Publications by authors named "Hai-hua Wang"

53 Publications

Seasonal Changes in Root-Associated Fungal Microbiota Drive N and P Cycling in Terrestrial Ecosystem.

Front Microbiol 2020 18;11:526898. Epub 2021 Jan 18.

College of Forestry, Northwest A&F University, Yangling, China.

In terrestrial ecosystems, mycorrhizal roots play a key role in the cycling of soil carbon (C) and other nutrients. The impact of environmental factors on the mycorrhizal fungal community has been well studied; however, the seasonal variations in the root-associated fungal microbiota affected by environmental changes are less clear. To improve the understanding of how environmental factors shape the fungal microbiota in mycorrhizal roots, seasonal changes in root-associated fungi were investigated. In the present study, the seasonal dynamics of edaphic properties, soil enzymatic activities, root fungal colonization rates, and root-associated fungal microbiota in forests were studied across four seasons during a whole year to reveal their correlations with environmental changes. The results indicate that the soil functions, such as the enzymatic activities related to nitrogen (N) and phosphorus (P) degradation, were varied with the seasonal changes in microclimate factors, resulting in a significant fluctuation of edaphic properties. In addition, the ectomycorrhizal fungal colonization rate in the host pine tree roots increased during warm seasons (summer and autumn), while the fungal colonization rate of dark septate endophyte was declined. Moreover, the present study indicates that the fungal biomass increased in both the pine roots and rhizospheric soils during warm seasons, while the fungal species richness and diversity decreased. While the Basidiomycota and Ascomycota were the two dominant phyla in both root and soil fungal communities, the higher relative abundance of Basidiomycota taxa presented in warm seasons. In addition, the fungal microbial network complexity declined under the higher temperature and humidity conditions. The present study illustrates that the varieties in connectivity between the microbial networks and in functional taxa of root-associated fungal microbiota significantly influence the soil ecosystem functions, especially the N and P cycling.
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http://dx.doi.org/10.3389/fmicb.2020.526898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849022PMC
January 2021

Epigenetic Regulation of Amyloid-beta Metabolism in Alzheimer's Disease.

Curr Med Sci 2020 Dec 11;40(6):1022-1030. Epub 2021 Jan 11.

Hubei University of Chinese Medicine, Wuhan, 430060, China.

Senile plaques (SPs) are one of the pathological features of Alzheimer's disease (AD) and they are formed by the overproduction and aggregation of amyloid-beta (Aβ) peptides derived from the abnormal cleavage of amyloid precursor protein (APP). Thus, understanding the regulatory mechanisms during Aβ metabolism is of great importance to elucidate AD pathogenesis. Recent studies have shown that epigenetic modulation-including DNA methylation, non-coding RNA alterations, and histone modifications-is of great significance in regulating Aβ metabolism. In this article, we review the aberrant epigenetic regulation of Aβ metabolism.
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http://dx.doi.org/10.1007/s11596-020-2283-0DOI Listing
December 2020

Fullerenol as a photoelectrochemical nanoprobe for discrimination and ultrasensitive detection of amplification-free single-stranded DNA.

Biosens Bioelectron 2020 Nov 13;173:112802. Epub 2020 Nov 13.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address:

Traditional approaches for nucleic acids detection require prior amplification of target genes, while nanomaterials-aided DNA biosensors are very magnificent but still suffer from the nanomaterial acquirement and limited sensitivity (above picomolar level). Herein, fullerenol C(OH), a representative fullerene derivative, was employed as a photoelectrochemical (PEC) nanoprobe to achieve discrimination and ultrasensitive detection of amplification-free single-stranded DNA (ssDNA) down to sub-femtomolar level. The bonded hydroxyl groups with intense density endowed fullerenol to directly recognize and capture ssDNA-AuNPs via the hydrogen bonding interactions (H-bonds), leading to a sharply decreased photocurrent with quenching efficiency up to 85%, which could be attributed to the photo-generated electrons on the conduction band of fullerenol (-4.66 eV) preferentially migrating to the Fermi level of AuNPs (-5.1 eV) rather than the electrode. In the presence of target gene (mutant human p53 gene fragment), the H-bonds between fullerenol and ssDNA were competitively depleted during the base pairing process of complete hybridization between ssDNA and target, making double-stranded DNA-AuNPs (dsDNA-AuNPs) depart so that the photocurrent powerfully recovered. On basis of the photocurrent variation before and after target introduction, this proposed simple, rapid and ultrasensitive PEC biosensor for amplification-free target gene detection illustrated a wide liner ranged from 1 fM to 100 pM and a detection limit of 0.338 fM. This work presented an ingenious strategy for the discrimination and ultrasensitive detection of nucleic acids, and the well-designed PEC biosensor was further conducive to the impetus of clinic diagnostics.
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http://dx.doi.org/10.1016/j.bios.2020.112802DOI Listing
November 2020

Typical gene expression profile of pseudorabies virus reactivation from latency in swine trigeminal ganglion.

J Neurovirol 2020 10 15;26(5):687-695. Epub 2020 Jul 15.

State Key Laboratory of Agricultural Microbiology and Key Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

Pseudorabies virus (PRV) establishes a lifelong latent infection in swine trigeminal ganglion (TG) following acute infection. Increased corticosteroid levels, due to stress, increases the incidence of reactivation from latency. Muscle injection combined with intravenous deliver of the synthetic corticosteroid dexamethasone (DEX) consistently induces reactivation from latency in pigs. In this study, PRV-free piglets were infected with PRV. Viral shedding in nasal and ocular swabs demonstrated that PRV infection entered the latent period. The anti-PRV antibody was detected by enzyme-linked immunosorbent assay and the serum neutralization test, which suggested that the PRV could establish latent infection in the presence of humoral immunity. Immunohistochemistry and viral genome detection of TG neurons suggested that PRV was reactivated from latency. Viral gene expressions of IE180, EP0, VP16, and LLT-intron were readily detected at 3-h post-DEX treatment, but gB, a γ1 gene, was not detectable. The differentially expressed phosphorylated proteins of TG neurons were analyzed by ITRAQ coupled with LC-MS/MS, and p-EIF2S2 differentially expression was confirmed by western blot assay. Taken together, our study provides the evidence that typical gene expression in PRV reactivation from latency in TG is disordered compared with known lytic infection in epithelial cells.
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http://dx.doi.org/10.1007/s13365-020-00866-9DOI Listing
October 2020

Development of a Real-Time TaqMan PCR Method for Absolute Quantification of the Biocontrol Agent .

