Publications by authors named "Haibing Liu"

14 Publications

  • Page 1 of 1

Effects of long-distance transportation on blood constituents and composition of the nasal microbiota in healthy donkeys.

BMC Vet Res 2020 Sep 15;16(1):338. Epub 2020 Sep 15.

College of Veterinary Medicine, Yangzhou University, 225009, Yangzhou, P.R. China.

Background: This study aims to determine the effects of transportation on the nasal microbiota of healthy donkeys using 16S rRNA sequencing.

Results: Deep nasal swabs and blood were sampled from 14 donkeys before and after 21 hours' long-distance transportation. The values of the plasma hormone (cortisol (Cor), adrenocorticotrophic hormone (ACTH)), biochemical indicators (total protein (TP), albumin (ALB), creatinine (CREA), lactic dehydrogenase (LDH), aspartate transaminase (AST), creatine kinase (CK), blood urea (UREA), plasma glucose (GLU)) and blood routine indices (white blood cell (WBC), lymphocyte (LYM), neutrophil (NEU), red blood cell (RBC), hemoglobin (HGB)) were measured. 16S rRNA sequencing was used to assess the nasal microbiota, including alpha diversity, beta diversity, and phylogenetic structures. Results showed that levels of Cor, ACTH, and heat-shock protein 90 (HSP90) were significantly increased (p < 0.05) after long-distance transportation. Several biochemical indicators (AST, CK) and blood routine indices (Neu, RBC, and HGB) increased markedly (p < 0.05), but the LYM decreased significantly (p < 0.05). Nine families and eight genera had a mean relative abundance over 1%. The predominant phyla in nasal microbiota after and before transportation were Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Transportation stress induced significant changes in terms of nasal microbiota structure compared with those before transportation based on principal coordinate analysis (PCoA) coupled with analysis of similarities (ANOSIM) (p < 0.05). Among these changes, a notably gain in Proteobacteria and loss in Firmicutes at the phylum level was observed.

Conclusions: These results suggest transportation can cause stress to donkeys and change the richness and diversity of nasal microbiota. Further studies are required to understand the potential effect of these microbiota changes on the development of donkey respiratory diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12917-020-02563-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493398PMC
September 2020

Enhancing Privacy and Data Security across Healthcare Applications Using Blockchain and Distributed Ledger Concepts.

Healthcare (Basel) 2020 Jul 29;8(3). Epub 2020 Jul 29.

Computer Science Department, School of Engineering and Technology, Universidad Internacional de la Rioja (UNIR), 26006 Logroño, Spain.

Nowadays, blockchain is developing as a secure and trustworthy platform for secure information sharing in areas of application like banking, supply chain management, food industry, energy, the Internet, and medical services. Besides, the blockchain can be described in a decentralized manner as an immutable ledger for recording data entries. Furthermore, this new technology has been developed to interrupt a variety of data-driven fields, including the health sector. However, blockchain refers to the distributed ledger technology, which constitutes an innovation in the information recording and sharing without a trusted third party. In this paper, blockchain and Distributed Ledger-based Improved Biomedical Security system (BDL-IBS) has been proposed to enhance the privacy and data security across healthcare applications. Further, our goal is to make it possible for patients to use the data to support their care and to provide strong consent systems for sharing data among different organizations and applications, since this includes managing and accessing a high amount of medical information, and this technology can maintain data to ensure reliability. Finally, results show that new blockchain-based digital platforms allow for fast, easy, and seamless interactions between data suppliers to enhance privacy and data security, including for patients themselves.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/healthcare8030243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7684467PMC
July 2020

Computational analysis and verification of molecular genetic targets for glioblastoma.

Biosci Rep 2020 06;40(6)

Department of Neurosurgery, 900 Hospital of the Joint Logistics Team, Fuzhou, Fujian 350025, P.R. China.

Background: Glioblastoma (GBM) is the most common malignant brain tumor with a poor prognosis. The initial treatment for high-grade gliomas is surgical excision. However, even with concomitant use of radiation or chemotherapy, patients are still prone to recurrence. The specific pathogenesis of GBM is still controversial.

Methods: Differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) between GBM and normal brain tissues were screened. P-value was obtained by Bayes test based on the limma package. Statistical significance was set as P-value <0.05 and |Fold change (FC)| > 0.2 (GSE90886); P-value <0.05 and |FC| > 1 (GSE116520, GSE103228). Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG), protein-protein interaction (PPI) network were performed. Hub genes were selected from miRNA target genes and DEGs. GBM and normal brain tissues were extracted to verify the expression.

