Publications by authors named "Hai-Yan Wang"

468 Publications

Determination of Fe(Ⅲ) ion and cellular bioimaging based on a novel photoluminescent silicon nanoparticles.

Talanta 2021 Aug 21;230:122294. Epub 2021 Mar 21.

College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China; National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

The determination approaches of Fe (Ⅲ) in biological samples were developed by a novel water-soluble silicon nanoparticles (SiNPs). The SiNPs were synthesized by a facile microwave-assisted method, and simultaneously featured strong blue fluorescence (photoluminescence quantum yield: 25.2%), long lifetime (~13.29 ns) and good photo-stability. The fluorescence intensities of SiNPs were gradually quenched with Fe (Ⅲ) concentration increasing from 2.0 to 50 μmol/L. The detection limit of the established method was 0.56 μmol/L and the precision for eleven replicate detections of 20 μmol/L Fe (Ⅲ) was 3.2% (relative standard deviation, RSD). The spiked recoveries were 99.0%-104.5%. Results of the lifetime decay and cyclic voltammetry (CV) evidenced that the electron transfer was responsible for the fluorescence quenching mechanism of SiNPs and Fe (Ⅲ). Moreover, the SiNPs were successfully applied in the determination of Fe(Ⅲ) in different environmental waters and human serum. Finally, the resulting SiNPs exhibited the green fluorescence in HeLa cells as the optical probe.
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http://dx.doi.org/10.1016/j.talanta.2021.122294DOI Listing
August 2021

Mitochondria targeted near-infrared chemodosimeter for upconversion luminescence bioimaging of hypoxia.

Chem Commun (Camb) 2021 Apr 28. Epub 2021 Apr 28.

School of Engineering, China Pharmaceutical University, Nanjing, 211198, China.

We report a mitochondria-targeted near-infrared probe (NRh-O) for frequency upconversion luminescence (FUCL) imaging of hypoxia. Under hypoxic conditions, NRh-O rapidly responds to release the FUCL product NRh (λex/em = 850/825 nm) with high sensitivity and selectivity in mitochondria. This highlights the potential application of a hypoxia-responsive probe in early clinical diagnosis.
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http://dx.doi.org/10.1039/d1cc01338fDOI Listing
April 2021

[Release Mechanisms of Carbon Source and Dissolved Organic Matter of Six Agricultural Wastes in the Initial Stage].

Huan Jing Ke Xue 2021 May;42(5):2422-2431

Research Center of Environmental Pollution Control Technology, Chinese Research Academy of Environmental Science, Beijing 100012, China.

As carbon sources for the denitrification process, agricultural wastes have some problems, such as excess release of organic carbon; unclear release characteristics of nitrogen, phosphorus, and colorimetric substances; and unclear components, release mechanisms, and potential effects of the released dissolved organic matter (DOM) in the start-up period. To resolve those problems, rice straw, wheat straw, corn stalk, corncob, soybean stalk, and soybean hull were selected as denitrification carbon sources to investigate the release mechanisms and potential influences of the organic matter, secondary pollutants, and DOM. The results showed that the six agricultural wastes could be used as the denitrification carbon source. The carbon content in the wheat straw was the highest and the secondary pollution risk from the corncob was the lowest. For the six carbon sources, the second-order kinetic equation and Ritger-Peppas equation were followed during the 1-120 h carbon release process. The fitting results demonstrated that corncob was more suitable for use as the denitrification carbon source because of its moderate value and longer value, and the release mechanisms of the six types of carbon sources were mainly controlled by the diffusion process. The NH-N, TN, and TP contents in the immersion water of the rice straw were higher than those of the five other agricultural wastes, and there was heavy chromaticity in the immersion water of the wheat straw and corn stalk. The amounts of nitrogen, phosphorus, and chromatic substances released from the corncob were the lowest. The leachates of the corncob and soybean hull had higher biodegradability and lower risks of secondary pollution than those of the other sources. The aromaticity and molecular weight of DOM increased as the reaction time increased, and the humification of DOM was low. Five components were identified by PARAFAC. The main component was protein-like matter, which was mainly composed of tyrosine-like and tryptophan-like substances. There was less humic acid-like matter in the immersion water. The component characteristics of DOM might have had an adverse effect on the subsequent water treatment process. These results could provide theoretical support for the impact on effluent water quality and risk assessment when the agricultural wastes are used as an additional denitrification carbon source at the start-up stage.
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http://dx.doi.org/10.13227/j.hjkx.202010177DOI Listing
May 2021

Baseline characteristics of myopic choroidal neovascularization in patients above 50 years old and prognostic factors after intravitreal conbercept treatment.

Sci Rep 2021 Apr 1;11(1):7337. Epub 2021 Apr 1.

Department of Ophthalmology, Xijing Hospital, Xi'an, 710032, Shaanxi, China.

To investigate the influence of age on the function and morphology of patients with myopic choroidal neovascularization (mCNV) and to evaluate the effect and prognostic factors of recurrence of Conbercept treatment on mCNV patients over 50 years. A total of 64 patients (64 eyes) with mCNV were enrolled in this retrospective study. The differences in baseline best-corrected visual acuity (BCVA) and morphological features on imaging between the younger group (˂ 50 years) and the older group (≥ 50 years) were analyzed. Of all, 21 eyes of 21 mCNV patients aged over 50 years who received Conbercept injection were further analyzed. Between the younger and the older group, significant differences were shown in mean BCVA (0.58 ± 0.28 vs 0.77 ± 0.31), subfoveal choroidal thickness (SFCT) (108.17 ± 78.32 μm vs 54.68 ± 39.03 μm) and frequency of vitreoretinal interface abnormalities (VIA) (2 vs 13), respectively (P < 0.05). After treated with Conbercept, the mean BCVA of 21 older mCNV patients increased from 0.83 ± 0.30 at baseline to 0.49 ± 0.24 at one year. Baseline BCVA, external limiting membrane damage, CNV area and CNV location correlated with the visual acuity at the 1-year follow-up. There were 7 (33.3%) recurrent cases during the follow-up and the risk of recurrence in patients with baseline central macular thickness (CMT) ≥ 262.86 μm was 14 times greater than that of patients with CMT < 262.86 μm. The risk of recurrence increased 1.84 times for every 100-μm increment in the CMT. Patients over 50 years with mCNV had a worse BCVA, thinner choroid, and higher risk of VIA than young mCNV patients. The standard Conbercept treatment strategy was safe and effective in mCNV patients over 50 years. As patients over 50 years with a greater CMT have a high risk of recurrence, more attention should be paid on these patients by following them up closely.
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http://dx.doi.org/10.1038/s41598-021-86835-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016885PMC
April 2021

[Clinical effect and safety of moxibustion therapy in treatment of mild cognitive impairment: a multi-center randomized controlled trial].

