Publications by authors named "Hai Huang"

785 Publications

Surgical Treatment of Vertical Shear Pelvic Fracture Associated with a Lumbosacral Plexus Injury through the Lateral-Rectus Approach: Surgical Techniques and Preliminary Outcomes.

Orthop Surg 2022 Jun 30. Epub 2022 Jun 30.

Department of Traumatic Surgery, Center for Orthopaedic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.

Objective: To examine the surgical techniques and preliminary outcomes of the lateral rectus approach (LRA) for treating vertical shear (VS) pelvic fracture associated with lumbosacral plexus (LSP) injury.

Methods: This study was a retrospective trial. From August 2010 to October 2017, 29 patients with VS pelvic fractures involving LSP injury who were treated with the LRA were included in this study. The patients were 18-61 years old, with a mean age of 36.2 years. All patients underwent neurolysis, open reduction, and internal fixation (ORIF) through the LRA. The fracture reduction was evaluated using the Matta criteria, and the neural recovery was evaluated by muscle strength grading proposed by the British Medical Research Council (BMRC).

Results: All 29 patients underwent the surgery successfully. The mean operating time was 155.2 ± 32.1 min (range: 105-220 min). The mean operative blood loss was 1021.4 ± 363.4 mL (range: 400-2000 mL). All patients were followed-up for at least 24 months (mean, 32.8 ± 13.5 months; range: 24-96 months). According to the Matta criteria, there were 17 excellent cases, nine good cases, and three fair cases in 29 patients. The ratio of excellent-to-good cases was 89.66%. According to the criteria of the Nerve Injuries Committee of the British Medical Research Council (BMRC), the recovery of nerve and muscle strength achieved to M5 (full recovery of neurological symptoms) was 14 cases, M4 (fine recovery of neurological symptoms), seven cases; M1, M2, and M3 (partial recovery of neurological symptoms), five cases, and M0 (no recovery of neurological symptoms), three cases.

Conclusions: LRA is a safe and feasible surgical approach for treating VS pelvic fractures with LSP injury, which can be used to perform nerve exploration and release from the front, reduce the fracture, and fix it with the anterior iliac plates and/or sacroiliac screws.
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http://dx.doi.org/10.1111/os.13359DOI Listing
June 2022

Editorial: Hearing Loss: From Pathogenesis to Treatment.

Front Cell Neurosci 2022 10;16:907483. Epub 2022 Jun 10.

Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, United States.

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http://dx.doi.org/10.3389/fncel.2022.907483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231520PMC
June 2022

Wushu Routine Movement and Diagnosis Based on Deep Learning and Symmetric Difference Algorithm.

Comput Intell Neurosci 2022 17;2022:1615923. Epub 2022 Jun 17.

Department of Physical Education, Handan University, Handan 056000, Hebei, China.

Wushu is one of the traditional cultural symbols of the Chinese nation. It is also one of the most popular sports activities among the people. With the attention and love of contemporary people to sports activities, Wushu is also constantly developing and innovating. The requirements for professional martial arts routines of martial arts athletes are higher than ever. The development of martial arts has also made martial arts competitions more intense, and often a small detail of martial arts movements can determine the success or failure of the competition. Therefore, various Wushu teams pay more and more attention to the analysis and diagnosis of Wushu routines. It ensures that coaches and athletes can obtain more quantitative indicators of technical movement training. The analysis and diagnosis of martial arts routines are inseparable from the support of reliable science and technology and related algorithms. This article aims to study the analysis and diagnosis of martial arts routines based on deep learning and symmetric difference algorithm. It combines deep learning and symmetric difference algorithm to analyze and diagnose martial arts routines. The article concludes that the level of martial arts routines of martial arts athletes has the greatest influence on their martial arts competition performance, and its comprehensive influence index is as high as 4.3.
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http://dx.doi.org/10.1155/2022/1615923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232334PMC
June 2022

Prognostic Value of Cancer-Associated Fibroblast-Related Gene Signatures in Hepatocellular Carcinoma.

Front Endocrinol (Lausanne) 2022 6;13:884777. Epub 2022 Jun 6.

Department of General Surgery, Hangzhou Traditional Chinese Medicine (TCM) Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, China.

Hepatocellular carcinoma (HCC) is a global health challenge with an increasing incidence worldwide. Cancer-associated fibroblasts (CAFs) function critically in HCC initiation and development. However, the prognostic significance of CAF-related gene signatures in HCC remains unknown. Therefore, the specific functions of CAF-related genes in HCC were investigated to help develop potential therapeutic strategies. In this study, CAF-related genes were screened from three CAF-related gene sets. HCC data from the Gene Expression Omnibus (GEO) database was applied to verify the screened CAF-related genes. Cluster analysis was used to identify clusters based on the expression pattern of CAF-related genes and two identified clusters were found to have a significant difference in overall survival (OS) and progression free intervals (PFI). The prognosis of HCC patients was predicted using the prognostic risk score model developed based on HCC data from The Cancer Genome Atlas (TCGA) databases. High-risk group patients had a worse OS than those in low-risk group in TCGA. These results were validated in International Cancer Genome Consortium (ICGC) database. Moreover, combining the clinicopathological characteristics related to prognosis with the model, a nomogram was built for a more accurate prediction of OS of HCC patients. In addition, analyses of immune infiltration characteristics of tumor microenvironment (TME), chemosensitivity, and immunotherapy response were conducted to further evaluate the prognostic value of CAF-related genes. Patients with low-risk scores were found to have higher chemosensitivity to cisplatin, doxorubicin, and sorafenib. Individuals with high-risk scores were found with a higher expression of most immune checkpoints which indicated patients with high-risk scores may benefit more from treatment with immune checkpoint inhibitors. Furthermore, a correlation between immune infiltration characteristics of TME and patients with different risk levels was found. These findings provide a possibility for the further development of personalized treatments in HCC.
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http://dx.doi.org/10.3389/fendo.2022.884777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207215PMC
June 2022

Long-term and efficient inhibition of hepatitis B virus replication by AAV8-delivered artificial microRNAs.

