Publications by authors named "Haibin Si"

16 Publications

  • Page 1 of 1

Responsive Dual-Targeting Exosome as a Drug Carrier for Combination Cancer Immunotherapy.

Research (Wash D C) 2021 31;2021:9862876. Epub 2021 Aug 31.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China.

Recently, combination immunotherapy, which incorporates the activation of the immune system and inhibition of immune escape, has been proved to be a new powerful strategy for more efficient tumor suppression compared to monotherapy. However, the major challenge is how to integrate multiple immune drugs together and efficiently convey these drugs to tumor sites. Although a variety of nanomaterials have been exploited as carriers for targeting tumor issues and the delivery of multiple drugs, their potential toxicity, immune rejection, and stability are still controversial for clinical application. Here, we proposed endogenic exosomes as drug carriers to deliver two antibodies acting as tumor-targeting molecules and block checkpoint inhibitors with specific response to the tumor microenvironment and costimulatory molecules for further improvement of therapeutic effect. The versatile exosomes exhibit excellent biocompatibility and provide a combination immunotherapy platform with synergistic advantages of activation of immune response and inhibition of immune escape.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.34133/2021/9862876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426567PMC
August 2021

Sputum-Based Tumor Fluid Biopsy: Isolation and High-Throughput Single-Cell Analysis of Exfoliated Tumor Cells for Lung Cancer Diagnosis.

Anal Chem 2021 08 22;93(30):10477-10486. Epub 2021 Jul 22.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.

Timely and effective diagnosis is of great significance for improving the survival rate of lung cancer patients. Although histopathology is the main diagnostic tool among the existing methods for lung cancer diagnosis, it is not suitable for high-risk groups, early lung cancer patients, patients with advanced-stage disease, and other situations wherein tumor tissues cannot be obtained. In view of this, we proposed an innovative lung cancer diagnosis method employing for the first time a microfluidic technology for high-efficiency isolation and high-throughput single-cell analysis of exfoliated tumor cells (ETCs) in sputum. This method fully combines the advantages of traditional sputum cytology and microfluidic technology and realizes the diagnosis of lung cancer by using a small amount of repeatable ETCs instead of the tumor tissue. This method is expected to provide a practical strategy for the non-invasive detection of lung cancer patients and lung cancer screening for high-risk groups.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.1c00833DOI Listing
August 2021

Consecutive Sorting and Phenotypic Counting of CTCs by an Optofluidic Flow Cytometer.

Anal Chem 2019 11 14;91(21):14133-14140. Epub 2019 Oct 14.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science , Shandong Normal University , Jinan 250014 , P. R. China.

Circulating tumor cell (CTC) analysis has been approved for cancer diagnosis and monitoring. However, efficient sorting and high-through phenotypic counting of CTCs from peripheral blood is still a challenge. In this manuscript, we propose an optofluidic flow cytometer (OFCM), which integrates a multistage microfluidic chip and a four-color fluorescence detection system. The OFCM can automatically complete CTC separation, 3D focusing in the microchannel, single-cell phenotypic analysis, and counting at 1.2 mL of whole blood/hour. A high recovery greater than 95% was obtained. Using the OFCM, we analyzed the epithelial-to-mesenchymal transition (EMT) phenotype of CTCs in patients with breast cancer and patients with nonsmall cell lung cancer, which proved that the OFCM is adaptable for phenotypic counting of various CTCs based on the fluorescence labeling of varied biomarkers. We believe that this OFCM will provide a convenient and efficient device for clinical liquid biopsy of tumors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.9b04035DOI Listing
November 2019

High-throughput and ultra-sensitive single-cell profiling of multiple microRNAs and identification of human cancer.

Chem Commun (Camb) 2019 Aug;55(70):10404-10407

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong Normal University, Jinan 250014, Shandong, P. R. China.

We established an efficient method for single-cell miRNA analysis by droplet microfluidics, which has high sensitivity of single molecule detection and high throughput. Single-cell analysis of multiple miRNAs in various cells shows that miRNA expression is closely related to cancer type. CTC analysis shows that the method is applicable for rare cell analysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9cc05553cDOI Listing
August 2019

Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device.

Anal Chem 2019 09 14;91(17):11078-11084. Epub 2019 Aug 14.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Institute of Molecular and Nano Science , Shandong Normal University , Jinan 250014 , People's Republic of China.

