Publications by authors named "Haifeng Wang"

494 Publications

Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection.

bioRxiv 2021 Jun 9. Epub 2021 Jun 9.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects.
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http://dx.doi.org/10.1101/2021.06.09.447760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202426PMC
June 2021

MicroRNA-181b Inhibits Inflammatory Response and Reduces Myocardial Injury in Sepsis by Downregulating HMGB1.

Inflammation 2021 Jun 2. Epub 2021 Jun 2.

Emergency Department, China-Japan Friendship Hospital, No. 2, Yinghua East Street, Chaoyang District, Beijing, 100029, People's Republic of China.

MicroRNAs (miRNAs) are short endogenous noncoding RNAs regulating protein translation. However, the specific mechanism by which miR-181b influences sepsis via high-mobility group box-1 protein (HMGB1) still remains unknown. Thus, the aim of this study is to investigate the mechanism of miR-181b in regulating inflammatory response in sepsis-induced myocardial injury through targeting high-mobility group box-1 protein (HMGB1). Through cecal ligation and puncture (CLP), the rat model of sepsis was established. Then, the effect of altered expression of miR-181b and HMGB1 on cardiomyocytes was investigated. The positive expression rate of HMGB1, concentration of inflammatory factors, and serum myocardial enzyme of myocardial tissues were determined. Besides, the binding site between miR-181b and HMGB1 was determined by bioinformatics information and dual-luciferase reporter gene assay. The expression of related genes in cells of each group was determined by RT-qPCR and western blot analysis, and the apoptosis rate of transfected cells in each group was determined by TUNEL assay. HMGB1 expression and inflammatory factors were significantly increased in myocardial tissue of rats with sepsis. Cell morphology and the infiltration of inflammatory cells were significantly improved by overexpression of miR-181b. miR-181b directly targeted HMGB1, and downregulation of HMGB1 reduced inflammatory factors and myocardial injury and inhibited cardiomyocyte apoptosis in sepsis. This present study suggests that miR-181b decreased inflammatory factors and reduced myocardial injury in sepsis through downregulation of HMGB1. Thus, a better understanding of this process may aid in the development of novel therapeutic agents in sepsis.
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http://dx.doi.org/10.1007/s10753-020-01411-wDOI Listing
June 2021

Multiple cerebral infarction linked to underlying cancer: a review of Trousseau syndrome-related cerebral infarction.

Br J Hosp Med (Lond) 2021 May 26;82(5):1-7. Epub 2021 May 26.

Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, People's Republic of China.

Trousseau syndrome is a cancer-associated hypercoagulative state leading to venous or arterial thromboembolic events. Cerebral infarction is the most common result of arterial embolism and the pathogenesis is complicated, mainly associated with hypercoagulation and non-bacterial thrombotic endocarditis. On magnetic resonance imaging, multiple lesions are dispersed in more than two territories of the brain, often simultaneously involving the bilateral anterior and posterior circulation. Elevated plasma levels of D-dimer and fibrinogen degradation products may be seen in these patients. There are high rates of short-term recurrence of stroke and sudden death, highlighting the need for early recognition and appropriate treatment of Trousseau syndrome-associated cerebral infarction.
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http://dx.doi.org/10.12968/hmed.2020.0696DOI Listing
May 2021

Confocal Raman microspectral analysis and imaging of the drug response of osteosarcoma to cisplatin.

Anal Methods 2021 Jun;13(22):2527-2536

State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology, Northwest University, #1 Xuefu Avenue, Guodu Education and Technology Industrial Zone Chang'an District, Xi'an, Shaanxi 710127, China.

Confocal Raman microspectral analysis and imaging were used to elucidate the drug response of osteosarcoma (OS) to cisplatin. Raman spectral data were obtained from OS cells that were untreated (UT group) and treated with 20 µM (20T group) and 40 µM (40T group) cisplatin for 24 hours. Statistical analysis of the changes in specific Raman signals was performed using a one-way ANOVA and multiple Tukey's honest significant difference (HSD) post hoc tests. Principal component analysis-linear discriminant analysis (PCA-LDA) was used to highlight the featured cellular drug responses based on the obtained spectral information. For spectral imaging analysis, k-means cluster analysis (KCA) was adopted to clarify the effect of cisplatin dose changes on the subcellular structure and its biochemical composition. The results suggest that the major biochemical changes induced by cisplatin in OS cells undergoing apoptosis are reduced protein and nucleic acid content. Through univariate analysis, the changes in the distribution of nucleic acids in OS cells induced by different doses of cisplatin were obtained. The combination of Raman spectroscopy and multivariate analysis shows that cisplatin mainly acts on the nucleus and causes changes in the secondary structure of proteins. These results indicate that Raman imaging technology has the potential to offer the basis of dose optimization for personalized cancer treatment by helping to understand in vitro cellular drug interactions.
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http://dx.doi.org/10.1039/d1ay00626fDOI Listing
June 2021

Cancer-associated fibroblasts secreted miR-103a-3p suppresses apoptosis and promotes cisplatin resistance in non-small cell lung cancer.

Aging (Albany NY) 2021 05 17;13(10):14456-14468. Epub 2021 May 17.

First Ward of Oncology Department, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China.

Background: The cisplatin resistance of non-small cell lung cancer (NSCLC) patients results in low response rate and overall survival rate. Exosomes contribute to pathological processes of multiple cancers.

