Publications by authors named "Haibin Wu"

102 Publications

Ceria Nanozyme-Integrated Microneedles Reshape the Perifollicular Microenvironment for Androgenetic Alopecia Treatment.

ACS Nano 2021 Jul 19. Epub 2021 Jul 19.

Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

Androgenetic alopecia (AGA) is highly prevalent in current society but lacks effective treatments. The dysregulation of the hair follicle niche induced by excessive reactive oxygen species (ROS) and insufficient vascularization in the perifollicular microenvironment is the leading cause of AGA. Herein, we designed a ceria nanozyme (CeNZ)-integrated microneedles patch (Ce-MNs) that can alleviate oxidative stress and promote angiogenesis simultaneously to reshape the perifollicular microenvironment for AGA treatment. On the basis of the excellent mechanical strength of Ce-MNs, the encapsulated CeNZs with catalase- and superoxide-mimic activities can be efficiently delivered into skin to scavenge excessive ROS. Moreover, the mechanical stimulation induced by the administration of MNs can remodel the microvasculature in the balding region. Compared with minoxidil, a widely used clinical drug for AGA treatment, Ce-MNs exhibited accelerated hair regeneration in the AGA mouse model at a lower administration frequency without inducing significant skin damage. Consequently, such a safe and perifollicular microenvironment-shaping MNs patch shows great potential for clinical AGA treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.1c05272DOI Listing
July 2021

The evolution of hydrochemical and isotopic signatures from precipitation, surface water to groundwater in a typical karst watershed, Central Texas, USA.

Isotopes Environ Health Stud 2021 Jul 16:1-24. Epub 2021 Jul 16.

Key Laboratory of Cenozoic Geology and Environment, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing, People's Republic of China.

The Upper Cibolo Creek (UCC) karst watershed in Central Texas, USA, represents a portion of the drainage area that supplies water to the recharge zone for the Edwards Aquifer. However, the surface water-groundwater interactions along the UCC are not well quantified, and the hydraulic interactions are important for water budget and water quality of the aquifer. In this study, we investigated the evolution of hydrochemical and isotopic signatures (O, H and -excess) from precipitation, surface water to groundwater in the UCC watershed from 2017 to 2019, and investigated surface water-groundwater interactions using samples from 14 creeks/spring sites. Factor analysis for the observed parameters demonstrates that changes in water hydrochemistry are primarily controlled by human activity, precipitation input, and water-rock interaction. Hierarchical clustering analysis of temporal isotope variations confirms that significant surface water-groundwater interactions occur in the UCC watershed. We identified relationships between nitrate concentrations at creek/spring sites and land-use conditions, and nitrate input sources were determined utilizing the dual-isotope analyses (N and O) of nitrate. This study provides capacity for a more precise assessment of water resources and water quality in Central Texas.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10256016.2021.1948410DOI Listing
July 2021

Establishment of a 3D model of tumor-driven angiogenesis to study the effects of anti-angiogenic drugs on pericyte recruitment.

Biomater Sci 2021 Jun 17. Epub 2021 Jun 17.

Laboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences, School of Medicine, South China University of Technology, Guangzhou 510006, P. R. China. and National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, P. R. China and Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, P. R. China and Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, P. R. China and Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, P. R. China.

Hepatocellular carcinoma (HCC), as a well-vascularized tumor, has attracted increasing attention in antiangiogenic therapies. Notably, emerging studies reveal that the long-term administration of antiangiogenic drugs induces hypoxia in tumors. Pericytes, which play a vital role in vascular stabilization and maturation, have been documented to be associated with antiangiogenic drug-induced tumor hypoxia. However, the role of antiangiogenic agents in regulating pericyte behavior still remains elusive. In this study, by using immunostaining analysis, we first demonstrated that tumors obtained from HCC patients were highly angiogenic, in which vessels were irregularly covered by pericytes. Therefore, we established a new 3D model of tumor-driven angiogenesis by culturing endothelial cells, pericytes, cancer stem cells (CSCs) and mesenchymal stem cells (MSCs) with microcarriers in order to investigate the effects and mechanisms exerted by antiangiogenic agents on pericyte recruitment during tumor angiogenesis. Interestingly, microcarriers, as supporting matrices, enhanced the interactions between tumor cells and the extracellular matrix (ECM), promoted malignancy of tumor cells and increased tumor angiogenesis within the 3D model, as determined by qRT-PCR and immunostaining. More importantly, we showed that zoledronic acid (ZA) reversed the inhibited pericyte recruitment, which was induced by sorafenib (Sora) treatment, through fostering the expression and activation of ErbB1/ErbB2 and PDGFR-β in pericytes, in both an in vitro 3D model and an in vivo xenograft HCC mouse model. Hence, our model provides a more pathophysiologically relevant platform for the assessment of therapeutic effects of antiangiogenic compounds and identification of novel pharmacological targets, which might efficiently improve the benefits of antiangiogenic treatment for HCC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0bm02107eDOI Listing
June 2021

Novel CD123 polyaptamer hydrogel edited by Cas9/sgRNA for AML-targeted therapy.

Drug Deliv 2021 Dec;28(1):1166-1178

Department of Neonatology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

CD123 targeting molecules have been widely applied in acute myelocytic leukemia (AML) therapeutics. Although antibodies have been more widely used as targeting molecules, aptamer have unique advantages for CD123 targeting therapy. In this study, we constructed an aptamer hydrogel termed as SSFH which could be precisely cut by Cas9/sgRNA for programmed SS30 release. To construct hydrogel, rolling-circle amplification (RCA) was used to generate hydrogel containing CD123 aptamer SS30 and sgRNA-targeting sequence. After incubation with Cas9/sgRNA, SSFH could lose its gel property and liberated the SS30 aptamer sequence, and released SS30 has been confirmed by gel electrophoresis. In addition, SS30 released from SSFH could inhibit cell proliferation and induce cell apoptosis . Moreover, SSFH could prolong survival rate and inhibit tumor growth via JAK2/STAT5 signaling pathway . Additionally, molecular imaging revealed SSFH co-injected with Cas9/sgRNA remained at the injection site longer than free aptamer. Furthermore, once the levels of cytokines were increasing, the complementary sequences of aptamers injection could neutralize SS30 and relieve side effect immediately. This study suggested that CD123 aptamer hydrogel SSFH and Cas9/sgRNA system has strong potential for CD123-positive AML anticancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10717544.2021.1934191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205012PMC
December 2021

Novel Compound Heterozygous Pathogenic Variants in Cause Isolated Sulfite Oxidase Deficiency in a Chinese Han Family.

