Publications by authors named "Guozhen Chen"

30 Publications

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[Exploring the relationship of family life, resilience and bullying with the use of tobacco in preadolescents].

Wei Sheng Yan Jiu 2021 Sep;50(5):763-774

School of Public Health and Management, Youjiang Nationality Medical College, Baise 533000, China.

Objective: The study aimed at association of family life, resilience and bullying on the use of tobacco in preadolescents.

Methods: A total of 4792 students from 5 junior schools in Baise City were recruited with cluster-sampling method, filled with questionnaire of family life, resilience, parents' Control, bullying, initiation of tobacco and smoking from Feb. to Nov. 2018.The sample comprised of 52.63% male students and 46.66% female students. The average age was(11.8±0.5). There were 56.78% of students lived in city and 43.22% of students lived in county town; The nationality of the sample was as follows: Zhuang nationality 90.00%, Han nationality 7.62%, other minorities(Yao nationality, Miao nationality, Yi nationality, et al)2.05%. The Logistic regression was used to explore the effect.

Results: There were 9.75% and 6.97% of the sample reported initiation of tobacco and smoking respectively. The initiation of tobacco and smoking of boys were higher than that of girls(initiation of tobacco: χ~2=57.230, P<0.001; smoking: χ~2=56.013, P<0.001). The multivariate Logistic analysis showed gender was statistically significant factor of initiation of tobacco(OR=0.468, 95%CI 0.377-0.582) and smoking(OR=0.422, 95% CI 0.324-0.551), and age was statistically significant factor of initiation of tobacco(OR=1.609, 95% CI 1.446-1.791) and smoking(OR=2.026, 95%CI 1.776-2.310). Bullying was statistically significant factors of smoking(OR=1.106, 95% CI 1.073-1.140). Three protective factors were associated with a lower likelihood of initiation of tobacco(individual power: OR=0.964, 95% CI 0.951-0.976; family cohesion, OR=0.946, 95% CI 0.892-0.984; family rules, OR=0.949, 95%CI 0.930-0.965) and smoking(individual power: OR=0.962, 95% CI 0.947-0.977; family cohesion, OR=0.937, 95%CI 0.885-0.992; family rules, OR=0.952, 95%CI 0.932-0.973)in the final subscale model.

Conclusion: Bullying increased the risk of smoking, while Individual power, family cohesion and family rules were associated with a lower likelihood of initiation of tobacco and smoking in preadolescents.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.05.010DOI Listing
September 2021

Fluorescence and electrochemical assay for bimodal detection of lead ions based on Metal-Organic framework nanosheets.

Talanta 2021 Sep 19;232:122405. Epub 2021 Apr 19.

College of Chemistry & Materials Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi, 710069, China. Electronic address:

The accurate measurement of heavy metal ions is essential for human health and environmental protection. Here, we report the design of a simple and convenient bimodal strategy for signal-on, label-free lead ion detection in environmental samples based on two-dimensional metal-organic framework (2D-MOF) nanosheets. 2D-MOFs have different affinities toward guanine-rich DNA (ssGDNA) and the G-quadruplex, allowing these structures to be distinguished. The nanosheets were also used as quenchers for fluorescent lead ion detection. Using lead ions to induce G-quadruplex formation from ssGDNA, a simple fluorescence resonance energy transfer (FRET) strategy was developed for lead ion detection; the detection limit was 3.3 nM. Based on changes in the GDNA configuration, the FRET system was converted into an electrochemical sensor for lead ion assays using an electrode modified with the 2D-MOF nanosheets. Electrochemical impedance spectroscopy showed a high sensitivity and a low limit of detection (i.e., 8.7 pM) of the electrode. The adaptability of the bimodal mechanism was verified through the successful detection of lead ions in tap water and fertilizer samples, and the method accuracy was demonstrated through inductively coupled plasma analysis. The developed bimodal device is cost-effective, highly sensitive, and allows for convenient operation, thereby rendering it a promising and reliable system for the detection of lead ions in environmental samples.
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http://dx.doi.org/10.1016/j.talanta.2021.122405DOI Listing
September 2021

Endothelial Cell-Derived SO Controls Endothelial Cell Inflammation, Smooth Muscle Cell Proliferation, and Collagen Synthesis to Inhibit Hypoxic Pulmonary Vascular Remodelling.

Oxid Med Cell Longev 2021 17;2021:5577634. Epub 2021 Apr 17.

Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.

Hypoxic pulmonary vascular remodelling (PVR) is the major pathological basis of aging-related chronic obstructive pulmonary disease and obstructive sleep apnea syndrome. The pulmonary artery endothelial cell (PAEC) inflammation, and pulmonary artery smooth muscle cell (PASMC) proliferation, hypertrophy and collagen remodelling are the important pathophysiological components of PVR. Endogenous sulfur dioxide (SO) was found to be a novel gasotransmitter in the cardiovascular system with its unique biological properties. The study was aimed to investigate the role of endothelial cell- (EC-) derived SO in the progression of PAEC inflammation, PASMC proliferation, hypertrophy and collagen remodelling in PVR and the possible mechanisms. EC-specific aspartic aminotransferase 1 transgenic (EC-AAT1-Tg) mice were constructed . Pulmonary hypertension was induced by hypoxia. Right heart catheterization and echocardiography were used to detect mouse hemodynamic changes. Pathologic analysis was performed in the pulmonary arteries. High-performance liquid chromatography was employed to detect the SO content. Human PAECs (HPAECs) with lentiviruses containing AAT1 cDNA or shRNA and cocultured human PASMCs (HPASMCs) were applied . SO probe and enzyme-linked immunosorbent assay were used to detect the SO content and determine p50 activity, respectively. Hypoxia caused a significant reduction in SO content in the mouse lung and HPAECs and increases in right ventricular systolic pressure, pulmonary artery wall thickness, muscularization, and the expression of PAEC ICAM-1 and MCP-1 and of PASMC Ki-67, collagen I, and -SMA ( < 0.05). However, EC-AAT1-Tg with sufficient SO content prevented the above increases induced by hypoxia ( < 0.05). Mechanistically, EC-derived SO deficiency promoted HPAEC ICAM-1 and MCP-1 and the cocultured HPASMC Ki-67 and collagen I expression, which was abolished by andrographolide, an inhibitor of p50 ( < 0.05). Meanwhile, EC-derived SO deficiency increased the expression of cocultured HPASMC -SMA ( < 0.05). Taken together, these findings revealed that EC-derived SO inhibited p50 activation to control PAEC inflammation in an autocrine manner and PASMC proliferation, hypertrophy, and collagen synthesis in a paracrine manner, thereby inhibiting hypoxic PVR.
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http://dx.doi.org/10.1155/2021/5577634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068783PMC
May 2021

Circular RNA THBS1 promotes proliferation and apoptosis of non-small cell lung cancer cells by sponging miR-129-5p and regulating SOX4 expression.

