Publications by authors named "Hai Long"

140 Publications

Exertional rhabdomyolysis in newly enrolled cadets of a military academy.

Muscle Nerve 2021 Jun 20. Epub 2021 Jun 20.

Medical School of Chinese PLA, Beijing, China.

Introduction/aims: Exertional rhabdomyolysis (ER) often occurs during prolonged intense exercise in hot environments, posing a threat to the health of military personnel. This study aimed to investigate possible risk factors for ER and provide further empirical data for prevention and clinical treatment strategies.

Methods: A retrospective investigation of 116 concurrent ER cases was conducted. Conditional logistic regression analyses were performed to assess the association between each potential risk (or protective) factor and ER. The clinical characteristics of the 71 hospitalized patients were analyzed descriptively.

Results: After screening, the following variables significantly increased the risk of ER: shorter length of service (recruits, odds ratios [OR] 7.49, 95% confidence interval [CI] 2.58-21.75), higher body mass index (BMI, OR 1.14, 95%CI 1.03-1.26), lack of physical exercise in the last half year (< once a month, OR 3.20, 95%CI 1.08-9.44), and prior heat injury (OR 2.94, 95%CI 1.26-6.89). Frequent fruit consumption (OR 0.57, 95%CI 0.33-0.99), active hydration habit (OR 0.37, 95%CI 0.20-0.67), water replenishment ≥2 L on the training day (OR 0.15, 95%CI 0.05-0.45), and water replenishment ≥500 mL within 1 h before training (OR 0.33, 95%CI 0.12-0.88) significantly decreased the risk of ER. Of the 71 hospitalized patients, 41 (57.7%) were diagnosed with hypokalemia on admission.

Discussion: In military training, emphasis should be placed on incremental adaptation training prior to more intense training, and close attention should be given to overweight and previously sedentary recruits. Fluid replenishment before exercise, increased fruit intake, and proper potassium supplementation may help prevent ER.
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http://dx.doi.org/10.1002/mus.27355DOI Listing
June 2021

Carbohydrate-binding module -mannosylation alters binding selectivity to cellulose and lignin.

Chem Sci 2020 Aug 19;11(34):9262-9271. Epub 2020 Aug 19.

Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100050 China

Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies. In this study, we examine the impact of -glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases. Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates. We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin. In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites. Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity. Taken together, this work helps lay the groundwork for future exploitation of glycoengineering as a tool to improve the performance of industrial enzymes.
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http://dx.doi.org/10.1039/d0sc01812kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163390PMC
August 2020

PAL-mediated SA biosynthesis pathway contributes to nematode resistance in wheat.

Plant J 2021 May 11. Epub 2021 May 11.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.

The pathogen cereal cyst nematode (CCN) is deleterious to Triticeae crops and is a threat to the global crop yield. Accession no. 1 of Aegilops variabilis, a relative of Triticum aestivum (bread wheat), is highly resistant to CCN. Our previous study demonstrated that the expression of the phenylalanine ammonia lyase (PAL) gene AevPAL1 in Ae. variabilis is strongly induced by CCN. PAL, the first enzyme of phenylpropanoid metabolism, is involved in abiotic and biotic stress responses. However, its role in plant-CCN interaction remains unknown. In the present study, we proved that AevPAL1 helps to confer CCN resistance through affecting the synthesis of salicylic acid (SA) and downstream secondary metabolites. The silencing of AevPAL1 increased the incidence of CCN infection in roots and decreased the accumulation of SA and phenylalanine (Phe)-derived specialized metabolites. The exogenous pre-application of SA also improved CCN resistance. Additionally, the functions of PAL in phenylpropanoid metabolism correlated with tryptophan decarboxylase (TDC) functioning in tryptophan metabolism pathways. The silencing of either AevPAL1 or AevTDC1 exhibited a concomitant reduction in the expression of both genes and the contents of metabolites downstream of PAL and TDC. These results suggested that AevPAL1, possibly in coordination with AevTDC1, positively contributes to CCN resistance by altering the downstream secondary metabolites and SA content in Ae. variabilis. Moreover, AevPAL1 overexpression significantly enhanced CCN resistance in bread wheat and did not exhibit significant negative effects on yield-related traits, suggesting that AevPAL1 is valuable for the genetic improvement of CCN resistance in bread wheat.
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http://dx.doi.org/10.1111/tpj.15316DOI Listing
May 2021

Identification and candidate gene mining of HvSS1, a novel qualitative locus on chromosome 6H, regulating the uppermost internode elongation in barley (Hordeum vulgare L.).

Theor Appl Genet 2021 May 3. Epub 2021 May 3.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, Sichuan, People's Republic of China.

Key Message: A novel qualitative locus regulating the uppermost internode elongation of barley was identified and mapped on 6H, and the candidate gene mining was performed by employing various barley genomic resources. The stem of grass crops, such as barley and wheat, is composed of several interconnected internodes. The extent of elongation of these internodes determines stem height, and hence lodging, canopy architecture, and grain yield. The uppermost internode (UI) is the last internode to elongate. Its elongation contributes largely to stem height and facilitates spike exsertion, which is crucial for final grain yield. Despite the molecular mechanism underlying regulation of UI elongation was extensively investigated in rice, little is known in barley. In this study, we characterized a barley spontaneous mutant, Sheathed Spike 1 (SS1), showing significantly shortened UI and sheathed spike (SS). The extension of UI parenchyma cell in SS1 was significantly suppressed. Exogenous hormone treatments and RNA-seq analysis indicated that the suppression of UI elongation is possibly related to insufficient content of endogenous bioactive gibberellin. Genetic analysis showed that SS1 is possibly controlled by a qualitative dominant nuclear factor. Bulked segregant analysis and further molecular marker mapping identified a novel major locus, HvSS1, in a recombination cold spot expanding 173.44-396.33 Mb on chromosome 6H. The candidate gene mining was further conducted by analyzing sequence differences, spatiotemporal expression patterns, and variant distributions of genes in the candidate interval by employing various barley genomic resources of worldwide collections of barley accessions. This study made insight into genetic control of UI elongation in barley and laid a solid foundation for further gene cloning and functional characterization. The results obtained here also provided valuable information for similar research in wheat.
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http://dx.doi.org/10.1007/s00122-021-03837-8DOI Listing
May 2021

