Publications by authors named "Haihong Chen"

74 Publications

Enhanced recovery after surgery pathway reduces the length of hospital stay without additional complications in lumbar disc herniation treated by percutaneous endoscopic transforaminal discectomy.

J Orthop Surg Res 2021 Jul 17;16(1):461. Epub 2021 Jul 17.

Department of Orthopaedic Surgery, Minhang Hospital, Fudan University, 170 Xin Song Road, Shanghai, People's Republic of China.

Background: Enhanced recovery after surgery (ERAS) pathway in spine surgery is increasingly popular which can reduce the length of hospital stay (LOS). However, there are few studies on the safety and effectiveness of ERAS pathway in the treatment of single-level lumbar disc herniation (LDH) by percutaneous endoscopic transforaminal discectomy (PETD). The aim of this study was to investigate whether ERAS can reduce LOS of patients with single segment LDH treated by PETD.

Methods: We reviewed the outcomes of all LDH patients (L4/5) who had been treated with PETD at our institution. Quasi-experimental study was adopted between patients treated in an ERAS after PETD with those rehabilitated on a traditional pathway. The two groups were analyzed for LOS, operation time, complications, visual analog scale (VAS), Oswestry Dysfunction Index (ODI), hospitalization expenses (HE), and improved MacNab efficacy assessment criteria (MacNab).

Results: A total of 120 single segment LDH patients (ERAS pathway 60 cases, traditional care pathway 60 cases) who were selected from January 2019 to January 2021 met the inclusion criteria. There was a significant difference in mean LOS postoperative VAS scores and ODI on the 3rd day after surgery between the two groups (P < 0.05). The incidence of complications and HE were similar in the two groups (P > 0.05). The mean LOS decreased from 3.47 ± 1.14 days to 5.65 ± 1.39 days after application of ERAS pathway (P < 0.05).

Conclusions: The ERAS pathway reduced LOS without resulting in additional complications after PETD. These findings support the application of the perioperative ERAS pathway in the treatment of single-level LDH with PETD.

Level Of Evidence: Level IV, therapeutic.
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http://dx.doi.org/10.1186/s13018-021-02606-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285793PMC
July 2021

[Functional discovery and production technology for natural bioactive peptides].

Sheng Wu Gong Cheng Xue Bao 2021 Jun;37(6):2166-2180

Department of Chemical Engineering, Key Laboratory for Industrial Biocatalysis of the Ministry of Education, Tsinghua University, Beijing 100084, China.

Bioactive peptides play important roles in promoting human health, such as lowering blood pressure, blood sugar and blood lipid, anti-obesity, and anti-cancer. Thus, exploring functional bioactive peptides and developing efficient production technologies are of crucial importance. Herein, we review the development of function discovery and production technology for natural bioactive peptides. Presently, the top-down and bottom-up approaches are mainly used for the function discovery and production of natural active peptides. The top-down approach includes the direct extraction and identification for functional discovery, and the direct extraction, enzymatic hydrolysis and microbial fermentation for production. The bottom-up approach includes the polypeptide modification and database mining for functional discovery, and the chemical synthesis, enzyme synthesis, recombinant expression and cell-free synthesis for production. The top-down approach is usually associated with complicated process, lower efficiency, higher cost, harder quality control, and uncertain functionality, while the bottom-up approach is more suitable for the development of peptide drugs but difficult to be used for functional foods. With the technology development of sequencing and mass spectrometry, it is easier to obtain the proteomic information of various organisms at the molecular level. Based on the proteomic information, the top-down and bottom-up approaches can be combined to overcome the disadvantages of using these two approaches alone, thus providing a new strategy for the rapid development and production of natural active peptides.
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http://dx.doi.org/10.13345/j.cjb.210047DOI Listing
June 2021

Alteration of Histopathology in Recurrent Nasal Polyps.

ORL J Otorhinolaryngol Relat Spec 2021 Jul 1:1-7. Epub 2021 Jul 1.

Department of Otolaryngology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Objective: The objective of this study was to analyze the histopathologic changes in recurrent nasal polyps (NPs) in terms of tissue inflammatory cells infiltration and mucosal remodeling.

Methods: Thirty-five patients with primary NPs requiring a revision surgery during follow-up and a matched control group of 35 primary NP patients without recurrence were retrospectively enrolled. Histopathologic examination was performed of tissue inflammatory cells, subepithelial edema, epithelial cell hyperplasia, basement membrane thickness, and fibrosis.

Results: The mean eosinophil and neutrophil counts, proportions of eosinophil and neutrophil, as well as total inflammatory cell count were significantly higher in the recurrent group than those in the controls. Higher subepithelial edema was seen in the recurrent group. Within the recurrent group, the mean tissue eosinophil count and relative eosinophilia reduced from the first operation to revision surgery, whereas neutrophil count and proportion, and total inflammatory cell counts remained unchanged. Subepithelial edema and epithelial cell hyperplasia were significantly reduced after the first surgical procedure.

Conclusion: NP recurrence is associated with higher inflammatory grade (especially in eosinophilia and neutrophilia). Surgical and corticoid treatments may have an impact on mucosal histopathologic changes but need further certification.
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http://dx.doi.org/10.1159/000516430DOI Listing
July 2021

Inflammatory markers in postoperative cognitive dysfunction for patients undergoing total hip arthroplasty: a meta-analysis.

Aging Clin Exp Res 2021 Jun 27. Epub 2021 Jun 27.

Department of Pathology, The First Affiliated Hospital of Shantou University Medical College, No. 57 Changping Road, Chuangdong, Shantou, 515041, China.

