Publications by authors named "Guohao Li"

22 Publications

  • Page 1 of 1

Variations in characteristics and transport pathways of PM during heavy pollution episodes in 2013-2019 in Jinan, a central city in the north China Plain.

Environ Pollut 2021 May 24;284:117450. Epub 2021 May 24.

School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China.

The characteristics and transport pathways of air masses vary during heavy pollution episodes (HPEs). Three categories of HPEs have been defined: HPE Ι, II, and III, corresponding to HPE durations of 1, 2, and at least 3 days, respectively. Sixty HPEs were investigated in this study. The number of HPEs decreased from 2013 to 2017 and then increased from 2017 to 2019, dominated by emission reductions and meteorological conditions. The average and maximum PM (i.e., aerodynamic diameter of <2.5 μm) concentrations during those HPEs in 2019 decreased by 5.6%-11.8% and 11.9%-38.5%, respectively, compared with those in 2013. The longer the duration of an HPE, the higher the PM concentration. Secondary inorganic aerosol concentrations and their contents in PM during HPE Ⅲ were found to be higher than those during HPEs Ι and Ⅱ, as secondary transformations of precursor gases are more intense during long-term HPEs. The dominant trajectories of airflow arriving in Jinan originated from the southern and southeastern regions during HPEs, realized using the Hybrid Single Particle Lagrangian Integrated Trajectory. The trajectories from the north and west of Jinan contained the highest PM concentrations of 323.3-432.1 μg/m during HPE Ⅲ, although these trajectories only contributed 5.6%-11.1% of the total dominant transport pathways, while those in trajectories from the northwest were highest during HPEs Ι and Ⅱ. The highest contributions of air masses from short distances were found during HPE Ⅲ, of 77.8%, while they were only 65.6% and 47.8% during HPEs Ι and II, respectively. More attention should be given to transport pathways within the short distance from Jinan. Therefore, enhancing regional cooperation in Jinan and surrounding regions (particularly in the south, southeast, northwest, west, and north) is critical for improving air quality in the North China Plain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.117450DOI Listing
May 2021

DeepGCNs: Making GCNs Go as Deep as CNNs.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 19;PP. Epub 2021 Apr 19.

Convolutional Neural Networks have been very successful at solving a variety of computer vision tasks such as object classification and detection, semantic segmentation, activity understanding, to name just a few. One key enabling factor for their great performance has been the ability to train very deep networks. Despite their huge success in many tasks, CNNs do not work well with non-Euclidean data, which is prevalent in many real-world applications. Graph Convolutional Networks offer an alternative that allows for non-Eucledian data input to a neural network. While GCNs already achieve encouraging results, they are currently limited to architectures with a relatively small number of layers, primarily due to vanishing gradients during training. This work transfers concepts such as residual/dense connections and dilated convolutions from CNNs to GCNs in order to successfully train very deep GCNs. We show the benefit of using deep GCNs experimentally across various datasets and tasks. Specifically, we achieve promising performance in part segmentation and semantic segmentation on point clouds and in node classification of protein functions across biological protein-protein-interaction graphs. We believe that the insights in this work will open avenues for future research on GCNs and their application to further tasks not explored in this paper.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2021.3074057DOI Listing
April 2021

Cell-Released Magnetic Vesicles Capturing Metabolic Labeled Rare Circulating Tumor Cells Based on Bioorthogonal Chemistry.

Small 2021 May 21;17(18):e2007796. Epub 2021 Mar 21.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, P. R. China.

Capture of circulating tumor cells (CTCs) with high efficiency and high purity holds great value for potential clinical applications. Besides the existing problems of contamination from blood cells and plasma proteins, unknown/down-regulated expression of targeting markers (e.g., antigen, receptor, etc.) of CTCs have questioned the reliability and general applicability of current CTCs capture methodologies based on immune/aptamer-affinity. Herein, a cell-engineered strategy is designed to break down such barriers by employing the cell metabolism as the leading force to solve key problems. Generally, through an extracellular vesicle generation way, the cell-released magnetic vesicles inherited parent cellular membrane characteristics are produced, and then functionalized with dibenzoazacyclooctyne to target and isolate the metabolic labeled rare CTCs. This strategy offers good reliability and broader possibilities to capture different types of tumor cells, as proven by the capture efficiency above 84% and 82% for A549 and HepG2 cell lines as well as an extremely low detection limitation of 5 cells. Moreover, it enabled high purity enrichment of CTCs from 1 mL blood samples of tumor-bearing mice, only ≈5-757 white blood cells are non-specific caught, ignoring the potential phenotypic fluctuation associated with the cancer progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202007796DOI Listing
May 2021

Emission factors and characteristics of volatile organic compounds (VOCs) from adhesive application in indoor decoration in China.