Zhen Ci Yan Jiu 2020 Oct;45(10):851-5

School of Acupuncture and Massage, Chengdu University of TCM, Chengdu 610036, China; Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of Chengdu University of TMC, Chengdu 610075.

Objective: To investigate the clinical effect and safety of moxibustion therapy based on Sancai theory for reinforcing intelligence in the early intervention of mild cognitive impairment (MCI).

Methods: A total of 210 patients with MCI were divided into moxibustion group and medication group using a random number table, with 105 patients in each group. The patients in the moxibustion group were given moxibustion at Baihui (GV20), Shenque (CV8), and Yongquan (KI1) once every other day, 20 minutes each time, for a total of 8 weeks, and those in the medication group were given oral Nimodipine 30 mg once a day for 8 weeks. Mini-Mental State Examination (MMSE) score, score of meaningless graphics recognition of Clinical Memory Scale (CMS), and event-related potential P300 latency were evaluated before treatment, after 8 weeks of treatment, and at 12 weeks after treatment, and the safety of treatment was analyzed for both groups.

Results: Both groups had significant increases in MMSE score and the score of meaningless graphics recognition of CMS and a significant reduction in P300 latency after treatment and during follow-up (<0.01,<0.05). Compared with the medication group, the moxibustion group had a significant increase in MMSE score and a significant reduction in P300 latency after treatment (<0.05).

Conclusion: Moxibustion therapy based on Sancai theory for reinforcing intelligence can improve cognitive impairment in patients with MCI and is thus an effective intervention method for improving the cognitive function of patients with MCI.
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http://dx.doi.org/10.13702/j.1000-0607.191012DOI Listing
October 2020

Dissolution and processing of silk fibroin for materials science.

Crit Rev Biotechnol 2021 May 21;41(3):406-424. Epub 2021 Mar 21.

Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China.

In recent decades, silk fibroin (SF) from silkworm has been extensively researched and applied in several fields, including: cosmetics, biomedicine and biomaterials. The dissolution and regeneration of SF fibers is the key and prerequisite step for the application of silk protein-based materials. Various solvents and dissolving systems have been reported to dissolve SF fibers. However, the dissolution process directly affects the characteristics of SF and particularly impacts the mechanical properties of the resulting silk biomaterials in subsequent processing. The purpose of this review is to summarize the common solvents, the dissolution methods for silk protein, the properties of the resulting SF protein. The suitable use of SF dissolved in the corresponding solvent was also briefly introduced. Recent applications of SF in various biomaterials are also discussed.
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http://dx.doi.org/10.1080/07388551.2020.1853030DOI Listing
May 2021

A computational and experimental investigation of donor-acceptor BODIPY based near-infrared fluorophore for in vivo imaging.

Bioorg Chem 2021 May 4;110:104789. Epub 2021 Mar 4.

College of Chemistry and Chemical Engineering, Key Laboratory of Functional Inorganic Materials of Anhui Province, Anhui University, Hefei 230039, PR China. Electronic address:

TD-DFT quantum calculation was performed to predict and/or illustrate the electronic transition, the related absorption and emission maxima of some pyrrole-difluoroboron derivatives with different electron donor-acceptor unit or π-conjugated degree. Upon the calculated results, a new near infrared (NIR) fluorophore (abbreviated as TPBD-BP) was designed and fabricated through linking triphenylamine and pyrrole-difluoroboron units to benzothiadiazole (BTD) backbone. The fluorescence of TPBD-BP in solid state centered at 932 nm, which was 985 nm for TPBD-BP nanoparticles (TPBD-BP dots) encapsulated in PEG-6000. The fluorescence of TPBD-BP in both solid state and dots exhibited off-peak tail emission to NIR-II region (extended to 1300 nm). The TPBD-BP dots showed excellent water solubility, biocompatibility and aggregation induced emission (AIE), which was suitable to be applied in vivo imaging. NIR-II emission signal of TPBD-BP dots can be observed in the reproductive organ of normal nude mice after tail vein injection. This attractive combination of computational and experimental investigation would help to develop new-typed small-molecular NIR fluorophores.
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http://dx.doi.org/10.1016/j.bioorg.2021.104789DOI Listing
May 2021

Characterization of undegraded and degraded silk fibroin and its significant impact on the properties of the resulting silk biomaterials.

Int J Biol Macromol 2021 Apr 16;176:578-588. Epub 2021 Feb 16.

Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, PR China. Electronic address:

The silk fibroin (SF) regeneration process significantly affects the resulting biomaterials, unfortunately, there has been insufficient study regarding the most suitable regeneration method for SF. In this study, we prepared undegraded SF (uSF) and degraded SF (dSF) by common regeneration methods and studied their difference in detail. The results demonstrated that the degradation degree of SF peptide chain had little influence on the secondary structure and thermal stability of SF materials. While, uSF solution showed higher viscosity and surface tension than dSF solution. The uSF membrane (uSFM) could be elongated approximately 134%, 1.6 times the degraded SF membrane (dSFM). SEM implied that both uSF and dSF existed in aqueous solution as micelles with a diameter of approximately 30 nm. dSF could directly form SF nanoparticles (dSFNPs) when poured into acetone while uSF could only form nanoparticles (uSFNP) with the addition of SDS. Glucose oxidase embedded into dSFM and dSFNP showed high catalytic activities, but uSFNP demonstrated nearly no activity. In addition, the dSFM was more appropriate for L929 cell culture. Considering the obvious difference between the two SF proteins, our results are significant in guiding the application of appropriate SF proteins in tissue engineering materials, bioactive materials, bioink, etc.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.02.100DOI Listing
April 2021

Curcumin Regulated the Homeostasis of Memory T Cell and Ameliorated Dextran Sulfate Sodium-Induced Experimental Colitis.