Front Genet 2021 7;12:607085. Epub 2021 May 7.

Shaanxi Institute for Pediatric Diseases, Xi'an Children's Hospital, Xi'an, China.

Aim: To explore the clinical imaging, laboratory and genetic characteristics of a newborn boy with isolated sulfite oxidase deficiency (ISOD) in a Chinese mainland cohort.

Methods: Homocysteine and uric acid in plasma and cysteine and total homocysteine in the blood spot were assessed in a Chinese newborn patient with progressive encephalopathy, tonic seizures, abnormal muscle tone, and feeding difficulties. Whole exome sequencing and Sanger sequencing facilitated an accurate diagnosis. The pathogenicity predictions and conservation analysis of the identified mutations were conducted by bioinformatics tools.

Results: Low total homocysteine was detected in the blood spot, while homocysteine and uric acid levels were normal in the plasma. -sulfocysteine was abnormally elevated in urine. A follow-up examination revealed several progressive neuropathological findings. Also, intermittent convulsions and axial dystonia were observed. However, the coordination of sucking and swallowing was slightly improved. A novel paternal nonsense variant c.475G > T (p.Glu159) and a novel maternal missense variant c.1201A > G (p.Lys401Glu) in were identified in this case by co-segregation verification.

Conclusion: This is the second report of early-onset ISOD case in a non-consanguineous Chinese mainland family. Combined with the clinical characteristics and biochemical indexes, we speculated that these two novel pathogenic variants of the gene underlie the cause of the disease in this patient. Next-generation sequencing (NGS) and Sanger sequencing provided reliable basis for clinical and prenatal diagnoses of this family, it also enriched the mutation spectrum of the gene.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.607085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139553PMC
May 2021

A Novel pH-Sensitive Multifunctional DNA Nanomedicine: An Enhanced and Harmless GD2 Aptamer-Mediated Strategy for Guiding Neuroblastoma Antitumor Therapy.

Int J Nanomedicine 2021 10;16:3217-3240. Epub 2021 May 10.

Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

Background: GD2 is a mainstream biomarker for neuroblastoma (NB)-targeted therapy. Current anti-GD2 therapeutics exhibit several side effects since GD2 is also expressed at low levels on normal cells. Thus, current anti-GD2 therapeutics can be compromised by the coexistence of the target receptor on both cancer cells and normal cells.

Propose: Aptamers are promising and invaluable molecular tools. Because of the pH difference between tumor and normal cells, in this study, we constructed a pH-sensitive aptamer-mediated drug delivery system (IGD-Targeted).

Methods: In vivo Systematic Evolution of Ligands by Exponential Enrichment (SELEX) was used to generate a novel GD2 aptamer. Flow cytometry and molecular docking were applied to assess the binding specificities, affinities abilities of the aptamers. Confocal microscope, CCK8 assay, and BrdU assay were utilized to evaluate whether IGD-Targeted could only bind with GD2 at acidic environment. To evaluate whether IGD-Targeted could inhibit GD2-positive tumor and protect normal cells, in vivo living imaging, histomorphological staining, blood test, and RNA-sequencing were observed in animal model.

Results: GD2 aptamer termed as DB67 could bind with GD2-positive cells with high specificity, while has minimal cross-reactivities to other negative cells. It has been validated that the i-motif in IGD-Targeted facilitates the binding specificity and affinity of the GD2 aptamer to GD2-positive NB tumor cells but does not interfere with GD2-positive normal cells at the pH of the cellular microenvironment. In addition, IGD-Targeted is capable of delivering Dox to only GD2-positive NB tumor cells and not to normal cells in vivo and in vitro, resulting in precise inhibition of tumor cells and protection of normal cells.

Conclusion: This study suggests that IGD-Targeted as a promising platform for NB therapy which could show greater tumor inhibition and fewer side effects to normal cells, regardless of the existence of the same receptor on the target and nontarget cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJN.S302450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121684PMC
May 2021

Environmental Aspects of Oxide Nanoparticles: Probing Oxide Nanoparticle Surface Processes Under Different Environmental Conditions.

Annu Rev Anal Chem (Palo Alto Calif) 2021 07 27;14(1):489-514. Epub 2021 Jul 27.

Department of Nanoengineering, University of California, San Diego, La Jolla, California 92093, USA; email:

Surface chemistry affects the physiochemical properties of nanoparticles in a variety of ways. Therefore, there is great interest in understanding how nanoparticle surfaces evolve under different environmental conditions of pH and temperature. Here, we discuss the use of vibrational spectroscopy as a tool that allows for in situ observations of oxide nanoparticle surfaces and their evolution due to different surface processes. We highlight oxide nanoparticle surface chemistry, either engineered anthropogenic or naturally occurring geochemical nanoparticles, in complex media, with a focus on the impact of () pH on adsorption, intermolecular interactions, and conformational changes; () surface coatings and coadsorbates on protein adsorption kinetics and protein conformation; () surface adsorption on the temperature dependence of protein structure phase changes; and () the use of two-dimensional correlation spectroscopy to analyze spectroscopic results for complex systems. An outlook of the field and remaining challenges is also presented.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1146/annurev-anchem-091420-092928DOI Listing
July 2021

Identification of miRNA-Mediated Subpathways as Prostate Cancer Biomarkers Based on Topological Inference in a Machine Learning Process Using Integrated Gene and miRNA Expression Data.

