Publications by authors named "Hai Lin"

612 Publications

The involvement of FATP1 regulating skeletal muscle fat deposition in stressed broilers was affected by fatty acid substrates.

Front Vet Sci 2022 15;9:965894. Epub 2022 Jul 15.

Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science & Technology, Shandong Agricultural University, Taian, China.

Fatty acid transport protein 1 (FATP1), plays a major role in the transport and uptake of fatty acids into cells. The effect of FATP1 on the regulation of skeletal muscle fat uptake and deposition in stressed broiler chickens was investigated both and , and the effect of different fatty acid substrates were also included. Dexamethasone (DEX), a synthetic glucocorticoid (GCs), was employed to induce a hyper glucocorticoid milieu and simulate stress. The results showed that DEX would increase the mRNA expression of FATP1 and fat deposition in muscle tissues ( < 0.05), the very-low-density lipoprotein (VLDL) and insulin (INS) levels were significantly increased in the plasma by DEX ( < 0.05), and the mRNA levels of the glucocorticoid receptor (), adiponectin receptor () and peroxisomal proliferator-activated receptor α (α) in thigh were also up-regulated by DEX ( < 0.05). experiment, DEX did not affect the myoblast fat deposition and α and expressions without the external fatty acid ( > 0.05). Under PA pre-treatment, both myoblast fatty acid uptake and fat deposition were promoted by DEX treatment ( < 0.05), and the effects of DEX on the gene expressions of α and were upregulated first and then downregulated as the dose of DEX increases; while under OA pre-treatment, the myoblast fat deposition was not affected by DEX ( > 0.05), the fatty acid uptake was decreased by DEX at 500 nM compared to control ( < 0.05). When GR and PPARα were, respectively inhibited by specific inhibitors RU486 and GW6471, the effects of DEX on fatty acid uptake were reversed for PA pre-treated myoblasts ( < 0.05) but not for OA pre-treated myoblasts ( > 0.05). These results indicate that FATP1 regulation by GCs was affected by fatty acid substrate - saturated fatty acids were favorable for fat uptake and deposition, while unsaturated fatty acids were not. GCs may affect the ADPNR-PPARα-FATP1 pathway by binding to its receptors, thus regulating the uptake of saturated fatty acids into myoblasts.
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http://dx.doi.org/10.3389/fvets.2022.965894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334852PMC
July 2022

High ambient humidity aggravates ammonia-induced respiratory mucosal inflammation by eliciting Th1/Th2 imbalance and NF-κB pathway activation in laying hens.

Poult Sci 2022 Jun 23;101(9):102028. Epub 2022 Jun 23.

Department of Animal Science and Technology, Shandong Agricultural University, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an City, Shandong Province 271018, China. Electronic address:

Ammonia (NH) is an irritant and harmful gas. Its accumulation in the poultry house poses detrimental effects on the respiratory mucosal system of birds. In this process, the relative humidity of the poultry house also plays an important role in potentiating the adverse effects of NH on the respiratory status of birds, causing severe physiological consequences. In this study, the combined effects of NH and humidity on the respiratory mucosal barrier of laying hens was studied. The gene expression of tight junction proteins, mucin, inflammatory cytokines secreted by Th1/Th2 cells, and proteins related to the Nuclear factor-κB (NF-κB) signaling pathway were detected by qRT-PCR. In addition, the contents of mucin and secretory immunoglobulin A (SIgA) in bronchoalveolar lavage fluid (BALF) were determined. The results showed that treatment with NH alone or NH and humidity led to morphological changes in the respiratory tract, decreased the gene expressions of tight junction protein, and increased the expression of mucin. Also, the expression of interleukin-4 (IL-4) and IL-10 were increased, whereas, the expression of interferon-γ (IFN-γ) and IL-2 was decreased in laying hens treated with NH and humidity. Furthermore, the activation of inhibitor kappa B kinase β (I-KK-β) and the degradation of inhibitor of NF-κB α (I-κB-α) contributed to the activation of the NF-κB pathway, such that the downstream genes, cycooxygenase 2 (COX2) and inducible nitric oxide synthase (iNOS) were significantly increased. In conclusion, NH damaged the mucosal barrier and induced an imbalance in the mucosal immunity, leading to respiratory tract inflammation. Thus, the relative humidity of the environment aggravates the adverse effects of NH in poultry.
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http://dx.doi.org/10.1016/j.psj.2022.102028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326132PMC
June 2022

C1QTNF6 regulated by miR-29a-3p promotes proliferation and migration in stage I lung adenocarcinoma.

BMC Pulm Med 2022 Jul 25;22(1):285. Epub 2022 Jul 25.

Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian Province, China.

Objective: C1QTNF6 has been implicated as an essential component in multiple cellular and molecular preliminary event, including inflammation, glucose metabolism, endothelial cell modulation and carcinogenesis. However, the biological process and potential mechanism of C1QTNF6 in lung adenocarcinoma (LUAD) are indefinite and remain to be elucidated. Therefore, we investigated the interaction among the traits of C1QTNF6 and LUAD pathologic process.

Methods: RT-qPCR and western blot were conducted to determine the expression levels of C1QTNF6. RNA interference and overexpression of C1QTNF6 were constructed to identify the biological function of C1QTNF6 in cellular proliferative, migratory and invasive potentials in vitro. Dual-luciferase reporter assay was applied to identify the possible interaction between C1QTNF6 and miR-29a-3p. Moreover, RNA sequencing analysis of C1QTNF6 knockdown was performed to identify the potential regulatory pathways.

Results: C1QTNF6 was upregulated in stage I LUAD tissues compared with adjacent non-cancerous tissues. Concurrently, C1QTNF6 knockdown could remarkably inhibit cell proliferation, migratory and invasive abilities, while overexpression of C1QTNF6 presented opposite results. Additionally, miR-29a-3p may serve as an upstream regulator of C1QTNF6 and reduce the expression of C1QTNF6. Subsequent experiments showed that miR-29a-3p could decrease the cell mobility and proliferation positive cell rates, as well as reduce the migratory and invasive possibilities in LUAD cells via downregulating C1QTNF6. Moreover, RNA sequencing analysis demonstrated that the cytokine-cytokine receptor interaction pathway may participate in the process of C1QTNF6 regulating tumor progression.