Identification and Validation of a Novel Locus Controlling Spikelet Number in Bread Wheat ( L.).

Front Plant Sci 2021 26;12:611106. Epub 2021 Feb 26.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

Spikelet number is an important target trait for wheat yield improvement. Thus, the identification and verification of novel quantitative trait locus (QTL)/genes controlling spikelet number are essential for dissecting the underlying molecular mechanisms and hence for improving grain yield. In the present study, we constructed a high-density genetic map for the Kechengmai1/Chuanmai42 doubled haploid (DH) population using 13,068 single-nucleotide polymorphism (SNP) markers from the Wheat 55K SNP array. A comparison between the genetic and physical maps indicated high consistence of the marker orders. Based on this genetic map, a total of 27 QTLs associated with total spikelet number per spike (TSN) and fertile spikelet number per spike (FSN) were detected on chromosomes 1B, 1D, 2B, 2D, 3D, 4A, 4D, 5A, 5B, 5D, 6A, 6B, and 7D in five environments. Among them, five QTLs on chromosome 2D, 3D, 5A, and 7D were detected in multiple environments and combined QTL analysis, explaining the phenotypic variance ranging from 3.64% to 23.28%. Particularly, for TSN and FSN [phenotypic variation explained (PVE) = 5.97-23.28%, limit of detection (LOD) = 3.73-18.51] is probably a novel locus and located in a 4.5-cM interval on chromosome arm 3DL flanking by the markers and This QTL was further validated in other two populations with different genetic backgrounds using the closely linked Kompetitive Allele-Specific PCR (KASP) marker . The results indicated that significantly increased the TSN (5.56-7.96%) and FSN (5.13-9.35%), which were significantly correlated with grain number per spike (GNS). We also preliminary analyzed the candidate genes within this locus by sequence similarity, spatial expression patterns, and collinearity analysis. These results provide solid foundation for future fine mapping and cloning of . The developed and validated KASP markers could be utilized in molecular breeding aiming to increase the grain yield in wheat.
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http://dx.doi.org/10.3389/fpls.2021.611106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952655PMC
February 2021

A Review of Key Technologies and Trends in the Development of Integrated Heating and Power Systems in Agriculture.

Entropy (Basel) 2021 Feb 23;23(2). Epub 2021 Feb 23.

College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China.

Petroleum agriculture, characterized by mechanization and chemistry, is developing rapidly in China. However, petroleum agriculture has not only brought food safety problems, but also caused great obstacles to the sustainable development of society. In view of the disadvantages of oil agriculture, we provide an upgrading plan for energy systems in agriculture. This work can help reduce carbon emissions and improve food security. We introduce the most advanced technologies in Chinese agricultural development and the technical scope includes new agricultural energy power generation, agricultural energy use and the safe operation of agricultural energy systems. We describe the detailed data of agricultural bioenvironmental and energy engineering to clarify the level of agricultural energy efficiency in China. The overall conclusion of this paper is that the deep integration of agriculture and energy internet has become the development trend of agricultural energy systems.
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http://dx.doi.org/10.3390/e23020260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926571PMC
February 2021

A deep learning-based smartphone platform for cutaneous lupus erythematosus classification assistance: Simplifying the diagnosis of complicated diseases.

J Am Acad Dermatol 2021 Feb 19. Epub 2021 Feb 19.

Department of Dermatology, Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, China; Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Najing, China. Electronic address:

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http://dx.doi.org/10.1016/j.jaad.2021.02.043DOI Listing
February 2021

UHRF1 downregulation promotes T follicular helper cell differentiation by increasing BCL6 expression in SLE.

Clin Epigenetics 2021 Feb 10;13(1):31. Epub 2021 Feb 10.

Department of Dermatology, Second Xiangya Hospital, Central South University, #139 Renmin Middle Road, Changsha, 410011, Hunan, China.

Background: Transcription factor B cell lymphoma 6 (BCL6) is a master regulator of T follicular helper (Tfh) cells, which play a crucial role in the pathogenesis of systemic lupus erythematosus (SLE). However, the mechanisms by which BCL6 expression is regulated are poorly understood. Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is an important epigenetic factor that regulates DNA methylation and histone modifications. In the present study, we assessed whether UHRF1 can regulate BCL6 expression and influence the differentiation and proliferation of Tfh cells.

Results: Compared to healthy controls, the mean fluorescence intensity of UHRF1 (UHRF1-MFI) in Tfh cells from SLE patients was significantly downregulated, whereas that of BCL6 (BCL6-MFI) was significantly upregulated. In vitro, UHRF1 knockdown led to BCL6 overexpression and promoted Tfh cell differentiation. In contrast, UHRF1 overexpression led to BCL6 downregulation and decreased Tfh cell differentiation. In vivo, conditional UHRF1 gene knockout (UHRF1-cKO) in mouse T cells revealed that UHRF1 depletion can enhance the proportion of Tfh cells and induce an augmented GC reaction in mice treated with NP-keyhole limpet hemocyanin (NP-KLH). Mechanistically, UHRF1 downregulation can decrease DNA methylation and H3K27 trimethylation (H3K27me3) levels in the BCL6 promoter region of Tfh cells.

