Publications by authors named "Hafeez Ullah"

15 Publications

  • Page 1 of 1

Appraisal of anti-mycobacterial potential against MDR-MTB in pediatric patients, cytotoxicity and mutagenicity of Aloe vera and Allium sativum.

Pak J Pharm Sci 2021 Jan;34(1(Supplementary)):257-263

Faculty of Pharmacy, University of Central Punjab, Lahore, Pakistan.

The purpose of the studies was to evaluate an in-vitro anti-mycobacterial activity of Aloe vera and Allium sativum against MDR-MTB, their cytotoxicity and mutagenicity. Four extracts of Aloe vera and Allium sativum were prepared by Soxhlet apparatus and their minimum inhibitory concentrations (MIC's) were determined by BACTEC MGIT960 system against multi drug resistant Mycobacterium tuberculosis (MDR-MTB) isolates, collected from pediatric patients. Fractions of Aloe vera and Allium sativum extracts were separated using glass column chromatography, followed by evaluation of cytotoxicity and mutagenicity by tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Ames test, respectively. Out of four extracts, ethanol extracts of Aloe vera and Allium sativum exhibited activity at MIC 5mg/mL to 7mg/mL and 3mg/mL to 5mg/mL, respectively and IC by MTT assay for combination of all fractions were 278.3mcg/100μL and 270.8mcg/100μL and in Ames assay M.I of TA98 were 0.14 and 0.07 and M.I of TA100 were 1.14 and 0.44, respectively. Aloe vera and Allium sativum extracts showed anti-mycobacterial activity against MDR-MTB isolates so, MIC of ethanol extracts of each plant and fractions of column chromatography had been checked. The MTT and Ames tests depicted that ethanol extracts of Aloe vera and Allium sativum were non-cytotoxic and non-mutagenic, and can be used in treatment of patients suffering from MDR-MTB.
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January 2021

Self-healing epoxy coating synthesis by embedment of metal 2-methyl imidazole and acetylacetonate complexes with microcapsules.

Chemosphere 2021 Jul 8;285:131492. Epub 2021 Jul 8.

Department of Chemical and Environmental Engineering, Faculty Science and Engineering, University of Nottingham, Malaysia, 43500, Semenyih, Selangor, Darul Ehsan, Malaysia. Electronic address:

The restoration of mechanical properties is desired for creating the self-healing coatings with no corrosion capabilities. The encapsulation of epoxy resins is limited by various factors in urea and melamine formaldehyde microcapsules. An improved method was developed, where epoxy resin was encapsulated by individual wrapping of poly(melamine-formaldehyde) and poly(urea-formaldehyde) shell around emulsified epoxy droplets via oil-in-water emulsion polymerization method. The synthesized materials were characterized analytically. The curing of the epoxy was achieved by adding the [Ni/Co(2-MI)].2NO as a latent hardener and iron acetylacetonate [Fe(acac)] as a latent accelerator. Isothermal and non-isothermal differential scanning calorimetric analysis revealed lower curing temperature (T = 116 °C) and lower activation energies (Ea ≈ 69-75 kJ/mol). The addition of microcapsules and complexes did not adversely alter the flexural strength and flexural modulus of the epoxy coatings. The adhesion strength of neat coating decreased from 6310.8 ± 31 to 4720.9 ± 60 kPa and percent healing increased from 50.83 to 67.45% in the presence of acetylacetonate complex at 10 wt% of microcapsules.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131492DOI Listing
July 2021

Nexus between energy policy and environmental performance in China: The moderating role of green finance adopted firms.

Environ Sci Pollut Res Int 2021 Jul 5. Epub 2021 Jul 5.

College of Management, Ocean University of China, Qingdao, China.

