Publications by authors named "Guotian Pei"

6 Publications

  • Page 1 of 1

Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3.

Cancer Manag Res 2021 19;13:1767-1776. Epub 2021 Feb 19.

Department of Thoracic Surgery, Peking University People's Hospital, Beijing, 100044, People's Republic of China.

Objective: This study set out to investigate the effect of on lung cancer progression through targeted regulation of .

Methods: RT-PCR was employed to detect the expression levels in lung cancer cells and its targeted gene mRNA determined by biological information prediction. MTT, invasion and apoptosis rate tests were employed to detect the proliferation, invasion and apoptosis rate of lung cancer cells over-expressing or those with low expression of and the expression of related proteins.

Results: RT-qPCR results manifested that the level was down-regulated in lung cancer tissues and cells, and the expression increased. The and expression levels were negatively correlated. was correlated with tumor differentiation degree, TNM stage and lymph node metastasis of lung cancer patients. Cell tests confirmed that played a tumor-inhibiting function, including inhibiting proliferation and invasion of lung cancer cells and promoting apoptosis. Bioinformatics prediction and subsequent experiments proved that was the direct target of . Moreover, after the expression in lung cancer cells was knocked down, proliferation and invasion of those cells were inhibited dramatically, the apoptosis rate increased markedly, and the expression levels of pro-apoptosis-related proteins and were up-regulated, and the anti-apoptosis-related protein expression was down-regulated.

Conclusion: can inhibit the proliferation and invasion of lung cancer cells and promote their apoptosis by targeting . It can be used as a new potential target for lung cancer treatment.
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http://dx.doi.org/10.2147/CMAR.S251679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903947PMC
February 2021

Unusual metachronous lung adenocarcinomas harboring EGFR L858R/T790M mutations: A case report.

Thorac Cancer 2020 10 12;11(10):3020-3023. Epub 2020 Aug 12.

Department of Thoracic Surgery, Beijing Haidian Hospital, Haidian Section of Peking University Third Hospital, Beijing, China.

Multiple primary lung cancer (MPLC) is defined as two or more primary lung cancers occurring in the same patient and can be classified as synchronous multiple primary lung cancer (sMPLC) and metachronous multiple primary lung cancer (mMPLC). Due to various clinicopathological characteristics and genetic features, MPLC is increasingly encountered in clinical practice. The distinction between MPLC and intrapulmonary metastasis (IM) is of great importance to clinical treatment and prognosis. However, there are currently no golden diagnostic criteria for MPLC due to tumor heterogeneity. Here, we report the case of a patient with four lung cancers (tumor 1, named T1, in the right middle lobe seven years earlier; tumor 2, named T2, in the left lower lobe; tumor 3 and tumor 4, named T3 and T4, in the left upper lobe) and two tumors (T1 and T2) which shared the mutation in epidermal growth factor receptor (EGFR) L858R/T790M based on targeted multigene sequencing, which indicate that these two tumors might have originated from a common ancestor. However, based on previously published guidelines, these three tumors (T2T4) were diagnosed as mMPLC.
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http://dx.doi.org/10.1111/1759-7714.13618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7529550PMC
October 2020

The safety and feasibility of intraoperative near-infrared fluorescence imaging with indocyanine green in thoracoscopic sympathectomy for primary palmar hyperhidrosis.

Thorac Cancer 2020 04 15;11(4):943-949. Epub 2020 Feb 15.

Department of Thoracic Surgery, Beijing Haidian Hospital (Haidian Section of Peking University Third Hospital), Beijing, China.

Background: We investigated the safety and feasibility of intraoperative near-infrared (NIR) imaging using indocyanine green (ICG) during sympathectomy in the management of primary palmar hyperhidrosis (PPH).

Methods: We performed a retrospective review of 142 patients (ICG group) who underwent endoscopic thoracic sympathectomy (ETS) between February 2018 and April 2019. All patients received a 5 mg/kg infusion of ICG 24 hours preoperatively. The vital signs before and after ICG injection and adverse reactions were recorded. Meanwhile, 498 patients (Non-ICG group) who underwent ETS by normal thoracoscopy during August 2017 to April 2019 were also reviewed to compare the abnormal white blood cell (WBC) counts, alanine transaminase (ALT), aspartate transaminase (AST), blood urea nitrogen (BUN), and creatinine (Cr) levels before and after operation between two groups.

Results: For ICG group, the vital signs including body temperature, heart rate and blood pressure before and after ICG injection were stable. There was no significant difference in the abnormal WBC counts, ALT, AST, BUN, and Cr levels before and after operation between two groups. Only one patient had mild adverse reaction (0.7%) after ICG injection. The visibility rate of all sympathetic ganglions was 96.7% (1369/1415). The visibility rate from T1 to T5 was 98.23% (278/283), 98.23% (278/283), 97.17% (275/283), 95.76% (271/283), and 94.35% (267/283), respectively. There was no significant difference in the visibility rate with regard to age, gender, height, weight, body mass index, and PPH grade.

Conclusions: NIR fluorescence imaging with ICG for identifying sympathetic ganglions is relatively safe and feasible.

