Publications by authors named "Guobin Zhang"

95 Publications

Effects of different vegetable rotations on the rhizosphere bacterial community and tomato growth in a continuous tomato cropping substrate.

PLoS One 2021 23;16(9):e0257432. Epub 2021 Sep 23.

College of Horticulture, Gansu Agricultural University, Lanzhou, China.

In this study, High throughput sequencing was used to analyze the effects of different vegetable rotations on the rhizosphere bacterial diversity and community structure in a substrate that was used for continuous tomato cropping (CK). The vegetable rotations tested were cabbage/tomato (B), kidney bean/tomato (D), and celery/tomato (Q). The results revealed that the substrate bacterial diversity and richness of each crop rotation were higher than those of CK. The highest bacterial diversity was found in the B substrate, followed by the Q and D substrates. Further comparison showed that the rhizosphere bacterial community structure of Q substrate was significantly different to that of CK. Compared with the CK, the Q substrate had a significantly higher relative abundance of several dominant microflora, such as Acidobacteria, Chloroflexi, and Firmicutes. Additionally, the Q rotation significantly increased the abundance of beneficial bacteria, such as Actinobacteria_unclassified and Anaerolineaceae_unclassified. A redundancy analysis showed that Most dominant bacteria correlated positively with the substrate pH, total N, and alkali-hydrolyzable N but negatively with the available P, available K, total P, total K, and organic matter contents and substrate EC. The substrates after crop rotation improved the growth and physiological condition of the subsequent tomato plants, among which those from the Q rotation performed the best. Therefore, celery rotation not only increased the richness and diversity of bacterial communities in the substrate but also significantly increased the richness of the beneficial bacterial communities, allowing better maintenance of the substrate microenvironment for the healthy growth of crops.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257432PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459948PMC
September 2021

Association of postoperative delirium with cognitive outcomes: A meta-analysis.

J Clin Anesth 2021 Sep 2;75:110496. Epub 2021 Sep 2.

Department of Critical Care Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. Electronic address:

Study Objective: To determine the association between postoperative delirium (POD) and cognitive outcomes at least 1 month after surgery in elderly patients, and synthesize the dynamic risk trajectory of cognition impairment after POD.

Design: Meta-analysis searching PubMed, Cochrane and EMBASE from inception to November 1, 2020. The terms postoperative delirium, delirium after surgery, postsurgical delirium, postoperative cogniti*, postoperative cognitive dysfunction, postoperative cognition decline, cognitive decline, cognitive impair* and dement* were searched alone or in combination.

Measurements: Inclusion criteria were prospective cohort studies investigating the association between POD and cognitive outcomes in patients aged ≥60 years underwent surgery. The primary outcome was the association between POD and cognitive outcomes at 1 or more months after surgery. We considered cognitive outcomes measured up to 12 months after surgery as short-term and beyond 12 months as long-term. Two authors performed the study screening, data extraction and quality assessments. Effect sizes were calculated as Hedges g or Odds ratio (OR) based on random- and fixed-effects models. Meta-regression was conducted to analyze the role of potential contributors to heterogeneity.

Main Results: Eighteen studies were included. Our result showed a significant and medium association between POD and cognitive outcomes after at least 1 month postoperatively (g = 0.61 95% CI 0.43-0.79; I = 65.1%), indicating that patients with POD were associated with worse cognitive outcomes. The association of POD with short- and long-term cognitive impairment were also both significant (short-term: g = 0.46 95% CI 0.24-0.68; I = 53.1%; and long-term: g = 0.82 95% CI 0.57-1.06; I = 57.1%). A multivariate meta-regression suggested that age and measure of delirium were significant sources of heterogeneity. POD was also associated with the significant risk for dementia (OR = 6.08 95% CI 3.80-9.72; I = 0) as well as attention (OR = 1.74 95% CI 1.13-2.68; I = 0), executive (OR = 1.33 95% CI 1.00-1.80; I = 0) and memory impairment (OR = 1.59 95% CI 1.20-2.10; I = 43.0%). Additionally, our results showed that the risk trajectory for cognitive decline associated with POD within five years after surgery revealed exponential growth.

Conclusions: This is the first meta-analysis quantifying the association between POD and cognitive outcomes. Our results showed that POD was significantly associated with worse cognitive outcomes, including short- and long-term cognitive outcomes following surgery.
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http://dx.doi.org/10.1016/j.jclinane.2021.110496DOI Listing
September 2021

Photoassisted Electron-Ion Synergic Doping Induced Phase Transition of n-VO/p-GaN Thin-Film Heterojunction.

ACS Appl Mater Interfaces 2021 Sep 1;13(36):43562-43572. Epub 2021 Sep 1.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, P. R. China.

As a typical correlated metal oxide, vanadium dioxide (VO) shows specific metal-insulator transition (MIT) properties and demonstrates great potential applications in ultrafast optoelectronic switch, resistive memory, and neuromorphic devices. Effective control of the MIT process is essential for improving the device performance. In the current study, we have first proposed a photoassisted ion-doping method to modulate the phase transition of the VO layer based on the photovoltaic effect and electron-ion synergic doping in acid solution. Experimental results show that, for the prepared n-VO/p-GaN nanojunction, this photoassisted strategy can effectively dope the n-VO layer by H, Al, or Mg ions under light radiation and trigger consecutive insulator-metal-insulator transitions. If combined with standard lithography or electron beam etching processes, selective doping with nanoscale size area can also be achieved. This photoassisted doping method not only shows a facile route for MIT modulation via a doping route under ambient conditions but also supplies some clues for photosensitive detection in the future.
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http://dx.doi.org/10.1021/acsami.1c10401DOI Listing
September 2021

An inverse planning simulated annealing algorithm with adaptive weight adjustment for LDR pancreatic brachytherapy.

Int J Comput Assist Radiol Surg 2021 Aug 28. Epub 2021 Aug 28.

