Publications by authors named "Haidong Liu"

52 Publications

Selective C-F Bond Allylation of Trifluoromethylalkenes.

Angew Chem Int Ed Engl 2021 Jun 30. Epub 2021 Jun 30.

Technical Institute of Fluorochemistry (TIF), Institute of Advanced Synthesis (IAS), School of Chemistry and Molecular Engineering, Nanjing Tech University, 30 South Puzhu Road, Nanjing, 211816, P. R. China.

Selective C-F bond functionalization of CF group represents an appealing strategy for the incorporation of pharmaceutically privileged difluoromethylene moiety. Despite the recent significant advancement attained in the functionalization of Ar-CF molecules, prescriptions amenable for alkenyl-CF congeners remain sufficiently inadequate. Herein, we report a strategically novel protocol for the C-F bond elaboration of trifluoromethylalkene derivatives. By using readily available allyl metallics as nucleophilic coupling partner, the present reaction enables an expedient construction of structurally diversified CF -bridged 1,5-dienes. Furthermore, the exquisite selectivity observed in this transformation is revealed to be based on the underlying mechanism that consists of a cascade of nucleophilic S 2' defluorinative allylation and electronically promoted Cope rearrangement.
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http://dx.doi.org/10.1002/anie.202106531DOI Listing
June 2021

Synthesis of the Platinum Nanoribbons Regulated by Fluorine and Applications in Electrocatalysis.

Inorg Chem 2021 Apr 25;60(7):4366-4370. Epub 2021 Mar 25.

Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science & Collaborative Innovation Centre of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, P. R. China.

Controlling the morphology of highly homogeneous nanoribbons is one of the main goals for synthesizing catalysts with excellent activity and durability. In this Communication, platinum (Pt) nanoribbons were synthesized by a one-pot method. We used ammonium fluoride (NHF) as the regulator, under 8 atm of hydrogen (H), to synthesize zigzag-shaped two-dimensional Pt nanoribbons. Benefiting from their unique morphology, the Pt nanoribbons display superior electrocatalytic activity and stability.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00231DOI Listing
April 2021

SNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA.

Cancer Biol Med 2021 Mar 12. Epub 2021 Mar 12.

Department of Laboratory Diagnostics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.

Objective: The dysregulation of ribosome biogenesis is associated with the progression of numerous tumors, including hepatocellular carcinoma (HCC). Small nucleolar RNAs (snoRNAs) regulate ribosome biogenesis by guiding the modification of ribosomal RNAs (rRNAs). However, the underlying mechanism of this process in HCC remains elusive.

Methods: RNA immunoprecipitation and sequencing were used to analyze RNAs targeted by ribosome proteins. The biological functions of SNORA23 were examined in HCC cells and a xenograft mouse model. To elucidate the underlying mechanisms, the 2'-O-ribose methylation level of rRNAs was evaluated by qPCR, and the key proteins in the PI3K/Akt/mTOR pathway were detected using Western blot.

Results: Twelve snoRNAs were found to co-exist in 4 cancer cell lines using RPS6 pull-down assays. SNORA23 was downregulated in HCC and correlated with the poor prognoses of HCC patients. SNORA23 inhibited the proliferation, migration, and invasion of HCC cells both and . We also found that SNORA23 regulated ribosome biogenesis by impairing 2'-O-ribose methylation of cytidine of 28S rRNA. Furthermore, SNORA23, which is regulated by the PI3K/Akt/mTOR signaling pathway, significantly inhibited the phosphorylation of 4E binding protein 1. SNORA23 and rapamycin blocked the PI3K/AKT/mTOR signaling pathway and impaired HCC growth .

Conclusions: SNORA23 exhibited antitumor effects in HCC and together with rapamycin, provided a promising therapeutic strategy for HCC treatment.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0343DOI Listing
March 2021

One-pot Synthesis of Pd/Azo-polymer as an Efficient Catalyst for 4-Nitrophenol Reduction and Suzuki-Miyaura Coupling Reaction.

Chem Asian J 2021 Apr 10;16(7):837-844. Epub 2021 Mar 10.

Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, 215123, P. R. China.

The porous polymer matrix with good stability and confined microenvironment is considered as ideal support to stabilize isolated metal centers for catalysis. Herein, we report a "one-pot" method to prepare a kind of palladium complexed with azo porous organic polymer nanospheres (Pd-azo-POPs). The method combines the synthesis of azo-POPs with the reduction of the Pd ion, where azo serves as an anchoring group to limit the growth of Pd. The unique structure is conductive to the formation of a uniform active center and provides improved electron transfer. Pd-azo-POPs-80 exhibits a high catalytic activity and cycling stability both in 4-nitrophenol reduction and Suzuki-Miyaura coupling. The k for the 4-nitrophenol reduction was 174.7 min  mM and the conversion remains above 90% after 6 cycles. Meanwhile, the yield was still up to 94.5% after 5 cycles for the Suzuki-Miyaura coupling reaction of benzene derivatives with I/Br under mild conditions.
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http://dx.doi.org/10.1002/asia.202100002DOI Listing
April 2021

Ultrathin amorphous iron-doped cobalt-molybdenum hydroxide nanosheets for advanced oxygen evolution reactions.