J BUON 2020 Jul-Aug;25(4):1721-1727

Department of Respiration, the PLA Navy Anqing Hospital, Anqing, China.

Purpose: To investigate the influence of circular ribonucleic acid thrombospondin-1 (circTHBS1) on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells by sponging miR-129-5p and regulating the expression of SRY-box transcription factor 4 (SOX4).

Methods: Carcinoma and para-carcinoma specimens were collected from 40 NSCLC patients, and 25 pairs of specimens were obtained from patients with metastatic and non-metastatic NSCLC. After NSCLC cells were cultured, the proliferation was detected via cell counting kit-8 (CCK-8) and 5-Ethynyl-2'- deoxyuridine (EdU) assays, and the cell cycle and apoptosis rate were analyzed through flow cytometry. Finally, the action targets of circTHBS1 were determined using dual-luciferase reporter gene assay, and Western blotting assay was applied to measure the changes in protein levels.

Results: The expression of circTHBS1 was markedly higher in NSCLC patients than that in control group, and it was increased in patients with metastatic NSCLC compared with that in patients with non-metastatic NSCLC. Moreover, the proliferative ability of the cells was weakened notably after transfection with small interfering (Si)-CircTHBS1, but it was enhanced remarkably after transfection with CircTHBS1-overexpression vector (OE). There were complementary sites in circTHBS1 for the 3'-UTR of miR-129-5p, and the fluorescence intensity of wild-type circTHBS1 declined evidently after interacting with miR-129-5p. Besides, there was a putative binding site between miR-129-5p and SOX4, and SOX4 expression was decreased obviously after overexpressing miR-129-5p but increased following overexpression of circTHBS1.

Conclusions: CircTHBS1 promotes the proliferation and inhibits the apoptosis of NSCLC cells through targeting miR-129-5p and regulating SOX4 expression.
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July 2021

AMD-GAN: Attention encoder and multi-branch structure based generative adversarial networks for fundus disease detection from scanning laser ophthalmoscopy images.

Neural Netw 2020 Dec 17;132:477-490. Epub 2020 Sep 17.

National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China. Electronic address:

The scanning laser ophthalmoscopy (SLO) has become an important tool for the determination of peripheral retinal pathology, in recent years. However, the collected SLO images are easily interfered by the eyelash and frame of the devices, which heavily affect the key feature extraction of the images. To address this, we propose a generative adversarial network called AMD-GAN based on the attention encoder (AE) and multi-branch (MB) structure for fundus disease detection from SLO images. Specifically, the designed generator consists of two parts: the AE and generation flow network, where the real SLO images are encoded by the AE module to extract features and the generation flow network to handle the random Gaussian noise by a series of residual block with up-sampling (RU) operations to generate fake images with the same size as the real ones, where the AE is also used to mine features for generator. For discriminator, a ResNet network using MB is devised by copying the stage 3 and stage 4 structures of the ResNet-34 model to extract deep features. Furthermore, the depth-wise asymmetric dilated convolution is leveraged to extract local high-level contextual features and accelerate the training process. Besides, the last layer of discriminator is modified to build the classifier to detect the diseased and normal SLO images. In addition, the prior knowledge of experts is utilized to improve the detection results. Experimental results on the two local SLO datasets demonstrate that our proposed method is promising in detecting the diseased and normal SLO images with the experts labeling.
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http://dx.doi.org/10.1016/j.neunet.2020.09.005DOI Listing
December 2020

Postural Tachycardia Syndrome in Children and Adolescents: Pathophysiology and Clinical Management.

Front Pediatr 2020 20;8:474. Epub 2020 Aug 20.

Department of Pediatrics, Peking University First Hospital, Beijing, China.

Postural tachycardia syndrome (POTS), characterized by chronic (≥6 months) orthostatic intolerance symptoms with a sustained and excessive heart rate increase while standing without postural hypotension, is common in children and adolescents. Despite the unclear pathogenesis of POTS, the present opinion is that POTS is a heterogeneous and multifactorial disorder that includes altered central blood volume, abnormal autonomic reflexes, "hyperadrenergic" status, damaged skeletal muscle pump activity, abnormal local vascular tension and vasoactive factor release, mast cell activation, iron insufficiency, and autoimmune dysfunction. A number of pediatric POTS patients are affected by more than one of these pathophysiological mechanisms. Therefore, individualized treatment strategies are initiated in the management of POTS, including basal non-pharmacological approaches (e.g., health education, the avoidance of triggers, exercise, or supplementation with water and salt) and special pharmacological therapies (e.g., oral rehydration salts, midodrine hydrochloride, and metoprolol). As such, the recent progress in the pathogenesis, management strategies, and therapeutic response predictors of pediatric POTS are reviewed here.
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http://dx.doi.org/10.3389/fped.2020.00474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468430PMC
August 2020

Amplified Electrochemical Hydrogen Peroxide Sensing Based on Cu-Porphyrin Metal-Organic Framework Nanofilm and G-Quadruplex-Hemin DNAzyme.

ACS Appl Mater Interfaces 2020 Dec 18;12(52):58105-58112. Epub 2020 Dec 18.

College of Chemistry & Materials Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi 710069, China.

A novel electrochemical hydrogen peroxide (HO) sensor based on Cu-porphyrin(Cu-TCPP)/G-quadruplex-hemin nanocomposite was constructed by assembling two-dimensional Cu-TCPP metal-organic framework (MOF) nanofilm and G-quadruplex-hemin DNAzyme. The Cu-TCPP synthesized by the surfactant-assisted method has a wrinkled two-dimensional nanofilm morphology, which gives it a large surface area and accessible active sites. Cu-TCPP exhibits peroxidase activity and good stability and can catalyze the reduction of HO. In addition, Cu-TCPP can be used as a nanocarrier for G-quadruplex-hemin DNAzyme with strong peroxidase activity to achieve "biological barcode" amplification and improve stability. The cooperative interaction of Cu-TCPP and G-quadruplex-hemin DNAzyme effectively amplifies the electrochemical response signal. Electrochemical studies have shown that the constructed sensor exhibits good electrochemical sensing performance with three linear ranges: 0.08 μM to 0.11 mM, 0.11-0.91 mM, and 0.91-8.1 mM, with sensitivities of 2315.86, 301.00, and 65.71 μA/(mM cm), respectively, and the detection limit was 0.03 μM. In addition, the sensor shows good selectivity. In summary, this study provides a simple and effective new strategy for electrochemical sensing based on two-dimensional MOFs and artificial enzymes.
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http://dx.doi.org/10.1021/acsami.0c09254DOI Listing
December 2020

Race- and sex-specific association between alcohol consumption and hypertension in 22 cohort studies: A systematic review and meta-analysis.