Conclusions: Our results demonstrated that UHRF1 downregulation leads to increased BCL6 expression by decreasing DNA methylation and H3K27me3 levels, promoting Tfh cell differentiation in vitro and in vivo. This finding reveals the role of UHRF1 in regulating Tfh cell differentiation and provides a potential target for SLE therapy.
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http://dx.doi.org/10.1186/s13148-021-01007-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874639PMC
February 2021

No Longterm Severe Thyroid Dysfunction Seen In Patients With Preexisting Reduced Serum Tt3 Concentrations After A Single Large Dose Of Iodinated Contrast.

Endocr Pract 2020 Aug;26(8):840-845

From the Department of Cardiovascular Disease, Tianjin Chest Hospital, the Affiliated Hospital of Tianjin University and Nankai University, the Clinical College of Tianjin Medical University, Tianjin, China.

Objective: After an intravenous bolus injection of 100 mL of iodinated contrast agent (370 mgI/mL), the amount of iodine atoms entering the blood is tens of thousands of times the daily dose of iodine recommended by the World Health Organization. However, the effect of iodinated contrast in patients with nonthyroidal illness, manifested as reduced serum total triiodothyronine (TT3) concentrations, is unclear. We studied the effect of iodinated contrast on thyroid function and auto-antibodies in patients with reduced TT3 after diagnosis and treatment of coronary heart disease.

Methods: This was a prospective cohort study. One hundred and fifty-four stable angina pectoris patients with reduced TT3 and normal thyroid-stimulating hormone (TSH), free thyroxine (FT4), and reverse triiodothyronine (rT3) were enrolled from January, 2017, to June, 2018. All subjects had no history of thyroid dysfunction and had no recent infections, tumors, trauma, or other critical illnesses. Fourty-one patients underwent coronary angiography and 113 patients underwent coronary intervention.

Results: There were 6 patients (3.9%) with hypothyroidism and 30 patients (19.5%) developed subclinical hypothyroidism (SCHypo) on the first day after surgery. There were 6 patients (3.9%) with hypothyroidism, 6 patients (3.9%) with SCHypo, and 18 patients (11.7%) with subclinical hyperthyroidism (SCHyper) at the first month postsurgery. There were 23 patients (14.9%) with SCHyper and 6 patients (3.9%) with SCHypo at the sixth month after surgery. No patient with longterm severe thyroid dysfunction occurred during follow-up. The levels of free triiodothyronine, FT4, TT3, total thyroxine, and TSH showed statistically significant changes at 1 day, and 1, 3, and 6 months postoperative (P<.005). The level of rT3 showed no statistically significant change at 1, 3, and 6 months postoperative (P>.05). The levels of thyroglobulin antibody and thyroid peroxidase antibody decreased at 6 months postoperative (P<.001).

Conclusion: The risk of subclinical thyroid dysfunction and transient hypothyroidism occurred with a single large dose of iodinated contrast in the diagnosis and treatment of coronary heart disease, but no longterm severe thyroid dysfunction occurred. Patients with preoperative thyroid antibody elevation were more likely to have subclinical thyroid dysfunction after surgery.

Abbreviations: FT3 = free triiodothyronine; FT4 = free thyroxine; PCI = percutaneous coronary intervention; rT3 = reverse triiodothyronine; SCHyper = subclinical hyperthyroidism; SCHypo = subclinical hypothyroidism; TGAB = thyroglobulin antibody; TPOAB = thyroid peroxidase antibody; TT3 = total triiodothyronine; TT4 = total thyroxine; TSH = thyroid-stimulating hormone; WHO = World Health Organization.
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http://dx.doi.org/10.4158/EP-2019-0600DOI Listing
August 2020

Quantitative Trait Locus (QTLs) Mapping for Quality Traits of Wheat Based on High Density Genetic Map Combined With Bulked Segregant Analysis RNA-seq (BSR-Seq) Indicates That the Gene Is Related to Falling Number.

Front Plant Sci 2020 10;11:600788. Epub 2020 Dec 10.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

Numerous quantitative trait loci (QTLs) have been identified for wheat quality; however, most are confined to low-density genetic maps. In this study, based on specific-locus amplified fragment sequencing (SLAF-seq), a high-density genetic map was constructed with 193 recombinant inbred lines derived from Chuanmai 42 and Chuanmai 39. In total, 30 QTLs with phenotypic variance explained (PVE) up to 47.99% were identified for falling number (FN), grain protein content (GPC), grain hardness (GH), and starch pasting properties across three environments. Five genes closely adjacent to probably have effects on GPC. was the only one detected for GH with high PVE of 33.31-47.99% across the three environments and was assumed to be related to the nearest and genes. Three QTLs were identified for FN in at least two environments, of which had relatively higher PVE of 16.58-25.74%. The positive effect of for high FN was verified in a double-haploid population derived from Chuanmai 42 Kechengmai 4. The combination of these QTLs has a considerable effect on increasing FN. The transcript levels of and in were significantly different between low FN and high FN bulks, as observed through bulk segregant RNA-seq (BSR). These QTLs and candidate genes based on the high-density genetic map would be beneficial for further understanding of the genetic mechanism of quality traits and molecular breeding of wheat.
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http://dx.doi.org/10.3389/fpls.2020.600788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793810PMC
December 2020

[Preparation and quality evaluation of orodispersible film containing ginkgolide B novel nanosuspension lyophilized powder].