School of Mechanical Engineering, Tianjin University, 135 Yaguan Road, Jinnan District, Tianjin, 300350, China.

Purpose: The inverse planning simulated annealing (IPSA) algorithm has shown good results in cancer surgical treatment planning. However, an adaptive approach has not well been proposed for different shapes and sizes of tumors. The purpose of this study was to propose an adaptive, efficient and safe algorithm to get high-quality treatment dose planning, which is presented for pancreatic cancer.

Methods: An algorithm employs an optimized IPSA and an adaptive process for adjusting the weight of organs at risk (OAR) and tumor. The algorithm, which was combined with ant colony optimization, was further optimized to reduce the number of needles. It could meet the clinical dose objectives within the tumors, reduce the dose distribution within the OAR and minimize the number of needles. Ten clinical cases were chosen randomly from patients, previously successfully treated in clinic to test our method. The algorithm was validated against clinical cases, using clinically relevant dose parameters.

Results: The results were compared with clinical results in ten cases, indicating that the dose distribution within the tumor meets the clinical dose objectives. The dose received by OAR had been greatly reduced, and the number of needles could be reduced by about 50%. It was a significant improvement over the clinical treatment planning.

Conclusions: In this paper, we have devised an algorithm to optimize the treatment planning in brachytherapy. The method in this paper could meet the clinical dose objectives and reduce the difficulty of operation. The results were clinically acceptable. This algorithm is also applicable to other cancers such as lung cancer.
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http://dx.doi.org/10.1007/s11548-021-02483-1DOI Listing
August 2021

Multiorgan segmentation from partially labeled datasets with conditional nnU-Net.

Comput Biol Med 2021 Sep 21;136:104658. Epub 2021 Jul 21.

School of Mechanical Engineering, Tianjin University, Tianjin, 300350, China. Electronic address:

Accurate and robust multiorgan abdominal CT segmentation plays a significant role in numerous clinical applications, such as therapy treatment planning and treatment delivery. Almost all existing segmentation networks rely on fully annotated data with strong supervision. However, annotating fully annotated multiorgan data in CT images is both laborious and time-consuming. In comparison, massive partially labeled datasets are usually easily accessible. In this paper, we propose conditional nnU-Net trained on the union of partially labeled datasets for multiorgan segmentation. The deep model employs the state-of-the-art nnU-Net as the backbone and introduces a conditioning strategy by feeding auxiliary information into the decoder architecture as an additional input layer. This model leverages the prior conditional information to identify the organ class at the pixel-wise level and encourages organs' spatial information recovery. Furthermore, we adopt a deep supervision mechanism to refine the outputs at different scales and apply the combination of Dice loss and Focal loss to optimize the training model. Our proposed method is evaluated on seven publicly available datasets of the liver, pancreas, spleen and kidney, in which promising segmentation performance has been achieved. The proposed conditional nnU-Net breaks down the barriers between nonoverlapping labeled datasets and further alleviates the problem of data hunger in multiorgan segmentation.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104658DOI Listing
September 2021

The Roles of Cruciferae Glucosinolates in Disease and Pest Resistance.

Plants (Basel) 2021 May 30;10(6). Epub 2021 May 30.

Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.

With the expansion of the area under Cruciferae vegetable cultivation, and an increase in the incidence of natural threats such as pests and diseases globally, Cruciferae vegetable losses caused by pathogens, insects, and pests are on the rise. As one of the key metabolites produced by Cruciferae vegetables, glucosinolate (GLS) is not only an indicator of their quality but also controls infestation by numerous fungi, bacteria, aphids, and worms. Today, the safe and pollution-free production of vegetables is advocated globally, and environmentally friendly pest and disease control strategies, such as biological control, to minimize the adverse impacts of pathogen and insect pest stress on Cruciferae vegetables, have attracted the attention of researchers. This review explores the mechanisms via which GLS acts as a defensive substance, participates in responses to biotic stress, and enhances plant tolerance to the various stress factors. According to the current research status, future research directions are also proposed.
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http://dx.doi.org/10.3390/plants10061097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229868PMC
May 2021

Differential Predictors and Clinical Implications Associated With Long-Term Survivors in IDH Wildtype and Mutant Glioblastoma.

Front Oncol 2021 13;11:632663. Epub 2021 May 13.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Glioblastoma (GBM) is the most aggressive intracranial tumor which can be divided into two subtypes based on status of isocitrate dehydrogenase (IDH). A small fraction of patients after receiving standard treatment can be long-term survivors (LTS). This study was designed to disclose the predictors and clinical implications associated with LTS in IDH wildtype and mutant GBM.

Methods: Patients who survived beyond five years after diagnosis of GBM were defined as LTS, while those with a survival less than one year were defined as short-term survivors (STS). A total of 211 patients with diagnosis of GBM in Beijing Tiantan Hospital from January 2007 to January 2015 were enrolled, including 44 (20.9%) LTS and 167 (79.1%) STS. The clinical, radiological and molecular features between groups were systematically compared.

Results: Compared with STS, LTS were a subgroup of patients with a younger age at diagnosis (=0.006), a higher KPS score (=0.011), higher rates of cystic change (=0.037), O-methylguanine-DNA methyltransferase (MGMT) promoter methylation (=0.007), and IDH mutation (=0.049), and more likely to have undergone gross total resection (<0.001). Survival analysis demonstrated that LTS with wildtype IDH conferred a longer progression-free survival (66.0 27.0 months, =0.04), but a shorter post-progression survival (46.5 months not reached, =0.0001) than those of LTS with mutant IDH. LTS with mutant IDH showed a trend towards increased survival after receiving re-operation (=0.155) and reirradiation (=0.127), while this clinical benefit disappeared in the subset of LTS with wildtype IDH (>0.05).