This study measures the association between resources and the atmosphere; social and environmental aspects of energy production have become critical. In this context, the aim of this research is to explore the mediating effect of renewable energy patents in developing potential frameworks for energy policy viewpoints on the climate. The study took panel data from 2010 to 2017 and used a non-radial data envelopment analysis (DEA) process and panel data model for 30 Chinese provinces. The findings indicate that between 2010 and 2017, the average environmental efficiency index (EPI) of Chinese areas increased by 9.88%. When firms' internal variables are proxied by their commodity (revenue), the relationship term's point approximate coefficient is about 0.05. This magnitude means that a 1% rise in a company's assets will result in a 5% increase is estimated to be about 0.157, implying that a 1% rise in firm leverage is correlated with a 15.7%. Finally, based on the study results, some policy implications were proposed.
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http://dx.doi.org/10.1007/s11356-021-15195-5DOI Listing
July 2021

Differentiation of Brain Tumor Microvasculature From Normal Vessels Using Optical Coherence Angiography.

Lasers Surg Med 2021 Jun 15. Epub 2021 Jun 15.

Department of Biomedical Engineering, The University of Texas Austin, Austin, Texas, 78712, USA.

Background And Objectives: Despite rapid advances and discoveries in medical imaging, monitoring therapeutic efficacy for malignant gliomas and monitoring tumor vasculature remains problematic. The purpose of this study is to utilize optical coherence angiography for vasculature characterization inside and surrounding brain tumors in a murine xenograft brain tumor model. Features included in our analysis include fractional blood volume, vessel tortuosity, diameter, orientation, and directionality.

Study Design/materials And Methods: In this study, five tumorous mice models at 4 weeks of age were imaged. Human glioblastoma cells were injected into the brain and allowed to grow for 4 weeks and then imaged using optical coherence tomography.

Results: Results suggest that blood vessels outside the tumor contain a greater fractional blood volume as compared with vessels inside the tumor. Vessels inside the tumor are more tortuous as compared with those outside the tumor. Results indicate that vessels near the tumor margin are directed inward towards the tumor while normal vessels show a more random orientation.

Conclusion: Quantification of vascular microenvironments in brain gliomas can provide functional vascular parameters to aid various diagnostic and therapeutic studies. © 2021 Wiley Periodicals LLC.
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http://dx.doi.org/10.1002/lsm.23446DOI Listing
June 2021

Nexus between IT capability and green intellectual capital on sustainable businesses: evidence from emerging economies.

Environ Sci Pollut Res Int 2021 Jun 30;28(22):27825-27843. Epub 2021 Jan 30.

Department of Economic, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

This study aims to determine the moderating role of IT capability between green intellectual capital (GIC) and business sustainability in China's manufacturing sector. A quantitative analysis approach is applied to the primary data collected through a close-ended survey questionnaire from 1000 supply chain employees of China's manufacturing industry. Results revealed that the association and effect of GIC on the business sustainability of Chinese manufacturing companies were statistically significant, while the moderating role of IT capability between GIC and business sustainability was also found. Notably, all variables indicating GIC (i.e., green human capital (GHC), green structural capital (GSC), and green relationship capital (GRC)) affected business sustainability positively and significantly, whereas the moderation of IT capability was specific to GSC and GRC. Hence, it has been recommended to the Chinese manufacturing companies to integrate IT infrastructure in their firms to enhance business sustainability. However, this research was limited to the Chinese manufacturing sector and cannot be generalized to other sectors or countries.
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http://dx.doi.org/10.1007/s11356-020-12245-2DOI Listing
June 2021

ab initio study of oxygen vacancy effects on structural, electronic and thermoelectric behavior of AZrMO (A = Ba, Ca, Sr; M= Al, Cu, x = 0.25) for application of memory devices.

J Mol Graph Model 2021 03 21;103:107825. Epub 2020 Dec 21.

Division of Electronics and Electrical Engineering, Dongguk University, Seoul, 04620, Republic of Korea.