Objective: In this study, we explored the function and mechanisms of exosomal miR-103a-3p derived from cancer-associated fibroblast (CAF) in cisplatin resistance in NSCLC.

Results: MiR-103a-3p was highly expressed in CAFs and CAF exosomes, and exosomal miR-103a-3p derived from CAFs in NSCLC. CAFs exosomes co-cultured with NSCLC cells promoted miR-103a-3p expression both in NSCLC cells and its exosomes. Functional experiments showed that exo-miR-103a-3p derived from CAFs promoted cisplatin resistance and inhibited apoptosis in NSCLC cells. Pumilio2 (Pum2) bound with miR-103a-3p in cytoplasm and nucleus, and facilitated packaging into CAF-derived exosomes in NSCLC cells. Further analysis showed Bak1 was a direct target of miR-103a-3p, and miR-103a-3p accelerated cisplatin resistance in NSCLC cells via Bak1 downregulation. tumorigenesis assay showed CAF-derived exosomal miR-103a-3p enhanced cisplatin resistance and inhibited cell apoptosis in NSCLC.

Conclusion: Our study revealed that CAFs-derived exosomal miR-103a-3p promoted cisplatin resistance by suppressing apoptosis via targeting Bak1, which provided a potential therapeutic target for cisplatin resistance in NSCLC.
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http://dx.doi.org/10.18632/aging.103556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202839PMC
May 2021

Risk Factors and Anticoagulation Therapy in Patients With Isolated Distal Deep Vein Thrombosis in the Early Post-operative Period After Thoracic Surgery.

Front Surg 2021 28;8:671165. Epub 2021 Apr 28.

Department of Ultrasound, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Isolated distal deep vein thrombosis (IDDVT) accounts for ~50% of all patients diagnosed with deep venous thrombosis (DVT), but the diagnosis and optimal management of IDDVT remains unclear and controversial. The aim of this study was to explore potential risk factors and predictors of IDDVT, and to evaluate different strategies of anticoagulation therapy. A total of 310 consecutive patients after thoracic surgery, who underwent whole-leg ultrasonography as well as routine measurements of D-dimer levels before and after surgery were evaluated. The general clinical data, anticoagulant therapy, pre- and postoperative D-dimer levels were collected. Differences between IDDVT, DVT and non-DVT groups were calculated. Logistic regression analysis was used to analyze risk factors of postoperative IDDVT. Age and postoperative D-dimer levels were significantly higher in IDDVT group than in non DVT group ( = 0.0053 and < 0.001, respectively). Logistic regression analysis showed that postoperative D-dimer level was a significant independent predictor of IDDVT even when adjusted for age and operation method ( = 0.0003). There were no significant side effects associated with both full-dose and half-dose anticoagulation regimens. Half-dose therapy was associated with a significant decrease in the requirement for anticoagulation medications after discharge ( = 0.0002). Age and D-dimer levels after surgery are strong predictors of IDDVT following thoracic surgery. Half-dose therapeutic anticoagulation has the same efficiency in preventing IDDVT progression, is not associated with any additional risks of adverse effects compared to a full-dose regimen, and may be adopted for treating IDDVT patients after thoracic surgery.
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http://dx.doi.org/10.3389/fsurg.2021.671165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113622PMC
April 2021

Editorial for "Altered Complexity of Spontaneous Brain Activity in Schizophrenia and Bipolar Disorder Patients".

Authors:
Haifeng Wang

J Magn Reson Imaging 2021 May 13. Epub 2021 May 13.

Paul C. Lauterbur Research Centre for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen, Guangdong, China.

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http://dx.doi.org/10.1002/jmri.27672DOI Listing
May 2021

The risk of ischemic stroke in patients with idiopathic inflammatory myopathies: a systematic review and meta-analysis.

Clin Rheumatol 2021 May 1. Epub 2021 May 1.

Department of Neurology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, No. 5 Donghai Middle Road, Qingdao, Shandong Province, 266071, China.

Objective: Idiopathic inflammatory myopathies (IIM) are a group of chronic rheumatic diseases that can affect multiple systems; the risk of ischemic stroke in patients with IIM remains controversial. We aimed to systematically evaluate the risk of ischemic stroke in IIMs.

Methods: The PubMed, Embase, and Cochrane library were searched for relevant studies. Pooled relative risk (RR) with a 95% confidence interval (CI) was calculated as effect size to evaluate the risk of ischemic stroke in patients with IIM. Random effects model was chosen when I > 50%. We pooled all studies in a first total analysis to assess the risk of ischemic stroke in IIM. Subgroup analyses were conducted based on the subtypes of IIM (dermatomyositis) and country. Sensitivity analysis was performed to identify the heterogeneity sources.

Results: A total of 6 studies with 5,114 IIM cases and 14,516,099 controls were included, and the results demonstrated that IIMs were associated with an increased risk of ischemic stroke (RR = 2.41, 95% CI: 1.31, 4.45). Subgroup analysis indicated that patients with dermatomyositis (DM) had a 49% excess risk of ischemic stroke than controls; Asian patients with IIM had an increased risk of ischemic stroke, although not for American patients.

Conclusion: IIMs, especially patients with DM, are associated with an increased risk of ischemic stroke. Appropriate intervention may be taken into account for patients with IIM, especially when accompanied by other traditional risk factors of ischemic stroke. Key Points • This study evaluated the risk of ischemic stroke in patients with IIMs. • Generally, IIMs are associated with an increased risk of ischemic stroke. • Patients with DM had a 49% excess risk of ischemic stroke than controls. • Subgroup analysis showed that Asian patients with IIMs were at increased risk of ischemic stroke, but not Americans.
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http://dx.doi.org/10.1007/s10067-021-05751-2DOI Listing
May 2021

Highly accelerated parallel MRI using wave encoding and virtual conjugate coils.