Conclusion: The prognostic value and therapeutic implications associated with LTS in GBM population significantly differed on the basis of IDH status. Our findings provide a new approach for physicians to better understand the two subtypes of GBM, which may assist in making more tailored treatment decisions for patients.
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http://dx.doi.org/10.3389/fonc.2021.632663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155513PMC
May 2021

The reference genome of Miscanthus floridulus illuminates the evolution of Saccharinae.

Nat Plants 2021 05 6;7(5):608-618. Epub 2021 May 6.

State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China.

Miscanthus, a member of the Saccharinae subtribe that includes sorghum and sugarcane, has been widely studied as a feedstock for cellulosic biofuel production. Here, we report the sequencing and assembly of the Miscanthus floridulus genome by the integration of PacBio sequencing and Hi-C mapping, resulting in a chromosome-scale, high-quality reference genome of the genus Miscanthus. Comparisons among Saccharinae genomes suggest that Sorghum split first from the common ancestor of Saccharum and Miscanthus, which subsequently diverged from each other, with two successive whole-genome duplication events occurring independently in the Saccharum genus and one whole-genome duplication occurring in the Miscanthus genus. Fusion of two chromosomes occurred during rediploidization in M. floridulus and no significant subgenome dominance was observed. A survey of cellulose synthases (CesA) in M. floridulus revealed quite high expression of most CesA genes in growing stems, which is in agreement with the high cellulose content of this species. Resequencing and comparisons of 75 Miscanthus accessions suggest that M. lutarioriparius is genetically close to M. sacchariflorus and that M. floridulus is more distantly related to other species and is more genetically diverse. This study provides a valuable genomic resource for molecular breeding and improvement of Miscanthus and Saccharinae crops.
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http://dx.doi.org/10.1038/s41477-021-00908-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238680PMC
May 2021

Postoperative Long-Term Independence Among the Elderly With Meningiomas: Function Evolution, Determinant Identification, and Prediction Model Development.

Front Oncol 2021 5;11:639259. Epub 2021 Mar 5.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Maintenance of postoperative long-term independence has value for older adults who undergo surgical procedures. However, independence has barely caught attention for the elderly with meningiomas. Preventing postoperative long-term independence decline in this population necessitates the identification of the factors related to this outcome and minimizing their implications. Therefore, we assessed the independence evolution and identified potential determinants and population.

Materials And Methods: From 2010 to 2016, elderly meningioma patients (≥65 years old) undergoing operation at Beijing Tiantan Hospital were included in our study. The primary outcome was 3-year (., long-term) postoperative independence measured by Karnofsky performance scale (KPS) score. We used univariate and multivariate analyses to determine the risk factors for postoperative long-term independence, and nomogram was established.

Results: A total of 470 patients were included eligibly. The distribution in each KPS was significantly different before and 3 years after resection ( < 0.001). Especially in patients with preoperative KPS 80 and 70, only 17.5 and 17.3% of the patients kept the same KPS after 3 years, and the remaining patients experienced significant polarization. The most common remaining symptom cluster correlated with postoperative long-term independence included fatigue (R = -0.795), memory impairment (R = -0.512), motor dysfunction (R = -0.636) and communication deficits (R = -0.501). Independent risk factors for postoperative long-term non-independence included: advanced age (70-74 65-69 OR: 2.631; 95% CI: 1.545-4.481 and ≥75 65-69 OR: 3.833; 95% CI: 1.667-8.812), recurrent meningioma (OR: 7.791; 95% CI: 3.202-18.954), location in the skull base (OR: 2.683; 95% CI: 1.383-5.205), tumor maximal diameter >6 cm (OR: 3.089; 95% CI: 1.471-6.488), nerves involved (OR: 3.144; 95% CI: 1.585-6.235), high risk of WHO grade and biological behavior (OR: 2.294; 95% CI: 1.193-4.408), recurrence during follow-up (OR: 10.296; 95% CI: 3.253-32.585), lower preoperative KPS (OR: 0.964; 95% CI: 0.938-0.991) and decreased KPS on discharge (OR: 0.967; 95% CI: 0.951-0.984) ( < 0.05). The discrimination and calibration of the nomogram revealed good predictive ability (C-index: 0.810).

Conclusion: Elderly meningioma patients might present significant polarization trend in maintaining long-term independence after surgery. Our findings will be helpful for guiding surgical management for the elderly with meningioma and provide proposals for early functional rehabilitation.
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http://dx.doi.org/10.3389/fonc.2021.639259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982808PMC
March 2021

Adaptive Admixture of HLA Class I Allotypes Enhanced Genetically Determined Strength of Natural Killer Cells in East Asians.

Mol Biol Evol 2021 05;38(6):2582-2596

Division of Biomedical Informatics and Personalized Medicine, University of Colorado, Anschutz Medical Campus, Aurora, CO, USA.

Human natural killer (NK) cells are essential for controlling infection, cancer, and fetal development. NK cell functions are modulated by interactions between polymorphic inhibitory killer cell immunoglobulin-like receptors (KIR) and polymorphic HLA-A, -B, and -C ligands expressed on tissue cells. All HLA-C alleles encode a KIR ligand and contribute to reproduction and immunity. In contrast, only some HLA-A and -B alleles encode KIR ligands and they focus on immunity. By high-resolution analysis of KIR and HLA-A, -B, and -C genes, we show that the Chinese Southern Han (CHS) are significantly enriched for interactions between inhibitory KIR and HLA-A and -B. This enrichment has had substantial input through population admixture with neighboring populations, who contributed HLA class I haplotypes expressing the KIR ligands B*46:01 and B*58:01, which subsequently rose to high frequency by natural selection. Consequently, over 80% of Southern Han HLA haplotypes encode more than one KIR ligand. Complementing the high number of KIR ligands, the CHS KIR locus combines a high frequency of genes expressing potent inhibitory KIR, with a low frequency of those expressing activating KIR. The Southern Han centromeric KIR region encodes strong, conserved, inhibitory HLA-C-specific receptors, and the telomeric region provides a high number and diversity of inhibitory HLA-A and -B-specific receptors. In all these characteristics, the CHS represent other East Asians, whose NK cell repertoires are thus enhanced in quantity, diversity, and effector strength, likely augmenting resistance to endemic viral infections.
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http://dx.doi.org/10.1093/molbev/msab053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136484PMC
May 2021

Stable and ultrasensitive analysis of organic pollutants and heavy metals by dried droplet method with superhydrophobic-induced enrichment.