Results: A total of 100 DEGs were overlapped in both datasets. Analysis of pathways and process enrichment tests indicated that ion transport, positive regulation of macromolecule metabolic process, cell cycle, axon guidance were enriched in the GBM. Sixteen hub genes were identified. Hub genes ADARB1 and neuropilin 1 (NRP1) were significantly associated with overall survival (OS) and disease-free survival (DFS) (P<0.05). Eukaryotic translation termination factor 1 (ETF1) was associated with DFS (P<0.05).

Conclusions: DEGs and DEMs were found between GBM tumor tissues and normal brain tissues. These biomarkers may be used as targets for early diagnosis and specific treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1042/BSR20201401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298167PMC
June 2020

Anterior communicating artery complex fenestration combined with tandem aneurysm: a case report and literature review.

Medicine (Baltimore) 2020 May;99(19):e20013

Department of Neurosurgery, Fuzong Clinical Medical College of Fujian Medical University, 900th Hospital.

Introduction: Clinically, anterior communicating artery complex fenestration combined with fenestration-related aneurysms is rare, and combination of this condition with tandem aneurysms is even rarer.

Patient Concerns: A case of a 43-year-old man with spontaneous subarachnoid hemorrhage.

Diagnosis: A computed tomography angiography examination revealed a fenestrated anterior communicating artery complex combined with 2 aneurysms. Then, a digital subtraction angiography examination was performed to further determine the diagnosis, which showed a complex anatomical structure of the local tissue. After the aneurysms ruptured, they were partially wrapped by a hematoma and compressed, which increased the difficulty of surgery.

Interventions: An endovascular interventional therapy method was chosen, and a simple coil was successfully inserted through the blood vessel into the tandem aneurysms to maintain the integrity of the anatomical structure.

Outcomes: The patient recovered well postoperatively. An imaging review after the operation did not show the aneurysms, and the upper and lower branches were patent.

Conclusion: Therefore, endovascular treatment is an appropriate choice for arterial fenestration combined with tandem aneurysms, once the aneurysms have ruptured.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000020013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220171PMC
May 2020

[Associations of JAK1 gene polymorphisms with allergic rhinitis in Chinese Han populations].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2015 Oct;29(19):1713-6, 1722

Objective: The aim of this study was to investigate the association of JAK1 polymorphisms with allergic rhinitis in China Han population.

Method: A total of 450 patients with AR and 615 healthy subjects as control were genotyped for the presence of three single nucleotide polymorphisms using polymerase chain reaction restriction fragment length polymorphism (PGR-RFLP) analysis of DNA extracted from blood samples.

Result: All control subjects were in Hardy-Weinberg equilibrium, but high frequencies of JAK1 the homozygous rs310241 CC genotype were observed in AR patients compared to controls (P < 0.05). The results also revealed that there was no association between the rest of two investigated SNPs and AR.

Conclusion: Our results suggested that JAK1 gene rs310241 CC genotype was associated with patients with AR.
View Article and Find Full Text PDF

Download full-text PDF

Source
October 2015

Whole-Genome Sequencing for the Investigation of a Hospital Outbreak of MRSA in China.

PLoS One 2016 7;11(3):e0149844. Epub 2016 Mar 7.

Department of Clinical Laboratory, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.

Staphylococcus aureus is a globally disseminated drug-resistant bacterial species. It remains a leading cause of hospital-acquired infection, primarily among immunocompromised patients. In 2012, the Affiliated People's Hospital of Jiangsu University experienced a putative outbreak of methicillin-resistant S. aureus (MRSA) that affected 12 patients in the Neurosurgery Department. In this study, whole-genome sequencing (WGS) was used to gain insight into the epidemiology of the outbreak caused by MRSA, and traditional bacterial genotyping approaches were also applied to provide supportive evidence for WGS. We sequenced the DNA from 6 isolates associated with the outbreak. Phylogenetic analysis was constructed by comparing single-nucleotide polymorphisms (SNPs) in the core genome of 6 isolates in the present study and another 3 referenced isolates from GenBank. Of the 6 MRSA sequences in the current study, 5 belonged to the same group, clustering with T0131, while the other one clustered closely with TW20. All of the isolates were identified as ST239-SCCmecIII clones. Whole-genome analysis revealed that four of the outbreak isolates were more tightly clustered into a group and SA13002 together with SA13009 were distinct from the outbreak strains, which were considered non-outbreak strains. Based on the sequencing results, the antibiotic-resistance gene status (present or absent) was almost perfectly concordant with the results of phenotypic susceptibility testing. Various toxin genes were also analyzed successfully. Our analysis demonstrates that using traditional molecular methods and WGS can facilitate the identification of outbreaks and help to control nosocomial transmission.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0149844PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4780730PMC
July 2016

siRNA Targeting the 2Apro Genomic Region Prevents Enterovirus 71 Replication In Vitro.