Magn Reson Med 2021 09 15;86(3):1345-1359. Epub 2021 Apr 15.

Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China.

Purpose: To propose a novel model incorporating virtual conjugate coil (VCC) reconstruction and wave encoding (Wave) for improved parallel MRI.

Theory And Methods: A novel model (VCC-Wave) incorporating VCC and Wave is proposed. The correlation matrix of the encoding operator is introduced to analyze the encoding capability. In addition, simulation experiments are conducted to gain insights into VCC-Wave. In vivo experiments are performed to compare VCC-Wave with alternative methods.

Results: The correlation matrix and the simulation experiments show that the proposed VCC-Wave can utilize more priors of Wave under the VCC framework. In vivo experiments show that the proposed VCC-Wave can achieve good image quality at a 6-fold acceleration in high-resolution and high-bandwidth cases, indicating an improvement over the original Wave technique.

Conclusion: The proposed VCC-Wave can not only combine the advantages of both the VCC and Wave but also exploit more priors of Wave under the VCC framework. The improvement in VCC-Wave alleviates the limitation of Wave in high-resolution and high-bandwidth cases.
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http://dx.doi.org/10.1002/mrm.28803DOI Listing
September 2021

Survival analysis and treatment strategies for limb liposarcoma patients with metastasis at presentation.

Medicine (Baltimore) 2021 Apr;100(13):e25296

Department of Orthopedics, Ningbo No. 6 Hospital, Ningbo.

Abstract: Limited data exist on patients with limb liposarcoma (LLS) with metastasis at presentation Moreover, the potential prognostic factors of this patient population are poorly documented because of its rarity. Therefore, we conducted this study to evaluate the clinicopathologic characteristics and prognostic factors for patients with metastatic LLS.All patients with LLS with metastasis at presentation from 1975 to 2016 were identified by using the Surveillance, Epidemiology, and End Results (SEER) database. The following clinical data were derived from this clinical database: age, sex, histologic grade, subtype, size of tumor, surgery, radiotherapy, chemotherapy, vital status, cause of death, and survival duration. The Kaplan-Meier method was performed to calculate median survival time and draw survivorship curves. Cox-proportional hazards regression model was used to reveal the statistical independence between various variables.The present study collected 184 cases from SEER database for survival analysis. Mean age was 57.8 years with 63.6% (n = 117) men. The 3-year overall survival (OS) and cancer-specific survival (CSS) rates of this population were 27.8% and 30.1%, respectively. Univariate analysis revealed that age, tumor grade, and surgery were significantly correlated with survival. Sex and tumor size did not reach significant predictor status of survival. Multivariate analysis revealed that age at diagnosis <60, low tumor grade, and local surgery were significantly correlated with improved OS and CSS.Patients with LLS with metastasis at diagnosis experienced quite poor prognosis. Currently, surgery for the primary tumor significantly prolonged the survival of those patients, whereas chemotherapy and radiotherapy need to be further confirmed.
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http://dx.doi.org/10.1097/MD.0000000000025296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021344PMC
April 2021

Biased Symmetry Breaking and Chiral Control by Self-Replicating in Achiral Tetradentate Platinum (II) Complexes.

Angew Chem Int Ed Engl 2021 May 6;60(19):10531-10536. Epub 2021 Apr 6.

College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, 610000, P. R. China.

Obtaining homochirality from biased symmetry-breaking of self-assembly in achiral molecules remains a great challenge due to the lack of ingenious strategies and controlling their handedness. Here, we report the first case of biased symmetry breaking from achiral platinum (II) liquid crystals which self-organize into an enantiomerically enriched single domain without selection of handedness in twist grain boundary TGB * phase. Most importantly, the chiral control of self-organization can be achieved by using above the homochiral liquid crystal films with determined handedness (P or M) as a template. Moreover, benefiting from self-assembled superhelix, these complexes exhibit prominent circularly polarized luminescence with high |g | up to 3.4×10 in the TGB * mesophase. This work paves a neoteric avenue for the development of chiral self-assemblies from achiral molecules.
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http://dx.doi.org/10.1002/anie.202101709DOI Listing
May 2021

Automatic Detection of Scattered Garbage Regions Using Small Unmanned Aerial Vehicle Low-Altitude Remote Sensing Images for High-Altitude Natural Reserve Environmental Protection.

Environ Sci Technol 2021 03 1;55(6):3604-3611. Epub 2021 Mar 1.

The Engineering Research Center of GIS Technology in Western China of Ministry of Education, Kunming 650500, China.

Recently, some famous high-altitude nature reserves have been shut down due to tourist garbage pollution. In order to clean up such garbage more conveniently and quickly, a novel detection framework is proposed to automatically detect scattered garbage regions using low-altitude remote sensing of small unmanned aerial vehicles (SUAVs), and it contains the following steps. First, high-resolution, low-altitude, multitemporal remote sensing images containing scattered garbage regions are collected by SUAVs, and two data augmentation methods are proposed to expand the training samples. Second, low-altitude remote sensing image registration and target-level image change detection are used to extract the candidate regions of garbage. Finally, a deep learning detection network is adopted to classify the scattered garbage regions. Experimental results show that the proposed detection framework achieves a mean accuracy of 96.94% and provides better performances on the real dataset compared with state-of-the-art methods.
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http://dx.doi.org/10.1021/acs.est.0c04068DOI Listing
March 2021

Inhibition of the mevalonate pathway improves myocardial fibrosis.