Background: Postoperative cognitive dysfunction (POCD) is a poorly understood disorder, very common even after total hip arthroplasty (THA). It is widely considered that inflammation response play a role in the pathogenesis of POCD.

Aims: The aim of the present study was to investigate whether inflammation cytokine concentrations could serve as biomarkers for POCD in patients undergoing THA.

Methods: A systematic search of databases was conducted to retrieve publications measuring circulating inflammatory markers of patients with and without POCD after THA. Inflammatory markers identified in more than two studies were pooled. The standardized mean difference (SMD) and the 95% confidence interval (95% CI) were calculated for each outcome. Fail-safe N statistics was calculated to estimate possible publication bias.

Results: The pooled incidence rate of POCD after THA by combining 11 cohort studies was 31%. A total of five inflammatory markers, CRP, S-100B, IL-1β, IL-6 and TNF-α, were assessed. Significantly higher pre-operative CRP (P = 0.012) and S-100B (P < 0.0001) as well as post-operative CPR (P = 0.005) and IL-6 (P < 0.0001) at 6 h were found in POCD compared with non-POCD patients undergoing THA. Fail-safe N statistics revealed that these results are robust.

Discussion: The current evidence suggests that some of the inflammatory markers, including CRP, S-100B, and IL-6, were correlated with the occurrence of POCD after THA.

Conclusion: Monitor of inflammatory markers might help early diagnosis of POCD after THA and development of preventive strategies.
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http://dx.doi.org/10.1007/s40520-021-01919-7DOI Listing
June 2021

The Serum SIRT1 Protein is Associated with the Severity of Injury and Neurological Recovery in Mice with Traumatic Spinal Cord Injury.

Neuroscience 2021 08 22;469:103-109. Epub 2021 Jun 22.

Orthopedic Department, Minhang Center Hospital, Fudan University, Shanghai 201100, China. Electronic address:

The present study aimed to investigate the association between the serum SIRT1 protein and the severity of spinal cord injury (SCI) as well as the neurological recovery in mice. In this study, the wild-type (WT), Mx1-Cre SIRT1 (Mx1), and LCK-CreSIRT1 (LCK) mice were subjected to sham surgery, mild, moderate, or severe SCI, respectively. The serum was collected at intervals of 12 h, 1 day (d), 3 d, 5 d, 7 d, 10 d, 14 d, and 21 d after the injury. The locomotor function of all the animals was assessed using the Basso mouse scale (BMS) and the serum SIRT1 proteins were analyzed using enzyme-linked immunosorbent assay (ELISA). The results demonstrated that about 7-10 d after SCI, the levels of SIRT1 protein in the serum correlated significantly with the severity of the injury and at 28 d post-injury, there was a distant neurological recovery (BMS score). The serum SIRT1 concentration in both the Mx1 and LCK mice in the sham group was significantly reduced compared to that in the WT mice, and there was a delayed increase in the serum SIRT1 levels after injury. These findings indicate that the SIRT1 concentrations in the serum of the SCI mice closely correlated with the acute severity and neurological outcome.
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http://dx.doi.org/10.1016/j.neuroscience.2021.06.025DOI Listing
August 2021

Bioactive Dietary Fibers Selectively Promote Gut Microbiota to Exert Antidiabetic Effects.

J Agric Food Chem 2021 Jun 17;69(25):7000-7015. Epub 2021 Jun 17.

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang 330047, China.

High intake of dietary fibers was found to be inversely associated with type-2 diabetes (T2D), whereas the difference among different dietary fibers on T2D remains unclear. Therefore, we have investigated the effects of different dietary fibers on T2D. Nine types of dietary fibers were used to investigate and evaluate their effects on type-2 diabetic rats via physiology, genomics, and metabolomics. We found that supplementation with β-glucan, arabinogalactan, guar gum, apple pectin, glucomannan, and arabinoxylan significantly reduced the fasting blood glucose, whereas carrageenan, xylan, and xanthan gum did not affect glycemic control in diabetic rats. Also, bioactive dietary fibers (β-glucan, arabinogalactan, guar gum, and apple pectin) associated with the increased butyric acid level and abundance of beneficial bacteria (, , , , and some butyric acid-producing bacteria), as well as improved host metabolism by decreasing 12α-hydroxylated bile acids, acylcarnitines, and amino acids (leucine, phenylalanine, citrulline, etc.), thereby exert beneficial effects on T2D. It was also found that β-glucan might attenuate insulin resistance via downregulation of -mediated biosynthesis of branched-chain amino acids in T2D. Together, our study uncovered the effects of different dietary fibers on T2D, along with their potential mechanism.
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http://dx.doi.org/10.1021/acs.jafc.1c01465DOI Listing
June 2021

Bibliometric Insights in Genetic Factors of Substance-Related Disorders: Intellectual Developments, Turning Points, and Emerging Trends.

Front Psychiatry 2021 31;12:620489. Epub 2021 May 31.

Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Substance-related disorders are a group of medical conditions that affect a person's brain and behavior and lead to an inability to control the use of legal or illegal drug(s) or medication. Substance-related disorder is a serious public health and society problem worldwide. Genetic factors have been proven to have an important role. Researchers have carried out a lot of work in this field, and a large number of research results have been published in academic journals around the world. However, there are few overviews of research progress, presentation, and development trends in this field. In this study, a total of 636 articles related to genetic factors of substance-related disorders were retrieved from the Web of Science (WoS) database from 1997 to 2018, and the scientific literatures were analyzed by bibliometrics. The study found that the United States (US) has maintained a leading position in the field of research, with many core institutions and plenty of high-quality research results. Alcohol use disorder is still the most concerning issue in this field. Over the past 20 years, new techniques such as genome-wide association study (GWAS) based on high-throughput sequencing technology have replaced family studies, twin studies, and retrospective studies in this field. We believe that it is urgent to study the genetic factors of substance-related disorders, which can greatly deepen the understanding of the pathogenesis of substance-related disorders and may provide potential targets for precise treatment of such diseases.
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http://dx.doi.org/10.3389/fpsyt.2021.620489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200466PMC
May 2021

Dendrobium officinale polysaccharide triggers mitochondrial disorder to induce colon cancer cell death via ROS-AMPK-autophagy pathway.

