Publications by authors named "Haeun Lee"

10 Publications

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High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array.

Genomics Inform 2021 Sep 30;19(3):e34. Epub 2021 Sep 30.

Department of Bioconvergence Engineering, Dankook University, Yongin 16890, Korea.

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. The LOAA dPCR is a semiconductor chip-based separation PCR type equipment. The LOAA dPCR includes Micro Electro Mechanical System that can be injected by partitioning the target gene into 56 to 20,000 wells. The amount of target gene per wells is digitized to 0 or 1 as the number of well gradually increases to 20,000 wells because its principle follows Poisson distribution, which allows the LOAA dPCR to perform precise absolute quantification. LOAA determined region of interest first prior to dPCR operation. To exclude invalid wells for the quantification, the LOAA dPCR has applied various filtering methods using brightness, slope, baseline, and noise filters. As the coronavirus disease 2019 has now spread around the world, needs for diagnostic equipment of point of care testing (POCT) are increasing. The LOAA dPCR is expected to be suitable for POCT diagnosis due to its compact size and high accuracy. Here, we describe the quantitative principle of the LOAA dPCR and suggest that it can be applied to various fields.
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http://dx.doi.org/10.5808/gi.21035DOI Listing
September 2021

Discovery of G Protein-Biased Antagonists against 5-HTR.

J Med Chem 2021 Sep 14;64(18):13766-13779. Epub 2021 Sep 14.

Brain Science Institute, Korea Institute of Science and Technology, Seongbuk-gu, Seoul 02792, Republic of Korea.

5-HTR belongs to a family of G protein-coupled receptors and is associated with a variety of physiological processes in the central nervous system via the activation of the neurotransmitter serotonin (5-HT). To develop selective and biased 5-HTR ligands, we designed and synthesized a series of pyrazolyl-diazepanes and pyrazolyl-piperazines , which were evaluated for binding affinities to 5-HTR subtypes and functional selectivity for G protein and β-arrestin signaling pathways of 5-HTR. Among them, 1-(3-(3-chlorophenyl)-1-pyrazol-4-yl)-1,4-diazepane showed the best binding affinity for 5-HTR and selectivity over other 5-HTR subtypes. It was also revealed as a G protein-biased antagonist. The self-grooming behavior test was performed with in vivo with Shank3 transgenic (TG) mice, wherein significantly reduced self-grooming duration time to the level of wild-type mice. The results suggest that 5-HTR could be a potential therapeutic target for treating autism spectrum disorder stereotypy.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01093DOI Listing
September 2021

Role of Savings and Internal Lending Communities (SILCs) in improving household wealth and financial preparedness for birth in rural Zambia.

Health Policy Plan 2021 Sep;36(8):1269-1278

Associate Dean of Global Affairs, Health Behavior and Biological Sciences, University of Michigan, School of Nursing, 400 North Ingalls Street, Ann Arbor, MI 48109-5482, USA.

Savings and Internal Lending Communities (SILCs) are a type of informal microfinance mechanism adapted in many low- and middle-income countries (LMICs) to improve financial resources for poor and rural communities. Although SILCs are often paired with other health and non-health-related interventions, few studies have examined SILCs in the context of maternal health. This study examined the association between SILC participation, household wealth and financial preparedness for birth. The study also examined the association between sex and financial preparedness for birth. A secondary analysis was conducted on individual survey data collected from SILC participants in two rural districts of Zambia between October 2017 and February 2018. A convenience sample of 600 participants (Lundazi: n = 297; Mansa: n = 303) was analysed. Descriptive analyses were run to examine SILC participation and household wealth. Multiple binary logistic regression models were fit to assess the unadjusted and adjusted relationship between (1) SILC participation and household wealth, (2) SILC participation and financial preparedness for birth and (3) sex and financial preparedness for birth. The results show that SILC participation led to an average increase of 7.32 items of the 13 household wealth items. SILC participants who had their most recent childbirth after joining SILCs were more likely to be financially prepared for birth [adjusted odds ratio (AOR): 2.99; 95% confidence interval (95% CI): 1.70-5.26; P < 0.001] than participants who had their most recent childbirth before joining SILCs. Females were more likely to be financially prepared for birth than males if they had their most recent birth before joining an SILC (AOR: 1.79; 95% CI: 1.16-2.66; P < 0.01). SILC participation is shown to increase household wealth and financial preparedness for birth for both men and women. SILCs are a promising intervention that can help poor and rural populations by increasing financial resources and financially preparing parents for birth.
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http://dx.doi.org/10.1093/heapol/czab049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428586PMC
September 2021