Conclusion: Our study first demonstrated that downregulation of C1QTNF6 could inhibit tumorigenesis and progression in LUAD cells negatively regulated by miR-29a-3p. These consequences could reinforce our awareness and understanding of the underlying mechanism and provide a promising therapeutic target for LUAD.
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http://dx.doi.org/10.1186/s12890-022-02055-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310408PMC
July 2022

CD40×HER2 bispecific antibody overcomes the CCL2-induced trastuzumab resistance in HER2-positive gastric cancer.

J Immunother Cancer 2022 Jul;10(7)

Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China

Background: There was much hard work to study the trastuzumab resistance in HER2-positive gastric cancer (GC), but the information which would reveal this abstruse mechanism is little. In this study, we aimed to investigate the roles of tumor cell-derived CCL2 on trastuzumab resistance and overcome the resistance by treatment with the anti-CD40-scFv-linked anti-HER2 (CD40 ×HER2) bispecific antibody (bsAb).

Methods: We measured the levels of CCL2 expression in HER2-positive GC tissues, and revealed biological functions of tumor cell-derived CCL2 on tumor-associated macrophages (TAMs) and the trastuzumab resistance. Then, we developed CD40 ×HER2 bsAb, and examined the targeting roles on HER2 and CD40, to overcome the trastuzumab resistance without systemic toxicity.

Results: We found the level of CCL2 expression in HER2-postive GC was correlated with infiltration of TAMs, polarization status of infiltrated TAMs, trastuzumab resistance and survival outcomes of GC patients. On exposure to CCL2, TAMs decreased the M1-like phenotype, thereby eliciting the trastuzumab resistance. CCL2 activated the transcription of ZC3H12A, which increased K63-linked deubiquitination and K48-linked auto-ubiquitination of TRAF6/3 to inactivate NF-κB signaling in TAMs. CD40 ×HER2 bsAb, which targeted the CD40 to restore the ubiquitination level of TRAF6/3, increased the M1-like phenotypic transformation of TAMs, and overcame trastuzumab resistance without immune-related adversary effects (irAEs).

Conclusions: We revealed a novel mechanism of trastuzumab resistance in HER2-positive GC via the CCL2-ZC3H12A-TRAF6/3 signaling axis, and presented a CD40 ×HER2 bsAb which showed great antitumor efficacy with few irAEs.
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http://dx.doi.org/10.1136/jitc-2022-005063DOI Listing
July 2022

Highly promoted phytoremediation with endophyte inoculation in multi-contaminated soil: plant biochemical and rhizosphere soil ecological functioning behavior.

Environ Sci Pollut Res Int 2022 Jul 18. Epub 2022 Jul 18.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

Rhizosphere soil ecological functioning behavior is of critical importance for regulating phytoremediation efficiency during microbial-assisted phytoremediation for multi-heavy metal-polluted soils. In this study, Trifolium repens L. and its endophyte Pseudomonas putida were used to investigate the ecological responses of the microbe-plant-soil system in Cd, Cr, and Pb co-contaminated soil. The results showed that endophyte Pseudomonas putida significantly increased plant biomass by 22.26-22.78% and phytoremediation efficiency by 29.73-64.01%. The increased phytoremediation efficiency may be related to the improvement of photosynthetic pigment content and antioxidant enzyme activities in leaves and the enhancement of rhizosphere soil ecological functioning. With endophyte application, soil nutrient content was significantly increased and heavy metal bioavailability was enhanced that residual fraction was reduced by 3.79-12.87%. Besides, the relative abundance of ecologically beneficial rhizobacteria such as Bacteriovorax and Arthrobacter was increased by 3.04-8.53% and 0.80-1.64%, respectively. Endophyte inoculation also significantly increased all the functional genes involved in cellular processes, genetic information processing, environmental information processing, and metabolism. This study indicated that the application of endophytes has a positive effect on the biochemical responses of Trifolium repens L. and could significantly improve rhizosphere ecological functioning in multi-heavy metal contamination, which provided clear strategies for regulating phytoremediation.
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http://dx.doi.org/10.1007/s11356-022-21689-7DOI Listing
July 2022

A super pan-genomic landscape of rice.

Cell Res 2022 Jul 12. Epub 2022 Jul 12.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang, China.

Pan-genomes from large natural populations can capture genetic diversity and reveal genomic complexity. Using de novo long-read assembly, we generated a graph-based super pan-genome of rice consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice. Our pan-genome reveals extensive structural variations (SVs) and gene presence/absence variations. Additionally, our pan-genome enables the accurate identification of nucleotide-binding leucine-rich repeat genes and characterization of their inter- and intraspecific diversity. Moreover, we uncovered grain weight-associated SVs which specify traits by affecting the expression of their nearby genes. We characterized genetic variants associated with submergence tolerance, seed shattering and plant architecture and found independent selection for a common set of genes that drove adaptation and domestication in Asian and African rice. This super pan-genome facilitates pinpointing of lineage-specific haplotypes for trait-associated genes and provides insights into the evolutionary events that have shaped the genomic architecture of various rice species.
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http://dx.doi.org/10.1038/s41422-022-00685-zDOI Listing
July 2022

voxel2vec: A Natural Language Processing Approach to Learning Distributed Representations for Scientific Data.

IEEE Trans Vis Comput Graph 2022 Jul 7;PP. Epub 2022 Jul 7.

Relationships in scientific data, such as the numerical and spatial distribution relations of features in univariate data, the scalar-value combinations' relations in multivariate data, and the association of volumes in time-varying and ensemble data, are intricate and complex. This paper presents voxel2vec, a novel unsupervised representation learning model, which is used to learn distributed representations of scalar values/scalar-value combinations in a low-dimensional vector space. Its basic assumption is that if two scalar values/scalar-value combinations have similar contexts, they usually have high similarity in terms of features. By representing scalar values/scalar-value combinations as symbols, voxel2vec learns the similarity between them in the context of spatial distribution and then allows us to explore the overall association between volumes by transfer prediction. We demonstrate the usefulness and effectiveness of voxel2vec by comparing it with the isosurface similarity map of univariate data and applying the learned distributed representations to feature classification for multivariate data and to association analysis for time-varying and ensemble data.
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http://dx.doi.org/10.1109/TVCG.2022.3189094DOI Listing
July 2022

Technologies for removing heavy metal from contaminated soils on farmland: A review.