Plant Dis 2020 Jun 20;104(6):1694-1700. Epub 2020 Apr 20.

Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 34134, South Korea.

has been used as an effective biocontrol agent for the management of the pinewood nematode, . Tools for monitoring the colonization and parasitism patterns of are required for the development of highly effective biocontrol strategies. Because the TaqMan PCR technique is effective for quantification of species in environmental samples, a real-time PCR-based methodology was developed for absolute quantification of via internal standard addition and extrapolation of DNA quantity to hyphal length. Primers and a probe for the 28S ribosomal RNA gene of were designed, and nested TaqMan real-time PCR-based quantification was performed. In addition, internal standard-based yield measurement was correlated to the absolute quantity of target genomic DNA. Moreover, an extrapolation curve obtained by optical microscopy and image analysis of the mycelia was constructed for the measurement of fungal hyphal length. The absolute quantification method developed in the present study provides a sensitive and accurate technique to quantify fungal density in either wood or other substrate samples and can be used as an effective tool for future studies of biocontrol agents.
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http://dx.doi.org/10.1094/PDIS-10-19-2076-REDOI Listing
June 2020

In vivo infection of Bursaphelenchus xylophilus by the fungus Esteya vermicola.

Pest Manag Sci 2020 Aug 19;76(8):2854-2864. Epub 2020 May 19.

Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, South Korea.

Background: As the causal agent of pine wilt disease, Bursaphelenchus xylophilus, is a serious pathogen of forest pine trees. Esteya vermicola is a nematophagous fungus of B. xylophilus and exhibits great potential as a biological control agent. However, the in vivo infection mechanism of E. vermicola on B. xylophilus is unclear. Experiments were conducted to study the colonization of host plant and infection of B. xylophilus by E. vermicola inside pine tree xylem.

Results: A green fluorescent protein (GFP)-tagged E. vermicola transformant was constructed as a biomarker to study the in vivo colonization and infection of B. xylophilus in pine trees. The in vitro infection of B. xylophilus by E. vermicola was observed through GFP expression. The bacilloid conidia produced by trophic hyphae in the body of the nematode are described. Additionally, the monitoring of in vivo colonization by GFP-tagged E. vermicola showed the germination and hyphal extension of this fungus after inoculation. Moreover, B. xylophilus infected by this biocontrol agent were extracted from healthy seedlings and observed in the xylem of trees that were wilting due to pine wilt disease.

Conclusion: Evidence of fungal colonization and infection of B. xylophilus by E. vermicola is provided to improve our understanding of the in vivo infection mechanisms used by this nematophagous fungus against B. xylophilus. The infection of B. xylophilus by E. vermicola was inferred to begin with the implantation of propagules, and this inference will require future investigation. The colonization of Esteya vermicola in host pine tree xylem and the in vivo infection of pinewood nematode by E. vermicola were investigated using the green fluorescence protein transformant. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.5839DOI Listing
August 2020

Comparative understanding of metal hyperaccumulation in plants: a mini-review.

Environ Geochem Health 2021 Apr 14;43(4):1599-1607. Epub 2020 Feb 14.

Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Agronomy, Hunan Agricultural University, Changsha, 410128, Hunan, China.

Hyperaccumulator plants are ideal models for investigating the regulatory mechanisms of plant metal homeostasis and environmental adaptation due to their notable traits of metal accumulation and tolerance. These traits may benefit either the biofortification of essential mineral nutrients or the phytoremediation of nonessential toxic metals. A common mechanism by which elevated expression of key genes involved in metal transport or chelation contributes to hyperaccumulation and hypertolerance was proposed mainly from studies examining two Brassicaceae hyperaccumulators, namely Arabidopsis halleri and Noccaea caerulescens (formerly Thlaspi caerulescens). Meanwhile, recent findings regarding systems outside the Brassicaceae hyperaccumulators indicated that functional enhancement of key genes might represent a strategy evolved by hyperaccumulator plants. This review provides a brief outline of metal hyperaccumulation in plants and highlights commonalities and differences among various hyperaccumulators.
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http://dx.doi.org/10.1007/s10653-020-00533-2DOI Listing
April 2021

[TLR4/NF-kappaB p65 signaling pathway mediates protective effect of triptolide on endothelium in rats with endotoxemia].

Zhongguo Zhong Yao Za Zhi 2019 Nov;44(22):4912-4917

Department of Physiology,Wannan Medical College Wuhu 241002,China.