Nanoscale 2021 Feb 2;13(5):3153-3160. Epub 2021 Feb 2.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Jiangsu, 215123, P. R. China.

Developing the highly efficient and low-cost electrocatalysts for the oxygen evolution reactions (OERs), as vital half reactions of water splitting, is crucial for renewable energy technology. The electrocatalysts based on multi-component and hierarchically structured non-noble metal hydr(oxy)oxide materials are of great prospects. Herein, we report an efficient strategy at low temperatures for synthesizing amorphous iron-doped cobalt-molybdenum ultrathin hydroxide (Fe-CoMo UH) nanosheets. Benefiting from the ultrathin amorphous structure and multi-metal coordination, Fe-CoMo UH nanosheets exhibit outstanding performance for OERs with a low overpotential of 245 mV at 10 mA cm, a small Tafel slope of 37 mV dec and an excellent stability for 90 h. The mass activity of Fe-CoMo UH is higher than that of commercial Ir/C and most of the transition metal hydroxide catalysts. This work provides a feasible consideration for the construction of promising efficient non-noble metal catalysts.
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http://dx.doi.org/10.1039/d0nr08408eDOI Listing
February 2021

Population genetic characteristics for populations on the Shandong Peninsula revealed by autosomal STR.

Ann Hum Biol 2021 Mar;48(2):157-159

Forensic Center, College of Medicine, Shaoxing University, Shaoxing, China.

The Shandong Peninsula is the largest peninsula in China and has played a vital part in Chinese civilisation. The ancient independent Laizi kingdom was located on the Shandong peninsula. While large demographic changes have happened at this peninsula throughout history, the genetic landscape of modern populations on this peninsula has never been clarified. The aims of our study were to investigate population genetic characteristics of the populations on the Shandong peninsula and to reveal their genetic affinities with other populations around the world. Allele frequencies, Hardy-Weinberg equilibrium, and forensic parameters of 15 autosomal STRs in the AmpFSTR Identifiler system were obtained from the studied populations with 2441 individuals in total. Allele frequencies were used to reveal the phylogenetic relationships among 287 worldwide populations. The combined power of discrimination (CPD) and the combined power of exclusion (CPE) in the whole of the Han population on the Shandong Peninsula were 0.999999999999999983 and 0.999998155, respectively. The 15 autosomal loci were polymorphic and informative among our studied populations. Genetic homogeneities were revealed between the modern populations on the Shandong Peninsula and Han nationalities from Northeastern China as well as East China.
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http://dx.doi.org/10.1080/03014460.2021.1879264DOI Listing
March 2021

Electronic modulation of nickel selenide by copper doping and carbon coating towards high-rate and high-energy density lithium ion half/full batteries.

Nanoscale 2020 Dec;12(46):23645-23652

Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, 215123, China.

Over the past decades, metal selenides have drawn considerable attention due to their high theoretical specific capacity. However, huge volume changes and sluggish electrochemical transfer kinetics hinder their applications in energy storage and conversion. In this work, we demonstrate an efficient and ingenious synthesis strategy to regulate nickel selenide electrodes by the introduction of copper and in situ coating with carbon ([email protected]). When used as anodes for lithium-ion batteries, the as-synthesized [email protected] delivered a high capacity of 1630 mA h g-1 at 1.0 A g-1 after 200 cycles and excellent rate performance as well as long-term cycling stability with a high capacity of 489 mA h g-1 at 10 A g-1 after 20 000 cycles. When coupled with a commercial LiFePO4 cathode, the full cells showed a high capacity of 463 mA h g-1 at 0.2 A g-1. Their superior electrochemical performance can be attributed to the synergistic effect of the in situ carbon coating and copper doping, which can promote the electron/ion transfer kinetics, as well as alleviate the volume expansion during cycling. This work will open new opportunities for the development of high-performance anodes for lithium storage.
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http://dx.doi.org/10.1039/d0nr06614aDOI Listing
December 2020

Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

BMC Plant Biol 2020 Nov 9;20(1):511. Epub 2020 Nov 9.

State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Key Laboratory of Spring Rapeseed Genetic Improvement, Spring Rapeseed Research and Development Center of Qinghai Province, Qinghai Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, 810016, Qinghai, China.

Background: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus, there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits.

Results: The cqSPDA2 locus was mapped to an interval of 21.87-22.91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2, we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BCF population were analyzed using these indel primers. Among these indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval, and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BCF population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis.

Conclusions: The locus cqSPDA2, a dominant QTL for chlorophyll content in Brassica napus, was fine-mapped to a 21.89-22.04 Mb interval on A02 Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) that may be responsible for chlorophyll synthesis were found.
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http://dx.doi.org/10.1186/s12870-020-02710-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654151PMC
November 2020

One-dimensional nitrogen-doped carbon frameworks embedded with zinc-cobalt nanoparticles for efficient overall water splitting.

J Colloid Interface Sci 2021 Mar 20;585:800-807. Epub 2020 Oct 20.

Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, PR China. Electronic address:

Metal-organic frameworks (MOFs)-derived catalysts exhibit highly-efficient hydrogen or oxygen evolution performance on water splitting. However, it is an urgent problem to construct bifunctional electrocatalysts for both hydrogen and oxygen evolution performance. Herein, we adopted Ag nanowires as templates to prepare one-dimensional Ag [email protected]@ZIF-67 precursors (1D [email protected]@ZIF-67). Through pyrolysis, [email protected]@ZIF-67 precursors transformed into nitrogen-doped carbon frameworks (NCF) embedded with zinc-cobalt (ZnCo) nanoparticles on the surface of Ag NWs (denoted as [email protected]/NCF nanohybrids). The nanohybrids were consisted of Ag NWs with good conductivity and ZnCo/NCF nanohybrids with rich accessible active sites. Benefiting from their large specific surface area, accessible active sites and synergistic effect among components, [email protected]/NCF nanohybrids exhibit lower overpotentials of 139 mV and 279 mV at the current density of 10 mA cm for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) in alkaline solution, severally. Compared with other catalysts, [email protected]/NCF nanohybrids possess smaller Tafel slope, indicating their higher catalytic activity. This work provides a new perspective for designing low-cost and highly efficient bifunctional electrocatalysts for overall water splitting.
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http://dx.doi.org/10.1016/j.jcis.2020.10.060DOI Listing
March 2021

Visible-Light-Promoted Regioselective 1,3-Fluoroallylation of -Difluorocyclopropanes.

Org Lett 2020 Nov 28;22(21):8681-8686. Epub 2020 Oct 28.

Technical Institute of Fluorochemistry (TIF), Institute of Advanced Synthesis (IAS), School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.

A strategically novel protocol for ring-opening functionalization of aryl -difluorocyclopropanes (FCPs), which allows an expedient construction of CF-containing architectures via visible-light-promoted F-nucleophilic attack manifold, was disclosed. Single electron oxidation of FCPs was ascribed as the critical step for the success of this transformation by prompting F-nucleophilic attack, as well as the ensuing C-C bond scission. The observed intriguing regioselectivity for fluoroincorporation in this reaction was rationalized by invoking the cation-stabilization property of -difluorine substituents and also the thermodynamic gains acquired from forming CF functionality. By using cost-effective fluorination reagent and readily available substrates, a broad collection of structurally diversified α-allyl-β-trifluoromethyl ethylbenzene derivatives could be obtained in generally good yields. Further mechanistic investigations proved the engagement of a benzylic radical intermediate in this transformation.
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http://dx.doi.org/10.1021/acs.orglett.0c03268DOI Listing
November 2020

Feasibility of Chemically Modified Cellulose Nanofiber Membranes as Lithium-Ion Battery Separators.

ACS Appl Mater Interfaces 2020 Sep 2;12(37):41211-41222. Epub 2020 Sep 2.

Department of Chemical Engineering, KTH Royal Institute of Technology, Teknikringen 42, Stockholm SE-100 44, Sweden.

Chemical modification of cellulose is beneficial to produce highly porous lithium-ion battery (LIB) separators, but introduction of high charge density adversely affects its electrochemical stability in a LiNiMnCoO (NMC)/graphite full cell. In this study, the influence of carboxylate functional groups in 2,2,6,6-tetramethylpiperidine-1-oxyl-mediated oxidized cellulose nanofibers (TOCNs) on the electrochemical performances of the LIB separator was investigated. X-ray photoelectron spectroscopy and in operando mass spectrometry measurements were used to elucidate the cause of failure of the batteries containing TOCN separators in the presence and absence of sodium counterions in the carboxylate groups and additives. For the TOCN separator with sodium carboxylate functional groups, it seems that Na deposition is the dominant reason for poor electrochemical stability of the cell thereof. The poor performance of the protonated TOCN separator, attributed to a high amount of gas evolution, is dramatically improved by adding 2 wt % of vinylene carbonate (VC) because of suppressed gas evolution. Unveiling the failure mechanism of the TOCN separators and successively implementing the strategies to improve performance, for example, removing Na, adding VC, and adjusting cycling rates, enable a remarkable cycling performance in the NMC/graphite full cell at ≈2 C (3 mA/cm) of a fast discharging rate. Despite the aforementioned efforts and compromises required, an increased charge density of the TOCN is beneficial to acquire a mechanically stronger separator. In conclusion, the manufacturing process of cellulose nanofibers needs to be carefully adjusted to acquire a desired separator property. To the best of our knowledge, it is first reported to perform operando gas evolution measurements to systematically investigate the electrochemical stability of nanocellulose as an LIB separator material. The results elucidate not only the challenges for extensive applications of hygroscopic biomaterials for commercial LIBs but also the practical solutions to achieve high electrochemical stability of the materials.
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http://dx.doi.org/10.1021/acsami.0c08820DOI Listing
September 2020

Association between serum Cystatin C and renal injury in patients with chronic hepatitis B.

Medicine (Baltimore) 2020 Aug;99(32):e21551

Department of Hepatology, Qingdao Sixth People's Hospital, Qingdao, China.