Anal Chim Acta 2021 Mar 25;1151:338253. Epub 2021 Jan 25.

Department of Physics, City University of Hong Kong, Hong Kong, China. Electronic address:

Herein, a dried droplet method (DDM) with superhydrophobic-induced enrichment is reported for stable and ultrasensitive analysis of organic pollutants and heavy metals. A superhydrophobic (SHB) substrate was prepared as an analytical detection platform for the DDM. This SHB substrate was synthesized by sequentially coating polydimethylsiloxane (PDMS) and titanium dioxide nanoparticles (TiO NPs) onto glass substrate surface. In the droplet drying process, the SHB substrate was demonstrated to suppress the coffee ring effect (CRE) and enriched analyte concentration. Combining with Raman spectroscopy for analysis of methylene blue (MB), and with laser-induced breakdown spectroscopy (LIBS) for analysis of chromium (Cr), the results indicated high stability and ultra-sensitivity for organic pollutants and heavy metals detection. Overall, the DDM with superhydrophobic-induced enrichment has big potential in applications requiring stable and ultrasensitive analysis.
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http://dx.doi.org/10.1016/j.aca.2021.338253DOI Listing
March 2021

Natural Variation of Lignocellulosic Components in Biomass in China.

Front Chem 2020 5;8:595143. Epub 2020 Nov 5.

State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China.

Lignocellulose content is an important factor affecting the conversion efficiency of biomass energy plants. In this study, 179 accessions in China were used to determine the content of lignocellulose components in stems via acid hydrolysis and high-performance liquid chromatography. Results showed that the average lignocellulose content of wild germplasm resources was 80.27 ± 6.51%, and the average content of cellulose, hemicellulose, lignin, extracts, and total ash was 38.38 ± 3.52, 24.23 ± 4.21, 17.66 ± 1.56, 14.50 ± 5.60, and 2.53 ± 0.59%, respectively. The average lignocellulose content of , and the hybrids was 77.94 ± 6.06, 75.16 ± 4.98, 75.68 ± 3.02, 83.71 ± 4.78, 81.50 ± 5.23, and 74.72 ± 7.13%, respectively. In all the tested materials, the highest cellulose content was 48.52%, and the lowest was 29.79%. Hemicellulose had the maximum content of 34.23% and a minimum content of 15.71%. The highest lignin content was 23.75%, and the lowest was 13.01%. The lignocellulosic components of different ploidy materials were compared. The content of lignocellulosic components of diploid was higher than that of tetraploid , and the content of lignocellulosic components of diploid was lower than that of tetraploid . Analysis of the relationship between the changes in lignocellulosic components and geographical locations of showed that the holocellulose and hemicellulose content was significantly positive correlated with the latitude of the original growth location. Results indicated that the lignocellulosic components of resources in China are rich in genetic diversity.
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http://dx.doi.org/10.3389/fchem.2020.595143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674668PMC
November 2020

[Identification of a novel KIR3DL3*064 allele by cDNA cloning and sequencing].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Aug;37(8):895-897

Shenzhen Blood Center, Shenzhen, Guangdong 518035, China.

Objective: To report on a novel KIR3DL3 allele identified in a southern Han Chinese individual.

Methods: Peripheral blood sample was collected from a voluntary blood donor with inconclusive result by KIR3DL3 sequence-based typing (SBT). Total mRNA was extracted and subjected to reverse transcription to obtain KIR3DL3 cDNA, which was then amplified by PCR with a pair of KIR3DL3-specific primers. The product was subjected to cDNA cloning and sequencing.

Results: cDNA cloning and sequencing have identified a wide-type KIR3DL3*00802 allele and a novel KIR3DL3*064 allele. The latter differed from KIR3DL3*00601 by a missense variant at codon 374[c.1184 C>T (p.Thr374Ile)] in exon 9. The novel KIR3DL3 allele has been officially assigned by the KIR subcommittee of World Health Organization Nomenclature Committee for factors of HLA system.

Conclusion: cDNA cloning and sequencing may be used to distinguish inconclusive results in KIR3DL3 SBT in order to identify novel KIR alleles.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2020.08.022DOI Listing
August 2020

Functional suppression of Ripk1 blocks the NF-κB signaling pathway and induces neuron autophagy after traumatic brain injury.

Mol Cell Biochem 2020 Sep 14;472(1-2):105-114. Epub 2020 Jul 14.

Department of Neurosurgery, Tianjin Huanhu Hospital, No. 6, Jizhao Road, Jinnan District, Tianjin, 300350, People's Republic of China.