The aim of this paper was to observe the effect of triptolide( TP) on cardiovascular function and its possible mechanism by intraperitoneal injection of bacterial lipopolysaccharide in rats with endotoxemia. Sixty male Sprague-Dawley rats were randomly divided intonormal group( NC group),endotoxemia model group( LPS group),TP low concentration intervention group( LPS + TP-L group,25 μg·kg~(-1)),TP middle concentration intervention group( LPS+TP-M group,50 μg·kg~(-1)),TP high concentration intervention group( LPS+TP-H group,100 μg·kg~(-1)) and polymyxin B group( LPS+PMX-B group,0. 2 mg·kg~(-1)). 10 mg·kg~(-1) LPS was injected intraperitoneally for 6 h to replicate the endotoxemia rat model. The rats in TP intervention groups were pre-treated 15 min before intraperitoneal injection of LPS. Rats in each group underwent total arterial intubation to measure hemodynamic parameters: heart rate( HR),left ventricular diastolic pressure( LVDP),the maximum rate of the increase/decrease of left ventricular pressure( ±dp/dtmax). The levels of BNP,CK-MB and c Tn-Ⅰ in serum and levels of TNF-α and IL-6 in plasma were detected by ELISA. The contents of p65 protein in myocardium and contents of p65,TLR4,i NOS and e NOS protein in thoracic aorta were detected by Western blot. As compared with NC group,the hemodynamic indexes in LPS group were significantly decreased; the contents of BNP,CK-MB and c Tn-Ⅰ in serum,TNF-α and IL-6 in plasma,p65 in myocardium,i NOS,e NOS,TLR4 and p65 in vascular tissues were significantly increased. As compared with LPS group,the hemodynamic indexes were significantly improved in LPS+TP-M group,LPS+TP-H group and LPS+PMX-B group; the contents of BNP,CK-MB and c Tn-Ⅰ in serum,TNF-α and IL-6 in plasma,p65 in myocardium,i NOS,e NOS,TLR4 and p65 in vascular tissues were significantly decreased in each treatment group. Triptolide has a protective effect on cardiovascular damage in a dose-dependent manner in endotoxemia rats,probably through TLR4/NF-κB p65 signaling pathway to improve endothelial function.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20190701.402DOI Listing
November 2019

[Advances in mechanisms of nutrient exchange between mycorrhizal fungi and host plants].

Ying Yong Sheng Tai Xue Bao 2019 Oct;30(10):3596-3604

College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China.

Mycorrhizae, formed through the colonization of soil mycorrhizal fungi into the roots of host plants, are common symbiosis in the terrestrial ecosystems. The establishment of mycorrhizae is mainly based on the bidirectional nutrient exchanges between the symbiotic partners. Mycorrhizal fungi can absorb mineral nutrients, such as nitrogen and phosphorus, from soil and transport them to the host plants for their growth. As an exchange, host plants supply mycorrhizal fungi with the carbohydrates in the form of lipids or sugars, which are essential for fungal growth. In recent years, the mechanism of nutrient exchange between the mycorrhizal fungi and host plants has been a hot research topic. Important progresses have been achieved in mechanisms of host plants nutrient uptake and transport mediated by the mycorrhizal fungi. In this review, recent advances in nutrient exchange between arbuscular mycorrhizal fungi, ectomycorrhizal fungi and host plants were summarized, especially in the absorption and bidirectional transfer mechanisms of important nutrients, such as carbon, nitrogen and phosphorus. The potential regulatory effects of nutrient exchange in the mycorrhizal development were also reviewed. In addition, key problems and prospects of related researches were analyzed. This paper would be meaningful for the establishment of mycorrhizal model and the optimization of mycorrhizal effects.
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http://dx.doi.org/10.13287/j.1001-9332.201910.034DOI Listing
October 2019

p-n-Sensitized Heterostructure CoO/Fullerene with Highly Efficient Photoelectrochemical Performance for Ultrasensitive DNA Detection.

ACS Appl Mater Interfaces 2019 Jul 19;11(26):23765-23772. Epub 2019 Jun 19.

Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering , Southwest University , Chongqing 400715 , People's Republic of China.

Significantly sensitized architectures meeting the requirements of high photoelectric conversion efficiency and promising photocurrent intensity are extremely desirable, but challenges in sensitizer development and efficiency in photoelectrochemical (PEC) fields remain. In this paper, the p-type metal oxide semiconductor CoO was attached as an effective photosensitizer to n-type fullerene C in view of appropriately matched energy band levels to form the highlighted p-n-sensitized heterostructure CoO/fullerene, with facilitated charge separation and accelerated carrier mobility. Compared with traditional p-n heterostructure, the p-n-sensitized heterostructure CoO/fullerene illustrated a wider range for light absorption with further enhanced light-harvesting capability, thereby leading to more exceptional PEC performance containing remarkably promoted photoelectric conversion efficiency and improved photocurrent intensity. Impressively, the photocurrent intensity obtained by CoO/fullerene was about sixfold higher than that of fullerene alone, and this achievement was quite favored compared to the reported works for fullerene sensitization, which could be responsible for the advancement of detection sensitivity for the subsequently constructed biosensor. Unambiguously, given the p-n-sensitized heterostructure CoO/fullerene of high PEC activity, the well-fabricated three-dimensional DNA walker applied as a target-cascade signal amplification strategy, and the Au layer employed as the specific linker between the photoactive material and the signal amplification product, a smart PEC biosensor was successfully enabled for ultrasensitive investigation of the model DNA (a fragment of the p53 gene), showing a wide linear range of 60 to 1 × 10 aM and a detection limit of 20 aM. This proposed PEC biosensor provided acceptable insights into the clinic analysis, disease therapies, and other relevant subjects.
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http://dx.doi.org/10.1021/acsami.9b05923DOI Listing
July 2019

Establishment of a fluorescent PCR melting curve method for detecting asthma susceptibility using gene SNP typing.

J Asthma 2020 08 29;57(8):850-857. Epub 2019 May 29.

Radiology Department, Jining First People's Hospital, Jining City, Shandong Province, PR China.

To develop a detection method for single nucleotide polymorphisms (SNPs) of bronchial asthma (BA) susceptibility genes (, and ) based on fluorescence PCR melting curves. Peripheral blood samples from 33 patients with BA were collected. DNA was extracted, and positive plasmids were constructed. Probes and primers for fluorescence polymerase chain reaction (PCR) were designed according to and sequences, and the SNPs were separately detected by gene sequencing and fluorescence PCR melting curve. The system was successfully divided into 3 SNPs, including , and , and a comparison of sequencing methods showed that the results were completely consistent. The lowest detection limit was 1 ng/reaction, the sensitivity and specificity were 100%, and this method had high repeatability (CV = 2.8%). The fluorescence PCR melting curve method is suitable for the rapid and accurate classification of SNPs. The method is economical, simple, and efficient, and is suitable for the screening of the susceptible gene SNPs in a large-scale population of patients with BA.
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http://dx.doi.org/10.1080/02770903.2019.1619084DOI Listing
August 2020

Storage of Airy wavepackets based on electromagnetically induced transparency.

