Publications by authors named "Hai-Dan Zhao"

4 Publications

  • Page 1 of 1

Cognitive impairment and associated risk factors in older adult hemodialysis patients: a cross-sectional survey.

Sci Rep 2020 07 27;10(1):12542. Epub 2020 Jul 27.

Department of Nephrology, Chinese PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing, 100853, China.

The clinical epidemiological features of cognitive impairment in Chinese older adult patients undergoing hemodialysis are not clear, we aimed to identify the extent and patterns of cognitive impairment among those patients. We conducted a cross-sectional study of 613 hemodialysis patients aged 50 to 80 from 11 centers in Beijing. A neuropsychological battery of 11 tests covering domains of attention/processing speed, executive function, memory, language, and visuospatial function was applied, patients were classified as none, mild, or major cognitive impairment according to the fifth version of the Diagnostic and Statistical Manual of Mental Disorders criteria for cognitive impairment. Compared with Chinese population norms, 37.2% of the participants had mild cognitive impairment, 43.7% had major cognitive impairment. Memory and language were the most severe impaired domains in the mild cognitive impairment group, attention and visuospatial function domains were the most serious impaired domains in the major cognitive impairment group. Concomitant impairment across multiple cognitive domains was common. Factors associated with major cognitive impairment included age, education level, history of stroke and hypertension, dialysis vintage, and single-pool Kt/V. There is a high frequency of cognitive impairment in Chinese older adult hemodialysis patients, with varying severity and concomitant impairment across multiple domains.
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http://dx.doi.org/10.1038/s41598-020-69482-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385128PMC
July 2020

Elevated serum cartilage oligomeric matrix protein and the metalloproteinase-ADAMTS7 levels are associated with vascular calcification in maintenance hemodialysis patients.

Semin Dial 2020 07 22;33(4):322-329. Epub 2020 May 22.

Department of Nephrology, Peking University Shougang Hospital, Beijing, China.

Background: Vascular calcification is common in maintenance hemodialysis (HD) patients. Recent studies showed that cartilage oligomeric matrix protein (COMP) could protect blood vessels from calcification, but the role of ADAMTS7 was opposite. We aimed to investigate the relationship between serum COMP, ADAMTS7 levels and vascular calcification scores in HD patients.

Methods: Serum COMP and ADAMTS7 levels were tested by ELISA and we compared calcification scores between high and low COMP groups, also between high and low ADAMTS7 groups. We also investigated the differences of serum COMP and ADAMTS7 levels between mild and severe vascular calcification groups. The relationship between serum COMP and ADAMTS7 levels was analyzed in the end.

Results: Serum COMP and ADAMTS7 levels were both higher in HD patients than in control (29.63 vs 14.23 ng/mL, P = .002, 11.12 vs 2.40 ng/mL, P = .005). Serum COMP levels in severe vascular calcification (VC) group were higher than in mild VC (43.13 ± 28.77 vs 26.75 ± 18.22 ng/mL, P = .010). Serum ADAMTS7 levels were positively correlated with radial and digital arteries (small arteries) calcification scores (r = .249, P = .033). And serum COMP and ADAMTS7 levels were positively correlated (r = .348, P = .026).

Conclusions: Serum COMP and ADAMTS7 levels were probably associated with vascular calcification scores in HD respectively.
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http://dx.doi.org/10.1111/sdi.12885DOI Listing
July 2020

[Expression of PKD1 and PKD2 transcripts and proteins and its significance in different types of kidney tissues and kidney lines].

Zhonghua Bing Li Xue Za Zhi 2005 Oct;34(10):646-9

Kidney Disease Center of PLA, Second Military Medical University, Changzhen Hospital, Shanghai, China.

Objective: To investigate the expression and function of PKD1 and PKD2 in different kidney tissues and cell lines.

Methods: Immunoprecipitation, Western blotting, In situ hybridization and immunohistochemical staining methods were used to observe the expression of PKD1 mRNA and PKD2 mRNA and their protein abundance in different kidney tissues and cell lines.

Results: Coordinate expressions of PKD1 and PKD2 were found in all kidney tissues and cell lines. Distribution of PKD1 mRNA and PKD2 mRNA and their protein polycystin-1 and polycystin-2 in normal human adult kidney tissue were mainly expressed in the medullary collecting ducts and distal tubules. Positive staining was also found in the majority of cyst-lining epithelial cells of PKD1 cystic kidney tissue, PKD1 cyst-lining epithelia cell line and LLC-PK1. The expression level of them in cystic epithelia of ADPKD kidney tissue was much higher than that in adult renal tubules (P < 0.01).

Conclusions: Similar expression pattern of PKD1 and PKD2 and their different tissue distribution in different kidney tissues show that the molecular mutuality of PC-1 and PC-2 might be the base of their functional correlation. Polycystins might play an important role in the maintenance of tubular architecture.
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October 2005

[Preparation and characterization of monoclonal antibody against N-terminal domain of polycystin 1].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2004 Mar;20(2):186-90

Division of Nephrology, Kidney Disease Center of PLA, Second Military Medical University, Changzheng Hospital, Shanghai 200003, China.

Aim: To prepare and identify monoclonal antibody (mAb) against N-terminal domain of polycystin 1.

Methods: Total RNA was extracted from kidney tissue of a healthy man. Gene sequence encoding polycystin 1 N-terminal domain was amplified by one-step RT-PCR. The target gene was inserted into prokaryotic expression vector pQE30 and transformed into competent cells E. coli M15. The fusion protein was expressed under IPTG induction and purified by affinity chromatography. The purified fusion protein was then used to immunize BALB/c mice. The splenocytes from immunized mice were fused with myeloma cells Sp2/0 by PEG 4000 mediator method and the hybridomas were selected in HAT medium. The hybridoma clones secreting mAb against polycystin 1 amino-terminal domain were detected by ELISA and cloned by limiting dilution. The specificity of mAb against polycystin 1 N-terminal domain was verified by ELISA and Western blot.

Results: cDNA encoding polycystin 1 extracellular region was obtained. Fusion protein of polycystin 1 N-terminal domain were expressed in pQE30 expression system. The relative molecular masses (Mr) of the two fusion proteins were 19,800 and 18,900, respectively. One hybridoma cell 7B1 secreting specific mAb was obtained. Western blot analysis showed that the mAb reacted strongly and specifically to polycystin 1 N-terminal domain.

Conclusion: polycystin 1 N-terminal fusion proteins have been expressed in E.coli M15. Anti-fusion protein mAb with antigen-binding activity has been prepared successfully.
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March 2004