Front Genet 2021 24;12:656526. Epub 2021 Mar 24.

The Higher Educational Key Laboratory for Measuring and Control Technology and Instrumentations of Heilongjiang Province, Harbin University of Science and Technology, Harbin, China.

Accurately identifying classification biomarkers for distinguishing between normal and cancer samples is challenging. Additionally, the reproducibility of single-molecule biomarkers is limited by the existence of heterogeneous patient subgroups and differences in the sequencing techniques used to collect patient data. In this study, we developed a method to identify robust biomarkers (i.e., miRNA-mediated subpathways) associated with prostate cancer based on normal prostate samples and cancer samples from a dataset from The Cancer Genome Atlas (TCGA; = 546) and datasets from the Gene Expression Omnibus (GEO) database ( = 139 and = 90, with the latter being a cell line dataset). We also obtained 10 other cancer datasets to evaluate the performance of the method. We propose a multi-omics data integration strategy for identifying classification biomarkers using a machine learning method that involves reassigning topological weights to the genes using a directed random walk (DRW)-based method. A global directed pathway network (GDPN) was constructed based on the significantly differentially expressed target genes of the significantly differentially expressed miRNAs, which allowed us to identify the robust biomarkers in the form of miRNA-mediated subpathways (miRNAs). The activity value of each miRNA-mediated subpathway was calculated by integrating multiple types of data, which included the expression of the miRNA and the miRNAs' target genes and GDPN topological information. Finally, we identified the high-frequency miRNA-mediated subpathways involved in prostate cancer using a support vector machine (SVM) model. The results demonstrated that we obtained robust biomarkers of prostate cancer, which could classify prostate cancer and normal samples. Our method outperformed seven other methods, and many of the identified biomarkers were associated with known clinical treatments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.656526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024646PMC
March 2021

Tunable ultrasharp terahertz plasma edge in a lightly doped narrow-gap semiconductor.

Opt Express 2021 Mar;29(6):9261-9268

Plasma edges in metals typically occur in the visible range, producing characteristic colors of metals. In a lightly doped semiconductor, the plasma edge can occur in the terahertz (THz) frequency range. Due to low scattering rates and variable electron densities in semiconductors, such THz plasma edges can be extremely sharp and greatly tunable. Here, we show that an ultrasharp THz plasma edge exists in a lightly n-doped InSb crystal with a record-high transmittance slope of 80 dB/THz. The frequency at which this sharp edge happens can be readily tuned by changing the temperature, electron density, scattering rate, and sample thickness. The edge frequency exhibited a surprising increase with decreasing temperature below 15 K, which we explain as a result of a weak-to-strong transition in the scattering rate, going from ωτ  ≫ 1 to ωτ ∼ 1. These results indicate that doped narrow-gap semiconductors provide a versatile platform for manipulating THz waves in a controllable manner, especially as a high-pass filter with an unprecedented on/off ratio.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.418624DOI Listing
March 2021

Vegetation feedback causes delayed ecosystem response to East Asian Summer Monsoon Rainfall during the Holocene.

Nat Commun 2021 03 23;12(1):1843. Epub 2021 Mar 23.

Key Laboratory of Meteorological Disaster, Ministry of Education (KLME)/Joint International Research Laboratory of Climate and Environment Change (ILCEC)/Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters (CIC-FEMD), Nanjing University of Information Science and Technology, Nanjing, China.

One long-standing issue in the paleoclimate records is whether East Asian Summer Monsoon peaked in the early Holocene or mid-Holocene. Here, combining a set of transient earth system model simulations with proxy records, we propose that, over northern China, monsoon rainfall peaked in the early Holocene, while soil moisture and tree cover peaked in the mid-Holocene. The delayed ecosystem (soil moisture and tree cover) response to rainfall is caused by the vegetation response to winter warming and the subsequent feedback with soil moisture. Our study provides a mechanism for reconciling different evolution behaviors of monsoon proxy records; it sheds light on the driving mechanism of the monsoon evolution and monsoon-ecosystem feedback over northern China, with implications to climate changes in other high climate sensitivity regions over the globe.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22087-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988120PMC
March 2021

[Isovaleric acidemia due to compound heterozygous variants of IVD gene in a case].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Feb;38(2):150-153

Shaanxi Provincial Institute of Pediatrics, Xi'an Children's Hospital, Xi'an, Shaanxi 710002, China. com.

Objective: To analyze the clinical features, biochemical characteristics and molecular pathogenesis of a girl with isovaleric acidemia.

Methods: Clinical features, blood spot amino acid profiles and urinary organic acid profiles of the patient were analyzed. Targeted capture, next generation sequencing and Sanger sequencing were carried out to detect potential variant of the IVD gene.

Results: The patient presented with poor weight gain, poor feeding, lethargy, and a "sweaty feet" odor 10 days after birth. Biochemical test suggested hyperammonemia. Blood spot amino acid profiles displayed a dramatic increase in isovalerylcarnitine (C5: 3. 044, reference range 0.04 - 0.4 μmol/L). Organic acid analysis of her urine sample revealed a high level of isovaleric glycine (669. 53, reference range 0 - 0.5). The child was ultimately diagnosed with isovaleric acidemia, and was found to harbor a paternally derived heterozygous variant c.149G>A (p.R50H) and a maternally derived heterozygous variant c.1123G>A (p.G375S) of the IVD gene. Her elder brother was a heterozygous carrier of c.1123G>A (p.G375S) variant. The c.149G>A (p.R50H) was a known pathogenic variant, while the c.1123G>A (p.G375S) variant was previously unreported.