Opt Express 2019 Mar;27(5):6370-6376

The research of Airy beams has attained much attention due to their unique characteristics. Coherent control of Airy beams is important for further light beam manipulation and information processing. In this paper, we experimentally investigate the storage and retrieval of 2D Airy wavepackets in a solid-state medium driven by electromagnetically induced transparency (EIT). The transverse profile of the weak probe pulse is modulated by Airy wavepackets. Under EIT condition, the probe Airy wavepackets are stored into the experimental medium by manipulating the intensity of the control field, and later retrieved by the opposite process. The retrieved Airy wavepackets keep a high similarity compared with those before the storage. Furthermore, the self-healing property of the retrieved Airy wavepackets is investigated. This storage of Airy wavepackets develops the control method of Airy beams, which will be useful in further applications.
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http://dx.doi.org/10.1364/OE.27.006370DOI Listing
March 2019

[Characteristics of soil organic carbon mineralization in low altitude and high altitude forests in Wuyi Mountains, southeastern China].

Ying Yong Sheng Tai Xue Bao 2018 Mar;29(3):748-756

College of Geographical Science, Fujian Normal University, Fuzhou 350007, China.

Examining the variations of soil organic carbon mineralization at different altitudes is crucial for better understanding of soil organic carbon (SOC) dynamics. We selected the low altitude and high altitude broad-leaved forest soils in Wuyi Mountains as the research object, and incubated them under particular annual average temperature (17 and 9 ℃, respectively) in laboratory to investigate the difference of SOC mineralization characteristics. The results showed that the cumulative SOC mineralization had no significant difference between forest soils at low and high altitude in a 126-day incubation period under ambient temperature. Soil organic carbon content of high altitude soil was significantly higher than that from low altitude. The dynamics of SOC mineralization could fitted by the first-order kinetics. Both mineralization potential (C) and mineralization rate constant (K) values of two soils had no significant difference, but C/SOC value and mineralization ratio were significantly higher at low altitude, indicating that the carbon sequestration capacity of low altitude soil was relatively lower than that of high altitude under ambient temperature. Soil microbial biomass carbon and microbial quotients were significantly higher than that of low altitude with the increase of incubation time, indicating that the ability of microbial carbon assimilation was greater at high altitude. On the other hand, the activities of β-1,4-glucosidase and cellobiohydrolase in high altitude soil were higher, suggesting that more labile carbon would be decomposed by soil microbes. The carbon sequestration capacity and microbial carbon utilization efficiency in high altitude soil would be reduced and thus result in a decline of soil organic carbon storage under the scenarios of climate warming.
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http://dx.doi.org/10.13287/j.1001-9332.201803.015DOI Listing
March 2018

Optically Tunable Gratings Based on Coherent Population Oscillation.

Sci Rep 2018 May 1;8(1):6834. Epub 2018 May 1.

Center for Quantum Sciences, Northeast Normal University, Jingyue Street 2555, Changchun, 130117, China.

We theoretically study the optically tunable gratings based on a L-type atomic medium using coherent population oscillations from the angle of reflection and transmission of the probe field. Adopting a standing-wave driving field, the refractive index of the medium as well as the absorption are periodically modified. Consequently, the Bragg scattering causes the effective reflection. We show that different intensities of the control field lead to three types of reflection profile which actually correspond to different absorption/amplification features of the medium. We present a detailed analyses about the influence of amplification on the reflection profile as well. The coherent population oscillation is robust to the dephasing effect, and such induced gratings could have promising applications in nonlinear optics and all-optical information processing.
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http://dx.doi.org/10.1038/s41598-018-25010-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931600PMC
May 2018

An Uncommon Carboxyl-Decorated Metal-Organic Framework with Selective Gas Adsorption and Catalytic Conversion of CO.

Chemistry 2018 Jan 13;24(4):865-871. Epub 2017 Dec 13.

School of Chemical Engineering, The University of Queensland, Brisbane, 4072, Australia.

A new three-dimensional (3D) framework, [Ni(btzip)(H btzip)]⋅2 DMF⋅2 H O (1) (H btzip=4,6-bis(triazol-1-yl)isophthalic acid) as an acidic heterogeneous catalyst was constructed by the reaction of nickel wire and a triazolyl-carboxyl linker. Framework 1 possesses intersected 2D channels decorated by free COOH groups and uncoordinated triazolyl N atoms, leading to not only high CO and C H adsorption capacity but also significant selective capture for CO and C H over CH and CO in 273-333 K. Moreover, 1 reveals chemical stability toward water. Grand Canonical Monte Carlo simulations confirmed the multiple CO - and C H -philic sites. As a result of the presence of accessible Brønsted acidic COOH groups in the channels, the activated framework demonstrates highly efficient catalytic activity in the cycloaddition reaction of CO with propylene oxide/4-chloromethyl-1,3-dioxolan-2-one/3-butoxy-1,2-epoxypropane into cyclic carbonates.
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http://dx.doi.org/10.1002/chem.201704027DOI Listing
January 2018

Lysobacter zhanggongensis sp. nov. Isolated from a Pit Mud.

Curr Microbiol 2017 Dec 24;74(12):1389-1393. Epub 2017 Aug 24.

Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.