Zhongguo Zhong Yao Za Zhi 2020 Nov;45(22):5504-5510

Department of Pharmacy,Air Force Medical Center, PLA Beijing 100142, China.

To prepare a new dosage form that can improve the drug loading of the film--ginkgolide B nanosuspension lyophilized powder orodispersible film(GB-NS-LP-ODF) and to evaluate its quality. Firstly, ginkgolide B nanosuspension(GB-NS) was prepared by media milling method, and then ginkgolide B nanosuspension lyophilized powder(GB-NS-LP) was prepared with freeze-drying method. The mannitol was used as lyoprotectant and its dosage was also investigated. GB-NS-LP-ODF was prepared by solvent casting method and its formulation was screened by single factor test method and optimized by orthogonal test. The appearance, mechanical properties, content uniformity and in vitro dissolution of the optimized GB-NS-LP-ODF were investigated. The particle size of prepared GB-NS was about 201 nm, and the optimal dosage of mannitol was 8%. According to the optimal formula, the GB-NS-LP-ODF was prepared with GB-NS-LP 35.6%, PVA 0588 49.4%, PEG 400 10.7% and CMS-Na 4.3%, and completely disintegrated in about 30 s, and the particle size of reconstituted GB nanoparticles from ODF was about 210 nm. The film with smooth appearance and good mechanical properties was stable within 30 days and the content uniformity(A+2.2 S<15) conformed to the regulations. Scanning electron microscope(SEM) showed that GB-NS-LP-ODFs were evenly distributed and the particle size was about 200 nm. X-rays diffraction(XRD) showed that its crystallinity was significantly lower than that of GB raw drug and GB-ODF. The results of in vitro release test showed that the drug film was completely dissoluted within 10 minutes. These results indicated that nanosuspension lyophilized powder was prepared by freeze drying of nanosuspensions, and then loaded into the orodispersible film to effectively increase the drug loading of the ODF and have broad application prospects.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200819.306DOI Listing
November 2020

[Qualitative and quantitative analysis of quassinoid diterpenoids from Eurycoma longifolia by UPLC-Q-TOF-MS combined with UPLC-QQQ-MS/MS].

Zhongguo Zhong Yao Za Zhi 2020 Oct;45(19):4667-4676

Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology Fuzhou 350007, China.

In this work, a sensitive and efficient method was established and validated for qualitative and quantitative analysis of major quassinoid diterpenoids constituents from the extract of Eurycoma longifolia by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) and ultra-performance liquid chromatography coupled with a triple quadrupole mass spectrometry(UPLC-QQQ-MS/MS). The UPLC-Q-TOF-MS analysis was performed on an Agilent Eclipse Plus C_(18) RRHD(2.1 mm×100 mm,1.8 μm) column with acetonitrile and 0.1% formic acid water as mobile phase by gradient elution. The UPLC-QQQ-MS/MS analysis was performed on an Agilent Eclipse Plus C_(18) RRHD(2.1 mm×50 mm, 1.8 μm)column with acetonitrile and 0.1% formic acid water as mobile phase by gradient elution. The data was collected by electrospray ionization in positive mode. According to the contrast of the reference standards and the accurate masses of molecules, a total of 32 quassinoid diterpenoids in E. longifolia extract were identified by UPLC-Q-TOF-MS. For quantitative the linear range of 4 detected quassinoid diterpenoids were good(r≥0.999 6), and the overall recoveries ranged from 90.35% to 106.4%, with the RSD ranging from 1.8% to 3.6%. The method was accurate, reliable and efficient, and could comprehensively reflect the chemical constituents and content of E. longifolia, and could provide a reference for further elucidating its pharmacological basis and quality control.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200630.201DOI Listing
October 2020

Recent advances in understanding pathogenesis and therapeutic strategies of Systemic Lupus Erythematosus.

Int Immunopharmacol 2020 Dec 8;89(Pt A):107028. Epub 2020 Oct 8.

Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, China. Electronic address:

Systemic lupus erythematosus (SLE) is a multi-system-involving autoimmune disorder mainly affecting young and middle-aged women. Autoantibodies formation and immune complex deposition as well as other immune mechanisms contribute to heterogeneous clinical presentation, which leads to challenges for diagnosis and management. Although the exact pathogenesis of SLE is highly complicated, the pathophysiological understanding of SLE is constantly evolving and relevant studies were continually published, providing a better understanding of the molecular mechanisms. Moreover, new therapeutic strategies and management plans targeting pivotal factors involved in the pathogenesis of SLE got well established recently. In this article, we reviewed recent studies to provide an update in understanding pathogenesis and therapeutic strategies of SLE.
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http://dx.doi.org/10.1016/j.intimp.2020.107028DOI Listing
December 2020

A comparison and review of three sets of classification criteria for systemic lupus erythematosus for distinguishing systemic lupus erythematosus from pure mucocutaneous manifestations in the lupus disease spectrum.

Lupus 2020 Dec 7;29(14):1854-1865. Epub 2020 Oct 7.

Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University, Changsha, China.