Nutr Metab Cardiovasc Dis 2020 07 27;30(8):1249-1259. Epub 2020 Mar 27.

Department of Preventive Medicine, Shenzhen University Health Sciences Center, Shenzhen, Guangdong, People's Republic of China; The Third Affiliated Hospital of Shenzhen University Health Sciences Center, Shenzhen, Guangdong, People's Republic of China; Guangdong Key Laboratory for Genome Stability & Disease Prevention, Shenzhen University Health Science Center, Shenzhen, Guangdong, People's Republic of China. Electronic address:

Background And Aims: The alcohol-hypertension relation has been well documented, but whether women have protective effect or race and type of beverage consumed affect the association remain unclear. To quantify the relation between total or beverage-specific alcohol consumption and incident hypertension by considering the effect of sex and race.

Methods And Results: Articles were identified in PubMed and Embase databases with no restriction on publication date. Pooled relative risks (RRs) and 95% confidence intervals (CIs) were calculated by random effects models. Restricted cubic splines were used to model the dose-response association. This study involved 22 articles (31 studies) and included 414,477 participants. The hypertension risk was different among liquor, wine, and beer at 5.1-10 g/d of ethanol consumption (P = 0.002). The hypertension risk differed between men (RR: 1.14, 95% CI: 1.07, 1.20) and women (RR: 0.98, 95% CI: 0.89, 1.06) at 10 g/d (P = 0.005). We found a linear alcohol-hypertension association among white (P- = 0.017), black people (P- = 0.035), and Asians (P-<0.001). With 10 g/d increment of consumption, the RRs for hypertension were 1.06 (95% CI: 1.04, 1.08), 1.14 (95% CI: 1.01, 1.28), and 1.06 (95% CI: 1.01, 1.10) for Asians, black, and white people, respectively.

Conclusion: Sex modifies the alcohol-hypertension association at low level of alcohol consumption and we did not find evidence of a protective effect of alcohol consumption among women. Black people may have higher hypertension risk than Asians and white people at the same ethanol consumption.
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http://dx.doi.org/10.1016/j.numecd.2020.03.018DOI Listing
July 2020

Qiliqiangxin improves cardiac function and attenuates cardiac remodelling in doxorubicin-induced heart failure rats.

Pharm Biol 2020 Dec;58(1):417-426

School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, P. R. China.

Therapeutic doxorubicin administration is restricted as this anticancer drug may be cardiotoxic. The traditional Chinese medicine qiliqiangxin has been approved for clinical treatment of chronic heart failure. To explore the protective effects and molecular mechanisms of qiliqiangxin on doxorubicin-induced congestive heart failure (CHF) in rats. A CHF rat model was established via intraperitoneal DOX injections (2.5 mg/kg/week) for 6 weeks. The rats were randomly assigned to control, CHF, CHF + QL (1.0 g/kg/d), or captopril (3.8 mg/kg/d) treatment groups ( = 10) for 4 weeks. MicroRNA sequencing elucidated the molecular mechanisms of qiliqiangxin on doxorubicin-induced CHF in rats. Unlike in the CHF group, QL significantly reduced Bax:Bcl-2 (2.05 ± 0.23 vs. 0.94 ± 0.09,  < 0.05) and the levels of collagen I (0.19 ± 0.02 vs. 0.15 ± 0.01,  < 0.05), collagen III (0.19 ± 0.02 vs. 0.14 ± 0.02,  < 0.05), TGF-β1 (5.28 ± 0.89 vs. 2.47 ± 0.51,  < 0.05), Smad3 (1.23 ± 0.12 vs. 0.78 ± 0.09,  < 0.05), MMP-2 (0.89 ± 0.01 vs. 0.53 ± 0.05,  < 0.05), and TIMP-2 (0.24 ± 0.03 vs. 0.44 ± 0.03,  < 0.05). QL also upregulated TGF-β3 (0.65 ± 0.06 vs. 0.96 ± 0.10,  < 0.05) and Smad7 (0.09 ± 0.01 vs. 0.19 ± 0.023,  < 0.05). Moreover, Smad3 was a target of miR-345-3p. The beneficial effects of QL on DOX-induced CHF in rats are mediated by reduction in myocardial fibrosis, promotion of TGF-β3/Smad7, and inhibition of TGF-β1/Smad3. QL may also modulate specific miRNAs. These results provide evidence that QL might be an effective treatment for DOX-induced CHF.
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http://dx.doi.org/10.1080/13880209.2020.1761403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301709PMC
December 2020

Reduced 24-h Sodium Excretion Is Associated With a Disturbed Plasma Acylcarnitine Profile in Vasovagal Syncope Children: A Pilot Study.

Front Pediatr 2020 11;8:98. Epub 2020 Mar 11.

Department of Pediatrics, Peking University First Hospital, Beijing, China.

To investigate if the low sodium intake is associated with the plasma carnitine and acylcarnitine profile in children with vasovagal syncope (VVS). Twenty-six children suffering from VVS were recruited in the present study and divided into a group of low urinary sodium excretion or a group of normal urinary sodium excretion according to the excretion of 24-h urinary sodium <3 or 3-6 g, respectively. The excretion of 24-h urinary sodium was detected with ion-selective electrode approach. Plasma carnitine and acylcarnitine concentrations were measured with tandem mass spectrometry. Each participant completed the head-up tilt test. The demographics, clinical characteristics, hemodynamic parameters and plasma carnitine and acylcarnitine concentrations were compared between the two groups. A bivariate correlation between plasma acylcarnitine profiles and the excretion of 24-h urinary sodium was conducted with Spearman's correlation coefficients. Of the enrolled VVS patients, 14 patients were assigned to the group of low urinary sodium excretion and the remaining 12 patients were assigned to the group of normal urinary sodium excretion. Symptoms of fatigue were more prevalent in the group of low urinary sodium excretion than in the group of normal urinary sodium excretion ( = 0.009). Aside from fatigue, no other differences in the demographics, clinical characteristics or hemodynamic parameters during the head-up tilt test were found between the two groups ( > 0.05). Concentrations of plasma tiglylcarnitine (C5:1), hydroxyhexadecanoylcarnitine (C16OH), hydroxyoctadecanoylcarnitine (C18OH), and carnitine C22 were significantly higher in the group of low urinary sodium excretion than in the group of normal urinary sodium excretion (all values = 0.048); moreover, they were all negatively correlated with 24-h urinary sodium levels (all -values = 0.016). There were no differences between the two groups in other acylcarnitines or free carnitine. Reduced excretion of 24-h urinary sodium is associated with a disturbed plasma acylcarnitine profile in children with VVS. The findings suggest that restricted sodium intake-induced disturbance of plasma acylcarnitines and related cellular energy metabolism might be involved in the pathogenesis of VVS in children.
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http://dx.doi.org/10.3389/fped.2020.00098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078237PMC
March 2020

Superelastic EGaIn Composite Fibers Sustaining 500% Tensile Strain with Superior Electrical Conductivity for Wearable Electronics.