Conclusion: The pathogenesis of the patient was delineated from the perspective of genetics, which has provided a basis for clinical diagnosis, treatment as well as genetic counseling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.cn511374-20200415-00271DOI Listing
February 2021

Anti-apoptotic efficacy of Qingnao Yizhi formula in hypoxia/reoxygenation primary cortical neurons through the promotion of synaptic plasticity by modulating the NogoA-Nogo receptor/Rho-Rho kinase signaling pathway.

J Tradit Chin Med 2021 02;41(1):59-67

Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Objective: To evaluate the anti-apoptotic efficacy of Qingnao Yizhi formula (,QNYZ) in cultured cerebral cortical neuronal cells (CNCs) and the regulation of the NogoA-Nogo receptor (NgR)/Rho-Rho kinase (ROCK) signaling pathway.

Methods: Primary cultured CNCs were randomly divided into the following groups: normal control group (N-C), hypoxia-reoxygenation group (H/R), high-dose QNYZ group (Q-H), low-dose QNYZ group (Q-L) butylphthalide (NBP) group, and Y-27632 (a selective ROCK transduction pathway inhibiter) group. Except those in the N-C group, CNCs were placed in hypoxic conditions for 24 h and then in reoxygenation conditions for 24 h. Cell media was changed every 48 h, and various assays were performed on the 7th day. Cell viability was evaluated by measuring mitochondrial dehydrogenase activity, using a CCK-8 assay, in triplicate. Synapsin (SYN) protein concentrations were evaluated by enzyme-linked immunosorbent assay. NogoA and RhoA protein expression were evaluated through Western blotting. The gene expression of NogoA, NgR, RhoA, and ROCK was evaluated by reverse transcription-polymerase chain reaction. Cell apoptosis was measured using a terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling assay.

Results: Compared with the N-C group, the cell viability of the H/R group decreased significantly (P < 0.05). The cell viability values for the Q-H and Q-L groups increased compared with that for the H/R group, and the difference was significant for the Q-H group (P < 0.05). The NogoA and RhoA protein levels and the NogoA, NgR, RhoA, and ROCK mRNA expression levels increased in the H/R group, compared with the N-C group, and decreased significantly in the Q-H and Q-L groups (P < 0.05) and in the Y-27632 group (P < 0.05) compared with the H/R group. The SYN levels in the Q-H, Q-L, and NBP groups significantly increased compared with that in the H/R group (P < 0.05). Compared with the H/R group, the numbers of apoptotic cells in the Q-H, Q-L, and NBP groups significantly decreased (P < 0.05).

Conclusion: The presented study demonstrated that QNYZ exerted anti-apoptotic effects on H/R-induced CNCs, possibly through the modulation of the NogoA-NgR/Rho-ROCK signaling pathway and the promotion of synaptic plasticity in H/R CNCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.19852/j.cnki.jtcm.2021.01.008DOI Listing
February 2021

Ferulic acid alleviates lipopolysaccharide-induced acute lung injury through inhibiting TLR4/NF-κB signaling pathway.

J Biochem Mol Toxicol 2021 Mar 2;35(3):e22664. Epub 2020 Nov 2.

Department of Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

Ferulic acid (FA) exhibits anti-inflammatory, antidiabetic, antihyperlipidemic, antioxidant, neuroprotective, and antihypertensive effects. This study aimed to determine whether FA could ameliorate lipopolysaccharide (LPS)-induced inflammatory responses and acute lung injury (ALI) in mice. Mice were challenged with LPS intratracheally to induce ALI 1 h after 3 days of FA (25, 50, and 100 mg/kg) or dexamethasone (DEX; 5 mg/kg) administration. The lung tissues and bronchoalveolar lavage fluid (BALF) were collected 12 h after the LPS challenge. Pretreatment with FA or DEX could attenuate lung histopathological change, complement deposition, and lung wet-to-dry weight ratio of mice injured by LPS. Meanwhile, the influx of neutrophils and macrophages, as well as the production of proinflammatory cytokine (tumor necrosis factor-alpha, interleukin 1 beta [IL-1β], and IL-6), in BALF of ALI mice was significantly decreased. Moreover, FA or DEX markedly reversed the LPS-induced elevation of myeloperoxidase activity and monocyte chemoattractant protein-1 level in lung tissues of ALI mice. In addition, the Western blot analysis demonstrated that FA or DEX effectively inhibited the LPS-induced activation of the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling pathway in lung tissues. The current study suggested that the alleviating effect of FA against LPS-induced ALI might be partially due to the inhibition of the inflammatory response via inactivation of the TLR4/NF-κB signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jbt.22664DOI Listing
March 2021

CuS nanoparticles in humid environments: adsorbed water enhances the transformation of CuS to CuSO.

Nanoscale 2020 Oct;12(37):19350-19358

Department of Chemistry & Biochemistry, University of California San Diego, La Jolla, CA 92093, USA. and Departments of Nanoengineering, University of California San Diego, La Jolla, CA 92093, USA and Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093, USA.

Covellite copper sulfide nanoparticles (CuS NPs) have attracted immense research interest due to their widespread use in a range of biological and energy applications. As such, it is crucial to understand the transformations of these nanomaterials and how these transformations influence the behavior of these nanoparticles in environmental and biological systems. This study specifically focuses on understanding the role of water vapor and adsorbed water in the transformation of CuS NP surfaces to CuSO4 in humid environments. Surface sulfide ions are oxidized to sulfate by oxygen in the presence of water vapor, as detected by atomic force microscopy based photothermal infrared spectroscopy (AFM-PTIR) and in situ attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. These results show that the transformation of CuS to CuSO4 is highly dependent on relative humidity (RH). While sulfide to sulfate conversion is not observed to a great extent at low RH (<20%), there is significant conversion at higher RH (>80%). X-ray photoelectron spectroscopy (XPS) analysis confirms that sulfide is irreversibly oxidized to sulfate. Furthermore, it shows that initially, the Cu ions possess the original oxidation state similar to the original covellite, i.e. Cu+, but they are oxidized to Cu2+ at higher RH. The formation of CuSO4 has also been confirmed by HRTEM. These analyses show that adsorbed water on the NP surfaces enhances the conversion of sulfide to sulfate and the oxidation of Cu+ to Cu2+ in the presence of molecular oxygen.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr05934jDOI Listing
October 2020

Phonon heat transport in cavity-mediated optomechanical nanoresonators.