Single-cell phenotypic profiling of circulating tumor cells (CTCs) in the blood of cancer patients can reveal vital tumor biology information. Even though various approaches have been provided to enrich and detect CTCs, it remains challenging for consecutive CTC sorting, enumeration, and single-cell characterizations. Here, we report an integrated microfluidic device (IMD) for single-cell phenotypic profiling of CTCs that enables automated CTCs sorting from whole blood following continuous single-cell phenotypic analysis while satisfying the requirements of both high purity (92 ± 3%) of cell sorting and high-throughput processing capacity (5 mL whole blood/3 h). Using this new technique we test the phenotypes of individual CTCs collected from xenograft tumor-bearing mice and colorectal (CRC) patients at different tumor stages. We obtained a correlation between CTC characterization and clinical tumor stage and treatment response. The developed IMD offers a high-throughput, convenient, and rapid strategy to study individual CTCs toward minimally invasive cancer therapy prediction and disease monitoring and has the potential to be translated to clinic for liquid biopsy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.9b01647DOI Listing
September 2019

In situ fluorescent profiling of living cell membrane proteins at a single-molecule level.

Chem Commun (Camb) 2019 Apr;55(28):4043-4046

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.

We propose a signal amplification method that enables visualization analysis of membrane proteins on living cells at a single-molecule level. Using the proposed method, we achieved imaging of PTK7 membrane proteins on HeLa cells and analyzed the down-regulated expression of EpCAM on the MCF-7 cell surface during epithelial-mesenchymal transition (EMT).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9cc00244hDOI Listing
April 2019

Dynamic fluorescent imaging analysis of mitochondrial redox in single cells with a microfluidic device.

Biosens Bioelectron 2019 Mar 11;129:132-138. Epub 2019 Jan 11.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, PR China. Electronic address:

The redox balance in cellular mitochondria is closely related to the physiological and pathological processes of the body. When exposed to external stimuli, the redox state in cells changes dynamically, and presents cell heterogeneity, which creates a need for techniques that can make dynamic and reversible visual analysis of redox in mitochondria at single-cell level. Here we describe a method for single-cell redox analysis based on a microfluidic device combing with a reversible fluorescent probe (Cy-O-ebselen), that enables online culture, labelling and dynamic fluorescent imaging analysis of mitochondrial redox (HO/GSH) change. Using this method, we further explored the dynamic changes of mitochondrial redox state after thermal stimulation or combined thermal-drug stimulation, and analysed the heterogeneous response of cells to external stimuli at the single cell level.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2019.01.005DOI Listing
March 2019

Visible Light-Driven Self-Powered Device Based on a Straddling Nano-Heterojunction and Bio-Application for the Quantitation of Exosomal RNA.

ACS Nano 2019 02 25;13(2):1817-1827. Epub 2019 Jan 25.

Center for Research at the Bio/nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, UF Genetics Institute and McKnight Brain Institute, Shands Cancer Center , University of Florida , Gainesville , Florida 32611-7200 , United States.

This paper reports the design and fabrication of a self-powered biosensing device based on TiO nanosilks (NSs)@MoS quantum dots (QDs) and demonstrates a bioapplication for the quantitative detection of exosomal RNA ( Homo sapiens HOXA distal transcript antisense RNA, HOTTIP). This self-powered device features enhanced power output compared to TiO NSs alone. This is attributed to the formation of a heterojunction structure with suitable band offset derived from the hybridization between TiO NSs and MoS QDs, i.e., the straddling (Type I) band alignment. The sensitization effect and excellent visible light absorption provided by MoS QDs can prolong interfacial carrier lifetime and improve energy conversion efficiency. This self-powered biosensing device has been successfully applied in quantitative HOTTIP detection through effective hybridization between a capture probe and HOTTIP. The successful capture of HOTTIP leads to a sequential decrease in power output, which is utilized for ultrasensitive quantitative HOTTIP detection, with a linear relationship of power output change versus the logarithm of HOTTIP concentration ranging from 5 fg/mL to 50 000 ng/mL and a detection limit as low as 5 fg/mL. This TiO [email protected] QDs-based nanomaterial has excellent potential for a superior self-powered device characterized by economical and portable self-powered biosensing. Moreover, this self-powered, visible-light-driven device shows promising applications for cancer biomarker quantitative detection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.8b07944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613566PMC
February 2019

In situ fluorescence monitoring of diagnosis and treatment: a versatile nanoprobe combining tumor targeting based on MUC1 and controllable DOX release by telomerase.