ACS Appl Mater Interfaces 2020 Feb 24;12(5):6112-6118. Epub 2020 Jan 24.

Department of Biomedical Engineering, School of Medicine , Tsinghua University , Beijing 100084 , China.

Stretchable conductive fibers have gained significant attention in the field of wearable and flexible electronics because of their inherited unique properties. Up to now, there are few reports regarding the highly stretchable fibers with excellent electronic properties. In this work, a highly stretchable fiber with superior electrical conductivity is fabricated, which contains a core fiber, an intermediate modified layer, and an outer eutectic-gallium-indium liquid metal layer. The fiber demonstrates an excellent electrical conductivity of over 10 S cm when stretched up to 500% strain, which is far superior to the existing stretchable conductive fiber. The stretchable conductive fiber shows excellent thermostability with a maximum operating temperature of nearly 250 °C. Such unique fibers can be applied as highly stretchable, deformable conductor to charge a mobile phone, and sensor to monitor human activities. This work offers promising application in the areas of flexible and wearable electronics.
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http://dx.doi.org/10.1021/acsami.9b23083DOI Listing
February 2020

A Highly Stretchable Liquid Metal Polymer as Reversible Transitional Insulator and Conductor.

Adv Mater 2019 Jun 11;31(23):e1901337. Epub 2019 Apr 11.

Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.

Materials with a temperature-controlled reversible electrical transition between insulator and conductor are attracting huge attention due to their promising applications in many fields. However, most of them are intrinsically rigid and require complicated fabrication processes. Here, a highly stretchable (680% strain) liquid metal polymer composite as a reversible transitional insulator and conductor (TIC), which is accompanied with huge resistivity changes (more than 4 × 10 times) reversibly through a tuning temperature in a few seconds is introduced. When frozen, the insulated TIC becomes conductive and recovers after warming. Both the phase change of the liquid metal droplets and the rigidity change of the polymer contribute directly to transition between insulator and conductor. A simplified model is established to predict the expansion and connection of liquid metal droplets. Along with high stretchability, straightforward fabrication methods, rapid triggering time, large switching ratio, good repeatability, the TIC offers tremendous possibilities for numerous applications, like stretchable switches, semiconductors, temperature sensors, and resistive random-access memory. Accordingly, a system that can display numbers and letters via converting alternative TIC temperature to a binary signal on a computer is conceived and demonstrated. The present discovery suggests a general strategy for fabricating and stimulating a stretchable transitional insulator and conductor based on liquid metal and allied polymers.
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http://dx.doi.org/10.1002/adma.201901337DOI Listing
June 2019

Antibodies: The major participants in maternal-fetal interaction.

J Obstet Gynaecol Res 2019 Jan 19;45(1):39-46. Epub 2018 Oct 19.

National Center for Clinical Laboratories, Beijing Hospital, Beijing, China.

The aim of this study is to improve our understanding of the mechanisms involved in maternal-fetal immune tolerance. We searched the related literatures and overviewed the major antibodies associated with pregnancy and described in details their possible roles in mediating maternal-fetal interactions. Antibodies classified into different types based on their functional or structural characteristics were summarized, including immunoglobulin G, blocking antibody, nonprecipitating asymmetric antibody, antiphospholipid antibody, antitrophoblast antibody and antipaternal antibody. The presence and levels of various circulating antibodies in pregnancy may play a crucial role in the occurrence, development and termination of pregnancy.
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http://dx.doi.org/10.1111/jog.13839DOI Listing
January 2019

Sleep Duration Interacts With Lifestyle Risk Factors and Health Status to Alter Risk of All-Cause Mortality: The Rural Chinese Cohort Study.

J Clin Sleep Med 2018 05 15;14(5):857-865. Epub 2018 May 15.

Department of Preventive Medicine, Shenzhen University Health Sciences Center, Shenzhen, Guangdong, People's Republic of China.

Study Objectives: Many studies suggest an association of both short and long sleep duration with all-cause mortality, but the effect of co-occurrence of sleep duration and other lifestyle risk factors or health status remains unclear.

Methods: A total of 17,184 participants aged 18 years or older from rural areas of China were examined at baseline from 2007 to 2008 and followed up from 2013 to 2014. Cox proportional hazard models were used to estimate the hazard ratio (HR) and 95% confidence interval (CI).

Results: During 6-year follow-up, we identified 1,101 deaths. The multivariable-adjusted mortality risk was significantly higher with short-duration sleepers (< 6.5 hours) (HR = 1.37, 95% CI 1.01-1.86) and long-duration sleepers (≥ 9.5 hours) (HR = 1.35, 95% CI 1.05-1.74) versus 6.5-7.5 hours. The multiplicative interaction of long sleep duration with some lifestyle risk factors and health statuses increased the mortality risk in men (low level of physical activity: HR = 1.03, 95% CI 1.02-1.04; hypertension: HR = 1.06, 95% CI 1.04-1.09; type 2 diabetes mellitus [T2DM]: HR = 1.07, 95% CI 1.04-1.11). Similar results were found in women (low level of physical activity: HR = 1.03, 95% CI 1.02-1.05; T2DM: HR = 1.07, 95% CI 1.05-1.10).

Conclusions: Sleep duration could be a predictor of all-cause mortality and its interaction with physical activity, hypertension, and T2DM may increase the risk of mortality.
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http://dx.doi.org/10.5664/jcsm.7124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940438PMC
May 2018

Effect of Oxidation Condition on Growth of N: ZnO Prepared by Oxidizing Sputtering Zn-N Film.

Nanoscale Res Lett 2016 Dec 1;11(1):274. Epub 2016 Jun 1.

Key Laboratory of Electromagnetic Processing of Materials (Ministry of Education), Northeastern University, Shenyang, 110819, China.