PLoS One 2016 17;11(2):e0149470. Epub 2016 Feb 17.

Department of Clinical Laboratory, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.

Enterovirus 71 (EV71) is the most important etiological agent of hand, foot, and mouth disease (HFMD) in young children, which is associated with severe neurological complications and has caused significant mortalities in recent HFMD outbreaks in Asia. However, there is no effective antiviral therapy against EV71. In this study, RNA interference (RNAi) was used as an antiviral strategy to inhibit EV71 replication. Three small interfering RNAs (siRNAs) targeting the 2Apro region of the EV71 genome were designed and synthesized. All the siRNAs were transfected individually into rhabdomyosarcoma (RD) cells, which were then infected with strain EV71-2006-52-9. The cytopathic effects (CPEs) in the infected RD cells, cell viability, viral titer, and viral RNA and protein expression were examined to evaluate the specific viral inhibition by the siRNAs. The results of cytopathogenicity and MTT tests indicated that the RD cells transfected with the three siRNAs showed slight CPEs and significantly high viability. The 50% tissue culture infective dose (TCID50) values demonstrated that the viral titer of the groups treated with three siRNAs were lower than those of the control groups. qRT-PCR and western blotting revealed that the levels of viral RNA and protein in the RD cells treated with the three siRNAs were lower than those in the controls. When RD cells transfected with siRNAs were also infected with strain EV71-2008-43-16, the expression of the VP1 protein was significantly inhibited. The levels of interferon α (IFN-α) and IFN-β did not differ significantly in any group. These results suggest that siRNAs targeting the 2Apro region of the EV71 genome exerted antiviral effects in vitro.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0149470PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757562PMC
August 2016

[The expression and significance of VIP and its receptor in the cochlea of different degrees of chronic alcoholism rats].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2015 Jul;29(14):1295-8

Objective: To determine whether chronic alcoholism alters the expression levels of Vasoactive intestinal polypeptide (VIP) and its receptor (VIPR1) in the cochlea of chronic alcoholism rats.

Method: We measured their expression levels in 30 SD rats, in which we created models of different degrees of chronic alcoholism. We investigated the presence of the mRNA of VIP in the cochlea of chronic alcoholism rats and controls by reverse transcription-polymerase chain reaction (RT-PCR) method. We investigated the presence of proteins of VIPR1 in poisoned rats and controls by western blot. We also evaluated the local distribution of VIP cells by immunohistochemistry.

Result: We found that the levels of VIP and VIPR1 were downregulated in the chronic alcoholism groups compared to the controls group. The differences in some expression levels were significant different between chronic alcoholism rats and control rats. Moreover, at different degrees of alcohol poisoning in rats, the contents of VIP and VIPR1 differed. Decreased levels of VIP and VIPR1 were detected in the deep chronic alcoholism group compared to the group with low-degree poisoning (P < 0.05). In spiral ganglion cell plasm the expression of VIP and VIPR1 had no significant difference in three groups (P > 0.05).

Conclusion: These results suggest that VIP and VIPR1 play an important role in the auditory function in rats with chronic alcoholism. Chronic alcoholism may cause a peptide hormone secretion imbalance in the auditory system, eventually leading to hearing loss.
View Article and Find Full Text PDF

Download full-text PDF

Source
July 2015

[A case of the nasal septum pyogenic granuloma and literature].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2014 Oct;28(20):1614-6

A 56-year-old male had presented with left nasal bleeding repeatedly for 4 days. The pathological examination after resection showed pyogenic granuloma. Its etiology and pathogenesis, clinical features, pathological features and treatments were reviewed.
View Article and Find Full Text PDF

Download full-text PDF

Source
October 2014

Increased frequency of Th17 cells in the peripheral blood of children infected with enterovirus 71.

J Med Virol 2012 May;84(5):763-7

Department of Clinical Laboratory, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, China.

Enterovirus 71 (EV71) affects the health of young children globally causing severe neurologic diseases. The relationship between EV71 infection and T helper type 17 (Th17) has not been described, although this new Th subset or interleukin-17 (IL-17) has been reported to be associated with other viral infections. The purpose of the current study was to describe the immune profile involving Th17 cells, neutrophils, and related factors and to speculate on the possible immunopathogenesis of EV71 infections. Flow cytometry and an automatic blood cell counter were used to analyze circulating Th17 cells and count neutrophils, respectively. Expression of acid-related orphan nuclear receptor gamma t (ROR γt) was evaluated by reverse-transcriptional PCR, and enzyme linked immunosorbent assays (ELISAs) were used for detecting concentrations of IL-17, IL-23, and IFN-γ. The results showed that the frequencies of Th17 cells (1.47 ± 0.87%) and the number of neutrophils (7.4 ± 4.1 × 10(9) /L) in peripheral blood samples from children infected with EV71 were significantly higher compared to controls. In addition, there was a statistically higher expression of ROR γt in peripheral blood mononuclear cells (PBMCs) and elevated concentrations of IL-17 and IL-23 in sera, but lower IFN-γ production during EV71 infections. The findings suggest that Th17 cells are mediators during the immunologic process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jmv.23254DOI Listing
May 2012

