Publications by authors named "Guoli Yang"

57 Publications

Diagnostic potential of trace metals concentration in expressed prostatic secretion and serum of patients with category IV prostatitis.

J Trace Elem Med Biol 2021 Jul 8;68:126819. Epub 2021 Jul 8.

Scientific Research Center, Guilin Medical University, Guilin, Guangxi, China; Guangxi Health Commission Key Laboratory of Disease Proteomics Research, China. Electronic address:

Background: The National Institutes of Health (NIH) category IV prostatitis is a painless prostate gland inflammation, just as its name implies, this type of prostatitis is related with inflammation of the prostate, but most men are not conscious of it. However, category IV prostatitis is fairly common in general populations and reported having indirect relationships with prostate cancer.

Method: We analyzed the concentration of zinc (Zn), copper (Cu), calcium (Ca) and magnesium (Mg) in expressed prostatic secretion (EPS) and serum of patients with category IV prostatitis and healthy controls, investigating the diagnostic potential of different metals in category IV prostatitis using a flame atomic absorption spectrometer (FAAS).

Results: Metal concentration combined clinical characteristics analysis suggested that average level of Zn, Ca, Mg were significantly lower in the EPS of patients with category IV prostatitis (P-value< 0.000), while Cu level raised obviously (P-value< 0.000). And in the serum, mean concentrations of Ca was also found to increase significantly in the patients with category IV prostatitis compared to healthy controls. Moreover, the correlation analysis indicated that age showed a positive correlation with EPS Zn, Ca, Mg concentration (P-value< 0.05), while albumin correlates with EPS Zn, Ca, Mg concentration reversely (P-value< 0.05) in patients with category IV prostatitis.

Conclusion: Our report revealed that determination of the metal elements zinc, copper, calcium and magnesium in the serum and EPS could be a new and promising strategy for the rapid diagnosis of category IV prostatitis.
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http://dx.doi.org/10.1016/j.jtemb.2021.126819DOI Listing
July 2021

Caspase-3 and gasdermin E detection in peri-implantitis.

Biochim Biophys Acta Mol Basis Dis 2021 Nov 14;1867(11):166217. Epub 2021 Jul 14.

Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310006, China. Electronic address:

Peri-implantitis could lead to progressive bone loss and implant failure; however, the mechanism of peri-implantitis remains unclear. Based on emerging evidence, pyroptosis, a novel proinflammatory programmed death, contributes to different oral infectious diseases. In the present study, we investigated the involvement of cleaved caspase-3 and gasdermin E (GSDME) in peri-implantitis and established a pyroptosis model in vitro. By collecting and examining the inflamed biopsies around peri-implantitis, we found that the pyroptosis-related markers (caspase-3, GSDME, and IL-1β) were enhanced relative to levels in control individuals. Furthermore, human gingival epithelium cells (HGECs) induced by tumor necrosis factor-α (TNF-α) exhibited pyroptosis morphological changes (cell swelling and balloon-shaped bubbles) and upregulated expression of pyroptosis-related markers. Pretreated with Ac-DEVD-CHO (a caspase-3 inhibitor) or GSDME small interference RNA (siRNA) were found to attenuate pyroptosis in HGECs. In conclusion, our findings revealed a high expression of caspase-3 and GSDME in the inflamed biopsies of peri-implantitis and confirmed that the caspase-3/GSDME pathway mediates TNF-α-triggered pyroptosis in human gingival epithelium cells, which provides a new target for peri-implantitis treatment.
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http://dx.doi.org/10.1016/j.bbadis.2021.166217DOI Listing
November 2021

Cost-effectiveness of Morse Fall Scale assessment in fall prevention care in hospitalized patients.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 May;46(5):529-535

Department of Nursing, Third Xiangya Hospital, Central South University, Changsha 410013.

Objectives: To analyze the cost-effectiveness of the Morse Fall Scale by clinical big data for nurses in the prevention of falls in hospitalized patients.

Methods: A total of 59 358 hospitalized patients, who came from the Third Xiangya Hospital of Central South University in 1 year before nurses were trained by the Morse Fall Scale, served as a control, including 26 862 males and 32 496 females. While the 66 203 hospitalized patients served as an observation group in 1 year after nurses were trained by the Morse Fall Scale, including 29 881 males and 36 322 females. The time spent by clinical nurses in the fall-risk assessment and the fall number were recorded in the 2 groups, and the relationship was analyzed between the Morse Fall Scale assessment and the clinical ending along with the labor cost of nursing. The cost-effectiveness was analyzed. In addition, the incidence of fall in the observation group was compared between the falling high-risk patients and the non-high-risk patients.

Results: The Morse Fall Scale showed that the incidences of fall in the observation group and the control group were 3.39/100 000 and 3.82/100 000,respectively, there was no significant difference between the 2 groups (=0.807, >0.05); the injury rates of falls of Grade 2 and above in the observation group and the control group were 27.30% and 20.00%, respectively, with no significant difference (χ=0.345, >0.05); but the labor cost was increased by 130 641.82 Chinses Yuan, and the incremental cost-effectiveness ratio was 43 547.27. However, the incidence of fall was significant higher in the falling high-risk patients by the assessment of the Morse Fall Scale than that in the non-high-risk patients (=2.941, <0.05).

Conclusions: The evaluation of the Morse Fall Scale has a certain effect, but it is limited in the prevention of falls in adult hospitalized patients, and the cost-effectiveness analysis is not good. It is recommended to implement the intervention measures for high-risk patients after the assessment, which may improve the management level and efficiency of fall prevention.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200332DOI Listing
May 2021

Genetically modified cell sheets in regenerative medicine and tissue engineering.

Biomaterials 2021 08 24;275:120908. Epub 2021 May 24.