To explore the association between serum cystatin C (Cys-C) and renal damage in patients with chronic hepatitis B.We retrospectively analyzed the clinical data of 425 patients with chronic hepatitis B virus (HBV) infection. Liver stiffness measured by FibroScan was used to diagnosis liver fibrosis. Cys-C levels were detected via latex-enhanced immunoturbidimetric assay.A total of 425 patients were enrolled. Among them, 217 were patients with CHB with an eGFR > 90 mL/min/1.73 m and 208 with an eGFR ≤90 mL/min/1.73 m. Cys-C levels significantly differed in patients with eGFR > 90 mL/min/1.73 m compared with patients with eGFR ≤90 mL/min/1.73 m (0.81 ± 0.05 vs 1.05 ± 0.06 mg/L, P < .001). Moreover, the Cys-C levels were 0.82 ± 0.04 mg/L in patients without liver fibrosis, 0.98 ± 0.05 mg/L in patients with mild liver fibrosis, 1.05 ± 0.08 mg/L in patients with advanced liver fibrosis, and 1.12 ± 0.07 mg/L in patients with liver cirrhosis (P < .001). Multivariate analyses were conducted to explore the independent factors associated with a decreased eGFR. Multivariate analysis suggested that T2DM (P = .032), liver fibrosis (P = .013), and Cys-C level (P = .035) were the independent factors associated with the decreased eGFR in patients with CHB. While age (P = .020) and Cys-C level (P = .001) were the independent factors associated with the decreased eGFR in patients with CHB-related fibrosis.The fibrosis group had significantly higher Cys-C levels than those without fibrosis. Routine monitoring of Cys-C levels is of positive significance in preventing the development of renal impairment of CHB patients.
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http://dx.doi.org/10.1097/MD.0000000000021551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593051PMC
August 2020

Updated population genetic data of 15 autosomal STR loci in a Shandong Han population from East China and genetic relationships among 26 Chinese populations.

Ann Hum Biol 2020 Aug 28;47(5):472-477. Epub 2020 Apr 28.

Center of Forensic Expertise, Affiliated hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Background: The population genetics investigation of STR loci in specific populations is the basic premise for forensic practice, and the AmpFlSTRTM IdentifilerTM Plus kit is still widely used in most forensic DNA laboratories.

Aim: To obtain more reliable and accurate population genetic data of 15 autosomal STRs in the Shandong Han population and explore the genetic relationship with 25 neighbouring Chinese populations.

Subjects And Methods: A total of 5356 unrelated Chinese Han individuals from Shandong Province were genotyped using the IdentifilerTM Plus Kit. Allele frequencies and corresponding forensic statistical parameters were calculated. Population comparisons were further explored through the neighbour-joining tree and multidimensional scaling analysis.

Results: A total of 217 alleles were calculated, the allele frequencies spanned from 0.0001 to 0.5340. The combined discrimination power (CDP) and the combined probability of paternity (CPE) of 15 STR loci were 0.99999999999999998755 and 0.999994524, respectively. Population comparisons demonstrated that Shandong Han have genetic homogeneity with most Sino-Tibetan populations.

Conclusions: Our study updates the Shandong Han population database with a large sample size. These 15 STR loci in the Shandong Han population are more polymorphic and discriminatory than shown in previous data, which could be more applicable to personal identification and paternity testing, as well other population genetics studies.
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http://dx.doi.org/10.1080/03014460.2020.1749928DOI Listing
August 2020

Overexpression of long non-coding RNA cancer susceptibility 11 is involved in the development of chemoresistance to carboplatin in hepatocellular carcinoma.

Oncol Lett 2020 Mar 8;19(3):1993-1998. Epub 2020 Jan 8.

Department of Hepatology, The Sixth People's Hospital of Qingdao, Qingdao, Shandong 266033, P.R. China.

The long non-coding (lnc)RNA cancer susceptibility 11 (CASC11) promotes gastric cancer, however its role in other diseases is unknown. The present study demonstrated upregulation of lncRNA CASC11 and microRNA (miR)-21 in hepatocellular carcinoma (HCC). Furthermore, the expression of CASC11 was positively correlated with that of miR-21 in HCC tumors. Moreover, overexpression of lncRNA CASC11 led to upregulation of miR-21 in HCC cells, whereas overexpression of miR-21 had no effect on CASC11 levels. The levels of lncRNA CASC11 and miR-21 were found to be upregulated in the plasma of patients with HCC during chemotherapy. cell experiments demonstrated upregulation of lncRNA CASC11 in HCC cells treated with carboplatin. Additionally, overexpression of lncRNA CASC11 promoted, whereas its knockdown inhibited the viability of HCC cells following carboplatin treatment. Finally, overexpression of miR-21 ameliorated the effects of lncRNA CASC11 knockdown on cell viability. Thus, these findings suggest that upregulation of lncRNA CASC11 is involved in the development of chemoresistance to carboplatin in patients with HCC, via the upregulation of miR-21.
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http://dx.doi.org/10.3892/ol.2020.11265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039114PMC
March 2020

Interchangeability of two Enterovirus 71 inactivated vaccines in Chinese children: A phase IV, open-label, and randomized controlled trial.

Vaccine 2020 03 14;38(12):2671-2677. Epub 2020 Feb 14.

Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, China; Academy of Preventive Medicine, Shandong University, Jinan, China. Electronic address:

Background: In China, three inactivated Enterovirus 71 (EV71) vaccines have been approved. Although the vaccines in an immunization series should be from a single manufacture, children sometimes have to receive EV71 vaccines from more than one manufacturers. The aim of this study was to evaluate the interchangeability and safety of vaccination with EV71 vaccines from two manufacturers among Chinese children.

Methods: We conducted an open label and randomized controlled study among children aged 6-35 months from November 2018 to January 2019. The participants were randomly assigned (1:1:1:1) to receive EV71 vaccines in one of the four different schedules (two using a single vaccine for all doses from one manufacture, and two mixed schedules using vaccines from two manufactures). Blood samples were collected pre-vaccination (Day 0) and one month after the second dose (Day 60) for neutralizing antibody assay. Immunogenicity was assessed in the per-protocol cohort and safety was assessed in the total vaccinated cohort.

Results: A total of 300 children were enrolled and randomized, of whom 89.0% (267/300) were included in the per-protocol cohort for immunogenicity analysis. The seroconversion rates of the EV71 neutralizing antibody in four groups ranged from 98.4% to 100.0%, and were not significantly different among the groups. Compared with other groups, geometric mean titer was higher in group D, in which the participants received Institute of Medical Biology Chinese Academy of Medical Sciences (CAMS) vaccine in the first dose and the Sinovac vaccine in the second dose. Safety profiles were similar among the four groups and no serious adverse events related to the vaccination were reported.

Conclusions: Interchangeability of EV71 vaccines from two manufactures to complete an immunization series showed good immunogenicity and safety. The antibody response levels may vary by vaccination sequences of EV71 vaccines from the two manufacturers.

Trial Registration: ClinicalTrials.govNCT03873740.
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http://dx.doi.org/10.1016/j.vaccine.2020.02.013DOI Listing
March 2020

Ginger essential oil-based microencapsulation as an efficient delivery system for the improvement of Jujube (Ziziphus jujuba Mill.) fruit quality.

Food Chem 2020 Feb 4;306:125628. Epub 2019 Oct 4.

Tianjin Gasin-DH Preservation Technology Co., Ltd, Tianjin 300300, China.

Microencapsulation of Zingiber officinale essential oil (EO) in polysaccharide, chitosan (CH) and sodium carboxymethyl cellulose (CMC) based on the electrostatic interaction between charged polysaccharides at pH 3.0 in dual delivery system. Ratio variations of CH and CMC in microencapsulation were studied at 1:2, 2:1 and 1:1. This study aimed to evaluate the influence of the encapsulating materials combination on freeze-dried EO powders and to present the mechanisms for loading and releasing EO involved in the preparation of CH/CMC microcapsules. The spectroscopy analysis, physical properties, microstructural, encapsulation efficiency and EO release behavior in obtained EO microparticles were evaluated by using the analysis of fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and gas chromatography mass spectrometry (GC-MS), respectively. Afterwards, the above prepared microcapsules were applied on winter jujube fruit (Ziziphus jujuba Mill.) preservation. Results demonstrated that both the microstructure and stability of microencapsulation were improved in delivery system loading with CH and CMC (1:1) with the encapsulation efficiency of 88.50%, compared to other ratios of CH and CMC (1:2 and 2:1). Furthermore, the microencapsulation had a capacity to control and reduce the EO release, therefore the morphological and sensory quality of jujube fruits in EO delivery system during storage was enhanced significantly (P < 0.05), in comparison to control. Results revealed that the microparticles produced with CH and CMC (1:1) was considered to present better characteristics of microstructure, encapsulation efficiency, as well as to maintain higher nutritional quality for jujube fruit. Thus, EO microencapsulation loaded in CH/CMC-based dual delivery system has potential application and developmental value prospects in food industries.
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http://dx.doi.org/10.1016/j.foodchem.2019.125628DOI Listing
February 2020

Efficient BiVO Photoanodes by Postsynthetic Treatment: Remarkable Improvements in Photoelectrochemical Performance from Facile Borate Modification.

Angew Chem Int Ed Engl 2019 Dec 8;58(52):19027-19033. Epub 2019 Nov 8.

Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 10044, Stockholm, Sweden.

Water-splitting photoanodes based on semiconductor materials typically require a dopant in the structure and co-catalysts on the surface to overcome the problems of charge recombination and high catalytic barrier. Unlike these conventional strategies, a simple treatment is reported that involves soaking a sample of pristine BiVO in a borate buffer solution. This modifies the catalytic local environment of BiVO by the introduction of a borate moiety at the molecular level. The self-anchored borate plays the role of a passivator in reducing the surface charge recombination as well as that of a ligand in modifying the catalytic site to facilitate faster water oxidation. The modified BiVO photoanode, without typical doping or catalyst modification, achieved a photocurrent density of 3.5 mA cm at 1.23 V and a cathodically shifted onset potential of 250 mV. This work provides an extremely simple method to improve the intrinsic photoelectrochemical performance of BiVO photoanodes.
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http://dx.doi.org/10.1002/anie.201911303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6973097PMC
December 2019

Selective C-F bond carboxylation of -difluoroalkenes with CO by photoredox/palladium dual catalysis.

