Publications by authors named "Gustavo Souza"

220 Publications

Stress memory of physiological, biochemical and metabolomic responses in two different rice genotypes under drought stress: The scale matters.

Plant Sci 2021 Oct 17;311:110994. Epub 2021 Jul 17.

Department of Botany, Institute of Biology - Section of Plant Physiology, Federal University of Pelotas, Pelotas, RS, Brazil.

Drought is a pivotal cause for crop yield reductions. When subjected to recurrent external stimuli, plants can develop memory of stress responses that, eventually, enables improved plant tolerance to environmental changes. In addition, despite causal relationships, these responses may vary according to hierarchical levels of observation. Thus, this study aims to check the responses of recurrent and non-recurrent stresses in two rice genotypes observing their drought memory responses at different levels of organization, that is, on a physiological, biochemical and metabolomic scale and for end in global PCA. For this, seventy variables were measured on the scales described in order to obtain a large number of observations. The memory responses were evident in almost all scales observed. The lowland genotype, especially plants not subjected to recurrent water shortage, showed higher damage to the photosynthetic apparatus under drought conditions, although it has exhibited more evident memory response effect after rehydration. On the other hand, the upland genotype appears to be more tolerant to drought insofar lower biochemical damage was observed. Specific behaviors of each genotype at biochemical and metabolomics levels and similar behavior at physiological level were observed. This study demonstrates the importance of observation at different hierarchical levels.
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http://dx.doi.org/10.1016/j.plantsci.2021.110994DOI Listing
October 2021

Plastome evolution in the Caesalpinia group (Leguminosae) and its application in phylogenomics and populations genetics.

Planta 2021 Jul 8;254(2):27. Epub 2021 Jul 8.

Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, Brazil.

Main Conclusion: The chloroplast genomes of Caesalpinia group species are structurally conserved, but sequence level variation is useful for both phylogenomic and population genetic analyses. Variation in chloroplast genomes (plastomes) has been an important source of information in plant biology. The Caesalpinia group has been used as a model in studies correlating ecological and genomic variables, yet its intergeneric and infrageneric relationships are not fully solved, despite densely sampled phylogenies including nuclear and plastid loci by Sanger sequencing. Here, we present the de novo assembly and characterization of plastomes from 13 species from the Caesalpinia group belonging to eight genera. A comparative analysis was carried out with 13 other plastomes previously available, totalizing 26 plastomes and representing 15 of the 26 known Caesalpinia group genera. All plastomes showed a conserved quadripartite structure and gene repertoire, except for the loss of four ndh genes in Erythrostemon gilliesii. Thirty polymorphic regions were identified for inter- or intrageneric analyses. The 26 aligned plastomes were used for phylogenetic reconstruction, revealing a well-resolved topology, and dividing the Caesalpinia group into two fully supported clades. Sixteen microsatellite (cpSSR) loci were selected from Cenostigma microphyllum for primer development and at least two were cross-amplified in different Leguminosae subfamilies by in vitro or in silico approaches. Four loci were used to assess the genetic diversity of C. microphyllum in the Brazilian Caatinga. Our results demonstrate the structural conservation of plastomes in the Caesalpinia group, offering insights into its systematics and evolution, and provides new genomic tools for future phylogenetic, population genetics, and phylogeographic studies.
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http://dx.doi.org/10.1007/s00425-021-03655-8DOI Listing
July 2021

Belowground interactions affect shoot growth in under restrictive conditions.

Plant Signal Behav 2021 Sep 30;16(9):1927589. Epub 2021 May 30.

Laboratory of Plant Cognition and Electrophysiology (LACEV), Department of Botany, Institute of Biology, Federal University of Pelotas, Pelotas, RS, Brazil.

Plant-plant interactions like competition or facilitation between seedlings can have profound implications on their establishment and posterior development. These interactions are variable and depend upon the presence of neighbouring plants and environmental factors. In this work, we studied the effects of the interaction by the roots of seedlings from a population under various environmental stressful conditions: water deficit, nutrient deficit, low light, low temperature, and high temperature. To evaluate it, we measured some growth and morphological parameters. We demonstrated that shoot parameters such as the number of leaves, leaf area, and dry weight of the leaves were the most affected parameters due to the belowground plant-plant interaction under various environmental conditions. We did not find evidence for competition among the plants, especially under restrictive conditions. Therefore, the study corroborates the stress-gradient hypothesis, which states that plants' differences under stressful conditions lead to facilitative interactions. It has implications for plant ecology and forestry techniques.
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http://dx.doi.org/10.1080/15592324.2021.1927589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280888PMC
September 2021

Aiming off the target: recycling target capture sequencing reads for investigating repetitive DNA.

Ann Bot 2021 May 29. Epub 2021 May 29.

Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife-PE, Brazil.

Background And Aims: With the advance of high-throughput sequencing (HTS), reduced-representation methods such as target capture sequencing (TCS) emerged as cost-efficient ways of gathering genomic information, particularly from coding regions. As the off-target reads from such sequencing are expected to be similar to genome skimming (GS), we assessed the quality of repeat characterization in plant genomes using this data.

Methods: Repeat composition obtained from TCS datasets of five Rhynchospora (Cyperaceae) species were compared with GS data from the same taxa. In addition, a FISH probe was designed based on the most abundant satellite found in the TCS dataset of Rhynchospora cephalotes. Finally, repeat-based phylogenies of the five Rhynchospora species were constructed based on the GS and TCS dataset and the topologies were compared with a gene-alignment based phylogenetic tree.

Key Results: All the major repetitive DNA families were identified in TCS, including repeats that showed abundances as low as 0.01% in the GS data. Rank correlation between GS and TCS repeat abundances were moderately high (r = 0.58-0.85), increasing after filtering out the targeted loci from the raw TCS reads (r = 0.66-0.92). Repeat data obtained by TCS was also reliable to develop a cytogenetic probe of a new variant of the holocentromeric satellite Tyba. Repeat-based phylogenies from TCS data were congruent with those obtained from GS data and the gene-alignment tree.

Conclusions: Our results show that off-target TCS reads can be recycled to identify repeats for cyto- and phylogenomic investigations. Given the growing availability of TCS reads, driven by global phylogenomic projects, our strategy represents a way to recycle genomic data and contribute to a better characterization of plant biodiversity.
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http://dx.doi.org/10.1093/aob/mcab063DOI Listing
May 2021

Heat shock during in vitro maturation of bovine oocytes disturbs bta-miR-19b and DROSHA transcripts abundance after in vitro fertilization.

