.80), the pooled -LR was 0.27 (95% CI, 0.24-0.31), the pooled DOR was 14.56 (95% CI, 11.55-18.34), and pooled AUC was 0.86 (95% CI, 0.84-0.88).

Discussion: This meta-analysis demonstrated that miRNA makes a significant impact in the pancreatic cancer diagnosis with a high SEN and SPE, particularly using multiple miRNAs.
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http://dx.doi.org/10.1155/2018/6292396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977035PMC
October 2018

Reporting and methodological quality of survival analysis in articles published in Chinese oncology journals.

Medicine (Baltimore) 2017 Dec;96(50):e9204

Public Health College, Qingdao University, Qingdao, China.

Survival analysis methods have gained widespread use in the filed of oncology. For achievement of reliable results, the methodological process and report quality is crucial. This review provides the first examination of methodological characteristics and reporting quality of survival analysis in articles published in leading Chinese oncology journals.To examine methodological and reporting quality of survival analysis, to identify some common deficiencies, to desirable precautions in the analysis, and relate advice for authors, readers, and editors.A total of 242 survival analysis articles were included to be evaluated from 1492 articles published in 4 leading Chinese oncology journals in 2013. Articles were evaluated according to 16 established items for proper use and reporting of survival analysis.The application rates of Kaplan-Meier, life table, log-rank test, Breslow test, and Cox proportional hazards model (Cox model) were 91.74%, 3.72%, 78.51%, 0.41%, and 46.28%, respectively, no article used the parametric method for survival analysis. Multivariate Cox model was conducted in 112 articles (46.28%). Follow-up rates were mentioned in 155 articles (64.05%), of which 4 articles were under 80% and the lowest was 75.25%, 55 articles were100%. The report rates of all types of survival endpoint were lower than 10%. Eleven of 100 articles which reported a loss to follow-up had stated how to treat it in the analysis. One hundred thirty articles (53.72%) did not perform multivariate analysis. One hundred thirty-nine articles (57.44%) did not define the survival time. Violations and omissions of methodological guidelines included no mention of pertinent checks for proportional hazard assumption; no report of testing for interactions and collinearity between independent variables; no report of calculation method of sample size. Thirty-six articles (32.74%) reported the methods of independent variable selection. The above defects could make potentially inaccurate, misleading of the reported results, or difficult to interpret.There are gaps in the conduct and reporting of survival analysis in studies published in Chinese oncology journals, severe deficiencies were noted. More endorsement by journals of the report guideline for survival analysis may improve articles quality, and the dissemination of reliable evidence to oncology clinicians. We recommend authors, readers, reviewers, and editors to consider survival analysis more carefully and cooperate more closely with statisticians and epidemiologists.
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http://dx.doi.org/10.1097/MD.0000000000009204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5815752PMC
December 2017

A prognostic model for lung adenocarcinoma patient survival with a focus on four miRNAs.

Oncol Lett 2017 Sep 27;14(3):2991-2995. Epub 2017 Jun 27.

Department of Pharmacy, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.

There is currently no effective biomarker for determining the survival of patients with lung adenocarcinoma. The purpose of the present study was to construct a prognostic survival model using microRNA (miRNA) expression data from patients with lung adenocarcinoma. miRNA data were obtained from The Cancer Genome Atlas, and patients with lung adenocarcinoma were divided into either the training or validation set based on the random allocation principle. The prognostic model focusing on miRNA was constructed, and patients were divided into high-risk or low-risk groups as per the scores, to assess their survival time. The 5-year survival rate from the subgroups within the high- and low-risk groups was assessed. P-values of the prognostic model in the total population, the training set and validation set were 0.0017, 0.01986 and 0.02773, respectively, indicating that the survival time of the lung adenocarcinoma high-risk group was less than that of the low-risk group. Thus, the model had a good assessment effectiveness for the untreated group (P=0.00088) and the Caucasian patient group (P=0.00043). In addition, the model had the best prediction effect for the 5-year survival rate of the Caucasian patient group (AUC=0.629). In conclusion, the prognostic model developed in the present study can be used as an independent prognostic model for patients with lung adenocarcinoma.
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http://dx.doi.org/10.3892/ol.2017.6481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588086PMC
September 2017

Medical image classification via multiscale representation learning.

Artif Intell Med 2017 06 29;79:71-78. Epub 2017 Jun 29.