Front Pharmacol 2020 1;11:630244. Epub 2021 Feb 1.

Formula-Pattern Research Center of Jiangxi University of Traditional Chinese Medicine, Nanchang, China.

Immune memory is protective against reinvasion by pathogens in the homeostatic state, while immune memory disorders can cause autoimmune disease, including inflammatory bowel disease. Curcumin is a natural compound shown to be effective against human inflammatory bowel disease and experimental colitis, but the underlying mechanism is unclear. Here, experimental colitis was induced by dextran sulfate sodium (DSS) in this study. Significant changes in the percentages of naïve, central memory T (TCM), and effector memory (TEM) cells and their CD4 and CD8 subsets were found in the peripheral blood of mice with colitis using flow cytometry. After 7 days of continuous curcumin (100 mg/kg/day) administration, the DSS-induced experimental colitis was effectively relieved, with significant decreases in the ratio of day weight to initial body weight, colonic weight, pathological injury score, levels of proinflammatory cytokines IL-7, IL-15, and IL-21, colonic mucosal ulceration, and amount of inflammatory infiltrate. Importantly, curcumin significantly restored the percentages of naïve, TCM, and TEM cells and their CD4 and CD8 subpopulations. In addition, curcumin significantly inhibited the activation of the JAK1/STAT5 signaling pathway, downregulation of JAK1, STAT5, and p-STAT5 proteins in colon tissue, and upregulation of PIAS1 proteins. These results suggested that curcumin effectively regulated the differentiation of naïve, TCM, and TEM cells in the peripheral blood to alleviate DSS-induced experimental colitis, which might be related to the inhibition of JAK1/STAT5 signaling activity.
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http://dx.doi.org/10.3389/fphar.2020.630244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882737PMC
February 2021

Homochiral fluorescence responsive molecularly imprinted polymer: Highly chiral enantiomer resolution and quantitative detection of L-penicillamine.

J Hazard Mater 2021 Jun 28;412:125249. Epub 2021 Jan 28.

College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China; National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

In this work, we innovatively synthesized homochiral fluorescence nano molecularly imprinted polymers (D-MIP) with dual affinity (metal ion affinity and homochiral affinity) for the specific separation and detection of L-penicillamine (L-PA), which is a core-shell structure with a SiO-covered CDs core and an imprinted layer with L-PA cavities. A switch for fluorescence response was built by chelating grafted Cu, what's more, the imprinted L-PA was pre immobilized by Cu to form the directional imprinting with predetermined spatial structure. More importantly, the homochiral affinity of D-galactose in homochiral molecularly imprinted polymers (D-MIP) greatly enhanced the selective adsorption of L-PA, and D-MIP showed a high selectivity factor (α) of 3.45, which is 1.9 times that of the non-homochiral molecularly imprinted polymers (MIP). Meanwhile, D-MIP exhibited a high enantiomeric excess (ee) value of 56% for separation of racemic PA. Additionally, a high sensitive and selective method was established for the detection of L-PA.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125249DOI Listing
June 2021

Kynurenine promotes the cytotoxicity of NK cells through aryl hydrocarbon receptor in early pregnancy.

J Reprod Immunol 2021 02 2;143:103270. Epub 2021 Jan 2.

Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, 200011, People's Republic of China; Key Laboratory of Reproduction Regulation of NPFPC, SIPPR, IRD, Fudan University, Shanghai, 200032, People's Republic of China; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, People's Republic of China. Electronic address:

During early pregnancy, decidual NK (dNK) cells play indispensable roles in many processes including the decidualization, the implantation, and the maintenance of immune tolerance. Abnormal cytotoxic activity of NK cells can cause recurrent spontaneous abortion (RSA), while the regulatory mechanism of NK cytotoxicity remains to be unclear. In this study, we found that kynurenine in decidua and villus was in a comparable level between patients with RSA and normal pregnancy women. However, the aryl hydrocarbon receptor (AhR) in decidual NK cells was significantly increased in RSA. Compared with AhR NK cells, cytotoxic activity-related molecules (NKP30, NKP46, NKG2D, perforin, granzyme B and IFN-γ) was highly expressed in both AhR peripheral and decidual NK cells, and kynurenine stimulation promoted the expression of killer receptors and the cytoplasmic granules in an AhR-dependent manner. Stimulation with TNF-α, IL-β and LPS upregulated the AhR expression in dNK cells in vitro. These results indicate that kyn/AhR signal enhances the cytotoxicity of NK cells, and increased expression of AhR may be an induction factor of RSA.
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http://dx.doi.org/10.1016/j.jri.2020.103270DOI Listing
February 2021

Study on Clinical Significance of LncRNA EGOT Expression in Colon Cancer and Its Effect on Autophagy of Colon Cancer Cells.

Cancer Manag Res 2020 31;12:13501-13512. Epub 2020 Dec 31.

Department of General Surgery, North China University of Science and Technology Affiliated Hospital, Tangshan City, Hebei Province 063000, People's Republic of China.

Background: Colon cancer (CC) is a common digestive tract tumor, and the increase of new and dead patients every year still puzzles clinical workers. LncRNA eosinophil granule ontogeny transcript (), as a newly discovered long-chain noncoding RNA (lncRNA), is differentially expressed in other tumors, but there are fewer studies of it in colon cancer.

Methods: The relative expression and diagnostic value of in CC were detected and analyzed by starBase online website and qRT-PCR. The patients were followed-up for five years, and Cox regression was used to analyze the independent prognostic factors of CC. The effects of overexpression (pcDNA-RGOT) on CC cell function were detected by CCK-8, transwell and flow cytometry. WB was applied to detect autophagy. The influence of knocking out (sh-) on tumor growth was observed by tumor allogeneic inhibition. The microRNA (miR) and mRNA in the downstream of were predicted and the ceRNA network map was drawn.