The Gram-stain-negative, rod-shaped and non-motile bacterial strain, designated ZGLJ7-1, was isolated from a pit mud. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain ZGLJ7-1 was related to the genus Lysobacter and had the highest 16S rRNA gene sequence similarity with the type strain of Lysobacter arseniciresistens ZS79 (97.4%). The predominant cellular fatty acids were iso-C, iso-Cω9c, iso-C and iso-C3-OH. Strain ZGLJ7-1 had Q-8 as the predominant ubiquinone. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids and two unidentified lipids. The genomic DNA G+C content of strain ZGLJ7-1 was 69.5 mol%. Strain ZGLJ7-1 shared DNA relatedness with 35% Lysobacter arseniciresistens CGMCC 1.10752. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that the strain ZGLJ7-1 is a representative of a novel species of the genus Lysobacter, for which we propose the name Lysobacter zhanggongensis sp. nov. (type strain ZGLJ7-1 = KACC 18547 = CGMCC 1.15404).
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http://dx.doi.org/10.1007/s00284-017-1330-yDOI Listing
December 2017

Construction of Highly Porous Pillared Metal-Organic Frameworks: Rational Synthesis, Structure, and Gas Sorption Properties.

Inorg Chem 2017 Aug 17;56(15):9147-9155. Epub 2017 Jul 17.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an 710069, P. R. China.

Different from the conventional method to construct pillared-layer metal-organic frameworks (MOFs) by using mixed bipyridyl and dicarboxylate ligands, herein, we present a new approach to build pillared-layer frameworks based on the pyridyldicarboxylate ligands which were predesigned with a certain shape. As exemplified, the ligands of 3-(2',5'-dicarboxyphenyl)benzoic acid (Hdbba) and 3-(2',5'-dicarboxylphenyl)pyridine acid (Hdcpy) were selected and employed to construct three pillared-layer MOFs, [Zn(dbba)(bipy)(DMF)]·3DMF·4HO (1) (bipy = 4,4'-bipyridine), and a pair of crystal polymorphs of [Zn(dcpy)]·1.5DMF·1.5HO (2 and 3), under solvothermal reactions, respectively. In the structures of 1-3, the [Zn(COO)] clusters are bridged by the terephthalate units of dbba/dcpy to form 2D layers; these layers are further pillared by bipy and the benzoate units of dbba or the pyridine units of dcpy to furnish the 3D frameworks. All of them possess high porosity characterized by N adsorption and exhibit high selective adsorption of CH and CO over CH.
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http://dx.doi.org/10.1021/acs.inorgchem.7b01197DOI Listing
August 2017

Porous MOF with Highly Efficient Selectivity and Chemical Conversion for CO.

ACS Appl Mater Interfaces 2017 May 17;9(21):17969-17976. Epub 2017 May 17.

School of Chemical Engineering, The University of Queensland , Brisbane 4072, Australia.

A new Co(II)-based MOF, {[Co(tzpa)(OH)(HO)]·DMF} (1) (Htzpa = 5-(4-(tetrazol-5-yl)phenyl)isophthalic acid), was constructed by employing a tetrazolyl-carboxyl ligand Htzpa. 1 possesses 1D tubular channels that are decorated by μ-OH groups, uncoordinated carboxylate O atoms, and open metal centers generated by the removal of coordinated water molecules, leading to high CO adsorption capacity and significantly selective capture for CO over CH and CO in the temperature range of 298-333 K. Moreover, 1 shows the chemical stability in acidic and basic aqueous solutions. Grand canonical Monte Carlo simulations identified multiple CO-philic sites in 1. In addition, the activated 1 as the heterogeneous Lewis and Brønsted acid bifunctional catalyst facilitates the chemical fixation of CO coupling with epoxides into cyclic carbonates under ambient conditions.
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http://dx.doi.org/10.1021/acsami.7b03835DOI Listing
May 2017

Controlling transverse shift of the reflected light via high refractive index with zero absorption.

Opt Express 2017 May;25(9):10335-10344

We present a theoretical investigation on controlling the transverse shift while most of the researches are on longitudinal Goos-Hänchen shift. A two-layer system is considered. The refractive index of the first layer is fixed. The second layer is an atomic system coupled by a strong laser field to realize the Λ-style electromagnetically induced transparency, and an additional microwave field drives the transition between the lower two levels to construct high refractive index with zero absorption. We use such phenomenon to modify the refractive index, and consequently the transverse shift in reflection. The properties of the atomic system and the transverse shift of reflected field are briefly studied. Our investigation shows that the shift can be tuned by the strength of the microwave field. And since the atomic system is quite sensitive to the phase of the light fields, through which the transverse shift can be manipulated effectively. More importantly, the absorption is limited due to the presence of the microwave field.
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http://dx.doi.org/10.1364/OE.25.010335DOI Listing
May 2017

[Role of changes in sodium pump activity and endoplasmic reticulum stress in the ischemia/reperfusion induced injury of isolated hearts].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2017 Jan;33(1):40-46

Department of Physiology, Wannan Medical College, Wuhu 241002, China.

Objective: To investigate the roles of change in sodium pump activity and endoplasmic reticulum stress (ERS) in ischemia/reperfusion (I/R) injury of the isolated rat hearts.

Methods: Sixty male SD rats were randomly divided into 6 groups (=10 each):normal control group (NC), I/R group (I/R), ouabain-I/R group (OUA-I/R), anti-digoxin antiserum-I/R group (Anti-Dig-I/R), PP2 (Src kinase inhibitor)-ouabain-I/R group (PP2-OUA-I/R),U73122 (PLC inhibitor)-ouabain-I/R group (PP2-OUA-I/R). The isolated rat hearts were perfused on the Langendorff apparatus. Except for NC group, all the hearts were subjected to 30 min global ischemia and followed by 120 min reperfusion. The cardiac function indexes were recorded at the same time. The coronary effluent was collected for estimating the activity of lactate dehydrogenase (LDH) and creatine kinase (CK). The activity of Na-K-ATPase and intracellular calcium concentration in myocardial tissue were measured. Apoptosis was evaluated by Flow cytometric analysis. The expressions of sodium pump α1 subunit, glucose regulated protein(GRP78),C/EBP homologous protein (CHOP) and Bcl-2/Bax were determined by Western blot.