Reprod Domest Anim 2021 Aug 8;56(8):1128-1136. Epub 2021 Jun 8.

Reproduction and Biotechnology Laboratory, Brazilian Agricultural Research Corporation (Embrapa), Juiz de Fora, Minas Gerais, Brazil.

While microRNAs (miRNAs) are a class of non-coding RNAs important for embryo development, the relationship between them and heat stress during oocyte maturation has not yet been established. This study investigated the effect of heat shock during in vitro maturation (IVM) on the abundance of bta-miR-20a, -27b, -103, -21-5p, -19b, -1246 miRNAs and DROSHA and DICER1 mRNAs, previously reported for being involved in oocyte maturation, response to heat stress and miRNA biogenesis. Oocytes were exposed for 12h to heat shock during IVM, fertilized in vitro and the presumptive zygotes cultured for eight days. The relative quantification of miRNAs and mRNAs was performed by real-time PCR in vitro-matured oocytes and 8-cell stage embryos. Progression of meiosis, embryonic development and apoptotic indices was also evaluated. Heat shock compromised (p < .05) oocyte nuclear maturation, cleavage and embryo development, with a higher (p < .05) embryonic apoptotic index than the control group. The abundance of bta-miR-19b increased (p < .05) whereas the abundance of DROSHA transcripts decreased (p < .05) in embryos derived from heat-shocked oocytes. In conclusion, heat shock during IVM influences the abundance of bta-miR-19b and DROSHA in pre-implantation embryos, indicating a persistent effect of heat shock that can be associated with impaired embryo development.
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http://dx.doi.org/10.1111/rda.13956DOI Listing
August 2021

Fractal dimension analysis as an easy computational approach to improve breast cancer histopathological diagnosis.

Appl Microsc 2021 Apr 30;51(1). Epub 2021 Apr 30.

Department of Biology - Genetics - Federal University of Juiz de Fora, Rua José Lourenço Kelmer, s/n, Juiz de Fora, Minas Gerais, 36036-900, Brazil.

Histopathology is a well-established standard diagnosis employed for the majority of malignancies, including breast cancer. Nevertheless, despite training and standardization, it is considered operator-dependent and errors are still a concern. Fractal dimension analysis is a computational image processing technique that allows assessing the degree of complexity in patterns. We aimed here at providing a robust and easily attainable method for introducing computer-assisted techniques to histopathology laboratories. Slides from two databases were used: A) Breast Cancer Histopathological; and B) Grand Challenge on Breast Cancer Histology. Set A contained 2480 images from 24 patients with benign alterations, and 5429 images from 58 patients with breast cancer. Set B comprised 100 images of each type: normal tissue, benign alterations, in situ carcinoma, and invasive carcinoma. All images were analyzed with the FracLac algorithm in the ImageJ computational environment to yield the box count fractal dimension (Db) results. Images on set A on 40x magnification were statistically different (p = 0.0003), whereas images on 400x did not present differences in their means. On set B, the mean Db values presented promissing statistical differences when comparing. Normal and/or benign images to in situ and/or invasive carcinoma (all p < 0.0001). Interestingly, there was no difference when comparing normal tissue to benign alterations. These data corroborate with previous work in which fractal analysis allowed differentiating malignancies. Computer-aided diagnosis algorithms may beneficiate from using Db data; specific Db cut-off values may yield ~ 99% specificity in diagnosing breast cancer. Furthermore, the fact that it allows assessing tissue complexity, this tool may be used to understand the progression of the histological alterations in cancer.
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http://dx.doi.org/10.1186/s42649-021-00055-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087740PMC
April 2021

Brazilian essential oils as source for the discovery of new anti-COVID-19 drug: a review guided by in silico study.

Phytochem Rev 2021 Apr 13:1-20. Epub 2021 Apr 13.

Laboratório de Fitotecnologia, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in China and its spread worldwide has become one of the biggest health problem due to the lack of knowledge about an effective chemotherapy. Based on the current reality of the SARS-CoV-2 pandemic, this study aimed to make a review literature about potential anti-coronavirus natural compounds guided by an in silico study. In the first step, essential oils from native species found in the Brazilian herbal medicine market and Brazilian species that have already shown antiviral potential were used as source for the literature search and compounds selection. Among these compounds, 184 showed high antiviral potential against rhinovirus or picornavirus by quantitative structure-activity relationship analysis. ()-α-atlantone; 14-hydroxy-α-muurolene; allo-aromadendrene epoxide; amorpha-4,9-dien-2-ol; aristochene; azulenol; germacrene A; guaia-6,9-diene; hedycaryol; humulene epoxide II; α-amorphene; α-cadinene; α-calacorene and α-muurolene showed by a molecular docking study the best result for four target proteins that are essential for SARS-CoV-2 lifecycle. In addition, other parameters obtained for the selected compounds indicated low toxicity and showed good probability to achieve cell permeability and be used as a drug. These results guided the second literature search which included other species in addition to native Brazilian plants. The majority presence of any of these compounds was reported for essential oils from 45 species. In view of the few studies relating essential oils and antiviral activity, this review is important for future assays against the new coronavirus.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11101-021-09754-4.
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http://dx.doi.org/10.1007/s11101-021-09754-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042356PMC
April 2021

Factors associated with stress, anxiety, and depression during social distancing in Brazil.

Rev Saude Publica 2021 9;55. Epub 2021 Apr 9.

Universidade Católica de Pernambuco. Centro de Ciências Biológicas e da Saúde. Recife, Pernambuco, PE, Brasil.

Objective: To estimate the prevalence of clinical signs and symptoms of severe/extreme stress, anxiety, and depression, as well as their associated factors, among Brazilians during social distancing.

Methods: This is a cross-sectional study conducted in April/May 2020 with 3,200 Brazilians over 18 years old. Respondents' sociodemographic and clinical data were collected using an online questionnaire, which also included the 21-item Depression, Anxiety and Stress Scale (DASS-21) to assess emotional symptoms. Unadjusted and adjusted prevalence ratios and their respective 95% confidence intervals were estimated using Poisson regression models with robust variance.