Nitrogen-doped zinc oxide (N: ZnO) films have been prepared by oxidizing reactive RF magnetron-sputtering zinc nitride (Zn-N) films. The effect of oxidation temperature and oxidation time on the growth, transmittance, and electrical properties of the film has been explored. The results show that both long oxidation time and high oxidation temperature can obtain the film with a good transmittance (over 80 % for visible and infrared light) and a high carrier concentration. The N: ZnO film exhibits a special growth model with the oxidation time and is first to form a N: ZnO particle on the surface, then to become a N: ZnO layer, and followed by the inside Zn-N segregating to the surface to oxidize N: ZnO. The surface particle oxidized more adequately than the inside. However, the X-ray photoemission spectroscopy results show that the lower N concentration results in the lower N substitution in the O lattice (No). This leads to the formation of n-type N: ZnO and the decrease of carrier concentration. Thus, this method can be used to tune the microstructure, optical transmittance, and electrical properties of the N: ZnO film.
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http://dx.doi.org/10.1186/s11671-016-1485-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889532PMC
December 2016

Abnormal splicing in the N-terminal variable region of cardiac troponin T impairs systolic function of the heart with preserved Frank-Starling compensation.

Physiol Rep 2014 Sep 4;2(9). Epub 2014 Sep 4.

Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan.

Abnormal splice-out of the exon 7-encoded segment in the N-terminal variable region of cardiac troponin T (cTnT-ΔE7) was found in turkeys and, together with the inclusion of embryonic exon (eTnT), in adult dogs with a correlation with dilated cardiomyopathy. Overexpression of these cTnT variants in transgenic mouse hearts significantly decreased cardiac function. To further investigate the functional effect of cTnT-ΔE7 or ΔE7+eTnT in vivo under systemic regulation, echocardiography was carried out in single and double-transgenic mice. No atrial enlargement, ventricular hypertrophy or dilation was detected in the hearts of 2-month-old cTnT-ΔE7 and ΔE7+eTnT mice in comparison to wild-type controls, indicating a compensated state. However, left ventricular fractional shortening and ejection fraction were decreased in ΔE7 and ΔE7+eTnT mice, and the response to isoproterenol was lower in ΔE7+eTnT mice. Left ventricular outflow tract velocity and gradient were decreased in the transgenic mouse hearts, indicating decreased systolic function. Ex vivo working heart function showed that high afterload or low preload resulted in more severe decreases in the systolic function and energetic efficiency of cTnT-ΔE7 and ΔE7+eTnT hearts. On the other hand, increases in preload demonstrated preserved Frank-Starling responses and minimized the loss of cardiac function and efficiency. The data demonstrate that the N-terminal variable region of cardiac TnT regulates systolic function of the heart.
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http://dx.doi.org/10.14814/phy2.12139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270238PMC
September 2014

Simultaneous quantification of three pyranocoumarins of Peucedanum praeruptorum in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study.

J Chromatogr Sci 2015 Apr 11;53(4):511-8. Epub 2014 Jul 11.

Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, PR China

A simple, rapid and robust liquid chromatography-tandem mass spectrometry was established and validated for simultaneous quantifications of three pyranocoumarins (praeruptorin A-C) in rat plasma. Following a single-step liquid-liquid extraction, the analytes were separated on a reversed-phase C18 column with a mobile phase consisting of methanol and 10 mM ammonium acetate solution (70 : 30, v/v) at a constant flow rate of 0.3 mL/min. The linear calibration curves were obtained over the concentration ranges 2.93-1470 ng/mL for praeruptorin A, 1.47-734 ng/mL for praeruptorin B and 2.00-1000 ng/mL for praeruptorin C. The within-batch accuracy was -8.6 to 7.5% for praeruptorin A, -9.5 to 12.0% for praeruptorin B and -10.5 to 12.5% for praeruptorin C, respectively. The between-batch accuracy was -3.5 to 1.4% for praeruptorin A, -8.7 to 3.4% for praeruptorin B and -6.0 to 4.3% for praeruptorin C, respectively. The within-batch and between-batch precisions were ≤13.1 and ≤8.2%, respectively. This method is suitable to simultaneously determine the three pyranocoumarins in plasma and thus to investigate the pharmacokinetics of the pyranocoumarins of Peucedanum praeruptorum in rats.
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http://dx.doi.org/10.1093/chromsci/bmu077DOI Listing
April 2015

Interrogating congenital heart defects with noninvasive fetal echocardiography in a mouse forward genetic screen.

Circ Cardiovasc Imaging 2014 Jan 6;7(1):31-42. Epub 2013 Dec 6.

Department of Developmental Biology, University of Pittsburgh, Pittsburgh, PA.

Background: Congenital heart disease (CHD) has a multifactorial pathogenesis, but a genetic contribution is indicated by heritability studies. To investigate the spectrum of CHD with a genetic pathogenesis, we conducted a forward genetic screen in inbred mice using fetal echocardiography to recover mutants with CHD. Mice are ideally suited for these studies given that they have the same four-chamber cardiac anatomy that is the substrate for CHD.

Methods And Results: Ethylnitrosourea mutagenized mice were ultrasound-interrogated by fetal echocardiography using a clinical ultrasound system, and fetuses suspected to have cardiac abnormalities were further interrogated with an ultrahigh-frequency ultrasound biomicroscopy. Scanning of 46 270 fetuses revealed 1722 with cardiac anomalies, with 27.9% dying prenatally. Most of the structural heart defects can be diagnosed using ultrasound biomicroscopy but not with the clinical ultrasound system. Confirmation with analysis by necropsy and histopathology showed excellent diagnostic capability of ultrasound biomicroscopy for most CHDs. Ventricular septal defect was the most common CHD observed, whereas outflow tract and atrioventricular septal defects were the most prevalent complex CHD. Cardiac/visceral organ situs defects were observed at surprisingly high incidence. The rarest CHD found was hypoplastic left heart syndrome, a phenotype never seen in mice previously.

Conclusions: We developed a high-throughput, 2-tier ultrasound phenotyping strategy for efficient recovery of even rare CHD phenotypes, including the first mouse models of hypoplastic left heart syndrome. Our findings support a genetic pathogenesis for a wide spectrum of CHDs and suggest that the disruption of left-right patterning may play an important role in CHD.
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http://dx.doi.org/10.1161/CIRCIMAGING.113.000451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962690PMC
January 2014

Dose-dependent diastolic dysfunction and early death in a mouse model with cardiac troponin mutations.

J Mol Cell Cardiol 2013 Sep 26;62:227-36. Epub 2013 Jun 26.

Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA.

