Publications by authors named "Guoyao Wang"

6 Publications

  • Page 1 of 1

Use of the O-Z Flap to Repair Scalp Defects After Cancer Tumor Resection.

J Craniofac Surg 2022 May 26;33(3):892-894. Epub 2021 Oct 26.

Ningbo City First Hospital, Ningbo, China.

Background: Large benign and malignant tumors in the scalp cannot be sutured directly after resection. Instead, skin grafting or skin flap repair is the most commonly used techniques. Local tissue depression and lack of hair growth are some of the drawbacks associated with these techniques. The use of a modified local flap (the O-Z flap) may effectively overcome these issues.

Objective: To explore the application of O-Z flap in wound repair after excision of benign and malignant tumors of the scalp.

Methods: Between April 2016 and November 2017, the authors treated 6 patients with scalp tumors. They underwent round or oval radical tumor resection with negative margins. Tumor specimens were diagnosed by cryosection during operation. According to the wound defect size and location, surrounding scalp looseness, and hair distribution, 2 rotating flaps in opposite directions were formed on the left and right sides or front and back of the wound. Subsequently, the skin flaps were rotated in opposite directions to repair the wound.

Results: The scalp tumors comprised 2 cases of basal cell carcinoma, 2 cases of squamous cell carcinoma, 1 case of hair sheath carcinoma, and 1 case of epidermoid cyst. After complete tumor resection, the wound defect area was between 3.0 cm × 3.5 cm and 5.0 cm × 6.0 cm. After operation, approximately 6% of the tip of the skin flap was necrotized. The wounds healed after 4 weeks of dressing treatment. All skin flaps survived in stage I and no complications occurred. All patients were followed up for 3 to 12 months; the scalps were in good condition and no tumor recurrence was found.

Conclusions: The use of the O-Z flap to repair scalp wounds offers flexible design, good blood circulation, uniform tension, and good hair growth after operation; thus, this technique is suitable for wound repair following scalp tumor resection.
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http://dx.doi.org/10.1097/SCS.0000000000008300DOI Listing
May 2022

MicroRNA‑106b functions as an oncogene and regulates tumor viability and metastasis by targeting LARP4B in prostate cancer.

Mol Med Rep 2019 Aug 5;20(2):951-958. Epub 2019 Jun 5.

Department of Urology, Ningbo First Hospital, Ningbo, Zhejiang 315010, P.R. China.

Prostate cancer (PCa) is the most common malignancy among males worldwide, and is one of the leading causes of cancer‑related mortality. MicroRNAs (miRs) are a type of endogenous, noncoding RNA that serve a key role in pathological processes, and have been demonstrated to be involved in the formation and progression of PCa. Previous studies have reported that miR‑106b acts as an oncogene; however, the specific effects of miR‑106b on PCa have not been fully elucidated. The present study aimed to investigate the role and underlying molecular mechanisms of miR‑106b in the initiation and progression of PCa. In this study, miR‑106b was reported to be overexpressed and la‑related protein 4B (LARP4B) was downregulated in PCa tissues compared with paracancerous tissues. In addition, LARP4B was identified as a target gene of miR‑106b by bioinformatics prediction analysis and a dual luciferase reporter gene assay. Furthermore, MTT, wound healing and Transwell assays were performed to evaluate PCa cell viability, and migration and invasive abilities. The data revealed that inhibition of miR‑106b significantly suppressed the viability, migration and invasion of PCa cells. In addition, inhibition of miR‑106b significantly suppressed the mRNA and protein expression of cancer‑related genes, including matrix metalloproteinase‑2, cluster of differentiation 44 and Ki‑67, and increased that of the tumor suppressor, mothers against decapentaplegic homolog 2. Collectively, the findings of the present study indicated that miR‑106b may target LAR4B to inhibit cancer cell viability, migration and invasion, and may be considered as a novel therapeutic target in PCa.
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http://dx.doi.org/10.3892/mmr.2019.10343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6625195PMC
August 2019

Choosing the appropriate ShangRing size for paediatric circumcision using the no-flip technique.

J Paediatr Child Health 2018 01 11;54(1):42-48. Epub 2017 Aug 11.

Department of Urology, Ningbo First Hospital, Ningbo Hospital of Zhejiang University, Ningbo, Zhejiang, China.

Aim: To determine the correct ShangRing size for paediatric circumcision using the no-flip technique.

Methods: A cohort of 104 boys (from 6 to 14 years) underwent ShangRing circumcision at Ningbo First Hospital, China. The patients were randomly divided into three groups according to the different methods used for choosing the ring size. For Group A, the ring size was chosen using the ShangRing measuring tape; for Group B, ring size was chosen based on the diameter of penis measured using a self-designed scale plate; and for Group C, ring size was chosen based on the diameter of the glans measured using the self-designed scale plate. The surgery duration, foreskin dorsal slit rate, intra-operative and post-surgery pain score, pain duration, post-surgery ring detachment duration, pain score for ring detachment, complication rate and satisfaction rate were compared.

Results: Group A had the highest dorsal slit rate and the longest surgical period. There were no significant differences in intra-/post-surgery pain or ring detachment duration among the three groups. The post-surgery oedema rate and foreskin asymmetry occurrence rate was the highest in Group A at 40.62 and 21.88%, respectively. Group C had the highest satisfaction rate (97.14%), with a significant difference from Group A (75.00%). Oedema and asymmetry of the foreskin increased, while the satisfaction rate simultaneously decreased when a larger ShangRing was chosen.