Results: Our results show the prevalence of severe/extreme stress was 21.5%, anxiety 19.4%, and depression 21.5%. In the final model, sociodemographic, clinical, and Covid-19-related factors were associated with severe/extreme stress, anxiety, and depression in Brazilians during social distancing due to the Covid-19 pandemic. We found the main factors associated with severe/extreme depression to be young women, brown, single, not religious, sedentary, presenting reduced leisure activities, history of anxiety and depression, increased medication use, and Covid-19 symptoms.

Conclusion: This study may help develop and systematically plan measures aimed to prevent, early identify, and properly manage clinical signs and symptoms of stress, anxiety, and depression during the Covid-19 pandemic.
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http://dx.doi.org/10.11606/s1518-8787.2021055003152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011840PMC
April 2021

Understanding photosynthesis in a spatial-temporal multiscale: The need for a systemic view.

Theor Exp Plant Physiol 2021 Apr 4:1-12. Epub 2021 Apr 4.

LABPLANT, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Av. Humberto Monte SN, Campus do Pici, Bl. 907, Fortaleza, CE CEP 60451-970 Brazil.

In October 2020, at the peak of the COVID-19 pandemic, a group of young Brazilian photosynthesis researchers organized the 1st Brazilian Symposium on Photosynthesis. The event was free and online, with the presence of important guest speakers from all over the world, who discussed their recent works on topics related to the future and perspectives of photosynthesis research. Summarizing the expectations of this symposium we highlighted the importance of adopting a systemic perspective for a better understanding of photosynthesis as a complex and dynamic process. Plants are modular and self-regulating presenting metabolic redundancy and functional degeneration. Among the various biological processes, photosynthesis plays a crucial role in promoting the direct conversion of light energy into carbon skeletons for support growth and productivity. In the past decades, significant advances have been made in photosynthesis at the biophysical, biochemical, and molecular levels. However, this myriad of knowledge has been insufficient to answer crucial questions, such as: how can we understand and eventually increase photosynthetic efficiency and yield in crops subjected to adverse environment related to climate-changing? We believe that a crucial limitation to the whole comprehension of photosynthesis is associated with a vastly widespread classic reductionist view. Moreover, this perspective is commonly accompanied by non-integrative, simplistic, and descriptive approaches to investigate a complex and dynamic process as photosynthesis. Herein, we propose the use of new approaches, mostly based on the Systems Theory, which certainly comes closer to the real world, such as the complex systems that the plants represent.
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http://dx.doi.org/10.1007/s40626-021-00199-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019523PMC
April 2021

Detection of Different Hosts From a Distance Alters the Behaviour and Bioelectrical Activity of .

Front Plant Sci 2021 18;12:594195. Epub 2021 Mar 18.

Laboratory of Plant Cognition and Electrophysiology, Department of Botany, Institute of Biology, Federal University of Pelotas, Pelotas, Brazil.

In our study, we investigated some physiological and ecological aspects of the life of Mart. (Convolvulaceae) plants with the hypothesis that they recognise different hosts at a distance from them, and they change their survival strategy depending on what they detect. We also hypothesised that, as an attempt of prolonging their survival through photosynthesis, the synthesis of chlorophylls (a phenomenon not completely explained in these parasitic plants) would be increased if the plants don't detect a host. We quantified the pigments related to photosynthesis in different treatments and employed techniques such as electrophysiological time series recording, analyses of the complexity of the obtained signals, and machine learning classification to test our hypotheses. The results demonstrate that the absence of a host increases the amounts of chlorophyll , chlorophyll , and β-carotene in these plants, and the content varied depending on the host presented. Besides, the electrical signalling of dodders changes according to the species of host perceived in patterns detectable by machine learning techniques, suggesting that they recognise from a distance different host species. Our results indicate that electrical signalling might underpin important processes such as foraging in plants. Finally, we found evidence for a likely process of attention in the dodders toward the host plants. This is probably to be the first empirical evidence for attention in plants and has important implications on plant cognition studies.
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http://dx.doi.org/10.3389/fpls.2021.594195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012508PMC
March 2021

In silico approach of secondary metabolites from Brazilian herbal medicines to search for potential drugs against SARS-CoV-2.

Phytother Res 2021 Aug 1;35(8):4297-4308. Epub 2021 Apr 1.

Laboratório de Fitotecnologia, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

The new severe acute respiratory syndrome coronavirus (SARS-CoV-2) recently emerged as a worrying pandemic, with many confirmed cases and deaths globally. Therefore, there is a clear need for identifying effective therapeutic options and a review of secondary metabolites related to Brazilian herbal medicines was performed as a strategy for the discovery of new antiviral agents. To confirm this potential, an in silico screening of the identified compounds identified was also evaluated. The review was performed by the PubMed database and the selected natural compounds were subjected to in silico analysis such as QSAR, molecular docking and ADMET. 497 secondary metabolites were identified from 23 species. The in silico assays indicated 19 potential anti-SARS-CoV-2 compounds, being triterpenes and phenolic compounds. The indicated compounds showed a high affinity with proteins considered as the main molecular targets against SARS-CoV-2 and parameters indicated low toxicity. In addition to Brazilian medicinal plants, these compounds can be found in other species and they can be a base for the synthesis of other anti-COVID-19 drugs. Therefore, this review is important to conduct researches that address the emerging need for drugs in COVID-19 treatment.
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http://dx.doi.org/10.1002/ptr.7097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250981PMC
August 2021

Proposal of an index of stability for evaluating plant drought memory: A case study in sugarcane.

J Plant Physiol 2021 May 3;260:153397. Epub 2021 Mar 3.

Laboratory of Plant Physiology 'Coaracy M. Franco', Center for Research and Development in Ecophysiology and Biophysics, Agronomic Institute (IAC), Campinas, SP, Brazil.