Chem Commun (Camb) 2018 Jul;54(59):8277-8280

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan, 250014 Shandong, P. R. China.

We have constructed versatile drug-loaded nanoprobes capable of responding to both MUC1 and telomerase and achieving intracellular drug release. Besides, the synthesized drug-loaded nanoprobes can realize the in situ imaging observation of the whole process of nanoprobes targeting the tumor cell membrane, the transmembrane entering the cytoplasm and the release of DOX into the cell nucleus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8cc05052jDOI Listing
July 2018

Highly Sensitive Fluorescence Imaging of Zn and Cu in Living Cells with Signal Amplification Based on Functional DNA Self-Assembly.

Anal Chem 2018 08 18;90(15):8785-8792. Epub 2018 Jul 18.

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Institute of Molecular and Nano Science , Shandong Normal University , Jinan , 250014 Shandong , People's Republic of China.

Intracellular trace Zn and Cu play important roles in the regulation of cell function. Considering the limitations of existing metal ion detection methods regarding sensitivity and applicability to living cells, an amplification strategy based on functional DNA self-assembly under DNAzyme catalysis to improve the sensitivity of intracellular Zn and Cu imaging is reported. In this process, metal ions as cofactor can activate the catalysis of DNAzyme to shear substrate chains, and each broken substrate chain can initiate consecutive hybridizations of hairpin probes (Hx) labeled with fluorophore, which can reflect the information on a single metal ion with multiple fluorophores. The detection limit can reach nearly 80 pM and high-sensitivity fluorescence imaging of intracellular Zn and Cu can be achieved. The results are important for research on cell function regulation associated with trace Zn and Cu. This approach is also a new way to improve the sensitivity of other trace metal ion imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.7b05268DOI Listing
August 2018

Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips.

Lab Chip 2018 04;18(8):1151-1173

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.

Single-cell analysis of bioactive molecules is an essential strategy for a better understanding of cell biology, exploring cell heterogeneity, and improvement of the ability to detect early diseases. In single-cell analysis, highly efficient single-cell manipulation techniques and high-sensitive detection schemes are in urgent need. The rapid development of fluorescent analysis techniques combined with microfluidic chips have offered a widely applicable solution. Thus, in this review, we mainly focus on the application of fluorescence methods in components analysis on microchips at a single-cell level. By targeting different types of biological molecules in cells such as nucleic acids, proteins, and active small molecules, we specially introduce and comment on their corresponding fluorescent probes, fluorescence labelling and sensing strategies, and different fluorescence detection instruments used in single-cell analysis on a microfluidic chip. We hope that through this review, readers will have a better understanding of single-cell fluorescence analysis, especially for single-cell component fluorescence analysis based on microfluidic chips.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c7lc01333gDOI Listing
April 2018

Simultaneous Single-Cell Analysis of Na, K, Ca, and Mg in Neuron-Like PC-12 Cells in a Microfluidic System.

Anal Chem 2017 04 4;89(8):4559-4565. Epub 2017 Apr 4.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Molecular and Nano Science, Shandong Normal University , Jinan, Shandong 250014, P. R. China.

Various intracellular metal ions have closely related functional roles in the nervous system. An excess or deficiency of essential metal ions can contribute to neurodegenerative diseases. Thus, the detection of various metal ions in neurons is important for diagnosing and monitoring these diseases. In particular, single-cell analysis of multiple metal ions allows us to not only understand the cellular heterogeneity and differentiation but also determine the actual relationships among multiple metal ions in each individual cell. Aiming at the low efficient single-cell manipulation and interference of complex biological matrices within cells in the existing method for single-cell metal ion detection, in this manuscript, we present a convenient, sensitive, and reliable method to simultaneously identify and quantify multiple metal ions at the single-cell level using a microfluidic system. Using the combination of on-chip electrophoresis separation and multicolor fluorescence detection, we achieved the simultaneous analysis of Na, K, Ca, and Mg in single PC-12 cells and studied changes in these four metal ions in Aβ-treated PC-12 cells, which is a model of Alzheimer's disease (AD). The data showed that metal ions imbalances in neuron-like cells may be associated with AD induced by Aβ. This method paves the way for multiple metal ion detection in single neuron-like cells, and the results provide insights regarding synergistic function of multiple metal ions in regulation of neurological diseases at the single-cell level.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.6b05045DOI Listing
April 2017

Synthesis of Few-Atomic-Layer BN Hollow Nanospheres and Their Applications as Nanocontainers and Catalyst Support Materials.