Our aim was to explore the dose-dependent diastolic dysfunction and the mechanisms of heart failure and early death in transgenic (TG) mice modeling human restrictive cardiomyopathy (RCM). The first RCM mouse model (cTnI(193His) mice) carrying cardiac troponin I (cTnI) R193H mutation (mouse cTnI R193H equals to human cTnI R192H) was generated several years ago in our laboratory. The RCM mice manifested a phenotype similar to that observed in RCM patients carrying the same cTnI mutation, i.e. enlarged atria and restricted ventricles. However, the causes of heart failure and early death observed in RCM mice remain unclear. In this study, we have produced RCM TG mice (cTnI(193His)-L, cTnI(193His)-M and cTnI(193His)-H) that express various levels of mutant cTnI in the heart. Histological examination and echocardiography were performed on these mice to monitor the time course of the disease development and heart failure. Our data demonstrate that cTnI mutation-caused diastolic dysfunction is dose-dependent. The key mechanism is myofibril hypersensitivity to Ca(2+) resulting in an impaired relaxation in the mutant cardiac myocytes. Prolonged relaxation time and delay of Ca(2+) decay observed in the mutant cardiac myocytes are correlated with the level of the mutant protein in the heart. Markedly enlarged atria due to the elevated end-diastolic pressure and myocardial ischemia are observed in the heart of the transgenic mice. In the mice with the highest level of the mutant protein, restricted ventricles and systolic dysfunction occur followed immediately by heart failure and early death. Diastolic dysfunction caused by R193H troponin I mutation is specific, showing a dose-dependent pattern. These mouse models are useful tools for the study of diastolic dysfunction. Impaired diastole can cause myocardial ischemia and fibrosis formation, resulting in the development of systolic dysfunction and heart failure with early death in the RCM mice with a high level of the mutant protein in the heart.
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http://dx.doi.org/10.1016/j.yjmcc.2013.06.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394738PMC
September 2013

Sensitive detection of endonuclease activity and inhibition using gold nanorods.

Biosens Bioelectron 2012 Apr 9;34(1):144-50. Epub 2012 Feb 9.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, China.

It is important to develop reliable and sensitive methods for assay of nuclease activity. With this goal in mind, we report a new strategy for nuclease assay by taking advantage of efficient fluorescence resonance energy transfer (FRET) between gold nanorods (GNRs) and fluorescein-tagged single-stranded DNA (FDNA). Upon mixing with GNRs, the FRET between positively charged GNRs and negatively charged FDNA caused a decrease in fluorescence of FDNA. The formation of FDNA/cDNA duplex further improved the FRET efficiency, leading to a significant decrease in fluorescence intensity. However, fluorescence is restored when FDNA1/cDNA1 hybrid was cleaved into small fragments by EcoRI endonucleases, resulting in a decrease in FRET efficiency because of weakened electrostatic interaction between GNRs and the shortened DNA fragments. Activity of EcoRI endonuclease has been real-time studied by monitoring fluorescence change with the prolonging of interaction time. Under optimized conditions, the cleaved fraction is linear with EcoRI concentration over the range of 1.0×10(-3) to 1.0×10(-1) U μL(-1), with a limit of detection of 6.5×10(-4) U μL(-1) which is much better or at least comparable to previous reports. Site-specific DNA cleavage by EcoRI endonuclease has also been verified by gel electrophoresis, fluorescence anisotropy and TEM analysis, which indicated that this method is a feasible and reasonable approach to study sequence-specific protein-DNA interactions. Assay of BamHI activity demonstrated that it is a more universally applied method for studying the activity of endonuclease. Furthermore, this fluorescence assay has been also used for studying the inhibition of EcoRI endonuclease activity. Importantly, experimental results suggested that endonuclease inhibitors can be screened by monitoring the change of fluorescence change. Therefore, this FRET assay is a simple, sensitive and effective approach to study endonuclease activity and inhibition, and as such, it promises to provide a feasible method to screen nuclease inhibitors.
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http://dx.doi.org/10.1016/j.bios.2012.01.034DOI Listing
April 2012

Label-free fluorescent assay for real-time monitoring site-specific DNA cleavage by EcoRI endonuclease.

Analyst 2012 Apr 22;137(7):1713-7. Epub 2012 Feb 22.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemistry Engineering, Shaanxi Normal University, Xi'an, 710062, China.

DNA cleavage reaction catalyzed by nucleases is essential in many important biological processes and medicinal chemistry. Therefore, it is important to develop reliable and facile methods to assay nuclease activity. With this goal in mind, we report a fluorescent assay for label-free, facile, and real-time monitoring of DNA cleavage by EcoRI endonuclease using SYBR Green I (SGI) as a signal probe. The fluorescence of SGI dramatically increased when the free SGI was mixed with double-stranded DNA (dsDNA) substrate. Upon interacting with EcoRI, which cleaves the dsDNA into small fragments, the weakened interaction between SGI and the shortened DNA fragments caused a decrease in fluorescence of SGI. EcoRI-DNA interaction was real-time studied by monitoring fluorescence change with the prolonging of interaction time. The important kinetic parameters, including Michaelis-Menten constant (K(M)) and maximum initial velocity (V(max)), were accurately calculated, which is consistent with previously reported studies. Site-specific DNA cleavage by EcoRI endonuclease has also been verified by gel electrophoresis analysis, which indicated that this method is a simple and effective approach to assay DNA cleavage reaction. Specificity investigation demonstrated that EcoRI-DNA interactions can be studied with high selectivity. Compared with previously reported methods, this approach is selective, simple, convenient and cost-efficient without any labeling of the probe or of the target.
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http://dx.doi.org/10.1039/c2an16287cDOI Listing
April 2012

Identifying G-quadruplex-binding ligands using DNA-functionalized gold nanoparticles.

Analyst 2012 Apr 14;137(7):1663-8. Epub 2012 Feb 14.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemistry Engineering, Shaanxi Normal University, Xi'an 710062, China.

The G-rich overhang of human telomere tends to form a G-quadruplex structure, and G-quadruplex formation can effectively inhibit telomerase activity in most cancer cells. Therefore, it is important to identify the formation and properties of the G-quadruplex, with the particular aim of selecting G-quadruplex-binding ligands that could potentially lead to the development of anticancer therapeutic agents. With this goal in mind, we report a fluorescence resonance energy transfer (FRET) assay system for the identification of G-quadruplex ligands using DNA-functionalized gold nanoparticles (DNA-GNPs) as the fluorescence quencher and a carboxyfluorescein (FAM)-tagged human telomeric sequence (F-GDNA) as the recognition probe. A thiolated complementary strand of human telomeric DNA (cDNA), which first adheres to the surface of the GNPs and then hybridizes with F-GDNA, results in the fluorescence quenching of F-GDNA by the GNPs. However, fluorescence is restored when single-stranded F-GDNA folds into a G-quadruplex structure upon the binding of quadruplex ligands, leading to the release of F-GDNA from the surface of the GNPs. Combined data from fluorescence measurements and CD spectroscopy indicated that ligands selected by this FRET method could induce GDNA to form a G-quadruplex. Therefore, this FRET G-quadruplex assay is a simple and effective approach to identify quadruplex-binding ligands, and, as such, it promises to provide a solid foundation for the development of novel anticancer therapeutic agents.
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http://dx.doi.org/10.1039/c2an16051jDOI Listing
April 2012

Gold nanorods-based FRET assay for sensitive detection of Pb2+ using 8-17DNAzyme.