Sci Total Environ 2021 Jul 20;779:145169. Epub 2021 Feb 20.

Beijing Key Laboratory for VOCs Pollution Prevention and Treatment Technology and Application of Urban Air, National Engineering Research Center of Urban Environmental Pollution Control, Beijing Municipal Research Institute of Environmental Protection, Beijing 100037, China. Electronic address:

Adhesive application in indoor decoration is an important anthropogenic volatile organic compound (VOC) emission source of both indoors and outdoors. However, few studies have been conducted on VOC emission factors and characteristics from indoor decorating adhesives. In this study, the VOC emission factors were obtained by measurement of VOCs in 210 adhesives. The results showed that the VOC emission factors were 41.23 g/L for wall and ground solidify, 33.49 g/L for tile adhesive, 76.88 g/L for white glue, 52.36 g/L for wallcovering adhesive, 132.28 g/L for sealant glue, 49.33 g/kg for foaming adhesive, 654.23 g/L for all-purpose adhesive, 251.93 g/L for free nails adhesive, 152.01 g/L for marble glue, and 136.79 g/L for beautiful sealant. Methodology for calculating activity data of decorating adhesive consumptions was developed and a VOC emission inventory from adhesive application in indoor decoration was developed using a bottom-up estimation methodology. The VOC emissions from 2012 to 2017 in China were 235,987.76, 246,230.47, 250,981.62, 249,849.48, 227,150.33 and 212, 433.07 t, respectively. The beautiful sealant, wall and ground solidify, sealant glue and all-purpose adhesive contributed the most of the total emissions, collectively accounting for 78.14%. Shandong, Jiangsu, Zhejiang, Sichuan and Guangdong ranked as the top five provinces for VOC emissions, together contributing 39.10% to the national total emissions. Shandong and Jiangsu reached up to 17,057.95 t/year and 15,207.92 t/year, respectively. Priority should be given to four types of adhesives with pretty high VOC contents for designing effective VOC control measures, including solvent-based all-purpose adhesive, solvent-based free nails adhesive, solvent-based sealant glue, and solvent-based beautiful sealant. Future emission trends are projected through 2030 based on current emission control policies and real estate trend. It may be possible to reduce VOC emissions by 60.81% and 69.37% by 2030 under the two scenarios, respectively, compared with the VOC emissions in 2017.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.145169DOI Listing
July 2021

A magnetic surface-enhanced Raman scattering platform for performing successive breast cancer exosome isolation and analysis.

J Mater Chem B 2021 03 8;9(11):2709-2716. Epub 2021 Mar 8.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

The rapid development of exosome research provides new insights into the physiological role of exosomes and their significant correlation with human health. Although the exosomes derived from tumor sources have been proven to be promising biomarkers for cancer detection and disease progression due to their inherited biological contents from the parent cancer cells and unique roles in tumor metastasis and invasion, it is still a challenging task to perform rapid and effective isolation from complex biological samples and conduct high-precision real-time analysis. Herein, we propose a magnetic surface-enhanced Raman scattering (SERS) platform to integrate successive breast cancer exosome isolation and Raman signal enhancement into one system to achieve the goal. In addition, principal component analysis (PCA) was conducted to investigate major patterns of the samples. According to the results, the magnetic SERS platform can be applied to distinguish exosomes derived from MCF-7 and MDA-MB-231 cells with 100% sensitivity and 100% specificity for the 95% confidence interval. More importantly, this platform can fully identify breast cancer patients and healthy people with 91.67% sensitivity and 100% specificity. These studies revealed that our magnetic SERS platform would serve as a great potential system for highly efficient real-time liquid biopsy by using the exosomes as cancerous markers, while exempting from pre-treatment of clinical samples or the extra introduction of elements for SERS signal enhancement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0tb02894kDOI Listing
March 2021

A light-up fluorescence resonance energy transfer magnetic aptamer-sensor for ultra-sensitive lung cancer exosome detection.