Chemosphere 2022 Oct 23;305:135457. Epub 2022 Jun 23.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, Beijing, 100083, China. Electronic address:

With the rapid development of industrialization and agricultural intensification and scale, the problem of heavy metal pollution in farmland has attracted widespread attention. There exist various methods to remediate heavy metal contaminated soils, but the sources and extent of contamination in agricultural soils are more complex and require higher selection of remediation technologies. This paper discusses in detail the status of heavy metal pollution in agricultural fields and the research progress of removal technologies and provides an in-depth analysis and comparison of remediation technologies from three aspects: physicochemical, electrochemical, and biological, which provides references and directions for the selection of future remediation technologies in agricultural fields. It was found that the existing morphology of soil heavy metals is an important reference for the selection of soil remediation methods; in practical applications, both phytoremediation and removal of heavy metals from agricultural soils using magnetic biochar have good potential for development. Phytoremediation of farmland through crop rotation or intercropping can not only achieve remediation while producing, but also optimize the soil environment. However, phytoremediation requires a high degree of pollution in the soil environment, the selection of plant species is a key step in phytoremediation and improving the tolerance of plants to heavy metals requires in-depth research. Magnetic biochar is an ideal material for soil remediation because of its relatively short remediation time and reusable materials and metals. However, the binding mechanism between magnetic biochar and heavy metals, the effect of magnetic biochar on soil microorganisms to be further studied.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135457DOI Listing
October 2022

The fibroblast growth factor receptor antagonist SSR128129E inhibits fat accumulation via suppressing adipogenesis in mice.

Mol Biol Rep 2022 Jun 22. Epub 2022 Jun 22.

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.

Background: AS an allosteric inhibitor of fibroblast growth factor receptors (FGFRs), SSR128129E (SSR) extensively inhibits the fibroblast growth factor (FGF) signaling. Given the metabolic importance of FGFs and the global epidemic of obesity, we explored the effect of SSR on fat metabolism.

Methods And Results: Three-week-old male mice were administered intragastrically with SSR (30 mg/kg/day) or PBS for 5 weeks. The effects of SSR on white and brown fat metabolism were investigated by respiratory metabolic monitoring, histological assessment and molecular analysis. Results indicated that SSR administration significantly reduced the body weight gain and the fat content of mice. SSR did not increase, but decreased the thermogenic capability of both brown and white fat. However, SSR markedly suppressed adipogenesis of adipose tissues. Further study demonstrated the involvement of ERK signaling in the action of SSR.

Conclusions: SSR may be a promising drug candidate for the prevention of obesity via suppressing adipogenesis. However, the influence of SSR on thermogenesis in humans should be further investigated before its clinical application.
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http://dx.doi.org/10.1007/s11033-022-07699-1DOI Listing
June 2022

How heavy metal stress promotes dissemination of antibiotic resistance genes in the activated sludge process.

J Hazard Mater 2022 09 2;437:129279. Epub 2022 Jun 2.

School of Environment, Tsinghua University, Beijing 100084, China. Electronic address:

Heavy metals have been recently revealed as promoters to antibiotic resistance gene (ARG) dissemination in water environment, but their influence on ARG transfer in the activated sludge process has not been clear. In this study, a set of sequencing batch reactors (SBRs) and micro-scale microfluidic chips were established to quantify the impacts of heavy metals (0.5 mM of Pb, 0.1 mM of As, and 0.005 mM of Hg) on the ARG spreading in the activated sludge consortium. Under heavy metal stress, transfer frequencies were 1.7-3.6 folds increase compared to the control. Gram-negative bacteria increased significantly after heavy metal added, which were more prone to receiving resistant plasmid from donors. Meanwhile, the relative expression of genes related to conjugation changed in activated sludge, especially the expression of outer membrane protein and oxidative stress regulatory genes increased by 2.9-7.4 folds and 7.8-13.1 folds, respectively. Furthermore, using microfluidic chips, the dynamics of ARG transfer was observed at single cell level under heavy metal pressure. Heavy metals firstly promoted conjugation and then vertical gene transfer played an important part for ARG spreading. The results provided in-depth understanding of the influence of heavy metals on ARG behavior in the environment.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129279DOI Listing
September 2022

The optimal dietary arginine level of laying hens fed with low-protein diets.

J Anim Sci Biotechnol 2022 Jun 17;13(1):63. Epub 2022 Jun 17.

Department of Animal Science, Shandong Agricultural University, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, 61 Daizong Street, Taian City, 271018, Shandong Province, China.

Background: Arginine (Arg) is an essential amino acid (EAA) in poultry, an important substrate for protein synthesis and a precursor of several molecules. Supplementation of EAAs with low protein (LP) diet increases the utilization efficiency of dietary crude protein (CP). However, if the EAA requirement is changed in hens fed a LP diet remains to be elucidated. The aim of the present study was to evaluate the optimal level of dietary Arg in the LP diet of hens. A total of 1350 Hy-Line Brown laying hens were randomly allocated to six dietary treatments: a basal diet (16% CP, positive control), or an isoenergetic LP diet (14% CP, 0.80% Arg) supplemented 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg, corresponding to 0.80%, 0.85%, 0.90%, 0.95% and 1.00% dietary Arg, respectively.

Results: The feed efficiency was decreased (P < 0.05) by 0.80% and 1.00% Arg-LP diets, compared to control. Within LP diets, dietary Arg level had significant quadratic effects (P < 0.05) on laying rate, egg mass, and feed efficiency. Compared to control, the plasma CAT activity or T-AOC content were decreased by 0.80% (P < 0.001). However, the hens offered 0.85% and 0.90% Arg-LP diets had higher CAT activity (P < 0.001) than 0.80% Arg-LP diet. In contrast, 1.00% Arg-LP group had the highest MDA and the lowest T-AOC content in plasma, liver, duodenal and jejunal mucosa (P < 0.05). Compared to control, the villus height was decreased by 0.80%, 0.95% and 1.00% Arg-LP diets, while the villus height to crypt depth (V/C) ratio was reduced by 0.95% and 1.00% Arg-LP diets in duodenum.

Conclusion: The result demonstrates that LP diet (14% CP) deficient in Arg (0.80% Arg) result in augmented oxidative damage and impaired development of intestinal mucosa. According to the quadratic broken-line regression model, the optimal dietary arginine levels for Hy-Line Brown laying hens fed with low protein diet (14% CP) aged 33 to 40 weeks are 0.85%, 0.86%, and 0.86% to obtained the maximum laying rate, egg mass, and feed efficiency, respectively.
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http://dx.doi.org/10.1186/s40104-022-00719-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206374PMC
June 2022

Construction and carbon source optimization of a microbial-plant coupled reactor for treating acid mine drainage.

