Publications by authors named "Hai-bo WANG"

273 Publications

Using random forest to detect multiple inherited metabolic diseases simultaneously based on GC-MS urinary metabolomics.

Talanta 2021 Dec 19;235:122720. Epub 2021 Jul 19.

Yuncheng Maternal and Child Health Hospital, Yuncheng, Shanxi, 044000, PR China.

Inborn errors of metabolism, also known as inherited metabolic diseases (IMDs), are related to genetic mutations and cause corresponding biochemical metabolic disorder of newborns and even sudden infant death. Timely detection and diagnosis of IMDs are of great significance for improving survival of newborns. Here we propose a strategy for simultaneously detecting six types of IMDs via combining GC-MS technique with the random forest algorithm (RF). Clinical urine samples from IMD and healthy patients are analyzed using GC-MS for acquiring metabolomics data. Then, the RF model is established as a multi-classification tool for the GC-MS data. Compared with the models built by artificial neural network and support vector machine, the results demonstrated the RF model has superior performance of high specificity, sensitivity, precision, accuracy, and matthews correlation coefficients on identifying all six types of IMDs and normal samples. The proposed strategy can afford a useful method for reliable and effective identification of multiple IMDs in clinical diagnosis.
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http://dx.doi.org/10.1016/j.talanta.2021.122720DOI Listing
December 2021

Effects of Decompressive Cervical Surgery on Blood Pressure in Cervical Spondylosis Patients With Hypertension: A Time Series Cohort Study.

Int J Spine Surg 2021 Aug 15;15(4):683-691. Epub 2021 Jul 15.

Peking University Clinical Research Institute, Peking University First Hospital, Beijing, China.

Background: The blood pressure of cervical spondylosis (CS) patients with hypertension often returns to normal after decompressive cervical surgery (DCS). However, the effect of DCS on the blood pressure of patients with CS has not been rigorously studied.

Methods: We recruited 50 consecutive CS patients with hypertension from 2014-2017 and investigated the changes in blood pressure after DCS using a time series design. Ambulatory blood pressure monitoring (ABPM) was performed at 3 and 0 days before DCS and at 30 and 90 days after DCS. The primary outcome was mean 24-hour systolic blood pressure (SBP). Secondary outcomes included mean 24-hour diastolic blood pressure (DBP), office blood pressure, and the percentage of patients on antihypertensive medication. Paired test was used for assessing the changes in blood pressure over time and a McNemar test was used for comparison among different medication groups.

Results: The mean 24-hour SBP did not vary significantly among 4 time points (134.5 ± 14.7, 132.8 ± 14.7, 131.5 ± 13.3, and 133.2 ± 14.6, respectively; = .42). The mean 24-hour DBP showed a similar trend. However, mean office SBP/DBP decreased significantly from 142.5/82.0 mm Hg before surgery to 127.3/76.6 mm Hg after surgery (both < .01). The corresponding percentage of patients on antihypertensive medication decreased significantly, from 84% to 54% ( < .01).

Conclusions: This study confirmed previous findings of reduction in office blood pressure associated with DCS among CS patients with hypertension. However, this was not confirmed by multiple-time series of 24-hour ABPM.

Level Of Evidence: 3.
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http://dx.doi.org/10.14444/8090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375702PMC
August 2021

L-Histidine-DNA interaction: a strategy for the improvement of the fluorescence signal of poly(adenine) DNA-templated gold nanoclusters.

Mikrochim Acta 2021 05 26;188(6):198. Epub 2021 May 26.

College of Chemistry and Chemical Engineering, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People's Republic of China.

An interesting phenomenon is described that the fluorescence signal of poly(adenine) (A) DNA-templated gold nanoclusters (AuNCs) is greatly improved in the presence of L-histidine by means of L-histidine-DNA interaction. The modified nanoclusters display strong fluorescence emission with excitation/emission maxima at 290/475 nm. The fluorescence quantum yield (QY) is improved from 1.9 to 6.5%. Fluorescence enhancement is mainly ascribed to the L-histidine-DNA interaction leading to conformational changes of the poly(A) DNA template, which offer a better microenvironment to protect AuNCs. The assay enables L-histidine to be determined with good sensitivity and a linear response that covers the 1 to 50 nM L-histidine concentration range with a 0.3 nM limit of detection. The proposed method has been applied to the determination of imidazole-containing drugs in pharmaceutical samples. A turn-on fluorescent method has been designed for the sensitive detection of L-histidine as well as imidazole-containing drugs on the basis of the L-histidine-DNA interaction.
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http://dx.doi.org/10.1007/s00604-021-04853-7DOI Listing
May 2021

A comparison of non-intubated video-assisted thoracic surgery with spontaneous ventilation and intubated video-assisted thoracic surgery: a meta-analysis based on 14 randomized controlled trials.

J Thorac Dis 2021 Mar;13(3):1624-1640

Department of Thoracic Surgery, Affiliated Hospital of Hebei University, Baoding, China.

Background: Video-assisted thoracic surgery (VATS) generally involves endotracheal intubation under general anesthesia. However, inevitably, this may cause intubation-related complications and prolong the postoperative recovery process. Gradually, non-intubated video-assisted thoracic surgery (NIVATS) is increasingly being utilized. However, its safety and efficacy remain controversial.

Methods: Randomized controlled trials (RCTs) published up to August 2020 were selected from the Cochrane Library, Web of Science, PubMed, Embase, and ClinicalTrials.gov databases and included in this study according to the inclusion criteria. Two reviewers screened these RCTs and independently extracted the relevant data. After assessing the risk of bias in these RCTs, a meta-analysis was performed using Review Manager 5.3. Pooled data were meta-analyzed using a random-effects model.

Results: Meta-analysis data demonstrated that the mean difference (MD) in the length of hospital stay between non-intubated patients and intubated patients was -1.41 days, with a 95% confidence interval (CI) of -2.47 to -0.34 (P=0.01). The visual analogue scale (VAS) score between the two groups showed a MD of -0.34 (95% CI: -0.58 to -0.10; P=0.006). Patients who underwent NIVATS presented with lower rates of overall complications [odds ratio (OR) 0.41; 95% CI: 0.25 to 0.67; P=0.0004], air leak (OR 0.45; 95% CI: 0.24 to 0.87; P=0.02), pharyngeal discomfort (OR 0.08; 95% CI: 0.04 to 0.17; P<0.00001), hoarseness (OR 0.06; 95% CI: 0.02 to 0.21; P<0.00001), and gastrointestinal reactions (OR 0.23; 95% CI: 0.10 to 0.53; P=0.0005) compared to intubated patients. The anesthesia satisfaction scores in the NIVATS group were significantly higher than those of the VATS group (MD 0.50; 95% CI: 0.12 to 0.88; P=0.009). However, there were no statistically significant differences in the length of operation time (MD 0.90 hours; 95% CI: -0.23 to 2.03; P=0.12) and surgical field satisfaction (1 point) (OR 0.73; 95% CI: 0.34 to 1.59; P=0.43) between the two groups.