The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, Zhejiang, 310006, China. Electronic address:

Genetically modified cell sheet technology is emerging as a promising biomedical tool to deliver therapeutic genes for regenerative medicine and tissue engineering. Virus-based gene transfection and non-viral gene transfection have been used to fabricate genetically modified cell sheets. Preclinical and clinical studies have shown various beneficial effects of genetically modified cell sheets in the regeneration of bone, periodontal tissue, cartilage and nerves, as well as the amelioration of dental implant osseointegration, myocardial infarction, skeletal muscle ischemia and kidney injury. Furthermore, this technology provides a potential treatment option for various hereditary diseases. However, the method has several limitations, such as safety concerns and difficulties in controlling transgene expression. Therefore, recent studies explored efficient and safe gene transfection methods, prolonged and controllable transgene expression and their potential application in personalized and precision medicine. This review summarizes various types of genetically modified cell sheets, preparation procedures, therapeutic applications and possible improvements.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120908DOI Listing
August 2021

Influence of different incision designs on bone increment of guided bone regeneration (Bio-Gide collagen membrane +Bio-OSS bone powder) during the same period of maxillary anterior tooth implantation.

Bioengineered 2021 12;12(1):2155-2163

Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China.

Exploring the influence of different incision designs on bone increment of guided bone regeneration [Bio-Gide collagen membrane +Bio-OSS bone powder (carbonate apatite crystal extracted from bovine bones), Bio-OSS bone meal was placed on the surface of the bone defect and then covered with a Bio-Gide membrane to close the wound] during the same period of maxillary anterior tooth implantation. The 99 patients from the stomatology department were divided into 3 groups: small incision (N = 30, group A), wide incision (N = 39, group B), internal gingival sulcus incision (N = 30, group C). At the different time (immediately after surgery, 6 months, 12 months and 24 months), the width and height of labial bone at different implant margin (2 mm, 4 mm, 6 mm) has no significant difference in comparison of any two of the three groups (p > 0.05). The score of esthetic feeling in group A was significant higher than group C (P < 0.05). The PPD, the incidence of SH, BOP in group A were all significant higher than group B (P < 0.05). The PISm, PISd, PPD, the incidence of SH and BOP in group A were all significant higher than group C (P < 0.05). The PISm, PISd, PPD, the incidence of SH and BOP in group B were all significant higher than group C (P < 0.05). The three groups has no significant different on the influence bone increment. The soft tissue condition around the implant after surgery was better in internal gingival crevicular incision than others two incisions, large-scale incision better than small incisions.
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http://dx.doi.org/10.1080/21655979.2021.1932209DOI Listing
December 2021

Online dental teaching practices during the COVID-19 pandemic: a cross-sectional online survey from China.

BMC Oral Health 2021 04 12;21(1):189. Epub 2021 Apr 12.

The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, 310006, Zhejiang, China.

Background: Coronavirus disease 2019 (COVID-19) emerged in China in December 2019. The COVID-19 pandemic hindered dental education, as school buildings were closed. Online dental teaching provided an alternative teaching tool for dental education. However, the efficiency of online dental teaching and student preferences for online dental teaching are unclear.

Aim: To investigate the satisfaction with online dental teaching practices among undergraduate dental students and standardized resident physician training students during the COVID-19 pandemic in China.

Methods: A total of 104 undergraduate dental students and 57 standardized resident physician training students from Zhejiang University participated in the study. A 12-item survey was conducted. This investigation included the teaching methods received, frequency of classes, degree of satisfaction, preferred teaching method, whether to participate in a course regarding COVID-19 prevention, and the effects of teaching. The percentages were then calculated and evaluated for each item.

Results: A total of 161 students (104 undergraduate dental students and 57 standardized resident physician training students) participated in this survey. All students had online dental classes during the COVID-19 pandemic. Lecture-based learning (LBL), case-based learning (CBL), problem-based learning (PBL), team-based learning (TBL), and research-based learning (RBL) were selected as teaching methods. Students were more satisfied with LBL and CBL than PBL, RBL, and TBL. The majority of students had more than four classes per week. The most selected protective measures were hand washing, wearing masks, and wearing gloves. A total of 46.6% of students participated in courses on COVID-19. After training, the students consciously chose to wear face shields and protective clothing.

Conclusions: Dental students accepted online dental learning during the COVID-19 pandemic. Students preferred LBL and CBL and were satisfied with the classes. Courses on COVID-19 helped students understand how to prevent COVID-19 transmission in the dental clinic.
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http://dx.doi.org/10.1186/s12903-021-01547-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040365PMC
April 2021

Prevalence of and risk factors associated with sleep disturbances among HPCD exposed to COVID-19 in China.

Sleep Med 2021 04 6;80:16-22. Epub 2021 Jan 6.

Transplantation Center, The Third Xiangya Hospital of Central South University, Changsha, China; The Nursing School of Central South University, Changsha, 410000, China. Electronic address:

Objective: To assess sleep disturbances and associated factors among front-line healthcare providers who have been called upon for, dispatched (HPCD) and exposed to COVID-19 in China.

Methods: This cross-sectional, survey-based, isolation area-stratified study collected demographic data, sleep status and emotional measurements from 1036 HPCD in nine medical institutions from March 5 to 9, 2020 in Wuhan, China, which was the epicenter of the epidemic. HPCD who worked in isolation areas with COVID-19 were eligible for inclusion. The severity of symptoms of sleep disorders, insomnia and emotional self-efficacy were assessed by the Chinese versions of the 10-item Self-rating sleeping situation scale, the seven-item Insomnia Severity Index and the 12-item Regulatory emotional self-efficacy questionnaire, respectively. Univariate analysis was performed to identify factors associated with sleep disturbances. A structural equation model (SEM) was constructed via AMOS to explore the relationship among the four components.