Environ Sci Pollut Res Int 2022 Jun 14. Epub 2022 Jun 14.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

Acid mine drainage (AMD) is recognized as one of the most serious contamination sources in the nonferrous metal mining industry. In this study, aerobic strains VCZ02 and VCZ09, which were identified as Leclercia adecarboxylata and Klebsiella aerogenes, were screened from 11 strains of copper-zinc-resistant bacteria in the soil of the Dexing copper mine with Cu/Zn removal rates of 46.32%/41.03% and 57.96%/67.05%, respectively. The composition of extracellular polymers plays an important role in the removal of heavy metals by these two strains. A mixed community consisting of VCZ02 and VCZ09 was coupled with Sagittaria trifolia L.var.sinensis (Sims) Mak to construct a microbial-plant coupled reactor to remediate AMD. Under the optimal condition of sodium acetate as carbon source, the pH of AMD increased from less than 5 to above 6.5, showing Cu/Zn removal rates of 70-80% and above 30%, respectively. SEM-EDS results showed that VZC02 and VZC09 in the coupled reactor also helped with resisting the toxicity of heavy metals to plants by forming biofilms on the root surface and increasing the content of heavy metals on the surface of roots, thus improving the treatment effect of plants. This study provides a theoretical basis for the bioremediation of AMD and its application.
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http://dx.doi.org/10.1007/s11356-022-21329-0DOI Listing
June 2022

Simultaneous removal of high concentrations of ammonia nitrogen and calcium by the novel strain Paracoccus denitrificans AC-3 with good environmental adaptability.

Bioresour Technol 2022 Sep 11;359:127457. Epub 2022 Jun 11.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China. Electronic address:

The novel Paracoccus denitrificans AC-3 strain was isolated and displayed outstanding purification capability for high concentrations of ammonia nitrogen (NH-N) and calcium (Ca). Meanwhile, the strain exhibited excellent environmental adaptability within a wide pH range and high levels of NH-N and Ca. Nitrogen balance analysis demonstrated that the pathways of NH-N removal consisted of 80.12% assimilation and 16.60% heterotrophic nitrification aerobic denitrification (HNAD). In addition, Ca was removed by forming calcium carbonate (CaCO) with carbonate (CO) and bicarbonate (HCO). COand HCO were obtained from carbon dioxide (CO) hydration, which was catalyzed by carbonic anhydrase (CA) secreted by strain AC-3. The alkaline environment for carbonate precipitation was provided by CA and HNAD. The resulting CaCO existed in the form of calcite and exhibited a unique morphology and elemental composition.
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http://dx.doi.org/10.1016/j.biortech.2022.127457DOI Listing
September 2022

Construction of bifunctional bacterial community for co-contamination remediation: Pyrene biodegradation and cadmium biomineralization.

Chemosphere 2022 Oct 11;304:135319. Epub 2022 Jun 11.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, Beijing, 100083, China.

Polycyclic aromatic hydrocarbons and heavy metals are typical pollutants in the non-ferrous metal smelting industry. The combination of biodegradation and biomineralization has great development potential for co-contamination removal as an environmentally friendly method. Pyrene (Pyr) and cadmium (Cd) were regarded as model pollutants of co-contamination in this study. A bifunctional bacterial community named Ycp was screened from a non-ferrous smelting slag field soil. The 16S rRNA gene high throughput sequencing analysis showed that Enterobacter was the dominant genus (99.1%). Ycp had adaptability under a wide range of environmental conditions (pH 3-9, salinity 0-10 g L NaCl, Pyr concentration 0-50 mg L, Cd concentration 0-100 mg L), and the removal rate of Pyr and Cd reached 41.8%-76.9%, 82.8%-98.8%, respectively. It was found that compound carbon sources had promoting effect on the removal of Pyr and Cd, with the maximum removal rate of 88.3% and 98.0%. According to the degradation products of Pyr by LC-MS analysis and the mineralized products of Cd by XRD and SEM-EDS analysis, the mechanism of Ycp for co-contamination remediation was: Ycp biodegraded Pyr through salicylic acid and phthalic acid metabolic pathways, and biomineralized Cd into CdCO through microbially induced carbonate precipitation. This study provided a basis for microbial remediation of co-contamination.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135319DOI Listing
October 2022

The behavior of heavy metal release from sulfide waste rock under microbial action and different environmental factors.

Environ Sci Pollut Res Int 2022 Jun 2. Epub 2022 Jun 2.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

The dissolution of heavy metals from the waste rock is controlled by many factors. Herein, we investigated the release behavior of iron (Fe), chromium (Cr), copper (Cu), and zinc (Zn) from sulfide waste rock under the actions of microorganisms and different environmental factors (solution pH value, particle size of waste rock, temperature, Fe concentration). The release quantity of heavy metals was negatively correlated with pH and particle size and positively correlated with ambient temperature and Fe concentration. Under the experimental conditions of pH value of 3.0, temperature of 35°C, and waste stone particle size of less than 0.075 mm,, the release quantity of Fe, Cr, Cu, and Zn reached 3680, 18.32, 132.20, 26.60 mg·kg after 20 days of leaching, respectively. Rising the temperature to 45 °C, Fe, Cr, Cu, and Zn release quantities increased to 89.30, 5.81, 105.08, and 28.00 mg·kg. Six hundred milligrams per liter Fe increased the release of heavy metals considerably (2.63-65.48 folds). The presence of microorganisms can significantly facilitate the release of heavy metals. Compared to the control group, the release quantities of Fe, Cr, Cu, and Zn increased 4.29, 3.17, 1.54, and 2.39 times, respectively. In addition, the waste rock under microbial action was more seriously corroded than that under chemical factors. The release behavior of these four heavy metals was consistent with the interfacial chemical reaction control model, indicating that the reactions mainly occurred on the surface of the waste rock. This study provides an essential reference for the study of heavy metal leaching behavior.
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http://dx.doi.org/10.1007/s11356-022-20555-wDOI Listing
June 2022

L-Arginine/nitric oxide regulates skeletal muscle development via muscle fibre-specific nitric oxide/mTOR pathway in chickens.

Anim Nutr 2022 Sep 2;10:68-85. Epub 2022 May 2.

Key Lab for Animal Biotechnology and Disease Control, Department of Animal Science, Shandong Agricultural University, Tai'an, 271000, China.