Results: The online database and qRT-PCR detection showed that was highly expression in patients with CC and had good diagnostic value. The five-year survival rate of patients with high expression of decreased. and TNM staging were independent prognostic factors of patients with CC. Functional analysis revealed that the growth and invasion abilities of cells increased, and the apoptosis rate decreased after overexpression. Upregulation of inhibited autophagy of CC cells and promoted cell growth. However, the tumor in nude mice was significantly lessened after knockout of . Bioinformatic analysis showed that microRNA-33a-5p and microRNA-33b-5p had targeted binding sites with .

Conclusion: is highly expressed in CC and has high diagnostic value. In addition, inhibition of can promote autophagy of CC cells and inhibit cell growth and metastasis, which is expected to be a potential therapeutic index.
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http://dx.doi.org/10.2147/CMAR.S285254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781029PMC
December 2020

The IFN-γ-IDO1-kynureine pathway-induced autophagy in cervical cancer cell promotes phagocytosis of macrophage.

Int J Biol Sci 2021 1;17(1):339-352. Epub 2021 Jan 1.

Department of Gynecology of Integrated Traditional Chinese and Western Medicine, Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, 200011, People's Republic of China.

Cervical cancer is a common malignant disease in female patients accompanied by activation of autophagy in tumor cells. However, the exact regulatory factors of autophagy and its effects on the immune response remain unknown. The induction of autophagy in HeLa and SiHa cells treated with IFN-γ, tryptophan depletion, kynurenine and epacadostat was detected by western blot analysis and by an autophagy detection kit. Following co-culture with pre-treated HeLa and SiHa cells, U937 cells were analyzed by flow cytometry to detect CD80, CD86, CD163 and CD206 expression and the induction of phagocytosis. IFN-γ caused a significant increase in the autophagy levels of HeLa and SiHa cells by promoting indoleamine-2,3-dioxygenase-1 (IDO1) expression. The induction of phagocytosis in HeLa and SiHa cells and the expression levels of CD80 and CD86 in U937 cells were increased significantly following treatment with recombinant human IFN-γ. This effect was associated with the induction of tumor cell autophagy. IFN-γ treatment and IDO1 overexpression promoted tryptophan depletion and kynurenine accumulation in cervical cancer cells. The latter was more potent in inducing autophagy of cervical cancer cells and promoting phagocytosis of macrophages. , IDO1 overexpression restricted tumor growth in C57 mice and enhanced the induction of phagocytosis in macrophages. IFN-γ promoted induction of autophagy and macrophage phagocytosis in cervical cancer cells possibly via IDO1 expression and kynurenine metabolism.
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http://dx.doi.org/10.7150/ijbs.51241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757030PMC
January 2021

HA targeted-biodegradable nanocomposites responsive to endogenous and exogenous stimulation for multimodal imaging and chemo-/photothermal therapy.

Nanoscale 2021 Jan;13(2):886-900

College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China. and National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Multimodal imaging-guided accurate tumor-targeting and efficient synergistic therapy are of great importance for cancer therapy in vitro and in vivo. In this study, a biocompatible, tumor-targeted, on-demand chemo-/photothermal therapeutic nanoplatform (HIDSiGdNPs@PDA-HA) based on hollow mesoporous organic silica nanoparticles (HMONs) was used for bimodal imaging and multi-factor stepwise response for drug release and treatment. Targeted molecule hyaluronic acid (HA) promoted the endocytosis of HIDSiGdNPs@PDA-HA in HeLa cancer cells. The gatekeeper pH-/light-sensitive PDA coating was stimulated by the endogenous tumor acidic microenvironment and exogenous NIR laser to release doxorubicin (DOX). Thereafter, HMONs containing S-S bonds were reduced and degraded by endogenous glutathione (GSH), and the drug was further released rapidly to kill cancer cells. Importantly, the photothermal reagent indocyanine green (ICG) was always retained in the carrier, improving the effectiveness of photothermal therapy. The loaded Gd-doped silicon nanoparticles (SiGdNPs) combined with DOX and ICG led to multi-color fluorescence imaging in vitro and magnetic resonance imaging in vivo to realize targeted diagnosis and track drug distribution. The treatment results of tumor-bearing mice also proved the excellent synergistic therapy. It is believed that the multifunctional nanomaterials with dual mode imaging capability and targeted and controlled collaborative therapy would provide an alternative for accurate diagnosis and efficient treatment.
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http://dx.doi.org/10.1039/d0nr07121hDOI Listing
January 2021

[Effects of dazomet fumigation on growth, biological characteristics of seedlings and soil environment].

Ying Yong Sheng Tai Xue Bao 2020 Sep;31(9):3085-3092

College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China.

In this study, we examined the effects of dazomet fumigation with different concentrations (0, 0.1, 0.2, 0.4 g·kg) on the microbial characteristics of continuous cropping soil and growth of seedling in greenhouse and open-field pot. The results showed that all the treatment of dazomet fumigation could promote the growth of seedlings in continuous cropping soil, with 0.2 g·kg treatment showing the strongest effect. Compared to the control, plant height, stem diameter, dry weight of seedlings in 0.2 g·kg dazomet fumigation were increased by 192.9% and 91.8%, 72.8% and 60.1%, 196.8% and 195.0%, 138.5% and 130.7%, respectively in greenhouse and open-field. The root related indices (root length, root area, root volume, root respiration rate) were significantly promoted. The activities of SOD, POD, CAT in roots were increased by 114.6% and 118.5%, 123.5% and 107.6%, 164.6% and 175.6% respectively compared with the control, whereas the content of malondialdehyde was significantly lowered. Soil bacterial content, fungal content, copy number of gene and soil enzyme activity were significantly decreased with the increasing dazomet concentrations. In conclusion, 0.2 g·kg dazomet fumigation could increase the biomass of seedlings in continuous cropping, improve soil environment, and effectively alleviate the continuous cropping obstacle. Therefore, 0.2 g ·kg dazomet fumigation could be given priority during the reconstruction of old apple orchards.
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http://dx.doi.org/10.13287/j.1001-9332.202009.022DOI Listing
September 2020

An Integrated Electrochemical Nanodevice for Intracellular RNA Collection and Detection in Single Living Cell.