Antiviral Res 2022 Jun 19;204:105366. Epub 2022 Jun 19.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. Electronic address:

Chronic hepatitis B virus (HBV) infection remains a global health problem and current treatments are insufficient due to immune tolerance to hepatitis B surface antigen (HBsAg). RNA interference (RNAi) is a more promising approach for antiviral therapy. Here, 17 single artificial microRNAs (amiRNAs) targeting the highly conserved regions of HBV genome were screened to inhibit HBV replication. In addition, we compared three tandem amiRNAs, each containing 3 different amiRNAs, out of which amiRNA135 was selected to be studied in detail. In vitro data showed that amiRNA135 significantly inhibited the replication of different HBV genotypes (including resistant and mutant). In vivo study was carried out by adeno-associated virus 8-mediated gene delivery, we found that the anti-HBV effects of AAV8-amiRNA135 were time and dose-dependent. Serum HBsAg and HBeAg in high dose groups were significantly reduced at 7 days after a single intravenous vector injection, and maintained at low levels throughout a 15-month experiment. Immunohistochemical staining and HBV core particle DNA analysis confirmed that HBV replication in the liver was strongly inhibited by AAV8-amiRNA135. Taken together, our data suggest that AAV8-mediated trimeric amiRNA expression is a promising therapeutic approach for chronic HBV infection.
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http://dx.doi.org/10.1016/j.antiviral.2022.105366DOI Listing
June 2022

Poly(β-cyclodextrin)/platinum prodrug supramolecular nano system for enhanced cancer therapy: Synthesis and in vivo study.

Carbohydr Polym 2022 Sep 4;292:119695. Epub 2022 Jun 4.

School of Biomedical Engineering; State Key Laboratory of Oncology in South China, Sun Yat-sen University, Guangzhou 510006, China. Electronic address:

The use of cisplatin is restricted by systemic toxicity and drug resistance. Supramolecular nano-drug delivery systems involving drugs as building blocks circumvent these limitations promisingly. Herein, we describe a novel supramolecular system [Pt(IV)-SSNPs] based on poly(β-cyclodextrin), which was synthesized for efficient loading of adamantly-functionalized platinum(IV) prodrug [Pt(IV)-ADA] via the host-guest interaction between β-cyclodextrin and adamantyl. Pt(IV)-ADA can be converted to active cisplatin in reducing environment in cancer cells, which further reduces systemic toxicity. The introduction of the adamantane group-tethered mPEG endowed the Pt(IV)-SSNPs with a longer blood circulation time. In vitro assays exhibited that the Pt(IV)-SSNPs could be uptaken by CT26 cells, resulting in cell cycle arrest in the G2/M and S phases, together with apoptosis. Furthermore, the Pt(IV)-SSNPs showed effective tumor accumulation, better antitumor effect, and negligible cytotoxicity to major organs. These results indicate that supramolecular nanoparticles are a promising platform for efficient cisplatin delivery and cancer treatment.
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http://dx.doi.org/10.1016/j.carbpol.2022.119695DOI Listing
September 2022

New strategy for clinical etiologic diagnosis of acute ischemic stroke and blood biomarker discovery based on machine learning.

RSC Adv 2022 May 16;12(23):14716-14723. Epub 2022 May 16.

Center for Clinical Laboratories, The Affiliated Hospital of Guizhou Medical University Guiyang 550014 China.

Acute ischemic stroke (AIS) is a syndrome characterized by high morbidity, prevalence, mortality, recurrence and disability. The longer the delay before proper treatment of a stroke, the greater the likelihood of brain damage and disability. Computed tomography and nuclear magnetic resonance are the primary choices for fast diagnosis of AIS in the early stage, which can provide certain information about infarction location and degree, and even the vascular distribution of lesions responsible for strokes. However, this is quite difficult to achieve in small clinics or at-home diagnoses. Hematology tests could quickly obtain a large number of pathology-related indicators, and offer an effective method for rapid AIS diagnosis when combined with the machine learning technique. To explore a reliable, predictable method for early clinical etiologic diagnosis of AIS, a retrospective study was deployed on 456 AIS patients at the early stage and 28 reference subjects without the symptoms of AIS, by means of the selected significant traits amongst 64 clinical and blood traits in conjunction with powerful machine learning strategies. Five representative biomarkers were closely related to cardioembolic (CE), 22 to large artery atherosclerosis (LAA), and 15 to small vessel occlusion (SVO) strokes, respectively. With these biomarkers, different etiologic subtypes of stroke patients were determined with high accuracy of >0.73, sensitivity of >0.73, and specificity of >0.70, which was comparable to the accuracy obtained in the emergency department by clinical diagnosis. The proposed method may offer an alternative strategy for the etiologic diagnosis of AIS at the early stage when integrating significant blood traits into machine learning.
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http://dx.doi.org/10.1039/d2ra02022jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109259PMC
May 2022

Enantioselective synthesis of triarylmethanes organocatalytic transfer hydrogenation of -quinone methides.

Chem Commun (Camb) 2022 Jun 23;58(51):7128-7131. Epub 2022 Jun 23.

Jiangsu Key Laboratory of Advanced Catalytic Materials & Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.

A new organocatalytic asymmetric method for the synthesis of enantioenriched triarylmethanes is developed. Different from the conventional approaches featuring asymmetric arylation, the present study employs asymmetric reduction C-H bond formation as the key step. This approach does not require the presence of a heteroaryl ring or the presynthesis of unstable -quinone methides. Instead, the stable racemic triarylmethanols were used as substrates for the generation of the intermediates with a suitable chiral phosphoric acid catalyst.
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http://dx.doi.org/10.1039/d2cc01996eDOI Listing
June 2022

Catalytic Asymmetric Allylic Substitution/Isomerization with Central Chirality Transposition.