Conclusions: NIVATS is a safe and feasible form of intervention that can reduce the postoperative pain and complications of various systems and shorten hospital stay duration without prolonging the operation time.
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http://dx.doi.org/10.21037/jtd-20-3039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024812PMC
March 2021

Associations Between Elevated Systolic Blood Pressure and Outcomes in Critically Ill Patients: A Retrospective Cohort Study and Propensity Analysis.

Shock 2021 10;56(4):557-563

First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China.

Introduction: Studies have shown nonlinear relationships between systolic blood pressure (SBP) and outcomes, with increased risk observed at both low and high blood pressure levels. However, the relationships between cumulative times at different SBP levels and outcomes in critically ill patients remain unclear. We hypothesized that an appropriate SBP level is associated with a decrease in adverse outcomes after intensive care unit (ICU) admission.

Methods: This study was a retrospective analysis of data from the Medical Information Mart for Intensive Care (MIMIC) III database, which includes more than 1,000,000 SBP records from 12,820 patients. Associations of cumulative times at four SBP ranges (<100 mm Hg, 100-120 mm Hg, 120-140 mm Hg, and ≥140 mm Hg) with mortality (12-, 3-, 1-month mortality and in-hospital mortality) were evaluated. Restricted cubic splines and multivariable Cox regression models were employed to assess associations between mortality and cumulative times at SBP levels (4 levels: <2, 2-12, 12-36, and ≥36 h) over 72 h of ICU admission. Additionally, 120 mm Hg to 140 mm Hg was subdivided into <12 h (Group L) and ≥12 h (Group M) subsets and subjected to propensity-score matching and subgroup analyses.

Results: At 120 mm Hg to 140 mm Hg, level-4 SBP was associated with lower adjusted risks of mortality at 12 months (OR, 0.71; CI, 0.61-0.81), 3 months (OR, 0.72; CI, 0.61-0.85), and 1 month (OR, 0.61; CI, 0.48-0.79) and in the hospital (OR, 0.71; CI, 0.58-0.88) than level-1 SBP. The cumulative times at the other 3 SBP ranges (<100 mm Hg, 100-120 mm Hg, and ≥140 mm Hg) were not independent risk predictors of prognosis. Furthermore, Group M had lower 12-month mortality than Group L, which remained in the propensity-score matched and subgroup analyses.

Conclusions: SBP at 120 mm Hg to 140 mm Hg was associated with decreased adverse outcomes. Randomized trials are required to determine whether the outcomes in critically ill patients improve with early maintenance of a SBP level at 120 mm Hg to 140 mm Hg.
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http://dx.doi.org/10.1097/SHK.0000000000001774DOI Listing
October 2021

XRCC5/6 polymorphisms and their interactions with smoking, alcohol consumption, and sleep satisfaction in breast cancer risk: A Chinese multi-center study.

Cancer Med 2021 04 18;10(8):2752-2762. Epub 2021 Mar 18.

Department of Breast Surgery, Yantai Yuhuangding Hospital, Yantai, China.

Background: X-ray repair cross-complementary 5 (XRCC5) and 6 (XRCC6) are critical for DNA repair. Few studies have assessed their association with breast cancer risk, and related gene-environment interactions remain poorly understood. This study aimed to determine the influence of XRCC5/6 polymorphisms on breast cancer risk, and their interactions with cigarette smoking, alcohol consumption, and sleep satisfaction.

Methods: The study included 1039 patients with breast cancer and 1040 controls. Four single-nucleotide polymorphisms of XRCC5 and two of XRCC6 were genotyped. Information about smoking, alcohol consumption, and sleep satisfaction was collected through questionnaires. Odds ratios (OR) and related 95% confidence intervals (95% CI) were assessed using unconditional logistic regression models. Gene-environment interactions were analyzed using logistic regression with multiplicative interaction models.

Results: XRCC5 rs16855458 was associated with increased breast cancer risk in the co-dominant (p  = 0.003) and dominant (CA + AA vs. CC, OR = 1.29, 95% CI = 1.07-1.56, p = 0.008) genetic models after Bonferroni correction. The CG + GG genotype of XRCC6 rs2267437 was associated with an increased risk of estrogen receptor-negative/progesterone receptor-negative (ER-/PR-) breast cancer (CG + GG vs. CC: OR = 1.54, 95% CI = 1.12-2.13, p = 0.008) after Bonferroni correction. Moreover, an antagonistic interaction between XRCC5 rs16855458 and alcohol consumption (p  = 0.017), and a synergistic interaction between XRCC6 rs2267437 and sleep satisfaction were associated with breast cancer risk (p  = 0.0497). However, these interactions became insignificant after Bonferroni correction.

Conclusion: XRCC5 rs16855458 was associated with breast cancer risk, and XRCC6 rs2267437 was associated with the risk of ER-/PR- breast cancer. Breast cancer risk associated with XRCC5 and XRCC6 polymorphisms might vary according to alcohol consumption and sleep satisfaction, respectively, and merit further investigation.
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http://dx.doi.org/10.1002/cam4.3847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026916PMC
April 2021

Preventing forgery attacks in computational ghost imaging or disabling ghost imaging in a "spatiotemporal" scattering medium with weighted multiplicative signals.

Appl Opt 2021 Feb;60(5):1092-1098

The ghost imaging (GI) approach is an intriguing and promising image acquisition technique that can transmit high-quality image information in a scattering environment. In this paper, we focus on two concerns recently emerged in the GI modality: one is the vulnerability to forgery attacks in GI-based optical encryption [Opt. Lett.45, 3917 (2020)OPLEDP0146-959210.1364/OL.392424], and the other is the potential threat of GI to personal privacy regarding non-invasive imaging [Opt. Express28, 17232 (2020)OPEXFF1094-408710.1364/OE.391788]. The core idea is to recommend introducing weighted multiplicative signals [Opt. Express27, 36505 (2019)OPEXFF1094-408710.1364/OE.27.036505] into the computational GI system, whether on the transmitting end or the receiving end. At the transmitting end, the random multiplicative signal can be used as an additional key that can reduce the possibility of forgery attacks, thereby increasing image transmission security. On the receiving end, the introduction of a random multiplicative signal to a spatial scattering medium makes it a "spatiotemporal" scattering medium, whose transmittance changes with time. Further, the spatiotemporal scattering medium can disable direct imaging and GI at the same time with low cost, thereby having great potential in privacy protection in daily lives.
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http://dx.doi.org/10.1364/AO.415608DOI Listing
February 2021

Chronic Respiratory Diseases and the Outcomes of COVID-19: A Nationwide Retrospective Cohort Study of 39,420 Cases.