Nat Commun 2020 Sep 16;11(1):4656. Epub 2020 Sep 16.

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China.

The understanding of heat transport in nonequilibrium thermodynamics is an important research frontier, which is crucial for implementing novel thermodynamic devices, such as heat engines and refrigerators. The convection, conduction, and radiation are the well-known basic ways to transfer thermal energy. Here, we demonstrate a different mechanism of phonon heat transport between two spatially separated nanomechanical resonators coupled by the cavity-enhanced long-range interactions. The single trajectory for thermalization and non-equilibrium dynamics is monitored in real-time. In the strong coupling regime, the instant heat flux spontaneously oscillates back and forth in the nonequilibrium steady states. The universal bound on the precision of nonequilibrium steady-state heat flux, i.e. the thermodynamic uncertainty relation, is verified in such a temperature gradient driven far-off equilibrium system. Our results give more insight into the heat transfer with nanomechanical oscillators, and provide a playground for testing fundamental theories in non-equilibrium thermodynamics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-18426-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494915PMC
September 2020

A high-quality sponge gourd () genome.

Hortic Res 2020 1;7:128. Epub 2020 Aug 1.

Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640 China.

Sponge gourd () is an important cultivated vegetable and medicinal plant in the family Cucurbitaceae. In this study, a draft genome sequence of the sponge gourd inbred line P93075 was analyzed. Using Illumina, PacBio, and 10× Genomics sequencing techniques as well as new assembly techniques such as FALCON and chromatin interaction mapping (Hi-C), a chromosome-scale genome of approximately 656.19 Mb, with an N50 scaffold length of 48.76 Mb, was generated. From this assembly, 25,508 protein-coding gene loci were identified, and 63.81% of the whole-genome consisted of transposable elements, which are major contributors to the expansion of the sponge gourd genome. According to a phylogenetic analysis of conserved genes, the sponge gourd lineage diverged from the bitter gourd lineage approximately 41.6 million years ago. Additionally, many genes that respond to biotic and abiotic stresses were found to be lineage specific or expanded in the sponge gourd genome, as demonstrated by the presence of 462 genes, a much greater number than are found in the genomes of other cucurbit species; these results are consistent with the high stress resistance of sponge gourd. Collectively, our study provides insights into genome evolution and serves as a valuable reference for the genetic improvement of sponge gourd.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41438-020-00350-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395165PMC
August 2020

SIKIAT1/miR-96/FOXA1 axis regulates sepsis-induced kidney injury through induction of apoptosis.

Inflamm Res 2020 Jul 27;69(7):645-656. Epub 2020 Apr 27.

Department of Critical Care Medicine, Shandong Province Linyi Central Hospital, Shandong, China.

Objective And Design: Nowadays, sepsis-induced acute kidney injury (AKI) has gradually become a global problem for its high incidence and increasing mortality. Previous study has reported lncRNA ENST00000452391.1 in sepsis patients. However, its potential biological function and downstream molecular mechanism are still mysterious. METHODS AND RESULTS: Our study found that it was upregulated in sepsis-induced AKI patients, so it was identified as "sepsis-induced kidney injury associated transcript 1 (SIKIAT1)". We used lipopolysaccharide (LPS) stimulated HK-2 cells as an in vitro model to demonstrated that SIKIAT1 acts as a ceRNA for miR-96-3p to enhance FOXA1 expression and promote HK-2 cell apoptosis.

Conclusion: Therefore, it could be a potential biomarker and therapeutic target for sepsis-induced AKI in the development of disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00011-020-01350-0DOI Listing
July 2020

Study of cardiovascular disease prediction model based on random forest in eastern China.

Sci Rep 2020 03 23;10(1):5245. Epub 2020 Mar 23.

Zhejiang Provincial Center for Cardiovascular Disease Control and Prevention, Zhejiang Hospital, Hangzhou, 310013, China.

Cardiovascular disease (CVD) is the leading cause of death worldwide and a major public health concern. CVD prediction is one of the most effective measures for CVD control. In this study, 29930 subjects with high-risk of CVD were selected from 101056 people in 2014, regular follow-up was conducted using electronic health record system. Logistic regression analysis showed that nearly 30 indicators were related to CVD, including male, old age, family income, smoking, drinking, obesity, excessive waist circumference, abnormal cholesterol, abnormal low-density lipoprotein, abnormal fasting blood glucose and else. Several methods were used to build prediction model including multivariate regression model, classification and regression tree (CART), Naïve Bayes, Bagged trees, Ada Boost and Random Forest. We used the multivariate regression model as a benchmark for performance evaluation (Area under the curve, AUC = 0.7143). The results showed that the Random Forest was superior to other methods with an AUC of 0.787 and achieved a significant improvement over the benchmark. We provided a CVD prediction model for 3-year risk assessment of CVD. It was based on a large population with high risk of CVD in eastern China using Random Forest algorithm, which would provide reference for the work of CVD prediction and treatment in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-62133-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7090086PMC
March 2020

Absolute Frequency Measurement of ^{6}Li D Lines with khz-Level Uncertainty.

Phys Rev Lett 2020 Feb;124(6):063002

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, People's Republic of China.