Although the original purpose of the systemic lupus erythematosus (SLE) classification criteria was to distinguish SLE from other mimic diseases, and to facilitate sample selection in scientific research, they have become widely used as diagnostic criteria in clinical situations. It is not known yet if regarding classification criteria as diagnostic criteria, what problems might be encountered? This is the first study comparing the three sets of classification criteria for SLE, the 1997 American College of Rheumatology (ACR'97), 2012 Systemic Lupus International Collaborating Clinics (SLICC'12) and 2019 European League Against Rheumatism/American College of Rheumatology (EULAR/ACR'19), for their ability to distinguish patients with SLE from patients with pure mucocutaneous manifestations (isolated cutaneous lupus erythematosus without internal disease, i-CLE) in the lupus disease spectrum. 1,865 patients with SLE and 232 patients with i-CLE were recruited from a multicenter study. We found that, due to low specificity, none of the three criteria are adept at distinguishing patients with SLE from patients with i-CLE. SLICC'12 performed best among the original three criteria, but if a positive ANA was removed as an entry criterion, EULAR/ACR'19 would performed better. A review of previous studies that compared the three sets of criteria was presented in this work.
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http://dx.doi.org/10.1177/0961203320959716DOI Listing
December 2020

SARS-CoV-2 presented in the air of an intensive care unit (ICU).

Sustain Cities Soc 2021 Feb 15;65:102446. Epub 2020 Aug 15.

School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China.

As coronavirus disease 2019 (COVID-19) is spreading worldwide, there have been arguments regarding the aerosol transmission of its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Moreover, some re-detectable positive (RP) patients have been reported. However, little attention has been given to the follow-up of recovered patients, and there is no environmental evidence to determine whether these patients continue to shed the virus after they test negative. Therefore, with an objective to test the hypothesis of airborne transmission of SARS-CoV-2, it is necessary to 1) determine whether SARS-CoV-2 particles are present in the indoor air and 2) determine whether recovered patients are still shedding virus, thus providing much-needed environmental evidence for the management of COVID-19 patients during the recovery period. In this study, surface and air samples were collected from an intensive care unit (ICU) containing one ready-for-discharge patient. All surface samples tested negative, but the air samples tested positive for SARS-CoV-2. This implies that SARS-CoV-2 particles may be shed in aerosol form for days after patients test negative. This finding may be one of the reasons for the observation of RP patients; therefore, there is a need for improved clinical and disease management guidelines for recovered COVID-19 patients.
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http://dx.doi.org/10.1016/j.scs.2020.102446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7428766PMC
February 2021

Vitamin D status in patients with autoimmune bullous dermatoses: a meta-analysis.

J Dermatolog Treat 2020 Aug 26:1-12. Epub 2020 Aug 26.

Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: The association between autoimmune bullous dermatoses (AIBD) and serum vitamin D levels has been revealed by some studies, however, inconsistent.

Objectives: We aimed to evaluate the difference in vitamin D status between AIBD patients and controls.

Methods: We searched the studies about the vitamin D status of AIBD patients in electronic databases published before January 2020. Mean difference (MD) and 95% confidence intervals (CI) of eligible studies were calculated in meta-analyses of 25(OH)D levels. Pooled odds ratio (OR) and 95%CI were used in analyses of the prevalence of hypovitaminosis D. Different subgroup analyses, sensitivity analyses and publication bias assessment were conducted.

Results: We included nine case-control studies in the meta-analysis. Vitamin D level was significantly lower in both pemphigus (MD: -7.02, 95%CI: -10.30 to -3.74) and bullous pemphigoid (BP) (MD: -6.37, 95%CI: -12.15 to -0.58) patients than that in controls. Active pemphigus patients were at higher risk of presenting hypovitaminosis D (OR: 6.95, 95%CI: 1.37-35.25).

Conclusions: Abnormal vitamin D status are more common in AIBD patients than that in general population. Therefore, regular monitoring of vitamin D levels and vitamin D supplementation should be considered as part of the management strategy for AIBD.
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http://dx.doi.org/10.1080/09546634.2020.1810606DOI Listing
August 2020

A deep learning, image based approach for automated diagnosis for inflammatory skin diseases.

Ann Transl Med 2020 May;8(9):581

Department of Dermatology, Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China.

Background: As the booming of deep learning era, especially the advances in convolutional neural networks (CNNs), CNNs have been applied in medicine fields like radiology and pathology. However, the application of CNNs in dermatology, which is also based on images, is very limited. Inflammatory skin diseases, such as psoriasis (Pso), eczema (Ecz), and atopic dermatitis (AD), are very easily to be mis-diagnosed in practice.

Methods: Based on the EfficientNet-b4 CNN algorithm, we developed an artificial intelligence dermatology diagnosis assistant (AIDDA) for Pso, Ecz & AD and healthy skins (HC). The proposed CNN model was trained based on 4,740 clinical images, and the performance was evaluated on experts-confirmed clinical images grouped into 3 different dermatologist-labelled diagnosis classifications (HC, Pso, Ecz & AD).

Results: The overall diagnosis accuracy of AIDDA is 95.80%±0.09%, with the sensitivity of 94.40%±0.12% and specificity 97.20%±0.06%. AIDDA showed accuracy for Pso is 89.46%, with sensitivity of 91.4% and specificity of 95.48%, and accuracy for AD & Ecz 92.57%, with sensitivity of 94.56% and specificity of 94.41%.

Conclusions: AIDDA is thus already achieving an impact in the diagnosis of inflammatory skin diseases, highlighting how deep learning network tools can help advance clinical practice.
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http://dx.doi.org/10.21037/atm.2020.04.39DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290553PMC
May 2020

Retraction notice to "Increased abundance of nitrogen fixing bacteria by higher C/N ratio reduces the total losses of N and C in cattle manure and corn stover mix composting" [Waste Manage. 103 (2020) 416-425].