Traumatic brain injury (TBI), known as intracranial injury, has been a serious threat to human health. Evidence exists indicating that autophagy and inflammatory responses contribute to secondary brain injury after TBI. Notably, receptor-interacting protein kinase 1 (Ripk1) exerts an important role in cell autophagy. Therefore, this study aims to explore the effect of Ripk1 on neuron autophagy and apoptosis in TBI. Initially, blood samples of patients with TBI and healthy persons were collected to detect the expression of Ripk1, nuclear factor-kappa B (NF-κB), and NF-kB inhibitor α (IKBα). Then rat models with TBI were successfully established and, respectively, treated with shRNA targeting Ripk1 (sh-Ripk1), Ripk1 overexpression plasmid (oe-Ripk1), or IKKα inhibitor (BAY 11-7082). Subsequently, reverse transcription quantitative polymerase chain reaction and Western blot analysis were conducted to detect the expression of Ripk1, IKBα, NF-κB signaling pathway-, and apoptosis-related factors. Enzyme-linked immunosorbent assay was used to detect the expression of inflammatory cytokines. Compared with healthy persons, the expression of Ripk1, NF-κB and IKBα in blood of TBI patients was significantly upregulated. After silencing of Ripk1 or inhibition of the NF-κB signaling pathway, the expression of IL-1β, IL-6, TNF-α, Bax, and cleaved-caspase-3 was downregulated, and the expression of Bcl-2, ATG5, and LC3II/LC3I was upregulated. Furthermore, neuron injury and apoptosis were notably reduced and neuron autophagy increased significantly by Ripk1 downregulation or IKKα inhibitor. Ripk1 overexpression contributed to activation of NF-κB signaling pathway, whereby aggravating TBI-induced damage. Silencing Ripk1 suppresses TBI by inhibiting inflammation and promoting autophagy of neurons via inhibition of NF-κB signaling pathway.
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http://dx.doi.org/10.1007/s11010-020-03789-5DOI Listing
September 2020

Differentially Expressed MicroRNAs in Radioresistant and Radiosensitive Atypical Meningioma: A Clinical Study in Chinese Patients.

Front Oncol 2020 30;10:501. Epub 2020 Apr 30.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

For atypical meningiomas (AMs), the combination of gross total resection (GTR) and adjuvant radiotherapy (ART) is still a controversial therapeutic strategy to improve prognosis. This study analyzed the factors influencing the prognosis on AM patients treated with GTR + ART by investigating both clinical characteristics and the change in microRNA (miRNA) expression. Adult AM patients who were admitted to the Tiantan hospital from 2008 to 2015 and underwent GTR + ART were included. Patients who suffered recurrence within 3 years after operation were considered radioresistant, while the others were considered radiosensitive. Clinical characterizations were compared between these two groups. The microRNA (miRNA) expression was detected via miRNA microarray in 10 patients, five from the radiosensitive group and from the radioresistant group. A total of 55 cases were included in this study. No significant difference was found in the clinical characteristics (gender, age, tumor location, tumor size, peritumoral brain edema, and Ki-67 index) between radiosensitive and radioresistant patients. We found seven significantly upregulated miRNAs (miR-4286, miR-4695-5p, miR-6732-5p, miR-6855-5p, miR-7977, miR-6765-3p, miR-6787-5p) and seven significantly downregulated miRNAs (miR-1275, miR-30c-1-3p, miR-4449, miR-4539, miR-4684-3p, miR-6129, miR-6891-5p) in patients resistant to radiotherapy. The differentially expressed miRNAs were enriched mostly in the fatty acid metabolic pathways (hsa00061, hsa01212) and transforming growth factor beta signaling pathway (hsa04350). For AM patients treated with GTR + ART, the changes in miRNA expression discovered in this study may be a potential predictor of individual sensitivity to adjuvant radiotherapy. Further research is needed regarding the predictive power and mechanism by which these miRNAs influence prognosis.
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http://dx.doi.org/10.3389/fonc.2020.00501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203448PMC
April 2020

Verification of needle guidance accuracy in pelvic phantom using registered ultrasound and MRI images for intracavitary/interstitial gynecologic brachytherapy.

J Contemp Brachytherapy 2020 Apr 30;12(2):147-159. Epub 2020 Apr 30.

School of Mechanical Engineering, Tianjin University, Tianjin, China.

Purpose: In combined intracavitary/interstitial (IC/IS) gynecologic brachytherapy, trackers attached to interstitial needles of localize real-time needle trajectories, and intraoperative ultrasound (US) images provide updated anatomy information during needle insertions. To achieve an effective visualization and image guidance, real-time needle trajectories and US images can be unified in preoperative magnetic resonance imaging (MRI) image space together. This study evaluates the rigid registration accuracy between US images and MRI images as well as the registration accuracy between US images and real-time needle trajectories in a pelvic phantom.

Material And Methods: A method for US probe calibration and accomplished rigid registration between MRI images and US images was proposed. An IC/IS applicator was designed. Micro electromagnetic sensor to track and localize real-time needle trajectories in 3D MRI image space was used. Marker validation to test the accuracy of US probe calibration and pelvic phantom validation to test the registration accuracy between US images and MRI images was conducted as well as and pelvic phantom study to verify the registration accuracy between real-time needle trajectories and needle trajectories in registered US images.

Results: US probe calibration accuracy was 0.80 ±0.23 mm ( = 60). Registration accuracy between US images and MRI images were 1.01 ±0.22 mm in the axial plane ( = 60) and 1.14 ±0.20 mm in the sagittal plane ( = 24). Registration accuracy between real-time needle trajectories and needle trajectories in registered US images were 1.25 ±0.31 mm ( = 40) and 1.61 ±0.28 degrees ( = 5), respectively.

Conclusions: In this study, we showed that under ideal conditions, rigid registration between MRI images and US images obtained high accuracy for real-time image guidance. Additionally, registered US images provided accurate image guidance during visual needle insertion in IC/IS gynecologic brachytherapy to achieve a combination of effective visualization and image guidance.
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http://dx.doi.org/10.5114/jcb.2020.94583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207233PMC
April 2020

Profile of Immunoglobulin G and IgM Antibodies Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).

Clin Infect Dis 2020 11;71(16):2255-2258

The Third People's Hospital of Shenzhen, Southern University of Science and Technology, National Clinical Research Center for Infectious Diseases, Shenzhen, China.

We profiled the serological responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein and spike (S) glycoprotein. The majority of the patients developed robust antibody responses between 17 and 23 days after illness onset. Delayed, but stronger, antibody responses were observed in critical patients.
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http://dx.doi.org/10.1093/cid/ciaa489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197626PMC
November 2020

A CT-guided robotic needle puncture method for lung tumours with respiratory motion.