J Mater Chem B 2021 03 3;9(10):2483-2493. Epub 2021 Mar 3.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

In vitro liquid biopsy based on exosomes offers promising opportunities for fast and reliable detection of lung cancers. In this work, we present a fluorescence resonance energy transfer (FRET) magnetic aptamer-sensor for magnetic enrichment of exosomes with aptamers and detection of cancerous-surface proteins based on a light-up FRET strategy. Fluorescent quantum dots (QDs) and aptamers were introduced onto magnetic nanoparticles and the fluorescence emission turned down when the aptamers were paired with their complementary DNA on the surface of Au nanoparticles. Later, competitive binding of exosomes with the aptamers expelled the Au nanoparticles resulting in an exosome concentration-dependent linear increase of QD fluorescence intensity in a broad exosome concentration range (5 × 10-5 × 10 particles per mL). As found in our work, this system behaved ultra-sensitively and the calculated detection limit of this FRET magnetic aptamer-sensor was as low as 13 particles per mL. Furthermore, taking epithelial cancer-specific antigen (epithelial cell adhesion molecule, EpCAM) screening as a typical example, our built FRET magnetic aptamer-sensor allowed a rapid and efficient distinction of all the epithelial cancer cases (7 lung cancers and 5 other cancers) from health volunteers with 100% accuracy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1tb00046bDOI Listing
March 2021

Multistage Nanoparticle Delivery System-A New Approach to Cancer Therapeutics.

J Biomed Nanotechnol 2020 Nov;16(11):1570-1587

Traditional methods of tumor therapy have many limitations. Thus, cancer nanomedicine is developing rapidly as a new treatment for tumors. In the past decade, the literature in this field has almost doubled every two years. However, the therapeutic nanoparticle (NP) platforms that have been approved for cancer treatment have not achieved the expected results in clinical application. Cancer nanomedicine still faces many obstacles and challenges. An abnormal cancer vascular system and a thick interstitial matrix can impose physiological barriers. As a result, drug delivery in tumor tissue depends mainly on diffusion. The diffusion efficiency of large NPs is poor; they are trapped around the blood vessels. Smallmolecule drug conjugates (SMDCs), miniaturized biologic drug conjugates (mBDCs), and small NPs can pass through this barrier. However, poor aggregation in tumors, easy elimination, and poor pharmacokinetics (PK) limit their therapeutic effects. In recent years, a selective new multistage delivery system was proposed to solve the challenge of infiltration. By incorporating smaller NPs or molecular drugs into large controlled-release particles for multistep delivery to tumors, we can make full use of the advantageous pharmacokinetics, the accumulation of large particles in the tumor, and the deep infiltration of small particles. In addition to changing the particle size, the multistage NP delivery system can also change the shape, charge, flexibility, and surface coating of the NPs to enhance penetration. Based on recent multistage delivery system research, this review expounds on the main direction of multistage delivery considering the ways in which large particles are triggered to release small particles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1166/jbn.2020.2996DOI Listing
November 2020

Theaflavin ameliorates renal ischemia/reperfusion injury by activating the Nrf2 signalling pathway in vivo and in vitro.

Biomed Pharmacother 2021 Feb 16;134:111097. Epub 2020 Dec 16.

Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Studies have demonstrated that oxidaive stress-induced apoptosis may be the main pathogenic mechanism of renal ischemia/reperfusion (I/R) injury. Theaflavin, a polyphenolic compound extracted from black tea, has been proven to exert strong antioxidant biological function. The objective of the present study was to investigate the potential role of theaflavin on renal I/R injury and its potential molecular mechanism both in vitro and in vivo. C57/BL6 J mice were used to create a model of I/R injury wherein mice were ligated with bilateral renal pedicles for 45 min, and then reperfused for 24 h. A hypoxia/reoxygenation (H/R) model of TCMK-1 cells was used to simulate I/R in vitro. Theaflavin were administered to the treatment group first and then established the model. Kidney Injury Molecule-1 (KIM-1), serum creatinine, urea nitrogen, and 24-h urinary protein levels were evaluated and changes in mitochondrial membrane potential and the ultrastructure of mitochondria were observed. Cell viability, oxidative stress damage, and apoptosis were assessed. The expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream target genes HO-1 and NQO1 were evaluated. Our results revealed that pretreatment with theaflavin significantly inhibited I/R- and H/R-induced renal injury and cell apoptosis. Theaflavin improved mitochondrial dysfunction by attenuating mitochondrial damage and promoting mitochondrial membrane potential. Theaflavin pretreatment significantly reduced malondialdehyde content, while enhancing superoxide dismutase activity in vivo and in vitro. It also reduced oxidative stress and apoptosis mainly by upregulating Nrf2 and its downstream targets in TCMK-1 cells. Thus, theaflavin exerted a protective effect against renal I/R injury by inhibiting oxidative stress and apoptosis via activation of the Nrf2-NQO1/HO-1 pathway as well as correcting mitochondrial dysfunction, thereby presenting its potential as a clinical therapeutic in cases of acute kidney injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2020.111097DOI Listing
February 2021

Air pollution episodes during the COVID-19 outbreak in the Beijing-Tianjin-Hebei region of China: An insight into the transport pathways and source distribution.