Waste Manag 2020 02 4;103:R1. Epub 2020 May 4.

Jilin Provincial Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology Chinese Academy of Sciences, Changchun 130102, PR China.

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http://dx.doi.org/10.1016/j.wasman.2020.04.034DOI Listing
February 2020

A Physics-Based DNI Model Assessing All-Sky Circumsolar Radiation.

iScience 2020 Mar 8;23(3):100893. Epub 2020 Feb 8.

Power Systems Engineering Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.

By investigating the long-term observations at Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP), we find that the routinely used Beer-Bouguer-Lambert law and the models that empirically separate direct normal irradiance (DNI) from measurements of global horizontal irradiance (GHI) have dramatic and unexpected bias in computing cloudy-sky DNI. This bias has led to tremendous uncertainty in estimating the electricity generation by solar energy conversion systems. To effectively reduce the bias, this study proposes a physical solution of all-sky DNI that computes solar radiation in the infinite-narrow beam along the sun direction and the scattered radiation falls within the circumsolar region. In sharp contrast with the other DNI models, this method uses a finite-surface integration algorithm that computes solar radiation in differential solid angles and efficiently infers its contribution to a surface perpendicular to the sun direction. The new model substantially reduces the uncertainty in DNI by a factor of 2-7.
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http://dx.doi.org/10.1016/j.isci.2020.100893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038471PMC
March 2020

Arg kinase mediates CXCL12/CXCR4-induced invadopodia formation and invasion of glioma cells.

Exp Cell Res 2020 04 5;389(1):111893. Epub 2020 Feb 5.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300052, PR China. Electronic address:

Compared with noninvasive tumor cells, glioma cells overexpress chemokine receptor type 4 (CXCR4), which exhibits significantly greater expression in invasive tumor cells than in noninvasive tumor cells. C-X-C motif chemokine ligand 12 (CXCL12, also known as stromal derived factor-1, SDF-1) and its cell surface receptor CXCR4 activate a signaling axis that induces the expression of membrane type-2 matrix metalloproteinase (MT2-MMP), which plays a pivotal role in the invasion and migration of various cancer cells; however, the specific mechanism involved in this is unclear. Recently, studies have shown that invadopodia can recruit and secrete related enzymes, such as matrix metalloproteinases (MMPs), to degrade the surrounding extracellular matrix (ECM), promoting the invasion and migration of tumor cells. Phosphorylated cortactin (pY421-cortactin) is required for the formation and maturation of invadopodia, but the upstream regulatory factors and kinases involved in phosphorylation have not been elucidated. In this study, we found that CXCL12/CXCR4 was capable of inducing glioma cell invadopodia formation, probably by regulating cortactin phosphorylation. The interaction of cortactin and Arg (also known as Abl-related nonreceptor tyrosine kinase, ABL2) in glioma cells was demonstrated. The silencing of Arg inhibited glioma cell invadopodia formation and invasion by blocking cortactin phosphorylation. Moreover, CXCL12 could not induce glioma cell invasion in Arg-knockdown glioma cells. Based on these results, it can be concluded that Arg mediates CXCL12/CXCR4-induced glioma cell invasion, and CXCL12/CXCR4 regulates invadopodia maturation through the Arg-cortactin pathway, which indicates that Arg could be a candidate therapeutic target to inhibit glioma cell invasion.
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http://dx.doi.org/10.1016/j.yexcr.2020.111893DOI Listing
April 2020

RETRACTED: Increased abundance of nitrogen fixing bacteria by higher C/N ratio reduces the total losses of N and C in cattle manure and corn stover mix composting.

Waste Manag 2020 02 14;103:416-425. Epub 2020 Jan 14.

Jilin Provincial Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology Chinese Academy of Sciences, Changchun 130102, PR China.

This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. The article duplicates significant parts of a paper that had already appeared in Bioresource Technology, Volume 297, February 2020, 122410, https://doi.org/10.1016/j.biortech.2019.122410. One of the conditions of submission of a paper for publication is that authors declare explicitly that the paper has not been previously published and is not under consideration for publication elsewhere. Re-use of any data should be appropriately cited. As such this article represents a misuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process.
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http://dx.doi.org/10.1016/j.wasman.2020.01.006DOI Listing
February 2020

Disordered cutaneous microbiota in systemic lupus erythematosus.

J Autoimmun 2020 03 26;108:102391. Epub 2019 Dec 26.

Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital of Central South University, Changsha, China; Research Unit of Key Technologies of Immune-related Skin Diseases Diagnosis and Treatment, Chinese Academy of Medical Sciences (2019RU027), Changsha, China. Electronic address:

The correlation between systemic lupus erythematosus (SLE) and microbiota colonization has been receiving much attention during recent years. Here, we screened the cutaneous bacterial spectrums of 69 SLE patients, 49 healthy controls and 20 dermatomyositis (DM) patients and identified the specific changes of cutaneous microbial composition and abundance in SLE patients. We observed the decreasing diversity in community richness and evenness and the greater heterogeneity in SLE patients compared to healthy controls, which were also different from the cutaneous microbiome of DM patients. The skin microbial community disorders in SLE patients were correlated with several clinical features such as serum low complement level, gender, renal involvement and myositis. According to the Kruskal-Wallis (KW) test, receiver operating characteristic (ROC) curve and LDA Effect Size (LEfSe) analysis, several bacterial taxa such as Staphylococcus, especially Staphylococcus aureus and Staphylococcus epidermidis, were identified to be potential markers for SLE skin lesions. Furthermore, Picrust analysis showed that Staphylococcus aureus infection pathway was significantly enriched and exhibited a strong correlation with genus Staphylococcus in SLE patients. The changes in the composition and abundance of cutaneous microbiota in SLE patients suggest that the microbial dysbiosis is associated with the pathogenesis of SLE, which may be potentially reliable biomarker or therapeutic target for SLE.
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http://dx.doi.org/10.1016/j.jaut.2019.102391DOI Listing
March 2020

Long-term combinations and updosing of second-generation H-antihistamines show efficacy and safety in the treatment of chronic spontaneous urticaria: A multicenter real-life pilot study.