Huibei Key Laboratory for Medical Information Analysis and Tumor Treatment, Wuhan 430074, PR China. Electronic address:

Multiscale structure is an essential attribute of natural images. Similarly, there exist scaling phenomena in medical images, and therefore a wide range of observation scales would be useful for medical imaging measurements. The present work proposes a multiscale representation learning method via sparse autoencoder networks to capture the intrinsic scales in medical images for the classification task. We obtain the multiscale feature detectors by the sparse autoencoders with different receptive field sizes, and then generate the feature maps by the convolution operation. This strategy can better characterize various size structures in medical imaging than single-scale version. Subsequently, Fisher vector technique is used to encode the extracted features to implement a fixed-length image representation, which provides more abundant information of high-order statistics and enhances the descriptiveness and discriminative ability of feature representation. We carry out experiments on the IRMA-2009 medical collection and the mammographic patch dataset. The extensive experimental results demonstrate that the proposed method have superior performance.
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http://dx.doi.org/10.1016/j.artmed.2017.06.009DOI Listing
June 2017

Computational Model Based on Neural Network of Visual Cortex for Human Action Recognition.

IEEE Trans Neural Netw Learn Syst 2018 05 8;29(5):1427-1440. Epub 2017 Mar 8.

In this paper, we propose a bioinspired model for human action recognition through modeling neural mechanisms of information processing in two visual cortical areas: the primary visual cortex (V1) and the middle temporal cortex (MT) dedicated to motion. This model, named V1-MT, is composed of V1 and MT models (layers) corresponding to their cortical areas, which are built with layered spiking neural networks (SNNs). Some neuron properties in V1 and MT, such as direction and speed selectivity, spatiotemporal inseparability, and center surround suppression, are integrated into SNNs. Based on speed and direction selectivity, V1 and MT models contain multiple SNN channels, each of which processes motion information in sequences with spatiotemporal tunings of neurons at a certain speed and different directions. Therefore, we propose two operations, input signal perceiving with 3-D Gabor filters and surround inhibition processing with 3-D differences of Gaussian functions, to perform this task according to the spatiotemporal inseparability and center surround suppression of neurons. Then, neurons are modeled with our simplified integrate-and-fire model and motion information is transformed into spike trains. Afterward, we define a new feature vector: a mean motion map computed from spike trains in all channels to represent human actions. Finally, a support vector machine is trained to classify actions represented by the feature vectors. We conducted extensive experiments on public action databases, and the results show that our model outperforms other bioinspired models and rivals the state-of-the-art approaches.
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http://dx.doi.org/10.1109/TNNLS.2017.2669522DOI Listing
May 2018

LncRNA/DNA binding analysis reveals losses and gains and lineage specificity of genomic imprinting in mammals.

Bioinformatics 2017 May;33(10):1431-1436

Bioinformatics Section, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Motivation: Genomic imprinting is regulated by lncRNAs and is important for embryogenesis, physiology and behaviour in mammals. Aberrant imprinting causes diseases and disorders. Experimental studies have examined genomic imprinting primarily in humans and mice, thus leaving some fundamental issues poorly addressed. The cost of experimentally examining imprinted genes in many tissues in diverse species makes computational analysis of lncRNAs' DNA binding sites valuable.

Results: We performed lncRNA/DNA binding analysis in imprinting clusters from multiple mammalian clades and discovered the following: (i) lncRNAs and imprinting sites show significant losses and gains and distinct lineage-specificity; (ii) binding of lncRNAs to promoters of imprinted genes may occur widely throughout the genome; (iii) a considerable number of imprinting sites occur in only evolutionarily more derived species; and (iv) multiple lncRNAs may bind to the same imprinting sites, and some lncRNAs have multiple DNA binding motifs. These results suggest that the occurrence of abundant lncRNAs in mammalian genomes makes genomic imprinting a mechanism of adaptive evolution at the epigenome level.

Availability And Implementation: The data and program are available at the database LongMan at lncRNA.smu.edu.cn.

Contact: [email protected]

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btw818DOI Listing
May 2017

No Association between TNF-α -308G/A Polymorphism and Idiopathic Recurrent Miscarriage: A Systematic Review with Meta-Analysis and Trial Sequential Analysis.

PLoS One 2016 28;11(11):e0166892. Epub 2016 Nov 28.

Department of Clinical Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.

Background: Conflicting results were reported on the association between the TNF-α -308G/A polymorphism and idiopathic recurrent miscarriage (IRM). Though three meta-analyses have been conducted on this topic, the conclusions were contradictory, and the results may be unreliable as certain crucial conditions were neglected.