Chem Sci 2019 Jul 28;10(27):6721-6726. Epub 2019 May 28.

Institute of Advanced Synthesis , School of Chemistry and Molecular Engineering , Nanjing Tech University , Nanjing 211816 , P. R. China . Email:

The catalytic C-F bond carboxylation of organofluorines with CO gas remains a challenging problem in synthetic chemistry. Here, we describe a selective defluorinative carboxylation of -difluoroalkenes through photoredox/palladium dual catalysis. The C-F bond activation is enabled by single electron reduction through photoredox catalysis to generate a fluorovinyl radical, which subsequently participates in an unprecedented palladium-catalyzed carboxylation. This novel C-F functionalization proved applicable to a wide range of substituted -difluoroalkenes, providing a rapid access to valuable α-fluoroacrylic acids.
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http://dx.doi.org/10.1039/c9sc01336aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625485PMC
July 2019

Photoredox-Coupled F-Nucleophilic Addition: Allylation of gem-Difluoroalkenes.

Angew Chem Int Ed Engl 2019 Mar 13;58(12):3918-3922. Epub 2019 Feb 13.

Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, Jiangsu, 210009, P. R. China.

A novel strategy for the expedient construction of CF -embeded tertiary/quarternary carbon centers was developed by taking advantage of photoredox catalysis. Thanks to a key step of single-electron oxidation, electron-rich gem-difluoroalkenes, which otherwise are essentially reluctant towards F-nucleoplilic addition, now readily participate in this fluoroallylation reaction. Furthermore, this strategy provides an elegant example for the generation, as well as functionalization, of α-CF -substituted benzylic radical intermediates using cheap and readily available starting materials.
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http://dx.doi.org/10.1002/anie.201814308DOI Listing
March 2019

Association between NF-κB expression and drug resistance of liver cancer.

Oncol Lett 2019 Jan 30;17(1):1030-1034. Epub 2018 Oct 30.

Department of Digestive Diseases, The Sixth People's Hospital of Qingdao, Qingdao, Shandong 266033, P.R. China.

Association between the expression of nuclear factor κB (NF-κB) and the drug resistance of hepatoma cells was investigated. HepG-2 cells and HepG2/ADM cells were cultured, respectively. The morphology and status of the two groups of cells were observed by cell white light. The immunofluorescence by NF-κB and MDR1 staining on HepG-2 cells and HepG2/ADM cells, respectively, was applied and the fluorescence expression in the two groups of cells was observed. RT-qPCR was used to detect the expression of NF-κB and MDR1 mRNA, the NF-κB and MDR1 protein expression was detected by western blot analysis. The results of cell white illumination showed that the structure of HepG-2 and HepG2/ADM cells was complete and the cell morphology was normal, and there was no significant difference, and could be used for comparative study. Immunofluorescence staining showed that the expression of NF-κB and MDR1 in HepG-2 cells was very low, while the expression of NF-κB and MDR1 in HepG2/ADM cells was increased significantly. The RT-qPCR results showed that NF-κB and MDR1 mRNA expression in HepG-2 cells was very low, while NF-κB and MDR1 mRNA expression in HepG-2/ADM cells was significantly increased, and western blot results showed that NF-κB and MDR1 protein expression in HepG-2 cells was very low, while NF-κB and MDR1 protein expression in HepG-2/ADM cells was increased significantly. The results of variance analysis showed that there was significant difference in the expression of the control group and paeonol group (P<0.01). In conclusion, the expression of NF-κB in the drug-resistant cells of liver cancer is closely related to the resistance-related gene . This result may provide a new solution for the drug resistance of liver cancer.
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http://dx.doi.org/10.3892/ol.2018.9640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312998PMC
January 2019

Astragaloside IV combating liver cirrhosis through the PI3K/Akt/mTOR signaling pathway.

Exp Ther Med 2019 Jan 13;17(1):393-397. Epub 2018 Nov 13.

Department of Hepatology, The Sixth People's Hospital of Qingdao, Qingdao, Shandong 266033, P.R. China.

Astragaloside IV (AS-IV) in improving liver cirrhosis injury in rats and its effect on the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mechanistic target of rapamycin (mTOR) signaling pathway were observed. Rat model of liver cirrhosis was induced by injection of carbon tetrachloride (CCl). A total of 36 Sprague-Dawley (SD) rats were randomly divided into three groups: the normal control group (n=10), the model control group (n=13), and the AS-IV group (n=13). The normal control group was injected with olive oil and given carboxymethyl cellulose (CMC)-Na (10 ml/kg/day), the model control group was given CMC-Na (10 ml/kg/day), and the AS-IV group underwent intragastric administration of AS-IV (20 ml/kg/day). The content of alanine transaminase (ALT) and aspartate transaminase (AST) of rats was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-1β in serum were detected via enzyme-linked immunosorbent assay (ELISA). Hematoxylin and eosin (H&E) staining was applied to observe morphological changes in liver tissues. The expression of collagens in liver tissues was detected via Masson's trichrome staining. Additionally, the expression of proteins in liver tissues was detected via western blotting. Compared with those in the blank group, the levels of AST, ALT, TNF-α, IL-6 and IL-1β were higher, the expression level of collagens in liver tissues was increased, and the expression ratios of phosphorylated (p)-PI3K/PI3K, p-Akt/Akt and p-mTOR/mTOR proteins were increased in the model group. Compared with the model group, AS-IV could significantly decrease the content of AST, ALT, TNF-α, IL-6 and IL-β in serum of rats, obviously inhibit the expression of collagens in liver tissues and decrease the expression ratios of p-PI3K/PI3K, p-Akt/Akt and p-mTOR/mTOR proteins in liver tissues. AS-IV can inhibit the inflammatory response so as to reduce the expression of collagens, and its mechanism may play a key role by inhibiting the PI3K/Akt/mTOR signaling pathway.
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http://dx.doi.org/10.3892/etm.2018.6966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307369PMC
January 2019