Stability is a key trait for plant growth and development in a changing environment, involving homeostasis and resilience. While homeostasis refers to the maintenance of the internal structural and functional plant integrity, resilience is associated with the plant ability in returning to the initial conditions after a given disturbance. Such concepts are especially relevant for perennial and semi-perennial plants facing seasonal and frequent stress conditions. Although plant memory is closely associated with plant performance under recurrent stresses, to date, there is no study evaluating how stress memory is linked to stability under varying water conditions. Herein, we evaluated the association between drought stability and memory in sugarcane plants and proposed a new stability index to evaluate plant memory. Two datasets were analyzed, the first deals with leaf gas exchange and photochemistry of sugarcane plants grown in nutrient solution and exposed to one, two or three water deficit cycles. The second takes into account the physiological performance of sugarcane propagules obtained by vegetative propagation from plants that faced drought. To quantify sugarcane stability, we estimated the drought impact, the disturbance rate (DR), drought perturbation, and recovery rate (RR) for plants from both datasets. Drought memory - given by improved performance after previous stress events or when origin material faced drought - was detected in both datasets, changing either DR or RR. Based on these indices, we proposed the overall stability (OSt), defined as the ratio between RR and DR. While DR is associated to plant homeostasis, RR is a measure of plant resilience. Sugarcane plants exposed to three cycles of water deficit or those propagules originated from stressed plants presented the highest OSt values, showing higher RR and/or lower DR when compared to well-watered plants or to propagules from well-watered plants. Regarding the physiological traits evaluated, leaf CO assimilation and stomatal conductance were the most consistent variables in revealing drought stability and memory. Concluding, OSt revealed consistently patterns of response associated with plant memory, besides quantifying plant stability under stressful conditions.
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http://dx.doi.org/10.1016/j.jplph.2021.153397DOI Listing
May 2021

Human Blood Plasma Investigation Employing 2D UPLC-UDMS Data-Independent Acquisition Proteomics.

Methods Mol Biol 2021 ;2259:153-165

Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.

Proteomic tools are especially useful when it comes to investigating complex samples such as human blood plasma, in which protein quantities can span across up to ten orders of magnitude. Ultra definition mass spectrometry, in combination with two-dimensional liquid chromatography, provides better coverage of complex proteomes and allows for better control of collision energy, keeping the fragmentation benefits of high collision energy associated with drift time measurements from ion mobility separation. Here, we present a protocol to assist in the identification of proteins in human blood plasma and other similar samples with a large dynamic range.
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http://dx.doi.org/10.1007/978-1-0716-1178-4_9DOI Listing
April 2021

Proteomic analyses identify major vault protein as a prognostic biomarker for fatal prostate cancer.

Carcinogenesis 2021 May;42(5):685-693

Department of Clinical Medicine, The Arctic University of Norway, Tromsø, Norway.

The demographic shift toward an older population will increase the number of prostate cancer cases. A challenge in the treatment of prostate cancer is to avoid undertreatment of patients at high risk of progression following curative treatment. These men can benefit from early salvage treatment. An explorative cohort consisting of tissue from 16 patients who underwent radical prostatectomy, and were either alive or had died from prostate cancer within 10 years postsurgery, was analyzed by mass spectrometry analysis. Following proteomic and bioinformatic analyses, major vault protein (MVP) was identified as a putative prognostic biomarker. A publicly available tissue proteomics dataset and a retrospective cohort of 368 prostate cancer patients were used for validation. The prognostic value of the MVP was verified by scoring immunohistochemical staining of a tissue microarray. High level of MVP was associated with more than 4-fold higher risk for death from prostate cancer (hazard ratio = 4.41, 95% confidence interval: 1.45-13.38; P = 0.009) in a Cox proportional hazard models, adjusted for Cancer of the Prostate Risk Assessments Post-surgical (CAPRA-S) score and perineural invasion. Decision curve analyses suggested an improved standardized net benefit, ranging from 0.06 to 0.18, of adding MVP onto CAPRA-S score. This observation was confirmed by receiver operator characteristics curve analyses for the CAPRA-S score versus CAPRA-S and MVP score (area under the curve: 0.58 versus 0.73). From these analyses, one can infer that MVP levels in combination with CAPRA-S score might add onto established risk parameters to identify patients with lethal prostate cancer.
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http://dx.doi.org/10.1093/carcin/bgab015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163044PMC
May 2021

Tri- and dipeptides identification in whey protein and porcine liver protein hydrolysates by fast LC-MS/MS neutral loss screening and de novo sequencing.

J Mass Spectrom 2021 Feb;56(2):e4701

Campus Avançado Jandaia do Sul, Universidade Federal do Paraná (UFPR), 86900-000, Jandaia do Sul, Brazil.

We describe a fast (5 min) liquid chromatography tandem mass spectrometry method (LC-MS/MS) based on a 46 Da neutral loss of formic acid (H O and CO) to identify tri- and dipeptides (DIPEP) in whey protein and porcine liver protein hydrolysates and confirmed by further de novo sequencing. Sample solutions were acidified to favor [dipep + H] ions, and a m/z range of 50-300 was used to improve sensitivity. All dipeptide candidates were selected based on all possibilities of the 20 amino acid combinations, and their collision-induced dissociation fragments were screened via de novo sequencing. To determine their biological activities, sequenced dipeptides were compared with the Biopep database and other data from literature. Altogether, 18 dipeptides and 7 tripeptides were identified from the whey protein hydrolysate; they seemed to be broadly active, and peptides were identified as active dipeptidyl peptidase IV inhibitors and active angiotensin-converting enzyme (ACE), according to available information. Porcine liver hydrolysate showed 14 dipeptides which exhibit similar biological activities to whey protein hydrolysate.
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http://dx.doi.org/10.1002/jms.4701DOI Listing
February 2021

Investigation of a new oxazolidine derivative in human resistance acute leukemia cells: deciphering its mechanism of action by label-free proteomic.

Naunyn Schmiedebergs Arch Pharmacol 2021 06 21;394(6):1153-1166. Epub 2021 Jan 21.

Research Center for Therapeutic Innovation (NUPIT-SG), Federal University of Pernambuco, Professor Moraes Rêgo s/n, Cidade Universitária, Recife, Pernambuco, 50670-901, Brazil.