Results: A total of 1036 out of 1075 contacted individuals completed the survey, with a participation rate of 96.4%. A total of 925 (89.3%) were aged 20-39 years, and 755 (72.9%) were women. Among all participants, 874 (84.4%) were nurses, and 162 (15.6%) were physicians; 538 (51.9%) worked in intensive care isolation units; 843 (81.4%) worked in isolation areas for 4 h straight, and 395 (38.1%) perceived COVID-19 peer exposure. A considerable proportion of participants reported symptoms of sleep disorders (543, 52.4%). Exposure status and length of work were the main factors affecting sleep status, which had indirect effects on sleep status by mediating regulatory emotional self-efficacy.

Conclusions: In this survey of HPCD for patients with COVID-19 in China, participants reported experiencing sleep disturbance burdens, especially those having exposure experience and working long shifts. Regulatory emotional self-efficacy (RESE) is an important resource for alleviating sleep disturbances and improving sleep quality. These findings emphasize the importance of being prepared to support HPCD through psychological interventions.
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http://dx.doi.org/10.1016/j.sleep.2020.12.034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834103PMC
April 2021

1α,25-dihydroxyvitamin D3 promotes early osteogenic differentiation of PDLSCs and a 12-year follow-up case of early-onset vitamin D deficiency periodontitis.

J Steroid Biochem Mol Biol 2021 04 21;208:105805. Epub 2021 Jan 21.

The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Yan'an Road, Hangzhou, 310000, Zhejiang Province, China; Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, China. Electronic address:

Periodontitis is a chronic periodontal disease that contributes to tooth loss. In recent years, many animal studies have reported that vitamin D (VitD) deficiency results in chronic periodontitis. However, no studies have reported cases of early-onset periodontitis with VitD deficiency. This study reports a 5-year-old male patient with early-onset periodontitis, VitD deficiency and VitD receptor (VDR) mutation. The patient was treated with VitD and calcium, and received systematic periodontal treatment. During the 12-year treatment, the periodontal conditions of this patient were stable. Our in vitro study found that VitD could promote the expression of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), bone morphogenetic protein 2 (BMP2), bone gamma-carboxyglutamate protein (BGLAP), and VDR in the early osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Meanwhile, VitD could downregulate mRNA expression levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-1β (IL-1β) and protein levels of IL-6 in the tumor necrosis factor-α (TNF-α) -induced inflammation of PDLSCs. Therefore, sufficient VitD supply can be a potential treatment for VitD deficiency induced early-onset periodontitis.
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http://dx.doi.org/10.1016/j.jsbmb.2020.105805DOI Listing
April 2021

Strategically positioning cooperators can facilitate the contagion of cooperation.

Sci Rep 2021 01 13;11(1):1127. Epub 2021 Jan 13.

Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška cesta 160, 2000, Maribor, Slovenia.

The spreading of cooperation in structured population is a challenging problem which can be observed at different scales of social and biological organization. Generally, the problem is studied by evaluating the chances that few initial invading cooperators, randomly appearing in a network, can lead to the spreading of cooperation. In this paper we demonstrate that in many scenarios some cooperators are more influential than others and their initial positions can facilitate the spreading of cooperation. We investigate six different ways to add initial cooperators in a network of cheaters, based on different network-based measurements. Our research reveals that strategically positioning the initial cooperators in a population of cheaters allows to decrease the number of initial cooperators necessary to successfully seed cooperation. The strategic positioning of initial cooperators can also help to shorten the time necessary for the restoration of cooperation. The optimal ways in which the initial cooperators should be placed is, however, non-trivial in that it depends on the degree of competition, the underlying game, and the network structure. Overall, our results show that, in structured populations, few cooperators, well positioned in strategically chosen places, can spread cooperation faster and easier than a large number of cooperators that are placed badly.
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http://dx.doi.org/10.1038/s41598-020-80770-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806618PMC
January 2021

Improved Detonation Performance Via Coordination Substitution: Synthesis and Characterization of Two New Green Energetic Coordination Polymers.

ACS Appl Mater Interfaces 2021 Jan 29;13(1):563-569. Epub 2020 Dec 29.

Key Laboratory of Synthetic & Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China.

In this work, a new energetic coordination polymer (ECP), [Cu(HBTI)(HO)] () (HBTI = 4,5-bistetrazole-imidazole), was synthesized by a hydrothermal method. Due to the existence of coordination water molecules in , however, its energy density was limited, which led to the insufficient detonation performance. To further improve its detonation performance, [Cu(HBTI)(NO)] () was then obtained by substituting the coordinated water molecule in with nitrate through the coordination substitution reaction under acidic conditions. The structures of two ECPs were respectively characterized using X-ray single-crystal diffraction, and the theoretical density of (2.227 g·cm) was greater than (1.851 g·cm). Thermogravimetric analyses showed that has a one-step rapid weight loss process compared with the two-step slow weight loss process of . The theoretical calculations indicated that the detonation performances of were better than those of . Moreover, the promotion effects of two ECPs on the combustion decomposition of ammonium perchlorate were studied using a differential scanning calorimetry method.
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http://dx.doi.org/10.1021/acsami.0c18271DOI Listing
January 2021

Covalent grafting of hyperbranched poly-L-lysine on Ti-based implants achieves dual functions of antibacteria and promoted osteointegration in vivo.

Biomaterials 2021 02 16;269:120534. Epub 2020 Nov 16.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, 310058, China. Electronic address:

The dual functional implants of antibacteria and osteointegration are highly demanded in orthopedic and dentistry, especially for patients who suffer from diabetes or osteoporosis simultaneously. However, there is lack of the facile and robust method to produce clinically applicable implants with this dual function although coatings possessing single function have been extensively developed. Herein, hyperbranched poly-L-lysine (HBPL) polymers were covalently immobilized onto the alkali-heat treated titanium (Ti) substrates and implants by using 3-glycidyloxypropyltrimethoxysilane (GPTMS) as the coupling agent, which displayed excellent antibacterial activity against S. aureus and E. coli with an efficiency as high as 89.4% and 92.2% in vitro, respectively. The HBPL coating also significantly promoted the adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells in vitro. Furthermore, the results of a S. aureus infection rat model in vivo ulteriorly verified that the HBPL-modified screws had good antibacterial and anti-inflammatory abilities at an early stage of implantation and better osteointegration compared with the control Ti screws.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120534DOI Listing
February 2021

Recent advances in light-induced cell sheet technology.