Carbohydr Polym 2021 Jul 2;264:118018. Epub 2021 Apr 2.

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang, Jiangxi, 330047, China. Electronic address:

The homeostasis between mitochondrial function and autophagy is crucial to the physiological activity of cancer cells, and its mechanism is conducive to the development of anti-tumor drugs. Here, we aimed to explore the effect and mechanism of Dendrobium officinale polysaccharide (DOP) on colon cancer cell line CT26. Our data showed that DOP significantly inhibited the proliferation of CT26 cells and elevated autophagy level. Moreover, DOP disrupted mitochondrial function through increasing reactive oxygen species (ROS) and reducing mitochondrial membrane potential (MMP), thereby impairing ATP biosynthesis, which activated AMPK/mTOR autophagy signaling. Intriguingly, the further experiments demonstrated that DOP-induced cytotoxicity, excessive autophagy and mitochondrial dysfunction were reversed after CT26 cells pretreated with antioxidant (N-acetyl-l-cysteine). Herein, these findings implied that DOP-induced mitochondrial dysfunction and cytotoxic autophagy repressed the propagation of CT26 cells via ROS-ATP-AMPK signaling, providing a new opinion for the study of antineoplastic drugs.
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http://dx.doi.org/10.1016/j.carbpol.2021.118018DOI Listing
July 2021

Terahertz Spectroscopy for Accurate Identification of Basing on Nonconjugated 24(R)-Pseudoginsenoside F.

Plant Phenomics 2021 27;2021:6793457. Epub 2021 Jan 27.

Terahertz Technology Innovation Research Institute, Shanghai Key Lab of Modern Optical System, Terahertz Science Cooperative Innovation Center, University of Shanghai for Science and Technology, Shanghai Institute of Intelligent Science and Technology, Shanghai, China.

is a perennial herbaceous plant that contains many beneficial ginsenosides with diverse pharmacological effects. 24(R)-pseudoginsenoside F is specific to . , a useful biomarker for distinguishing this species from other related plants. However, because of its nonconjugated property and the complexity of existing detection methods, this biomarker cannot be used as the identification standard. We herein present a stable 24(R)-pseudoginsenoside F fingerprint spectrum in the terahertz band, thereby proving that F can be detected and quantitatively analyzed via terahertz spectroscopy. We also analyzed the sample by high-performance liquid chromatography-triple quadrupole mass spectrometry. The difference between the normalized data for the two analytical methods was less than 5%. Furthermore, . from different areas and other substances can be clearly distinguished based on these terahertz spectra with a standard principal component analysis. Our method is a fast, simple, and cost-effective approach for identifying and quantitatively analyzing . .
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http://dx.doi.org/10.34133/2021/6793457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043154PMC
January 2021

Clinical characteristics and outcome of SARS-CoV-2 infection during pregnancy.

J Infect 2021 06 6;82(6):e9-e10. Epub 2021 Apr 6.

Division of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Sun Yat-sen University, Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou 510080, Province Guangdong, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.jinf.2021.03.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021446PMC
June 2021

ZIP8 mediates the extracellular matrix degradation of nucleus pulposus cells via NF-κB signaling pathway.

Biochem Biophys Res Commun 2021 04 5;550:30-36. Epub 2021 Mar 5.

Department of Orthopaedic Surgery, Minhang Hospital, Fudan University, Shanghai, China. Electronic address:

The extracellular matrix (ECM) degradation of nucleus pulposus cells (NPCs) is mainly induced by metalloproteinases (MMPs). Zn is an essential component of MMPs, but the effect of Zn importers in controlling ECM metabolism remains unclear. The purpose of this research was to identify the involvement of Zn importers in ECM degradation induced by inflammatory stimuli and excessive mechanical stressing. In this study, NPCs from Sprague-Dawley (SD) rats were separated and cultured. FluoZin-3 AM staining was applied to detect [Zn]i in NPCs treated with Interleukin-1β (IL-1β) or cyclic tensile strain (CTS) with a Flexcell Strain Unit. We found that intracellular Zn concentration ([Zn]i) elevated dramatically, and ZIP8 is the predominant Zn importer among all importers in senescent NPCs. The [Zn]i and MMP expression level both increased in IL-1β and CTS treated NPCs. Furthermore, the expression of ZIP8 was also markedly increased. However, knockdown of ZIP8 with siRNA alleviated ECM degradation induced by inflammatory stimuli and CTS. Both stimuli activated NF-κB signaling pathway, and knockdown of ZIP8 effectively inhibited NF-κB signaling pathway activation. In conclusion, knockdown of ZIP8 can alleviate NPCs' ECM degradation caused by inflammatory stimuli and excessive mechanical stressing.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.129DOI Listing
April 2021

Multiomics Approach to Explore the Amelioration Mechanisms of Glucomannans on the Metabolic Disorder of Type 2 Diabetic Rats.

J Agric Food Chem 2021 Mar 19;69(8):2632-2645. Epub 2021 Feb 19.

State Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang, Jiangxi 330047, People's Republic of China.