J Allergy Clin Immunol Pract 2021 07 6;9(7):2645-2655.e14. Epub 2021 Mar 6.

State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

Background: Chronic respiratory diseases (CRD) are common among patients with coronavirus disease 2019 (COVID-19).

Objectives: We sought to determine the association between CRD (including disease overlap) and the clinical outcomes of COVID-19.

Methods: Data of diagnoses, comorbidities, medications, laboratory results, and clinical outcomes were extracted from the national COVID-19 reporting system. CRD was diagnosed based on International Classification of Diseases-10 codes. The primary endpoint was the composite outcome of needing invasive ventilation, admission to intensive care unit, or death within 30 days after hospitalization. The secondary endpoint was death within 30 days after hospitalization.

Results: We included 39,420 laboratory-confirmed patients from the electronic medical records as of May 6, 2020. Any CRD and CRD overlap was present in 2.8% and 0.2% of patients, respectively. Chronic obstructive pulmonary disease (COPD) was most common (56.6%), followed by bronchiectasis (27.9%) and asthma (21.7%). COPD-bronchiectasis overlap was the most common combination (50.7%), followed by COPD-asthma (36.2%) and asthma-bronchiectasis overlap (15.9%). After adjustment for age, sex, and other systemic comorbidities, patients with COPD (odds ratio [OR]: 1.71, 95% confidence interval [CI]: 1.44-2.03) and asthma (OR: 1.45, 95% CI: 1.05-1.98), but not bronchiectasis, were more likely to reach to the composite endpoint compared with those without at day 30 after hospitalization. Patients with CRD were not associated with a greater likelihood of dying from COVID-19 compared with those without. Patients with CRD overlap did not have a greater risk of reaching the composite endpoint compared with those without.

Conclusion: CRD was associated with the risk of reaching the composite endpoint, but not death, of COVID-19.
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http://dx.doi.org/10.1016/j.jaip.2021.02.041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935669PMC
July 2021

The effect and mechanism of miR-607/CANT1 axis in lung squamous carcinoma.

Anticancer Drugs 2021 08;32(7):693-702

Department of Nuclear Medicine, Central Hospital of Shan County, Shan County, Heze, Shandong People's Republic of China.

Lung squamous carcinoma (LUSC) is the second most frequent subtype of non-small cell lung cancer. Rarely gene alterations are identified in LUSC. Therefore, identifying LUSC-related genes to explain the relevant molecular mechanism is urgently needed. A potential biomarker, calcium-activated nucleotidase 1 (CANT1), was elevated in tissues of LUSC patients relative to normal cases based on the TCGA and/or GTEx database. CCK-8 and transwell tests were then implemented to measure the proliferative, invasive and migratory capacities, and showed that knockdown of CANT1 blocked LUSC cells proliferation. miR-607, predicted as an upstream factor for CANT1, was declined in LUSC using TargetScan analysis and luciferase activity test. Low miR-607 expression was related with unfavorable outcomes of LUSC patients. Moreover, miR-607 downregulation elevated cell viability, invasion and migration in LUSC cells, which was antagonized by si-CANT1. GEPIA website was accessed to estimate the relevance between CANT1 and epithelial-mesenchymal transition (EMT)-related positive factors. The protein levels of Fibronectin, Vimentin, Snail and β-catenin were altered due to the abnormal CANT1 and miR-607 expression. Together, these data unveiled that miR-607/CANT1 pair may exert a vital role in the progression of LUSC through mediating EMT process, which would furnish an available therapeutic therapy for LUSC.
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http://dx.doi.org/10.1097/CAD.0000000000001045DOI Listing
August 2021

Quantitative analysis of various targets based on aptamer and functionalized [email protected] oxide in dairy products using pregnancy test strip and smartphone.

Food Chem 2021 Aug 20;352:129330. Epub 2021 Feb 20.

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, PR China; Key Laboratory of Biomedical Photonics (HUST), Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, Hubei, PR China. Electronic address:

Pregnancy test strips are one of the most mature and widely used commercial lateral flow devices used to determine pregnancy. Being a simple and rapid detection method, human chorionic gonadotropin (hCG) was used with different aptamers (hCG-apt) as probes for the detection of metal ions, small organic molecules, and proteins. Quantitative detection of target analytes was achieved using a smartphone app and a portable device developed in our laboratory. The results showed detection ranges of 1 nM-1 μM, 0.1 nM-10 μM and 32 nM-500 nM for Pb, chloramphenicol, and β-lactoglobulin, respectively, and the corresponding visual detection limits in dairy products were 5 nM, 1 nM and 50 nM, respectively. Based on these results, rapid detection of multiple analytes can be realized through aptamer modification, thereby broadening the application range of commercial lateral flow devices for analysis of food chemistry.
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http://dx.doi.org/10.1016/j.foodchem.2021.129330DOI Listing
August 2021

Dynamic Tensile Properties and Energy Dissipation of High-Strength Concrete after Exposure to Elevated Temperatures.

Materials (Basel) 2020 Nov 24;13(23). Epub 2020 Nov 24.

State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Mine, Anhui University of Science &Technology, Huainan 232001, China.

In view of the devastating outcomes of fires and explosions, it is imperative to research the dynamic responses of concrete structures at high temperatures. For this purpose, the effects of the strain rate and high temperatures on the dynamic tension behavior and energy characteristics of high-strength concrete were investigated in this paper. Dynamic tests were conducted on high-strength concrete after exposure to the temperatures of 200, 400, and 600 °C by utilizing a 74 mm diameter split Hopkinson pressure bar (SHPB) apparatus. We found that the quasi-static and dynamic tensile strength of high-strength concrete gradually decreased and that the damage degree rose sharply with the rise of temperature. The dynamic tensile strength and specific energy absorption of high-strength concrete had a significant strain rate effect. The crack propagation law gradually changed from directly passing through the coarse aggregate to extending along the bonding surface between the coarse aggregate and the mortar matrix with the elevation of temperature. When designing the material ratio, materials with high-temperature resistance and high tensile strength should be added to strengthen the bond between the mortar and the aggregate and to change the failure mode of the structure to resist the softening effect of temperature.
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http://dx.doi.org/10.3390/ma13235313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727677PMC
November 2020

Promising Rice-Husk-Derived Carbon/Ni(OH) Composite Materials as a High-Performing Supercapacitor Electrode.