J Allergy Clin Immunol Pract 2020 05 16;8(5):1733-1736.e11. Epub 2019 Dec 16.

Department of Dermatology, The First Hospital of China Medical University, National Health Commission Key Laboratory of Immunodermatology, Key Laboratory of Immunodermatology of Ministry of Education, Shenyang, China. Electronic address:

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http://dx.doi.org/10.1016/j.jaip.2019.12.006DOI Listing
May 2020

Identification of Key Pathways and Genes in the Orai2 Mediated Classical and Mesenchymal Subtype of Glioblastoma by Bioinformatic Analyses.

Dis Markers 2019 20;2019:7049294. Epub 2019 Oct 20.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China.

Background: Ca release-activated Ca channels (CRAC) are the main Ca entry pathway regulating intracellular Ca concentration in a variety of cancer types. Orai2 is the main pore-forming subunit of CRAC channels in central neurons. To explore the role of Orai2 in glioblastoma (GBM), we investigated the key pathways and genes in Orai2-mediated GBM by bioinformatic analyses.

Methods: Via The Cancer Genome Atlas (TCGA), French, Sun, and Gene Expression Omnibus (GEO) (GDS3885) datasets, we collected 1231 cases with RNA-seq data and analyzed the functional annotation of Orai2 by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Univariate and multivariate survival analyses were applied to 823 patients with survival data.

Results: We discovered that Orai2 was markedly upregulated in GBM compared to normal brain samples and lower-grade gliomas (LGG). Survival analysis found that higher expression of Orai2 was independently associated with a worse prognosis of patients with the classical and mesenchymal subtypes of GBM. Simultaneously, Orai2 expression was higher in tumors of the classical and mesenchymal subtypes than other subtypes and was significantly correlated with classical- and mesenchymal-related genes. GO and KEGG pathway analysis revealed that genes significantly correlated with Orai2 were involved in the JNK pathway. Through screening transcriptomic data, we found a strong association between Orai2 and apoptosis, stemness, and an epithelial-mesenchymal transition- (EMT-) like phenotype.

Conclusion: As a prognostic factor, Orai2 is obviously activated in the classical and mesenchymal subtypes of GBM and promotes glioma cell self-renewal, apoptosis, and EMT-like by the JNK pathway. These findings indicate that Orai2 could be a candidate prognostic and therapeutic target, especially for the classical and mesenchymal subtypes of GBM.
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http://dx.doi.org/10.1155/2019/7049294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6855003PMC
April 2020

Increased abundance of nitrogen transforming bacteria by higher C/N ratio reduces the total losses of N and C in chicken manure and corn stover mix composting.

Bioresour Technol 2020 Feb 13;297:122410. Epub 2019 Nov 13.

College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, PR China.

The aim of this work was to investigate how the initial C/N ratio during composting of chicken manure/corn stover mix affected the succession of dominant bacteria in the mix which led to the reduction of the total losses of N and C in the composting process. 16S rDNA sequencing indicated that the succession of predominant bacteria was significantly affected by the temperature and the initial C/N ratio during composting. Redundancy analysis showed that higher C/N appeared to promote the relative abundance of nitrogen fixing bacteria Thermoactinomyces, Planifilum, Flavobacterium, Bacillaceae, Pseudomonas,Sphingobacterium, Paenibacillus, Bacillus and Thermobifida, while compressing the denitrifying bacteria Pusillimonas, Ignatzschineria, Alcanivorax, Cerasibacillus, Truepera and Erysipelothrix. C/N ratio of 30:1 yielded the least C/N losses in the composting process, indicating that adjustment to the initial C/N ratio could affect nitrogen transforming bacteria to reduce the total losses of N and C and improve compost quality.
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http://dx.doi.org/10.1016/j.biortech.2019.122410DOI Listing
February 2020

Application of a "fish mouth flap" combined with an orbicularis oculi myocutaneous flap after surgical removal of basal cell carcinoma in the facial buccal region.

Indian J Dermatol Venereol Leprol 2019 Nov-Dec;85(6):649-652

Department of Dermatology, Medical Cosmetic Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

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http://dx.doi.org/10.4103/ijdvl.IJDVL_1003_18DOI Listing
March 2020

Correlation of CT texture changes with treatment response during radiation therapy for esophageal cancer: An exploratory study.

PLoS One 2019 26;14(9):e0223140. Epub 2019 Sep 26.

Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China.

Purpose: To analyze the change of CT texture features of esophageal squamous cell carcinoma (ESC) during RT delivery and to correlate these changes with the RT responses and survival.

Methods: A total of 61 ESC patients received radical RT were screened. Weekly CTs (4-6 sets for each patient) were acquired during RT. The tumors, normal esophageal mucosa tissue (NEC) of 5 cm and the spinal cord in the relevant area were delineated. CT texture features were extracted with a home-made tool. The changes of these features were analyzed by t-test. The correlations of the changes of features with RT responses and with patient survival were investigated by Pearson analysis.