Angew Chem Int Ed Engl 2020 Dec 19. Epub 2020 Dec 19.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.

New tools for single-cell interrogation enable deeper understanding of cellular heterogeneity and associated cellular behaviors and functions. Information of RNA expression in single cell could contribute to our knowledge of the genetic regulatory circuits and molecular mechanism of disease development. Although significant progresses have been made for intracellular RNA analysis, existing methods have a trade-off between operational complexity and practical feasibility. We address this challenge by combining the ionic current rectification property of nanopipette reactor with duplex-specific nuclease-assisted hybridization chain reaction for signal amplification to realize a simple and practical intracellular nanosensor with minimal invasiveness, which enables single-cell collection and electrochemical detection of intracellular RNA with cell-context preservation. Systematic studies on differentiation of oncogenic miR-10b expression levels in non-malignant breast cells, metastatic breast cancer cells as well as non-metastatic breast cancer cells were then realized by this nanotool accompanied by assessment of different drugs effects. This work has unveiled the ability of electrochemistry to probe intracellular RNA and opened new opportunities to study the gene expression and heterogeneous complexity under physiological conditions down to single-cell level.
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http://dx.doi.org/10.1002/anie.202014798DOI Listing
December 2020

Preparation of glycan-oriented imprinted polymer coating Gd-doped silicon nanoparticles for targeting cancer Tn antigens and dual-modal cell imaging via boronate-affinity surface imprinting.

Talanta 2021 Feb 30;223(Pt 1):121706. Epub 2020 Sep 30.

College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin, 300071, China; National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. Electronic address:

Early and accurate detection of breast cancer plays an important role in improving the survival rates of patients. In this work, we designed and synthesized the Gal-NAc-imprinted nanoparticles (GIPs) via boronate-affinity glycan-oriented surface imprinting strategy. Molecularly imprinted polymers (MIPs) were hybridized with fluorescent silicon nanoparticles (SiNPs) to target Tn antigens. However, the single fluorescent imaging mode is not conducive to obtaining accurate diagnosis, due to its poor tissue penetration. To resolve this obstacle, doping gadolinium (Gd) into SiNPs was adopted to emerge an extra significant magnetic resonance (MR) signal, achieving highly sensitive fluorescence imaging and magnetic resonance imaging (MRI) with high spatial resolution. GIPs had uniform particle size around 31.8 nm, and exhibited satisfactory fluorescence stability. The maximum adsorption capacity of GIPs was 1.15 μM/g with a high imprinting factor (IF) of 7.5. Confocal laser scanning microscope imaging revealed that the GIPs had excellent specific recognition ability with a low cytotoxicity. GIPs also showed an outstanding MR performance on cancer cells. Therefore, the synthesized nanoparticles had desirable performance in dual-model imaging to specifically target recognition cancer cells. It may have a tremendous potential in real biological samples.
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http://dx.doi.org/10.1016/j.talanta.2020.121706DOI Listing
February 2021

Target-Triggered Assembly in a Nanopipette for Electrochemical Single-Cell Analysis.

Anal Chem 2021 01 10;93(2):1200-1208. Epub 2020 Dec 10.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Engineered nanopipette tools have recently emerged as a powerful approach for electrochemical nanosensing, which has major implications in both fundamental biological research and biomedical applications. Herein, we describe a generic method of target-triggered assembly of aptamers in a nanopipette for nanosensing, which is exemplified by sensitive and rapid electrochemical single-cell analysis of adenosine triphosphate (ATP), a ubiquitous energy source in life and important signaling molecules in many physiological processes. Specifically, a layer of thiolated aptamers is immobilized onto a Au-coated interior wall of a nanopipette tip. With backfilled pairing aptamers, the engineered nanopipette is then used for probing intracellular ATP via the ATP-dependent linkage of the split aptamers. Due to the higher surface charge density from the aptamer assembly, the nanosensor would exhibit an enhanced rectification signal. Besides, this ATP-responsive nanopipette tool possesses excellent selectivity and stability as well as high recyclability. This work provides a practical single-cell nanosensor capable of intracellular ATP analysis. More generally, integrated with other split recognition elements, the proposed mechanism could serve as a viable basis for addressing many other important biological species.
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http://dx.doi.org/10.1021/acs.analchem.0c04628DOI Listing
January 2021

Segmentation Method for Ship-Radiated Noise Using the Generalized Likelihood Ratio Test on an Ordinal Pattern Distribution.

Entropy (Basel) 2020 Mar 25;22(4). Epub 2020 Mar 25.

School of Marine Science and Technology, Northwestern Polytechnical University, Xi'an 710072, China.

Due to the diversity of ship-radiated noise (SRN), audio segmentation is an essential procedure in the ship statuses/categories identification. However, the existing segmentation methods are not suitable for the SRN because of the lack of prior knowledge. In this paper, by a generalized likelihood ratio (GLR) test on the ordinal pattern distribution (OPD), we proposed a segmentation criterion and introduce it into single change-point detection (SCPD) and multiple change-points detection (MCPD) for SRN. The proposed method is free from the acoustic feature extraction and the corresponding probability distribution estimation. In addition, according to the sequential structure of ordinal patterns, the OPD is efficiently estimated on a series of analysis windows. By comparison with the Bayesian Information Criterion (BIC) based segmentation method, we evaluate the performance of the proposed method on both synthetic signals and real-world SRN. The segmentation results on synthetic signals show that the proposed method estimates the number and location of the change-points more accurately. The classification results on real-world SRN show that our method obtains more distinguishable segments, which verifies its effectiveness in SRN segmentation.
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http://dx.doi.org/10.3390/e22040374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516847PMC
March 2020

Bayesian Discriminant Analysis of Yogurt Products Based on Raman Spectroscopy.