Acta Biomater 2021 01 2;119:30-41. Epub 2020 Nov 2.

The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou 310006, Zhejiang, China; Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, Hangzhou 310006, Zhejiang, China. Electronic address:

Various stimuli have been applied to harvest complete cell sheets, including temperature, magnetic, pH, and electrical stimuli. Cell sheet technology is a convenient and efficient approach with beneficial effects for tissue regeneration and cell therapy. Lights of different wavelengths, such as ultraviolet (UV), visible light, and near infrared ray (NIR) light, were confirmed to aid in fabricating a cell sheet. Changes in the wettability, potential, or water content of the culturing surfaces that occur under light illumination induce conformational changes in the adhesive proteins or collagens, which then leads to cell sheet detachment. However, the current approaches face several limitations, as few standards for safe light illumination have been proposed to date, and require a careful control of the wavelength, power, and irradiation time. Future studies should aim at generating new materials for culturing and releasing cell sheets rapidly and effectively.
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http://dx.doi.org/10.1016/j.actbio.2020.10.044DOI Listing
January 2021

Bi-Layer Shortest-Path Network Interdiction Game for Internet of Things.

Sensors (Basel) 2020 Oct 21;20(20). Epub 2020 Oct 21.

Science and Technology on Information Systems Engineering Laboratory, National University of Defense Technology, Changsha 410073, China.

Network security is a crucial challenge facing Internet-of-Things (IoT) systems worldwide, which leads to serious safety alarms and great economic loss. This paper studies the problem of malicious interdicting network exploitation of IoT systems that are modeled as a bi-layer logical-physical network. In this problem, a virtual attack takes place at the logical layer (the layer of Things), while the physical layer (the layer of Internet) provides concrete support for the attack. In the interdiction problem, the attacker attempts to access a target node on the logical layer with minimal communication cost, but the defender can strategically interdict some key edges on the physical layer given a certain budget of interdiction resources. This setting generalizes the classic single-layer shortest-path network interdiction problem, but brings in nonlinear objective functions, which are notoriously challenging to optimize. We reformulate the model and apply Benders decomposition process to solve this problem. A layer-mapping module is introduced to improve the decomposition algorithm and a random-search process is proposed to accelerate the convergence. Extensive numerical experiments demonstrate the computational efficiency of our methods.
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http://dx.doi.org/10.3390/s20205943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589153PMC
October 2020

Stem-cell-derived ECM sheet-implant complexes for enhancing osseointegration.

Biomater Sci 2020 Dec 19;8(23):6647-6656. Epub 2020 Oct 19.

Department of Implantology, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, No. 395, Yan'an Road, Xia-Cheng Region, Hangzhou, Zhejiang 310006, China.

Numerous treatment methods have been developed to modify the surface of dental implants to improve cell migration and proliferation, removal torque, and osseointegration. Recent studies have constructed cell sheet-implant complexes with enhanced osteogenic capabilities. However, these complexes have some limitations, such as requirements for complex preparation processes, cell vitality maintenance, strict preservation conditions, and the induction of immunogenicity. Extracellular matrix (ECM) sheets without cells may be a more desirable material. To date, the effect of ECM sheets on implant osseointegration has not been reported. In this study, we fabricated ECM sheet-implant complexes through the combination of rat bone marrow mesenchymal stem cell (BMSC)-derived ECM sheets with sandblasted, large-grit, acid-etched (SLA) implants. These complexes were characterized by light microscopy, scanning electron microscopy (SEM), and immunofluorescence (IF) assays. The adhesion, proliferation, and osteogenic differentiation of BMSCs cultured on ECM sheets were detected in vitro. Then, the ECM sheet-implant complexes were transplanted into the metaphysis of the tibias of rats to evaluate the implant osseointegration in vivo. The results showed that ECM sheets were successfully constructed and showed significantly improved adhesion and proliferation. BMSCs cultured on ECM sheets upregulated the expression levels of the osteogenic-related genes alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (Runx2) compared to controls. In vivo, ECM sheet-implant complexes demonstrated superior new bone formation. Our findings proved that the BMSC-derived ECM sheets promoted osseointegration in vitro and in vivo. The current study indicated that the ECM sheet could be an ideal tissue engineering material, and ECM sheet-implant complexes could provide a strategy with low immunogenicity and easy storage and transportation. This research provides a novel strategy for the development of implant surface modification approaches.
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http://dx.doi.org/10.1039/d0bm00980fDOI Listing
December 2020

Activation of Nell-1 in BMSC Sheet Promotes Implant Osseointegration Through Regulating Runx2/Osterix Axis.

Front Cell Dev Biol 2020 22;8:868. Epub 2020 Sep 22.

The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Neural epidermal growth factor-like 1 protein (Nell-1) is first studied because of its association with human craniosynostosis. Nell-1 has been used to accelerate the process of fracture healing because of the osteoinductive ability in recent years. However, the role of Nell-1 during the process of osteointegration is unknown. Here we show that activation of Nell-1 in the BMSC sheet promotes osseointegration and . We found that overexpression of Nell-1 improved osteogenic differentiation and enhanced matrix mineralization of BMSCs through increasing expression of Runx2 and Osterix. Activation of Nell-1 up-regulated the expression ratio of OPG/RANKL, which might have a negative influence on osteoclast differentiation. Furthermore, we obtained BMSC sheet-implant complexes transfected with lentivirus overexpressing and interfering Nell-1 in study, and confirmed that overexpression of Nell-1 promoted new bone formation around the implant and increased the bone-implant contacting area percentage. Our results demonstrate that activation of Nell-1 improves implant osteointegration by regulating Runx2/Osterix axis and shows the potential of BMSC sheet-implant complexes in gene therapy.
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http://dx.doi.org/10.3389/fcell.2020.00868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536315PMC
September 2020

Nursing standard of internet-based rehabilitation for patients with coronavirus disease 2019.