L-Arginine (L-Arg), the precursor of nitric oxide (NO), plays an important role in muscle function. Fast-twitch glycolytic fibres are more susceptible to age-related atrophy than slow-twitch oxidative fibres. The effect of L-Arg/NO on protein metabolism of fast- and slow-twitch muscle fibres was evaluated in chickens. In Exp. 1, 48 chicks at 1 day old were divided into 4 groups of 12 birds and subjected to 4 treatments: basal diet without supplementation or supplemented with 1% L-Arg, and water supplemented with or without L-nitro-arginine methyl ester (L-NAME, 18.5 mM). In Exp. 2, 48 chicks were divided into 4 groups of 12 birds fed with the basal diet and subjected to the following treatments: tap water (control), tap water supplemented with L-NAME (18.5 mM), or molsidomine (MS, 0.1 mM), or 18.5 mM L-NAME + 0.1 mM MS (NAMS). The regulatory effect of L-Arg/NO was further investigated in vitro with myoblasts obtained from chicken embryo pectoralis major (PM) and biceps femoris (BF). In vivo, dietary L-Arg supplementation increased breast (+14.94%,  < 0.05) and thigh muscle mass (+23.40%,  < 0.05); whereas, MS treatment had no detectable influence. However, L-NAME treatment blocked the beneficial influence of L-Arg on muscle development. L-Arg decreased ( < 0.05) protein synthesis rate, phosphorylated mTOR and ribosomal protein S6 kinase beta-1 (p70S6K) levels in breast muscle, which was recovered by L-NAME treatment. In vitro, L-Arg or sodium nitroprusside (SNP) reduced protein synthesis rate, suppressed phosphorylated mTOR/p70S6K and decreased atrogin-1 and muscle RING finger 1 (MuRF1) in myoblasts from PM muscle ( < 0.05). L-NAME abolished the inhibitory effect of L-Arg on protein synthesis and the mTOR/p70S6K pathway. However, myoblasts from BF muscle showed the weak influence. Moreover, blocking the mTOR/p70S6K pathway with rapamycin suppressed protein synthesis of the 2 types of myoblasts; whereas, the protein expression of atrogin-1 and MuRF1 levels were restricted only in myoblasts from PM muscle. In conclusion, L-Arg/NO/mTOR/p70S6K pathway enhances protein accumulation and muscle development in fast-twitch glycolytic muscle in chickens. L-Arg/NO regulates protein turnover in a muscle fibre specific way, which highlights the potential clinical application in fast-twitch glycolytic muscle fibres.
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http://dx.doi.org/10.1016/j.aninu.2022.04.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125674PMC
September 2022

Advances in Regenerative Sports Medicine Research.

Front Bioeng Biotechnol 2022 13;10:908751. Epub 2022 May 13.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Regenerative sports medicine aims to address sports and aging-related conditions in the locomotor system using techniques that induce tissue regeneration. It also involves the treatment of meniscus and ligament injuries in the knee, Achilles' tendon ruptures, rotator cuff tears, and cartilage and bone defects in various joints, as well as the regeneration of tendon-bone and cartilage-bone interfaces. There has been considerable progress in this field in recent years, resulting in promising steps toward the development of improved treatments as well as the identification of conundrums that require further targeted research. In this review the regeneration techniques currently considered optimal for each area of regenerative sports medicine have been reviewed and the time required for feasible clinical translation has been assessed. This review also provides insights into the direction of future efforts to minimize the gap between basic research and clinical applications.
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http://dx.doi.org/10.3389/fbioe.2022.908751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136559PMC
May 2022

Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface.

Bioact Mater 2023 Feb 17;20:1-15. Epub 2022 May 17.

National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, Sichuan, China.

Healing of an anterior cruciate ligament (ACL) autologous graft in a bone tunnel occurs through the formation of fibrovascular scar tissue, which is structurally and compositionally inferior to normal fibrocartilaginous insertion and thus may increase the reconstruction failure and the rate of failure recurrence. In this study, an injectable hydroxyapatite/type I collagen (HAp/Col Ⅰ) paste was developed to construct a suitable local microenvironment to accelerate the healing of bone-tendon interface. Physicochemical characterization demonstrated that the HAp/Col Ⅰ paste was injectable, uniform and stable. The cell culture illustrated that the paste could promote MC3T3-E1 cells proliferation and osteogenic expression. The results of a canine ACL reconstruction study showed that the reconstructive ACL had similar texture and color as the native ACL. The average width of the tunnel, total bone volume, bone volume/tissue volume and trabecular number acquired from micro-CT analysis suggested that the healing of tendon-bone interface in experimental group was better than that in control group. The biomechanical test showed the maximal loads in experimental group achieved approximately half of native ACL's maximal load at 24 weeks. According to histological examination, Sharpey fibers could be observed as early as 12 weeks postoperatively while a typical four-layer transitional structure of insertion site was regenerated at 48 weeks in the experimental group. The injectable HAp/Col Ⅰ paste provided a biomimetic scaffold and microenvironment for early cell attachment and proliferation, further osteogenic expression and extracellular matrix deposition, and structural and functional regeneration of the tendon-bone interface.
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http://dx.doi.org/10.1016/j.bioactmat.2022.05.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123091PMC
February 2023

Evidence-based biomaterials research.

Bioact Mater 2022 Sep 25;15:495-503. Epub 2022 Apr 25.

Institute of Regulatory Science for Medical Devices, Sichuan University, Chengdu, 610064, PR China.

The fast development of biomaterials science and engineering has generated significant number of studies and publications as well as tremendous amount of research data. A methodology is needed to translate such research data and results to validated scientific evidence. This article for the first time proposes the concept and methodology of evidence-based biomaterials research, which is to use evidence-based research approach represented by systematic reviews to generate evidence for answering scientific questions related to biomaterials. After briefly introducing the advancement of biomaterials since 1950s, the scientific and engineering nature of biomaterials are discussed along with the roadmap of biomaterials translation from basic research to commercialized medical products, and the needs of scientific evidence. Key information of the evidence-based approach such as its origination from evidence-based medicine, levels of evidence, systematic review and meta-analysis, differences between systematic and narrative reviews is then highlighted. Applications with a step-by-step procedure of conducting evidence-based biomaterials research, three examples of biomaterials research using evidence-based approach to generate scientific evidence, and opportunities and challenges of evidence-based biomaterials research are presented. With its notable impact on the practice of medicine, the evidence-based approach is also expected to make influential contributions to the biomaterials field.
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http://dx.doi.org/10.1016/j.bioactmat.2022.04.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122837PMC
September 2022

Investigation of the relationship between microbiota dynamics and volatile changes in chilled beef steaks held under high-oxygen packaging enriched in carbon dioxide.