Type 2 diabetes (T2D) is a worldwide epidemic associated with metabolic disorders and intestinal microbiota alterations. Polysaccharides have been considered to be beneficial to the prevention and alleviation of T2D. In the present study, ultra-performance liquid chromatography-triple-time-of-flight-based metabolomics and proteomics and 16S rRNA sequencing methods were employed to evaluate the effects of glucomannans from stem, konjac, and leaves on host metabolism and intestinal microbiota regulation in type 2 diabetic rats and potential mechanisms. The metabolism of amino acids was significantly disturbed in the type 2 diabetic rats, especially the upregulated branched-chain amino acid (BCAA) metabolism. Host-derived BCAA metabolism was significantly decreased in type 2 diabetic rats. However, the levels of BCAAs in host circulation and gene abundance of BCAA biosynthesis in gut microbiota were significantly increased in diabetic rats, which suggested that the disturbed intestinal microbiota might be responsible for the increased circulation of BCAAs in T2D. Glucomannan treatment decreased the abundance of microbial BCAA biosynthesis-related genes and ameliorated the host BCAA metabolism. Also, glucomannan with a higher molecular weight and a lower ratio of mannose/glucose possessed better antidiabetic effects. In summary, the antidiabetic effects of glucomannans might be associated with the amelioration of BCAA metabolism by modulating intestinal microbiota.
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http://dx.doi.org/10.1021/acs.jafc.0c07871DOI Listing
March 2021

Dynamic changes in lymphocyte subsets and parallel cytokine levels in patients with severe and critical COVID-19.

BMC Infect Dis 2021 Jan 18;21(1):79. Epub 2021 Jan 18.

Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, Institute of Pulmonary Diseases, Sun Yat-sen University, Guangzhou, 510080, Province Guangdong, People's Republic of China.

Background: The lack of knowledge regarding the pathogenesis and host immune response during SARS-CoV-2 infection has limited the development of effective treatments. Thus, we longitudinally investigated the dynamic changes in peripheral blood lymphocyte subsets and parallel changes in cytokine levels in COVID-19 patients with different disease severities to further address disease pathogenesis.

Methods: A total of 67 patients (10 moderate, 38 severe and 19 critical cases) with COVID-19 admitted to a tertiary care hospital in Wuhan from February 8th to April 6th, 2020 were retrospectively studied. Dynamic data of lymphocyte subsets and inflammatory cytokines were collected.

Results: On admission, compared with moderate cases, severe and critical cases showed significantly decreased levels of total lymphocytes, T lymphocytes, CD4 T cells, CD8 T cells, B cells and NK cells. IL-6 and IL-10 were significantly higher in the critical group. During the following hospitalization period, most of the lymphocyte subsets in the critical group began to recover to levels comparable to those in the severe group from the fourth week after illness onset, except for NK cells, which recovered after the sixth week. A sustained decrease in the lymphocyte subsets and an increase in IL-6 and IL-10 were observed in the nonsurvivors until death. There was a strong negative correlation between IL-6 and IL-10 and total lymphocytes, T lymphocytes, CD4 T cells, CD8 T cells and NK cells.

Conclusions: A sustained decrease in lymphocyte subsets, especially CD4 T cells and NK cells, interacting with proinflammatory cytokine storms was associated with severe disease and poor prognosis in COVID-19.
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http://dx.doi.org/10.1186/s12879-021-05792-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812569PMC
January 2021

A Bibliometric Insight of Genetic Factors in ASD: Emerging Trends and New Developments.

Brain Sci 2020 Dec 31;11(1). Epub 2020 Dec 31.

Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Autism spectrum disorder (ASD) cases have increased rapidly in recent decades, which is associated with various genetic abnormalities. To provide a better understanding of the genetic factors in ASD, we assessed the global scientific output of the related studies. A total of 2944 studies published between 1997 and 2018 were included by systematic retrieval from the Web of Science (WoS) database, whose scientific landscapes were drawn and the tendencies and research frontiers were explored through bibliometric methods. The United States has been acting as a leading explorer of the field worldwide in recent years. The rapid development of high-throughput technologies and bioinformatics transferred the research method from the traditional classic method to a big data-based pipeline. As a consequence, the focused research area and tendency were also changed, as the contribution of de novo mutations in ASD has been a research hotspot in the past several years and probably will remain one into the near future, which is consistent with the current opinions of the major etiology of ASD. Therefore, more attention and financial support should be paid to the deciphering of the de novo mutations in ASD. Meanwhile, the effective cooperation of multi-research centers and scientists in different fields should be advocated in the next step of scientific research undertaken.
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http://dx.doi.org/10.3390/brainsci11010033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824688PMC
December 2020

The complete mitochondrial genome of (Isoptera: Rhinotermitidae).

Mitochondrial DNA B Resour 2019 Nov 14;4(2):4063-4064. Epub 2019 Nov 14.

College of Agricultural and Food Science, Zhejiang A & F University, Hangzhou, P. R. China.

The mitochondrial genome of the contains 15,925 bp of nucleotide in length including 13 protein-coding genes, 22 transport RNAs, 2 ribosomal RNAs, and a non-coding region. The overall A + T among the genome sequence is 65.64%. The start codons of all protein-coding genes are ATN and the stop codon is TAA except TAG for Nad1 and incomplete T for COII and Nad5. The lengths of 12sRNA and 16sRNA genes are 743 and 1309 bp, respectively, and the control region was 1118 bp in length, with two repeating tandem regions. The phylogenetic tree revealed that and constituted a sister group to . The mitochondrial genome here provides a resource for evolution analysis within termites especially .
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http://dx.doi.org/10.1080/23802359.2019.1688701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707643PMC
November 2019

High Plasticity of the Gut Microbiome and Muscle Metabolome of Chinese Mitten Crab () in Diverse Environments.