Key Points: • Significant findings of the study. NIR fluorescence imaging with ICG for identifying sympathetic ganglions is relatively safe and feasible. • What this study adds. This technology may take the place of the rib-oriented method as standard practice for the precise localization of sympathetic ganglions, and may improve the effect of sympathectomies.
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http://dx.doi.org/10.1111/1759-7714.13345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113049PMC
April 2020

Giant mediastinal thymolipoma in a patient with Gardner's syndrome.

Thorac Cancer 2015 Nov 26;6(6):808-11. Epub 2015 Jan 26.

Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University Beijing, China.

Gardner's syndrome is a hereditary disorder inherited as an autosomal dominant with high penetrance and variable expression that is caused by a mutation of the adenomatous polyposis coli gene. It is characterized by gastrointestinal polyps associated with multiple osteomas, dental anomalies, and skin and soft tissue tumors. We present a case of 30-year-old female patient with Gardner's syndrome who presented with a giant mediastinal thymolipoma. The tumor was completely excised through a bilateral posterolateral thoracotomy. There was no recurrence after 20 months of follow-up. We therefore suggest that physicians who regularly treat patients with Gardner's syndrome carefully examine for thoracic manifestations.
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http://dx.doi.org/10.1111/1759-7714.12231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632938PMC
November 2015

Sleeve lobectomy by video-assisted thoracic surgery versus thoracotomy for non-small cell lung cancer.

J Cardiothorac Surg 2015 Sep 10;10:116. Epub 2015 Sep 10.

Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Machang 97, Tongzhou District, Beijing, 101149, China.

Background: Both video-assisted thoracic surgery (VATS) and thoracotomy are used for sleeve lobectomy for patients with non-small cell lung cancer (NSCLC). This retrospective study aimed to assess the safety and efficacy of VATS sleeve lobectomy for NSCLC patients.

Methods: Between May 2009 and May 2013, 51 sleeve lobectomies (10 by VATS and 41 by thoracotomy) were performed for patients with NSCLC. Operative characteristics and postoperative course were compared between two groups.

Results: Patient demographics were similar between the two groups. Thoracotomy patients had larger tumors compared with VATS patients (p = 0.02). VATS patients had a longer operating time (p < 0.001) but a shorter length of postoperative hospital stay (p = 0.009). The two groups did not differ in pathologic stage, histologic results, blood loss, ICU stay, amount of chest drainage, duration of chest drainage, numbers and distributions of dissected lymph nodes and the occurrence of complications. There were no perioperative deaths in the VATS group, whereas there was one death (2.4 %) in the thoracotomy group. There were no conversions to thoracotomy in the VATS group. The overall median survival between the two groups was similar (3.2 years VATS versus 3.2 years thoracotomy, log-rank p = 0.58).

Conclusions: VATS sleeve lobectomy for the treatment of NSCLC is technically feasible and safe and is associated with comparable complication rates and survival compared with thoracotomy approach, but it deserves further investigation in large series.
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http://dx.doi.org/10.1186/s13019-015-0318-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564953PMC
September 2015

Risk factors for postoperative complications after lung resection for non-small cell lung cancer in elderly patients at a single institution in China.

J Thorac Dis 2014 Sep;6(9):1230-8

Department of Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.

Objective: The purpose of this study was to assess the postoperative complications after lung resection for non-small cell lung cancer (NSCLC) in elderly patients and to identify possible associated risk factors.

Methods: All patients aged 70 years or older who underwent pulmonary resection for NSCLC by either an open approach or by a thoracoscopic approach between January 2003 and December 2013 at our institution were reviewed. Postoperative events were divided into minor and major complications. Risk factors for complications were assessed by univariate and multivariate logistic regression analysis. A matched case-control study was performed to determine if the utilization of video-assisted thoracic surgery (VATS) for lung resection for NSCLC in elderly patients' results in decreased complications compared with thoracotomy.

Results: During the study period, 476 consecutive patients (410 thoracotomy, 66 thoracoscopy) older than 70 years underwent resection for NSCLC. Postoperative complications occurred in 169 patients (35.5%) and the overall operative mortality was 2.3% (11 patients). Univariate predictors of complications included history of smoking (P=0.032), CCI scores ≥3 (P<0.001), pneumonectomy (P=0.016), as well as the duration of surgery (P=0.003). After multiple logistic regression analysis, CCI scores ≥3 [odds ratio (OR) =29.95, P<0.001], pneumonectomy (OR =2.26, P=0.029) and prolonged surgery (≥180 min) (OR =1.93, P=0.003) remained the only independent risk factors. After matching based on age, gender, the Charlson Comorbidity Index (CCI), pathologic stage, and the type of resection, there were 60 patients in each group. Patients had similar preoperative characteristics. A VATS approach resulted in a significantly lower rate of complications (25.0% vs. 43.3%, P=0.034) and a shorter median length of stay (19 days, range, 12 to 35 vs. 21 days, range, 13 to 38, P=0.013) compared with thoracotomy.

Conclusions: Pulmonary resection for NSCLC in patients older than 70 years shows acceptable morbidity and mortality. Postoperative complications are more likely to develop in patients with CCI scores ≥3, those who undergo pneumonectomy, and those with a prolonged surgery. Thoracoscopic minimally invasive surgery for NSCLC in elderly patients is associated with fewer complications as well as a shorter hospital stay compared with thoracotomy.
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http://dx.doi.org/10.3978/j.issn.2072-1439.2014.07.23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178082PMC
September 2014