Meat Sci 2022 Sep 20;191:108861. Epub 2022 May 20.

Lab of Beef Processing and Quality Control, College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; National R&D Center for Beef Processing Technology, Tai'an, Shandong 271018, PR China. Electronic address:

Carbon dioxide (CO) plays an important bacteriostatic role in high-oxygen modified atmosphere packaging (HiOx-MAP). This study aimed to explore the influence of CO on the volatile organic compounds (VOCs) profile in HiOx-MAP beef steaks and assess the changes in VOCs associated with microbiota dynamics during 20-days of chilled storage. Compared to control MAP (CMAP: 50% O/50% N), CO-enriched MAP (TMAP: 50% O/40% CO/10% N) rendered steaks with desirable appearance and odor freshness during storage through inhibiting bacterial growth and reducing the accumulation of acetoin, 2-ethyl-1-hexanol, 1,3-butanediol, diacetyl and 2-heptanone (P < 0.05). 1-Octen-3-ol and 2-ethyl-1-hexanol were main VOCs in TMAP steaks and were positively related to Brochothrix thermosphacta levels. Acetoin and 2-ethyl-1-hexanol could be used as potential spoilage indicators in CMAP steaks, which were positively related to B. thermosphacta and Pseudomonas levels. Overall, the desirable odor freshness of TMAP steaks was mainly attributed to the CO-inhibitory effect on the off-odor VOCs production correlated with B. thermosphacta, Pseudomonas and Serratia growth.
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http://dx.doi.org/10.1016/j.meatsci.2022.108861DOI Listing
September 2022

Adiponectin Reduces Lipid Content in Chicken Myoblasts by Activating AMPK Signaling Pathway.

Biosci Rep 2022 May 23. Epub 2022 May 23.

Shandong Agricultural University, Tai'an, China.

Studies in mammals have shown that adiponectin is secreted mainly by adipocytes, and it plays a crucial role in glucose and lipid metabolism in muscles. Clarifying the crosstalk role of adiponectin between adipose tissue and skeletal muscle tissue is very important for internal homeostasis. The glucose and lipid metabolism of chicken is different from that of mammals, and the role of adiponectin in chickens is unclear. Therefore, it is of great significance to study the effect and mechanism of adiponectin on lipid metabolism in chickens. In this study, the regulating effect of adiponectin on lipid metabolism in chicken myoblasts was explored by adding a certain concentration of exogenous recombinant adiponectin. Results showed that adiponectin reduced intracellular lipid content, increasing the mRNA expression of adiponectin receptor and cellular uptake of glucose and fatty acids. In addition, adiponectin activated the 5' adenosine monophosphate activated protein kinase (AMPK) signaling pathway. The above results suggested that adiponectin reduced intracellular lipid content, mainly by binding to adiponectin receptor, activating AMPK pathway, increasing cellular uptake of glucose and fatty acids, and promoting lipid oxidation.
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http://dx.doi.org/10.1042/BSR20212549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171728PMC
May 2022

Prediction of Refracturing Effect of Tight Gas Reservoirs Based on Bayesian Inversion Algorithm.

Comput Intell Neurosci 2022 11;2022:7593526. Epub 2022 May 11.

PetroChina Qinghai Oilfield Company, Dunhuang, Gansu 736202, China.

As a key technology for tight gas stimulation, refracturing plays an important role in tight gas development. In the production process of tight gas wells, the reservoir or fracturing process may cause the hydraulic fractures to gradually fail and the production to continuously decrease. In order to restore the productivity of a single well, it is necessary to refract the well to reopen the failed fractures or fracturing. Reasonable refracturing timing and optimization of refract fracture parameters are important guarantees to ensure the benefits of refracturing in tight gas wells, and relevant research on it can provide theoretical and technical guidance for field construction design. Based on the inverse problem of the dynamic prediction model of tight gas well productivity, this paper proposes an inversion method of formation and fracture parameters before refracturing based on Bayesian inversion algorithm. Finally, based on the geology and development data of the fractured wells in the Sulige gas field, the field application of refracting well selection, determination of refracting reasonable timing, and prediction of refracting effect is carried out. The actual production data are compared, and it is shown that this method can provide theoretical guidance for high-efficiency production-increasing construction on-site.
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http://dx.doi.org/10.1155/2022/7593526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117071PMC
May 2022

Highly multiplexed immune repertoire sequencing links multiple lymphocyte classes with severity of response to COVID-19.

EClinicalMedicine 2022 Jun 14;48:101438. Epub 2022 May 14.

Roche Sequencing Solutions Pleasanton, CA 94588, United States.

Background: Disease progression of subjects with coronavirus disease 2019 (COVID-19) varies dramatically. Understanding the various types of immune response to SARS-CoV-2 is critical for better clinical management of coronavirus outbreaks and to potentially improve future therapies. Disease dynamics can be characterized by deciphering the adaptive immune response.

Methods: In this cross-sectional study we analyzed 117 peripheral blood immune repertoires from healthy controls and subjects with mild to severe COVID-19 disease to elucidate the interplay between B and T cells. We used an immune repertoire Primer Extension Target Enrichment method (immunoPETE) to sequence simultaneously human leukocyte antigen (HLA) restricted T cell receptor beta chain (TRB) and unrestricted T cell receptor delta chain (TRD) and immunoglobulin heavy chain (IgH) immune receptor repertoires. The distribution was analyzed of TRB, TRD and IgH clones between healthy and COVID-19 infected subjects. Using McFadden's Adjusted R2 variables were examined for a predictive model. The aim of this study is to analyze the influence of the adaptive immune repertoire on the severity of the disease (value on the World Health Organization Clinical Progression Scale) in COVID-19.

Findings: Combining clinical metadata with clonotypes of three immune receptor heavy chains (TRB, TRD, and IgH), we found significant associations between COVID-19 disease severity groups and immune receptor sequences of B and T cell compartments. Logistic regression showed an increase in shared IgH clonal types and decrease of TRD in subjects with severe COVID-19. The probability of finding shared clones of TRD clonal types was highest in healthy subjects (controls). Some specific TRB clones seems to be present in severe COVID-19 (Figure S7b). The most informative models (McFadden´s Adjusted R2=0.141) linked disease severity with immune repertoire measures across all three cell types, as well as receptor-specific cell counts, highlighting the importance of multiple lymphocyte classes in disease progression.

Interpretation: Adaptive immune receptor peripheral blood repertoire measures are associated with COVID-19 disease severity.