Results: The average changes were increased by 0.00072 ±0.00197 for coarseness, by 0.14 ±0.40 for entropy, and by 2.34 ±3.56 for strength. In addition, the average changes were reduced by 8.88 ±15.71cc for volume and by 0.07 ±0.11 for busyness. The changes of the coarseness, strength, STD and entropy in ESC were different for the good and poor response groups. The survival rate of the patients was significantly correlated with the change of coarseness and strength (P = 0.0027 and P = 0.0001).

Conclusions: During RT, changes of CT texture features of ESC, e.g., coarseness, strength, STD, entropy and volume are correlated with radiation response and survival rate. With more clinical data and robust research, CT features, e.g., coarseness and strength, can be selected as outstanding imaging biomarkers for prediction of RT prognosis of ESC.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223140PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6762073PMC
April 2020

[Effects of slope direction on soil nutrient and its ecological stoichiometry in bamboo forest].

Ying Yong Sheng Tai Xue Bao 2019 Sep;30(9):2915-2922

Zhejiang Province Key Laboratory of Recycling and Eco-treatment of Waste Biomass, School of Environmental and Natural Resources, Zhejiang University of Science and Technology, Hangzhou 310023, China.

We analyzed the effects of slope direction on soil nutrients and ecological stoichiometry by collecting soil samples from different slope directions (shady slope and sunny slope) of the bamboo forest in Longyou County, Zhejiang Province. The results showed that soil nutrients were affected by slope direction and soil depth. The nutrients level of soils in the sampling area showed the trends of shady slope > sunny slope, and surface soil > bottom soil. Compared to sunny slope, the cation exchange capacity (CEC), the contents of total organic carbon, total nitrogen, alkaline hydrolyzed nitrogen, available phosphorus, total potassium and available potassium of shady soils significantly increased by 43.7%, 103.8%, 92.0%, 75.5%, 22.4%, 89.4% and 240.7%, respectively. There was no significant difference in total phosphorus contents between shady slope and sunny slope. At all soil layers, there was no significant difference of C/N ratio between shady and sunny slopes. The average C/P ratio of shady slope was 180.8%, 42.0% and 54.3% higher than that of sunny slope at 0-20 cm, 20-40 cm and 40-60 cm, respectively. At each soil layer, the average C/K and N/K ratios between shady and sunny slopes had no significant difference. The average C/K and N/K ratios of shady slope and sunny slope were all significantly different among the three soil layers. In the shady slope, the contents of soil organic carbon showed significantly positive correlation with total nitrogen, total phosphorus, total potassium, and soil available nutrients. Overall, soil nutrients and ecological stoichiometry characteristics of shady slope of bamboo forest were superior to those of sunny slope.
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http://dx.doi.org/10.13287/j.1001-9332.201909.025DOI Listing
September 2019

Notch1 signaling pathway promotes invasion, self-renewal and growth of glioma initiating cells via modulating chemokine system CXCL12/CXCR4.

J Exp Clin Cancer Res 2019 Aug 5;38(1):339. Epub 2019 Aug 5.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300052, China.

Background: Glioma initiating cells (GICs), also known as glioma stem cells (GSCs), play an important role in the progression and recurrence of glioblastoma multiforme (GBM) due to their potential for self-renewal, multiple differentiation and tumor initiation. In the recent years, Notch1 has been found to be overexpressed in GICs. However, the regulatory mechanism of Notch1 in the self-renewal and invasion ability of GICs remains unclear. This study aims to explore the effect of Notch pathway on self-renewal and invasion of GICs and the underlying mechanisms.

Methods: Bioinformatic analysis and immunohistochemistry (IHC) were performed to evaluate the expression of Notch1 and Hes1 in GBM samples. Immunofluorescent (IF) staining was performed to observe the distribution of Notch1 and CXCR4 in GBM and GICs. Both pharmacological intervention and RNA interference were employed to investigate the role of Notch1 in GICs self-renewal, invasion and tumor growth in vitro or in vivo. The crosstalk effect of Notch1 and CXCL12/CXCR4 system on GIC self-renewal and invasion was explored by sphere formation assay, limiting dilution assay and Transwell assay. Western blots were used to verify the activation of Notch1/CXCR4/AKT pathway in self-renewal, invasion and tumor growth of GICs. Luciferase reporter assay was used to testify the potential binding site of Notch1 signaling and CXCR4. The orthotopic GICs implantations were established to analyze the role and the mechanism of Notch1 in glioma progression in vivo.

Results: Notch1 signaling activity was elevated in GBM tissues. Notch1 and CXCR4 were both upregulated in GICs, compared to Notch1 positive glioma cells comprised a large proportion in the CD133+ glioma cell spheres, CXCR4 positive glioma cells which usually expressed Notch1 both and dispersed in the periphery of the sphere, only represent a small subset of CD133+ glioma cell spheres. Furthermore, downregulation of the Notch1 pathway by shRNA and MK0752 significantly inhibited the PI3K/AKT/mTOR signaling pathway via the decreased expression of CXCR4 in GICs, and weakened the self-renewal, invasion and tumor growth ability of GICs.

Conclusions: These findings suggest that the cross-talk between Notch1 signaling and CXCL12/CXCR4 system could contribute to the self-renewal and invasion of GICs, and this discovery could help drive the design of more effective therapies in Notch1-targeted treatment of GBMs.
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http://dx.doi.org/10.1186/s13046-019-1319-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683584PMC
August 2019