Publications by authors named "Guofang Wang"

13 Publications

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Mesoporous silica-mediated controllable electrochemiluminescence quenching for immunosensor with simplicity, sensitivity and tunable detection range.

Talanta 2021 Aug 9;231:122399. Epub 2021 Apr 9.

Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha, 410013, Hunan, China. Electronic address:

Straightforward and accurate measurement of medical biomarkers is of essential importance in clinical diagnostics and treatments. However, the major challenge is the diversity in dynamic range of different biomarkers ranging from pg mL to μg mL in various body fluids and tissues among patients. Here, we develop a mesoporous silica (MS)-mediated controllable electrochemiluminescence (ECL) quenching of immunosensor that allows accurate immunoassays with simplicity, sensitivity and tunable sensing range. MS is employed to enhance the sensitivity and tune ECL quenching to broaden the detection range just by altering luminophore (Ru(bpy)) and coreactant (DBAE) concentration without additional modifications. The immunoassay is followed: homogeneous sandwich immunoreaction, magnetic separation, and ECL quenching detection. As a proof-of-concept, simple and sensitive detection of IgG is achieved ranging from pg mL to μg mL, and applications of the strategy are extended by the combination of ECL immunosensor with commercial ELISA kit. This study will not only be expected to serve as a new avenue for the assay of physiological and clinical implications of immunological biomarkers, but also benefit a wide range of applications that require a tunable detection range and ultrahigh sensitivity.
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http://dx.doi.org/10.1016/j.talanta.2021.122399DOI Listing
August 2021

Microplastic Pollution in China, an Invisible Threat Exacerbated by Food Delivery Services.

Bull Environ Contam Toxicol 2020 Oct 19. Epub 2020 Oct 19.

College of Environment, Zhejiang University of Technology, Hangzhou, 310014, China.

With the rapid development and democratization of the internet and smart phone industry, online food delivery services have become increasingly popular all over the globe, namely in China. One of the unfortunate drawbacks of these delivery services is that they mainly use single-use plastics as food packaging, therefore generating large amounts of disposable food containers to meet demand. Such plastic containers reach the end of their service life after a single meal, and are then discarded as plastic waste. The sheer amount of plastic food containers discarded in this manner exacerbates various environmental issues, including one that is invisible to the naked eye: microplastic pollution. This minireview summarizes the history of food delivery services in China, from orders made face-to-face to digital orders, as well as the consequences introduced by the tremendous amounts of plastic waste generated by the food delivery services. Microplastic pollution could be mitigated to a certain extent by improving the classification, handling and management of single-use plastic containers in China. Furthermore, additional studies focusing on microplastic pollution caused by food delivery services are needed, especially as the use of these services is on the rise worldwide.
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http://dx.doi.org/10.1007/s00128-020-03018-1DOI Listing
October 2020

Trichoepithelioma: Reflectance confocal microscopy features and dermoscopic features.

Skin Res Technol 2021 Mar 23;27(2):283-284. Epub 2020 Jul 23.

Department of Dermatology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhenzhou University, Zhengzhou, China.

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http://dx.doi.org/10.1111/srt.12935DOI Listing
March 2021

PDK-1 mediated Hippo-YAP-IRS2 signaling pathway and involved in the apoptosis of non-small cell lung cancer cells.

Biosci Rep 2019 05 14;39(5). Epub 2019 May 14.

Respiratory Medicine, Yan'an University Affiliated Hospital, Yan'an 716000, China

Pyruvate dehydrogenase kinase-1 (PDK-1), a gatekeeper enzyme, was involved in cancer progression, such as tumor angiogenesis, cell survival, and growth. Recent evidence indicated that PDK-1 may be involved in lung cancer, however, the function and underlying mechanism of PDK-1 is remaining unclear. In the present study, our aim was to investigate the role and mechanisms of PDK-1 in human non-small cell lung cancer (NSCLC) cells. We first observed that PDK-1 was highly expressed in NSCLC cell lines. PDK-1 silence resulted in the inhibition of NSCLC cell survival. Also, cell apoptosis and caspase-3 activity were increased by PDK-1 knockdown in H1299 and A549 cells. Attenuation of PDK-1 expression blocked YAP and insulin receptor substrate 2 (IRS2) expression, and PDK-1 silence suppressed IRS2 expression dependent on Hippo-YAP signaling pathway. Moreover, further studies confirmed that YAP or IRS2 overexpression reversed the action of PDK-1 in NSCLC cells. In conclusion, our findings indicate that PDK1/Hippo-YAP/IRS2 signaling pathway plays a critical role in NSCLC cell survival and apoptosis.
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http://dx.doi.org/10.1042/BSR20182099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522739PMC
May 2019

miR-143 suppresses the osteogenic differentiation of dental pulp stem cells by inactivation of NF-κB signaling pathway via targeting TNF-α.