Analyst 2011 Dec 26;136(24):5169-74. Epub 2011 Oct 26.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, China.

In this paper, we have reported a sensitive assay for fluorescence "turn-on" detection of Pb(2+) in aqueous solutions based on FRET between gold nanorods (GNRs) and the FAM-labeled substrate strand of 8-17DNAzyme. The fluorescence of the FAM-labeled substrate strand is quenched when 8-17DNAzyme is adsorbed on GNRs surface through electrostatic interaction. In the presence of lead ions, the fluorescence is restored due to the decrease of FRET efficiency caused by the specific cleavage of the FAM-labeled substrate strand by the enzyme, which weakens the electrostatic interaction between the GNRs and short FAM-labeled DNA fragment. The interference of eleven common metal ions has been tested, indicating that Pb(2+) can be selectively detected. This method exhibits a high sensitivity for Pb(2+) with a detection limit of 61.8 pM and a linear range from 0.1 nM to 100 nM. It is a simple, sensitive, and selective method for Pb(2+) detection. Moreover, this sensing system obtained satisfying results for Pb(2+) detection in tap water samples.
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http://dx.doi.org/10.1039/c1an15783cDOI Listing
December 2011

Gold nanorods-based FRET assay for ultrasensitive detection of Hg2+.

Chem Commun (Camb) 2011 Dec 25;47(46):12500-2. Epub 2011 Oct 25.

Key Laboratory of Applied Surface and Colloid Chemistry, Shaanxi Normal University, Ministry of Education, Xi'an, 710062, PR China.

A fluorescence method for detecting mercury ion in a homogeneous medium is proposed with gold nanorods (GNRs) as a fluorescence quencher on the basis of the fluorescence resonance energy transfer (FRET). Under the optimum conditions, the method exhibits a dynamic response range from 10 pM to 5 nM with a detection limit of 2.4 pM.
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http://dx.doi.org/10.1039/c1cc15084gDOI Listing
December 2011

Inhibition of p300-HAT results in a reduced histone acetylation and down-regulation of gene expression in cardiac myocytes.

Life Sci 2010 Dec 26;87(23-26):707-14. Epub 2010 Oct 26.

Heart Centre, Children's Hospital of Chongqing Medical University, Chongqing, PR China.

Aims: Histone acetylation plays an important role in cardiogenesis, but the underlying mechanism is unclear. In this study, we investigated the relationship between histone hypo-acetylation and the expression of cardiac-specific genes to explore the underlying mechanisms.

Main Methods: Cardiac-specific genes that physically interacted with p300 protein in mouse hearts were analyzed using chromatin immunoprecipitation (ChIP) assays. The cultured mouse neonatal cardiac myocytes were treated with curcumin with different concentrations and durations. The changes of histone acetyltransferase (HAT) activities, histone acetylation, cardiac-specific genes expression, and structure of chromatin were assessed by ELISA, Western blotting, quantitative RT-PCR, and ChIP assays, respectively.

Key Findings: Results from the ChIP assay showed that GATA4, Nkx2.5, and Mef2c physically interacted with p300 protein. After treatment with 30 μM curcumin for 24h, the HAT activities of cardiac myocytes were inhibited significantly. And the acetylation of whole histone H3 was reduced by 0.3983-fold compared to control groups (P<0.05). Accordingly, the expression of cardiac-specific genes, GATA4, Nkx2.5, and Mef2c, were significantly down-regulated. Acetylation of histone H3 bound with promoter regions of these genes was significantly reduced.

Significance: p300 interacts with cardiac-specific genes, GATA4, Nkx2.5 and Mef2c, and inhibition of p300-HAT by curcumin down-regulates their expression through the inhibition of histone H3 acetylation in the promoter regions. This finding indicates that p300-HAT mediated histone H3 acetylation plays an important role in the regulation of cardiac gene expression, which is a novel mechanism of epigenetic regulation in the heart during the development and in case of some congenital heart diseases.
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http://dx.doi.org/10.1016/j.lfs.2010.10.009DOI Listing
December 2010

Deficiency of methionine sulfoxide reductase A causes cellular dysfunction and mitochondrial damage in cardiac myocytes under physical and oxidative stresses.

Biochem Biophys Res Commun 2010 Nov 28;402(4):608-13. Epub 2010 Oct 28.

College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA.

Methionine sulfoxide reductase A (MsrA) is an enzyme that reverses oxidation of methionine in proteins. Using a MsrA gene knockout (MsrA(-/-)) mouse model, we have investigated the role of MsrA in the heart. Our data indicate that cellular contractility and cardiac function are not significantly changed in MsrA(-/-) mice if the hearts are not stressed. However, the cellular contractility, when stressed using a higher stimulation frequency (2Hz), is significantly reduced in MsrA(-/-) cardiac myocytes. MsrA(-/-) cardiac myocytes also show a significant decrease in contractility after oxidative stress using H(2)O(2). Corresponding changes in Ca(2+) transients are observed in MsrA(-/-) cardiomyocytes treated with 2Hz stimulation or with H(2)O(2). Electron microscope analyses reveal a dramatic morphological change of mitochondria in MsrA(-/-) mouse hearts. Further biochemical measurements indicate that protein oxidation levels in MsrA(-/-) mouse hearts are significantly higher than those in wild type controls. Our study demonstrates that the lack of MsrA in cardiac myocytes reduces myocardial cell's capability against stress stimulations resulting in a cellular dysfunction in the heart.
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http://dx.doi.org/10.1016/j.bbrc.2010.10.064DOI Listing
November 2010

Ethanol and its metabolites induce histone lysine 9 acetylation and an alteration of the expression of heart development-related genes in cardiac progenitor cells.

Cardiovasc Toxicol 2010 Dec;10(4):268-74

Department of Cardiology, The Children's Hospital of Chongqing Medical University, 136 Zhong Shan Er Road, Yu Zhong District, 400014 Chongqing, People's Republic of China.