Authors:
Jin Yan Guoli Yang

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2020 May;45(5):513-517

Department of Nursing, Third Xiangya Hospital, Central South University, Changsha 410013, China.

Coronavirus disease 2019 (COVID-19) is an acute respiratory infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Patients with COVID-19 may have respiratory dysfunction, physical dysfunction, and psychological dysfunction. Rehabilitation and long-term follow-up management are particularly important for these patients. Traditional face-to-face rehabilitation possesses high risk of infection, low coverage, time-consuming and laborious. While online rehabilitation nursing mode will be more feasible by using mobile internet technology. Based on literature review and focus group discussion, we standardize the internet-based nursing assessment, plan formulation, implementation, and effectiveness evaluation on discharged patients with COVID-19.We hope it can give guidance for nurses to provide better care for patients.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2020.200338DOI Listing
May 2020

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing.

Mol Ther Methods Clin Dev 2020 Sep 4;18:291-303. Epub 2020 Jun 4.

Department of Implantology, The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.

A robust dento-epithelial junction prevents external pathogenic factors from entering connective tissue and could be crucial for periodontal reattachment after periodontal surgery. The junctional epithelium (JE) is attached to the tooth surface through the hemidesmosome (HD) and internal basal lamina, where the primary component is laminin-332. Destruction of the JE leads to the loss of periodontal attachment. Traditional treatments are effective in eliminating local inflammation of the gingiva; however, few directly promote periodontal reattachment and HD formation. Here, we designed a gene-therapy strategy using the adenovirus-mediated human laminin-332 α3 chain (LAMA3) gene (Ad-LAMA3) transduced into a human-immortalized epidermal cell line (HaCaT) to study the formation of HD . Ad-LAMA3 promoted early adhesion and fast migration of HaCaT cells and increased expression of LAMA3 and type XVII collagen (BP180) significantly. Furthermore, HaCaT cells could facilitate formation of mature HDs after LAMA3 overexpression. experiments demonstrated that the JE transduced with Ad-LAMA3 could increase expression of LAMA3 and BP180 and "biological sealing" between the tooth and gingival epithelium. These results suggested that adenovirus-mediated LAMA3 transduction is a novel therapeutic strategy that promotes the stability and integration of the JE around the tooth during wound healing.
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http://dx.doi.org/10.1016/j.omtm.2020.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334303PMC
September 2020

Surfce Functionalized via AdLAMA3 Multilayer Coating for Re-epithelization Around Titanium Implants.

Front Bioeng Biotechnol 2020 11;8:624. Epub 2020 Jun 11.

The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China.

The peri-implant epithelium (PIE) forms a crucial seal between the oral environment and the implant surface. Compared with the junctional epithelium (JE), the biological sealing of PIE is fragile, which lacks hemidesmosomes (HDs) and internal basal lamina (extracellular matrix containing laminin332, IBL) on the upper part of the interface. In the study, we aim to prepare a coating with good biocompatibility and ability to immobilize the recombinant adenovirus vector of LAMA3 (AdLAMA3) for promoting the re-epithelization of PIE. The titanium surface functionalized with AdLAMA3 was established via layer-by-layer assembly technique and antibody-antigen specific binding. The biological evaluations including cell adhesion and the re-epithelization of PIE were investigated. The results demonstrated that the AdLAMA3 coating could improve epithelial cell attachment and cell spreading in the early stage. experiments indicated that the AdLAMA3 coating on the implant surface has the potential to accelerate the healing of the PIE, and could promote the expression of laminin α3 and the formation of hemidesmosomes. This study might provide a novel approach and experimental evidence for the precise attachment of LAMA3 to titanium surfaces. The process could improve the re-epithelization of PIE.
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http://dx.doi.org/10.3389/fbioe.2020.00624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300264PMC
June 2020

Laminins in osteogenic differentiation and pluripotency maintenance.

Differentiation 2020 Jul - Aug;114:13-19. Epub 2020 May 16.

The Affiliated Stomatology Hospital, Zhejiang University, School of Medicine, Yan'an Road, Hangzhou, China; Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, Yan'an Road, Hangzhou, China. Electronic address:

Extracellular matrix (ECM) plays an integral role in different developmental stages and in multiple systems. However, due to ECM being composed of various extracellular components (growth factors, cytokines, and hormones), its innate complexity and the lack of any efficient purification techniques limit further research into the detailed mechanisms of its role in cellular activities. Laminin (LN), a synthetic recombinant basement membrane protein, can solve the above problems as it is a critical component of ECM and can be completely and reproducibly chemically defined. This article summarizes the functions and mechanisms of LN during osteogenic differentiation and stemness maintenance. LN-111 enhances osteogenic differentiation of mesenchymal stem cells (MSCs) and bone marrow progenitor cells (BMPCs) via the ECM receptor integrin-α1, αV, α6, and β1. LN-332 stimulates osteogenic differentiation of MSCs and bone-marrow-derived MSCs (BMSCs) by α3β1/α3β3 integrin-mediated activation of the focal adhesion kinase (FAK)/extracellular-signal-regulated kinase (ERK), Wnt5a, and bone morphogenic proteins (BMP) signaling pathways. Moreover, LN-111, LN-211, and LN-332 regulate the osteogenic differentiation of dental follicle cells (DFCs) via the integrin-α2/β1 and FAK/ERK signaling pathways. LN-511 and LN-521 can preserve the pluripotency of pluripotent stem cells (PSCs) and human embryonic stem cells (hESCs) via the integrin-α6β1/αVβ1 and the PI3k/Akt pathways. In addition, a variety of laminin fragments such as iMatrix-411; iMatrix-511; bioactive peptide sequences of LN-α2, PPFEGCIWN, and DLTIDDSYWYRI; and LN-332 large globular 3 (LG3), were confirmed to induce osteogenic differentiation. LN511-E8, LN332-E8 fragments, and the laminin-mimicking sequence YIGSR sustain stemness. LN may have potential applications in surface gene markers, xeno-free cultures, and enhancing the expression of osteogenic factors.
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http://dx.doi.org/10.1016/j.diff.2020.05.002DOI Listing
July 2021

Information Cascades and the Collapse of Cooperation.