Financial Preparedness for Birth Among Rural Zambian Women: Do Antenatal Care Contacts Make a Difference?

Matern Child Health J 2021 Jan 22;25(1):22-26. Epub 2020 Nov 22.

School of Nursing, University of Michigan, 400 North Ingalls St, Ann Arbor, MI, 48109, USA.

Introduction: Financial constraints are one of the biggest barriers for women of low-income countries to receive necessary reproductive health services. Educating women about the importance of saving money has been incorporated as a component of antenatal care (ANC) contacts, but little is known whether ANC contacts influence women's saving.

Methods: A secondary analysis was conducted on data from a cross-sectional household survey study of 1109 women who recently gave birth in two rural districts of Zambia.

Results: Receiving ANC contacts early and often and discussing saving money during ANC were associated with saving money for the mother's birth, but not with saving enough money for the most recent birth.

Discussion: Continued effort is needed to encourage women to attend ANC contacts earlier and more frequently. Additionally, the importance of saving money for birth should be discussed during ANC contacts. Future studies need to explore why women's action in saving does not necessarily lead to saving enough for childbirth.
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http://dx.doi.org/10.1007/s10995-020-03043-wDOI Listing
January 2021

Total Syntheses of Fimsbactin A and B and Their Stereoisomers to Probe the Stereoselectivity of the Fimsbactin Uptake Machinery in .

Org Lett 2020 04 26;22(7):2806-2810. Epub 2020 Mar 26.

Department of Chemistry, Korea University, Seoul 02841, Republic of Korea.

The stereoselective synthesis of fimsbactin A, a siderophore of the human pathogen , was established. Based on this synthetic route, various fimsbactin stereoisomeric analogues were generated and tested for their iron delivery activity for . This investigation revealed that the fimsbactin uptake machinery in this bacterium was indeed highly stereoselective in substrate recognition.
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http://dx.doi.org/10.1021/acs.orglett.0c00790DOI Listing
April 2020

Scoping Review: Valid and Reliable Health Literacy Assessment Tools Used in Low- and Middle-Income Countries.

J Nurs Meas 2020 Mar 16. Epub 2020 Mar 16.

University of Michigan, Ann Arbor, Michigan.

Background And Purpose: Despite the importance of health literacy (HL) in health outcomes, most HL assessment tools are developed and used in first world, English speaking countries, and their applicability in low and middle-income countries (LMICs) is unclear. The purpose of this review is to examine valid and reliable tools used to assess HL among people in LMICs.

Methods: A literature search of three databases was conducted with 20 final articles.

Results: Current HL assessment tools used in LMICs rely on participant's literacy and focus on measuring people's functional HL rather than reflecting the evolving definitions of HL.

Conclusions: More research needs to be conducted to better understand how people with limited literacy acquire and apply HL, and HL assessment tools need to assess various aspects of HL beyond one's functional HL.
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http://dx.doi.org/10.1891/JNM-D-18-00071DOI Listing
March 2020

Semantic Segmentation of White Matter in FDG-PET Using Generative Adversarial Network.

J Digit Imaging 2020 08;33(4):816-825

Department of Medical Engineering, Yonsei University College of Medicine, Seoul, South Korea.