Org Lett 2022 Jun 3;24(23):4246-4251. Epub 2022 Jun 3.

Jiangsu Key Laboratory of Advanced Catalytic Materials & Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.

We have developed a catalytic asymmetric allylic substitution/isomerization process with central chirality transposition. This process takes advantage of the ambident reactivity of the 2-indole imine methide generated from racemic tertiary indolylmethanols. The use of a suitable chiral phosphoric acid catalyst and an -directing group allowed regioselective formation a C-C bond at the 3 position but enantiocontrolled construction of a stereogenic center at the 2-benzylic position.
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http://dx.doi.org/10.1021/acs.orglett.2c01559DOI Listing
June 2022

Hyaluronic acid-based glucose-responsive antioxidant hydrogel platform for enhanced diabetic wound repair.

Acta Biomater 2022 May 29. Epub 2022 May 29.

School of Biomedical Engineering, Sun Yat-sen University, Shenzhen 518107, PR China. Electronic address:

Hyaluronic acid (HA)-based antioxidant hydrogels have achieved remarkable results in diabetic wound repair. However, the realization of their glucose-responsive antioxidant functions remains a significant challenge. In this study, we modified hyaluronic acid methacrylate (HAMA) with phenylboronic acid (PBA) and developed a glucose-responsive HA derivative (HAMA-PBA). A glucose-responsive HAMA-PBA/catechin (HMPC) hydrogel platform was then fabricated by forming a borate ester bond between HAMA-PBA and catechin. The results showed that the HMPC hybrid hydrogel not only had a three-dimensional network structure and Young's modulus similar to those of skin tissue, but also possessed biocompatibility. The HMPC hydrogel also showed unique glucose-responsive catechin release behavior and remarkable antioxidant capability, which could effectively eliminate intracellular reactive oxygen species and protect cells from oxidative stress damage (increased superoxide dismutase activity, stabilized reduced glutathione/oxidized glutathione ratio, and reduced malondialdehyde content). Additionally, in vitro and in vivo experimental results showed that the HMPC hydrogel effectively promoted angiogenesis (enhanced VEGF and CD31 expression) and reduced inflammatory responses (decreased IL-6 level and increased IL-10 level), thus rapidly repairing diabetic wounds (within three weeks). This was a significant improvement as compared to that observed for the untreated control group and the HMP hydrogel group. These results indicated the potential for the application of the HMPC hydrogel for treating diabetic wounds. STATEMENT OF SIGNIFICANCE: At present, the delayed closure rate of diabetic chronic wounds caused by excessive reactive oxygen species (ROS) remains a worldwide challenge. Hyaluronic acid (HA)-based antioxidant hydrogels have made remarkable achievements in diabetic wound repair; however, the realization of their glucose-responsive antioxidant functions is a tough challenge. In this work, we developed a novel HA-based hydrogel platform with glucose-responsive antioxidant activity for rapid repair of diabetic wounds. In vitro and in vivo experimental results showed that the HMPC hydrogel could effectively promote angiogenesis (enhanced VEGF and CD31 expression) and reduce inflammatory response (decreased IL-6 level and increased IL-10 level), thus rapidly repairing diabetic wounds (within 3 weeks). These results indicated the potential of the HMPC hydrogel for application in diabetic wound treatment.
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http://dx.doi.org/10.1016/j.actbio.2022.05.047DOI Listing
May 2022

Clinical characteristics of pneumonia patients of long course of illness infected with SARS-CoV-2.

Open Med (Wars) 2022 18;17(1):947-954. Epub 2022 May 18.

Department of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Epidemiological and clinical characteristics of patients with COVID-19 have been reported in the last two years. A few studies reported clinical course of illness of median 22 days, including viral shedding of median 20 days, but there are several cases with a longer time of viral shedding. In this study, we included four cases with a longer illness course of more than 40 days who had been discharged or still in hospital by March 15, 2020. Demographic, clinical treatment, and laboratory data, including serial samples for viral RNA detection, were extracted from electronic medical records. We described the epidemiological and clinical characteristics and the course of viral shedding. Two patients had comorbidity, one with hypertension and the other with diabetes. We found smoking was not an independent risk factor. D-dimer maybe related to the severity of illness but not to the course of the illness. Nucleic acid detection suggested that maybe more sampling sites represented more virus replication sites and longer course of illness. In this study we found some non-critical severe relatively young patients whose character was different from former studies described to provide a basis for reference to assess the risk of transmission and the isolation duration of patients.
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http://dx.doi.org/10.1515/med-2022-0465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123299PMC
May 2022

15-Month Health Outcomes and the Related Risk Factors of Hospitalized COVID-19 Patients From Onset: A Cohort Study.

Front Med (Lausanne) 2022 11;9:854788. Epub 2022 May 11.

Department of Gastroenterology, Changzheng Hospital, Naval Medical University, Shanghai, China.

Objective: The long-term impact of COVID-19 on patient health has been a recent focus. This study aims to determine the persistent symptoms and psychological conditions of patients hospitalized with COVID-19 15 months after onset, that patients first developed symptoms. The potential risk factors were also explored.

Methods: A cohort of COVID-19 patients discharged from February 20, 2020 to March 31, 2020 was recruited. Follow-ups were conducted using validated questionnaires and psychological screening scales at 15 months after onset to evaluate the patients' health status. The risk factors for long-term health impacts and their associations with disease severity was analyzed.