Exp Ther Med 2021 Mar 18;21(3):224. Epub 2021 Jan 18.

Department of Cardiology, Tongji Hospital Affiliated to Tongji University, Shanghai 200065, P.R. China.

The mevalonate (MVA) pathway serves an important role in ventricular remodeling. Targeting the MVA pathway has protective effects against myocardial fibrosis. The present study aimed to investigate the mechanism behind these effects. Primary cultured cardiac fibroblasts from C57BL/6 mice were treated in 5 groups: i) negative control; ii) angiotensin II (Ang II) model (1x10 mol/l); iii) Ang II + rosuvastatin (ROS); iv) Ang II + alendronate (ALE); and v) Ang II + fasudil (FAS). Collagen and crystal violet staining were used to assess morphological changes in cardiac fibroblasts. Reverse transcription quantitative PCR and western blotting were used to analyze the expression of key signaling molecules involved in the MVA pathway. Collagen staining in the ALE, FAS, and ROS groups was weak compared with the Ang II group, while the rate of cell proliferation in the ROS, ALE, and FAS groups was slower compared with that in the Ang II group. In addition, the expression of key signaling molecules in the MVA pathway, including transforming growth factor-β1 (), heat shock protein 47 (), collagen type I α1 (), vascular endothelial growth factor 2 () and fibroblast growth factor 2 (), was decreased in the FAS and ROS groups compared with the Ang II model. Compared with the Ang II group, 3-Hydroxy-3-Methylglutaryl-CoA reductase () gene expression was significantly lowered in the drug intervention groups, whereas farnesyl pyrophosphate synthase () expression was downregulated in the ALE group, but elevated in the FAS and ROS groups. Compared with that in the Ang II group, ras homolog family member A () expression was downregulated in the FAS and ROS groups, whilst mevalonate kinase expression was reduced in the ROS group. Protein expression of TGF-β1, COL1A1 and HSP47 were decreased following intervention with each of the three drugs compared with the Ang II group. Overall, rosuvastatin, aledronate and fasudil decreased the proliferation of myocardial fibroblasts and inhibited collagen synthesis. Rosuvastatin had the strongest protective effects against myocardial fibrosis compared with the other drugs tested, suggesting this to be a potential agent for the clinical treatment of cardiovascular disease.
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http://dx.doi.org/10.3892/etm.2021.9655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851600PMC
March 2021

Engineering 3D genome organization.

Nat Rev Genet 2021 Jun 8;22(6):343-360. Epub 2021 Feb 8.

Department of Bioengineering, Stanford University, Stanford, CA, USA.

Cancers and developmental disorders are associated with alterations in the 3D genome architecture in space and time (the fourth dimension). Mammalian 3D genome organization is complex and dynamic and plays an essential role in regulating gene expression and cellular function. To study the causal relationship between genome function and its spatio-temporal organization in the nucleus, new technologies for engineering and manipulating the 3D organization of the genome have been developed. In particular, CRISPR-Cas technologies allow programmable manipulation at specific genomic loci, enabling unparalleled opportunities in this emerging field of 3D genome engineering. We review advances in mammalian 3D genome engineering with a focus on recent manipulative technologies using CRISPR-Cas and related technologies.
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http://dx.doi.org/10.1038/s41576-020-00325-5DOI Listing
June 2021

Study protocol for a single-centre non-inferior randomised controlled trial on a novel three-dimensional matrix positioning-based cognitive fusion-targeted biopsy and software-based fusion-targeted biopsy for the detection rate of clinically significant prostate cancer in men without a prior biopsy.

BMJ Open 2021 02 5;11(2):e041427. Epub 2021 Feb 5.

Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China

Introduction: The classical pathway for diagnosing prostate cancer is systematic 12-core biopsy under the guidance of transrectal ultrasound, which tends to underdiagnose the clinically significant tumour and overdiagnose the insignificant disease. Another pathway named targeted biopsy is using multiparametric MRI to localise the tumour precisely and then obtain the samples from the suspicious lesions. Targeted biopsy, which is mainly divided into cognitive fusion method and software-based fusion method, is getting prevalent for its good performance in detecting significant cancer. However, the preferred targeted biopsy technique in detecting clinically significant prostate cancer between cognitive fusion and software-based fusion is still beyond consensus.

Methods And Analysis: This trial is a prospective, single-centre, randomised controlled and non-inferiority study in which all men suspicious to have clinically significant prostate cancer are included. This study aims to determine whether a novel three-dimensional matrix positioning cognitive fusion-targeted biopsy is non-inferior to software-based fusion-targeted biopsy in the detection rate of clinically significant cancer in men without a prior biopsy. The main inclusion criteria are men with elevated serum prostate-specific antigen above 4-20 ng/mL or with an abnormal digital rectal examination and have never had a biopsy before. A sample size of 602 participants allowing for a 10% loss will be recruited. All patients will undergo a multiparametric MRI examination, and those who fail to be found with a suspicious lesion, with the anticipation of half of the total number, will be dropped. The remaining participants will be randomly allocated to cognitive fusion-targeted biopsy (n=137) and software-based fusion-targeted biopsy (n=137). The primary outcome is the detection rate of clinically significant prostate cancer for cognitive fusion-targeted biopsy and software-based fusion-targeted biopsy in men without a prior biopsy. The clinically significant prostate cancer will be defined as the International Society of Urological Pathology grade group 2 or higher.