Sci Rep 2020 05 14;10(1):8004. Epub 2020 May 14.

Department of Computing and Mathematics, Manchester Metropolitan University, Manchester, UK.

In various types of structured communities newcomers choose their interaction partners by selecting a role-model and copying their social networks. Participants in these networks may be cooperators who contribute to the prosperity of the community, or cheaters who do not and simply exploit the cooperators. For newcomers it is beneficial to interact with cooperators but detrimental to interact with cheaters. However, cheaters and cooperators usually cannot be identified unambiguously and newcomers' decisions are often based on a combination of private and public information. We use evolutionary game theory and dynamical networks to demonstrate how the specificity and sensitivity of those decisions can dramatically affect the resilience of cooperation in the community. We show that promiscuous decisions (high sensitivity, low specificity) are advantageous for cooperation when the strength of competition is weak; however, if competition is strong then the best decisions for cooperation are risk-adverse (low sensitivity, high specificity). Opportune decisions based on private and public information can still support cooperation but suffer of the presence of information cascades that damage cooperation, especially in the case of strong competition. Our research sheds light on the way the interplay of specificity and sensitivity in individual decision-making affects the resilience of cooperation in dynamical structured communities.
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http://dx.doi.org/10.1038/s41598-020-64800-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224182PMC
May 2020

Undergraduate nursing students' participation in pre-hospital first aid practice with ambulances in China: A qualitative study.

Nurse Educ Today 2020 Apr 30;90:104459. Epub 2020 Apr 30.

The Third Xiangya Hospital, Central South University, Changsha, China. Electronic address:

Objectives: The aim of this study is to understand the feelings and experiences of nursing undergraduates who participate in pre-hospital first aid and to explore the training programme of pre-hospital first aid for nursing undergraduates. The main objective is to provide a reference for the reform of pre-hospital first aid teaching in undergraduate colleges.

Methods: Semi-structured interviews were conducted with 16 nursing undergraduates who had participated in pre-hospital first aid in an ambulance from a teaching hospital in Hunan, China. The interview data were transcribed, and Colaizzi's seven-step method was used for subject analysis.

Results: The experiences of undergraduate nursing interns can be summarized into five themes: helplessness and fear of death, uncertainty of unknown events, satisfaction with self-improvement, need for professional competence, and the perception of a gap in pre-hospital emergency resources.

Conclusions: Nursing colleges and teaching hospitals should pay attention to death education and pre-hospital first aid training. Teachers should be able to apply situational teaching methods to pre-hospital first-aid teaching and should focus on cultivating the comprehensive abilities of nursing students. At the same time, schools and teaching hospitals should also strengthen the cultivation of professional identity during pre-hospital first aid practice to reduce the rate of nursing personnel turnover. It's very important to supplement and retain professional nurses for the pre-hospital first aid.
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http://dx.doi.org/10.1016/j.nedt.2020.104459DOI Listing
April 2020

HOXA10 inhibit the osteogenic differentiation of periodontal ligament stem cells by regulating β-catenin localization and DKK1 expression.

Connect Tissue Res 2021 07 27;62(4):393-401. Epub 2020 Apr 27.

The Affiliated Stomatology Hospital, Zhejiang University, School of Medicine, Hangzhou, China.

: Human periodontal ligament stem cells (hPDLSCs) are stem cells found near the tooth periodontal ligament. These cels are involved in the regeneration of the periodontal ligament and alveolar bone during orthodontic treatment and chronic periodontitis.: The Homeobox gene HOXA10 regulates the osteogenic differentiation of stem cells. However, the role of HOXA10 in hPDLSCs remains unclear. Therefore, we studied the effects of HOXA10 on human PDLSC osteogenic differentiation .: First, hPDLSCs were isolated and characterized. Second, we assessed the effects of overexpression and knockdown of HOXA10 on PDLSC osteogenic differentiation. Finally, the specific Wnt signaling pathway activator lithium chloride (LiCl) and inhibitor ICG-001 were used to investigate the involvement of the Wnt signaling pathway in HOXA10-induced regulation of osteogenic differentiation.: Overexpressing HOXA10 inhibited PDLSC osteogenic differentiation , shown by ALP and Alizarin Red staining, while HOXA10 knockdown demonstrated the opposite effects. HOXA10 negatively regulated nuclear β-catenin and osteogenic differentiation markers including alkaline phosphatase () and integrin-binding sialoprotein (). Upregulating HOXA10 reduced nuclear β-catenin and increased DKK1 expression. However, HOXA10 knockdown enhanced nuclear β-catenin accumulation and reduced DKK1 expression. These negative effects on osteogenic differentiation by HOXA10 overexpression were restored by the Wnt/β-catenin pathway activator LiCl. The increased osteogenic differentiation effects of HOXA10 knockdown were antagonized by ICG-001, a Wnt pathway inhibitor.: These data demonstrate that HOXA10 inhibits the osteogenic differentiation of periodontal ligament stem cells by regulating β-catenin localization and DKK1.
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http://dx.doi.org/10.1080/03008207.2020.1756271DOI Listing
July 2021

Roles of circular RNAs in regulating the self-renewal and differentiation of adult stem cells.

Differentiation 2020 May - Jun;113:10-18. Epub 2020 Mar 6.