Environ Pollut 2020 Dec 8;267:115617. Epub 2020 Sep 8.

Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing, 100124, China.

Although anthropogenic emissions decreased, polluted days still occurred in the Beijing-Tianjin-Hebei (BTH) region during the initial outbreak of the coronavirus disease (COVID-19). Analysis of the characteristics and source distribution of large-scale air pollution episodes during the COVID-19 outbreak (from 23 January to April 8, 2020) in the BTH region is helpful for exploring the efficacy of control measures and policy making. The results indicated that the BTH region suffered two large-scale air pollution episodes (23-28 January and 8-13 February), which were characterized by elevated PM, SO, NO, and CO concentrations, while the O concentration decreased by 1.5%-33.9% (except in Shijiazhuang, where it increased by 16.6% during the second episode). These large-scale air pollution episodes were dominated by unfavorable meteorological conditions comprising a low wind speed and increased relative humidity. The transport pathways and source distribution were explored using the Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT), potential source contribution function (PSCF), and concentration weighted trajectory (CWT) models. The air pollution in the BTH region was mainly affected by local emission sources during the first episode, which contributed 51.6%-60.6% of the total trajectories in the BTH region with a PM concentration ranging from 146.2 μg/m to 196.7 μg/m. The short-distance air masses from the southern and southwestern areas of the BTH region were the main transport pathways of airflow arriving in the BTH region during the second episode. These contributed 51.9%-57.9% of the total trajectories and originated in Hebei, Henan, central Shanxi, and Shaanxi provinces, which were the areas contributing the most to the PM level and exhibited the highest PSCF and CWT values. Therefore, on the basis of local emission reduction, enhancing regional environmental cooperation and implementing a united prevention and control of air pollution are effective mitigation measures for the BTH region.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2020.115617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477629PMC
December 2020

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.

Aging (Albany NY) 2020 11 16;13(2):1842-1858. Epub 2020 Nov 16.

Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

We investigated the prognostic significance of Death-Associated Protein Kinase 1 (DAPK1) and its role in sunitinib resistance in clear cell renal cell carcinoma (ccRCC). DAPK1 mRNA levels were significantly lower in tumor tissues than normal kidney tissues in TCGA-KIRC dataset (n=428). Both overall survival and disease-free survival were significantly shorter in ccRCC patients with low DAPK1 expression than those with high DAPK1 expression. Receiver operating characteristic curve analysis showed that low DAPK1 expression correlated with poor prognosis in ccRCC patients. Multivariate analysis confirmed that DAPK1 expression was an independent prognostic indicator in ccRCC. Gene set enrichment analysis showed that low DAPK1 expression correlates with upregulation of pathways related to metastasis, drug resistance, hypoxia and invasiveness in ccRCC patients. Sunitinib-resistant ccRCC cells show significantly lower DAPK1 mRNA and protein levels than sunitinib-sensitive ccRCC cells. DAPK1 overexpression enhances apoptosis in sunitinib-resistant ccRCC cells via the ATF6-dependent ER stress pathway. Xenograft tumors derived from DAPK1-overxpressing ccRCC cells were significantly smaller than the controls in nude mice. Our finding demonstrates that low DAPK1 expression is an independent prognostic indicator that correlates with ccRCC progression and sunitinib resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.103638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880360PMC
November 2020

Bottlebrush-like highly efficient antibacterial coating constructed using α-helical peptide dendritic polymers on the poly(styrene--(ethylene--butylene)--styrene) surface.

J Mater Chem B 2020 08;8(33):7428-7437

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

Infectious diseases induced by pathogenic bacteria are the major causes for the failure of medical implants. Meanwhile, the drug-resistance is steadily developed because of the large and even inappropriate use of antibiotics. Therefore, the development of antibacterial coating with non-antibiotic-based agents on the surfaces of medical implants and devices has been an urgent need. Herein, we propose a bottlebrush-like antibacterial coating on a poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) triblock copolymer surface by UV-induced graft polymerization of poly(ethylene glycol) (PEG) acrylate terminated poly(lysine dendrimer). This PEG-conjugated antibacterial polymer possessed a substructure of α-helical backbone and cation dendrimer side chains stretching in the radial directions of the helix. The introduction of lysine peptide dendrimers endowed the prepared antibacterial polymer with precisely controlled characteristics of its local cation density, amphipathic composition as well as three-dimensional (3D) conformation to improve interactions with bacterial membranes. The antimicrobial assay and biocompatibility assay results showed that 96.83% of S. aureus and 99.99% of E. coli were killed after being in contact with the antibacterial coating, while no toxicity to mammalian cells or no hemolysis was detected. This antimicrobial activity was further confirmed by the molecular dynamics simulation results, which demonstrated that the employment of lysine peptide dendrimers enhanced the electrostatic interaction and hydrogen bonding between the brush and bacterial membranes remarkably. Such bottlebrush-like antibacterial coating constructed using α-helical peptide dendritic polymers may become an effective strategy for manufacturing antibacterial products for biomedical uses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0tb01336fDOI Listing
August 2020

A-kinase interacting protein 1, a potential biomarker associated with advanced tumor features and CXCL1/2 in prostate cancer.