Ethics And Dissemination: Ethical approval was obtained from the ethics committee of Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. The results of the study will be disseminated and published in international peer-reviewed journals.

Trial Registration Number: ClinicalTrials.gov Registry (NCT04271527).
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http://dx.doi.org/10.1136/bmjopen-2020-041427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925935PMC
February 2021

A graphical user interface (NWUSA) for Raman spectral processing, analysis and feature recognition.

J Biophotonics 2021 May 18;14(5):e202000456. Epub 2021 Feb 18.

State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology, Northwest University, Xi'an, Shaanxi, China.

It is a practical necessity for non-professional users to interpret biologically derived Raman spectral information for obtaining accurate and reliable analytical results. An integrated Raman spectral analysis software (NWUSA) was developed for spectral processing, analysis, and feature recognition. It provides a user-friendly graphical interface to perform the following preprocessing tasks: spectral range selection, cosmic ray removal, polynomial fitting based background subtraction, Savitzky-Golay smoothing, area-under-curve normalization, mean-centered procedure, as well as multivariate analysis algorithms including principal component analysis (PCA), linear discriminant analysis, partial least squares-discriminant analysis, support vector machine (SVM), and PCA-SVM. A spectral dataset obtained from two different samples was utilized to evaluate the performance of the developed software, which demonstrated that the analysis software can quickly and accurately achieve functional requirements in spectral data processing and feature recognition. Besides, the open-source software can not only be customized with more novel functional modules to suit the specific needs, but also benefit many Raman based investigations, especially for clinical usages.
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http://dx.doi.org/10.1002/jbio.202000456DOI Listing
May 2021

Transrectal Ultrasound-Guided Transperineal Puncture: A Viable Alternative for Difficult Removal of an Indwelling Catheter in a Postrobot-Assisted Laparoscopic Radical Prostatectomy Patient.

J Endourol Case Rep 2020 29;6(4):516-518. Epub 2020 Dec 29.

Department of Urology, Shanghai East Hospital, Shanghai, China.

The inability to remove an indwelling urethral catheter in a postrobot-assisted laparoscopic radical prostatectomy (RALP) patient constitutes a serious problem to the urologist. If the proper deflation of the catheter balloon is not observed, forcible extraction can lead to devastating consequences such as urethral disruption and subsequent stricture formation. A 60-year-old male patient developed lower urinary-tract symptoms 20 months after robotic prostatectomy for early prostate cancer. Cystourethroscopy revealed a migrated Hemo-lok clip that was extracted near the anastomotic site, followed by insertion of an indwelling Foley catheter. Two weeks later, the patient accidentally pulled the catheter into the urethra. Several attempts were done to deflate the catheter, which failed. Subsequently, a transrectal ultrasound (TRUS)-guided transperineal puncture was done to deflate the catheter balloon followed by effective catheter removal. TRUS-guided transperineal puncture (under local anesthesia) of an indwelling catheter balloon is a viable alternative for patients who have a history of RALP.
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http://dx.doi.org/10.1089/cren.2019.0147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803189PMC
December 2020

SSIA: A sensitivity-supervised interlock algorithm for high-performance microkinetic solving.

J Chem Phys 2021 Jan;154(2):024108

Key Laboratory for Advanced Materials, Centre for Computational Chemistry and Research Institute of Industrial Catalysis, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.

Microkinetic modeling has drawn increasing attention for quantitatively analyzing catalytic networks in recent decades, in which the speed and stability of the solver play a crucial role. However, for the multi-step complex systems with a wide variation of rate constants, the often encountered stiff problem leads to the low success rate and high computational cost in the numerical solution. Here, we report a new efficient sensitivity-supervised interlock algorithm (SSIA), which enables us to solve the steady state of heterogeneous catalytic systems in the microkinetic modeling with a 100% success rate. In SSIA, we introduce the coverage sensitivity of surface intermediates to monitor the low-precision time-integration of ordinary differential equations, through which a quasi-steady-state is located. Further optimized by the high-precision damped Newton's method, this quasi-steady-state can converge with a low computational cost. Besides, to simulate the large differences (usually by orders of magnitude) among the practical coverages of different intermediates, we propose the initial coverages in SSIA to be generated in exponential space, which allows a larger and more realistic search scope. On examining three representative catalytic models, we demonstrate that SSIA is superior in both speed and robustness compared with its traditional counterparts. This efficient algorithm can be promisingly applied in existing microkinetic solvers to achieve large-scale modeling of stiff catalytic networks.
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http://dx.doi.org/10.1063/5.0032228DOI Listing
January 2021

A 4-minute solution for submillimeter whole-brain T quantification.

Magn Reson Med 2021 06 9;85(6):3299-3307. Epub 2021 Jan 9.

Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.

Purpose: To develop a robust, accurate, and accelerated T quantification solution for submillimeter in vivo whole-brain imaging.

Methods: A multislice T mapping solution (MS-T ) was developed based on a two-acquisition scheme using turbo spin echo with RF cycling to allow for whole-brain coverage with 0.8-mm in-plane resolution. A compressed sensing-based fast imaging method, SCOPE, was used to accelerate the MS-T acquisition time to a total scan time of 3 minutes 31 seconds. A phantom experiment was conducted to assess the accuracy of MS-T by comparing the T value obtained using MS-T with the reference value obtained using the standard single-slice T mapping method. In vivo scans of 13 volunteers were acquired prospectively to validate the robustness of MS-T .