[Diagnosis and treatment of laryngopharyngeal reflux disease].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2011 Aug;25(15):690-1

Department of Otolaryngology, the Central Hospital of Chengdu Railway, Chengdu, 610081, China.

Objective: To investigate the diagnosis and treatment of laryngopharyngeal reflux disease.

Method: Patients suspect of laryngopharyngeal reflux disease in our out-patient department from Jan 2008 to Jan 2009 were included in this study. All patients were assessed using the reflux symptom index(RSI) and the reflux finding score(RFS). The positive criteria of RSI was score > 13 and that of RFS was score > 7. Fifty-eight double positive patients accepted omeprazole oral administration. After continuous oral administration for three months, all patients received RSI and RFS assessment again.

Result: After treatment, the symptoms of 41 patients were significantly alleviated. In addition, RSI were positive in 41 cases and RFS were positive in 37 cases. Thirty-seven cases were double positive.

Conclusion: The RSI and RFS could serve as an effective assessment method and could accurately document treatment efficacy in patients with LPR. Proton pump inhibitor such as omeprazole is an effective treatment for patients with LPR.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2011

[Cholesteatoma of external auditory canal among 18 cases].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2007 Aug;21(16):746-7

Department of Otorhinolaryngology, Chengdu Railway Center Hospital, Chengdu, 610081, China.

Objective: To improve the diagnosis and treatment of the external auditory canal cholesteatoma (EACC).

Method: The data of 18 patients caused by EACC were analysed retrospectively.

Result: In all cases, the cholesteatomas were found in the external auditory canal and the mastoid cavity. CT scanning was using for definition the ranging of lesions and the timm management surgery was completed. There was no recurrence in 10-40 months follow-up.

Conclusion: EACC is easily misdiagnosed as keratosis obturans (KO). The disease can progress to extensive mastoid destruction. CT of temporal bone and detailed history is necessary in the diagnosis. Early complete surgical treatment is the best method.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2007

[Prognostic value of Ki67 and VEGF in squamous cell carcinoma of larynx].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2006 Mar;20(6):246-8

Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.

Objective: To investigate the prognostic value of Ki67 and VEGF in squamous cell carcinoma of larynx.

Method: The expressions of Ki67 and VEGF in squamous cell carcinoma of larynx tissues of 32 follow-up cases were studied by immunohistochemical staining, of which five cases recurred(recurrent group), three cases metastasized (metastatic group), eight case died(deceased group) and 24 cased survived(survival group) in three years after operations.

Result: The expressions of Ki67 and VEGF in deceased group were higher than those in survival group( P < 0.01). Overexpression of Ki67 could be found in recurrent group and in metastatic group (P < 0.01). And VEGF expressed higher in recurrent group than in nonrecurrent group (P < 0.05). On Cox regression of multivariate analysis, Ki67 (RR: 3.236, P < 0.01), clinical T stage (RR: 1.382, P < 0.05) and metastasis of lymph nodes (RR: 0.316, P < 0.05) were shown to be independent prognostic factors for survival of squamous cell carcinoma of larynx.

Conclusion: Ki67 and VEGF were related to the prognosis of squamous cell carcinoma of larynx, overexpression of the two markers indicated poor prognosis of the disease, and they might be helpful for the treatment of carcinoma of larynx.
View Article and Find Full Text PDF

Download full-text PDF

Source
March 2006

[The control study of glycerol test in different stage of Meniere's disease patients].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2005 Jun;19(12):543-4

Department of Otorhinolaryngology, Chengdu Railway Central Hospital, Chengdu, 610081, China.

Objective: To investigate the best time of the glycerol test on Meniere's disease patients.

Method: The glycerol test was carried out two times on 72 Meniere's disease patients, one time in the period of onset and the other in the period of intermission.

Result: The positive rate in the period of onset (83.3%) was far higher than in the period of intermission(43.1%), additionally, the positive rate is irrelevant to the degree of hearing impairing or the course of disease.

Conclusion: The glycerol test should be carried out in the period of onset as possible as you can to the suspicious patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
June 2005
-->