Method: A complete search was conducted in PubMed, Cochrane Library, and Embase, other sources like Google Scholar, ClinicalTrial.gov and reference lists of relevant articles were also retrieved. All candidate articles were accessed and screened using specific inclusion and exclusion criteria. Statistical analyses were performed on data extracted from eligible studies using the STATA 12.0 software and the TSA 0.9 beta software.

Results: Eventually, 12 case-control studies from 11 publications (with 1,807 cases and 2,012 controls) were included in this meta-analysis, and no evidence of any significant association was found in the overall analyses between the TNF-α -308G/A polymorphism and IRM risk. However, significant association was shown in Asian population (four studies from three publications) in the dominant model (AA + GA vs. GG), the allelic model (A vs. G), and the heterozygote model (GA vs. GG).

Conclusions: TNF-α -308G/A polymorphism is not associated with IRM risk. Though significant association was found in Asian population, the result needs further confirmation from more studies.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0166892PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125640PMC
June 2017

Diagnostic value of circulating miR-21: An update meta-analysis in various cancers and validation in endometrial cancer.

Oncotarget 2016 Oct;7(42):68894-68908

Medical Science Laboratory, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, China.

MiR-21 has been identified as one of the most common proto-oncogenes. It is hypothesized that up-regulated miR-21 could be served as a potential biomarker for human cancer diagnosis. However, inconsistencies or discrepancies about diagnostic accuracy of circulating miR-21 still remain. In this sense, miR-21's diagnostic value needs to be fully validated. In this study, we performed an update meta-analysis to estimate the diagnostic value of circulating miR-21 in various human cancers. Additionally, we conducted a validation test on 50 endometrial cancer patients, 50 benign lesion patients and 50 healthy controls. A systematical literature search for relevant articles was performed in Pubmed, Embase and Cochrane Library. A total of 48 studies from 39 articles, involving 3,568 cancer patients and 2,248 controls, were included in this meta-analysis. The overall sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the curve (AUC) were 0.76 (0.71-0.80), 0.82 (0.79-0.85), 4.3 (3.6-5.1), 0.29 (0.24-0.35), 15 (11-20) and 0.86 (0.83-0.89), respectively. In the validation test, the expression levels of serum miR-21 were significantly higher in benign lesion patients (p = 0.003) and endometrial cancer patients (p = 0.000) compared with that of healthy controls. Endometrial cancer patients showed higher miR-21 expression levels (p = 0.000) compared with benign lesion patients. In conclusion, the meta-analysis shows that circulating miR-21 has excellent performance on the diagnosis for various cancers and the validation test demonstrates that serum miR-21 could be served as a novel biomarker for endometrial carcinoma.
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http://dx.doi.org/10.18632/oncotarget.12028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356598PMC
October 2016

Infrared PINEM developed by diffraction in 4D UEM.

Proc Natl Acad Sci U S A 2016 Feb 4;113(8):2041-6. Epub 2016 Feb 4.

Physical Biology Center for Ultrafast Science and Technology, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125

The development of four-dimensional ultrafast electron microscopy (4D UEM) has enabled not only observations of the ultrafast dynamics of photon-matter interactions at the atomic scale with ultrafast resolution in image, diffraction, and energy space, but photon-electron interactions in the field of nanoplasmonics and nanophotonics also have been captured by the related technique of photon-induced near-field electron microscopy (PINEM) in image and energy space. Here we report a further extension in the ongoing development of PINEM using a focused, nanometer-scale, electron beam in diffraction space for measurements of infrared-light-induced PINEM. The energy resolution in diffraction mode is unprecedented, reaching 0.63 eV under the 200-keV electron beam illumination, and separated peaks of the PINEM electron-energy spectrum induced by infrared light of wavelength 1,038 nm (photon energy 1.2 eV) have been well resolved for the first time, to our knowledge. In a comparison with excitation by green (519-nm) pulses, similar first-order PINEM peak amplitudes were obtained for optical fluence differing by a factor of more than 60 at the interface of copper metal and vacuum. Under high fluence, the nonlinear regime of IR PINEM was observed, and its spatial dependence was studied. In combination with PINEM temporal gating and low-fluence infrared excitation, the PINEM diffraction method paves the way for studies of structural dynamics in reciprocal space and energy space with high temporal resolution.
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http://dx.doi.org/10.1073/pnas.1600317113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776498PMC
February 2016

Temporal Doppler Effect and Future Orientation: Adaptive Function and Moderating Conditions.