Funding: The study was funded with grants from the Berlin Institute of Health (BIH).
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http://dx.doi.org/10.1016/j.eclinm.2022.101438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106482PMC
June 2022

A newly-constructed bifunctional bacterial consortium for removing butyl xanthate and cadmium simultaneously from mineral processing wastewater: Experimental evaluation, degradation and biomineralization.

J Environ Manage 2022 Aug 16;316:115304. Epub 2022 May 16.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, Beijing, 100083, China.

Due to the technological limitations associated with beneficiation technology, large amounts of flotation reagents and heavy metals remain in mineral processing wastewater. Unfortunately, however, no treatment methods are available to mitigate the resulting pollution by them. In this study, a bacterial consortium SDMC (simultaneously degrade butyl xanthate and biomineralize cadmium) was constructed in an effort to simultaneously degrade butyl xanthate (BX) and biomineralize cadmium (Cd) by screening and domesticating two different bacterial species including Hypomicrobium and Sporosarcina. SDMC is efficient in removing the combined pollution due to BX and Cd with a 100% degradation rate for BX and 99% biomineralization rate for Cd within 4 h. Besides, SDMC can tolerate high concentrations of Fe(III) (0-40 mg/L). It has an excellent ability to utilize Fe(III) for enhanced removal of the combined pollutants. SDMC can effectively remove pollutants with a pH range of 6-9. Further, we discussed pathways for potential degradation and biomineralization: Cd(BX)-Cd, BX; BX-CS, butyl perxanthate (BPX); Cd-(Ca0.67,Cd0.33)CO. The removal of the combined pollutants primarily entails decomposition, degradation, and biomineralization, C-O bond cleavage, and microbially induced carbonate precipitation (MICP). SDMC is a simple, efficient, and eco-friendly bifunctional bacterial consortium for effective treatment of BX-Cd combined pollution in mineral processing wastewater.
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http://dx.doi.org/10.1016/j.jenvman.2022.115304DOI Listing
August 2022

Dietary Energy and Protein Levels During the Prelay Period on Production Performance, Egg Quality, Expression of Genes in Hypothalamus-Pituitary-Ovary Axis, and Bone Parameters in Aged Laying Hens.

Front Physiol 2022 28;13:887381. Epub 2022 Apr 28.

Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, China.

Nutrition during the pre-lay period takes effect on the production performance in the laying flock. This study evaluated the effects of dietary energy and protein levels in pre-lay diet on performance during the whole laying period and the egg quality, bone quality, and mRNA expression of hypothalamus-pituitary-gonadal (HPG) axis-related genes of hens at the end of the laying cycle. A total of 1,856 15-wk old Hy-Line brown pullets were randomly assigned to one of the four dietary treatments: using a 2 × 2 factorial arrangement with 2 energy levels (2,700 and 2,800 kcal/kg ME, respectively) and 2 protein levels (15 and 16.5% CP, respectively). Pullets were fed from 15 to 20 wk and from 20 wk onward, fed with a similar laying diet till 72 wk of age. At 72 wk, the expression of genes in the hypothalamus, pituitary, ovarian, and follicles and bone quality was evaluated. At 72wk, there were no differences in production performance, BW, organ index, and ovarian parameters among the dietary treatments. High-CP diet increased the egg shape index and eggshell thickness ( < 0.05), but the eggshell breaking strength, Haugh unit, and albumen height did not differ among the treatments. Neither dietary energy nor protein level took an effect of bone quality. Low-energy diet increased the mRNA expression of gonadotropin-releasing hormone-1 () in the hypothalamus ( < 0.05). The mRNA expression level of estrogen receptor-1 () in the hypothalamus and ovary was elevated by the 2,700 ME-15%CP diet ( < 0.05). The expression of cytochrome family 17 subfamily A polypeptide 1 () in the large white follicle (LWF), small yellow follicles (SYF) and dominant follicle (DF) was decreased by the 2,800 kcal/kg diet ( < 0.05). These results indicate that the prelay diet had no influence on the production performance but had minimal effect on the eggshell characteristics and bone parameters. These results suggest that the energy and protein level of the prelay diet changes the expression of HPG axis-related genes of hens around the end of the laying cycle without changing the circulating sex hormone profile. The effect of prelay diet on the endocrinal adjustment at the end of the laying cycle needs to be investigated further.
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http://dx.doi.org/10.3389/fphys.2022.887381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096247PMC
April 2022

Endophyte Pseudomonas putida enhanced Trifolium repens L. growth and heavy metal uptake: A promising in-situ non-soil cover phytoremediation method of nonferrous metallic tailing.

Chemosphere 2021 Jun 29;272:129816. Epub 2021 Jan 29.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China; Beijing Key Laboratory on Resource-Oriented Treatment of Industrial Pollutants, Beijing, 100083, China.

Non-soil cover phytoremediation is the most promising method for high heavy metal contaminated, pH imbalanced and oligotrophic tailing remediation. In this study, a promising method of tailing non-soil cover phytoremediation by endophyte assisting Trifolium repens L. was established. Endophytic Pseudomonas putida strain RE02, with great heavy metal detoxification ability, could colonize in both rhizosphere and endosphere of roots. With RE02 inoculation, the germination percentage of Trifolium repens L. seeds was improved form 40.33%, 45.33% and 56.67%-60.00%, 57.00% and 73.33% in 20 mg/kg Cd, 20 mg/kg Cr and 100 mg/kg Pb contained tailing. The LC50 (concentrations causing 50% mortality of seedlings) and IC50 (concentrations inhibiting the dry biomass by 50%) of Cd, Cr and Pb increased by 6.62, 4.87, 6.27, 4.28, 22.18 and 22.63 mg/kg respectively. Moreover, RE02 inoculation improved soil fertility that the available P and available K was dramatically enhanced in endophyte inoculated groups. Thus, plant NPK concentration was significantly enhanced by 16.72%, 30.55% and 3.81% respectively, and the total heavy metal uptake by 30.03-574.58%. Taken together, Trifolium repens L. successfully grew and developed in heavy metal contaminated, oligotrophic and pH imbalanced tailing, realizing non-soil cover phytoremediation by RE02 inoculation. Overall, this study provided a feasible and promising method for in-situ non-soil cover phytoremediation of tailing, laying a foundation for ecological restoration of tailing.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129816DOI Listing
June 2021

Metabolic Dysfunction-associated Fatty Liver Disease is Associated with Greater Impairment of Lung Function than Nonalcoholic Fatty Liver Disease.