J AOAC Int 2020 Sep;103(5):1435-1439

Zhejiang Gongshang University, School of Management and E-Business, Hangzhou, Zhejiang 310018, The People's Republic of China.

Background: The quality discrimination of dairy products is an important basis on which to achieve quality assurance.

Objective: Taking the discriminant analysis of brand yogurt products as an example, a new rapid discriminant method can be constructed.

Method: The first three principal components were selected as the pattern vectors of the samples. Then, at random, 75% of the samples were collected as a training set, and their mean values and covariance matrices were calculated to construct a Gauss Bayesian discriminant model. The remaining 25% of samples were employed as a test set, and the pattern vectors of each sample were input into the above model. Next, the posterior probability of each sample in relation to each category could be obtained. Results: The category corresponding to the maximum posterior probability as the brand classification of each sample was defined.

Conclusions: We constructed a Gauss Bayesian discriminant model to discriminate these different yogurt products after the principal component feature extraction of Raman properties. The results indicate the rationality and wide application prospects of this approach.

Highlights: A fast dairy product discriminant method based on Gauss Bayesian model and Raman spectroscopy was established.
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http://dx.doi.org/10.1093/jaoacint/qsaa039DOI Listing
September 2020

[Quantitative analysis of the effects of different stimuli on the contraction of gastrocnemius and specimen].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2020 Jul;36(4):379-384

Department of Pathophysiology, Shantou University Medical College, Shantou 515041, China.

Objective: To quantitatively analyze the effects of direct and indirect stimuli on the contraction of gastrocnemius and specimen by self-programming.

Methods: All specimens were divided into four groups: indirect stimuli on specimen in vivo group (=12), direct stimuli on specimen in vivo group (=8), indirect stimuli on specimen in vitro group (=12), direct stimuli on specimen in vitro group (=8). Indirect stimuli ( sciatic nerve) and direct stimuli (acupuncture needle piercing into gastrocnemius) (stimuli starting from 0 V, cycle 3 s, increment 0.02 V, 150 times) were acted on and sciatic nerve gastrocnemius muscle specimen respectively. The effects of electric intensity on the contraction of gastrocnemius were recorded by the experimental system of BL-420F. The data were processed and analyzed by the help of self-programming, to quantitatively obtain key parameters for a single contraction.

Results: ① For in vivo specimen, compared with direct stimuli, effects of indirect stimuli were as follows: the threshold intensity, half-intensity and maximal intensity of the specimen were smaller (<0.05); the amplitude was larger, the contraction period was longer, and the rising slope was smaller (<0.05). ②For in vitro specimen, compared with direct stimuli, effects of indirect stimuli were as follows: the threshold intensity, half-intensity and maximal intensity of indirect stimuli were smaller (<0.05); the amplitude was larger, the contraction period was longer, and the rising slope was smaller (<0.05). ③Compared with in vitro specimen, there was no significant difference among all the above parameters of in vivo specimen, with either direct or indirect stimuli (>0.05).

Conclusion: There is no significant difference in the features of single contraction between in vivo and in vitro specimen with either direct or indirect stimuli. However, indirect stimuli can trigger gastrocnemius to produce single contraction more easily than direct stimuli, and the amplitude is larger than that of direct stimuli.
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http://dx.doi.org/10.12047/j.cjap.5976.2020.081DOI Listing
July 2020

Using a partial differential equation with Google Mobility data to predict COVID-19 in Arizona.

Math Biosci Eng 2020 07;17(5):4891-4904

School of Human Evolution and Social Change, Arizona State University, Tempe, AZ 85287, USA.

The outbreak of COVID-19 disrupts the life of many people in the world. The state of Arizona in the U.S. emerges as one of the country's newest COVID-19 hot spots. Accurate forecasting for COVID-19 cases will help governments to implement necessary measures and convince more people to take personal precautions to combat the virus. It is difficult to accurately predict the COVID- 19 cases due to many human factors involved. This paper aims to provide a forecasting model for COVID-19 cases with the help of human activity data from the Google Community Mobility Reports. To achieve this goal, a specific partial differential equation (PDE) is developed and validated with the COVID-19 data from the New York Times at the county level in the state of Arizona in the U.S. The proposed model describes the combined effects of transboundary spread among county clusters in Arizona and human activities on the transmission of COVID-19. The results show that the prediction accuracy of this model is well acceptable (above 94%). Furthermore, we study the effectiveness of personal precautions such as wearing face masks and practicing social distancing on COVID-19 cases at the local level. The localized analytical results can be used to help to slow the spread of COVID- 19 in Arizona. To the best of our knowledge, this work is the first attempt to apply PDE models on COVID-19 prediction with the Google Community Mobility Reports.
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http://dx.doi.org/10.3934/mbe.2020266DOI Listing
July 2020

PKM2-c-Myc-Survivin Cascade Regulates the Cell Proliferation, Migration, and Tamoxifen Resistance in Breast Cancer.

Front Pharmacol 2020 8;11:550469. Epub 2020 Sep 8.

Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.

The M2 isoform of pyruvate kinase (PKM2), as a key glycolytic enzyme, plays important roles in tumorigenesis and chemotherapeutic drug resistance. However, the intricate mechanism of PKM2 as a protein kinase regulating breast cancer progression and tamoxifen resistance needs to be further clarified. Here, we reported that PKM2 controls the expression of survivin by phosphorylating c-Myc at Ser-62. Functionally, PKM2 knockdown suppressed breast cancer cell proliferation and migration, which could be rescued by overexpression of survivin. Interestingly, we found that the level of PKM2 expression was upregulated in the tamoxifen resistant breast cancer cells MCF-7/TAMR, and knockdown of PKM2 sensitized the cells to 4-hydroxytamoxifen (4OH-T). In addition, the elevated level of PKM2 correlates with poor relapse-free survival in breast cancer patients treated with tamoxifen. Overall, our findings demonstrated that PKM2-c-Myc-survivin cascade regulated the proliferation, migration and tamoxifen resistance of breast cancer cells, suggesting that PKM2 represents a novel prognostic marker and an attractive target for breast cancer therapeutics, and that PKM2 inhibitor combined with tamoxifen may be a promising strategy to reverse tamoxifen resistance in breast cancer patients.
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http://dx.doi.org/10.3389/fphar.2020.550469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506054PMC
September 2020

The flavonoid-rich ethanolic extract from the green cocoon shell of silkworm has excellent antioxidation, glucosidase inhibition, and cell protective effects .