ACS Omega 2020 Nov 11;5(46):29896-29902. Epub 2020 Nov 11.

Province Key Laboratory of Cereal Resource Transformation and Utilization, Henan University of Technology, Zhengzhou, Henan 450001, China.

Improving the electrochemical performance of biomass-derived carbon electrode-active materials for supercapacitor applications has recently attracted considerable attention. Herein, we develop hybrid electrode materials from rice-husk-derived porous carbon (RH-C) materials and β-Ni(OH) via a facile solid-state reaction strategy comprising two steps. The prepared RH-C/Ni(OH) (C-Ni) was investigated using scanning electron microscopy (SEM) (energy-dispersive X-ray spectrometer (EDS)), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) to acquire the physical and chemical information, which was used to demonstrate the successful fabrication of C-Ni. Thermogravimetric analysis (TGA) measurement results confirmed that the thermal stability of C-Ni changed due to the presence of Ni(OH). As expected, C-Ni possesses a high capacitance of ∼952 F/g at a current density of 1.0 A/g. This result is higher than that of pure biomass-based carbon materials under the three-electrode system. This facile preparation method, which was used to synthesize the electrode-active materials, can extend to the value-added utility of other waste biomass materials as high-performing supercapacitor electrodes for energy storage applications.
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http://dx.doi.org/10.1021/acsomega.0c04117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689945PMC
November 2020

Comparison between computed tomography and magnetic resonance imaging in clinical diagnosis and treatment of tibial platform fractures.

World J Clin Cases 2020 Sep;8(18):4067-4074

Department of CT, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China.

Background: Tibial plateau fracture is one of the common fracture types. It occurs mainly in teenagers and is usually caused by a fall. After the occurrence of fracture, knee swelling, pain, limited activity, . greatly affect the patient's exercise habits and lifestyle. X-ray, computed tomography (CT) and magnetic resonance imaging (MRI) were used in this examination. X-rays are relatively new and easy to operate. However, there are some errors in the observation of fracture collapse and fracture displacement. In recent years, CT and MRI have been actively used to diagnose various types of clinical fractures. They have more diagnostic power than X-ray film. However, some scholars believe that CT is also prone to errors in clinical application. The volume effect leads to missed diagnosis and misdiagnosis in some cases, while the multidirection scanning of MRI technology can effectively overcome the shortcomings of CT. To facilitate the selection of clinical examination regimens, this study further observed the diagnostic ability of these two regimens in the diagnosis of tibial plateau fractures.

Aim: To explore the value of nuclear MRI and CT in the clinical diagnosis of tibial plateau fractures.

Methods: A total of 120 patients with tibial plateau fractures admitted from September 2017 to August 2019 were included. All patients were examined by nuclear MRI and CT scanning. The results were sent to senior physicians in our hospital to complete the diagnosis.

Results: Nuclear magnetic resonance showed the same effects as CT in four aspects: fracture displacement, bone defect, fracture site and fracture comminution. There was no significant difference in the score data ( > 0.05). Nuclear magnetic resonance and CT tended to be consistent in the B3, C2 and C3 fracture diagnosis coincidence rate, combined injury detection rate and fracture detection rate. The diagnostic coincidence rate of type B1, B2 and C1 fractures and the accuracy rate of overall fracture classification indicated that the MRI technique was significantly better than that of CT ( > 0.05).

Conclusion: MRI and CT have good diagnostic typing in the diagnosis of tibial plateau fractures, but MRI is more accurate and may be preferred.
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http://dx.doi.org/10.12998/wjcc.v8.i18.4067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520768PMC
September 2020

Development and Evaluation of an iiPCR Assay for and Detection on a Field-Deployable PCR System.

Can J Infect Dis Med Microbiol 2020 7;2020:9373984. Epub 2020 Sep 7.

State Key Laboratory of Diarrhea Disease Detection, Zhuhai International Travel Healthcare Center, Zhuhai 519020, Guangdong, China.

Background: and are often associated with fecal-oral transmission and cause large-scale outbreaks in centralized catering units and, therefore, should be frequently and strictly monitored, especially among food handlers. However, no specific and sensitive on-site detection method is available until now.

Methods: In this study, an insulated isothermal PCR assay for the detection of and on a field-deployable PCR system was developed. Specificity, sensitivity, reproducibility, and clinical accuracy of the assay were characterized and evaluated.

Results: The insulated isothermal PCR assay could be completed within 58 minutes with minimal pretreatment needed. The assay was specific and with good reproducibility. The limit of detection was 10 CFU/mL and 10 CFU/mL for and , respectively, which was comparable to multiplex real-time PCR. Mock on-site clinical evaluation results showed that the analytical sensitivity and specificity of the insulated isothermal PCR assay were 100% and 96.6%, while the positive predictive value and negative predictive value were 94.1% and 100%, respectively.

Conclusion: Based on our results, we believe that the assay developed herein could serve as an alternative method for preliminary screening and provide a valuable platform for the on-site detection of and , especially in resource-limited and developing countries.
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http://dx.doi.org/10.1155/2020/9373984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7492956PMC
September 2020

Urodynamic assessment of bladder storage function after radical hysterectomy for cervical cancer.

Chin Med J (Engl) 2020 Oct;133(19):2274-2280

Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing 100044, China.

Background: After radical hysterectomy for cervical cancer, the most common complication is lower urinary tract symptoms. Post-operatively, bladder capacity can alter bladder function for a prolonged period. This study aimed to identify factors affecting bladder storage function.

Methods: A multicenter, retrospective cohort study was conducted. Information of patients with stages IA2 to IIB cervical cancer with urodynamic study results were retrospectively collected from nine hospitals between June 2013 and June 2018 according to the inclusion criteria. Demographic, surgical, and oncological data were collected. The univariate and multivariate logistic regression was used to identify clinical factors associated with bladder storage function.