ACS Appl Mater Interfaces 2016 Jan 14;8(3):1578-82. Epub 2016 Jan 14.

School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.

In this work, few-atomic-layer boron nitride (BN) hollow nanospheres were directly synthesized via a modified CVD method followed by subsequent high-temperature degassing treatment. The encapsulated impurities in the hollow nanospheres were effectively removed during the reaction process. The BN shells of most nanospheres consisted of 2-6 atomic layers. Because of the low thickness, the obtained BN hollow nanospheres presented excellent performance in many aspects. For instance, they were demonstrated as useful nanocontainers for controllable multistep release of iodine, which could diffuse and be encapsulated into the few-layer BN hollow nanospheres when heating. They were also promising support materials that could markedly increase the photocatalytic activity of TiO2 nanocrystals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.5b10978DOI Listing
January 2016

Large-Scale Synthesis of Few-Layer F-BN Nanocages with Zigzag-Edge Triangular Antidot Defects and Investigation of the Advanced Ferromagnetism.

Nano Lett 2015 Dec 30;15(12):8122-8. Epub 2015 Nov 30.

School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.

Investigation of light-element magnetism system is essential in fundamental and practical fields. Here, few-layer (∼3 nm) fluorinated hexagonal boron nitride (F-BN) nanocages with zigzag-edge triangular antidot defects were synthesized via a facile one-step solid-state reaction. They are free of metallic impurities confirmed by X-ray photoelectron spectroscopy, electron energy loss spectroscopy, and inductively coupled plasma atomic emission spectroscopy. Ferromagnetism is obviously observed in the BN nanocages. Saturation magnetization values of them differed by less than 7% between 5 and 300 K, indicating that the Curie temperature (Tc) was much higher than 300 K. By adjusting the concentration of triangular antidot defects and fluorine dopants, the ferromagnetic performance of BN nanocages could be effectively varied, indicating that the observed magnetism originates from triangular antidot defects and fluorination. The corresponding theoretical calculation shows that antidot defects and fluorine doping in BN lattice both favor spontaneous spin polarization and the formation of local magnetic moment, which should be responsible for long-range magnetic ordering in the sp material.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.5b03569DOI Listing
December 2015

Pressure-Induced Oriented Attachment Growth of Large-Size Crystals for Constructing 3D Ordered Superstructures.

ACS Nano 2016 Jan 30;10(1):405-12. Epub 2015 Nov 30.

School of Materials Science and Engineering, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.

Oriented attachment (OA), a nonclassical crystal growth mechanism, provides a powerful bottom-up approach to obtain ordered superstructures, which also demonstrate exciting charge transmission characteristic. However, there is little work observably pronouncing the achievement of 3D OA growth of crystallites with large size (e.g., submicrometer crystals). Here, we report that SnO2 3D ordered superstructures can be synthesized by means of a self-limited assembly assisted by OA in a designed high-pressure solvothermal system. The size of primary building blocks is 200-250 nm, which is significantly larger than that in previous results (normally <10 nm). High pressure plays the key role in the formation of 3D configuration and fusion of adjacent crystals. Furthermore, this high-pressure strategy can be readily expanded to additional materials. We anticipate that the welded structures will constitute an ideal system with relevance to applications in optical responses, lithium ion battery, solar cells, and chemical sensing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.5b05108DOI Listing
January 2016

Boron nitride ultrathin fibrous nanonets: one-step synthesis and applications for ultrafast adsorption for water treatment and selective filtration of nanoparticles.

ACS Appl Mater Interfaces 2013 Dec 3;5(24):12773-8. Epub 2013 Dec 3.

State Key Lab of Crystal Materials, Shandong University , Jinan 250100, P. R. China.

Novel boron nitride (BN) ultrathin fibrous networks are firstly synthesized via an one-step solvothermal process. The average diameter of BN nanofibers is only ~8 nm. This nanonets exhibit excellent performance for water treatment. The maximum adsorption capacity for methyl blue is 327.8 mg g(-1). Especially, they present the property of ultrafast adsorption for dye removal. Only ~1 min is enough to almost achieve the adsorption equilibrium. In addition, the BN fibrous nanonets could be applied for the size-selective separation of nanoparticles via a filtration process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/am403789cDOI Listing
December 2013
-->