Analysis of serum alpha-fetoprotein (AFP) and AFP-L3 levels by protein microarray.

J Int Med Res 2018 Oct 15;46(10):4297-4305. Epub 2018 Aug 15.

1 Department of General Surgery, Beijing YouAn Hospital, Capital Medical University, Beijing, China.

Objectives This study aimed to examine a simple, effective, time-saving, and low-cost protein microarray method for detecting serum alpha-fetoprotein (AFP) and AFP-L3 levels. Methods Serum samples from patients with hepatocellular carcinoma (HCC) (n = 33) and control subjects (n = 39) were collected and evaluated for the presence of AFP using a novel protein microarray. Glycoprotein (including AFP-L3) was enriched from crude samples by a Hotgen Biotech glycosyl capture spin column and then detected by protein microarray. An electrochemiluminescence immunoassay (ECLIA) was used to validate the measured values. Results Neither AFP levels lower than 20 ng/mL in the HCC group nor AFP levels higher than 20 ng/mL in the control group were found when tested by the ECLIA and protein microarray. The kappa test showed good consistency in the diagnostic performance of measuring serum AFP levels and the percentage of AFP-L3 in total AFP by the ECLIA and protein microarray. Protein microarray had advantages of smaller sample size required, low cost, and convenience compared with the ECLIA. Conclusion The protein microarray assay that was developed in the present study shows potential as an economic and convenient technique for detecting AFP and AFP-L3 levels in serum samples from patients with HCC.
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http://dx.doi.org/10.1177/0300060518789304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166357PMC
October 2018

Intermolecular Carboamination of Unactivated Alkenes.

J Am Chem Soc 2018 08 16;140(34):10695-10699. Epub 2018 Aug 16.

Institute of Advanced Synthesis, College of Chemistry and Molecular Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials , Nanjing Tech University , Nanjing , Jiangsu 210009 , People's Republic of China.

Herein, we report the first example of group transfer radical addition of O-vinylhydroxylamine derivatives onto unactivated alkenes. By utilizing O-vinylhydroxylamine derivatives as both the N- and C-donors, this reaction enables intermolecular carboamination of unactivated alkenes in an atom economical fashion. As the process is initiated through N-radical addition followed by C-transfer, linear carboamination products are afforded. This differs from canonical radical carbofunctionalization of olefins, which typically favors branched product owing to initiation by C-radical addition.
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http://dx.doi.org/10.1021/jacs.8b07023DOI Listing
August 2018

Tenofovir decrease hepatocellular carcinoma recurrence in chronic hepatitis B patients after liver resection.

Infect Agent Cancer 2018 8;13:19. Epub 2018 Jun 8.

Invasive Technology Department, Jining NO.1 People's Hospital, Jining, Shandong 272100 People's Republic of China.

Background: Tenofovir disoproxil fumarate (TDF) and entecavir (ETV) are recommended as the first-line choices regarding the treatment of chronic hepatits B. The impact of the two antiviral agents on prognosis of Chronic hepatitis B (CHB) related hepatocellular carcinoma (HCC) remains to be explored. We aim to investigate whether CHB-related HCC patients receiving TDF and ETV have a different prognosis.

Methods: 233 CHB-related compensated cirrhosis patients were divided into groups according to the nucleut(s)ide patients received. The results of TDF and ETV groups were reviewed and compared. The disease-free survival (DFS) and overall survival (OS) of both groups were analyzed and compared.

Results: 233 CHB-related compensated cirrhosis patients from 2013 October to 2014 November were included in our study. 107 and 126 patients received TDF and ETV monotherapy, respectively. Child-Pugh score, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin level, status of hepatitis B e antigen (HBeAg) and serum HBV DNA level were compared between groups. DFS in TDF-treatment group were significantly longer than it in ETV-treatment group ( < 0.05). multi-variant analysis indicated that TDF duration was significantly associated with lower probability of HCC development, (hazard ratio, 0.35; 95% confidence interval range, 0.33-0.84,  < 0.05).

Conclusion: Anti-virus regimen containing TDF benefits for the prognosis of CHB-related liver cirrhosis patients.
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http://dx.doi.org/10.1186/s13027-018-0191-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994044PMC
June 2018

Unified equivalent circuit model for carbon nanotube-based nanocomposites.

Nanotechnology 2018 Jul 9;29(30):305503. Epub 2018 May 9.

School of Manufacturing Science and Engineering, Key Laboratory of Testing for Manufacturing Process, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, People's Republic of China.