Int J Biol Markers 2020 Jun 27;35(2):74-81. Epub 2020 Apr 27.

Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.

Objective: This study aimed to investigate the correlation of A-kinase interacting protein 1 (AKIP1) with chemokine (C-X-C motif) ligand 1 (CXCL1) and CXCL2, as well as their associations with clinical characteristics and prognosis in prostate cancer patients.

Methods: A total of 248 eligible prostate cancer patients who underwent surgery were consecutively recruited, and tumor tissues were collected during the surgery. AKIP1, CXCL1, and CXCL2 expression in tumor tissues were assessed by immunohistochemistry. Disease-free survival and overall survival were recorded, and the median follow-up time was 27 months.

Results: The proportion of patients with AKIP1, CXCL1, and CXCL2 high expression was 56.5%, 63.7%, and 56.9%, respectively. Additionally, AKIP1 expression positively correlated with CXCL1 expression (<0.001) and CXCL2 expression (<0.001), and CXCL1 expression was positively associated with CXCL2 expression (<0.001). Furthermore, AKIP1 expression positively correlated with pathological T stage (<0.001) and pathological N stage (=0.003). CXCL1 expression was positively associated with pathological T stage (<0.001) and pathological N stage (<0.001) as well. However, the CXCL2 expression only positively correlated with pathological T stage (=0.002). Also, AKIP1 high expression correlated with worse disease-free survival (=0.049) and OS (=0.013), and CXCL1 high expression was associated with unfavorable disease-free survival (=0.023) but not overall survival (=0.052). CXCL2 expression was not correlated with disease-free survival (=0.083) or overall survival (=0.065). Multivariate Cox's regression disclosed that AKIP1 high expression independently predicted worse overall survival (=0.009).

Conclusion: AKIP1 positively associates with CXCL1/2 and is a potential biomarker for disease monitoring as well as prognosis in prostate cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1724600820914944DOI Listing
June 2020

A 17-Gene Signature Predicted Prognosis in Renal Cell Carcinoma.

Dis Markers 2020 26;2020:8352809. Epub 2020 Feb 26.

Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.

Renal cell carcinoma (RCC), which was one of the most common malignant tumors in urinary system, had gradually increased incidence and mortality in recent years. Although significant advances had been made in molecular and biology research on the pathogenesis of RCC, effective treatments and prognostic indicators were still lacking. In order to predict the prognosis of RCC better, we identified 17 genes that were associated with the overall survival (OS) of RCC patients from The Cancer Genome Atlas (TCGA) dataset and a 17-gene signature was developed. Through SurvExpress, we analyzed the expression differences of the 17 genes and their correlation with the survival of RCC patients in five datasets (ZHAO, TCGA, KIPAN, KIRC, and KIRP), and then evaluated the survival prognostic significance of the 17-gene signature for RCC. Our results showed that the 17-gene signature had a predictive prognostic value not only in single pathologic RCC, but also in multiple pathologic types of RCC. In conclusion, the 17-gene signature model was related to the survival of RCC patients and could help predict the prognosis with significant clinical implications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/8352809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063218PMC
October 2020

Efficacy of bladder perfusion of alternating hydroxycamptothecin and gemcitabine combined with low-dose tuberculin in the treatment of non-muscle invasive bladder cancer after TURBT.

J BUON 2019 Jul-Aug;24(4):1652-1658

Department of Urology, Central Hospital of Wuhan, Tonji Medical College, Huazhong University of Science and Technology, Wuhan, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
April 2020

Inhibition of miR-9-5p suppresses prostate cancer progress by targeting StarD13.

Cell Mol Biol Lett 2019 8;24:20. Epub 2019 Mar 8.

Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, No. 26 Shengli Street, Jiang'an District, Wuhan, 430014 China.

Background: This study aims to investigate the effects of inhibiting microRNA-9-5p (miR-9-5p) on the expression of StAR-related lipid transfer domain containing 13 (StarD13) and the progress of prostate cancer.