Conclusions: The size of the ShangRing should be chosen in accordance with the diameter of the glans in paediatric ShangRing circumcision using the no-flip technique.
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http://dx.doi.org/10.1111/jpc.13660DOI Listing
January 2018

Application of sequential factorial design and orthogonal array composite design (OACD) to study combination of 5 prostate cancer drugs.

Comput Biol Chem 2017 Apr 4;67:234-243. Epub 2017 Feb 4.

School of Biomedical Engineering, Institute for Personalized Medicine, Shanghai Jiao Tong University, Shanghai 200030, China. Electronic address:

Prostate cancer is one of the most common cancers among men in the United States. It is also a major leading cause of cancer death among men of all races. In order to treat prostate cancer, drug combinations are often applied. Drug combinations target at different pathways of cells can potentially lead to higher efficacy and lower toxicity due to drug synergy. In this paper, we sequentially applied a two-level design and a follow-up orthogonal array composite design (OACD) to investigate combinations of five anti-cancer drugs, namely, doxorubicin, docetaxel, paclitaxel, cis-dichlorodiamine platinum and dihydroartemisinin. Our initial screening using a two-level full factorial design identified doxorubicin and docetaxel as the most significant drugs. A follow-up experiment with an OACD revealed more complicated drug interactions among these 5 anti-cancer drugs. Quadratic effects of doxorubicin and paclitaxel appeared to be significant. A further investigation on contour plots of all the two-drug pairs indicated that combination of doxorubicin and docetaxel are the most effective companion, while the combination of cis-dichlorodiamine platinum and dihydroartemisinin showed unknown antagonistic effects which diminished the individual drug anti-cancer efficacy. These observations have significant practical implications in the understanding of anti-cancer drug mechanism that can facilitate clinical practice of better drug combinations.
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http://dx.doi.org/10.1016/j.compbiolchem.2017.01.010DOI Listing
April 2017

A Novel Approach of Microscopic Subinguinal Varicocelectomy With a "Pulling" Strategy.

Urology 2017 06 29;104:97-101. Epub 2016 Dec 29.

Department of Urology, Zhejiang University Ningbo Hospital, Ningbo First Hospital, Ningbo, Zhejiang, China. Electronic address:

Objective: To investigate the safety and efficacy of a novel approach of microscopic subinguinal varicocelectomy with a "pulling" strategy.

Materials And Methods: Fifty-two male patients with varicocele were enrolled in this study. Twenty-six patients underwent conventional microscopic subinguinal varicocelectomy, whereas the remaining 26 patients accepted our novel approach of treatment. In this method, with a subinguinal incision, we dissociated the spermatic cord from the external ring. And thus we pulled the spermatic cord down and away from the external ring and exposed the spermatic cord that initially was proximal to the external ring in the surgical field. The remainder of the procedure was similar to that of the conventional method. Follow-up was made at 1 and 3 months. Postoperative complications were recorded and sperm parameters were evaluated by semen analysis.

Results: Fewer vein branches were ligated in the novel method group compared with the conventional method group (6.9 ± 2.1 vs 9.8 ± 2.2, P < .001; t = -5.316). There was no significant difference in the number of testicular arteries or lymphatic ducts between the 2 groups. The mean microsurgical operative time in the novel method group was significantly shorter than that in the conventional method group (34.7 ± 6.5 minutes vs 45.7 ± 8.8 minutes, P < .001; t = -5.621). The incidence of postoperative complications was similar for the 2 groups. There was no significant difference regarding semen quality improvement between the 2 groups.

Conclusion: This study demonstrates that our novel approach of microscopic subinguinal varicocelectomy with a "pulling" strategy significantly reduces the number of internal veins to ligate and shortens operating time, with no increase in complications. It is a safe and effective method of varicocele treatment.
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http://dx.doi.org/10.1016/j.urology.2016.12.037DOI Listing
June 2017

Methods for Broadband Spectral Analysis: Intrinsic Fluorescence Temperature Sensing as an Example.

Appl Spectrosc 2017 Jun 24;71(6):1256-1262. Epub 2016 Nov 24.

2 Optical Technology Research Laboratory, Victoria University, Melbourne City, Victoria, Australia.

A systematic study was performed on the temperature-dependent fluorescence of (Ba,Sr)SiO:Eu. The barycenter and extended intensity ratio techniques were proposed to characterize the broadband fluorescence spectra. These techniques and other known methods (listed below) were employed and compared in the fluorescent temperature sensing experiment. Multiple sensing functions were obtained using the behaviors of: (1) the barycenter location of the emission band; (2) the emission bandwidth; and (3) the ratio of intensities at different wavelengths in the emission band, respectively. The barycenter technique was not limited by the spectrometer resolution and worked well while the peak location method failed. All the sensing functions were based on the intrinsic characteristics of the fluorescence of the phosphor and demonstrated nearly linear relationships with temperature in the measuring range. The multifunctional temperature-sensing abilities of the phosphor can be applied in a point thermometer or thermal mapping. The new techniques were validated successfully for characterizing various spectra.
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http://dx.doi.org/10.1177/0003702816678868DOI Listing
June 2017
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