In the diagnosis of neurodegenerative disorders, F-18 fluorodeoxyglucose positron emission tomography/computed tomography (F-FDG PET/CT) is used for its ability to detect functional changes at early stages of disease process. However, anatomical information from another modality (CT or MRI) is still needed to properly interpret and localize the radiotracer uptake due to its low spatial resolution. Lack of structural information limits segmentation and accurate quantification of the F-FDG PET/CT. The correct segmentation of the brain compartment in F-FDG PET/CT will enable the quantitative analysis of the F-FDG PET/CT scan alone. In this paper, we propose a method to segment white matter in F-FDG PET/CT images using generative adversarial network (GAN). The segmentation result of GAN model was evaluated using evaluation parameters such as dice, AUC-PR, precision, and recall. It was also compared with other deep learning methods. As a result, the proposed method achieves superior segmentation accuracy and reliability compared with other deep learning methods.
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http://dx.doi.org/10.1007/s10278-020-00321-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522152PMC
August 2020

Synthesis and Characterization of Anguibactin To Reveal Its Competence To Function as a Thermally Stable Surrogate Siderophore for a Gram-Negative Pathogen, Acinetobacter baumannii.

Org Lett 2018 10 8;20(20):6476-6479. Epub 2018 Oct 8.

Department of Chemistry , Korea University , 145 Anam-ro, Seongbuk-gu , Seoul 02841 , Republic of Korea.

Total synthesis of anguibactin was accomplished for the first time, and the following biochemical characterizations allowed for the determination of its Fe(III) binding mode as well as the demonstration of its iron delivery capability for Acinetobacter baumannii. These properties, in addition to the thermal stability over acinetobactin, render anguibactin as a competent surrogate siderophore that can be useful for the future development of a siderophore-based antibiotic delivery system against A. baumannii.
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http://dx.doi.org/10.1021/acs.orglett.8b02789DOI Listing
October 2018

Preconception Health Behaviors of Low-Income Women.

MCN Am J Matern Child Nurs 2016 Sep-Oct;41(5):293-8

Adejoke B. Ayoola is Robert Wood Johnson Foundation Nurse Faculty Scholar, Associate Professor, Department of Nursing, Calvin College, Grand Rapids, MI. The author can be reached via e-mail at Krista Sneller is a Registered Nurse Consultant, City of Seattle, Seattle, WA. Tega D. Ebeye is a Baccalaureate nursing undergraduate student, Calvin College, Grand Rapids, MI. Megan Jongekrijg Dykstra is a Baccalaureate nursing undergraduate student, Calvin College, Grand Rapids, MI. Victoria L. Ellens is a Registered Nurse, Holland Hospital, Holland, MI. HaEun Grace Lee is a Baccalaureate nursing undergraduate student, Calvin College, Grand Rapids, MI. Gail L. Zandee is an Associate Professor, Department of Nursing, Calvin College, Grand Rapids, MI.

Purpose: Preconception behaviors have a significant impact on birth outcomes, particularly among low-income minority groups, and women with unplanned pregnancies. This study examined women's perceived health status and behaviors such as drinking, smoking, exercise, and use of multivitamins and folic acid.

Study Design And Methods: This was a descriptive study based on a convenience sample of women living in urban underserved neighborhoods. Univariate and bivariate analyses were conducted using STATA 13.

Results: The sample consisted of 123 women ages 18 to 51 years (mean = 30.57); 51.22% were Hispanic, 36.59% African American, and 12.2% Caucasian. Over 70% had a household income of less than $20,000, 57.72% had no health insurance in the last year, and 58.54% were not married. These women were below the Healthy People 2020 goals for drinking, smoking, and multivitamin use, especially those who were planning to get pregnant in the next 6 months or not sure of their pregnancy planning status. There were no significant differences on any of the preconception health behavior variables based on pregnancy intention.

Clinical Implications: Nurses and healthcare providers should emphasize importance of practicing healthy behaviors during the preconception period among low-income ethnic minority women specifically those living in urban medically underserved areas who are unsure of their pregnancy planning status or are at risk of unintended pregnancy.
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http://dx.doi.org/10.1097/NMC.0000000000000263DOI Listing
March 2018
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