The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China; Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China. Electronic address:

Circular RNAs (circRNAs) are a type of noncoding RNA (ncRNA) and have attracted widespread research attention in recent years. They form a covalently closed loop structure and are widely distributed in tissues and organs. CircRNAs have also been found to regulate a variety of biological processes by functioning as microRNA sponges, transcriptional regulators, and protein binding partners. Recent sequencing results have shown that specific circRNA expression patterns are significantly associated with adult stem cell self-renewal and differentiation, indicating that the differential expression of circRNA affects whether adult stem cells initiate the differentiation process. By studying circRNA expression during adult stem cell differentiation into different lineages, such as the osteogenic, chondrogenic, adipogenic, neurogenic, and cardiac lineages, it can be understood that circRNAs are a potential factor regulating adult stem cell differentiation. Herein, we summarize the general characteristics and functional mechanisms of circRNAs and further examine their roles in adult stem cell self-renewal and differentiation.
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http://dx.doi.org/10.1016/j.diff.2020.03.001DOI Listing
May 2021

Inhibition of osteogenic and adipogenic potential in bone marrow-derived mesenchymal stem cells under osteoporosis.

Biochem Biophys Res Commun 2020 05 11;525(4):902-908. Epub 2020 Mar 11.

Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, PR China. Electronic address:

Osteoporosis, a prevalent systemic bone disease, has emerged as one of the most complicated health issues due to the risk of increased susceptibility to fractures. Bone-marrow mesenchymal stem cell (BMSC) has great potential of differentiating into several distinct cell types, including osteoblasts, adipocytes and chondrocytes. The present study analyzed the biological function changes of BMSCs under osteoporotic micro-environment and aimed to find a specific mechanism associated with this condition. Female rats were assigned to two groups: sham operation (SHAM) group and ovariectomy (OVX) group. BMSCs were harvested and cultured in vitro after 3 months post-ovariectomy. Alamar-Blue test suggested a higher proliferation ability in SHAM group. The differentiation potential of BMSCs was verified through various assays in vitro. RT-PCR and western blot analysis further confirmed the lower osteogenic and adipogenic differentiation capacity in OVX group. Moreover, through the microarray analysis, we were stunned to find that Integrin Alpha-7 (ITGA7) may improve osteogenesis through phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signaling pathway. Overall, our study showed that osteoporosis inhibited the proliferation and differentiation of BMSCs, especially the osteogenesis and adipogenesis. Meanwhile, modulation of ITGA7 expression through PI3K/Akt signaling pathway might provide a new therapeutic target for osteoporosis.
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http://dx.doi.org/10.1016/j.bbrc.2020.03.035DOI Listing
May 2020

Ranking the invasions of cheaters in structured populations.

Sci Rep 2020 02 10;10(1):2231. Epub 2020 Feb 10.

Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška cesta 160, 2000, Maribor, Slovenia.

The identification of the most influential individuals in structured populations is an important research question, with many applications across the social and natural sciences. Here, we study this problem in evolutionary populations on static networks, where invading cheaters can lead to the collapse of cooperation. We propose six strategies to rank the invading cheaters and identify those which mostly facilitate the collapse of cooperation. We demonstrate that the type of successful rankings depend on the selection strength, the underlying game, and the network structure. We show that random ranking has generally little ability to successfully identify invading cheaters, especially for the stag-hunt game in scale-free networks and when the selection strength is strong. The ranking based on degree can successfully identify the most influential invaders when the selection strength is weak, while more structured rankings perform better at strong selection. Scale-free networks and strong selection are generally detrimental to the performance of the random ranking, but they are beneficial for the performance of structured rankings. Our research reveals how to identify the most influential invaders using statistical measures in structured communities, and it demonstrates how their success depends on population structure, selection strength, and on the underlying game dynamics.
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http://dx.doi.org/10.1038/s41598-020-59020-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010670PMC
February 2020

Molecular mechanisms for short root anomaly.

Oral Dis 2021 Mar 13;27(2):142-150. Epub 2020 Jan 13.

The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Short root anomaly (SRA) is a dental disorder that presents an abnormal root morphology with short and blunt dental roots. In this situation, many dental treatments face a difficult challenge, especially orthodontic and prosthodontic treatments. Therefore, an understanding of how SRA develops is urgently needed. Here we describe that the abnormal expression of nuclear factor I C-type (Nfic), osterix (Osx), hedgehog (Hh), bone morphogenetic proteins (BMPs), transforming growth factor-β (TGF-β), Smad, Wnt, β-catenin, and dickkopf-related protein 1 (DKK1) leads to SRA. These factors interact with each other and constitute complicated signaling network in tooth formation. Specifically, BMP signaling inhibits the activity of Wnt/β-catenin directly or by inducing Osx via Runx2-dependent and Runx2-independent pathways. And Osx is a main inhibitor of Wnt/β-catenin signaling. In return, Wnt/β-catenin signaling has an antagonistic action of BMP pathway and a stimulation of Runx2. We highlight the importance of Wnt/β-catenin signaling in the pathological mechanisms. Either suppression or overactivation of this signaling influences the normal odontogenesis. Finally, we list rescue experiments on animal models, which have been reported to restore the interrupted cell differentiation and impaired tooth formation. We hope to find potential treatments for SRA based on these evidences in the future.
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http://dx.doi.org/10.1111/odi.13266DOI Listing
March 2021

Identification of influential invaders in evolutionary populations.

Sci Rep 2019 05 13;9(1):7305. Epub 2019 May 13.

Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška cesta 160, SI-2000, Maribor, Slovenia.

The identification of the most influential nodes has been a vibrant subject of research across the whole of network science. Here we map this problem to structured evolutionary populations, where strategies and the interaction network are both subject to change over time based on social inheritance. We study cooperative communities, which cheaters can invade because they avoid the cost of contributions that are associated with cooperation. The question that we seek to answer is at which nodes cheaters invade most successfully. We propose the weighted degree decomposition to identify and rank the most influential invaders. More specifically, we distinguish two kinds of ranking based on the weighted degree decomposition. We show that a ranking strategy based on negative-weighted degree allows to successfully identify the most influential invaders in the case of weak selection, while a ranking strategy based on positive-weighted degree performs better when the selection is strong. Our research thus reveals how to identify the most influential invaders based on statistical measures in dynamically evolving cooperative communities.
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http://dx.doi.org/10.1038/s41598-019-43853-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6514010PMC
May 2019

The Characteristics and Quality of Mobile Phone Apps Targeted at Men Who Have Sex With Men in China: A Window of Opportunity for Health Information Dissemination?