Findings: 534 COVID-19 patients were enrolled. The median age of the patients was 62.0 years old (IQR 52.0-70.0) and 295 were female (55.2%). The median time from onset to follow-up was 460.0 (451.0-467.0) days. Sleep disturbance (18.5%, 99/534) and fatigue (17.2%, 92/534) were the most common persistent symptoms. 6.4% (34/534) of the patients had depression, 9.2% (49/534) were anxious, 13.0% (70/534) had insomnia and 4.7% (25/534) suffered from post-traumatic stress disorder (PTSD). Multivariate adjusted logistic regression analysis showed that glucocorticoid use during hospitalization (OR 3.58, 95% CI 1.12-11.44) was significantly associated with an increased risk of fatigue. The OR values for anxiety and sleep disorders were 2.36 (95% CI 1.07-5.20) and 2.16 (95% CI 1.13-4.14) in females to males. The OR value of PTSD was 25.6 (95% CI 3.3-198.4) in patients with persistent symptoms to those without persistent symptoms. No significant associations were observed between fatigue syndrome or adverse mental outcomes and disease severity.

Conclusions: 15-month follow-up in this study demonstrated the need of extended rehabilitation intervention for complete recovery in COVID-19 patients.
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http://dx.doi.org/10.3389/fmed.2022.854788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131089PMC
May 2022

USP1 inhibition suppresses the progression of osteosarcoma via destabilizing TAZ.

Int J Biol Sci 2022 1;18(8):3122-3136. Epub 2022 May 1.

Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.

Mutations and altered expression of deubiquitinating enzymes (DUBs) profoundly influence tumor progression. Ubiquitin-specific protease 1 (USP1) is a well-characterized human DUB reportedly overexpressed in and associated with maintaining the mesenchymal stem cell status of osteosarcoma (OS); however, the potential mechanisms of USP1 in OS remain poorly understood. In this study, we identified that USP1 directly interacts with Transcriptional Co-Activator With PDZ-Binding Motif (TAZ) in OS cell lines, and with mechanistic analysis indicating that the anti-OS effects of USP1 inhibition could be partially attributed to TAZ instability, with its reduced nuclear accumulation responsible for a subsequent decrease in the expression of downstream genes associated with the Hippo signaling pathway. Moreover, pharmacological inhibition USP1 by ML323 presented the similar effects on Hippo signaling pathway and suppressed OS growth and metastasis both and . Taken together, our results revealed a novel molecular mechanism underlying the function of USP1 in OS and a potential role of ML323 as a therapeutic strategy for the clinical treatment of OS.
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http://dx.doi.org/10.7150/ijbs.65428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134912PMC
June 2022

Magnesium surface-activated 3D printed porous PEEK scaffolds for osseointegration by promoting angiogenesis and osteogenesis.

Bioact Mater 2023 Feb 18;20:16-28. Epub 2022 May 18.

Department of Orthopaedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710038, China.

Polyetheretherketone (PEEK) has been an alternative material for titanium in bone defect repair, but its clinical application is limited by its poor osseointegration. In this study, a porous structural design and activated surface modification were used to enhance the osseointegration capacity of PEEK materials. Porous PEEK scaffolds were manufactured via fused deposition modeling and a polydopamine (PDA) coating chelated with magnesium ions (Mg) was utilized on the surface. After surface modification, the hydrophilicity of PEEK scaffolds was significantly enhanced, and bioactive Mg could be released. results showed that the activated surface could promote cell proliferation and adhesion and contribute to osteoblast differentiation and mineralization; the released Mg promoted angiogenesis and might contribute to the formation of osteogenic H-type vessels. Furthermore, porous PEEK scaffolds were implanted in rabbit femoral condyles for evaluation of osseointegration. The results showed that the customized three-dimensional porous structure facilitated vascular ingrowth and bone ingrowth within the PEEK scaffolds. The PDA coating enhanced the interfacial osseointegration of porous PEEK scaffolds and the released Mg accelerated early bone ingrowth by promoting early angiogenesis during the coating degradation process. This study provides an efficient solution for enhancing the osseointegration of PEEK materials, which has high potential for translational clinical applications.
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http://dx.doi.org/10.1016/j.bioactmat.2022.05.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123089PMC
February 2023

20 (S)-ginsenoside Rh2 inhibits colorectal cancer cell growth by suppressing the Axl signaling pathway in vitro and in vivo.

J Ginseng Res 2022 May 12;46(3):396-407. Epub 2021 Jul 12.

Department of Animal Science and Biotechnology, ITRD, Kyungpook National University, Sangju, Republic of Korea.

Background: Colorectal cancer (CRC) has a high morbidity and mortality worldwide. 20 (S)-ginsenoside Rh2 (G-Rh2) is a natural compound extracted from , which exhibits anticancer effects in many cancer types. In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 in CRC cells in vitro and in vivo.

Methods: Cell proliferation, migration, invasion, apoptosis, cell cycle, and western blot assays were performed to evaluate the effect of G-Rh2 on CRC cells. In vitro pull-down assay was used to verify the interaction between G-Rh2 and Axl. Transfection and infection experiments were used to explore the function of Axl in CRC cells. CRC xenograft models were used to further investigate the effect of Axl knockdown and G-Rh2 on tumor growth in vivo.

Results: G-Rh2 significantly inhibited proliferation, migration, and invasion, and induced apoptosis and G/G phase cell cycle arrest in CRC cell lines. G-Rh2 directly binds to Axl and inhibits the Axl signaling pathway in CRC cells. Knockdown of Axl suppressed the growth, migration and invasion ability of CRC cells in vitro and xenograft tumor growth in vivo, whereas overexpression of Axl promoted the growth, migration, and invasion ability of CRC cells. Moreover, G-Rh2 significantly suppressed CRC xenograft tumor growth by inhibiting Axl signaling with no obvious toxicity to nude mice.

Conclusion: Our results indicate that G-Rh2 exerts anticancer activity in vitro and in vivo by suppressing the Axl signaling pathway. G-Rh2 is a promising candidate for CRC prevention and treatment.
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http://dx.doi.org/10.1016/j.jgr.2021.07.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120647PMC
May 2022

Metabolic control of progenitor cell propagation during Drosophila tracheal remodeling.