Arch Oral Biol 2018 Mar 30;87:172-179. Epub 2017 Dec 30.

Department of Periodontology, The First Affiliated Hospital & School of Stomatology, Zhengzhou University, Zhengzhou, 450052, China.

Background: Dental pulp stem cells (DPSCs) are multipotent and play an important role in repairing damaged and/or defective dentinogenesis/osteogenesis. Recent studies have documented the implication of miR-143 in osteogenic differentiation of DPSCs. Nevertheless, the detailed mechanisms of miR-143 involved in the osteogenic differentiation of DPSCs remain to be further elaborated.

Methods: Isolated DPSCs were incubated with osteogenic differentiation medium to induce osteogenic differentiation. qRT-PCR and western blot were performed to determine the expressions of miR-143 and tumor necrosis factor α (TNF-α). Luciferase reporter assay was used to confirm whether TNF-α was a target of miR-143. Osteogenic differentiation of DPSCs was evaluated by alkaline phosphatase (ALP) activity assay, ALP staining, and western blot analyses of osteogenic-markers including bone morphogenetic protein 2 (BMP2), ALP, runt-related transcription factor 2 (RUNX2) and collagen type I (COLI).

Results: miR-143 was downregulated and TNF-α was upregulated during osteogenic differentiation of DPSCs. miR-143 posttranscriptionally regulated TNF-α expression in DPSCs by binding to its 3'UTR. miR-143 overexpression suppressed osteogenic differentiation of DPSCs, as demonstrated by the decrease of ALP activity, ALP positive cell ratio, as well as BMP2, ALP, RUNX2, and COLI expressions. Moreover, miR-143 reversed TNF-α-induced osteogenic differentiation of DPSCs. Finally, the osteogenic differentiation of DPSCs induced by miR-143 inhibitor was attenuated following inactivation of nuclear factor kappa B (NF-κB) signaling pathway.

Conclusion: miR-143 suppressed the osteogenic differentiation of DPSCs by blockade of NF-κB signaling pathway via targeting TNF-α.
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http://dx.doi.org/10.1016/j.archoralbio.2017.12.031DOI Listing
March 2018

Measuring generalised expectancies for negative mood regulation in China: The Chinese language Negative Mood Regulation scale.

Int J Psychol 2019 Apr 6;54(2):223-231. Epub 2017 Aug 6.

Department of Psychology, Illinois State University, Normal, IL, USA.

Negative mood regulation expectancies (NMRE) represent people's confidence that they can alleviate their negative affect or induce a positive emotional state through thought or action. NMRE predict coping behaviour and mood outcomes for individuals under stress. Since 1990, much research documents the reliability and validity of the English language Negative Mood Regulation (NMR) scale as a measure of NMRE. The current research reports two studies developing a Chinese language translation of the NMR (NMR-C) scale that goes beyond literal translation to be a culturally sensitive measure of NMRE in China. In Study 1, 713 college students from both a major city and a rural setting in China were surveyed. Data support the resulting 32-item NMR-C's reliability (alpha = .88) and validity. The NMR-C showed both direct and indirect links to depression and anxiety; coping mediated the indirect effect. In Study 2, 331 prison police officers in three Chinese provinces participated. NMRE buffered the effect of high role pressure, moderating the relationship between prison police role stress and job engagement. Results of the two studies support the reliability and validity of the Chinese language NMR scale and parallel results found with measures of NMRE in the West and in other Asian countries.
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http://dx.doi.org/10.1002/ijop.12443DOI Listing
April 2019

Overview of Cantharidin and its Analogues.

Curr Med Chem 2018 ;25(17):2034-2044

Department of Chemistry and Chemical Engineering, Shaoxing University, Shaoxing 312000, China.

Background: Cantharidin has been categorized as highly toxicant in Chinese medicine. But cantharidin can efficiently treat different types of diseases, such as molluscum contagiosum. While cantharidin is quite useful, unfortunately, due to its side effects, increasing regulations have limited access to this useful therapeutic option. Cantharidin's toxic effects have caused it to fall into disuse for most legitimate medical purposes. Although cantharidin generates effects and its advantages must be realized. Recently, cancer affects people's life more and more. Because cantharidin can treat some cancers, so solutions must be used to reduce side effects. This review aims to describe some its analogues, several efficient methods to inhibit the side effects of cantharidin and pharmacogenomics of cantharidin.