Methods: The mRNA expression levels of miR-9-5p and StarD13 were determined in several prostate cancer cell lines. We chose DU145 and PC-3 cells for further research. The CCK8 assay was used to measure the cell viability. The cell invasion and wound-healing assays were respectively applied to evaluate invasion and migration. The expression of E-cadherin (E-cad), N-cadherin (N-cad) and vimentin were measured via western blot. DU145 and PC-3 cells overexpressing StarD13 were generated to investigate the variation in proliferation, invasion and migration. A luciferase reporter assay was used to identify the target of miR-9-5p.

Results: Our results show that miR-9-5p was highly expressed and StarD13 was suppressed in prostate cancer cells. MiR-9-5p inhibition repressed the cells' viability, invasion and migration. It also increased the expression of E-cad and decreased that of N-cad and vimentin. StarD13 overexpression gave the same results as silencing of miR-9-5p: suppression of cell proliferation, invasion and migration. The bioinformatics analysis predicted StarD13 as a target gene of miR-9-5p. Quantitative RT-PCR, western blot analysis and the dual-luciferase reporter assay were employed to confirm the prediction.

Conclusion: Our results show that miR-9-5p plays a powerful role in the growth, invasion, migration and epithelial-mesenchymal transition (EMT) of prostate cancer cells by regulating StarD13. A therapeutic agent inhibiting miR-9-5p could act as a tumor suppressor for prostate cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s11658-019-0145-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408831PMC
April 2019

1-Pyrroline-5-carboxylate released by prostate Cancer cell inhibit T cell proliferation and function by targeting SHP1/cytochrome c oxidoreductase/ROS Axis.

J Immunother Cancer 2018 12 13;6(1):148. Epub 2018 Dec 13.

Department of Urology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Tumor cell mediated immune-suppression remains a question of interest in tumor biology. In this study, we focused on the metabolites that are released by prostate cancer cells (PCC), which could potentially attenuate T cell immunity.

Methods: Prostate cancer cells (PCC) media (PCM) was used to treat T cells, and its impact on T cell signaling was evaluated. The molecular mechanism was further verified in vivo using mouse models. The clinical significance was determined using IHC in human clinical specimens. Liquid chromatography mass spectroscopy (LC/MS-MS) was used to identify the metabolites that are released by PCC, which trigger T cells inactivation.

Results: PCM inhibits T cells proliferation and impairs their ability to produce inflammatory cytokines. PCM decreases ATP production and increases ROS production in T cells by inhibiting complex III of the electron transport chain. We further show that SHP1 as the key molecule that is upregulated in T cells in response to PCM, inhibition of which reverses the phenotype induced by PCM. Using metabolomics analysis, we identified 1-pyrroline-5-carboxylate (P5C) as a vital molecule that is released by PCC. P5C is responsible for suppressing T cells signaling by increasing ROS and SHP1, and decreasing cytokines and ATP production. We confirmed these findings in vivo, which revealed changed proline dehydrogenase (PRODH) expression in tumor tissues, which in turn influences tumor growth and T cell infiltration.

Conclusions: Our study uncovered a key immunosuppressive axis, which is triggered by PRODH upregulation in PCa tissues, P5C secretion in media and subsequent SHP1-mediated impairment of T cell signaling and infiltration in PCa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40425-018-0466-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291986PMC
December 2018

A comprehensive classification method for VOC emission sources to tackle air pollution based on VOC species reactivity and emission amounts.

J Environ Sci (China) 2018 May 9;67:78-88. Epub 2017 Aug 9.

Municipal Research Institute of Environmental Protection, Beijing 100037, China; Key Laboratory of Beijing on VOC Pollution Control Technology and Application of Urban Atmosphere, Beijing 100037, China.

In China, volatile organic compound (VOC) control directives have been continuously released and implemented for important sources and regions to tackle air pollution. The corresponding control requirements were based on VOC emission amounts (EA), but never considered the significant differentiation of VOC species in terms of atmospheric chemical reactivity. This will adversely influence the effect of VOC reduction on air quality improvement. Therefore, this study attempted to develop a comprehensive classification method for typical VOC sources in the Beijing-Tianjin-Hebei region (BTH), by combining the VOC emission amounts with the chemical reactivities of VOC species. Firstly, we obtained the VOC chemical profiles by measuring 5 key sources in the BTH region and referencing another 10 key sources, and estimated the ozone formation potential (OFP) per ton VOC emission for these sources by using the maximum incremental reactivity (MIR) index as the characteristic of source reactivity (SR). Then, we applied the data normalization method to respectively convert EA and SR to normalized EA (NEA) and normalized SR (NSR) for various sources in the BTH region. Finally, the control index (CI) was calculated, and these sources were further classified into four grades based on the normalized CI (NCI). The study results showed that in the BTH region, furniture coating, automobile coating, and road vehicles are characterized by high NCI and need to be given more attention; however, the petro-chemical industry, which was designated as an important control source by air quality managers, has a lower NCI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jes.2017.08.003DOI Listing
May 2018

CO suppresses prostate cancer cell growth by directly targeting LKB1/AMPK/mTOR pathway in vitro and in vivo.