Nat Commun 2022 May 20;13(1):2817. Epub 2022 May 20.

Department of Cell Biology, and Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, 310058, China.

Adult progenitor cells in the trachea of Drosophila larvae are activated and migrate out of niches when metamorphosis induces tracheal remodeling. Here we show that in response to metabolic deficiency in decaying tracheal branches, signaling by the insulin pathway controls the progenitor cells by regulating Yorkie (Yki)-dependent proliferation and migration. Yki, a transcription coactivator that is regulated by Hippo signaling, promotes transcriptional activation of cell cycle regulators and components of the extracellular matrix in tracheal progenitor cells. These findings reveal that regulation of Yki signaling by the insulin pathway governs proliferation and migration of tracheal progenitor cells, thereby identifying the regulatory mechanism by which metabolic depression drives progenitor cell activation and cell division that underlies tracheal remodeling.
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http://dx.doi.org/10.1038/s41467-022-30492-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122933PMC
May 2022

Corrigendum to "Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage?"

Mediators Inflamm 2022 9;2022:9870324. Epub 2022 May 9.

Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Friedrich Schiller University Jena, Drackendorfer Str.1, 07747 Jena, Germany.

[This corrects the article DOI: 10.1155/2010/436145.].
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http://dx.doi.org/10.1155/2022/9870324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110253PMC
May 2022

Electroacupuncture regulates sperm motility in patients with asthenozoospermia: study protocol for a randomized sham-controlled clinical trial.

Minerva Med 2022 May 18. Epub 2022 May 18.

The Seventh People's Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

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http://dx.doi.org/10.23736/S0026-4806.22.08175-7DOI Listing
May 2022

Pelvic reconstruction and lateral prostate capsule sparing techniques improve early continence of robot-assisted radical cystectomy with orthotopic ileal neobladder.

Int Urol Nephrol 2022 Jul 12;54(7):1537-1543. Epub 2022 May 12.

Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yanjiangxi Road, Yuexiu District, Guangzhou, 510120, Guangdong, China.

Purpose: To evaluate urinary outcomes of pelvic construction and lateral capsule sparing techniques in robot-assisted radical cystectomy with orthotopic ileal neobladder (RARC-OIN).

Methods: A total of 107 male patients who underwent RARC-OIN during January 2017 and February 2021 in Sun Yat-sen Memorial Hospital were analyzed retrospectively. Standard RARC-OIN with or without nerve sparing technique was performed in 44 patients (standard group), lateral prostate capsule sparing technique was performed in 20 patients (LCS group), combined pelvic reconstruction (CPR) technique including anterior suspension and posterior reconstruction were performed in 43 patients (CPR group). The urinary function was assessed by the use of pads and the Bladder Cancer Index (BCI). Continence was defined as the use of 0-1 pad during daytime or night-time.

Results: There was no statistical difference between the three groups regarding demographic, perioperative, and pathological data. Continence rates were 6.8, 50.0 and 34.9% for daytime, 4.6, 40.0 and 32.6% for night-time in the standard group, LCS group and CPR group at 1 month post-operation, respectively. Continence rates were 34.1, 80.0 and 69.8% for daytime, 27.3, 75.0 and 65.1% for night-time in the standard group, LCS group and CPR group at 3 month post-operation, respectively. No statistically significant difference was observed in the daytime and night-time continence rates at 12 months.

Conclusions: Lateral capsule-sparing and combined pelvic reconstruction techniques are feasible to improve early daytime and night-time continence rates in RARC with orthotopic neobladder.

Clinical Trial Registration: The trial registration number: ChiCTR2100047606.
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http://dx.doi.org/10.1007/s11255-022-03214-4DOI Listing
July 2022

A dataset of winter wheat aboveground biomass in China during 2007-2015 based on data assimilation.

Sci Data 2022 05 11;9(1):200. Epub 2022 May 11.

School of Atmospheric Sciences, Sun Yat-sen University, Guangzhou, 510245, Guangdong, China.

As a key variable to characterize the process of crop growth, the aboveground biomass (AGB) plays an important role in crop management and production. Process-based models and remote sensing are two important scientific methods for crop AGB estimation. In this study, we combined observations from agricultural meteorological stations and county-level yield statistics to calibrate a process-based crop growth model for winter wheat. After that, we assimilated a reprocessed temporal-spatial filtered MODIS Leaf Area Index product into the model to derive the 1 km daily AGB dataset of the main winter wheat producing areas in China from 2007 to 2015. The validation using ground measurements also suggests the derived AGB dataset agrees well with the filed observations, i.e., the R is above 0.9, and the root mean square error (RMSE) reaches 1,377 kg·ha. Compared to county-level statistics during 2007-2015, the ranges of R, RMSE, and mean absolute percentage error (MAPE) are 0.73~0.89, 953~1,503 kg·ha, and 8%~12%, respectively. We believe our dataset can be helpful for relevant studies on regional agricultural production management and yield estimation.
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http://dx.doi.org/10.1038/s41597-022-01305-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095600PMC
May 2022

Antitumor Effect of Simvastatin in Combination With DNA Methyltransferase Inhibitor on Gastric Cancer GSDME-Mediated Pyroptosis.

Front Pharmacol 2022 20;13:860546. Epub 2022 Apr 20.

Center for Clinical Laboratories, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.