We report the precision measurement of the absolute frequencies, hyperfine splitting, and 2P fine structure splitting in cold atoms of ^{6}Li. Using the stabilized optical frequency comb and developed heterodyne detection technique, the photon shot-noise limited optical spectroscopy is achieved. The measurement of absolute frequencies of D_{1} lines is reached with an uncertainty of about 1 kHz, which is 1 order of magnitude more accurate than previous measurements. The hyperfine splitting of the D_{1} line and 2P fine structure splitting of ^{6}Li are 26.103 1 (14) and 10 052.780 4 (18) MHz, respectively, in agreement with recent theoretical calculations. Our results could provide a benchmark to test the theory at the higher precision and help to resolve large discrepancies among previous experiments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevLett.124.063002DOI Listing
February 2020

Lubricated steady sliding of a rigid sphere on a soft elastic substrate: hydrodynamic friction in the Hertz limit.

Soft Matter 2020 Mar 26;16(11):2760-2773. Epub 2020 Feb 26.

Department of Mechanical and Aerospace Engineering, Field of Theoretical and Applied Mechanics, Cornell University, Ithaca, NY 14853, USA.

Lubricated sliding on soft elastic substrates occurs in a variety of natural and technological settings. It very often occurs in the iso-viscous elasto-hydrodynamic lubrication (EHL) regime (e.g., soft solid, low pressure). In this regime, for sliding of a smooth sphere on a soft solid, a "Hertz-like" effective contact region forms. Much of the fluid is squeezed out of the contact region although enough is retained to keep the solid surfaces fully separated. This is accompanied by complex deformation of the soft solid. The behavior of such soft lubricated contacts is controlled by a single dimensionless parameter 1/β that can be interpreted as a normalized sliding velocity. Solving this fundamental soft-lubrication problem poses significant computational difficulty for large β, which is the limit relevant for soft solids. As a consequence, little is known about the structure of the flow field under soft lubrication in the intake and outlet regions. Here we present a new solution of this soft lubrication problem focusing on the "Hertz" limit. We develop a formulation in polar coordinates that handles difficult computational issues much better than previous methods. We study how hydrodynamic pressure, film thickness and hydrodynamic friction vary with β. Scaling laws for these relationships are given in closed form for a range of β not previously accessible theoretically but that is typical in applications. The computational method presented here can be used to study other soft lubrication problems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9sm02447fDOI Listing
March 2020

A Novel LiDAR Data Classification Algorithm Combined CapsNet with ResNet.

Sensors (Basel) 2020 Feb 19;20(4). Epub 2020 Feb 19.

Department of Computer Science, Chubu University, Aichi 487-8501, Japan.

LiDAR data contain feature information such as the height and shape of the ground target and play an important role for land classification. The effect of convolutional neural network (CNN) for feature extraction on LiDAR data is very significant, however CNN cannot resolve the spatial relationship of features adequately. The capsule network (CapsNet) can identify the spatial variations of features and is widely used in supervised learning. In this article, the CapsNet is combined with the residual network (ResNet) to design a deep network-ResCapNet for improving the accuracy of LiDAR classification. The capsule network represents the features by vectors, which can account for the direction of the features and the relative position between the features. Therefore, more detailed feature information can be extracted. ResNet protects the integrity of information by passing input information to the output directly, which can solve the problem of network degradation caused by information loss in the traditional CNN propagation process to a certain extent. Two different LiDAR data sets and several classic machine learning algorithms are used for comparative experiments. The experimental results show that ResCapNet proposed in this article `improve the performance of LiDAR classification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s20041151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071473PMC
February 2020

Self-Organized Synchronization of Phonon Lasers.

Phys Rev Lett 2020 Feb;124(5):053604

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, China.

Self-organized synchronization is a ubiquitous collective phenomenon, in which each unit adjusts their rhythms to achieve synchrony through mutual interactions. The optomechanical systems, due to their inherently engineerable nonlinearities, provide an ideal platform to study self-organized synchronization. Here, we demonstrate the self-organized synchronization of phonon lasers in a two-membrane-in-the-middle optomechanical system. The probe of each individual membrane enables us to monitor the real-time transient dynamics of synchronization, which reveals that the system enters into the synchronization regime via a torus birth bifurcation line. The phase-locking phenomenon and the transition between in-phase and antiphase regimes are directly observed. Moreover, such a system greatly facilitates the controllable synchronous states, and consequently a phononic memory is realized by tuning the system parameters. This result is an important step towards the future studies of many-body collective behaviors in multiresonator optomechanics with long distances, and might find potential applications in quantum information processing and complex networks.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1103/PhysRevLett.124.053604DOI Listing
February 2020

3D Measurement of Human Chest and Abdomen Surface Based on 3D Fourier Transform and Time Phase Unwrapping.

Sensors (Basel) 2020 Feb 17;20(4). Epub 2020 Feb 17.

The Higher Educational Key Laboratory for Measuring & Control Technology and Instrumentations of Heilongjiang Province, Harbin University of Science and Technology, Harbin 150080, China.

Monitoring respiratory movements is an effective way to improve radiotherapy treatments of thoracic and abdominal tumors, but the current approach is limited to measuring specific points in the chest and abdomen. In this paper, a dynamic three-dimensional (3D) measurement approach of the human chest and abdomen surface is proposed, which can infer tumor movement more accurately, so the radiotherapy damage to the human body can be reduced. Firstly, color stripe patterns in the RGB color model are projected, then after color correction, the collected stripe image sequences are separated into the three RGB primary color stripe image sequences. Secondly, a fringe projection approach is used to extract the folded phase combined 3D Fourier transform with 3D Gaussian filtering. By the relationship between adjacent fringe images in the time sequence, Gaussian filter parameters with individual characteristics are designed and optimized to improve the accuracy of wrapped phase extraction. In addition, based on the difference between the fractional parts of the folded phase error, one remainder equation can be determined, which is used for time-phase unwrapping. The simulation model and human experiments show that the proposed approach can obtain the 3D image sequences of the chest and abdomen surface in respiratory motion effectively and accurately with strong anti-interference ability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s20041091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071058PMC
February 2020

Inconsistency Calibrating Algorithms for Large Scale Piezoresistive Electronic Skin.