Results: Two hundred and three patients with cervical cancer had urodynamic testing post-operatively. Ninety-five (46.8%) patients were diagnosed with stress urinary incontinence (SUI). The incidence of low bladder compliance (LBC) was 23.2%. Twenty-seven (13.3%) patients showed detrusor overactivity (DO). Fifty-seven patients (28.1%) presented with a decreased maximum cystometric capacity (DMCC). The probability of composite bladder storage dysfunction was 68.0%. Multivariate analysis confirmed that laparoscopy represents a protective factor for SUI with an odds ratio of 0.498 (P = 0.034). Patients who underwent a nerve-sparing procedure were less odds to experience SUI (P = 0.014). A significant positive correlation between LBC and DO was observed (P < 0.001). A greater length of the resected vagina and chemoradiotherapy were common risk factors for LBC and DO, while radiotherapy exerted a stronger effect than chemotherapy. Additionally, patients who received chemoradiotherapy frequently developed a DMCC. The follow-up time was not correlated with bladder storage function.

Conclusion: A nerve-sparing procedure without longer resected vagina is recommended for protecting the bladder storage function.
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http://dx.doi.org/10.1097/CM9.0000000000001014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546844PMC
October 2020

A turn-on fluorescence strategy for cellular glutathione determination based on the aggregation-induced emission enhancement of self-assembled copper nanoclusters.

Analyst 2020 Oct;145(21):7009-7017

College of Chemistry and Chemical Engineering, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China.

As a class of ideal fluorescent nanomaterials, self-assembled copper nanoclusters (CuNCs) have attracted increasing interest. Unfortunately, most of these CuNCs only possessed bright luminescence in acidic solution, which limited their practical applications in a physiological environment. Retaining the strong fluorescence of these CuNCs in neutral or alkaline solution is still a challenging task. In this strategy, self-assembled CuNCs were prepared by using 4-methylthiophenol as the protecting ligand. The self-assembled CuNCs display stable and bright luminescence with excitation/emission maxima at 330/605 nm even in neutral and alkaline environments. Interestingly, with the addition of glutathione (GSH), the fluorescence intensity of CuNCs is enhanced strongly through the GSH-controlled aggregation-induced emission enhancement of self-assembled CuNCs. The turn-on fluorescence strategy can determine the GSH concentration in the range from 1 to 100 μM with a limit of detection of 300 nM. In addition, the method is employed for the determination of GSH levels in cells. Therefore, the turn-on fluorescence strategy is reliable, sensitive and suitable for the determination of cellular GSH levels.
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http://dx.doi.org/10.1039/d0an01247eDOI Listing
October 2020

Dimensional Changes of Lumbar Intervertebral Foramen in Direct Anterior Approach-Specific Hyperextension Supine Position.

Orthop Surg 2020 Aug 28;12(4):1173-1181. Epub 2020 Jun 28.

Department of Orthopaedic Surgery, The First Hospital of Jilin University, Changchun, China.

Objective: To investigate the changes in the lumbar intervertebral foramen (LIVF) dimensions from neutral supine to direct anterior approach (DAA)-specific hyperextension supine position through a standardized three-dimensional (3D) reconstruction computerized tomography (CT) method.

Methods: A total of 35 healthy volunteers (18 men and 17 women) were included in this retrospective study. The mean age of enrolled subjects was 28.9 ± 5.0 years. In September 2018, all the individuals underwent a 3D CT scan of the lumbar spine in neutral and 30° hyperextension supine positions, which mimicked the patient's position in DAA total hip arthroplasty (THA). The dimensions of the LIVF, including foraminal area, height, and width, were measured on 3D reconstructed CT models at all lumbar foraminal levels. Foraminal area was defined as the area bounded by the adjacent superior and inferior vertebral pedicles, the posterosuperior boundary of the inferior vertebral body, the surface of the intervertebral disc posteriorly, the posteroinferior boundary of the superior vertebral body, and the surface of the ligamentum flavum anteriorly. Foraminal height was defined as the longest distance between the border of the superior and the inferior pedicle. Foraminal width was defined as the shortest distance between the posteroinferior edge of the superior vertebrae and the opposing boundary. Subgroup analysis and multiple linear regression were used to evaluate the relationship between percentage changes of the LIVF dimensions and side, sex, and age.

Results: The LIVF dimensions varied significantly between the two positions at all levels (P < 0.05). From neutral to hyperextension supine position, the foraminal area reduced by 20.1% at lumbar 1-2 (L ), 22.6% at L , 19.9% at L , 18.1% at L , and 12.0% at lumbar 5-sacral 1 (L -S ) level, respectively; the foraminal height reduced by 9.5% at L , 10.5% at L , 9.5% at L , 9.6% at L , and 6.1% at L -S level, respectively; the foraminal width reduced by 12.8% at L , 14.5% at L , 13.0% at L , 10.4% at L , and 8.4% at L -S level, respectively. The changes in LIVF dimensions were biggest at L level and smallest at L -S level. Subgroup analysis showed that there were no significant differences in the percentage changes of LIVF dimensions between the sexes and sides (P > 0.05). Multiple linear analysis showed that the percentage changes of LIVF dimensions were not related to side, sex, and age (P > 0.05).

Conclusion: The dimensions of the LIVF showed significant decrease at all levels in the DAA-specific hyperextension supine position compared with the neutral supine position, and the percentage changes of LIVF dimensions were not influenced by side, sex, and age.
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http://dx.doi.org/10.1111/os.12728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7454148PMC
August 2020

Three-point Method to Guide the Tibial Resection and Component Placing in Total Knee Arthroplasty.

Orthop Surg 2020 Jun;12(3):861-868

Department of Orthopaedic Surgery, The First Hospital of Jilin University, Changchun, China.

Objective: To introduce a three-point method combining the midpoint of the posterior cruciate ligament (PCL), the midsulcus of the tibial spines, and the midpoint of the anterior cruciate ligament (ACL) to determine appropriate tibial resection and component placing during TKA and to compare this method with Insall's 1/3 method.

Methods: A consecutive series of 128 knees that underwent TKA from January 2015 to August 2018 were analyzed. In one group (64 knees), the medial 1/3 of tibial tubercle (the Insall's traditional method) was used for tibial component alignment. In the other group (64 knees), the three-point line connecting the midpoint of the PCL, the midpoint of the tibial spines, and the midpoint of the ACL was used for tibial component alignment. Both groups used the anterior tibial tendon as the distal reference for tibial resection. The coronal alignment error of the tibial component was determined by the angle between the line parallel to the tibial component platform and the tibial mechanical axis measured on postoperative radiograph. The axial rotation error of the femoral or tibial component was the intersection angle between the transepicondylar axis (TEA) and a line tangent to the posterior edge of the femoral or tibial component measured on CT. The coronal and axial alignment errors were compared between the two groups.