The structural, electronic and thermoelectric properties of AZrMO (A = Ba, Ca, Sr; M = Al, Cu, x = 0.25) without and with an oxygen vacancy (Vo) have been unveiled using the Perdew-Burke-Ernzerhof Generalized Gradient Approximation (PBE-GGA) functional along with Tran-Blaha modified Becke-Jonhson (TB-mBJ)approximation based on Density Functional Theory (DFT) in the framework of WIEN2k code for memristors applications. Moreover, isosurface charge density plots have been calculated by using Vienna ab initio Simulation Package (VASP) simulation code. The analysis of structural parameters reveals that substituting Zr with Al and Cu causes the lattice distortion which tends to increase in the presence of Vo along with dopant. The study of band structure, density of states (DOS) and isosurface charge density plots predict the enhanced charge conduction and formation of conducting filaments (CFs) for all composites with dopant and/or Vo. Moreover, spin polarized density of states for Cu doped composites has also been calculated to confirm the large exchange splitting of Cu-3d states. The thermoelectric characteristics of considered composites have also been explored using the Boltztrap code to better explain the semi-classical Boltzmann transport theory. Thermoelectric parameters confirm the semiconductor nature of all composites, ensuring the compatibility for memristors and thermoelectric devices applications. In addition to this spin polarized thermoelectric behavior of Cu doped composites that ensure the contribution of spin down (↓) states of Cu for charge transport mechanism. The SrZrCuO+Vo composite is found most promising candidate followed by BaZrCuO for memristors applications while, CaZrCuO is found most suitable amongst studied composites for thermoelectric devices.
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http://dx.doi.org/10.1016/j.jmgm.2020.107825DOI Listing
March 2021

Multimodal imaging of skin lesions by using methylene blue as cancer biomarker.

Microsc Res Tech 2020 Dec 14;83(12):1594-1603. Epub 2020 Aug 14.

Biophotonics Imaging Techniques Laboratory, Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

This study aimed the optical imaging of malignant and normal skin tissues with multimodal wide-field fluorescence polarization imaging (WF) technique, by using methylene blue as fluorescence dye. We present optical imaging of skin tissues by different techniques, including reflectance, fluorescence, and polarization imaging for early detection of skin cancer. We collected the reflectance confocal images at 390 and 500 nm. For wide-field fluorescence images, specimens were stimulated at 640 nm and images were collected between 670 and 710 nm. The correlation of the regarded optical modalities with histopathology (H&E), their potentials, capabilities, and limitations to detect skin lesions are discussed. The advantages of multimodal imaging of skin tissues are analyzed to divulge possibilities for precise tumor boundary detection and their classification for malignant and nonmalignant skin tissues. Prior to imaging, the cells were stained in aqueous MB (a dye approved by FDA).
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http://dx.doi.org/10.1002/jemt.23555DOI Listing
December 2020

An overview on radiometric assessment and excess lifetime cancer risk of soil in Pakistan by using High Purity Germanium (HPGe) detector.

Rev Environ Health 2020 Nov 14;35(4):531-543. Epub 2020 Jul 14.

Department of Physics, Islamia University of Bahawalpur, Bahawalpur, Pakistan.

Objectives: The aim of this study is to compare the natural radioactivity and excess life time cancer risk (ELCR) factor of soil in different regions of Pakistan during last decade. Soil contains various elements and compounds including naturally occurring radioactive elements (U, Th, K and Cs). Human being, animals and plants are in health risk by contaminations of natural radioactivity in soil and environmental radiometric pollution. Transferring of large amount of the natural radioactive elements in human body by nutrients may cause carcinogenic effects in human body. Pakistani soil has six types as Indus Basin Soil, Bongar Soil, Khaddar Soil, Indus delta soil, Mountainous soil and Sandy Desert Soil. In some northern region of Pakistan, naturally occurring radioactive rocks like uranuium-238 and iridium concentrations present in Gharwandi, Aram, Kingri S, Vitakri Fort Munro, Dera Bugti, Kohlu and Sibbi districts.