Gasdermin E (GSDME) is one of the executors of pyroptosis, a type of programmed lytic cell death, which can be triggered by caspase-3 activation upon stimulation. Silenced GSDME expression due to promoter hypermethylation is associated with gastric cancer (GC), which is confirmed in the present study by bioinformatics analysis and methylation-specific PCR (MSP) test of GC cell lines and clinical samples. GC cell lines and mouse xenograft models were used to investigate the pyroptosis-inducing effect of the common cholesterol-depleting, drug simvastatin (SIM), allied with upregulating GSDME expression by doxycycline (DOX)- inducible Tet-on system or DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (5-Aza-CdR). Cell viability assessment and xenograft tumour growth demonstrated that the tumour inhibition effects of SIM can be enhanced by elevated GSDME expression. Morphological examinations and assays measuring lactate dehydrogenase (LDH) release and caspase-3/GSDME protein cleavage underlined the stimulation of pyroptosis as an important mechanism. Using short hairpin RNA (shRNA) knockdown of caspase-3 or GSDME, and caspase-3-specific inhibitors, we provided evidence of the requirement of caspase-3/GSDME in the pyroptosis process triggered by SIM. We conclude that reactivating GSDME expression and thereby inducing cancer cell-specific pyroptosis could be a potential therapeutic strategy against GC.
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http://dx.doi.org/10.3389/fphar.2022.860546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065610PMC
April 2022

Octreotide-based therapies effectively protect mice from acute and chronic gastritis.

Eur J Pharmacol 2022 Apr 26:174976. Epub 2022 Apr 26.

State Key Laboratory of Natural Medicines, Key Laboratory of Drug Metabolism, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

Gastritis is a common inflammation of stomach with multiple pathogenesis. This study was designed to investigate the protective effects of oral octreotide (OCT) against ethanol-induced acute gastric injury and H. pylori-induced chronic gastritis via promoting gastric mucosa restoration, reducing gastric acid secretion and inflammation. Male C57BL/6J mice were randomly divided and treated with three doses of OCT (0.5, 2.5, 10 mg/kg) alone or combined respectively with 10 mg/kg omeprazole (OME), 0.2 g/L metronidazole (MTZ)/0.1 g/L clarithromycin (CLR) in drinking water. Oxidative stress analysis, bacterial load analysis, qPCR, gastric histopathology examinations were performed in our study. Ethanol-induced acute gastric ulcer was restored by OCT alone at doses of 2.5 mg/kg, or combined with OME as indicated by markedly reducing Gastrin, Il-6 and Il1b expression through induction of Muc5ac and Occludin, significantly improving hyperacidity and gastric bleeding. As well, OCT combined with MTZ/CLR restored the integrity of gastric mucosa damaged by H. pylori via elevating the expression of Muc5ac and somatostatin receptor 2, decreasing inflammation and increasing the number of chorionic or glands. Besides, OCT is more suitable for long-term medication in the treatment of chronic gastritis than OME. In conclusion, our results proved that the newly developed oral OCT-based therapies were more effective to reverse gastric mucosa damage and inflammation in ethanol and H. pylori infection-induced gastric injury, it is of great significance for supplementing new clinical regimens for the treatment of acute and chronic gastritis.
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http://dx.doi.org/10.1016/j.ejphar.2022.174976DOI Listing
April 2022

Reductive Opening of Oxetanes Catalyzed by Frustrated Lewis Pairs: Unexpected Aryl Migration via Neighboring Group Participation.

Org Lett 2022 May 25;24(17):3259-3264. Epub 2022 Apr 25.

Jiangsu Key Laboratory of Advanced Catalytic Materials & Technology, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.

B(CF) was found to catalyze an unusual double reduction of oxetanes by hydrosilane with aryl migration via neighboring group participation. Control experiments suggested that the phenonium ion serves as the key intermediate. Minor modification of this protocol also led to simple hydrosilylative opening of oxetanes.
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http://dx.doi.org/10.1021/acs.orglett.2c01088DOI Listing
May 2022

Liver-Targeted Nanoparticles Facilitate the Bioavailability and Anti-HBV Efficacy of Baicalin In Vitro and In Vivo.

Biomedicines 2022 Apr 14;10(4). Epub 2022 Apr 14.

Department of Biological Medicines & Shanghai Engineering Research Center of Immunotherapeutics, Fudan University School of Pharmacy, Shanghai 201203, China.

The anti-hepatitis B virus (HBV) efficacy of baicalin (BA) is mediated by HBV-related hepatocyte nuclear factors (HNFs). However, this efficacy is severely limited by the low bioavailability of BA. Therefore, a novel liver-targeted BA liposome was constructed to promote the bioavailability and antiviral ability of BA. The results showed that apolipoprotein A1 (ApoA1)-modified liposomes (BAA1) significantly enhanced BA's cellular uptake and specific distribution in the liver. Furthermore, the substantial inhibitory effects of BAA1 on HBsAg, HBeAg, HBV RNA, and HBV DNA were assessed in HB-infected cells and mice. Western blotting, co-immunoprecipitation, and transcriptomics analysis further revealed that the enhanced anti-HBV efficacy of BAA1 was attributed to the interaction between hepatocyte nuclear factors (HNFs) and estrogen receptors (ERs). Based on the findings, we propose that the ApoA1-modified liposomes aid BA in inhibiting HBV transcription and replication by augmenting its bioavailability and the HNFs-ERs axis.
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http://dx.doi.org/10.3390/biomedicines10040900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9025464PMC
April 2022

Radiologically anatomic measurement analysis for the third sacral foramen and an efficient implantation protocol for sacral neuromodulation.

Neurourol Urodyn 2022 06 19;41(5):1149-1156. Epub 2022 Apr 19.

Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Aims: To define radiologically anatomic measurements for the S3 foramen and develop an efficient and straightforward implantation protocol for sacral neuromodulation (SNM) in a southern Chinese population.

Methods: We retrospectively reviewed 793 adults without sacrococcygeal abnormalities or bony injury. We generated CT-derived three-dimensional volumetric models and collected radiologically anatomic measurements of S3 foramen. A multiple generalized linear regression model was applied to evaluate implantation protocol for SNM.