Results: The average coronal alignment error of the tibial component in the three-point method group was 0.2° ± 1.4° versus - 0.9° ± 1.8°in the Insall's 1/3 method group (P < 0.001), and the mean absolute value in the three-point method group reduced by 37.3% compared to Insall's traditional method group. The average axial rotation error of the femoral component was 0.2° ± 1.2° in the three-point method group versus - 1.1° ± 1.7° in the Insall's 1/3 method group (P < 0.001), and the mean absolute value in three-point method group decreased by 43.9% compared to Insall's traditional method group. The average axial rotation error of the tibial component was 0.4° ± 1.4° versus - 1.4° ± 1.8° in the Insall's 1/3 method group (P < 0.001), and the mean absolute value in the three-point method group reduced by 35.5% compared to the Insall's traditional method group. The rates of rotation outliers were significantly lower in the three-point method group (P < 0.05).

Conclusion: The line connecting the midpoint of the PCL, the midsulcus of the tibial spines, and the midpoint of the ACL could be used as the reference for the tibial resection and component placing. This method appears to be more accurate than Insall's 1/3 method. The results of this study provide a candidate method for component orientation with little error.
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http://dx.doi.org/10.1111/os.12693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307225PMC
June 2020

Factors influencing in-hospital death for pediatric patients with isolated methylmalonic acidemia: a nationwide inpatient database analysis.

Orphanet J Rare Dis 2020 06 19;15(1):154. Epub 2020 Jun 19.

National Clinical Research Centre for Digestive Diseases, Beijing Friendship Hospital, Capital Medical University, 95# Yong-an Road, Xi Cheng District, Beijing, 100050, China.

Background: Patients with isolated methylmalonic acidemia (MMA) usually experience recurrent episodes of acute metabolic decompensation or metabolic stroke, require frequent hospitalization, and have a relatively high mortality rate. The aim of our study was to assess factors predicting the in-hospital death of pediatric patients with isolated MMA. We performed a retrospective study using data from the Hospital Quality Monitoring System, a national inpatient database in China collected from 2013 to 2017. All patients under 18 years old with a diagnosis of isolated MMA were included. Demographic, hospital-related, and clinical features were collected. Poisson regression was performed to identify potential influencing variables associated with in-hospital death.

Results: From 2013 to 2017, among 2317 admissions for pediatric patients diagnosed with isolated MMA, 1.77% had the outcome of death. In the univariate analysis, patients aged under 1 year had a higher risk of death than did those aged 1 year or older (odds ratio [OR] = 2.63, 95% confidence interval [CI]: 1.36-5.07). There was a higher risk of in-hospital death for patients admitted through emergency departments or via referrals than for those admitted through other routes (OR = 3.76, 95% CI: 1.84-7.67). Deaths were higher in hospitals with volumes of less than 50 patients with isolated MMA during the five study years (OR = 2.92, 95% CI: 1.46-5.83). Moreover, the risk of in-hospital death gradually decreased over time (OR = 0.72, 95% CI: 0.57-0.90). In the multivariate analysis, the abovementioned associations with the risk of in-hospital death remained statistically significant. However, no significant associations were observed between specific clinical signs and in-hospital death in either the univariate or the multivariate analysis.

Conclusions: Younger age, admission to hospitals with low patient volumes, and admission through emergency departments or referrals are associated with higher risk of in-hospital death. The co-existence of specific clinical signs appears to have no effect on in-hospital death.
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http://dx.doi.org/10.1186/s13023-020-01446-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304167PMC
June 2020

Association of high-resolution computed tomography score with ventilator weaning and 28-day mortality of patients with acute respiratory distress syndrome.

J Int Med Res 2020 Jun;48(6):300060520912966

Neurosurgical ICU, Xianyang Hospital of Yan'an University, Xianyang, Shaanxi Province, China.

Objective: This study was performed to explore the association of the high-resolution computed tomography (HRCT) score with ventilator weaning and 28-day mortality of patients with acute respiratory distress syndrome (ARDS).

Method: In total, 197 patients treated for ARDS from October 2004 to December 2015 were retrospectively analyzed. Univariate analysis and multifactor regression analysis were used to determine the relationship of the HRCT score with ventilator weaning and 28-day mortality. Curve-fitting analysis and threshold analysis were further used to explore the association of the HRCT score with ventilator weaning and 28-day mortality.

Results: The multifactor regression analysis showed that the HRCT score was significantly associated with a lower rate of ventilator weaning and a higher risk of 28-day mortality in patients with ARDS. HRCT scores of 257.0 and 243.2 were the thresholds for ventilator weaning and 28-day mortality, respectively. When the HRCT score was below the threshold, every 1-point increase in the HRCT score was associated with a 4.6% decrease in the ventilator weaning rate and a 4.6% increase in the 28-day mortality rate.

Conclusion: The HRCT score was associated with ventilator weaning and 28-day mortality with a threshold of 257.0 and 243.2 points, respectively.
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http://dx.doi.org/10.1177/0300060520912966DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288822PMC
June 2020

A turn-on fluorescence strategy for biothiols determination by blocking Hg(II)-mediated fluorescence quenching of adenine-rich DNA-templated gold nanoclusters.

Luminescence 2020 Dec 17;35(8):1296-1303. Epub 2020 Jun 17.

College of Chemistry and Chemical Engineering, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China.

Fluorescent adenine (A)-rich DNA-templated gold nanoclusters were demonstrated to be a novel probe for determination of biothiols (including cysteine, glutathione, and homocysteine). Fluorescence intensity of adenine-rich DNA-templated gold nanoclusters could be greatly quenched by Hg(II) ions through the formation of a gold nanoclusters-Hg(II) system. When biothiols (cysteine as the model) were introduced into the system, the fluorescence intensity recovered due to the formation of a more stable Hg(II)-thiol coordination complex using Hg-S metal-ligand bonds, which inhibited the Hg(II)-mediated fluorescence quenching of adenine-rich DNA-templated gold nanoclusters. Based on this fluorescence phenomenon, an on-off-on fluorescence strategy was designed for the sensitive determination of biothiols. The method allowed sensitive detection of cysteine with a linear detection range from 100 nM to 5 μM and a limit of detection of 30 nM. Additionally, the assay can be applied for detection of biothiol levels in human plasma samples. Therefore, it can provide a simple and rapid fluorescent platform for biothiol detection.
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http://dx.doi.org/10.1002/bio.3891DOI Listing
December 2020

EGFR Gene Mutation and Methodological Evaluation in 399 Patients with Non-small Cell Lung Cancer.