J Microbiol Biotechnol 2021 Feb;31(2):240-249

Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture/National Demonstration Center for Experimental Fisheries Science Education/Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, P.R. China.

Phenotypic plasticity is a rapid response mechanism that enables organisms to acclimate and survive in changing environments. The Chinese mitten crab () survives and thrives in different and even introduced habitats, thereby indicating its high phenotypic plasticity. However, the underpinnings of the high plasticity of have not been comprehensively investigated. In this study, we conducted an integrated gut microbiome and muscle metabolome analysis on collected from three different environments, namely, an artificial pond, Yangcheng Lake, and Yangtze River, to uncover the mechanism of its high phenotypic plasticity. Our study presents three divergent gut microbiotas and muscle metabolic profiles that corresponded to the three environments. The composition and diversity of the core gut microbiota (Proteobacteria, Bacteroidetes, Tenericutes, and Firmicutes) varied among the different environments while the metabolites associated with amino acids, fatty acids, and terpene compounds displayed significantly different concentration levels. The results revealed that the gut microbiome community and muscle metabolome were significantly affected by the habitat environments. Our findings indicate the high phenotypic plasticity in terms of gut microbiome and muscle metabolome of when it faces environmental changes, which would also facilitate its acclimation and adaptation to diverse and even introduced environments.
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http://dx.doi.org/10.4014/jmb.2011.11018DOI Listing
February 2021

Comparative pharmacokinetics of intravenous and intramuscular cefquinome sulfate administration in ducklings and goslings.

Am J Vet Res 2020 Nov;81(11):837-877

Objective: To compare the pharmacokinetics of cefquinome sulfate in ducklings and goslings after IV or IM administration of a single dose.

Animals: 216 healthy Muscovy ducklings () and 216 healthy Sichuan white goslings ().

Procedures: Ducklings and goslings were each randomly assigned to 3 groups (n = 72/group) that received a single dose (2 mg/kg) of injectable cefquinome sulfate administered IV or IM or of injectable cefquinome sulfate suspension administered IM. Blood samples were collected at various points after drug administration (n = 6 birds/time point). Plasma cefquinome concentrations were measured by high-performance liquid chromatography with UV detection, and pharmacokinetic parameters were calculated with a 2-compartment model method.

Results: After IV injection, mean distribution half-life of cefquinome was longer in goslings (0.446 hours) than in ducklings (0.019 hours), whereas volume of distribution at steady state was greater (0.432 vs 0.042 L/kg) and elimination half-life was slower (1.737 vs 0.972 hours). After IM administration of injectable cefquinome sulfate, bioavailability of the drug was higher in goslings (113.9%) than in ducklings (67.5%). After IM administration of injectable cefquinome sulfate suspension, bioavailability was also higher in goslings (123.1%) than in ducklings (96.8%), whereas elimination half-life was slower (6.917 vs 1.895 hours, respectively).

Conclusions And Clinical Relevance: In goslings, IV administration of cefquinome resulted in slower distribution and metabolism of the drug than in ducklings and IM administration resulted in higher bioavailability. The delayed-release effect of the injectable cefquinome sulfate suspension when administered IM was observed only in goslings.
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http://dx.doi.org/10.2460/ajvr.81.11.873DOI Listing
November 2020

Dendrobium officinale polysaccharide ameliorates the liver metabolism disorders of type II diabetic rats.

Int J Biol Macromol 2020 Dec 5;164:1939-1948. Epub 2020 Aug 5.

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. Electronic address:

Type 2 diabetes (T2D) is a complicated endocrine metabolic disease, accompanied with oxidative stress injury and low-grade inflammation. The effects of polysaccharide extracted from Dendrobium officinale stem (DOP) on oxidative stress, inflammation and dysregulated metabolism in the liver of type 2 diabetic rats and its potential mechanism were evaluated in the study. Here, ultra performance liquid chromatography - quadrupole - time - of - flight (UPLC-Q-TOF) mass spectrometry-based lipidomics and metabolomics analysis were carried out to study the amelioration of DOP on the liver metabolism disorders of type 2 diabetic rats. Lipidomics analysis indicated that the disturbed degree of fatty acid, glycerolipid (diacylglycerol and triacylglycerol), and glycerophospholipid (phosphatidylcholine and phosphatidylethanolamine) metabolism were mitigated by the DOP treatment. Metabolomics analysis revealed that the DOP treatment balanced the metabolism of ceramide and bile acids, including deoxycholic acid, taurocholic acid, and cholic acid. In addition, the symptoms of oxidative stress, inflammation, and hepatic lipid accumulation of liver were ameliorated by DOP administration. The above results suggest that the study of functional ingredient application of DOP might be meaningful for the management of T2D.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.007DOI Listing
December 2020

Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level.

J Phycol 2020 10 19;56(5):1339-1348. Epub 2020 Jun 19.

Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao, 266003, China.