Alcohol exposure during pregnancy may cause congenital heart disease (CHD), but the underlying mechanisms are not clear. Recent evidence suggests that ethanol and its metabolites can selectively increase histone H3 acetylation at lysine 9 (H3AcK9) residue in rat hepatocytes. This may be a mechanism by which ethanol alters gene expression. The goal of current study is to investigate the effect of ethanol and its metabolites on H3AcK9 acetylation and the mRNA expression of heart development-related genes (GATA4, Mef2c, and Tbx5) in cardiac progenitor cells. We used mitochondrial activity (MTT) assay to assess the viability of cardiac progenitor cells. Western blotting and real-time PCR were employed to determine H3AcK9 acetylation and gene expression. Low levels of ethanol (50 mM), acetaldehyde (4 mM), and acetate (4 mM) had no effect on cell proliferation. However, high concentrations of ethanol (200 mM), acetaldehyde (12 mM), and acetate (16 mM) reduced cell viability by 30%, respectively (P < 0.05). Low levels of ethanol and acetate increased the acetylation of H3 lysine 9 by 2.4- and 2.2-fold, respectively (P < 0.05), but did not significantly change the expression of the heart development-related genes. High concentrations of ethanol and acetate increased H3 lysine 9 acetylation by 5.3- and 5.6-fold, respectively (P < 0.05). Moreover, high levels of ethanol and acetate significantly augmented the expression of GATA4 and Mef2c. Conversely, acetaldehyde (4 or 12 mM) had little effect on H3 lysine 9 acetylation or the expression of the heart development-related genes. Our studies demonstrate that high levels of ethanol or its metabolites induce H3AcK9 acetylation and impair cardiac progenitor cells. The altered histone H3 acetylation at lysine 9 has an important impact on the expression of the heart development-related genes, which may be one of the mechanisms underlying the alcohol-induced CHD.
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http://dx.doi.org/10.1007/s12012-010-9081-zDOI Listing
December 2010

Three-dimensional echocardiographic virtual endoscopy for the diagnosis of congenital heart disease in children.

Int J Cardiovasc Imaging 2010 Dec 10;26(8):851-9. Epub 2010 Jun 10.

Department of Pediatric Cardiology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

Virtual endoscopy (VE) is a new post-processing method that uses volumetric data sets to simulate the tracks of a "conventional" flexible endoscope. However, almost all studies of this method have involved virtual visualizations of the cardiovascular structures applied to computed tomography (CT) and magnetic resonance (MR) datasets. This paper introduces a novel visualization method called the "three-dimensional echocardiographic intracardiac endoscopic simulation system (3DE IESS)", which uses 3D echocardiographic images in a virtual reality (VR) environment to diagnose congenital heart disease. The aim of this study was to analyze the feasibility of VE in the evaluation of congenital heart disease in children and its accuracy compared with 2DE. Three experienced pediatric cardiologists blinded to the patients' diagnoses separately reviewed 40 two-dimensional echocardiographic (2DE) datasets and 40 corresponding VE datasets and judged whether abnormal intracardiac anatomy was present in terms of a five-point scale (1 = definitely absent; 2 = probably absent; 3 = cannot be determined; 4 = probably present; and 5 = definitely present). Compared with clinical diagnosis, the diagnostic accuracy of VE was 98.7% for ASD, 92.4% for VSD, 92.6% for TOF, and 94% for DORV, respectively. Diagnostic accuracy of VE was significantly higher than that of 2DE for TOF and DORV except for ASD and VSD. The receiver operating characteristic (ROC) curve for VE was closer to the optimal performance point than was the ROC curve for 2DE. The area under the ROC curve was 0.96 for VE and 0.93 for 2DE. Kappa values (range, 0.73-0.79) for VE and 2DE indicated substantial agreement. 3D echocardiographic VE can enhance our understanding of intracardiac structures and facilitate the evaluation of congenital heart disease.
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http://dx.doi.org/10.1007/s10554-010-9649-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2991166PMC
December 2010

Spatiotemporal expression of histone acetyltransferases, p300 and CBP, in developing embryonic hearts.

J Biomed Sci 2009 Feb 23;16:24. Epub 2009 Feb 23.

Department of Cardiology, The Children's Hospital of Chongqing Medical University, Chongqing, PR China.

Histone acetyltransferases (HATs), p300 and cAMP response element binding protein (CREB)-binding protein (CBP) are two structurally related transcriptional co-activators that activate expression of many eukaryotic genes involved in cellular growth and signaling, muscle differentiation and embryogenesis. However, whether these proteins play important and different roles in mouse cardiogenesis is not clear. Here, we investigate the protein distributions and mRNA expression of the two HATs in embryonic and adult mouse heart during normal heart development by using immunohistochemical and RT-PCR techniques. The data from immunohistochemical experiments revealed that p300 was extensively present in nearly every region of the hearts from embryonic stages to the adulthood. However, no CBP expression was detected in embryonic hearts at day E7.5. CBP expression appeared at the later stages, and the distribution of CBP was less than that of p300. In the developmental hearts after E10.5, both for p300 and CBP, the mRNA expression levels reached a peak on day E10.5, and then were gradually decreased afterwards. These results reveal that both p300 and CBP are related to embryonic heart development. The dynamic expression patterns of these two enzymes during mouse heart development indicate that they may play an important role on heart development. However, there is a difference in spatiotemporal expression patterns between these two enzymes during heart development. The expression of p300 is earlier and more predominate, suggesting that p300 may play a more important role in embryonic heart development especially during cardiac precursor cell induction and interventricular septum formation.
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http://dx.doi.org/10.1186/1423-0127-16-24DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2653528PMC
February 2009

In vitro validation of right ventricular volume and mass measurement by real-time three-dimensional echocardiography.

Echocardiography 2006 May;23(5):395-9

Cardiovascular Center, Children's Hospital of Fudan University, Shanghai, China.

Objective: To evaluate initially the feasibility and accuracy of real-time three-dimensional echocardiography (RT-3DE) for quantifying right ventricular (RV) volume and wall mass in an in vitro experimental study.

Methods: In ten excised porcine hearts, measurements of RV volume and free wall mass with RT-3DE were outlined and calculated by 2-, 4-, 8- and 16-plane methods with Tom Tec 4D Cardio-View RT 1.0. The results were compared with those of 2D length method and 2D biplane Simpson method. The values of RV silicone latex cast and free wall mass measured by water displacement were served as reference values.

Results: RV shapes of excised porcine hearts with RT-3DE were similar to those of the actual anatomic RVs and RV silicone latex casts. From the findings of analysis of variance and Student-Newman-Keuls test, there was no significant difference between measurements of RV volume with RT-3DE 16-plane (mean 64.05 ml), 8-plane (61.83 ml) and the reference values of RV silicone latex casts (62.94 ml). No significant difference was found between measurements of RV free wall mass with 16-plane (72.81 g), 8-plane (71.05 g) and the reference values of RV free wall masses (76.21 g). However, there was significant difference between measurements of RV volume and free wall mass with 2-plane, 2D biplane Simpson method and the reference values. Furthermore, the measurements of RV volume and free wall mass with 16-plane and 8-plane were better correlated with the reference values than those with 4-plane and 2D length method.

Conclusions: RT-3DE will be a valuable technique for quantifying irregular crescentic RV volume and wall mass.
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http://dx.doi.org/10.1111/j.1540-8175.2006.00221.xDOI Listing
May 2006
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