Food Nutr Res 2020 14;64. Epub 2020 Aug 14.

Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, Dushuhu Higher Edu. Town, Suzhou, P R China.

The green cocoon shell of a novel variety of silkworm, is rich in two types of quercetin and kaempferol flavonoids. The aim of this study was to identify these flavonoids in the ethanolic extract (EE) from green cocoons and develop EE applications in healthy foods. The experimental results indicated that the amount of total amino acids in EE was 27.06%. The flavonoids in EE are presented in quercetin and kaempferol glycosides. The total amount of the two aglycones was 33.42 ± 0.08 mg/g. The IC values of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 1,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonicacid) (ABTS), and hydroxyl radical scavenging abilities were 296.95 ± 13.24 μg/mL, 94.31 ± 9.13 μg/mL, and 9.21 ± 0.15 mg/mL, respectively. The IC values of the inhibitory activities of α-amylase and α-glucosidase were 37.57 ± 6.45 μg/mL and 212.69 ± 22.94 μg/mL, respectively. EE also reduced the level of reactive oxygen species (ROS) and oxidative stress in L02 cells induced by high glucose levels. It also effectively decreased the content of 8-hydroxyl deoxyguanosine (8-OHdG), nuclear factor κB (NF-κB), and tumour necrosis factor alpha (TNF-α) in cells with a good dose effect. These results clearly indicated that the flavonoid-rich EE with excellent antioxidant and glucosidase inhibition abilities significantly reduced the damage to cells caused by oxidative stress and inflammatory reactions. It is suggested that EE might serve as useful functional foods for the treatment of related diseases induced by oxidative stress such as diabetes mellitus.
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http://dx.doi.org/10.29219/fnr.v64.1637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478120PMC
August 2020

The Effects of P75NTR on Learning Memory Mediated by Hippocampal Apoptosis and Synaptic Plasticity.

Curr Pharm Des 2021 ;27(4):531-539

State Key Laboratory of Trauma, Burns and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, China.

Neurological diseases bring great mental and physical torture to the patients, and have long-term and sustained negative effects on families and society. The attention to neurological diseases is increasing, and the improvement of the material level is accompanied by an increase in the demand for mental level. The p75 neurotrophin receptor (p75NTR) is a low-affinity neurotrophin receptor and involved in diverse and pleiotropic effects in the developmental and adult central nervous system (CNS). Since neurological diseases are usually accompanied by the regression of memory, the pathogenesis of p75NTR also activates and inhibits other signaling pathways, which has a serious impact on the learning and memory of patients. The results of studies shown that p75NTR is associated with LTP/LTD-induced synaptic enhancement and inhibition, suggest that p75NTR may be involved in the progression of synaptic plasticity. And its proapoptotic effect is associated with activation of proBDNF and inhibition of proNGF, and TrkA/p75NTR imbalance leads to pro-survival or proapoptotic phenomena. It can be inferred that p75NTR mediates apoptosis in the hippocampus and amygdale, which may affect learning and memory behavior. This article mainly discusses the relationship between p75NTR and learning memory and associated mechanisms, which may provide some new ideas for the treatment of neurological diseases.
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http://dx.doi.org/10.2174/1381612826666200916145142DOI Listing
April 2021

Data Mining Study on Prescription Patterns of Different Dosage Forms of Chinese Herbal Medicines for Treating and Improving Immune-Inflammatory Indices in Patients with Rheumatoid Arthritis.

Chin J Integr Med 2020 Aug 22. Epub 2020 Aug 22.

Department of Internal Medicine, Bozhou Hospital of Chinese Medicine, Bozhou, Anhui Province, 236800, China.

Objective: To explore the prescription patterns of different dosage forms of Chinese herbal medicines (CHMs) for the treatment of rheumatoid arthritis (RA) and their effects on immune-inflammatory indices.

Methods: Clinical data were collected from patients with RA in 4 hospitals (3 Class A comprehensive hospitals and 1 Class B comprehensive hospital) in Anhui Province, China, from August 2012 to June 2018 via the electronic medical record gathering system. Following extraction of prescription information, each prescribed herb was quantified and standardized according to the knowledge base to establish a database of RA treatment formulae. The medical records were divided into the granules group and decoction pieces group. Core herbs and their combination patterns were obtained from the two groups of cases using Liquorice software. Changes in immune-inflammatory and hepatic and renal function indices were compared between the two groups using SPSS 23.0 software. The Aprior module of SPSS Clementine 11.1 software was applied to analyse the correlation between CHMs and improvement in indices. Finally, the ORACLE 10 g tool was used to evaluate the random walk model of the immune-inflammatory indices between the two groups.

Results: (1) We retrospectively analysed 35,898 prescriptions for 6,829 patients with RA who received CHM treatment. There were 3,816 patients in the granules group and 3,013 in the decoction pieces group. (2) The core herbs were Pi (Spleen)-strengthening and dampness-resolving drugs, blood-activating and stasis-resolving drugs, wind/dampness-dispelling drugs and heat-clearing and detoxifying drugs. (3) Both dosage forms could improve immune-inflammatory indices in RA patients, with similar efficacy and no influence on hepatic or renal function. (4) Herba Siegesbeckiae and Oldenlandia had a stronger association with immune-inflammatory indices in the two groups. (5) The immune-inflammatory indices showed obvious improvement after treatment with granules and decoction pieces of CHMs, and there were long range correlations between the comprehensive evaluation indices and interventions.

Conclusions: The principal CHM treatment methods for RA in four hospitals in Anhui Province are strengthening Pi and resolving dampness, activating blood and resolving stasis, dispelling wind/dampness and clearing heat. Granules and decoction pieces of CHMs have similar efficacy in improving immune-inflammatory indices in RA patients and could be used as treatment options for RA.
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http://dx.doi.org/10.1007/s11655-020-3480-1DOI Listing
August 2020

Sishen Pill Treatment of DSS-Induced Colitis Regulating Interaction With Inflammatory Dendritic Cells and Gut Microbiota.