The determination of the ploidy level of an organism is a prerequisite for studies of evolution, cellular function, and genomic construction. Identification of the ploidy of the economically important red alga Gracilariopsis lemaneiformis has been hindered by its small genome and large number of chromosomes. Therefore, in the current study, PloidyNGS, a tool that calculates the number of reads supporting different alleles at each position along the genome sequence, and fluorescence in situ hybridization coupled with tyramide signal amplification (TSA-FISH) were used to clarify the ploidy of G. lemaneiformis. In addition, flow cytometry (FCM) was used to estimate the ploidy of different somatic cells. The PloidyNGS results showed that most of the alleles in the gametophyte were monomorphic, whereas the TSA-FISH results showed that one hybridization signal was observed in gametophytic nuclei and two in tetrasporophytic nuclei when the nuclei were hybridized by single copy gene probes. These results confirmed that G. lemaneiformis is a haploid in the gametophytic generation and diploid in the sporophytic generation. Moreover, the FCM result suggested that G. lemaneiformis was not an endopolyploid. Based on previous studies, we hypothesize that the nuclear number is important for the cellular differentiation and function of this species. We also suggest that G. lemaneiformis evolved from a paleopolyploid, the genome of which has been diploidized, and that traces of genomic doubling are no longer apparent. Thus, this study provides important evidence for further studies on the evolution and genomes of red algae.
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http://dx.doi.org/10.1111/jpy.13035DOI Listing
October 2020

Adherence to drug therapy for hypertensive disorders of pregnancy: a cross-sectional survey.

Arch Public Health 2020 8;78:41. Epub 2020 May 8.

1School of Medicine and Health Management, Tongji Medical College, Huazhong University of Science and Technology, No.13. Hangkong Road, Wuhan, 430030 Hubei Province China.

Background: Hypertensive disorders of pregnancy (HDPs) are a major contributor to maternal mortality worldwide, and drug therapy for HDPs is complicated and special. Clinical guidelines help physicians optimize the care for HDPs, but little is known about whether physicians adhere to drug therapy guidelins well, especially in China. This study aims to evaluate adherence to the drug therapy guidelines of the Chinese Obstetricians and Gynecologists Association (COGA) for HDPs and to explore the corresponding associations with recommendation evidence.

Methods: A cross-sectional design was executed for 306 women with HDPs hospitalized in a maternity ward of a tertiary hospital from August 2014 to July 2015 in Hubei, China. Adherence to the COGA guidelines was evaluated according to six items: the time of use and route of administration and dosage of antihypertensive drugs, MgSO, and corticosteroids. Binary logistic regression was adopted to explore the associations between adherence to clinical decisions and recommendation evidence.

Results: The average adherence rate for drug therapy for HDPs was 48.22%. The adherence rate for the time of antihypertensive drug and corticosteroid use scored 95.65 and 86.75%, whereas the other four items of the time of MgSO use and the routes of administration and dosages of antihypertensive drugs, MgSO, and corticosteroids scored < 50.00%. High- and low-evidence-based recommendations were followed in 40.00 and 54.70% of the decisions, respectively. Logistic regression revealed that recommendation evidence (OR = 0.588,  = 0.003) was associated with adherence.

Conclusions: Further improvement is still needed to achieve good adherence, especially regarding the time of MgSO use and drug dosage. High-evidence-based management of drug therapy for HDPs should be strengthened.
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http://dx.doi.org/10.1186/s13690-020-00423-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206801PMC
May 2020

What is the meaning of health literacy? A systematic review and qualitative synthesis.

Fam Med Community Health 2020 05;8(2)

School of Medicine and Health Management, Huazhong University of Science and Technology Tongji Medical College, Wuhan, China

The objective of this review was to clarify what health literacy represents. A systematic review with qualitative syntheses was performed (CRD42017065149). Studies concerning health literacy in all settings were included. Studies before 15 March 2017 were identified from PubMed, Medline, Embase, Web of Science, Scopus, PsycARTICLES and the Cochrane Library. The included literature either had defined the concept of health literacy or made a detailed explanation of health literacy. A total of 34 original studies met the inclusion criteria, including 13 involved in previous systematic reviews and 21 new studies. Health literacy was commonly conceptualised as a set of knowledge, a set of skills or a hierarchy of functions (functional-interactive-critical). The construct of health literacy covers three broad elements: (1) knowledge of health, healthcare and health systems; (2) processing and using information in various formats in relation to health and healthcare; and (3) ability to maintain health through self-management and working in partnerships with health providers. Health literacy is defined as the ability of an individual to obtain and translate knowledge and information in order to maintain and improve health in a way that is appropriate to the individual and system contexts. This definition highlights the diversity of needs from different individuals and the importance of interactions between individual consumers, healthcare providers and healthcare systems.
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http://dx.doi.org/10.1136/fmch-2020-000351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239702PMC
May 2020

Longshengzhi Capsules Improve Ischemic Stroke Outcomes and Reperfusion Injury via the Promotion of Anti-Inflammatory and Neuroprotective Effects in MCAO/R Rats.

Evid Based Complement Alternat Med 2020 9;2020:9654175. Epub 2020 Mar 9.

School of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.

Stroke is the leading cause of death in the elderly. Traditional Chinese medicine provides an exciting strategy for treating stroke. Previous reports indicated that Longshengzhi capsules (LSZ), a modified Chinese formula, reduced formed thrombi and oxidative stress and were promising in the clinical treatment of ischemic stroke. However, the specific therapeutic effect and mechanism of LSZ are still ambiguous. This study aimed to define the effects of LSZ on proinflammatory mediators and neuroprotective effects on middle cerebral artery occlusion and refusion (MCAO/R) rats. Rats were treated with different doses of LSZ (0.54, 1.62, and 4.32 g/(kg·d)) in a week after model building. LSZ could improve the survival rate, ischemic stroke outcome, and infarct volume. In addition, significant decrease was observed in reactive oxygen species (ROS) levels and inflammatory factor levels in LSZ-treated groups, concomitant with increase in activities of superoxide dismutase (SOD), neurosynaptic remodeling, and decrease in brain edema. It is proposed that LSZ has anti-inflammatory and neuroprotective effects resulting in downregulating matrix metalloproteinase 2/9 (MMP-2/9) and vascular endothelial growth factor (VEGF) and nuclear factor kappa-B (NF-B) and upregulating microtubule-associated protein-2 (Map-2) and growth-associated protein-43 (GAP-43) via p38 MAPK and HIF-1 signaling pathways in MCAO/R rats. This study provides potential evidences that p38 MAPK and HIF-1/VEGF signaling pathways play significant roles in the anti-inflammatory and neuroprotective effects of LSZ.
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http://dx.doi.org/10.1155/2020/9654175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085377PMC
March 2020

Withdrawn: Clinical manifestations and outcome of SARS-CoV-2 infection during pregnancy.