Curr Med Sci 2020 Feb 13;40(1):78-84. Epub 2020 Mar 13.

Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

The purpose of the present study was to study the characteristics of epidemic growth factor receptor (EGFR) gene distribution in patients with non-small cell lung cancer (NSCLC), and to detect the mutation rate of EGFR gene by Sanger sequencing and amplification refractory mutation system (ARMS)-PCR. Paraffin-embedded sections of NSCLC tissues from 399 NSCLC patients diagnosed in Renmin Hospital of Wuhan University were collected, 103 of them were detected for exons 18-21 mutation of EGFR by Sanger sequencing method, 296 cases were detected for exons 18-21 mutation by ARMS-PCR method. DNA extraction of both groups was performed with Qiagen QLAamp DNA FFPE Tissue KIT. Comparisons of detection rates between the two methods were conducted by row X list chi-square test. The total mutation rate of EGFR gene detected by Sanger sequencing was 21.4%, exons 18-21 and combined mutation rates were 1.0%, 9.7%, 1.0%, 7.8% and 2.0%, respectively. And the proportions were 4.7%, 45.2%, 4.7%, 36.3% and 9.4% respectively. The total mutation rate detected by ARMS-PCR was 51.4%, exons 18-21 and combined mutation rates were 2.7%, 27%, 1.7%, 18.2% and 1.7%, respectively. The proportions were 5.3%, 52.6%, 3.3%, 35.5% and 3.3% respectively. Further analysis of mutation rate showed that there was significant difference between the two methods in detecting total mutation of EGFR gene (P<0.001). There were significant differences in mutation detection rates of exons 19 and 21 (P<0.001, P<0.05), but there were no significant differences in other exons. And there was no significant difference in mutation detection rates between the two methods. The mutation rate of EGFR gene in NSCLC patients was 50%. And exon 19 deletion was the most common mutation type, followed by exon 21 mutation. Compared with Sanger sequencing method, ARMS method is more sensitive with significant advantages in detecting exon 19 deletions and exon 21 mutations, which can be widely used in clinical detection of EGFR gene mutations. The results of this study will further guide patients with advanced NSCLC to select TKI targeted drugs, and provide clinical diagnostic basis for targeted therapy of NSCLC patients.
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http://dx.doi.org/10.1007/s11596-020-2149-5DOI Listing
February 2020

One-step synthesis of zerovalent-iron-biochar composites to activate persulfate for phenol degradation.

Water Sci Technol 2019 Nov;80(10):1851-1860

Liaoning Engineering Research Center for Treatment and Recycling of Industrially Discharged Heavy Metals, Shenyang University of Chemical Technology, Shenyang 110142, Liaoning, China E-mail:

A novel zerovalen-iron-biochar composite (nZVI/SBC) was synthesized by using FeCl-laden sorghum straw biomass as the raw material via a facile one-step pyrolysis method without additional chemical reactions (e.g., by NaBH reduction or thermochemical reduction). The nZVI/SBC was successfully employed as an activator in phenol degradation by activated persulfate. XRD, SEM, N adsorption-desorption and atomic absorption spectrophotometry analysis showed that the nanosized Fe was the main component of the 4ZVI/SBC activator, which was a mesopore material with an optimal FeCl·6HO/biomass impregnation mass ratio of 2.7 g/g. The 4ZVI/SBC activator showed an efficient degradation of phenol (95.65% for 30 min at 25 °C) with a large specific surface area of 78.669 m·g. The recovery of 4ZVI/SBC activator after the degradation reaction of phenol can be realized with the small amount of dissolved iron in the water. The 4ZVI/SBC activator facilitated the activation of persulfate to degrade phenol into non-toxic CO and HO. The trend of Cl, SO and NO affected the removal efficiency of phenol by using the 4ZVI/SBC activator in the following order: NO > SO > Cl. The one-step synthesis of the nanosized zerovalent-iron-biochar composite was feasible and may be applied as an effective strategy for controlling organic waste (e.g. phenol) by waste biomass.
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http://dx.doi.org/10.2166/wst.2020.001DOI Listing
November 2019

Highly selective fluorimetric and colorimetric sensing of mercury(II) by exploiting the self-assembly-induced emission of 4-chlorothiophenol capped copper nanoclusters.

Mikrochim Acta 2020 02 22;187(3):185. Epub 2020 Feb 22.

College of Chemistry and Chemical Engineering, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People's Republic of China.

A highly stable copper nanoclusters (CuNC) carrying 4-chlorothiophenol as a protective ligand is described. They display self-assembly-induced emission with excitation/emission maxima at 330/605 nm even in neutral or alkaline aqueous environment. The fluorescence of these CuNC is quenched by Hg(II). Quenching is mainly ascribed to the formation of a complex formed via Hg-S bonding between the Hg(II) ions and the ligand. This destroys the ordered architectures of the assembled CuNC. The assay enables Hg(II) to be determined with good sensitivity and a linear response ranging from 1 to 500 nM Hg(II) with a 0.3 nM limit of detection. In addition, the method was implemented in a test strip (which undergoes a color change from red to blue) that can be used for visual determination of Hg(II) in complex environmental water samples. Graphical abstractNovel and highly selective fluorimetric and colorimetric methods have been designed for mercury(II) ions determination based on stable self-assembly-induced emission of copper nanoclusters.
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http://dx.doi.org/10.1007/s00604-020-4158-2DOI Listing
February 2020

MR imaging as a precise technique to evaluate skull-base tumor volume: Comparison of CT, MR imaging and FDG PET from murine and clinical data.

J Craniomaxillofac Surg 2020 Jan 13;48(1):105-110. Epub 2019 Dec 13.

Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China; National Clinical Research Center for Oral Diseases, Beijing, 100081, PR China; National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, 100081, PR China; Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, 100081, PR China. Electronic address:

In spite of the many imaging modalities used in clinics, the one that best reflects the true delineation of skull-base (infratemporal fossa, ITF) malignancies is still unknown. In order to compare the tumor recognition capabilities of different imaging modalities, established murine models and patients with skull-base tumors were evaluated by computer tomography (CT), magnetic resonance (MR) imaging, and fluorine-18 fluorodeoxyglucose (FDG) positron emission tomography (PET) for delineation of gross tumor volume (GTV). PET, MR imaging, and CT enhanced by iodine staining were all sensitive to, and able to recognize, the skull-base tumor in the murine model. No significant difference (p > 0.9999) was observed between average GTV according to MR imaging (176.67 ± 19.6 mm) and the histological measurement result (170.23 ± 22.24 mm) for the murine model. In contrast, the GTVs according to CT (88.77 ± 13.03 mm, p < 0.0001) and FDG PET (35.67 ± 6.56 mm, p < 0.0001) were much smaller. In nine patients for whom the three modalities were available, tumor volume comparisons tended to be consistent with the murine model data. According to both the established murine model and clinical patient data, MR imaging possessed the optimal ability to recognize tumor contours.
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http://dx.doi.org/10.1016/j.jcms.2019.12.007DOI Listing
January 2020

Correction to: Arsenic concentration, speciation, and risk assessment in sediments of the Xijiang River basin, China.

