Publications by authors named "Guohui Yan"

9 Publications

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MiR-26a-5p Targets WNT5A to Protect Cardiomyocytes from Injury Due to Hypoxia/Reoxygenation Through the Wnt/β-catenin Signaling Pathway.

Int Heart J 2021 Sep 17. Epub 2021 Sep 17.

Department of Medicine, Fujian Medical University.

This study aimed to investigate the effect and mechanism of miR-26a-5p on cardiomyocyte injury induced by hypoxia/reoxygenation (H/R).After construction of an H/R model in rat cardiomyocyte H9c2 cells, miR-26a-5p in the cells was interfered with (cells transfected with miR-26a-5p inhibitor) or overexpressed (cells transfected with a miR-26a-5p mimics). The viability and the apoptosis rate of cells in each group were detected using CCK-8 and flow cytometry; the relationship between miR-26a-5p and WNT5A was verified by a dual-luciferase reporter assay; the expression of miR-26a-5p, WNT5A, cleavedcaspase3 and Wnt/β-catenin signaling pathway-related proteins in each group was detected using qRT-PCR or Western blot; LDH release, SOD, and GSH-PX activities in each group were detected by kit.In the H/R group, the expression level of miR-26a-5p was significantly decreased, whereas the expression level of WNT5A was significantly increased. The activity of the Wnt/β-catenin signaling pathway was up-regulated; the level of LDH released was significantly increased; and activities of SOD and GSH-PX were significantly decreased. The aforementioned changes resulted in decreased cell activity and increased apoptosis rate. The overexpression of miR-26a-5p could reduce the expression level of WNT5A, the activity of the Wnt/β-catenin signaling pathway, and the apoptosis rate and restore the cell viability.These results suggest that miR-26a-5p can target WNT5A and thus, inhibit the Wnt/β-catenin signaling pathway activity, inhibiting H/R-induced cardiomyocyte injury and apoptosis.
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http://dx.doi.org/10.1536/ihj.21-054DOI Listing
September 2021

Measurement of the Brain Volume/Liver Volume Ratio by Three-Dimensional MRI in Appropriate-for-Gestational Age Fetuses and Those With Fetal Growth Restriction.

J Magn Reson Imaging 2021 Jun 21. Epub 2021 Jun 21.

Department of Radiology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Fetal growth restriction (FGR) is associated with a high fetal brain volume/liver volume (FBV/FLV) ratio. Ultrasound may not always be reliable, which has prompted further investigation of MRI techniques.

Purpose: To determine the relationship between FBV/FLV ratio, as measured by MRI, and gestational age (GA) in normal fetuses and those with FGR.

Study Type: Retrospective.

Population: One hundred and forty seven singleton pregnancies including 105 appropriate-for-gestational age (AGA) fetuses and 42 FGR fetuses.

Field Strength/sequence: Three-dimensional fast imaging employing steady-state acquisition at 1.5 T.

Assessment: The FBV and FLV were measured by three radiologists. The inter- and intraobserver agreements, the correlation between FBV/FLV ratio, and advancing GA were evaluated; the diagnostic value of FBV/FLV ratio was evaluated and compared with head circumference/abdominal circumference (HC/AC) ratio measured by ultrasound.

Statistical Tests: Intraclass correlation coefficient (ICC) was used to determine inter- and intraobserver agreements. Regression analysis was used to assess the correlation between FBV/FLV ratio and advancing GA. The diagnostic value of the FBV/FLV ratio was examined by the area under the receiver operating characteristic (ROC) curve.

Results: The inter- and intraobserver agreements were excellent with an interobserver ICC of 0.984 and intra-observer ICCs of 0.989, 0.994, and 0.995. The FBV/FLV ratio in AGA fetuses decreased significantly with advancing GA (Pearson correlation coefficient = -0.844). The FBV/FLV ratio in FGR fetuses was significantly higher than that in AGA fetuses. To identify fetuses at high risk for FGR using the FBV/FLV ratio, the area under the ROC curve was 0.978, with an optimal cut-off value of 4.10. The sensitivity of FBV/FLV ratio in identifying FGR was significantly higher than that of HC/AC ratio (0.929 vs. 0.529).

Data Conclusion: An inverse correlation exists between FBV/FLV ratio and advancing GA in normal fetuses. A high FBV/FLV ratio may be used to ascertain fetuses at high risk for FGR.

Level Of Evidence: 3 Technical Efficacy Stage: 3.
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http://dx.doi.org/10.1002/jmri.27792DOI Listing
June 2021

Diffusion MRI Based Myometrium Tractography for Detection of Placenta Accreta Spectrum Disorder.