Results: Height, weight, and sacral height were significantly different between enrolled patients based on sex (p < 0.001 for all), while age and sacral width were similar. The measurements L1, L2, A1, and A2 on both sides were similar, while based on sex, those of L1, A1, A2 showed significant differences [7.02 ± 0.90 (M) vs. 6.10 ± 0.99 cm (F) (p < 0.001); 83.05 ± 6.23 (M) vs. 82.08 ± 7.53° (F) (p = 0.005); and 13.31 ± 5.69 (M) vs. 11.97 ± 4.91° (F) (p < 0.001), respectively]. The multiple generalized linear regression model demonstrated that sex was a common independent factor for estimating L1, L2, A1, A2, while the consistency rate between the estimated model and actual measurements was poor. The measurements L1, L2, A1, and A2 were approximately 7.0 cm (M) versus 6.1 cm (F), 1.8 cm (M) versus 1.8 cm (F), 83° (M) versus 82° (F), and 13° (M) versus 12° (F), respectively.

Conclusions: This retrospective study indicates that the radiologically anatomic measurements for the S3 foramen have unique characteristics. Our study provides an efficient and straightforward implantation protocol for SNM, improving its use in China.
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http://dx.doi.org/10.1002/nau.24933DOI Listing
June 2022

The role of chemotherapy in patients with stage IB gastric adenocarcinoma: a real-world competing risk analysis.

World J Surg Oncol 2022 Apr 17;20(1):123. Epub 2022 Apr 17.

Department of General Surgery, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, #453, Tiyuchang Road, Xihu District, Hangzhou, 310000, Zhejiang Province, China.

Background: This study aimed to investigate the potential effect of adjuvant chemotherapy in patients diagnosed with stage IB gastric adenocarcinoma (GAC).

Method: A total of 1727 patients were included in the Surveillance, Epidemiology, and End Results (SEER) database from 2004 to 2015 and divided into the chemotherapy and no-chemotherapy groups. Then, the methods of Kaplan-Meier analysis, propensity score matching (PSM), and competing risk analysis were implemented.

Results: After PSM, no significant difference was found in the chemotherapy and no-chemotherapy groups in overall survival (OS) (p=0.4) and cancer-specific survival (CSS) (p=0.12) in survival curves. The competing risk analysis presented that the 5-year cumulative incidence of cancer-specific death (CSD) was significantly lower in patients receiving chemotherapy (11.5% vs. 20.8%, p=0.007), while no significant discrepancy was observed in other causes of death (OCD) in both groups (10.6% vs. 10.9%, p=0.474). Multivariable competing risks regression models presented a significant correlation between chemotherapy and CSD (HR, 0.51; 95%CI, 0.31-0.82; p=0.007).

Conclusion: The stage IB GAC patients can benefit from adjuvant chemotherapy based on this competing risk analysis.
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http://dx.doi.org/10.1186/s12957-022-02591-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013453PMC
April 2022

Interleukin-35 promotes Breg expansion and interleukin-10 production in CD19 B cells in patients with ankylosing spondylitis.

Clin Rheumatol 2022 Apr 14. Epub 2022 Apr 14.

Center for Clinical Laboratories, The Affiliated Hospital of Guizhou Medical University, 28 Guiyi Street, Guiyang, 550004, Guizhou, China.

Objective: IL-35 is a potent immunosuppressive and anti-inflammatory cytokine, consisting of a p35 subunit and an Epstein-Barr virus-induced gene 3 (EBI3) subunit, which suppresses CD4 effector T cell proliferation and promotes regulatory T cell (Treg) expansion. However, the effects of IL-35 on regulatory B cells (Bregs) in ankylosing spondylitis (AS) have not been explored. The present study aimed (i) to measure serum IL-35 levels and the percentages of Bregs in the peripheral blood of patients with AS and (ii) to explore their relationships in the pathogenesis of AS.

Methods: A total of 77 patients with AS (AS group), including 47 inactive AS and 30 active AS cases, and 59 healthy controls (HCs) were enrolled into this study. The serum levels of IL-35 and IL-10 were detected by ELISA, and the mRNA levels of p35 and EBI3 were measured by RT-qPCR. The percentages of CD19CD24CD38 and CD19CD24CD27 Bregs and IL-35 receptor (IL-12Rβ2, IL-27Rα and gp130), IL-10, p-STAT1, p-STAT3, and p-STAT4 in CD19 B cells were detected by flow cytometry. The correlations between IL-35 levels and percentages of Bregs were analyzed by determining Pearson's correlation coefficient. The effect of IL-35 on Bregs was determined by mix-culture of recombinant (r) IL-35 with peripheral blood mononuclear cells (PBMCs).

Results: The serum IL-35 and IL-10 levels, p35 and EBI3 mRNA levels, and the percentages of CD19CD24CD38 and CD19CD24CD27 Bregs were significantly lower in AS patients than those in HCs. In addition, the percentages of CD19CD24CD38 and CD19CD24CD27 Bregs in active AS patients were significantly lower than those in inactive AS patients. The serum IL-35 levels were positively correlated with the percentages of CD19CD24CD38 and CD19CD24CD27 Bregs in AS patients. IL-12Rβ2 and IL-27Rα, but not gp130 subunit, were expressed in CD19 B cells in AS patients. RIL-35 could effectively promote CD19CD24CD38 Breg expansion and IL-10 production. Meanwhile, rIL-35 also promoted the expression of IL-12Rβ2 and IL-27Rα and the phosphorylation of STAT1 and STAT3 in CD19 B cells.

Conclusion: These results demonstrated that reduced IL-35 production may be associated with Bregs defects in AS patients. RIL-35 induced the proliferation of CD19CD24CD38 Bregs and IL-10 production, suggesting that IL-35 may serve as a reference for further investigation to develop novel treatments for AS. Key Points • Our study investigated the effects of IL-35 on Bregs in AS patients. • We found the serum IL-35, IL-10 levels, and the percentages of CD19CD24CD38 and CD19CD24CD27 Bregs were significantly lower in AS patients. • The serum IL-35 levels were positively correlated with the percentages of CD19CD24CD38 and CD19CD24CD27 Bregs in AS patients. • Recombinant IL-35 could effectively promote CD19CD24CD38 Breg expansion and IL-10 production.
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http://dx.doi.org/10.1007/s10067-022-06137-8DOI Listing
April 2022

Comparison of the Effects of Intramedullary Nailing and Plate Fixation on Lower-Extremity Deep Vein Thrombosis after Tibial Fractures.