J Infect 2020 Mar 5. Epub 2020 Mar 5.

The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Province Guangdong, PR China. Electronic address:

This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.
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http://dx.doi.org/10.1016/j.jinf.2020.02.028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133645PMC
March 2020

Effects of Nondigestible Oligosaccharides on Obesity.

Annu Rev Food Sci Technol 2020 03 17;11:205-233. Epub 2020 Jan 17.

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China; email:

Obesity is a major public health concern that has almost reached the level of pandemic and is rapidly progressing. The gut microbiota has emerged as a crucial regulator involved in the etiology of obesity, and the manipulation of it by dietary intervention has been widely used for reducing the risk of obesity. Nondigestible oligosaccharides (NDOs) are attracting increasing interests as prebiotics, as the indigestible ingredients can induce compositional or metabolic improvement to the gut microbiota, thereby improving gut health and giving rise to the production of short-chain fatty acids (SCFAs) to elicit metabolic effects on obesity. In this review, the role NDOs play in obesity intervention via modification of the gut microecology, as well as the physicochemical and physiological properties and industrial manufacture of NDOs, is discussed. Our goal is to provide a critical assessment of and stimulate comprehensive research into NDO use in obesity.
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http://dx.doi.org/10.1146/annurev-food-032519-051743DOI Listing
March 2020

Prognostic Significance of Serum Complement Component 3 in Chronic Rhinosinusitis with Nasal Polyps.

ORL J Otorhinolaryngol Relat Spec 2020;82(2):67-73. Epub 2020 Jan 14.

Department of Otolaryngology, The First Affiliated Hospital, School of Medicine, ZheJiang University, Hangzhou, China.

Objective: The aim of this study was to assess the relationship between serum complement component 3 (C3) levels and disease recurrences in patients with chronic rhinosinusitis with nasal polyps (NPs).

Methods: Ninety-seven patients with NPs and 30 controls were recruited. Clinical features were collected. Serum concentrations of C3 and C4 were measured before and after endoscopic sinus surgery.

Results: Compared to the controls, increased C3 levels were found in patients with NPs. Patients with polyp recurrences had higher pre- and postoperative serum C3 levels than patients without polyp recurrences. Serum C3 levels dropped after surgery. After polyp regrowth, the mean C3 level in the recurrent group elevated again to the degree similar to that before surgery. When patients were stratified by tissue eosinophilia, no significant difference was seen in pre-/postoperative, absolute change after surgery, and post-recurrent C3 levels between patients without and with eosinophilic NPs in the group with disease recurrences.

Conclusion: Serum C3 may be involved in the pathogenesis of NPs. Higher serum C3 levels may pinpoint patients at high risk of recurrence as an independent factor. Furthermore, the change in C3 levels after surgery may have the potential to serve as a predictor for polyp progression. Adding serum C3 measurement to the routine walk-up in the clinical management of NPs is worth further investigation and may help physicians make a more rational diagnostic and/or therapeutic decision regarding this disease.
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http://dx.doi.org/10.1159/000504195DOI Listing
February 2021

Heterozygous Single Nucleotide Polymorphic Loci in Haploid Gametophytes of (Rhodophyta).

Front Genet 2019 6;10:1256. Epub 2019 Dec 6.

Key Laboratory of Marine Genetics and Breeding (Ocean Univeristy of China), Ministry of Education, Qingdao, China.

is an important commercial macroalga. Whole-genome resequencing was conducted to identify single nucleotide polymorphisms (SNPs) in parental gametophytes and 60 F gametophytes of in this study, and 9,989 SNPs located in nonrepetitive sequences were obtained. Among these SNPs, 92.02% of loci were identified as having a heterozygous genotype in at least one gametophyte, and 48.07% of loci had identical heterozygous genotypes in the 62 gametophytes. For each gametophyte, the proportions of homozygous and heterozygous loci ranged between 13.74 and 21.61% (mean of 17.04%) and between 66.36 and 83.59% (mean of 77.12%), respectively. The remainder were missing loci, representing an average 5.84%. Sources of heterozygous SNPs were free of exogenous DNA contamination, cross contamination among individuals, plastid and mitochondrial sequences, chimeras of different thallus parts or different cells, and repetitive sequences. Genotypes of heterozygous SNPs were verified by Sanger sequencing of PCR products and monoclones. Duplications of chromosomal rearrangements in the genome of might explain the presence of heterozygous SNPs in haploid gametophytes.
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http://dx.doi.org/10.3389/fgene.2019.01256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915112PMC
December 2019

Phosphate limitation increases coenzyme Q production in industrial HY01.

Synth Syst Biotechnol 2019 Dec 30;4(4):212-219. Epub 2019 Nov 30.

State Key Laboratory of Bioreactor Engineering (SKLBE), And School of Biotechnology, East China University of Science and Technology (ECUST), No. 130 Meilong Road, Shanghai, 200237, China.