Methods: In this reviewed data, gamma rays spectroscopy used to determine the concentrations of U, Th and K with the help of High Purity Germanium (HPGe) detectors. Only the data of HPGe detector collected because of comparisons of different regions of Pakistan.

Results And Conclusions: Mostly, different gamma rays energy peaks of relevant daughter radionuclides of radioactive element were used such as the energy peak lines of daughter radionuclides Pb (295.21 and 352 KeV) and Bi (609 and 1,120 KeV) used for calculating the Ra concentration in soil. In the recent study, it is concluded that average values of concentrations of natural radioactivity in soil in central and north regions of Pakistan are higher than permissible limit but found permissible range in south region of Pakistan. Mean values of ELCR factor were found higher, equal and lower in central, north and south regions than permissible limit, respectively. Generally, no serious health hazard due to natural radioactivity in soil were found.
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http://dx.doi.org/10.1515/reveh-2019-0094DOI Listing
November 2020

Hematological complications under hyper conditions of glucose and distilled water.

Pak J Pharm Sci 2020 Mar;33(2):589-595

Bio-Photonics Research Laboratory Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

This work presents a pilot method of hematological diagnosis about changes in: shape, size and rouleaux formation, cell count of leucocytes and platelet cells in the presence of different glucose [CHO] and water [H2O] concentrations. The 2D microscopic images after addition of ten different glucose concentrations to normal blood (0 mM- 450 mM) revealed the lyses (disintegration) of white blood cells (WBCs). This work provides a baseline to diagnose blood disorders and complications at labs and clinical environment.
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March 2020

Quality, quantity and hematological disorders in blood under Ethanol analyte an in vitro study.

Pak J Pharm Sci 2020 Mar;33(2):561-566

Bio-photonics Imaging Techniques Laboratory Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

The purpose of this manuscript is to analyze the quantity, quality and disorders of blood components and blood parameters before and after addition of five different concentrations i.e. (0μl, 10μl, 20μl, 30μl and 40μl) of ethyl alcohol (ethanol) in the blood of Brest cancer patient. We diagnose the disorders in cells count, shape of cells, size of cells and many blood parameters like Hemoglobin, Red blood cells distribution width, Mean corpuscular volume, Platelet crit, Mean platelet volume, Platelet distribution width, Hematocrit, Serum glutamic pyruvic transaminase, Cholesterol, Low-density lipoprotein, triglycerides, Serum glutamic oxaloacetic transaminase, high-density lipoprotein, Urea and Total Billirubin by using hematological techniques. We have noted that the number of WBCs and RBCs decrease sharply while platelet cells increases gradually. We also have revealed the shape changes like WBCs show disintegration around RBCs and clumping of Platelet cells. This work will be extended towards the three dimensional imaging using Optical Coherence Tomography (OCT) for monitoring the level of Ethanol in blood via vasculature maps both for the normal and breast cancer.
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March 2020

Sonochemical-driven ultrafast facile synthesis of SnO nanoparticles: Growth mechanism structural electrical and hydrogen gas sensing properties.

Ultrason Sonochem 2017 01 21;34:484-490. Epub 2016 Jun 21.

Center of Excellenece in Nanotechnology (CENT) and Chemistry Department KFUPM, Dhahran 31261, Saudi Arabia; Chemistry Department, KFUPM, Dhahran 31261, Saudi Arabia. Electronic address:

Synthesis of SnO nanoparticles have been successfully accomplished moderately at lower temperature by facile, rapid, efficient and mild ultrasonic irradiation method. The as-grown SnO nanoparticles are investigated by various characterization techniques in terms of structural, optical, electrical and gas sensing properties. XRD investigation has shown that the SnO nanoparticles materials exhibit single rutile crystal phase with high crystallinity. FESEM studies showed uniform and monodisperse morphology of SnO nanoparticles. The chemical composition of SnO was systematically studied by EDX measurements. Additional confirmation of three Raman shifts (432, 630, 772cm) indicated the characteristic properties of the rutile phase of the as-grown SnO nanoparticles. The optical properties of SnO nanoparticles were examined by DRS, and the electronic band gap of SnO nanoparticles were around 3.6eV. Electrical properties of the SnO nanoparticles measured at various temperatures have shown the semiconducting properties. Surface area and pore size of synthesized nanoparticles were analyzed from BET. It has been revealed that SnO nanoparticles have surface area is 47.8574m/g and the pore size is 10.5nm. Moreover, hydrogen gas sensor made of SnO nanoparticles showed good sensitivity and faster response for the hydrogen gas. This method is template-less and surfactant-free which circumvents rigorous reaction work-up for the former removal, reaction temperature and reaction time compared to hydrothermal synthesis and pertinent to many other oxide materials.
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http://dx.doi.org/10.1016/j.ultsonch.2016.06.025DOI Listing
January 2017

Monitoring of glucose, salt and pure water in human whole blood: An in vitro study.

Pak J Pharm Sci 2016 Jul;29(4):1237-42

Material Simulation Research laboratory (MSRL), Department of Physics, Bahauddin Zakariya University, University Campus, Bosan Road, Multan, Pakistan.

Designing and implementation of non-invasive methods for glucose monitoring in blood is main focus of biomedical scientists to provide a relief from skin puncturing of diabete patient. The objective of this research work is to investigate the shape deformations and the aggregation of red blood cells (RBCs) in the human blood after addition of three different analytes i) (0mM-400mM: Range) of glucose (C(6)H(12)O(6)), ii) (0mM-400mM: range) of pure salt (NaCl) and iii) (0mM- 350mM: range) of pure water (H(2)O). We have observed that the changes in the shape of individual cells from biconcave discs to spherical shapes and eventually the lysis of the cells at optimum concentration of glucose, salts and pure water. This demonstration also provides a base line to facilitate diabetes during partial diagnosis and monitoring of the glucose levels qualitatively both in research laboratories and clinical environment.
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July 2016

Lack of association of the NPAS2 gene Ala394Thr polymorphism (rs2305160:G>A) with risk of chronic lymphocytic leukemia.

Asian Pac J Cancer Prev 2014 ;15(17):7169-74

Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan E-mail :

Background: NPAS2 is a product of the circadian clock gene. It acts as a putative tumor suppressor by playing an important role in DNA damage responses, cell cycle control and apoptosis. Chronic lymphocytic leukemia (CLL) appears to be an apoptosis related disorder and alteration in the NPAS2 gene might therefore be directly involved in the etiology of CLL. Here, the Ala394Thr polymorphism (rs2305160:G>A) in the NPAS2 gene was genotyped and melatonin concentrations were measured in a total of seventy-four individuals, including thirty-seven CLL cases and an equal number of age- and sex-matched healthy controls in order to examine the effect of NPAS2 polymorphism and melatonin concentrations on CLL risk in a Pakistani population.

Materials And Methods: Genotyping of rs2305160:G>A polymorphism at NPAS2 locus was carried out by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Melatonin concentrations were determined by enzyme linked immunosorbent assay (ELISA). Statistical analysis was performed using Statistical Package for Social Sciences software.

Results: Our results demonstrated no association of the variant Thr genotypes (Ala/ Thr and Thr/Thr) with risk of CLL. Similarly, no association of rs2305160 with CLL was observed in either females or males after stratification of study population on a gender basis. Moreover, when the subjects with CLL were further stratified into shift-workers and non-shift-workers, no association of rs2305160 with CLL was seen in either case. However, significantly low serum melatonin levels were observed in CLL patients as compared to healthy subjects (p<0.05). Also, lower melatonin levels were seen in shift-workers as compared to non-shift-workers (p<0.05). There was no significant difference (p>0.05) in the melatonin levels across NPAS2 genotypes in all subjects, subjects with CLL who were either shift workers or non-shift-workers. General Linear Model (GLM) univariate analysis revealed no significant association (p>0.05) of the rs2305160 polymorphism of the NPAS2 gene with melatonin levels in any of the groups.