The present study aimed to evaluate the mechanism of action of the antineoplastic activity of an oxazolidine derivative, LPSF/NB-3 (5-(4-cloro-benzilideno)-3-etil-2-tioxo-oxazolidin-4-ona). Cytotoxicity assays were performed in peripheral blood mononuclear cells (PBMCs) and resistant acute leukemia cell line (HL-60/MX1) by the MTT method. LPSF/NB-3 exhibited cytotoxicity in HL-60/MX1, but it was not toxic to healthy cells in the highest dose tested (100 μM). The protein extract of HL-60/MX1 cells treated with LPSF/NB-3 was subjected to proteomic analysis using two-dimensional chromatography coupled to mass spectrometry. We could identify a total of 2652 proteins, in which 633 were statistically modulated. Within the group of protein considered for the quantitative analysis with the established criteria, 262 were differentially expressed, 146 with increased expression and 116 with decreased expression in the sample treated with LPSF/NB-3 compared to the control. The following differentially expressed pathways were found: involving regulation of the cytoskeleton, DNA damage, and transduce cellular signals. Networks that were highlighted are related to the immune system. The ELISA technique was used to assess the immunomodulatory potential of LPSF/NB-3 in PBMCs. We observed significant decrease of IFNγ (p < 0.01) and dose-response pattern of the cytokines IL-6, IL-17A, IL-22, and IL-10. Therefore, results suggest that LPSF/NB-3 appears to modulate important pathways, including cell cycle and immune system regulatory pathways.
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http://dx.doi.org/10.1007/s00210-020-02024-8DOI Listing
June 2021

Pregnant women infected by the Zika virus: Ultrasound findings and growth patterns of fetuses with and without microcephaly.

Int J Gynaecol Obstet 2021 Sep 12;154(3):474-480. Epub 2021 Feb 12.

Biological and Health Sciences Center, Catholic University of Pernambuco (UNICAP), Recife, Brazil.

Objective: To compare ultrasound growth measurements of fetuses with and without microcephaly in suspected Zika virus infection.

Methods: A retrospective cohort study included pregnant women with suspected Zika virus infection to evaluate 110 fetuses with and without microcephaly. The women had been admitted to the fetal medicine unit between October 2015 and August 2016. Cases of fetal microcephaly resulting from other causes were excluded. Variables evaluated were the ultrasound measurements taken at fetal biometry. The relation between each fetal biometry measurement and gestational age was analyzed using fractional polynomials in random-effects regression models. To evaluate fetal growth, curves of the mean fetal biometric parameters were constructed as a function of gestational age.

Results: Mean biparietal diameter and mean head circumference increased in both groups as a function of gestational age. In the group with fetal microcephaly, mean head circumference was significantly larger in the 13th and 14th weeks of pregnancy, becoming smaller compared with the group without microcephaly from the 20th week onwards, with the difference increasing with gestational age.

Conclusion: Fetal head circumference continues to increase until birth, even after a diagnosis of microcephaly, with a reduction only in the pace of growth. Growth decelerates as the pregnancy approaches term.
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http://dx.doi.org/10.1002/ijgo.13578DOI Listing
September 2021

Preparation of glass-ionomer cement containing ethanolic Brazilian pepper extract (Schinus terebinthifolius Raddi) fruits: chemical and biological assays.

Sci Rep 2020 12 18;10(1):22312. Epub 2020 Dec 18.

Departamento de Química, Universidade Federal de Ouro Preto, UFOP, Ouro Preto, 35400-000, Brazil.

Plants may contain beneficial or potentially dangerous substances to humans. This study aimed to prepare and evaluate a new drug delivery system based on a glass-ionomer-Brazilian pepper extract composite, to check for its activity against pathogenic microorganisms of the oral cavity, along with its in vitro biocompatibility. The ethanolic Brazilian pepper extract (BPE), the glass-ionomer cement (GIC) and the composite GIC-BPE were characterized by scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and thermal analysis. The BPE compounds were identified by UPLC-QTOF-MS/MS. The release profile of flavonoids and the mechanical properties of the GIC-BPE composite were assessed. The flavonoids were released through a linear mechanism governing the diffusion for the first 48 h, as evidenced by the M/M relatively to [Formula: see text], at a diffusion coefficient of 1.406 × 10cm s. The ATR-FTIR analysis indicated that a chemical bond between the GIC and BPE components may have occurred, but the compressive strength of GIC-BPE does not differ significantly from that of this glass-ionomer. The GIC-BPE sample revealed an ample bacterial activity at non-cytotoxic concentrations for the human fibroblast MRC-5 cells. These results suggest that the prepared composite may represent an alternative agent for endodontic treatment.
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http://dx.doi.org/10.1038/s41598-020-79257-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749175PMC
December 2020

Effects of resveratrol on the proliferation and osteogenic differentiation of deciduous dental pulp stem cells from neurofibromatosis type 1 patient.

Childs Nerv Syst 2021 04 20;37(4):1095-1101. Epub 2020 Nov 20.

Basic Life Sciences Department, Federal University of Juiz de Fora, Governador Valadares, 35020-670, Brazil.

Purpose: We aimed at verifying whether resveratrol can decrease cell proliferation and change osteogenic differentiation of cells obtained from patients with type 1 neurofibromatosis (NF1).

Methods: Deciduous dental pulp derived stem cells were isolated from NF1 patient and healthy volunteer. These cells were subjected to increasing concentrations of resveratrol and evaluated for proliferation and mineralization of osteogenic differentiation.

Results: The results showed that resveratrol reduced the difference in proliferation between CNT and NF1 cells in a dose-dependent manner and this property was more prominent in affected cells than in healthy cells. Resveratrol showed no statistically significant changes in mineralization in osteogenic differentiation of NF1 cells, at low doses tested.

Conclusions: In conclusion, in a dose-dependent manner, resveratrol displays interesting properties that could be applied in a possible treatment aimed at decreasing cellular proliferation in neurofibromatosis. Furthermore, it is selective concerning healthy cells and not affecting cell differentiation. Further research to cell selectivity, differentiation to other tissue types, and cell cytotoxicity are needed.
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http://dx.doi.org/10.1007/s00381-020-04968-xDOI Listing
April 2021

The Protein-Protein Interaction Network Reveals a Novel Role of the Signal Transduction Protein PII in the Control of c-di-GMP Homeostasis in Azospirillum brasilense.

mSystems 2020 Nov 3;5(6). Epub 2020 Nov 3.