Results: In the phantom study, the T values obtained with MS-T were in good agreement with the reference T values (R = 0.9991) and showed high consistency throughout all slices (coefficient of variation = 2.2 ± 2.43%). In the in vivo experiments, T maps were successfully acquired for all volunteers with no visually noticeable artifacts. There was no significant difference in T values between MS-T acquisitions and fully sampled acquisitions for all brain tissues (p-value > .05). In the intraclass correlation coefficient and Bland-Altman analyses, the accelerated T measurements show moderate to good agreement to the fully sampled reference values.

Conclusion: The proposed MS-T solution allows for high-resolution whole-brain T mapping within 4 minutes and may provide a potential tool for investigating neural diseases.
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http://dx.doi.org/10.1002/mrm.28656DOI Listing
June 2021

Integrated analysis of DNA methylome and transcriptome reveals epigenetic regulation of CAM photosynthesis in pineapple.

BMC Plant Biol 2021 Jan 6;21(1):19. Epub 2021 Jan 6.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

Background: Crassulacean acid metabolism (CAM) photosynthesis is an important carbon fixation pathway especially in arid environments because it leads to higher water-use efficiency compared to C3 and C4 plants. However, the role of DNA methylation in regulation CAM photosynthesis is not fully understood.

Results: Here, we performed temporal DNA methylome and transcriptome analysis of non-photosynthetic (white base) and photosynthetic (green tip) tissues of pineapple leaf. The DNA methylation patterns and levels in these two tissues were generally similar for the CG and CHG cytosine sequence contexts. However, CHH methylation was reduced in white base leaf tissue compared with green tip tissue across diel time course in both gene and transposon regions. We identified thousands of local differentially methylated regions (DMRs) between green tip and white base at different diel periods. We also showed that thousands of genes that overlapped with DMRs were differentially expressed between white base and green tip leaf tissue across diel time course, including several important CAM pathway-related genes, such as beta-CA, PEPC, PPCK, and MDH.

Conclusions: Together, these detailed DNA methylome and transcriptome maps provide insight into DNA methylation changes and enhance our understanding of the relationships between DNA methylation and CAM photosynthesis.
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http://dx.doi.org/10.1186/s12870-020-02814-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789485PMC
January 2021

Glyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of to Host Mucin to Enhance Probiotic Effects.

Int J Mol Sci 2020 Dec 21;21(24). Epub 2020 Dec 21.

College of Animal Science, Zhejiang University, Hangzhou 310058, China.

The ability to adhere to the intestinal mucus layer is an important property of probiotic bacteria. strains ZJ615 and ZJ617 show low and high adhesion, respectively, to intestinal epithelial cells. In this study, we quantified bacterial cell wall-associated glyceraldehyde-3-phosphate dehydrogenases (cw-GAPDH) and bacterial cell membrane permeability in both strains using immunoblotting and flow cytometry, respectively. Highly adhesive ZJ617 possessed significantly more cw-GAPDH, higher cell membrane permeability, and significantly higher adhesive ability toward mucin compared with low-adhesive ZJ615. In vitro adhesion studies and analysis of interaction kinetics using the Octet, the system revealed significantly decreased interaction between and mucin when mucin was oxidized when bacterial surface proteins were removed when bacteria were heat-inactivated at 80 °C for 30 min, and when the interaction was blocked with an anti-GAPDH antibody. SWISS-MODEL analysis suggested intensive interactions between mucin glycans (GalNAcα1-O-Ser, GalNAcαSer, and Galβ3GalNAc) and GAPDH. Furthermore, in vivo studies revealed significantly higher numbers of bacteria adhering to the jejunum, ileum, and colon of piglets orally inoculated with ZJ617 compared with those inoculated with ZJ615; this led to a significantly decreased rate of diarrhea in piglets inoculated with ZJ617. In conclusion, there are strong correlations among the abundance of cw-GAPDH in , the ability of the bacterium to adhere to the host, and the health benefits of this probiotic.
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http://dx.doi.org/10.3390/ijms21249756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766874PMC
December 2020

Assessing health-related quality of life of living kidney donors using the 36-item medical outcomes Short-Form-36 questionnaire: a meta-analysis.

Psychol Health Med 2020 Dec 17:1-14. Epub 2020 Dec 17.

Xiangya School of Nursing, Central South University, Changsha, China.

The purpose of this meta-analysis was to assess the health-related quality of life (HRQoL) of living kidney donors using the 36-item Medical Outcomes Short-Form-36 questionnaire (SF-36). A systematic search of the Web of Science, PubMed, Embase, Elsevier/ScienceDirect, Wanfang, Weipu, and China National Knowledge Infrastructure databases for studies that used the SF-36 to evaluate the HRQoL of living kidney donors up to April 2020 was performed. Stata version 12.0 (StataCorp LLC, College Station, TX, USA) was used for meta-analysis. In all, nine studies comprising 802 living kidney donors were included in this meta-analysis. The research revealed that living kidney donors were inferior in physical health to the general population with regard to bodily pain (BP), superior to the general population in terms of general health (GH), and exhibited no significant difference from the general population in physical function (PF) and role-physical (RP). In the case of psychological health, living kidney donation had a positive impact on well-screened living kidney donors. Based on our results, clinicians can inform potential kidney donors that there is a low risk in donating a kidney, which contributes to provide guidance to design counseling interventions for both kidney recipients and donors.
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http://dx.doi.org/10.1080/13548506.2020.1856392DOI Listing
December 2020

CATKINAS: A large-scale catalytic microkinetic analysis software for mechanism auto-analysis and catalyst screening.