Phys Med 2020 May 18;73:48-56. Epub 2020 Apr 18.

School of Mechanical Engineering, Tianjin University, Tianjin 300350, China.

Purpose: Percutaneous interventions rely on needle puncture to deliver medical devices into lesions. For lung tumours, respiratory motion makes effective puncture procedures difficult to achieve. To address this issue, a needle puncture method considering respiration is proposed to improve the accuracy of lung puncture.

Methods: The accuracy of puncture is ensured by visualization and needle guidance. Dynamic visualization of the respiratory motion is developed for needle path planning based on four-dimensional computed tomography (4DCT) images. The rendered image is synchronized with the actual breathing by using respiratory signals. A robotic needle insertion strategy for velocity adjustment based on these respiratory signals is designed to guide the needle towards the moving tumour.

Results: The dynamic visualization was tested on multiple 4DCT datasets and achieved a frame rate of over 32 frames per second (FPS). A computer simulation was carried out to verify the feasibility of the needle insertion strategy. Needle puncture was performed on a phantom, and a mean accuracy of 1.34±0.18 mm was achieved.

Conclusions: In this paper, an efficient and robust method is proposed to improve the visualization and targeting of lung puncture, which reduces the impact of respiratory motion on the accuracy.
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http://dx.doi.org/10.1016/j.ejmp.2020.04.003DOI Listing
May 2020

Novel Charging-Optimized Cathode for a Fast and High-Capacity Zinc-Ion Battery.

ACS Appl Mater Interfaces 2020 Mar 20;12(9):10420-10427. Epub 2020 Feb 20.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.

A rechargeable aqueous zinc-ion battery (ZIB) is one of the attractive candidates for large-scale energy storage. Its further application relies on the exploitation of a high-capacity cathode and the understanding of an intrinsic energy storage mechanism. Herein, we report a novel layered KVO cathode material for the ZIB, adopting a strategy of charging first to extract part of K-ions from vanadate in initial few cycles, which creates more electrochemically active sites and lowers charge-transfer resistance of the ZIB system. As a result, a considerable specific capacity of 302.8 mA h g at 0.1 A g, as well as a remarkable cycling stability (92.3% capacity retention at 4 A g for 2000 cycles) and good rate capability, are achieved. Besides, the energy storage mechanism was studied by in situ X-ray diffraction, in situ Raman spectroscopy, X-ray photoelectron spectroscopy, and inductively coupled plasma mass spectroscopy. An irreversible K-ion deintercalation in the first charge process is proved. It is believed that this novel cathode material for the rechargeable aqueous ZIB and the optimizing strategy will shed light on developing next-generation large-scale energy storage devices.
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http://dx.doi.org/10.1021/acsami.9b21579DOI Listing
March 2020

Formation and Fate of Formaldehyde in Methanol-to-Hydrocarbon Reaction: In Situ Synchrotron Radiation Photoionization Mass Spectrometry Study.

Angew Chem Int Ed Engl 2020 Mar 3;59(12):4873-4878. Epub 2020 Feb 3.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, 230029, P. R. China.

HCHO has been confirmed as an active intermediate in the methanol-to-hydrocarbon (MTH) reaction, and is critical for interpreting the mechanisms of coke formation. Here, HCHO was detected and quantified during the MTH process over HSAPO-34 and HZSM-5 by in situ synchrotron radiation photoionization mass spectrometry. Compared with conventional methods, excellent time-resolved profiles were obtained to study the formation and fate of HCHO, and other products during the induction, steady-state reaction, and deactivation periods. Similar formation trends of HCHO and methane, and their close correlation in yields suggest that they are derived from disproportionation of methanol at acidic sites. In the presence of Y O , the amount of HCHO changes, affecting the hydrogen-transfer processes of olefins into aromatics and aromatics into cokes. The yield of HCHO affects the aromatic-based cycle and the formation of ethylene, indicating that ethylene is mainly formed from the aromatic-based cycle.
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http://dx.doi.org/10.1002/anie.201914953DOI Listing
March 2020

Classification of lung nodules based on CT images using squeeze-and-excitation network and aggregated residual transformations.

Radiol Med 2020 Apr 8;125(4):374-383. Epub 2020 Jan 8.

School of Mechanical Engineering, Tianjin University, Tianjin, 300350, China.

Lung cancer is pointed as a leading cause of cancer death worldwide. Early lung nodule diagnosis has great significance for treating lung cancer and increasing patient survival. In this paper, we present a novel method to classify the malignant from benign lung nodules based on CT images using squeeze-and-excitation network and aggregated residual transformations (SE-ResNeXt). The state-of-the-art SE-ResNeXt module, which integrates the advantages of SENet for feature recalibration and ResNeXt for feature reuse, has great ability in boosting feature discriminability on imaging pattern recognition. The method is evaluated on the public available LUng Nodule Analysis 2016 (LUNA16) database with 1004 (450 malignant and 554 benign) nodules, achieving an area under the receiver operating characteristic curve (AUC) of 0. 9563 and accuracy of 91.67%. The promising results demonstrate that our method has strong robustness in the classification of nodules. The method has the potential to help radiologists better interpret diagnostic data and differentiate the benign from malignant lung nodules on CT images in clinical practice. To our best knowledge, the effectiveness of SE-ResNeXt on lung nodule classification has not been extensively explored.
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http://dx.doi.org/10.1007/s11547-019-01130-9DOI Listing
April 2020

Intracranial synovial sarcoma: A clinical, radiological and pathological study of 16 cases.

Eur J Surg Oncol 2019 Dec 16;45(12):2379-2385. Epub 2019 Aug 16.

Department of Neurosurgery, China National Clinical Research Center for Neurological Diseases (NCRC-ND), Center of Brain Tumor, Beijing Institute for Brain Disorders, Beijing Key Laboratory of Brian Tumor, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, PR China. Electronic address:

Introduction: Synovial sarcoma (SS) is a tumor of unknown origin and is extremely rare in the central nervous system. Most studies on intracranial SS included only one or two cases. To better understand the disease, we review a series of primary intracranial SS.