Departamento de Bioquímica e Biologia Molecular, UFPR Curitiba, Curitiba, PR, Brazil

The PII family comprises a group of widely distributed signal transduction proteins ubiquitous in prokaryotes and in the chloroplasts of plants. PII proteins sense the levels of key metabolites ATP, ADP, and 2-oxoglutarate, which affect the PII protein structure and thereby the ability of PII to interact with a range of target proteins. Here, we performed multiple ligand fishing assays with the PII protein orthologue GlnZ from the plant growth-promoting nitrogen-fixing bacterium to identify 37 proteins that are likely to be part of the PII protein-protein interaction network. Among the PII targets identified were enzymes related to nitrogen and fatty acid metabolism, signaling, coenzyme synthesis, RNA catabolism, and transcription. Direct binary PII-target complex was confirmed for 15 protein complexes using pulldown assays with recombinant proteins. Untargeted metabolome analysis showed that PII is required for proper homeostasis of important metabolites. Two enzymes involved in c-di-GMP metabolism were among the identified PII targets. A PII-deficient strain showed reduced c-di-GMP levels and altered aerotaxis and flocculation behavior. These data support that PII acts as a major metabolic hub controlling important enzymes and the homeostasis of key metabolites such as c-di-GMP in response to the prevailing nutritional status. The PII proteins sense and integrate important metabolic signals which reflect the cellular nutrition and energy status. Such extraordinary ability was capitalized by nature in such a way that the various PII proteins regulate different facets of metabolism by controlling the activity of a range of target proteins by protein-protein interactions. Here, we determined the PII protein interaction network in the plant growth-promoting nitrogen-fixing bacterium The interactome data along with metabolome analysis suggest that PII functions as a master metabolic regulator hub. We provide evidence that PII proteins act to regulate c-di-GMP levels and cell motility and adherence behaviors.
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http://dx.doi.org/10.1128/mSystems.00817-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646526PMC
November 2020

How diverse is heterochromatin in the Caesalpinia group? Cytogenomic characterization of Erythrostemon hughesii Gagnon & G.P. Lewis (Leguminosae: Caesalpinioideae).

Planta 2020 Sep 12;252(4):49. Epub 2020 Sep 12.

Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Rua Nelson Chaves S/N, Cidade Universitaria, Recife, PE, 50670-420, Brazil.

Main Conclusion: Cytogenomic characterization of Erythrostemon hughesii reveals a heterogeneity of repeats in its subtelomeric heterochromatin. Comparative analyses with other Caesalpinia group species reveal a significant reduction in the abundance of Ty3-gypsy/Chromovirus Tekay retrotransposons during its evolution. In numerically stable karyotypes, repetitive DNA variability is one of the main causes of genome and chromosome variation and evolution. Species from the Caesalpinia group (Leguminosae) are karyotypically characterized by 2n = 24, with small chromosomes and highly variable CMA heterochromatin banding patterns that correlate with environmental variables. Erythrostemon hughesii differs from other species of the group examined to date for having subtelomeric CMA bands; this contrasts with most species in the group which have proximal bands. Here we analyse the repeatome of E. hughesii using genome skimming and chromosomal mapping approaches to characterize the identity of the most abundant repetitive elements and their physical location. The repetitive fraction of E. hughesii comprises 28.73% of the genome. The most abundant elements were retrotransposons (RT) with long terminal repeats (LTR-RT; 9.76%) and satellite DNAs (7.83%). Within the LTR-RTs, the most abundant lineages were: Ty1/copia-Ale (1%), Ty3/gypsy CRM (0.88%) and Ty3/gypsy Athila (0.75%). Using fluorescent in situ hybridization four satellite DNAs and several LTR-RT elements were shown to be present in most subtelomeric CMA bands. These results highlight how the repeatome in E. hughesii, a species from Oaxaca state in Mexico, is clearly distinct from Northeast Brazilian species of the Caesalpinia group, mainly due to its high diversity of repeats in its subtelomeric heterochromatic bands and low amount of LTR-RT Ty3/gypsy-Tekay elements. Comparative sequence analysis of Tekay elements from different species is congruent with a clade-specific origin of this LTR-RT after the divergence of the Caesalpinia group. We hypothesize that repeat-rich heterochromatin may play a role in leading to faster genomic divergence between individuals, increasing speciation and diversification.
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http://dx.doi.org/10.1007/s00425-020-03453-8DOI Listing
September 2020

Salt-dependent hypertension and inflammation: targeting the gut-brain axis and the immune system with Brazilian green propolis.

Inflammopharmacology 2020 Oct 12;28(5):1163-1182. Epub 2020 Aug 12.

Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Morro Do Cruzeiro, Ouro Preto, MG, 35400-000, Brazil.

Systemic arterial hypertension (SAH) is a major health problem around the world and its development has been associated with exceeding salt consumption by the modern society. The mechanisms by which salt consumption increase blood pressure (BP) involve several homeostatic systems but many details have not yet been fully elucidated. Evidences accumulated over the last 60 decades raised the involvement of the immune system in the hypertension development and opened a range of possibilities for new therapeutic targets. Green propolis is a promising natural product with potent anti-inflammatory properties acting on specific targets, most of them participating in the gut-brain axis of the sodium-dependent hypertension. New anti-hypertensive products reinforce the therapeutic arsenal improving the corollary of choices, especially in those cases where patients are resistant or refractory to conventional therapy. This review sought to bring the newest advances in the field articulating evidences that show a cross-talking between inflammation and the central mechanisms involved with the sodium-dependent hypertension as well as the stablished actions of green propolis and some of its biologically active compounds on the immune cells and cytokines that would be involved with its anti-hypertensive properties.
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http://dx.doi.org/10.1007/s10787-020-00742-2DOI Listing
October 2020

pharmacological prediction and cytotoxicity of flavonoids glycosides identified by UPLC-DAD-ESI-MS/MS in extracts of leaves cultivated in Brazil.

Nat Prod Res 2020 Aug 7:1-6. Epub 2020 Aug 7.

Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.

Ethanolic (EB) extract and hexanic (SH) and hydromethanolic (SEM) sub-extracts of leaves were submitted to cytotoxicity evaluation and to phytochemical methods. The effect of EB and SEM on cellular cycle was evaluated by propidium iodide method and the phases were quantified through flow cytometry. The cytotoxicity assessment was done using T24 and MRC5 cells, with EB and SEM (25-1200 µg/mL). By means of UPLC-DAD-MS/MS data were identified the flavonoids astragaline, nicotiflorin, kaempferol-7--rutinoside, robinin, hyperin, rutin, quercetin-7--rutinoside and manghaslin. EB (800 µg/mL) and SEM (1200 µg/mL) reduces the T24 cell viability. These extracts at 25 µg/mL stimulate the growth of MRC5 cells, evidencing a selective cytotoxicity. After 24 h of the treatment with extracts was not observed cycle arrest of T24 cells. The bioactivity prediction of the flavonoids was evaluated through Active-IT software and PASSonline which indicated potential activity as antitumoral, cytotoxic, anti-inflammatory, antiparasitic, antimicrobial, antiviral and others.
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http://dx.doi.org/10.1080/14786419.2020.1803308DOI Listing
August 2020

Melaleuca leucadendra Essential Oil Promotes Loss of Cell Membrane and Wall Integrity and Inhibits Bacterial Growth: An In Silico and In Vitro Approach.