Comput Math Methods Med 2022 1;2022:4852201. Epub 2022 Apr 1.

Department of Orthopedic Trauma, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054 Shaanxi Province, China.

Lower-extremity deep vein thrombosis (DVT) is prone to occur after internal fixation of tibial fractures. This study analyzed the effect of intramedullary nailing (IMN) and plate fixation (PF) on lower-extremity DVT, providing reliable reference and guidance for future clinical treatment of tibial fractures. Sixty-eight patients with tibial fractures admitted to Honghui Hospital, Xi'an Jiaotong University, between February 2019 and October 2020 were selected as research participants, of which 32 cases treated with open reduction and locking-compression plate fixation were assigned to the FP group and 36 cases treated with closed reduction and interlocking IMN were included in the FN group. The two groups were compared regarding the following items: clinical efficacy, operation, rehabilitation, joint function, pain, inflammatory factors (IFs), incidence of adverse reactions (ARs), blood loss, prognosis, and quality of life (QoL). The related factors affecting the occurrence of DVT were analyzed. The results identified no evident difference in the overall response rate between the two groups ( > 0.05). The FN group showed longer operation time, higher incidence of ARs, and better rehabilitation, while there were lower incision length, VAS score, and IF levels ( < 0.05). The results revealed no significant difference in estnimated blood volume(EBV) and the incidence of DVT between the two groups( > 0.05); however, the total blood loss (TBL), hidden blood loss (HBL), and blood transfusion rates in FN group were higher while the visible blood loss (VBL) was lower compared to the FP group ( < 0.05). Logistic regression analysis identified that blood transfusion, VBL, HBL, TBL, and treatment methods were independent risk factors affecting the occurrence of lower-extremity DVT ( < 0.05). In addition, the prognostic QoL was better in the FN group ( < 0.05). Therefore, closed reduction and interlocking IMN are more effective than open reduction and locking-compression plate fixation in the treatment of tibial fractures, but patients are more likely to suffer from lower-extremity DVT. In the future, it is necessary to carefully choose the treatment method in the treatment of tibial fracture patients to ensure their rehabilitation.
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http://dx.doi.org/10.1155/2022/4852201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993547PMC
April 2022

Generic character of charge and spin density waves in superconducting cuprates.

Proc Natl Acad Sci U S A 2022 04 4;119(15):e2119429119. Epub 2022 Apr 4.

Author affiliations: Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Charge density waves (CDWs) have been observed in nearly all families of copper-oxide superconductors. But the behavior of these phases across different families has been perplexing. In La-based cuprates, the CDW wavevector is an increasing function of doping, exhibiting the so-called Yamada behavior, while in Y- and Bi-based materials the behavior is the opposite. Here, we report a combined resonant soft X-ray scattering (RSXS) and neutron scattering study of charge and spin density waves in isotopically enriched La1.8−xEu0.2SrxCuO4 over a range of doping 0.07≤x≤0.20. We find that the CDW amplitude is temperature independent and develops well above experimentally accessible temperatures. Further, the CDW wavevector shows a nonmonotonic temperature dependence, exhibiting Yamada behavior at low temperature with a sudden change occurring near the spin ordering temperature. We describe these observations using a Landau–Ginzburg theory for an incommensurate CDW in a metallic system with a finite charge compressibility and spin-CDW coupling. Extrapolating to high temperature, where the CDW amplitude is small and spin order is absent, our analysis predicts a decreasing wavevector with doping, similar to Y and Bi cuprates. Our study suggests that CDW order in all families of cuprates forms by a common mechanism.
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http://dx.doi.org/10.1073/pnas.2119429119DOI Listing
April 2022

Autophagy and Exosome Coordinately Enhance Macrophage M1 Polarization and Recruitment in Influenza A Virus Infection.

Front Immunol 2022 17;13:722053. Epub 2022 Mar 17.

Department of Biological Medicines & Shanghai Engineering Research Center of Immunotherapeutics, Fudan University School of Pharmacy, Shanghai, China.

Background: Influenza A virus infection results in viral pneumonia, which is often accompanied by the infiltration and recruitment of macrophages, overactivation of inflammatory responses, and obvious cell autophagy and exosome production. However, little is known about the roles of autophagy and exosome production in these inflammatory responses.

Methods: In this study, multiple methods, such as flow cytometry, real-time quantitative reverse transcription-polymerase chain reaction, immune-fluorescence technology, and western blot, were applied to explore the possible effects of autophagy and exosome production by H1N1-infected host cells.

Results: It was observed that a high number of polarized macrophages (CD11b/F4/80/CD86) were recruited to the lung tissues of infected mice, which could be mimicked by tracking the movement of macrophages to H1N1-infected cells (transwell assays). Furthermore, there was some coordinated upregulation of M1 polarization signs (iNOS/Arg-1 bias) as well as autophagy (LC3) and exosome (CD63) biomarkers in the infected macrophages and epithelial cells. Moreover, exosomes extracted from the supernatant of virus-infected cells were shown to promote the recruitment and polarization of more peritoneal macrophages than the normal group. The fluorescence colocalization of LC3-CD63 and the inhibition of autophagy and exosome signaling pathway further revealed that H1N1 infection seemed to sequentially activate the M1 polarization and recruitment of macrophages autophagy-exosome dependent pathway.

Conclusion: Autophagy and exosome production coordinately enhance the M1 polarization and recruitment of macrophages in influenza virus infection, which also provides potential therapeutic targets.
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http://dx.doi.org/10.3389/fimmu.2022.722053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967985PMC
April 2022
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