Micromachines (Basel) 2020 Feb 3;11(2). Epub 2020 Feb 3.

School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou 350116, China.

In the field of safety and communication of human-robot interaction (HRI), using large-scale electronic skin will be the tendency in the future. The force-sensitive piezoresistive material is the key for piezoresistive electronic skin. In this paper, a non-array large scale piezoresistive tactile sensor and its corresponding calibration methods were presented. Because of the creep inconsistency of large scale piezoresistive material, a creep tracking compensation method based on K-means clustering and fuzzy pattern recognition was proposed to improve the detection accuracy. With the compensated data, the inconsistency and nonlinearity of the sensor was calibrated. The calibration process was divided into two parts. The hierarchical clustering algorithm was utilized firstly to classify and fuse piezoresistive property of different regions over the whole sensor. Then, combining the position information, the force detection model was constructed by Back-Propagation (BP) neural network. At last, a novel flexible tactile sensor for detecting contact position and force was designed as an example and tested after being calibrated. The experimental results showed that the calibration methods proposed were effective in detecting force, and the detection accuracy was improved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/mi11020162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074581PMC
February 2020

Enhancement of elastohydrodynamic friction by elastic hysteresis in a periodic structure.

Soft Matter 2020 Feb;16(6):1627-1635

Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA, USA.

Lubricated contacts are present in many engineering and biological systems involving soft solids. Typical mechanisms considered for controlling the sliding friction in such lubricated conditions involve bulk material compliance, fluid viscosity, viscoelastic response of the material (hysteretic friction), and breaking of the fluid film where dry contact occurs (adhesive friction). In this work we show that a two-phase periodic structure (TPPS), with a varying modulus across the sliding surface, provides significant enhancement of lubricated sliding friction when the system is in the elastohydrodynamic lubrication (EHL) regime. We propose that the enhanced friction is due to extra energy loss during periodic transitions of the sliding indenter between the compliant and stiff regions during which excess energy is dissipated through the fluid layer. This is a form of elastic hysteresis that provides a novel mechanism for friction enhancement in soft solids under lubricated conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9sm02087jDOI Listing
February 2020

A systematic review of the effectiveness and safety of Chinese herbal medicine formula Gualou Xiebai Banxia (GLXBBX) decoction for the treatment of stable angina pectoris.

Medicine (Baltimore) 2019 Dec;98(51):e18375

Department of Cardiovascular Disease, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical of Guangzhou University of Chinese Medicine.

Background: A growing number of studies suggest that Gualou Xiebai Banxia (GLXBBX) decoction, a well-known Chinese herbal formula, has beneficial effects on eliminating angina pectoris symptoms and improving condition of stable angina pectoris (SAP) patients. However, whether this treatment is effective and safe for SAP or not, evidence supporting the effectiveness and safety of this treatment is still incomplete. Besides, there is lack of systematic review to assess the detailed situation (including risk of bias and methodology) of current related clinical studies. This study aimed to evaluate the effectiveness and safety of GLXBBX in treating SAP.

Methods: The major databases (MEDLINE, Embase, the Cochrane Library, China National Knowledge Infrastructure (CNKI), Chinese Scientific Journals Database (VIP) Database, Chinese Biomedical Database (CBM), Chinese Biomedical Literature Service System (SinoMed), and Wanfang Database) were searched from inception to March 2019. Randomized controlled trials (RCTs) of GLXBBX alone or combined with conventional drugs against conventional drugs for SAP were identified. Two assessors reviewed each trial independently. The methodological quality of the eligible studies was evaluated according to the Cochrane Collaboration's tool for assessing risk of bias. Both the data extraction and the literature quality screening evaluation were conducted independently by 2 researchers.

Result: Totally 17 clinical RCTs were included in this study, involving 1676 patients. Due to the high probability of bias of the included studies, it was inappropriate to undertake a meta-analysis. Thus, we only conducted a systematic review and mainly discussed the methodology and limitation of the included studies.

Conclusion: Although the current evidence prompted that GLXBBX might benefit SAP patients in improvement of angina pectoris, ECG, and blood lipid on a certain extent, this systematic review revealed no definite conclusion about the application of GLXBBX for SAP due to the poor methodological quality, high risk of bias, and inadequate reporting on clinical data. More rigorous, multicenter, sufficient-sample, and double-blind randomized clinical trials are warranted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000018375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940126PMC
December 2019

Effects of XIAP on high fat diet-induced hepatic steatosis: a mechanism involving NLRP3 inflammasome and oxidative stress.

Aging (Albany NY) 2019 12 16;11(24):12177-12201. Epub 2019 Dec 16.

Research Center of Brain Intellectual Promotion and Development for Children Aged 0-6 Years, Chongqing University of Education, Chongqing 400067, China.