JMIR Mhealth Uhealth 2019 03 27;7(3):e12573. Epub 2019 Mar 27.

Department of Mathematics and Statistics, Mzuzu University, Mzuzu, Malawi.

Background: A number of mobile phone apps has been developed specifically for men who have sex with men (MSM). We will call these mobile phone apps MSM apps for simplicity. At present, the characteristics and quality and purpose of these MSM apps are unclear.

Objective: The aim of this study was to objectively and comprehensively evaluate the characteristics and quality of the MSM apps to assess whether they disseminated health information among the MSM in China.

Methods: We searched 2 dominant mobile phone app stores (Apple Store and Android Market) to obtain MSM apps using the keywords MSM, gay, lesbian, gay, bisexual, and transgender (LGBT), ,,,,, and . Apps were excluded if they did not have a Chinese language interface or if their target population was not MSM. Basic information about the eligible apps for this study, such as app name, app store category, and date of last update was gathered from the specified app stores. The quality of apps was rated by 2 independent raters using Mobile App Rating Scale (MARS). The intraclass correlation coefficient (ICC) between raters was computed as a measure for interrater reliability of the MARS. All analyses were conducted using SPSS version 20.0 (SPSS Inc).

Results: A total of 575 apps were reviewed between September 15, 2018 and September 30, 2018, out of which, 532 apps were excluded. Finally, 43 apps were included. Of the 43 apps, 16 were from the Apple Store, 10 were from Android Market, and 17 were available in both app stores. In addition, 39 out of 43 apps were for social and sexual networking, whereas 10 contained sexual health information, for example, HIV/sexually transmitted diseases knowledge, HIV test, and condom use. The average rating was 4 stars. The number of downloads for 21 apps exceeded 10,000. A total of 31 apps had acceptable quality (as defined by a MARS score of >3), with functionality as the highest scoring domain, followed by information quality, esthetics, and engagement. Interrater reliability was excellent for the overall mean app quality scores (ICC=.946; 95% CI 0.904-0.970) and the subjective quality scores (ICC=.910; 95% CI 0.841-0.950).

Conclusions: By reviewing the available apps, we found that MSM apps are popular. The majority of MSM apps are for dating, whereas few of them contain HIV prevention and health information. The overall quality of the apps is acceptable. The utilization of mobile phone technologies is a promising way for delivering HIV prevention messages to MSM. We recommend that researchers and app developers should work together to disseminate health information for MSM via mobile technologies.
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http://dx.doi.org/10.2196/12573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456822PMC
March 2019

BMP2-mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis.

J Biomed Mater Res B Appl Biomater 2020 01 25;108(1):80-93. Epub 2019 Mar 25.

Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.

Commercial bone substitute, such as calcined bovine bone (CBB), is currently extensively used as an alternative to autogenous bone. However, CBB lacks osteoinductivity and merely serves as a scaffold for native bone formation. To address this issue, we designed and prepared a heptaglutamate (E7)-modified BMP2-mimicking peptide (7E) and carried out a series of comprehensive physical characterizations and in vivo and in vitro studies to evaluate its role in the repair of cranial defects. The data elucidated that the amount of peptide anchoring to the bone graft materials was remarkably increased after modified with E7. Of note, 7E had a relatively stable and durable release, which promoted the osteogenic differentiation of rat derived bone marrow mesenchymal stem cells (BMSCs) and enhanced the bone regeneration of a rabbit calvarial defect by regulating the expression of the Runx2/SP7 axis. In summary, the composite biomaterials incorporating the E7-modified BMP2-mimicking peptide and CBB prepared in this study is a novel bone augmentation material with the merits of non-immunotoxicity, convenience, and low cost. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:80-93, 2020.
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http://dx.doi.org/10.1002/jbm.b.34368DOI Listing
January 2020

Sustained Release of Antimicrobial Peptide from Self-Assembling Hydrogel Enhanced Osteogenesis.

J Biomater Sci Polym Ed 2018 10 29;29(15):1812-1824. Epub 2018 Sep 29.

c Department of Oral Medicine , Stomatology Hospital, School of Medicine, Zhejiang University , Hangzhou , P. R. China.

Biomaterials have been widely used in bone infection and osteomyelitis resulting from their versatile functionalities. As far as we know, the appearance of osteomyelitis was mainly caused by bacteria. Therefore, a biomaterial that can cure bone infection and promote osteogenesis may become an ideal candidate for the treatment of osteomyelitis. Cationic antimicrobial peptides (AMPs) have been proved to have an excellent ability to kill bacteria, fungi, viruses, and parasites. However, the application of AMPs in bone infection and osteomyelitis is quite limited. Here, we designed a new hydrogel that has an inhibitory effect on the proliferation of S. aureus and enhances osteogenesis. RADA16 self-assembling peptide has been applied for AMPs delivery. In this study, we demonstrated that RADA16 could form a stable structure and afford the sustained release of AMPs. The interwoven nanofiber morphology was detected by field emission scanning electron microscopy. The sustained release study revealed that the release of AMPs could be obtained until 28 days. In vitro research showed this new self-assembling hydrogel could promote the proliferation of bone mesenchymal stem cells (BMSCs) and inhibited the growth of S. aureus. More importantly, the results in vivo also proved that RADA16-AMP self-assembling peptide had an excellent effect on bone formation. Our findings implied that we successfully combined RADA16 and AMPs together and laid the foundation for the application of this new hydrogel and open new avenues for biomaterials.
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http://dx.doi.org/10.1080/09205063.2018.1504191DOI Listing
October 2018
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