Method And Materials: 16 primary intracranial SS in Tiantan Hospital during 2008-2017 were included. The clinical characteristics, including radiological and histological examination, operative records, and prognoses were reviewed.

Result: The case series included nine male and seven female patients with an average age of 23.8 years. Radiological results showed that the supratentorial region (81.25%) was the most common site of the brain involved. All patients were misdiagnosed as non-SS tumors. Gross total resection (GTR) was achieved in 12 cases (75.0%), and subtotal resection (STR) was achieved in 4 cases. All cases showed the characteristic SYT-SSX fusion gene, as detected by RT-PCR. The mean progression-free survival time (PFS) was 10.0 months and the mean overall survival time (OS) was 15.5 months. Multivariate analysis revealed that GTR and postoperative adjuvant radiotherapy were independent factors for PFS (HR = 6.143, 95% CI = 1.491-25.312; P = 0.012, HR = 6.143, 95% CI = 1.491-25.312; P = 0.012 respectively) and OS (HR = 9.000, 95% CI = 1.627-49.773; P = 0.012, HR = 0.017, 95% CI = 0.001-0.213; P = 0.002 respectively).

Conclusion: Intracranial SS were more frequently observed in the supratentorial region and in young patients without sex predilection. We recommend adjuvant radiation regardless of the extent of resection. More patients and longer follow-up periods were needed to further elucidate the biological features of intracranial SS.
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http://dx.doi.org/10.1016/j.ejso.2019.08.015DOI Listing
December 2019

Hybrid optimization based on non-coplanar needles for brachytherapy dose planning.

J Contemp Brachytherapy 2019 Jun 28;11(3):267-279. Epub 2019 Jun 28.

Department of Oncology, the Second Hospital of Tianjin Medical University, Tianjin China.

Purpose: An ideal dose distribution in a target is the ultimate goal of preoperative dose planning. Furthermore, avoiding vital organs or tissues such as blood vessels or bones during the puncture procedure is significant in low-dose-rate brachytherapy. The aim of this work is to develop a hybrid inverse optimization method based on non-coplanar needles to assist the physician during conformal dose planning, which cannot be properly achieved with a traditional coplanar template.

Material And Methods: The hybrid inverse optimization technique include two novel technologies: an inverse optimization algorithm and a dose volume histogram evaluation method. Brachytherapy treatment planning system was designed as an experimental platform. Left lung adenocarcinoma case was used to test the performance of the method in non-coplanar and coplanar needles, and malignant tumor of spine case was involved to test the practical application of this technique. In addition, the optimization time of every test was also recorded.

Results: The proposed method can achieve an ideal dose distribution, avoiding vital organs (bones). In the first experiment, 13 non-coplanar needles and 24 seeds were used to get an ideal dose distribution to cover the target, whereas 11 coplanar needles and 23 seeds were used to cover the same target. In the second experiment, the new method used 22 non-coplanar needles and 65 seeds to cover the target, while 63 seeds and 22 needles were used in the actual operation. In addition, the computation time of the hybrid inverse optimization method was 20.5 seconds in the tumor of 94.67 cm by using 22 needles, which was fast enough for clinical application.

Conclusions: The hybrid inverse optimization method achieved high conformity in the clinical practice. The non-coplanar needle can help to achieve a better dose distribution than the coplanar needle.
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http://dx.doi.org/10.5114/jcb.2019.86167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6701384PMC
June 2019

1D Carbon-Based Nanocomposites for Electrochemical Energy Storage.

Small 2019 Nov 13;15(48):e1902348. Epub 2019 Aug 13.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, Hubei, 430070, P. R. China.

Electrochemical energy storage (EES) devices have attracted immense research interests as an effective technology for utilizing renewable energy. 1D carbon-based nanostructures are recognized as highly promising materials for EES application, combining the advantages of functional 1D nanostructures and carbon nanomaterials. Here, the recent advances of 1D carbon-based nanomaterials for electrochemical storage devices are considered. First, the different categories of 1D carbon-based nanocomposites, namely, 1D carbon-embedded, carbon-coated, carbon-encapsulated, and carbon-supported nanostructures, and the different synthesis methods are described. Next, the practical applications and optimization effects in electrochemical energy storage devices including Li-ion batteries, Na-ion batteries, Li-S batteries, and supercapacitors are presented. After that, the advanced in situ detection techniques that can be used to investigate the fundamental mechanisms and predict optimization of 1D carbon-based nanocomposites are discussed. Finally, an outlook for the development trend of 1D carbon-based nanocomposites for EES is provided.
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http://dx.doi.org/10.1002/smll.201902348DOI Listing
November 2019

Natural Killer Cells Offer Differential Protection From Leukemia in Chinese Southern Han.

Front Immunol 2019 16;10:1646. Epub 2019 Jul 16.

Division of Biomedical Informatics and Personalized Medicine, Department of Microbiology and Immunology, University of Colorado Anschutz Medical Campus, Aurora, CO, United States.