J Comput Chem 2021 Feb 14;42(5):379-391. Epub 2020 Dec 14.

Key Laboratory for Advanced Materials, Centre for Computational Chemistry and Research Institute of Industrial Catalysis, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

As an effective method to analyze complex catalytic reaction networks, microkinetic modeling is gaining increasing popularity in the catalytic activity evaluation and rational design of heterogeneous catalysts. An automated simulator with stable and reliable performance is especially useful and in great request. Here we introduce the CATKINAS package developed for large-scale microkinetic modeling and analysis. Featuring with a multilevel solver and a multifunctional analyzer, CATKINAS can provide both accurate solutions and various quantitative and automatic analysis for a wide range of catalytic systems. The structure and the basic workflow are overviewed with the multilevel solver particularly illustrated. Also, we take the CO methanation reaction as an example to illustrate the application and efficiency of the CATKINAS package.
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http://dx.doi.org/10.1002/jcc.26464DOI Listing
February 2021

Predictors and one-year outcomes of patients with delayed graft function after deceased donor kidney transplantation.

BMC Nephrol 2020 12 4;21(1):526. Epub 2020 Dec 4.

Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha, Hunan, 410011, China.

Background: Delayed graft function (DGF) is closely associated with the use of marginal donated kidneys due to deficits during transplantation and in recipients. We aimed to predict the incidence of DGF and evaluate its effect on graft survival.

Methods: This retrospective study on kidney transplantation was conducted from January 1, 2018, to December 31, 2019, at the Second Xiangya Hospital of Central South University. We classified recipients whose operations were performed in different years into training and validation cohorts and used data from the training cohort to analyze predictors of DGF. A nomogram was then constructed to predict the likelihood of DGF based on these predictors.

Results: The incidence rate of DGF was 16.92%. Binary logistic regression analysis showed correlations between the incidence of DGF and cold ischemic time (CIT), warm ischemic time (WIT), terminal serum creatine (Scr) concentration, duration of pretransplant dialysis, primary cause of donor death, and usage of LifePort. The internal accuracy of the nomogram was 83.12%. One-year graft survival rates were 93.59 and 99.74%, respectively, for the groups with and without DGF (P < 0.05).

Conclusion: The nomogram established in this study showed good accuracy in predicting DGF after deceased donor kidney transplantation; additionally, DGF decreased one-year graft survival.
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http://dx.doi.org/10.1186/s12882-020-02181-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716446PMC
December 2020

Towards the Circular Economy: Converting Aromatic Plastic Waste Back to Arenes over a Ru/Nb O Catalyst.

Angew Chem Int Ed Engl 2021 Mar 18;60(10):5527-5535. Epub 2021 Jan 18.

Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore.

The upgrading of plastic waste is one of the grand challenges for the 21 century owing to its disruptive impact on the environment. Here, we show the first example of the upgrading of various aromatic plastic wastes with C-O and/or C-C linkages to arenes (75-85 % yield) via catalytic hydrogenolysis over a Ru/Nb O catalyst. This catalyst not only allows the selective conversion of single-component aromatic plastic, and more importantly, enables the simultaneous conversion of a mixture of aromatic plastic to arenes. The excellent performance is attributed to unique features including: (1) the small sized Ru clusters on Nb O , which prevent the adsorption of aromatic ring and its hydrogenation; (2) the strong oxygen affinity of NbO species for C-O bond activation and Brønsted acid sites for C-C bond activation. This study offers a catalytic path to integrate aromatic plastic waste back into the supply chain of plastic production under the context of circular economy.
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http://dx.doi.org/10.1002/anie.202011063DOI Listing
March 2021

Recent advances in pretreating technology for recycling valuable metals from spent lithium-ion batteries.

J Hazard Mater 2021 Mar 21;406:124332. Epub 2020 Oct 21.

Advanced Analysis and Computation Center, China University of Mining and Technology, No.1 Daxue Road, Jiangsu, Xuzhou 221116, China.

In recent years, the amount of spent lithium-ion batteries (LIBs) increase sharply due to the promotion of new energy vehicles and the limited service life. Recycling of spent LIBs has attracted much attention because of the serious environmental pollution and high economic value. Although some established techniques have been presented in spent LIBs recycling process, but most of them focus on cathode material recycling due to its high economic value. Therefore, preparation of high purity cathode material by a proper pretreating technology is an important procedure. In this paper, the technologies used in the pretreating process of spent LIBs are summarized systematically from three main points of discharging procedure, liberation, and separation. The collaborative application of multi-technologies is the key to realize efficient pretreating process, which can lay the foundation for the subsequent metallurgical process. In addition, an alternative pretreating flowchart of spent LIBs is proposed based on the multi-process collaboration. Pretreating procedures in this process are mainly based on the physical property difference, and they include "Discharging-Shredding-Crushing-Sieving-Separation".
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http://dx.doi.org/10.1016/j.jhazmat.2020.124332DOI Listing
March 2021

A general doping rule: rational design of Ir-doped catalysts for the oxygen evolution reaction.