Methods: We searched for research about cantharidin by entering the database. Then evaluated these papers and analyzed their founding, solution, mechanism, etc., and targeted to screen the papers related to the content of our research, and then sorted them out in accordance with the solution, mechanism research and other content. Finally, these content was unified into a framework.

Results: Some cantharidin's analogues were found that they show some similar functions to cantharidin and we found that norcantharidin, acylthiourea derivatives, cantharidinamides, anhydride-modified derivatives and other derivatives have less side effects. The modified cantharidin analogues reduce toxicity in hepatocytes. Cantharidin consists of a six-ring and a five-ring, the moiety of oxygen on the six-ring and the anhydride section exhibit biochemical activity. Protein phosphatases are associated with many cellular processes including apoptosis, cell cycle progression and so on. Cantharidin can cause apoptosis and double-stand breakage of DNA. Cantharidin and norcantharidin can efficiently inhibit the activity of mammalian and plant protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) in vivo. Cantharidin inhibits PP5 at the nanomolar level with an IC50 value of 600 nM. PP5 can manage the cellular survival, death, proliferation and other some intracellular biological activities in mammals. After cantharidin's treatment, the level of EtPP5 mRNA expression was downregulated. Their also can be used to inhibit the Glutathione S-transferases (GSTs), angiogenesis and the expression of A549 human lung cancer cells, trigger eryptosis and induced bladder cancer cell apoptosis. We found that using Vitamin C and ginsenosides and translating cantharidin into nanoparticles can minimize the cantharidin side effects in the patients.

Conclusion: Cantharidin can inhibit various tumor cell lines. Cantharidin causes both DNA single- and double- strand breaks and induces apoptosis. Although cantharidin shows some toxicity for human, its anti-cancer effects should be taken seriously. Several viable methods can help solve this problem. The most important pharmacogenomics of cantharidin is that cantharidin can inhibit PPs, because PPs are associated with many cellular processes. This prospect is very broad and needs to continue studying.
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http://dx.doi.org/10.2174/0929867324666170414165253DOI Listing
November 2018

A Novel De novo Mutation of the SASH1 Gene in a Chinese Family with Multiple Lentigines.

Acta Derm Venereol 2017 04;97(4):530-531

Department of Dermatology, Henan Provincial People's Hospital, No.7 Weiwu Road, Zhengzhou 450003, China.

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http://dx.doi.org/10.2340/00015555-2575DOI Listing
April 2017

Dual emission of Ce ,Mn -coactivated Ca YNa(PO ) F via energy transfer: a single component white/yellow-emitting phosphor.

Luminescence 2017 Jun 15;32(4):529-534. Epub 2016 Sep 15.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning, People's Republic of China.

A series of Ce ,Mn -coactivated Ca YNa(PO ) F phosphors were synthesized via a traditional solid-state reaction under a reductive atmosphere. X-Ray powder diffraction was used to confirm that the crystal structure and diffraction peaks of Ce /Mn -doped samples matched well with the standard data. A spectral overlap between the emission band of Ce and the excitation band of Mn suggested the occurrence of energy transfer from Ce to Mn . With increasing Mn content, the emission intensities and lifetime values of the Ce emission for Ca YNa(PO ) F:Ce ,Mn phosphors linearly decrease, whereas the energy transfer efficiencies gradually increase to 89.35%. By adjusting the relative concentrations of Ce and Mn , the emission hues are tuned from blue to white and eventually to yellow. These results suggest that Ca YNa(PO ) F:Ce ,Mn phosphors have promising application as white-emitting phosphors for near-ultraviolet light-emitting diodes.
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http://dx.doi.org/10.1002/bio.3208DOI Listing
June 2017

Color-tunable emission and energy transfer investigation in SrY(PO):Ce,Tb phosphors for white LEDs.

Spectrochim Acta A Mol Biomol Spectrosc 2017 Feb 2;173:53-58. Epub 2016 Aug 2.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.