J Pers 2017 06 2;85(3):313-325. Epub 2016 Mar 2.

Department of Psychology and Beijing Key Laboratory of Behavior and Mental Health, Peking University.

The objectives of this study were to examine whether the temporal Doppler effect exists in different time intervals and whether certain individual and environmental factors act as moderators of the effect. Using hierarchical linear modeling, we examined the existence of the temporal Doppler effect and the moderating effect of future orientation among 139 university students (Study 1), and then the moderating conditions of the temporal Doppler effect using two independent samples of 143 and 147 university students (Studies 2 and 3). Results indicated that the temporal Doppler effect existed in all of our studies, and that future orientation moderated the temporal Doppler effect. Further, time interval perception mediated the relationship between future orientation and the motivation to cope at long time intervals. Finally, positive affect was found to enhance the temporal Doppler effect, whereas control deprivation did not influence the effect. The temporal Doppler effect is moderated by the personality trait of future orientation and by the situational variable of experimentally manipulated positive affect. We have identified personality and environmental processes that could enhance the temporal Doppler effect, which could be valuable in cases where attention to a future task is necessary.
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http://dx.doi.org/10.1111/jopy.12242DOI Listing
June 2017

[Aging law on anti-hypertensive effect of scrapping therapy on primary hypertension].

Zhongguo Zhen Jiu 2015 Jul;35(7):711-4

Objective: To observe the aging law on anti-hypertensive effect of scrapping therapy on primary hypertension so as to provide the reference evidence for the best treatment frequency of scrapping therapy.

Methods: Eighty-nine patients of primary hypertension complied with inclusive criteria were treated with scrapping therapy according to syndrome differentiation. The stimulated sites included the courses of the governor vessel and the bladder meridian on the neck and back region, the line from Quchi (LI 11) to Shousanli (LI 10) and that from Zusanli (ST 36) to Fenglong (ST 40). The strong stimulation for reducing attempt was applied to the case of excessi syndrome, determined by subcutaneous capillary rupture and subcutaneous blood stasis. The mild stimulation for reinforcing attempt was applied to the case of deficiency syndrome, determined by subcutaneous capillary hyperemia and skin flush. Each site was scrapped for 10 times, about 5 cm in width, for 15 min. One scrapping treatment was required. The blood pressure was taken as the observation index separately, named 2:00 pm, 2:30 pm, 3:00 pm, 3:30 pm, 4:00 pm, 5:00 pm, 6:00 pm and 8:00 pm one day before scrapping, the time before scrapping in the afternoon on the day of treatment, the moment (2:00 pm), 2:30 pm, 3:00 pm; 3:30 pm, 4:00 pm, 5:00 pm, 6:00 pm and 8:00 pm after scrapping, in 24 h and 48 h after scrapping.

Results: (1) After one scrapping, the blood pressure was reduced to be (137. 51±10. 24)/(81. 06±10. 56) mmHg half a hour after scrapping from (149. 00±10. 19)/(85. 30±10. 96) mmHg (1mmHg~0. 133 kPa) before scrapping (P<0. 01). (2) The differences were significant in blood pressure at each time point after scrapping as compared with that before scrapping (all P<0. 01). (3) Through nonlinear regression analysis, the systolic blood pressure after scrapping was increased up to 140 mmHg in 47. 2 h after scrapping.

Conclusion: Scrapping therapy achieves the apparent immediate anti-hypertensive effect on primary hypertension. It is suitable to apply scrapping treatment once every other day.
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July 2015

Observing in space and time the ephemeral nucleation of liquid-to-crystal phase transitions.

Nat Commun 2015 Oct 19;6:8639. Epub 2015 Oct 19.

Physical Biology Center for Ultrafast Science and Technology, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125, USA.

The phase transition of crystalline ordering is a general phenomenon, but its evolution in space and time requires microscopic probes for visualization. Here we report direct imaging of the transformation of amorphous titanium dioxide nanofilm, from the liquid state, passing through the nucleation step and finally to the ordered crystal phase. Single-pulse transient diffraction profiles at different times provide the structural transformation and the specific degree of crystallinity (η) in the evolution process. It is found that the temporal behaviour of η exhibits unique 'two-step' dynamics, with a robust 'plateau' that extends over a microsecond; the rate constants vary by two orders of magnitude. Such behaviour reflects the presence of intermediate structure(s) that are the precursor of the ordered crystal state. Theoretically, we extend the well-known Johnson-Mehl-Avrami-Kolmogorov equation, which describes the isothermal process with a stretched-exponential function, but here over the range of times covering the melt-to-crystal transformation.
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http://dx.doi.org/10.1038/ncomms9639DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4667692PMC
October 2015

Photon gating in four-dimensional ultrafast electron microscopy.