Results: Pretreatment with ouabain significantly reduced the recovery of cardiac function, increased the levels of CK, LDH and intracellular calcium concentration, decreased the activity of Na-K-ATPase. In addition, ouabain markedly increased the myocardial apoptosis index, down-regulated the expressions of sodium pump α1 subunit and Bcl-2, up-regulated the expressions of GRP78,CHOP and Bax; while these changes were significantly improved in the Anti-Dig-I/R group compared with those in the I/R group; PP2 or U73122 partially blocked the effects of ouabain on myocardial I/R injury. Compared with the OUA-IR group, the recovery of cardiac function, the activity of Na-K-ATPase and the expressions of sodium pump α subunit and Bcl-2 were significant higher, meanwhile the leakage of CK and LDH, intracellular calcium concentration, myocardial apoptosis index and the expressions of GRP78 and Bax were significantly lower in PP2-OUA-I/R and U73122-OUA-I/R group.

Conclusions: Changes in sodium pump function and endoplasmic reticulum stress all participate in the process of I/R injury. Current findings further suggest that sodium pump mediates ERS by activating signals of Src and PLC pathway, which may be one of the mechanisms of apoptosis induced by I/R injury.
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http://dx.doi.org/10.12047/j.cjap.5448.2017.010DOI Listing
January 2017

[Regulation Mechanism of Ginkgo-Dipyridamolum for Calcium Homeostasis on Cardioprotective Effect During Ischemia Reperfusion Injury].

Zhong Yao Cai 2015 Dec;38(12):2557-62

Objective: To explore regulatory mechanism of Ginkgo-dipyridamolum (GD) for calcium homeostasis on cardioprotective effect during ischemia reperfusion injury in the isolated rat heart.

Methods: 40 male SD-rats were randomly divided into five groups (n = 8): normal control group (NC), ischemia reperfusion group (IR), GD precondition group (GD + IR), Nicardipine and GD precondition group( Nic + GD + IR), and LaCl3 and GD precondition group (LaCl, + GD +IR). The hearts of rats were isolated after anesthesia and performed to profuse with Langendorff equipment. The heart functional indexes (HR, LVSP and ± dp/dt(max)) were detected at the five time points (stabilize point, ischemia 30 min, reperfusion 5 min, reperfusion 30 min, and reperfusion 60 min). The coronary effluents were also collected at the five time points. The activities of LDH and CK were measured, respectively, as well as the Ca2+ contents. After the experiments were finished,the myocardial mitochondria were isolated, homogenated and then the Ca2+ concentrations, the activities of IDH and α-OGDH were detected. The pathologic changes in myocardial tissues were also observed by histologic section.

Results: Compared with IR group, the heart functional indexes ( LVSP x HR and ± dp/dt(max)) of GD + IR group were improved at the five time points; the enzymes (LDH and CK) release, the Ca2+ concentrations, the activities of IDH and α-OGDH were reduced in mitochondrion. However, the protective effects above could be inhibited by Nic and LaCl3. Histologic sections showed that the myocardial tissue in IR group was damaged obviously, the damaged myocardial tissues were repaired in GD + IR, Nic + GD + IR and LaCl, + GD + IR) groups, especially in GD + IR group.

Conclusion: Ginkgo-dipyridamolum can alleviate the myocardial ischemia reperfusion injury, the mechanism is probobaly related to maintaining calcium homeostasis and mitochondrial energy metabolism function.
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December 2015

[The mechanism of anthocyanin to anti-inflammatory and analgesic frnm Ligustrum fruit].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2015 Sep;31(5):431-6

Objective: To provide a theoretical basis for the further development of new drugs, the analgesic and anti-inflammatory effects and the liver function in mice of anthocyanin from Ligustrum vicaryi were investigated.

Methods: The 240 experimental mice were splitted equally for 6 kinds of experiments and 40 rats in each kind of experiment were divided into 5 groups (n = 8): normal saline control group (NS); aspirin control group (Asp); high-concentration anthocyanin group (HA); medium-concentration anthocyanin group (MA); low-concentratior anthocyanin group (LA). The analgesia effect of anthocyanin at different concentration was detenmined by hot-plate test and acetic acid writhin test, and the anti-inflammatory effect of anthocyanin was performed by ear edema, ahdomen capillary permeability and cotton granuloma. The activities of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) and the contents of nitric oxide (NO) and prostaglandin E2 (PGE2) in blood serum were determined, and the activities of SOD, T-AOC and glutathione peroxidase (GSH-Px) in liver were measured, while the histological changes of liver tissue were observed.

Results: The pain threshold of mice was enhanced and the times of twist body wa decreased by medium-concentration and high-concentration anthocyanin. The activity of SOD was increased and the conentrs of NO and PGE were reduced in blood serum. High-concentration anthocyanin inhibited the ear swelling , the increase of celiac capillary permeability and th granuloma hyperplasia, and increased the activities of SOD and T-AOC while decreased the content of PGE2. The activities of SOD, T-AOC. GSH-PX were increased in liver, but the morphology of liver tissues in each group had no significant changes.

Conclusion: Anthocyanin Ligustrum vicaryi has definite anti-inflammatory and analgesic effects, which is related to increasing the antioxidant capacity and decreasing th contents of NO and PGE2, and has not obvious damage to liver in the range of experimental concentration.
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September 2015

Image routing via atomic spin coherence.

Sci Rep 2015 Dec 10;5:18179. Epub 2015 Dec 10.

Key Laboratory of Coherence Light, Atomic and Molecular Spectroscopy, College of Physics, Jilin University, Changchun, 130012, P. R. China.

Coherent storage of optical image in a coherently-driven medium is a promising method with possible applications in many fields. In this work, we experimentally report a controllable spatial-frequency routing of image via atomic spin coherence in a solid-state medium driven by electromagnetically induced transparency (EIT). Under the EIT-based light-storage regime, a transverse spatial image carried by the probe field is stored into atomic spin coherence. By manipulating the frequency and spatial propagation direction of the read control field, the stored image is transferred into a new spatial-frequency channel. When two read control fields are used to retrieve the stored information, the image information is converted into a superposition of two spatial-frequency modes. Through this technique, the image is manipulated coherently and all-optically in a controlled fashion.
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http://dx.doi.org/10.1038/srep18179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4674752PMC
December 2015

A Cationic MOF with High Uptake and Selectivity for CO2 due to Multiple CO2 -Philic Sites.