J Magn Reson Imaging 2021 Jun 21. Epub 2021 Jun 21.

Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China.

Background: Prenatal diagnosis of placenta accreta spectrum (PAS) disorders is difficult. Magnetic resonance imaging (MRI) has been shown to be a useful supplementary method to ultrasound.

Purpose: To investigate diffusion MRI (dMRI) based tractography as a tool for detecting PAS disorders, and to evaluate its performance compared with anatomical MRI.

Study Type: Prospective.

Population: Forty-seven pregnant women in the third trimester with risk factors for PAS.

Field Strength/sequence: Using fast imaging employing steady-state acquisition and high-angular resolution dMRI at 1.5 Tesla.

Assessment: Diagnosis of PAS was performed by three radiologists based on the dMRI-based feature of myometrial fiber discontinuity and on commonly used anatomical features including presence of dark band, discontinuous myometrium and bladder wall interruption. We evaluated the sensitivity, specificity, accuracy, and area-under-the-curve (AUC) of the individual features and established an integrated model with random forest analysis.

Statistical Tests: Maternal age and gestational age at scan were compared between PAS and control group using a t-test, and childbearing history was compared using a chi-squared test. The random forest model was employed to combine the anatomical and dMRI features with 5-fold cross-validation, and the weight of each feature was normalized to evaluate its importance in predicting PAS.

Results: Based on surgical pathology reports, 16 out of 47 patients had confirmed PAS. The anatomical feature of dark bands and tractography marker achieved the highest AUC of 0.842 for predicting PAS, and the integrated anatomical and tractography features further improved the AUC of 0.880 with an accuracy of 87.2%. The tractography feature contributed most (30.1%) to the integrated model.

Data Conclusion: Myometrial tractography demonstrated superior performance in detecting PAS. Moreover, the combination of dMRI-based tractography and anatomical MRI could potentially improve the diagnosis of PAS disorders in clinical practice.

Level Of Evidence: 2 TECHNICAL EFFICACY STAGE: 2.
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http://dx.doi.org/10.1002/jmri.27794DOI Listing
June 2021

MicroRNA-296-5p inhibits cell proliferation by targeting HMGA1 in colorectal cancer.

Exp Ther Med 2021 Aug 24;22(2):793. Epub 2021 May 24.

The Medical Department of the Xiamen University, Xiamen, Fujian 361000, P.R. China.

An increasing body of evidence indicates the involvement of microRNAs (miRNAs/miRs) in the initiation and progression of colorectal cancer (CRC). miR-296-5p was recently identified as a tumor suppressor in a variety of human cancer types; however, its function in CRC remains largely unknown. The present study demonstrated that the expression of miR-296-5p was significantly downregulated in CRC tissues and cell lines. The overexpression of miR-296-5p markedly inhibited proliferation, and induced cell cycle arrest and apoptosis in CRC cells. Bioinformatics analysis suggested that high mobility group AT-hook 1 (HMGA1) may be a target of miR-296-5p in CRC cells. Further experiments showed that miR-296-5p bound the 3'-untranslated region of HMGA1 and decreased its expression in CRC cells. HMGA1 was overexpressed in CRC tissues and was inversely correlated with the expression of miR-296-5p. The restoration of HMGA1 significantly reversed the inhibitory effect of miR-296-5p on the proliferation of CRC cells. Overall, the findings of the present study indicate that miR-296-5p suppressed the progression of CRC, at least partially via targeting HMGA1. Thus, miR-296-5p is a potential target for novel therapies in CRC.
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http://dx.doi.org/10.3892/etm.2021.10225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170657PMC
August 2021

Mapping fetal brain development based on automated segmentation and 4D brain atlasing.

Brain Struct Funct 2021 Jul 29;226(6):1961-1972. Epub 2021 May 29.

Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.

Fetal brain MRI has become an important tool for in utero assessment of brain development and disorders. However, quantitative analysis of fetal brain MRI remains difficult, partially due to the limited tools for automated preprocessing and the lack of normative brain templates. In this paper, we proposed an automated pipeline for fetal brain extraction, super-resolution reconstruction, and fetal brain atlasing to quantitatively map in utero fetal brain development during mid-to-late gestation in a Chinese population. First, we designed a U-net convolutional neural network for automated fetal brain extraction, which achieved an average accuracy of 97%. We then generated a developing fetal brain atlas, using an iterative linear and nonlinear registration approach. Based on the 4D spatiotemporal atlas, we quantified the morphological development of the fetal brain between 23 and 36 weeks of gestation. The proposed pipeline enabled the fully automated volumetric reconstruction for clinically available fetal brain MRI data, and the 4D fetal brain atlas provided normative templates for the quantitative characterization of fetal brain development, especially in the Chinese population.
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http://dx.doi.org/10.1007/s00429-021-02303-xDOI Listing
July 2021

Prenatal Diagnosis and Classification of Fetal Hypospadias: The Role and Value of Magnetic Resonance Imaging.