Conclusions: While low melatonin levels and shift-work can be considered as one of the risk factors for CLL, the NPAS2 rs2305160 polymorphism does not appear to have any association with risk of CLL in our Pakistani population.
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http://dx.doi.org/10.7314/apjcp.2014.15.17.7169DOI Listing
June 2015

Deregulated expression of circadian clock and clock-controlled cell cycle genes in chronic lymphocytic leukemia.

Mol Biol Rep 2014 Jan 5;41(1):95-103. Epub 2013 Nov 5.

Department of Physiology & Cell Biology, University of Health Sciences, Lahore, Pakistan.

Circadian rhythms are endogenous and self-sustained oscillations of multiple biological processes with approximately 24-h rhythmicity. Circadian genes and their protein products constitute the molecular components of the circadian oscillator that form positive/negative feedback loops and generate circadian rhythms. The circadian regulation extends from core clock genes to various clock-controlled genes that include various cell cycle genes. Aberrant expression of circadian clock genes, therefore, may lead to genomic instability and accelerated cellular proliferation potentially promoting carcinogenesis. The current study encompasses the investigation of simultaneous expression of four circadian clock genes (Bmal1, Clock, Per1 and Per2) and three clock-controlled cell cycle genes (Myc, Cyclin D1 and Wee1) at mRNA level and determination of serum melatonin levels in peripheral blood samples of 37 CLL (chronic lymphocytic leukemia) patients and equal number of age- and sex-matched healthy controls in order to indicate association between deregulated circadian clock and manifestation of CLL. Results showed significantly down-regulated expression of Bmal1, Per1, Per2 and Wee1 and significantly up-regulated expression of Myc and Cyclin D1 (P < 0.0001) in CLL patients as compared to healthy controls. When expression of these genes was compared between shift-workers and non-shift-workers within the CLL group, the expression was found more aberrant in shift-workers as compared to non-shift-workers. However, this difference was found statistically significant for Myc and Cyclin D1 only (P < 0.05). Serum melatonin levels were found significantly low (P < 0.0001) in CLL subjects as compared to healthy controls whereas melatonin levels were found still lower in shift-workers as compared to non-shift-workers within CLL group (P < 0.01). Our results suggest that aberrant expression of circadian clock genes can lead to aberrant expression of their downstream targets that are involved in cell proliferation and apoptosis and hence may result in manifestation of CLL. Moreover, shift-work and low melatonin levels may also contribute in etiology of CLL by further perturbing of circadian clock.
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http://dx.doi.org/10.1007/s11033-013-2841-7DOI Listing
January 2014

Colorization and automated segmentation of human T2 MR brain images for characterization of soft tissues.

PLoS One 2012 27;7(3):e33616. Epub 2012 Mar 27.

Department of Computer Science and Information Technology, The Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan.

Characterization of tissues like brain by using magnetic resonance (MR) images and colorization of the gray scale image has been reported in the literature, along with the advantages and drawbacks. Here, we present two independent methods; (i) a novel colorization method to underscore the variability in brain MR images, indicative of the underlying physical density of bio tissue, (ii) a segmentation method (both hard and soft segmentation) to characterize gray brain MR images. The segmented images are then transformed into color using the above-mentioned colorization method, yielding promising results for manual tracing. Our color transformation incorporates the voxel classification by matching the luminance of voxels of the source MR image and provided color image by measuring the distance between them. The segmentation method is based on single-phase clustering for 2D and 3D image segmentation with a new auto centroid selection method, which divides the image into three distinct regions (gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) using prior anatomical knowledge). Results have been successfully validated on human T2-weighted (T2) brain MR images. The proposed method can be potentially applied to gray-scale images from other imaging modalities, in bringing out additional diagnostic tissue information contained in the colorized image processing approach as described.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0033616PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313939PMC
November 2012