Chemistry 2015 Nov 29;21(46):16525-31. Epub 2015 Sep 29.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, of the Ministry of Education, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069 (P. R. China).

The reaction of N-rich pyrazinyl triazolyl carboxyl ligand 3-(4-carboxylbenzene)-5-(2-pyrazinyl)-1H-1,2,4-triazole (H2 cbptz) with MnCl2 afforded 3D cationic metal-organic framework (MOF) [Mn2 (Hcbptz)2 (Cl)(H2 O)]Cl⋅DMF⋅0.5 CH3 CN (1), which has an unusual (3,4)-connected 3,4T1 topology and 1D channels composed of cavities. MOF 1 has a very polar framework that contains exposed metal sites, uncoordinated N atoms, narrow channels, and Cl(-) basic sites, which lead to not only high CO2 uptake, but also remarkably selective adsorption of CO2 over N2 and CH4 at 298-333 K. The multiple CO2 -philic sites were identified by grand canonical Monte Carlo simulations. Moreover, 1 shows excellent stability in natural air environment. These advantages make 1 a very promising candidate in post-combustion CO2 capture, natural-gas upgrading, and landfill gas-purification processes.
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http://dx.doi.org/10.1002/chem.201502532DOI Listing
November 2015

[Effects of total flavonoids of propolis on apoptosis of myocardial cells of chronic heart failure and its possible mechanism in rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2015 May;31(3):201-6

Objective: To investigate the effects of total flavonoids of propolis (TFP) on apoptosis of myocardial cells of chronic heart failure and its possible mechanism in rats.

Methods: Six male SD rats were randomly selected as normal control group, the remaining rats were made as chronic heart failure (CHF) model by intraperitoneal injection of adriamycin. The rats in the successful model were randomly divided into five groups (n = 6): CHF group, total flavonoids of propolis low dose group (LD group), total flavonoids of propolis middle dose group (MD group), total flavonoids of propolis high dose group (HD group), digoxin group (DIG group). After six week treatment, cardiac function indexes of rats were recorded by signal acquisition system; brain natriuretic peptide (BNP), cardiac troponin I (cTnI), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) content in plasma were detected; Myocardial morphological changes and collagen fiber hyperplasia by HE and Masson staining were observed; Myocardial apoptosis was detected with TUNEL method and protein connexin 43(P-Cx43) expression was detected by Western blot method.

Results: Compared with NC group, left ventricular systolic pressure(LVSP) and maximal rise/fall velocity of left ventriculad pressure (± dP/dt(max)) absolute value in CHF group were significantly lowered (P < 0.01) while left ventricular end diastolic pressure (LVEDP) was increased significantly (P < 0.01); Contents of plasma BNP, cTnI, TNF-α and IL-6 in the CHF group were significantly improved (P < 0.01). Compared with CHF group, LVSP, ± dP/dt(max) absolute value in MD and HD groups were increased (P < 0.05), and LVEDP was significantly lowered (P < 0.01); LVEDP in LD group was significantly lowered (P < 0.01), changes in LVSP and ± dp/dt(max) absolue value were not obvious (P > 0.05). BNP, cTnI, TNF-α and IL-6 contents in MD and HD groups were significantly reduced (P < 0.01), but those plasma indicator changes were not obvious in LD group (P > 0.05). Western blot showed that P-Cx43 expression in CHF group was significantly higher than that in NC group (P < 0.01) and that in all TFP treatment groups it was decreased compared with CHF group (P < 0.05, P < 0.01), among which pairwise comparisons also showed differences (P < 0.05), myocardial apoptosis index (%)(22.62 ± 3.39) in CHF group was higher than that in NC group( 1.12 ± 0.24) (P < 0.01); compared with CHF group, the apoptosis index of myocardial cells (%) in LD,MD and HD groups, (15.79 + 2.8), (9.28 + 2.1) and (4.73 + 1.14) respectively, were significantly lower than those in the CHF group( P < 0.01). The expression level of P-Cx43 positively correlated with the apoptotic index (r = 0. 861, P < 0.01).

Conclusion: Total flavonaids of propolis have inhibitory effect on apoptosis of myocardial cells of chronic heart failure induced by adriamycin in rats, and the mechanism may be closely related to the regulation of Cx43 expression, especially the regulatory phosphorylation status.
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May 2015

[The effect of acid etching on bond strength of different self-adhesive resin cements to dentin].

Shanghai Kou Qiang Yi Xue 2015 Jun;24(3):302-6

Department of Stomatology, Shaoxing People's Hospital. Shaoxing 312000, Zhejiang Province,

Purpose: To investigate the effect of acid etching on bond strength (μTBS) of self-adhesive resin cements (Unicem, G-Cem, Clearfil SA Cement, BisCem) to dentin.

Methods: Thirty-two human third molars without caries were used for the study. One fourth of the crowns were cut off, perpendicular to the long axis of the tooth with (Isomet) a low-speed saw, in order to expose the dentin surfaces of the sectioned sides. The sectioned dentin surfaces of the experimental groups were etched for 15 s. Subsequently, resin blocks were bonded to the dentin surfaces, with one of the four cements (Unicem, G-Cem, Clearfil SA Cement, BisCem). After being stored in water for 24 hours, all specimens were perpendicularly sectioned through the dentin-resin interfaces, prepared into beams about 1 mm×1 mm×8 mm for μTBS tests with micro-tensile tester. The micro-morphologies of the cement-dentin interfaces were assessed using scanning electron microscope. The data was analyzed using SPSS16.0 software package.