Carbon nanotubes form a complex network in nanocomposites. In the network, the configuration of the nanotubes is various. A carbon nanotube may be curled or straight, and it may be parallel or crossed to another. As a result, carbon nanotube-based composites exhibit integrated characteristics of inductor, capacitor and resistor. In this work, it is hypothesised that carbon nanotube-based composites all adhere to a RLC interior circuit. To verify the hypothesis, three different composites, viz multi-walled carbon nanotube/polyvinylidene fluoride (MWCNT/PVDF), multi-walled carbon nanotube/epoxy (MWCNT/EP), multi-walled carbon nanotube/polydimethylsiloxane (MWCNT/PDMS) were fabricated and tested. The resistances and the dielectric loss tangent (tanδ) of the materials were measured in direct and alternating currents. The measurement shows that the value of tanδ is highly affected by the volume fraction of MWCNT in the composites. The experimental results prove that the proposed RLC equivalent circuit model can fully describe the electrical properties of the MWCNT network in nanocomposites. The RLC model provides a new route to detect the inductance and capacitance of carbon nanotubes. Moreover, the model also indicates that the carbon nanotube-based composite films may be used to develop wireless strain sensors.
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http://dx.doi.org/10.1088/1361-6528/aac36dDOI Listing
July 2018

On some nonlinear retarded Volterra-Fredholm type integral inequalities on time scales and their applications.

Authors:
Haidong Liu

J Inequal Appl 2018 15;2018(1):211. Epub 2018 Aug 15.

School of Mathematical Sciences, Qufu Normal University, Qufu, P.R. China.

In this paper, we establish some new nonlinear retarded Volterra-Fredholm type integral inequalities on time scales. Our results not only generalize and extend some known integral inequalities, but also provide a handy and effective tool for the study of qualitative properties of solutions of some Volterra-Fredholm type dynamic equations.
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http://dx.doi.org/10.1186/s13660-018-1808-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096914PMC
August 2018

An improvement of the Lyapunov inequality for certain higher order differential equations.

Authors:
Haidong Liu

J Inequal Appl 2018 17;2018(1):215. Epub 2018 Aug 17.

School of Mathematical Sciences, Qufu Normal University, Qufu, P.R. China.

In this paper, we establish two Lyapunov inequalities for some half-linear higher order differential equations with anti-periodic boundary conditions. Our result improves that obtained by Wang (Appl. Math. Lett. 25:2375-2380, 2012).
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http://dx.doi.org/10.1186/s13660-018-1809-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105191PMC
August 2018

Ar-P bond construction by the Pd-catalyzed oxidative cross-coupling of arylsilanes with H-phosphonates via C-Si bond cleavage.

Chem Commun (Camb) 2017 Jan;53(5):956-958

Department of Chemistry & Chemical Engineering, Gannan Normal University, Ganzhou 341000, China.

A novel and efficient methodology that allows for the construction of Ar-P bonds via the Pd-catalyzed oxidative cross-coupling reaction of various arylsilanes with H-phosphonates leading to valuable arylphosphonates has been developed.
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http://dx.doi.org/10.1039/c6cc08408gDOI Listing
January 2017

IL-22-induced miR-122-5p promotes keratinocyte proliferation by targeting Sprouty2.

Exp Dermatol 2017 04;26(4):368-374

Department of Dermatology, Qilu Hospital, Shangdong University, Jinan, Shandong, China.

Psoriasis is a common inflammatory skin disease, but the exact pathogenesis is largely unknown. Interleukin-22 (IL-22) has demonstrated its vital role in T-cell-mediated immune response by interacting with keratinocytes in the pathogenesis of psoriasis. Here, we showed the differentially expressed miRNAs and their potential targets in HaCaT cells stimulated by IL-22 using miRNA and mRNA microarrays. We revealed a total of 20 significantly changed (more than twofold) miRNAs in HaCaT cells and validated the results with quantitative reverse transcriptase PCR (qRT-PCR). We demonstrated that miR-122-5p was up-regulated both in HaCaT cells stimulated by IL-22 and in psoriatic lesions. Then, we aimed to investigate the biological roles and potential mechanism of miR-122-5p in keratinocytes. As a result, CCK-8 assay indicated that overexpression of miR-122-5p in keratinocytes promoted proliferation and conversely inhibition of endogenous miR-122-5p suppressed proliferation. According to the microarray analysis, we assumed that Sprouty2 (Spry2), a negative regulator of extracellular signal regulated kinase/mitogen-activated protein kinase signalling pathway, was a direct target gene of miR-122-5p. We found that the staining of Spry2 in cytoplasm was mainly localized in both basal and suprabasal layers of epidermis and showed a markedly decreased expression in psoriasis than in normal control by immunohistochemistry. Luciferase reporter and Western blot assays in HaCaT cells demonstrated that Spry2 was a direct target gene of miR-122-5p. In conclusion, IL-22-induced miR-122-5p promotes keratinocyte proliferation possibly by downregulating the expression of Spry2 thus playing important roles in the pathogenesis of psoriasis.
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http://dx.doi.org/10.1111/exd.13270DOI Listing
April 2017
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