Coenzyme Q (CoQ) is an important component of the respiratory chain in humans and some bacteria. As a high-value-added nutraceutical antioxidant, CoQ has excellent capacity to prevent cardiovascular disease. The content of CoQ in the industrial HY01 is hundreds of folds higher than normal physiological levels. In this study, we found that overexpression or optimization of the synthetic pathway failed CoQ overproduction in the HY01 strain. Moreover, under phosphate- limited conditions (decreased phosphate or in the absence of inorganic phosphate addition), CoQ production increased significantly by 12% to220 mg/L, biomass decreased by 12%, and the CoQ productivity of unit cells increased by 27%. In subsequent fed-batch fermentation, CoQ production reached 272 mg/L in the shake-flask fermentation and 1.95 g/L in a 100-L bioreactor under phosphate limitation. Furthermore, to understand the mechanism associated with CoQ overproduction under phosphate- limited conditions, the comparatve transcriptome analysis was performed. These results indicated that phosphate limitation combined with glucose fed-batch fermentation represented an effective strategy for CoQ production in the HY01. Phosphate limitation induced a pleiotropic effect on cell metabolism, and that improved CoQ biosynthesis efficiency was possibly related to the disturbance of energy metabolism and redox potential.
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http://dx.doi.org/10.1016/j.synbio.2019.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909082PMC
December 2019

Construction of discontinuous capillary isoelectric focusing system and its application in pre-fractionation of exosomal proteins.

Talanta 2020 Feb 7;208:119876. Epub 2019 May 7.

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.

Exosomal proteins play an important role in screening disease biomarker and clinic diagnosis. However, low concentration, complex composition and difficulty in extraction hindered the study of their structure and properties. In this study, a new protein pre-fractionation system, discontinuous monolithic immobilized pH gradient capillary isoelectric focusing (DM-IPG-CIEF), was constructed by reassembling pieces of monolithic IPG-CIEF column to create small cavity with well-defined pH range. The monolithic IPG-CIEF column exhibited good linearity (R = 0.9997) and preparation and operation reproducibility. Then removal of high-abundant proteins and enrichment of low-abundant proteins were performed by the DM-IPG-CIEF system successfully. The system was applied to pre-fractionation and enrichment of exosomal proteins extracted from HeLa cells. The exosomal proteins with pI in the range of 6.0-9.0 were focused in enrichment cavity and collected. According to nano-HPLC-MS/MS analysis, 130 proteins in this range were identified, indicating great potential of the DM-IPG-CIEF system in pretreatment of exosomal proteins.
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http://dx.doi.org/10.1016/j.talanta.2019.04.077DOI Listing
February 2020

Evolutionary analysis of six chloroplast genomes from three Persea americana ecological races: Insights into sequence divergences and phylogenetic relationships.

PLoS One 2019 18;14(9):e0221827. Epub 2019 Sep 18.

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.

Chloroplasts significantly influence species phylogenies because of their maternal inheritance and the moderate evolutionary rate of their genomes. Avocado, which is a member of the family Lauraceae, has received considerable attention from botanists, likely because of its position as a basal angiosperm. However, there is relatively little avocado genomic information currently available. In this study, six complete avocado chloroplast genomes from three ecological races were assembled to examine the sequence diversity among the three avocado ecological races. A comparative genomic analysis revealed that 515 simple sequence repeat loci and 176 repeats belonging to four other types were polymorphic across the six chloroplast genomes. Three highly variable regions (trnC-GCA-petN, petN-psbM, and petA-psbJ) were identified as highly informative markers. A phylogenetic analysis based on 79 common protein-coding genes indicated that the six examined avocado accessions from three ecological races form a monophyletic clade. The other three genera belonging to the Persea group clustered to form a sister clade with a high bootstrap value. These chloroplast genomes provide important genetic information for future attempts at identifying avocado races and for the related biological research.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221827PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6750585PMC
March 2020

Metabolomics and Lipidomics Profiling Reveals Hypocholesterolemic and Hypolipidemic Effects of Arabinoxylan on Type 2 Diabetic Rats.

J Agric Food Chem 2019 Sep 17;67(38):10614-10623. Epub 2019 Sep 17.

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang) , Nanchang University , Nanchang 330047 , China.

Type 2 diabetes (T2D) is a pandemic disease chiefly characterized by hyperglycemia. In this study, the combination of serum lipidomic and metabolomic approach was employed to investigate the effect of arabinoxylan on type 2 diabetic rats and identify the critical biomarkers of T2D. Metabolomics analysis revealed that branched-chain amino acids, 12α-hydroxylated bile acids, ketone bodies, and several short- and long-chain acylcarnitines were significantly increased in T2D, whereas lysophosphatidylcholines (LPCs) were significantly decreased. Lipidomics analysis indicated T2D-related dyslipidemia was mainly associated with the increased levels of acetylcarnitine, free fatty acids (FFA), diacylglycerols, triacylglycerols, and cholesteryl esters and the decreased levels of some unsaturated phosphatidylcholines (less than 22 carbons). These variations indicated the disturbed amino acid and lipid metabolism in T2D, and the accumulation of incompletely oxidized lipid species might eventually contribute to impaired insulin action and glucose homeostasis. Arabinoxylan treatment decreased the concentrations of 12α-hydroxylated bile acids, carnitines, and FFAs and increased the levels of LPCs. The improved bile acid and lipid metabolism by arabinoxylan might be involved in the alleviation of hypercholesterolemia and hyperlipidemia in T2D.
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http://dx.doi.org/10.1021/acs.jafc.9b03430DOI Listing
September 2019
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