J Clin Transl Hepatol 2022 Apr 4;10(2):230-237. Epub 2022 Jan 4.

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Background And Aims: We compared lung function parameters in nonalcoholic fatty liver disease (NAFLD) and metabolic dysfunction-associated fatty liver disease (MAFLD), and examined the association between lung function parameters and fibrosis severity in MAFLD.

Methods: In this cross-sectional study, we randomly recruited 2,543 middle-aged individuals from 25 communities across four cities in China during 2016 and 2020. All participants received a health check-up including measurement of anthropometric parameters, biochemical variables, liver ultrasonography, and spirometry. The severity of liver disease was assessed by the fibrosis (FIB)-4 score.

Results: The prevalence of MAFLD was 20.4% (=519) and that of NAFLD was 18.4% (=469). After adjusting for age, sex, adiposity measures, smoking status, and significant alcohol intake, subjects with MAFLD had a significantly lower predicted forced vital capacity (FVC, 88.27±17.60% vs. 90.82±16.85%, <0.05) and lower 1 s forced expiratory volume (FEV, 79.89±17.34 vs. 83.02±16.66%, <0.05) than those with NAFLD. MAFLD with an increased FIB-4 score was significantly associated with decreased lung function. For each 1-point increase in FIB-4, FVC was diminished by 0.507 (95% CI: -0.840, -0.173, =0.003), and FEV was diminished by 0.439 (95% CI: -0.739, -0.140, =0.004). The results remained unchanged when the statistical analyses was performed separately for men and women.

Conclusions: MAFLD was significantly associated with a greater impairment of lung function parameters than NAFLD.
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http://dx.doi.org/10.14218/JCTH.2021.00306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039714PMC
April 2022

Insight into soilless revegetation of oligotrophic and heavy metal contaminated gold tailing pond by metagenomic analysis.

J Hazard Mater 2022 08 12;435:128881. Epub 2022 Apr 12.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; Beijing Key Laboratory on Resource-Oriented Treatment of Industrial Pollutants, Beijing 100083, China. Electronic address:

Soilless revegetation is an efficient way for gold tailing remediation, and micro-ecological environments in plant rhizosphere are important for vegetation establishment and pollution removal. In the present study, a field experiment of soilless revegetation has been carried out in a gold tailings pond, and the key genera and functional genes in the plant rhizosphere of gold mine tailings were revealed by metagenomics analysis. Soilless revegetation significantly decreased rhizosphere tailing pH from 8.54 to 7.43-7.87, reduced heavy metal (HM) concentration by 29.81-44.02% and enhanced the nutrient content by 50.30-169.50% averagely. Such improvements were strongly and closely correlated to microbial community and functional gene composition variation. The relative abundance of ecologically beneficial genus such as Actinobacteria (increased 9.7-18.8%) and functional genes involved in carbon, nitrogen and phosphorus cycling such as pyruvate metabolism (relatively increased 8.7-15.0%), assimilatory (increased to 1.44-2.08 times), phosphate ester mineralization (increased to 1.12-1.29 times) and phosphate transportation (increased to 1.28-1.85 times) were significantly increased. Moreover, the relative abundance of most As and Zn resistance genes were reduced, which may relate to the decrease of As and Zn concentration in the rhizosphere tailings. These results revealed the correlation among HM concentrations, microbial composition and functional genes, and provided clear strategies for improving gold mine tailing ecological restoration efficiency.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128881DOI Listing
August 2022

Predictive value of serum adiponectin and hemoglobin levels for vascular cognitive impairment in ischemic stroke patients.

Pak J Med Sci 2022 Mar-Apr;38(3Part-I):705-710

Lifen Huang, Department of Neurology, The Second People's Hospital of Lishui, Lishui 323000, Zhejiang Province, China.

Objectives: To investigate the potential predictive value of serum adiponectin (APN) and hemoglobin (Hb) levels for the occurrence of vascular cognitive impairment in ischemic stroke patients.

Methods: Eighty ischemic stroke patients, admitted to our hospital between June 2019 and November 2020, were retrospectively divided into no cognitive impairment (NCI) group (n=43) and cognitive impairment (CI) group (n=37) based on Montreal Cognitive Assessment (MoCA) scale scoring at three months follow-up. ELISA was used to assess serum Hb and APN levels and receiver operating characteristic (ROC) curves were created to evaluate correlation.

Results: Serum APN and Hb levels were lower in the vascular cognitive impairment group compared to non-impaired counterparts. Pearson correlation analysis showed that both APN and Hb levels were positively correlated with MoCA scores. Area under curve analysis indicated predictive value for serum APN and Hb for predicting cognitive impairment in ischemic stroke patients.

Conclusion: Serum APN and Hb levels in ischemic stroke patients have value for predicting vascular cognitive impairment and may be suitable for helping dictate treatment planning.
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http://dx.doi.org/10.12669/pjms.38.3.5204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9002452PMC
August 2021

Endophyte colonization enhanced cadmium phytoremediation by improving endosphere and rhizosphere microecology characteristics.

J Hazard Mater 2022 07 1;434:128829. Epub 2022 Apr 1.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; Beijing Key Laboratory on Resource-Oriented Treatment of Industrial Pollutants, Beijing 100083, China. Electronic address:

This study investigated the phytoremediation efficiency of Cd-contaminated soils by hyperaccumulator P. acinosa and its endophyte B. cereus, and evaluated the variation of rhizosphere/endosphere microecology characteristics. The result showed that endophyte PE31, which could successfully colonize on P. acinosa root, increased plant Cd uptake by 42.90% and 28.85% in low and high Cd contaminated soils by promotion of plant biomass and Cd concentration in plant tissues. The improved phytoremediation may attribute to the endophyte inoculation, which significantly improved the bioavailable heavy metal (HM) percentage, nutrient cycling related enzyme activities and nutrient contents including available potassium, phosphorus and organic matter. Additionally, the relative abundance beneficial bacteria Bacillus (significantly increased by 81.23% and 34.03% in the endosphere, and by 4.86% and 8.54% in rhizosphere in low and high Cd contaminated soils) and Lysobacter, showed positive and close correlation with plant growth and HM accumulation. These results indicated that endophyte inoculation could reshape rhizosphere and endosphere microecology characteristics, which enhanced the potential for phytoremediation of Cd contaminated soils.
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http://dx.doi.org/10.1016/j.jhazmat.2022.128829DOI Listing
July 2022
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