Chem Commun (Camb) 2020 Dec;56(96):15201-15204

Key Laboratory for Advanced Materials, Centre for Computational Chemistry and Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237, China.

Revealing how to rationally select a suitable dopant or the host counterpart is greatly important for optimizing the catalytic activity of transition metal oxides (TMOs). We systematically report the adsorption trends of atomic O and H, two selected representative adsorbates, on numerous doped rutile-type TMO surfaces under two different doping modes, aiming to demonstrate adsorption energy (AE) variations of different adsorbates across doped TMO surfaces. A "host-guest interaction" induced adsorption tuning rule for the ternary doping systems is identified, which rationally directs the proposal of cost-effective Ir-doped CrO2 or MnO2 catalysts for the electrocatalytic oxygen evolution reaction compared with the common IrO2.
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http://dx.doi.org/10.1039/d0cc06282kDOI Listing
December 2020

Long-term outcomes of laparoscopic versus open donor nephrectomy for kidney transplantation: a meta-analysis.

Am J Transl Res 2020 15;12(10):5993-6002. Epub 2020 Oct 15.

Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University Changsha, China.

Laparoscopic surgery is widely used for living donor nephrectomy and has demonstrated superiority over open surgery by improving several outcomes, such as length of hospital stay and morphine requirements. The purpose of the present study was to compare the long-term outcomes of open donor nephrectomy (ODN) versus laparoscopic donor nephrectomy (LDN) using meta-analytical techniques. The Web of Science, PubMed and Cochrane Library databases were searched, for relevant articles published between 1980 and January 20, 2020. Lists of reference articles retrieved in primary searches were manually screened for potentially eligible studies. Outcome parameters were explored using Review Manager version 5.3. The evaluated outcomes included donor serum creatinine levels, incidence of hypertension or proteinuria at 1 year postoperative, donor health-related quality of life, donation attitude, and graft survival. Thirteen of the 111 articles fulfilled the inclusion criteria. The LDN group demonstrated similar 1 year outcomes compared with ODN with respect to serum creatinine levels (weighted mean difference [WMD] -0.02 mg/dL [95% confidence interval (CI) -0.18-0.13]; =0.77); hypertension (odds ratio [OR] 1.21 [95% CI 0.48-3.08]; =0.68); proteinuria (OR 0.28 [95% CI 0.02-3.11]; =0.30); and donation attitude (OR 4.26 [95% CI 0.06-298.27]; =0.50). Donor health-related quality of life and recipient graft survival were also not significantly different between the groups analyzed. Thus, the long-term outcomes between LDN and ODN for living donor kidney transplantation are similar.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653626PMC
October 2020

A critical review of current technologies for the liberation of electrode materials from foils in the recycling process of spent lithium-ion batteries.

Sci Total Environ 2021 Apr 16;766:142382. Epub 2020 Sep 16.

Guangdong Guanghua Technology Co., Ltd., No.295 University Road, Shantou, Guangdong 515063, China.

Proper disposal of spent lithium-ion batteries is beneficial for the resource recycling and pollution elimination. Full liberation of electrode materials, including the liberation between electrode material and current collector (copper/aluminum foils) and the liberation among electrode material particles, is the pivotal precondition for improving the recovery efficiency of electrode materials. In this article, authors attempt to carry out a summary of current technologies used in the liberation of electrode materials derived from spent lithium-ion batteries. However, specialized studies about the liberation of electrode materials are insufficient at present. This research clearly shows that: (1) Organic binder must be removed so as to improve the liberation and metallurgy efficiency of electrode materials; (2) A collaboration of varied technologies is the necessary process to achieve high liberation efficiency between electrode materials and copper/aluminum foils; (3) Pyrolysis may be a recommended technology for removal of organic binder because part of pyrolysis products can be recovered. Finally, an alternative recycling flowchart of spent LIBs is proposed.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142382DOI Listing
April 2021

Digital PCR is a sensitive new technique for SARS-CoV-2 detection in clinical applications.

Clin Chim Acta 2020 Dec 4;511:346-351. Epub 2020 Nov 4.

Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China. Electronic address:

The global coronavirus disease 2019 (COVID-19) pandemic has posed great challenges in people's daily lives. Highly sensitive laboratory techniques played a critical role in clinical COVID-19 diagnosis and management. In this study the feasibility of using a new digital PCR-based detection assay for clinical COVID-19 diagnosis was investigated by comparing its performance with that of RT-PCR. Clinical patient samples and samples obtained from potentially contaminated environments were analyzed. The study included 10 patients with confirmed COVID-19 diagnoses, 32 validated samples of various types derived from different clinical timepoints and sites, and 148 environmentally derived samples. SARS-CoV-2 nucleic acids were more readily detected in respiratory tract samples (35.0%). In analyses of environmentally derived samples, the positivity rate of air samples was higher than that of surface samples, probably due to differences in virus concentrations. Digital PCR detected SARS-CoV-2 in several samples that had previously been deemed negative, including 3 patient-derived samples and 5 environmentally derived samples. In this study digital PCR exhibited higher sensitivity than conventional RT-PCR, suggesting that it may be a useful new method for clinical SARS-CoV-2 detection. Improvement of SARS-CoV-2 detection would substantially reduce the rates of false-negative COVID-19 test results, in particular those pertaining to asymptomatic carriers.
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http://dx.doi.org/10.1016/j.cca.2020.10.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641518PMC
December 2020