A novel color-tunable phosphor SrY(PO):Ce,Tb was synthesized through solid-state reaction method. Several techniques, such as X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy, were used to investigate the obtained phosphors. Results of luminescence spectra and decay time measurements revealed that an efficient energy transfer occurred from Ce to Tb via a dipole-dipole mechanism, where Ce exhibited a strong excitation band in the near-ultraviolet region. CIE chromaticity coordinates were tuned from deep blue (0.162, 0.090) to green (0.230, 0.411) by adjusting the relative concentrations between Ce and Tb ions. Results revealed that the as-synthesized phosphors had color-tunable characteristics and can be used as promising materials in the field of phosphor-converted white light-emitting diodes.
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http://dx.doi.org/10.1016/j.saa.2016.07.047DOI Listing
February 2017

Luminescence and energy transfer of the color-tunable phosphor Li₆Gd(BO₃)₃:Tb³⁺/Bi³⁺, Eu³⁺.

Appl Radiat Isot 2016 Feb 17;108:148-153. Epub 2015 Dec 17.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. Electronic address:

Bi(3+)/Tb(3+), Eu(3+) co-doped Li6Gd(BO3)3 (LGBO) phosphors were synthesized via a traditional solid-state method. Phase purity was investigated using X-ray diffraction, absorption strength of the phosphors were investigated by UV-vis absorption spectra, and the photoluminescence properties of the phosphors were studied systematically. Results showed that the emission intensity of Bi(3+), Eu(3+) co-doped LBOG was 2.76 times higher than that of Eu(3+)-doped LGBO irradiated at 275 nm, thereby implying the possibility of energy transfer from Bi(3+) to Eu(3+). The excitation spectra of Tb(3+), Eu(3+) co-doped LGBO phosphors are broader in comparison with single-doped phosphors and show tunable colors from green to yellow to orange-red when the ratio of Tb(3+) to Eu(3+) is adjusted These results demonstrate that LGBO:Tb(3+), Eu(3+) phosphors have potential use in light-emitting diodes.
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http://dx.doi.org/10.1016/j.apradiso.2015.12.042DOI Listing
February 2016

Luminescence and energy transfer of color-tunable Li6Gd(BO3)3:Ce(3+), Tb(3+) phosphor.

Spectrochim Acta A Mol Biomol Spectrosc 2015 Oct 11;149:682-6. Epub 2015 May 11.

School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China. Electronic address:

A series of novel color-tunable phosphors of Ce(3+), Tb(3+)-codoped Li6Gd(BO3)3 was synthesized through a classic solid-state reaction. The color of these phosphors changes from blue to green by adjusting the ratio of Ce(3+) to Tb(3+). The photoluminescence properties of the synthesized phosphors were investigated, and several major emission bands that belong to Ce(3+) and Tb(3+) ions were irradiated with near ultraviolet light. Moreover, the energy transfer mechanism between Ce(3+) and Tb(3+) in Li6Gd(BO3)3 was explored. The photoluminescence decay curves were performed to validate the energy transfer. The analysis demonstrated that the energy transfer from Ce(3+) to Tb(3+) arose from dipole-dipole interaction with a critical distance of approximately 17.6 Å.
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http://dx.doi.org/10.1016/j.saa.2015.04.108DOI Listing
October 2015

Analysis on the formation condition of the algae-induced odorous black water agglomerate.

Saudi J Biol Sci 2014 Dec 15;21(6):597-604. Epub 2014 Jul 15.

School of Energy and Environment, Southeast University, Sipailou 2#, Nanjing 210096, PR China.

The algae-induced odorous black water agglomerate (OBWA) is a phenomenon in which water turns black and emits odorous gas. It is an ecological and environmental problem that has occurred several times in Taihu, a large eutrophic shallow lake in China. In this study, the collected eutrophic water with different algae densities was used to simulate OBWA. The results revealed that the massive accumulation and death of algae was the substrate source for OBWA. When the algae density reached 1.0 × 10(8) cells/L in the static and dark condition, at a constant high temperature (30 ± 2 °C), OBWA happened. There was a time difference between the water stinking and blackening with the stinking first. When the oxidation-reduction potential (ORP) value was between -250 and -50 mV, Dimethyl trisulfide (DMTS), the main contributor to the water stinking at the initial stage, and other odorous organics were produced. Water blackening was closely related to the increases of sulfide and dissolved Fe(2+) concentration. When the ORP value was between -350 and -300 mV, heavy metal containing sulfides such as FeS formed. Therefore, the condition when the water ORP value decreased to about -300 mV was considered the precursor for OBWA formation.
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http://dx.doi.org/10.1016/j.sjbs.2014.07.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250508PMC
December 2014