Results: Without pre-treatment of acid etching, Unicem group (12.9±3.2) MPa, G-Cem group (11.7±2.6) MPa and Clearfil SA Cement group (10.9±2.3) MPa possessed a higher bond strength than BisCem group (6.8±2.4) MPa (P<0.05). Specimens with pre-treatment of acid etching showed a significantly lower dentin bond strength compared to those without pretreatment of acid etching (P<0.05) in self-adhesive cements (G-Cem, Clearfil SA CementUnicem, Unicem), but there was no significant difference in bond strength in BisCem group with or without acid etching.

Conclusions: With pretreatment of acid etching, the bond strength of self-adhesive resin cements to dentin decreases in G-Cem, Clearfil SA Cement and Unicem group, while there is no significant effect on the bond strength of BisCem group. Supported by Public Technology Applied Research Project of Science and Technology Bureau of Shaoxing City of Zhejiang Province (2012B70079).
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June 2015

A new porous MOF with two uncommon metal-carboxylate-pyrazolate clusters and high CO2/N2 selectivity.

Inorg Chem 2015 Feb 30;54(4):1841-6. Epub 2015 Jan 30.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an 710127, P. R. China.

By a less-exploited strategy, a stable framework was constructed by using 4,4'-biphenyldicarboxylic acid (H2bpdc) and methyl-functionalized 3,3',5,5'-tetramethyl-4,4'-bipyrazole (H2bpz) coligands, revealing a new (6,8)-connected net based on two extremely rare trinuclear and tetranuclear metal-carboxylate-pyrazolate clusters. The framework is very porous and possesses not only high CO2 loadings but also very high CO2/N2 selectivities at 308 and 313 K because of the polar pore surface decorated by clusters, pyrazolyl units, and confined cages with methyl groups dangling. Importantly, GCMC simulation identified two favorable CO2 sorption sites located sequentially near Co3(pz)3 and Co3(CO2)2(pz) motifs of the tetranuclear cluster, and the multipoint framework-CO2 interactions were distinguished. The framework also displays remarkable stability toward water and organic solvents.
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http://dx.doi.org/10.1021/ic502733vDOI Listing
February 2015

Pseudomonas hunanensis sp. nov., isolated from soil subjected to long-term manganese pollution.

Curr Microbiol 2014 Jul 23;69(1):19-24. Epub 2014 Feb 23.

School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, China,

A Gram-negative, polar flagella, rod-shaped bacterium LV (T) was isolated from a soil sample subjected to long-term manganese pollution in Hunan Province, China. Cells grow optimally on Luria-Bertani agar medium at 30 °C in the presence of 0-5.0 % (w/v) NaCl and pH 7-8. 16S rRNA gene sequence analysis revealed that strain LV (T) belonged to the genus Pseudomonas, with sequence similarity values of 98.6, 98.2, 98.7, and 97.3 % to Pseudomonas monteilii BCRC 17520 (T) , Pseudomonas putida BCRC 10459 (T) , Pseudomonas plecoglossicida BCRC 17517 (T) , and Pseudomonas asplenii BCRC 17131 (T) , respectively. The level of DNA-DNA relatedness between the five strains was <30 %. The DNA G+C content of strain LV (T) is 68.8 mol%. Chemotaxonomic data revealed that the strain LV(T) possesses ubiquinone Q-9. The polar lipid profile of strain LV (T) contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine. The major cellular fatty acids present are C10:03-OH (12.33 %), C16:0 (23.99 %), summed feature 3(C16:1ω7c and/or C16:1ω6c), and summed feature 8(C18:1 ω7c and C18:1 ω6c). Based on the genotypic, chemotaxonomic and phenotypic data, strain LV (T) is distinguishable from related members of the genus Pseudomonas. Thus, strain LV (T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas hunanensis sp. nov. is proposed. The type strain is LV (T) (=CICC 10558(T) = NCCB 100446(T)).
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http://dx.doi.org/10.1007/s00284-014-0545-4DOI Listing
July 2014

[Detection of onion soluble solids content based on the near-infrared reflectance spectra].

Guang Pu Xue Yu Guang Pu Fen Xi 2013 Sep;33(9):2403-6

Key Laboratory on Modern Precision Agriculture System Integration Research of MOE, China Agricultural University, Beijing 100083, China.

Onion soluble solids content (SSC) was detected using near-infrared (924-1720 nm) reflectance spectra. Three cultivars of onions, harvested at different period, were selected for experiment and the total number of samples is 268. SSC reference value of onion juice was determined using the temperature compensated refractometer. Some pre-processing methods, such as S-G smoothing, scatter correction, and derivation, were compared to establish a statistical model based on partial least squares regression (PLSR) method. The results show that the avitzky-Golay smoothing with window 32 and span 10 is more efficient. The determination correlation coefficient of prediction R2 is 0.87 and root mean square error (RMSEP) is 2.42 degrees Brix. Compared to the 2nd derivation, the 1st derivation got better prediction result, but the spectra scatter correction is the best (R2 = 0.88, RMSEP of = 2.31 degrees Brix). The optimal prediction (R2 = 0.90, RMSEP = 1.84 degrees Brix and RPD = 3) was built based on crossing validation modeling, which shows that infrared reflectance spectroscopy with scatter correction pre-processing is feasible for onions soluble solids detection.
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September 2013

[Multiple scattering of visible and infrared light by sea fog over wind driving rough sea surface].

Guang Pu Xue Yu Guang Pu Fen Xi 2013 Aug;33(8):2075-8

School of Electrical and Electronic Engineering, Shandong University of Technology, Zibo 255049, China.

The present paper is concerned with computing the multiple scattering characteristics of a sea fog-sea surface couple system within this context. The single scattering characteristics of sea fog were studied by Mie theory, and the multiple scattering of sunlight by single sea fog layer was studied by radiative transfer theory. The reflection function of a statistically rough ocean surface was obtained using the standard Kirchhoff formulation, with shadowing effects taken into account. The reflection properties of the combined sea fog and ocean surface were obtained employing the adding method, and the results indicated that the reflected light intensity of sea fog increased with the sea background.
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August 2013
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