Increasing evidence indicates that prolonged fat-rich diet (HFD) ingestion is a predisposing factor for metabolic disorder-associated system inflammation and oxidative stress injury, which contributes to the occurrence of non-alcoholic fatty liver disease (NAFLD). NACHT, LRR and PYD domains-containing protein 3 (NLRP3)-mediated inflammatory infiltration was determined to participate in NAFLD. X-linked inhibitor of apoptosis protein (XIAP) was recently confirmed as an essential regulator for apoptosis in cells. However, the role of XIAP in HFD-induced NAFLD is still not understood. Here, XIAP was characterized with respect to HFD-induced NLRP3 inflammasome activation and reactive oxygen species (ROS) generation and palmitate (PA)-treated cells . After HFD administration, hepatic injury was confirmed via histological assessment (grading and staging of NAFLD) and biochemical parameters, oxidative stress, and reduced antioxidant activity. Up-regulated hepatic dysfunction were further indicated by elevated dyslipidemia, lipid accumulation, and decreased fatty acid β-oxidation associated gene expression. Moreover, in the absence of XIAP, NLRP3 signaling activated by HFD-triggered oxidative stress was up-regulated, accompanied by reduction in antioxidants including HO-1, NQO-1, GST, SOD and Nrf2 activity. The detrimental effects of XIAP blocking on hepatic steatosis and related pathologies were also confirmed in PA-treated mouse liver cells. In contrast, overexpression of XIAP by transfection restrained PA-stimulated hepatic steatosis by suppression of oxidative stress, NLRP3 related inflammatory response, and impairment of Nrf2 activity, further alleviating abnormal metabolic disorder associated NAFLD. Taken together, the present study helped to elucidate how HFD-induced hepatic steatosis was regulated by XIAP, possibly via the inhibition of NLRP3 signaling and oxidative stress injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.102559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949096PMC
December 2019

Novel Aptamer-Functionalized Nanoparticles Enhances Bone Defect Repair By Improving Stem Cell Recruitment.

Int J Nanomedicine 2019 6;14:8707-8724. Epub 2019 Nov 6.

Shaanxi Institute of Pediatric Diseases, Xi'an Children's Hospital, Xi'an, Shaanxi 86-710003, People's Republic of China.

Background: The restoration and repair method in the clinic of delayed fracture healing and non-union after comminuted fractures are urgently needed to improve the prognosis of patients. The recruitment of endogenous stem cells has been considered a promising approach in bone defect repair.

Propose: The aim of this study was to generate a de novel MSCs aptamer and developed the first, feasible, economical, bio-compatible, and functional MSCs aptamer-directed nanoparticles without complex manufacture to recruit mesenchymal stem cells (MSCs) for bone defect regeneration.

Methods: Whole-cell SELEX was used to generate a de novel MSCs aptamer. Flow cytometry was applied to assess the binding specificities, affinities and sorting abilities of the aptamers. Nano-Aptamer Ball (NAB) was constructed by NHS/EDC reaction. The diameter and zeta of NAB were assessed by dynamic light scattering. CCK8 assay was utilized to evaluate whether NAB could cause non-specific cytotoxicity and induce cell proliferation. To evaluate the bone repair capacity of NAB, histomorphological staining, alizarin red and micro X-ray were used to observe the repair degree of defect in vivo. ELISA was used to detect osteopontin (OPN), osteocalcin (BGP) by, and alkaline phosphatase (ALP) in peripheral blood.

Results: MSCs aptamer termed as HM69 could bind with MSCs with high specificity and Kd of 9.67 nM, while has minimal cross-reactivities to other negative cells. HM69 could capture MSCs with a purity of >89%. In vitro, NAB could bind and capture MSCs effectively, whereas did not cause obvious cytotoxicity. In vivo, serum OPN, BGP, and ALP levels in the NAB group of rats were increased at both 2 and 4 weeks, indicating the repair and osteogenesis generation. The healing of bone defects in the NAB group was significantly better than control groups, the defects became blurred, and local trabecular bone growth could be observed in X-ray. The organized hematoma and cell growth in the bone marrow of the NAB group were more vigorous in bone sections staining.

Conclusion: These suggested that HM69 and HM69-functionalized nanoparticles NAB exhibited the ability to recruit MSCs both in vitro and in vivo and achieved a better outcome of bone defect repair in a rat model. The findings demonstrate a promising strategy of using aptamer-functionalized bio-nanoparticles for the restoration of bone defects via aptamer-introduced homing of MSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJN.S223164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6847998PMC
March 2020

Corrigendum to "SS30, a novel thioaptamer targeting CD123, inhibits the growth of acute myeloid leukemia cells" [Life Sci. 232 (2019) 116663].

Life Sci 2020 Jan 30;240:117111. Epub 2019 Nov 30.

Shaanxi Institute of Pediatric Diseases, Xi'an Children's Hospital, Xi'an, Shaanxi 0086-710003, PR China; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 0086-710061, PR China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2019.117111DOI Listing
January 2020

Histone deacetylase inhibition up-regulates MHC class I to facilitate cytotoxic T lymphocyte-mediated tumor cell killing in glioma cells.

J Cancer 2019 7;10(23):5638-5645. Epub 2019 Sep 7.

Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

: Immune cells recognize tumor antigens presented on major histocompatibility complex class I (MHC-I) molecule. Increase of MHC-I molecular expression makes tumor cells more susceptible to lysis by immune cells. : Tumor lysate vaccine was prepared to damage glioma cells including cell lines and primary cultured cells from surgical samples. The enhanced effect of histone deacetylase inhibitors (HDACi) to tumor lysate vaccine was observed. The expressions of MHC-I pathway molecules were detected by flow cytometry and western blot after HDACi treatment. Cell apoptosis and cell lysis were measured following blocking cytotoxic T lymphocyte (CTL) pathway. Tumor size and mice survival were analyzed in combinative treatment with HDACi and tumor lysate. : HDACi up-regulated the expressions of MHC-I pathway molecules, and enhanced the recognition and killing of immune cells, which was activated by tumor lysate. Activated antigen specific immune responses regulated CTL activity, and HDACi promoted immune response through cytotoxic effect of CTL. Anti-tumor effect of tumor lysate pulse immunogenicity was elevated by HDACi due to up-regulation of antigen presentation. : Our study showed that HDACi enhanced recognition of glioma cell by immune cells and sensitivity of tumor immunotherapy, and improved the anti-tumor effect of tumor lysate vaccine through activating CTL immune response. These pharmacological molecular mechanisms of increasing immune recognition suggest that epigenetic modulation is a promising strategy for sensitizing immunotherapy for glioma treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.34471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843866PMC
September 2019
-->