Curr Microbiol 2020 Sep 22;77(9):2181-2191. Epub 2020 May 22.

Department of Pharmacy, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.

Essential oils are potential antimicrobial agents and can be used as active ingredients in the pharmaceutical, food and cosmetics industries. This work intends to evaluate the antibacterial activity and design a strategy for the proposition of the mechanism of action of Melaleuca leucadendra essential oil. Optimum concentration of the bacteria and the phase where they had the highest pathogenic activity were determined. Results show that for each microorganism it is necessary to use a different concentration at the time of adjusting the initial inoculum, and that the time to achieve exponential growth phase varies from one to the other. M. leucadendra essential oil demonstrated in vitro antimicrobial properties. This oil was chemically characterized and the main compounds were evaluated by their mechanism of antibacterial action based on structure-activity analysis. The mechanism is related to the increase of bacteria cell membrane permeability. This indication was confirmed by flow cytometry and transmission electronic microscopy. Thus, in silico analysis is an important tool in the search for new antimicrobial agents and these results showed that M. leucadendra essential oil may be useful on the development of new chemotherapies or food preservation systems.
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http://dx.doi.org/10.1007/s00284-020-02024-0DOI Listing
September 2020

EpitoCore: Mining Conserved Epitope Vaccine Candidates in the Core Proteome of Multiple Bacteria Strains.

Front Immunol 2020 5;11:816. Epub 2020 May 5.

Bioinformatics Multidisciplinary Environment, Universidade Federal do Rio Grande Do Norte-UFRN, Natal, Brazil.

In reverse vaccinology approaches, complete proteomes of bacteria are submitted to multiple computational prediction steps in order to filter proteins that are possible vaccine candidates. Most available tools perform such analysis only in a single strain, or a very limited number of strains. But the vast amount of genomic data had shown that most bacteria contain pangenomes, i.e., their genomic information contains core, conserved genes, and random accessory genes specific to each strain. Therefore, in reverse vaccinology methods it is of the utmost importance to define core proteins and core epitopes. EpitoCore is a decision-tree pipeline developed to fulfill that need. It provides surfaceome prediction of proteins from related strains, defines core proteins within those, calculate their immunogenicity, predicts epitopes for a given set of MHC alleles defined by the user, and then reports if epitopes are located extracellularly and if they are conserved among the core homologs. Pipeline performance is illustrated by mining peptide vaccine candidates in strains. From a total proteome of ~4,800 proteins per strain, EpitoCore predicted 103 highly immunogenic core homologs located at cell surface, many of those related to virulence and drug resistance. Conserved epitopes identified among these homologs allows the users to define sets of peptides with potential to immunize the largest coverage of tested HLA alleles using peptide-based vaccines. Therefore, EpitoCore is able to provide automated identification of conserved epitopes in bacterial pangenomic datasets.
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http://dx.doi.org/10.3389/fimmu.2020.00816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214623PMC
March 2021

Quantitative Proteomic Analysis of MARC-145 Cells Infected with a Mexican Porcine Reproductive and Respiratory Syndrome Virus Strain Using a Label-Free Based DIA approach.

J Am Soc Mass Spectrom 2020 Jun 21;31(6):1302-1312. Epub 2020 May 21.

Departamento de Genética y Biologı́a Molecular, Cinvestav-IPN, Ciudad de México, C.P. 07360, México.

Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease characterized by severe reproductive failure in sows, acute respiratory disorders in growing pigs, and high mortality in piglets. The causative agent of this syndrome is the PRRS virus (PRRSV), an RNA virus belonging to the Arteriviridae family. To date, several quantitative approaches of proteomics have been applied to analyze the gene expression profiles during PRRSV infection in PAMs and MARC-145 cells, and few proteins have been consistent among independent studies, probably due to the differences in the levels of virulence of different PRRSV strains used and/or due to analytical conditions. In this study, total proteins isolated from noninfected and infected MARC-145 cells with a Mexican PRRSV strain were relatively quantified using label-free based DIA approach in combination with ion-mobility separation. As a result, 1456 quantified proteins were found to be shared between the control and infected samples. Afterward, these proteins were filtered, and 699 of them were considered without change. Also, 17 proteins were up-regulated and 19 proteins were down-regulated during the PRSSV infection. Bioinformatic analysis revealed that many of the differentially expressed proteins are involved in processes like antigen processing, presentation of antigens, response to viruses, response to IFNs, and innate immune response, among others. The present work is the first one which provides a detailed proteomic analysis through label-free based DIA approach in MARC-145 cells during the infection with a Mexican PRRSV strain.
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http://dx.doi.org/10.1021/jasms.0c00134DOI Listing
June 2020

Proteome alterations associated with the oleic acid and cis-9, trans-11 conjugated linoleic acid content in bovine skeletal muscle.

J Proteomics 2020 06 23;222:103792. Epub 2020 Apr 23.

"Luiz de Queiroz" College of Agriculture (ESALQ), University of São Paulo (USP), Piracicaba, SP 13418-900, Brazil. Electronic address:

Oleic acid (OA) and cis-9, trans-11 conjugated linoleic acid (c9t11-CLA) are fatty acids found in beef with beneficial effects in human health. This study investigated differentially abundant proteins (DAPs) in skeletal muscle of bovines with extreme values of OA, and c9t11-CLA. For each one of the fatty acids, twenty muscle samples were divided into two groups (N = 10_High; N = 10_Low) and analyzed by high definition mass spectrometry. We identified 103 and 133 DAPs between the groups for each fatty acid. We found 64 and 45 up-regulated and 39 and 68 down-regulated proteins for OA and c9t11-CLA, respectively. Comparative analysis between proteomic and transcriptomic data revealed eight and ten genes with a consistent between mRNA expression levels and protein abundance for OA and c9t11-CLA, respectively. Unconventional myosin-Id (MYO1D), mineralocorticoid receptor (NR3C2), geranylgeranyl transferase type-2 subunit-alpha (RABGGTA), and uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA) were found as putative candidate proteins for OA content. Fatty acid synthase (FASN), tubulin alpha-4A chain (TUBA4A), vinculin (VCL), NADH dehydrogenase 1 alpha subcomplex 5 (NDUFA5), and prefoldin subunit 6 (PFDN6) for c9t11-CLA. Our findings contribute to a deeper understanding of the molecular mechanisms behind the regulation of the OA and c9t11-CLA content in cattle skeletal muscle. SIGNIFICANCE: Questions about the association between meat intake and disease incidence in humans has driven animal scientist to pursue a better understanding of the biological processes associated with differences in the intramuscular fat composition. The beneficial effects of oleic acid and conjugated linoleic acid in human health have been demonstrated by improving the immune system and preventing atherosclerosis, different types of cancers, hypertension, and diabetes. Previous genome-wide association and gene expression studies identified genomic regions and differentially expressed genes associated with the fatty acid profile in skeletal muscle. In this work, differences were evaluated at the protein level. The use of a label-free quantitative proteomic approach, compared with muscle transcriptome results obtained by RNA-sequencing, allowed us to earn new insights into the variability in fatty acid deposition in skeletal muscle of farm animals. This study opens new avenues to explore the effect of the fatty acids in the skeletal muscle of livestock animals, which is associated with nutritional values of the meat, and perhaps to understand the mechanisms correlated with metabolic diseases in other species.
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http://dx.doi.org/10.1016/j.jprot.2020.103792DOI Listing
June 2020

Antimicrobial peptide selection from Lippia spp leaf transcriptomes.

Peptides 2020 07 22;129:170317. Epub 2020 Apr 22.

Genetics and Biotechnology Graduate Program, Juiz de Fora Federal University, Juiz de Fora, Brazil. Electronic address:

Antimicrobial resistance is considered a health issue worldwide. This public health problem underscores the importance of searching for new antimicrobial molecules with different mechanisms of action. Leaf transcriptomes were used to search and develop synthetic antimicrobial peptides derived from mRNA sequences. The in silico search for new AMPs from the L. rotundifolia and L. alba transcriptomes allowed the identification of 120 putative peptide mRNA sequences. Eight of them fitted into optimal parameters and were translated and chemically synthesized antimicrobial peptides. Their biological activity was tested in both Gram-positive and Gram-negative bacteria against which they exhibited antibacterial activity. However, they showed an important hemolytic effect. Afterwards, two active peptides showing bactericidal activity isolated from each plant transcriptome tested were modified and modeled in 11 new variants to increase their antimicrobial activity and stability and to reduce or eliminate their hemolytic effect from their original peptides. The La-AMP1 (MSLLERKLLMHFLRV) the original peptide from L. alba showed a 52% hemolytic effect while the derived peptide La-AMP1a (GLMKLLRELLHMFSRVG) had its hemolytic effect reduced to 0.5% at 128 μg.mL. Similarly, we observed that the original peptide from L. rotundifolia, Lr-AMP1 (MRIGLRFVLM), displayed a 71.5% hemolytic effect, while its derived peptide Lr-AMP1f (GSVLRAIMRMFAKLMG) showed 0% hemolysis at 128 μg.mL, tested with fresh human erythrocytes. Our results indicate a promising method for the search for novel antimicrobial agents with reduced or zero hemolytic effect, as well as prediction and optimization of their activity from plant mRNA libraries.
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http://dx.doi.org/10.1016/j.peptides.2020.170317DOI Listing
July 2020

Divide to Conquer: Evolutionary History of Allioideae Tribes (Amaryllidaceae) Is Linked to Distinct Trends of Karyotype Evolution.

Front Plant Sci 2020 7;11:320. Epub 2020 Apr 7.

Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, Brazil.

Allioideae (e.g., chives, garlics, onions) comprises three mainly temperate tribes: Allieae (800 species from the northern hemisphere), Gilliesieae (80 South American species), and Tulbaghieae (26 Southern African species). We reconstructed the phylogeny of Allioideae (190 species plus 257 species from Agapanthoideae and Amaryllidoideae) based on ITS, K, F, and L to investigate its historical biogeography and karyotype evolution using newly generated cytomolecular data for Chilean Gilliesieae genera , , , and . The crown group of Allioideae diversified ∼62 Mya supporting a Gondwanic origin for the subfamily and vicariance as the cause of the intercontinental disjunction of the tribes. Our results support the hypothesis of the Indian tectonic plate carrying Allieae to northern hemisphere ('out-of-India' hypothesis). The colonization of the northern hemisphere (∼30 Mya) is correlated with a higher diversification rate in associated to stable = 8, increase of polyploidy and the geographic expansion in Europe and North America. Tulbaghieae presented = 6, but with numerical stability (2 = 12). In contrast, the tribe Gilliesieae ( = 6) varied considerably in genome size (associated with Robertsonian translocations), rDNA sites distribution and chromosome number. Our data indicate that evolutionary history of Allioideae tribes is linked to distinct trends of karyotype evolution.
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http://dx.doi.org/10.3389/fpls.2020.00320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155398PMC
April 2020

The Correlation between Hand Grip Strength and Nutritional Variables in Ambulatory Cancer Patients.

Nutr Cancer 2021 14;73(2):221-229. Epub 2020 Apr 14.

Program of Master in Science, Technology and Food Safety, University Center of Maringá, Maringá, Paraná, Brazil.

Among the methods used in the nutritional assessment of cancer patients, handgrip strength (HGS), an important indicator of malnutrition, was chosen in this study. An observational cross-sectional study with the prospective collection. We analyzed 100 patients from the Oncology Department of Santa Rita Hospital, in Maringá city, Paraná, Brazil. To determine the clinical-pathological staging was used the Cancer Staging Manual. In the objective nutritional assessment, data to calculate Body Mass Index (BMI), arm muscle circumference (AMC), corrected arm muscle area (cAMA) and measurement of HGS were measured. In connection with the subjective method used, a Patient-Generated Subjective Global Assessment (PG-SGA) has been selected. Arm Circumference (AC) presents a significant association with the left hand HGS classification. The right hand HGS does not show a significant correlation with the analyzed variables. However, the results showed that patients with higher left hand HGS tend to present lower PG-SGA and higher AMC. HGS, as a predictor of nutritional vulnerability, has proved superiority for predicting clinical results in cancer patients. However, it requires more studies that investigate other nutritional variables to determine the nutritional risk in this population.
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http://dx.doi.org/10.1080/01635581.2020.1750662DOI Listing
August 2021
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