J Magn Reson Imaging 2021 06 19;53(6):1862-1870. Epub 2021 Feb 19.

Department of Radiology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Prenatal diagnosis and classification of hypospadias are difficult and of value for management during perinatal and neonatal periods. The conventional approach for prenatal diagnosis of hypospadias is ultrasound; however, this technique may be inconclusive in certain cases, which prompts for further exploration with magnetic resonance imaging (MRI).

Purpose: To investigate the role of MRI in the prenatal diagnosis and classification of fetuses with hypospadias.

Study Type: Retrospective.

Population: Thirty-five fetuses (median gestational age = 37, range 24-39 weeks) with possible hypospadias.

Field Strength/sequence: Single-shot fast spin echo T2-weighted imaging, fast imaging employing steady-state acquisition (FIESTA), and three-dimensional FIESTA acquired at 1.5 T.

Assessment: Diagnosis and classification of hypospadias using MRI were performed by three experienced radiologists based on MRI features, including a short penile shaft, abnormal penile tip, penile curvature, bifid scrotum, "tulip sign," and penoscrotal transposition. The accuracy of MRI in the diagnosis and classification of hypospadias was assessed in comparison to postnatal clinical diagnosis. The interobserver agreement between radiologists was also assessed.

Statistical Tests: Kendall's W test was applied to assess the interobserver agreement between radiologists. Taking postnatal clinical diagnosis as the reference standard, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were calculated.

Results: Of the 35 fetuses, 24 cases were confirmed as hypospadias through postnatal clinical diagnosis. The interobserver agreement between radiologists was substantial (Kendall's W = 0.781, P < 0.001). Of the 24 confirmed cases (13 cases of severe hypospadias and 11 cases of mild hypospadias), 22 cases were correctly diagnosed by MRI. The accuracy of MRI in the diagnosis of hypospadias, severe hypospadias, and mild hypospadias was 85.71%, 82.86%, and 80.00%, respectively.

Data Conclusion: MRI has good performance in the diagnosis of fetal hypospadias. In addition, MRI could help evaluate the severity of fetal hypospadias.

Level Of Evidence: 3 TECHNICAL EFFICACY: Stage 2.
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http://dx.doi.org/10.1002/jmri.27519DOI Listing
June 2021

Effect of using diode laser on Enterococcus faecalis and its lipoteichoic acid (LTA) in chronic apical periodontitis.

Lasers Med Sci 2021 Jul 23;36(5):1059-1066. Epub 2020 Sep 23.

Department of Endodontics, Tianjin Medical University Stomatology Hospital, Building Number 12, Qixiangtai Road, Heping District, Tianjin, 300070, China.

The purpose of this study was to evaluate the effect of diode laser irradiation on Enterococcus faecalis (E. faecalis) and its lipoteichoic acid (LTA). Ninety-six freshly extracted single-rooted teeth were divided into six groups, n = 8 per group. Groups 1, 2, 3, and 4 as laser group (810 nm PILOT™ Diode Laser, 400 μm fiber diameter, continuous mode, 30 s time) with powers at 1.0 W, 1.5 W, 2.0 W, and 2.5 W respectively. Group 5 or positive control group (3 ml of 1% sodium hypochlorite (NaOCl) irrigation) and group 6 or negative control group (3 ml of normal saline (0.9% NaCl) irrigation). Root canal samples were collected before and after receiving laser irradiation and irrigation solution. Cultivable bacteria were determined by counting the colony (CFU/ml). Evaluation of temperature on the external root surface of teeth was done with K type thermocouple using laser at different powers. Enzyme-linked immunosorbant assay (ELISA) was performed to measure the LTA levels and the correlations between E. faecalis count, LTA levels, and rise in temperature were observed using Pearson's correlation test. E. faecalis LTA was subjected to laser irradiation and its structural damage was examined by thin layer chromatography (TLC). Compared with the control groups, all laser groups showed a decreased colony counts and decreased LTA levels with statistically significant difference (p ˂ 0.05). The bactericidal effect and LTA reduction of laser was better at 2.5 W power. Laser at 2.5 W power had temperature rise of more than 7 °C which is beyond the safe thermal threshold level. No statistically significant correlation was found between E. faecalis count, levels of LTA, and rise in external root surface temperature (p ˃ 0.05). TLC results showed a structural damage in the glycolipid moiety of E. faecalis LTA. Diode laser can effectively reduce the E. faecalis count and its LTA levels.
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http://dx.doi.org/10.1007/s10103-020-03146-4DOI Listing
July 2021