Proc Natl Acad Sci U S A 2015 Oct 5;112(42):12944-9. Epub 2015 Oct 5.

Physical Biology Center for Ultrafast Science and Technology, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, CA 91125

Ultrafast electron microscopy (UEM) is a pivotal tool for imaging of nanoscale structural dynamics with subparticle resolution on the time scale of atomic motion. Photon-induced near-field electron microscopy (PINEM), a key UEM technique, involves the detection of electrons that have gained energy from a femtosecond optical pulse via photon-electron coupling on nanostructures. PINEM has been applied in various fields of study, from materials science to biological imaging, exploiting the unique spatial, energy, and temporal characteristics of the PINEM electrons gained by interaction with a "single" light pulse. The further potential of photon-gated PINEM electrons in probing ultrafast dynamics of matter and the optical gating of electrons by invoking a "second" optical pulse has previously been proposed and examined theoretically in our group. Here, we experimentally demonstrate this photon-gating technique, and, through diffraction, visualize the phase transition dynamics in vanadium dioxide nanoparticles. With optical gating of PINEM electrons, imaging temporal resolution was improved by a factor of 3 or better, being limited only by the optical pulse widths. This work enables the combination of the high spatial resolution of electron microscopy and the ultrafast temporal response of the optical pulses, which provides a promising approach to attain the resolution of few femtoseconds and attoseconds in UEM.
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http://dx.doi.org/10.1073/pnas.1517942112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620897PMC
October 2015

Computational Model of Primary Visual Cortex Combining Visual Attention for Action Recognition.

PLoS One 2015 1;10(7):e0130569. Epub 2015 Jul 1.

School of Biomedical Engineering, South-Central University for Nationalities, Wuhan 430074, China; Key Laboratory of Cognitive Science of State Ethnic Affairs Commission, South-Central University for Nationalities, Wuhan 430074, China.

Humans can easily understand other people's actions through visual systems, while computers cannot. Therefore, a new bio-inspired computational model is proposed in this paper aiming for automatic action recognition. The model focuses on dynamic properties of neurons and neural networks in the primary visual cortex (V1), and simulates the procedure of information processing in V1, which consists of visual perception, visual attention and representation of human action. In our model, a family of the three-dimensional spatial-temporal correlative Gabor filters is used to model the dynamic properties of the classical receptive field of V1 simple cell tuned to different speeds and orientations in time for detection of spatiotemporal information from video sequences. Based on the inhibitory effect of stimuli outside the classical receptive field caused by lateral connections of spiking neuron networks in V1, we propose surround suppressive operator to further process spatiotemporal information. Visual attention model based on perceptual grouping is integrated into our model to filter and group different regions. Moreover, in order to represent the human action, we consider the characteristic of the neural code: mean motion map based on analysis of spike trains generated by spiking neurons. The experimental evaluation on some publicly available action datasets and comparison with the state-of-the-art approaches demonstrate the superior performance of the proposed model.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130569PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489578PMC
April 2016

Automatic segmentation of the left ventricle in cardiac MRI using local binary fitting model and dynamic programming techniques.

PLoS One 2014 11;9(12):e114760. Epub 2014 Dec 11.

Department of Radiology of Tongji Hospital affiliated to Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Segmentation of the left ventricle is very important to quantitatively analyze global and regional cardiac function from magnetic resonance. The aim of this study is to develop a novel algorithm for segmenting left ventricle on short-axis cardiac magnetic resonance images (MRI) to improve the performance of computer-aided diagnosis (CAD) systems. In this research, an automatic segmentation method for left ventricle is proposed on the basis of local binary fitting (LBF) model and dynamic programming techniques. The validation experiments are performed on a pool of data sets of 45 cases. For both endo- and epi-cardial contours of our results, percentage of good contours is about 93.5%, the average perpendicular distance are about 2 mm. The overlapping dice metric is about 0.91. The regression and determination coefficient between the experts and our proposed method on the LV mass is 1.038 and 0.9033, respectively; they are 1.076 and 0.9386 for ejection fraction (EF). The proposed segmentation method shows the better performance and has great potential in improving the accuracy of computer-aided diagnosis systems in cardiovascular diseases.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114760PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263664PMC
October 2015