Urol Oncol 2018 Jun 19;36(6):312.e1-312.e8. Epub 2018 Mar 19.

Department of Urology, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan China. Electronic address:

Background: CO is a freely diffusible gas that acts as a physiological mediator of many biological and cellular processes, which has been shown to possess anticancer effect in many kinds of cancers. However, the effect of CO on prostate cancer has not been demonstrated. Therefore, we analyzed the antitumor activities and related mechanisms of CO on prostate cancer in vitro and in vivo.

Methods: Cell viability of LNCaP and PC-3 cells after CORM-2 treatment was measured by CCK-8 assay, whereas the ATP production were detected by ATP detection assay. The early apoptosis induced by CO was evaluated by flow cytometry, and the expression level of apoptosis-related molecules (Caspases 3, 8, 9 and cleaved-Caspases 3, 8, 9) was detected using Western blot. Matrigel in vitro invasion assay was used to evaluate the effect of CO on cell invasion. We then evaluated the impact of CO on the expression of several key regulators involved in the LKB1 signaling pathway. At last, xenograft tumor in nude mice was used to further investigate the antitumor effect of CO in vivo.

Results: Our results showed that CO could significantly inhibit proliferation and invasion, and induce apoptosis in human prostate cancer cell lines. The expression of LKB1 could be up-regulated after CO treatment, and CO also could increase p-AMPK levels and decrease p-mTOR. Furthermore, LKB1 knockdown could weaken the effect of CO on prostate cancer cells. In vivo, CO treatment significantly suppressed tumor growth and induced apoptosis in xenografts tumor in nude mice.

Conclusions: CO possesses striking anticancer effect in human prostate cancer cells in vitro and in vivo, which is largely mediated by LKB1-AMPK-mTOR axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.urolonc.2018.02.013DOI Listing
June 2018

Characteristics of ozone and ozone precursors (VOCs and NOx) around a petroleum refinery in Beijing, China.

J Environ Sci (China) 2014 Feb;26(2):332-42

A field measurement campaign for ozone and ozone precursors (VOCs and NOx) was conducted in summer 2011 around a petroleum refinery in the Beijing rural region. Three observation sites were arranged, one at southwest of the refinery as the background, and two at northeast of the refinery as the downwind receptors. Monitoring data revealed the presence of serious surface O3 pollution with the characteristics of high average daily mean and maximum concentrations (64.0 and 145.4 ppbV in no-rain days, respectively) and multi-peak diurnal variation. For NOx, the average hourly concentrations of NO2 and NO were in the range of 20.5-46.1 and 1.8-6.4 ppbV, respectively. For VOC measurement, a total of 51 compounds were detected. Normally, TVOCs at the background site was only dozens of ppbC, while TVOCs at the downwind sites reached several hundreds of ppbC. By subtracting the VOC concentrations at background, chemical profiles of VOC emission from the refinery were obtained, mainly including alkanes (60.0% +/- 4.3%), alkenes (21.1% +/- 5.5%) and aromatics (18.9% +/- 3.9%). Moreover, some differences in chemical profiles for the same measurement hours were observed between the downwind sites; the volume ratios of alkanes with low reactivity and those of alkenes with high reactivity respectively showed an increasing trend and a decreasing trend. Finally, based on temporal and spatial variations of VOC mixing ratios, their photochemical degradations and dispersion degradations were estimated to be 0.15-0.27 and 0.42-0.62, respectively, by the photochemical age calculation method, indicating stronger photochemical reactions around the refinery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/s1001-0742(13)60412-xDOI Listing
February 2014

Characterization of ambient ozone and its precursors around a coking plant.

Environ Monit Assess 2014 May 7;186(5):3165-79. Epub 2014 Feb 7.

Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing, 100124, China.