Fetal brain age estimation and anomaly detection using attention-based deep ensembles with uncertainty.

Neuroimage 2020 12 2;223:117316. Epub 2020 Sep 2.

Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, 310027, China. Electronic address:

MRI-based brain age prediction has been widely used to characterize normal brain development, and deviations from the typical developmental trajectory are indications of brain abnormalities. Age prediction of the fetal brain remains unexplored, although it can be of broad interest to prenatal examination given the limited diagnostic tools available for assessment of the fetal brain. In this work, we built an attention-based deep residual network based on routine clinical T2-weighted MR images of 659 fetal brains, which achieved an overall mean absolute error of 0.767 weeks and R of 0.920 in fetal brain age prediction. The predictive uncertainty and estimation confidence were simultaneously quantified from the network as markers for detecting fetal brain anomalies using an ensemble method. The novel markers overcame the limitations in conventional brain age estimation and demonstrated promising diagnostic power in differentiating several types of fetal abnormalities, including small head circumference, malformations and ventriculomegaly with the area under the curve of 0.90, 0.90 and 0.67, respectively. In addition, attention maps were derived from the network, which revealed regional features that contributed to fetal age estimation at each gestational stage. The proposed attention-based deep ensembles demonstrated superior performance in fetal brain age estimation and fetal anomaly detection, which has the potential to be translated to prenatal diagnosis in clinical practice.
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http://dx.doi.org/10.1016/j.neuroimage.2020.117316DOI Listing
December 2020

Exposure to diode laser (810nm) affects the bacterial adherence and biofilm formation in a E. faecalis biofilm model.

Photodiagnosis Photodyn Ther 2020 Sep 30;31:101772. Epub 2020 May 30.

School of Stomatology, Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin, 300070, China.

Background: A large number of studies have shown that diode laser can effectively kill Enterococcus faecalis (E. faecalis). However, to our knowledge there has been little information regarding high-level analysis of sterilization mechanism on E. faecalis biofilm models after laser irradiation. This study provides emphasis on the E. faecalis viability and exopolysaccharide content after laser irradiation. It also aims to examine whether diode laser affects the biofilm formation and adherence of E. faecalis biofilm at the level of gene expression.

Methods: E. faecalis (ATCC 29,212) was inoculated for biofilm formation. After growing for 48 h, the biofilms were submitted to the following treatments, twice daily (n = 6): (i) Diode laser with energy densities as 28.7 J/cm; (ii) 1% sodium hypochlorite (NaOCl) was used as a positive control; (iii) 0.9 % physiological saline (NaCl) was used as a negative control. After 120 h of biofilm growth, bactericidal activity of diode laser was evaluated by using plate count method and scanning electron microscopy (SEM). The distribution of extracellular polysaccharide was assessed by Confocal laser scanning microscopy (CLSM). Anthrone method was used to quantify the amount of water-soluble exopolysaccharide (WSE) and water-insoluble exopolysaccharides (WIE) in E. faecalis. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to assess transcription of genes (gelE, ace and esp) related to formation and adherence of E. faecalis biofilm.

Results: Compared with NaCl, diode laser significantly destabilizedE. faecalis biofilm, which showing a decrease in the number of bacteria and inhibiting the adherence of biofilm (p < 0.05). From Anthrone method and CLSM, the study found that diode laser lowered the concentration of WSE and WIE in biofilms (p < 0.05). In addition, transcription of gene gelE, ace and esp were also reduced after irradiation of diode laser (p < 0.05).

Conclusion: By inhibiting the synthesis of WIE and WSE, diode laser may reduce the formation of E. faecalis biofilm. The expression of specific genes (gelE, ace and esp) involved in bacterial adherence and biofilm formation were down regulated under the exposure to diode laser (810 nm), which in turn is expected to decrease the pathogenicity of E. faecalis.
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http://dx.doi.org/10.1016/j.pdpdt.2020.101772DOI Listing
September 2020
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