Interactions of human natural killer (NK) cell inhibitory receptors with polymorphic HLA-A, -B and -C molecules educate NK cells for immune surveillance against tumor cells. The haplotype encodes a distinctive set of HLA-specific NK cell inhibiting receptors having strong influence on immunity. We observed higher frequency of homozygosity among 745 healthy Chinese Southern Han than 836 adult patients representing three types of leukemia: ALL (OR = 0.68, 95% CI = 0.52-0.89, = 0.004), AML (OR = 0.76, 95% CI = 0.59-0.98, = 0.034), and CML (OR = 0.72 95% CI = 0.51-1.0, ns). We observed the same trend for NHL (OR = 0.47 95% CI = 0.26-0.88 = 0.017). For ALL, the protective effect of the genotype was greater in the presence of KIR ligands C1 (Pc = 0.01) and Bw4 (Pc = 0.001), which are tightly linked in East Asians. By contrast, the C2 ligand strengthened protection from CML (Pc = 0.004). NK cells isolated from individuals were significantly more cytotoxic toward leukemic cells than those from other genotypes ( < 0.0001). These data suggest KIR allotypes encoded by East Asian haplotypes are strongly inhibitory, arming NK cells to respond to leukemogenic cells having altered HLA expression. Thus, the study of populations with distinct and distributions enlightens understanding of immune mechanisms that significantly impact leukemia pathogenesis.
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http://dx.doi.org/10.3389/fimmu.2019.01646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6646668PMC
October 2020

Application of assisted portal under anterior horn of lateral meniscus for the treatment of discoid meniscus injury.

Knee 2019 Oct 24;26(5):1125-1135. Epub 2019 Jul 24.

Department of Orthopedics, Changhai Hospital Affiliated to Second Military Medical University, Shanghai, China. Electronic address:

Purpose: The assisted inferior anterolateral portal under anterior horn of the lateral meniscus (UAHLM portal) was applied to treat the lateral discoid meniscus injury conveniently and the clinical outcomes were evaluated.

Methods: A retrospective review was conducted on 60 patients who underwent arthroscopic surgery with a symptomatic discoid lateral meniscus. Normal anterolateral/anteromedial portals assisted with UAHLM portal (1-2 cm inferior to the anterolateral portal) were used. All patients were followed up for 24-48 months (median, 33 months) and evaluated by MRI images and clinical outcomes including clinical findings, Lysholm scores and IKDC scores.

Results: After meniscus plasty with or without repair, most of the upper layer of lateral meniscuses was retained. A total of 54 patients (16 males and 38 females, 42 ± 17.8 years old) showed satisfactory clinical results without requiring reoperation after a median follow-up time of 33 months. At final follow-up, a full range of motion was achieved in all patients. MRI indicated the thickness of anterior horn of lateral meniscus was (5.38 ± 1.09 mm) before the operation and (4.04 ± 0.71 mm) after the operation at the 2-year follow-up; clinical outcomes were improved significantly than the baseline: positive McMurray test (50 vs. 2, P< 0.001), Lysholm score (64.9 ± 9.0 vs. 94.7 ± 4.9, P< 0.001), and IKDC score (54.4 ± 7.7 vs. 92.6 ± 4.3, P< 0.001). No significant complication was observed during the follow-up.

Conclusion: Thus, this technique with assisted UAHLM portal was convenient for arthroscopic discoid meniscus plasty and meniscus repair and served as an effective method in patients with a symptomatic discoid lateral.
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http://dx.doi.org/10.1016/j.knee.2019.07.003DOI Listing
October 2019

Electron-Proton Co-doping-Induced Metal-Insulator Transition in VO Film via Surface Self-Assembled l-Ascorbic Acid Molecules.

Angew Chem Int Ed Engl 2019 Sep 23;58(39):13711-13716. Epub 2019 Aug 23.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, 230029, China.

Charge doping is an effective way to induce the metal-insulator transition (MIT) in correlated materials for many important utilizations, which is however practically limited by problem of low stability. An electron-proton co-doping mechanism is used to achieve pronounced phase modulation of monoclinic vanadium dioxide (VO ) at room temperature. Using l-ascorbic acid (AA) solution to treat VO , the ionized AA species donate electrons to the adsorbed VO surface. Charges then electrostatically attract surrounding protons to penetrate, and eventually results in stable hydrogen-doped metallic VO . The variations of electronic structures, especially the electron occupancy of V 3d/O 2p hybrid orbitals, were examined by synchrotron characterizations and first-principle theoretical simulations. The adsorbed molecules protect hydrogen dopants from escaping out of lattice and thereby stabilize the metallic phase for VO .
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http://dx.doi.org/10.1002/anie.201904148DOI Listing
September 2019

Identification of Gli1-interacting proteins during simvastatin-stimulated osteogenic differentiation of bone marrow mesenchymal stem cells.

J Cell Biochem 2019 11 27;120(11):18979-18994. Epub 2019 Jun 27.

Medical Research Center, North China University of Science and Technology, Tangshan, China.

Simvastatin has been shown to promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Our study aimed to illuminate the underlying mechanism, with a specific focus on the role of Hedgehog signaling in this process. BMSCs cultured with or without 10  mol/L simvastatin were subjected to evaluation of osteogenic differentiation capacity. Osteogenic markers such as type 1 collagen (COL1) and osteocalcin (OCN), as well as key molecules of Hedgehog signaling molecules, were examined by Western blot and real-time polymerase chain reaction (PCR). Co-immunoprecipitation and mass spectrometry assays were applied to screen for Gli1-interacting proteins. Cyclopamine (Cpn) was used as a Hedgehog signaling inhibitor. Our results indicated that simvastatin increased alkaline phosphatase (ALP) activity; mineralization of extracellular matrix; mRNA expression of ALP, COL1, and OCN; and expression and nuclear translocation of Gli1. Contrasting effects were observed in Cpn-exposed groups, but were partially rescued by the simvastatin treatment. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that Gli1-interacting proteins were primarily associated with mitogen-activated protein kinase (MAPK) (P = 7.04E ), hippo, insulin, and glucagon signaling. Further, hub genes identified by protein-protein interaction network analysis included Gli1-interacting proteins such as Ppp2r1a, Rac1, Etf1, and XPO1/CRM1. In summary, the current study showed that the mechanism by which simvastatin stimulates osteogenic differentiation of BMSCs involves activation of Hedgehog signaling, as indicated by interactions with Gli1 and, most notably, the MAPK signaling pathway.
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http://dx.doi.org/10.1002/jcb.29221DOI Listing
November 2019
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