The local-scale relationship between ambient ozone (O3) and its precursors was examined around a coking plant in northern China. The upwind, plant boundary, and downwind locations were selected for investigation during the summer and autumn seasons in 2012. It was found that propene, toluene, and benzene were the top three non-methane hydrocarbon (NMHC) species for O3 formation at plant boundary, while propene, toluene, and m/p-xylene were the top three NMHC species at downwind location. Isoprene was the dominant species for O3 formation at upwind location. It was also found that an O3 depressing process occurred at plant boundary as a result of high NO emissions. Both local photochemistry and transport led to O3 accumulation at the downwind locations. The variation of NMHC concentration during O3 polluted and non-polluted episodes was investigated, and it indicated that NMHC concentration was higher during non-polluted episodes than polluted episodes. The impacts of precursors on O3 formation under different meteorological conditions were also examined.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10661-013-3608-2DOI Listing
May 2014

Restoration of LRIG1 suppresses bladder cancer cell growth by directly targeting EGFR activity.

J Exp Clin Cancer Res 2013 Dec 8;32:101. Epub 2013 Dec 8.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Background: Recently, leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1), a negative regulator of EGFR, was discovered is a novel agent for suppressing bladder cancer. The aim of this study was to investigate the impact of LRIG1 on the biological features of aggressive bladder cancer cells and the possible mechanisms of enhanced apoptosis induced by upregulation of LRIG1.

Methods: In this study, we examined the mRNA and protein expression of LRIG1 and EGFR in bladder cancers and normal bladder. Meanwhile, we overexpressed LRIG1 with adenovirus vector in T24/5637 bladder cancer cell lines, and we used real time-PCR, western blot, and co-immunoprecipitation analysis in order to examine the effects of LRIG1 gene on EGFR. Furthermore, we evaluate the impact of LRIG1 gene on the function of human bladder cancer cells and EGFR signaling.

Results: The expression of LRIG1 was decreased, while the expression of EGFR was increased in the majority of bladder cancer, and the ratio of EGFR/LRIG1 was increased in tumors versus normal tissue. We found that upregulation of LRIG1 induced cell apoptosis and cell growth inhibition, and further reversed invasion in bladder cancer cell lines in vitro by inhibiting phosphorylation of downstream MAPK and AKT signaling pathway.

Conclusion: Taken together, our findings provide us with an insight into LRIG1 function, and we conclude that LRIG1 evolved in bladder cancer as a rare feedback negative attenuator of EGFR, thus could offer a novel therapeutic target to treat patients with bladder cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/1756-9966-32-101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3880093PMC
December 2013

Poly(ADP-Ribose) polymerase inhibition improves erectile function in diabetic rats.

J Sex Med 2011 Apr 30;8(4):1002-14. Epub 2010 Aug 30.

Department of Urology, the Central Hospital of Wuhan, Wuhan, Hubei, China.

Introduction: Erectile dysfunction (ED) is a common and hard-to-treat complication of diabetes mellitus (DM). Multiple lines of evidence have shown that poly(ADP-ribose) polymerase (PARP) activation plays an important role in neurovascular dysfunction in diabetes, which is the crucial mechanism for diabetic ED.

Aim: To investigate the preventive benefit of a PARP inhibitor in a rat model of ED induced by diabetes.

Methods:   Established streptozotocin-diabetic male Sprague-Dawley rats were given PJ-34, a selective PARP inhibitor, by oral gavage at a dose of 10 mg/kg twice daily for 8 weeks. Erectile responses under electrical stimulation of the cavernous nerve, PARP activity and reactive oxygen species (ROS) production were measured. Nitric oxide synthase (NOS) isoforms were evaluated by Western blot and real-time quantitative PCR. Nuclear factor-kappa B activition and apoptosis in corpus cavernosa (CC) were also investigated.

Main Outcome Measures: The effects of PARP inhibition on the development of diabetic ED were determined.

Results: Diabetes markedly attenuated the erectile responses (intracavernosal pressure/mean systemic arterial blood pressure) and these were partially prevented by PJ-34 treatment. Promoted oxidative stress associated PARP activation was found in CC from vehicle-treated diabetic rats. PJ-34 blocked PARP activity and the diabetes-associated ROS generation. Decreased expression and activity of constitutive NOS (cNOS), including endothelial NOS (eNOS) and neuronal NOS (nNOS), associated with enhanced inducible NOS (iNOS) expression and activity were observed in vehicle-treated diabetic rats. Although PJ-34 had no effect on eNOS expression, it significantly prevented the decrease in nNOS expression and cNOS activity, and inhibited iNOS expression and activity in diabetic rats. PARP blockade by PJ-34 to some extent prevented diabetes-associated apoptosis and NF-κB activation.

Conclusions: Our results indicate that PARP activation plays an important role in the pathogenesis of diabetic ED and PARP inhibition may be a promising strategy to prevent development of diabetic ED.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1743-6109.2010.01963.xDOI Listing
April 2011