Environ Monit Assess 2019 Dec 19;192(1):56. Epub 2019 Dec 19.

Liaoning Engineering Research Center for Treatment and Recycling of Industrially Discharged Heavy Metals, Shenyang University of Chemical Technology, Shenyang, 110142, People's Republic of China.

In the original paper, there was an error in the communication unit 1. The communication unit was "Liaoning Engineering Research Center for Treatment and Recycling of Industrially Discharged Heavy Metals, Shenyang University of Chemical Technology, Shenyang 110142, People's Republic of China".
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http://dx.doi.org/10.1007/s10661-019-7965-3DOI Listing
December 2019

Methylmalonic and propionic acidemia among hospitalized pediatric patients: a nationwide report.

Orphanet J Rare Dis 2019 12 16;14(1):292. Epub 2019 Dec 16.

National Clinical Research Centre for Digestive Diseases, Beijing Friendship Hospital, Capital Medical University, 95# Yong-an Road, Xi Cheng District, Beijing, 100050, China.

Background: Methylmalonic acidemia (MMA) and propionic acidemia (PA) are two kinds of diseases caused by inborn errors of metabolism. So far, the epidemiological data on them are limited in China. The aim of our study is to investigate the proportion and characteristics of hospitalized pediatric patients with MMA and PA in China.

Methods: The data in this study were obtained from the Hospital Quality Monitoring System, a national inpatient database in China, with information on the patients hospitalized during the period from 2013 to 2017. We identified the data related to the patients who were under 18 years old and were diagnosed with MMA/PA, and extracted the information on demographic characteristics, hospital location, total cost and other related clinical presentations from the data.

Results: Among all hospitalized pediatric patients with liver diseases, there were increasing trends in the proportion of individuals diagnosed with MMA or PA during the period from 2013 (0.76% for MMA; 0.13% for PA) to 2017 (1.61% for MMA; 0.32% for PA). For both MMA and PA, children under 2-year-old accounted for the highest proportion. The median of total cost per hospitalization was relatively high (RMB 7388.53 for MMA; RMB 4999.66 for PA). Moreover, most patients hospitalized in tertiary class A hospitals (MMA: 80.96%, PA: 76.21%); and a majority of pediatric patients admitted in the hospitals in Shanghai and Beijing are from outside districts. Manifestations of nervous system-related symptoms, and metabolic acidosis or anemia in laboratory findings were more common during hospitalization.

Conclusions: The study is the first nationwide one in providing epidemiological and clinical information on hospitalized pediatric patients with MMA/PA. An increasing hospitalization with various presentations and a heavy financial burden were observed. In addition, geographically, the medical resources in China have been unevenly distributed.
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http://dx.doi.org/10.1186/s13023-019-1268-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915987PMC
December 2019

The establishment and clinical evaluation of a novel, rapid, no-wash one-step immunoassay for the detection of dengue virus non-structural protein 1.

J Virol Methods 2020 02 30;276:113793. Epub 2019 Nov 30.

Key Laboratory of Emerging Infectious Disease Detection, Zhuhai International Travel Healthcare Center, Gongbei Customs District, Zhuhai 519020, Guangdong, China. Electronic address:

Dengue fever is a highly endemic arthropod-borne viral disease in the tropical and sub-tropical countries and is rapidly becoming a global threaten. Diagnosis has been conducted by either traditional serological methods or molecular biological techniques. However, these methods are either labor-intensive, time-consuming or with multiple steps, which are not suitable for high throughput detection of large quantity of samples. In the current study, a novel, rapid, no-wash one-step amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) was developed and optimized for the diagnosis of dengue fever through the detection of dengue virus non-structural protein 1 (NS1). The linear range of the assay was determined to be 60,000 pg/mL to 200 pg/mL, with a lower detection limit of 127.45 pg/mL for NS1 protein. The precision of the assay was 8.24 % and 4.93 % for the high and low concentration. Clinical evaluation indicated that the sensitivity and specificity of the assay was 91.49 % and 81.54 %, respectively. This novel, rapid, no-wash one-step AlphaLISA assay is convenient and sensitive, which could be a good alternative for the screening of dengue fever in a high throughput format.
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http://dx.doi.org/10.1016/j.jviromet.2019.113793DOI Listing
February 2020

An unusual case of cauliflower-like tumor of breast.

Chin Med J (Engl) 2019 Nov;132(22):2767-2769

Department of Thoracic Cardiovascular Surgery, Central Theater Command General Hospital of the Chinese People's Liberation Army, Wuhan, Hubei 430070, China.

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http://dx.doi.org/10.1097/CM9.0000000000000516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940108PMC
November 2019

Characterizing the Role of in Salt Tolerance.

Int J Mol Sci 2019 Nov 14;20(22). Epub 2019 Nov 14.

Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China.

The WRKY transcription factor superfamily is known to participate in plant growth and stress response. However, the role of this family in wheat ( L.) is largely unknown. Here, a salt-induced gene was identified in an RNA-Seq data set from salt-treated wheat. The results of RT-qPCR analysis showed that was significantly induced in NaCl-treated wheat and reached an expression level of about 22-fold of the untreated wheat. Then, a further functional identification was performed in both and L. Subcellular localization analysis indicated that TaWRKY13 is a nuclear-localized protein. Moreover, various stress-related regulatory elements were predicted in the promoter. Expression pattern analysis revealed that can also be induced by polyethylene glycol (PEG), exogenous abscisic acid (ABA), and cold stress. After NaCl treatment, overexpressed lines of have a longer root and a larger root surface area than the control (Columbia-0). Furthermore, overexpression rice lines exhibited salt tolerance compared with the control, as evidenced by increased proline (Pro) and decreased malondialdehyde (MDA) contents under salt treatment. The roots of overexpression lines were also more developed. These results demonstrate that plays a positive role in salt stress.
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http://dx.doi.org/10.3390/ijms20225712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888956PMC
November 2019
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