Front Physiol 2020 9;11:801. Epub 2020 Jul 9.

Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.

Sishen Pill (SSP) is a typical prescription in the pharmacopeia of traditional Chinese medicine (TCM), and is usually used to treat inflammatory bowel disease (IBD). It is known that inflammatory dendritic cells (DCs) and imbalance of gut microbiota play significant roles in the pathogenesis of IBD. However, it is not clear whether SSP can treat IBD by regulating interaction of DCs and gut microbiota. In the present study, the levels of inflammatory DCs and gut microbiota were analyzed by flow cytometry and 16S rDNA analysis. SSP relieved the pathological damage to the colon of mice with colitis induced by dextran sodium sulfate (DSS). As typical indicators of inflammatory DCs, the levels of CD11cCD103E-cadherin cells and pro-inflammatory cytokines [interleukin (IL)-1β, -4, -9, and -17A] were decreased in mice with colitis treated by SSP for 10 days. Simultaneously, the gut microbiota composition was regulated, and beneficial bacteria were increased and pathogenic bacteria were reduced. The results indicated that SSP regulated the interaction between inflammatory DCs and gut microbiota to treat DSS-induced colitis.
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http://dx.doi.org/10.3389/fphys.2020.00801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381313PMC
July 2020

Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.

J Ethnopharmacol 2020 Nov 30;262:113211. Epub 2020 Jul 30.

Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, Jiangxi Province, China; Formula-Pattern Research Center of Jiangxi, Nanchang, 330004, Jiangxi Province, China. Electronic address:

Ethnopharmacological Relevance: As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years. However, its mechanism of action remains unclear.

Aim Of The Study: METHODS: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d. In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis. Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.

Results: The pathological damage was attenuated and expression of proinflammatory cytokines was decreased. While the diversity of intestinal microflora was regulated, the relative abundance of Actinobacteria, and Sphingobacteriia was modified. Meanwhile, the level of CD4CD25Foxp3 and PD-L1 Treg cells improved. These changes maintained a positive correlation which was analyzed statistically. Our results also showed that ZJP inhibited activation of the PI3K/Akt signaling pathway.

Conclusions: ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
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http://dx.doi.org/10.1016/j.jep.2020.113211DOI Listing
November 2020

Effect of Sishen Pill on Memory T Cells From Experimental Colitis Induced by Dextran Sulfate Sodium.

Front Pharmacol 2020 2;11:908. Epub 2020 Jul 2.

Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.

Immune memory has a protective effect on the human body, but abnormal immune memory is closely related to the occurrence and development of autoimmune diseases including inflammatory bowel disease (IBD). Sishen Pill (SSP) is a classic prescription of traditional Chinese medicine, which is often used to treat chronic colitis, but it is not clear whether SSP can alleviate experimental colitis by remodeling immune memory. In the present study, the therapeutic effect of SSP on chronic colitis induced by dextran sulfate sodium (DSS) was evaluated by colonic length, colonic weight index, macroscopic and microscopic scores, and pathological observation. The cytokine levels were tested by enzyme-linked immunosorbent assay (ELISA); the percentages of central memory T (Tcm) and effector memory T (Tem) cells were analyze\d by flow cytometry; and activation of phosphoinositide 3-kinase (PI3K)/Akt signaling proteins was measured by western blotting. After 7-days' treatment, SSP alleviated DSS-induced colitis, which was demonstrated by decreased colonic weight index, colonic weight, histopathological injury scores, restored colonic length, gradual recovery of colonic mucosa, and lower levels of interleukin (IL)-2, IL-7, IL-12, and IL-15, while SSP increased IL-10 expression. SSP obviously regulated the quantity and subpopulation of Tcm and Tem cells. Furthermore, SSP markedly inhibited activation of PI3K, Akt, phospho-Akt, Id2, T-bet, forkhead box O3a, Noxa, and C-myc proteins in the PI3K/Akt signaling pathway and activated Rictor, Raptor, tuberous sclerosis complex (TSC)1, TSC2, phospho-AMP-activated kinase (AMPK)-, AMPK-, eukaryotic translation initiation factor 4E-binding protein 2, kinesin family member 2a, and 70-kDa ribosomal protein S6 kinase. These results indicate that SSP effectively controls Tem cells in the peripheral blood to relieve experimental colitis induced by DSS, which were potentially related with inhibiting the PI3K/Akt signaling pathway.
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http://dx.doi.org/10.3389/fphar.2020.00908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343851PMC
July 2020

Psychological symptoms in anophthalmic patients wearing ocular prosthesis and related factors.

Medicine (Baltimore) 2020 Jul;99(29):e21338

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Lab, Beijing, China.

Anophthalmic patients not only cause obvious functional deficits and facial deformities, but lead to poor psychological outcomes, although prosthesis wearing can offer improvements in psychological well-being to some extent. The study aimed to comprehensively evaluate the psychological symptoms and analyze related factors in anophthalmic patients wearing ocular prosthesis.Total of 150 anophthalmic patients and 120 control subjects were included in this cross-sectional study. Baseline characteristics survey and the symptom checklist-90 scale were completed by all participants to assess the psychological symptoms and analyze their related factors by multivariate analysis.The anophthalmic patients exhibited the increased levels of somatization, depression, anxiety, and hostility compared with control subjects. The most prominent symptom was hostility with the median score of 1.20. Female patients presented with higher somatization, depression, anxiety, and hostility. Marital status single was positively associated with depression, anxiety, and hostility symptoms. Lower education and cause of enucleation were related to higher levels of hostility.Anophthalmic patients wearing ocular prosthesis presented with more prominent hostility and somatization besides its higher depression and anxiety symptoms. The findings suggest that for female single anophthalmic patients with low education, especially caused by trauma, timely psychological assessment and intervention should be provided to avoid undesirable consequences.
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http://dx.doi.org/10.1097/MD.0000000000021338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7373533PMC
July 2020