Publications by authors named "Guo-Gang Li"

27 Publications

  • Page 1 of 1

Molecular phylogeny of the genus with special emphasis on the phylogenetic position of .

Zool Res 2021 Mar;42(2):212-216

Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences & Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303, China.

Muntjac deer (Cervidae: ) are often cited as an excellent model for the study of vertebrate evolution due to their fast rate of change in chromosome number among vertebrates. However, the phylogenetic relationships within generally, and the taxonomic status of specifically, remain unclear. Here, the phylogenetic relationships within were studied using mitochondrial genome (mitogenome) and cytochrome (cyt ) segments. Our results recognize 12 species within and support the controversial species , , and . Furthermore, Bayesian inference (BI) and maximum-likelihood (ML) approaches revealed and to be closely related species, with as their sister species, and and to be closely related, with as their sister species. The distribution range of was also confirmed in southwest China (Namdapha, Modong, Zayu and Gongshan) and northern Myanmar (Putao). The results of this study provide insight into the evolution of and further provide a molecular basis for the taxonomic evaluation of the genus in the future and fundamental data for the conservation of
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http://dx.doi.org/10.24272/j.issn.2095-8137.2020.355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995284PMC
March 2021

A new species of (Anura: Ranidae) from northern Myanmar.

Zool Res 2020 11;41(6):734-740

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, Guangxi 541004, China.

A new species of the genus , , is described from northern Myanmar. The new species can be distinguished from its congeners by the following characters: (1) dorsolateral fold distinct; (2) upper-lip stripe white; (3) male body size 37.6-40.2 mm; (4) ground color of dorsal surface brown, flank green, small warts on dorsum; (5) two internal subgular vocal sacs present; (6) HL slightly shorter than HW; (7) two palmar tubercles present, supernumerary tubercles and outer metatarsal tubercle absent; (8) tympanum smaller than half of eye diameter; (9) vomerine teeth present; (10) tibiotarsal articulation reaching beyond snout tip; (11) supratympanic fold indistinct; (12) pineal body present; (13) finger webbing absent, presence of circummarginal groove on tip of first finger; (14) nuptial pads present. The population from Myanmar represented a distinct maternal lineage within the group and was recovered as a sister taxon to with strong support (100) based on concatenated data. Average uncorrected pairwise distances ( -distances) between the specimens from Myanmar and other species in the genus ranged from 2.69% (vs. ) to 12.24% (vs. ) for 16S rRNA, 6.14% (vs. ) to 15.79% (vs. ) for , and 9.66% (vs. ) to 19.52% (vs. ) for
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http://dx.doi.org/10.24272/j.issn.2095-8137.2020.125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671909PMC
November 2020

Cognitive impairment and associated risk factors in older adult hemodialysis patients: a cross-sectional survey.

Sci Rep 2020 07 27;10(1):12542. Epub 2020 Jul 27.

Department of Nephrology, Chinese PLA General Hospital, State Key Laboratory of Kidney Disease, Beijing, 100853, China.

The clinical epidemiological features of cognitive impairment in Chinese older adult patients undergoing hemodialysis are not clear, we aimed to identify the extent and patterns of cognitive impairment among those patients. We conducted a cross-sectional study of 613 hemodialysis patients aged 50 to 80 from 11 centers in Beijing. A neuropsychological battery of 11 tests covering domains of attention/processing speed, executive function, memory, language, and visuospatial function was applied, patients were classified as none, mild, or major cognitive impairment according to the fifth version of the Diagnostic and Statistical Manual of Mental Disorders criteria for cognitive impairment. Compared with Chinese population norms, 37.2% of the participants had mild cognitive impairment, 43.7% had major cognitive impairment. Memory and language were the most severe impaired domains in the mild cognitive impairment group, attention and visuospatial function domains were the most serious impaired domains in the major cognitive impairment group. Concomitant impairment across multiple cognitive domains was common. Factors associated with major cognitive impairment included age, education level, history of stroke and hypertension, dialysis vintage, and single-pool Kt/V. There is a high frequency of cognitive impairment in Chinese older adult hemodialysis patients, with varying severity and concomitant impairment across multiple domains.
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http://dx.doi.org/10.1038/s41598-020-69482-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385128PMC
July 2020

New country records for three species of frog from Myanmar including two genera (Nasutixalus and Oreolalax).

Zootaxa 2020 Feb 21;4742(3):zootaxa.4742.3.7. Epub 2020 Feb 21.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650204, China.

Myanmar, a biodiversity hotspot, harbors a striking diversity and endemism of species. Despite this, its herpetofauna remains one of the least explored in continental Asia due to restrictions of crossing political boundaries and infrastructure in remote regions. Many species in adjacent China and India are hypothesized to occur in Myanmar but records are wanting. Recent fieldwork found the frogs Polypedates braueri, Nasutixalus jerdonii and Oreolalax jingdongensis there, and the latter two species represent new generic records for Myanmar. All major morphological characters of these populations match the original descriptions. In addition, our matrilineal genealogy based on DNA barcoding confirms their identities. Overall, these findings confirm that the amphibian diversity is underestimated and this has important implications for conservation. Analyses indicate that northern Myanmar is a biogeographic corridor for the Himalayas, southern China, and northeastern India.
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http://dx.doi.org/10.11646/zootaxa.4742.3.7DOI Listing
February 2020

Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China.

J Infect Dev Ctries 2019 12 31;13(12):1095-1100. Epub 2019 Dec 31.

Department of Clinical Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, China.

Introduction: Non-tuberculous mycobacterium (NTM) can colonize the human body, leading to opportunistic infection. This study was conducted to analyze the NTM species composition in a primary hospital and investigate the potential features of the patients with different NTM species.

Methodology: Mycobacterial strains were collected from the patients admitted at the hospital from January 2016 to May 2019. MPB64 assay was used to screen NTM strains and confirmed by Rv0577 amplification. The species were identified by hsp65 sequencing. The clinical records of patients with NTM were retrospectively reviewed.

Results: Among the 122 identified NTM isolates, the most common strains were Mycobacterium avium complex (MAC, n = 102, 83.6%), Mycobacterium abscessus (n = 9, 7.4%) and Mycobacterium lentiflavum (n = 5, 4.1%). The predominant species among MAC were Mycobacterium chimaera (n = 57, 46.7%), followed by Mycobacterium intracellulare (n = 25, 20.5%) and Mycobacterium colombiense (n = 17, 13.9%). A significantly lower percentage of positive acid-fast assay was observed in Mycobacterium colombiense positive patients than in those with Mycobacterium intracellulare and Mycobacterium chimaera. Mycobacterium intracellulare was more frequently isolated in patients from the infectious department than in other MAC members.

Conclusions: A predominant prevalence of Mycobacterium chimaera in Dongyang of Zhejiang Province was different from other regions in China, indicating that its prevalence has been likely underestimated. The heterogeneity in clinical features, caused by different MAC members, required an accurate species identification of the NTM isolated in the primary hospitals.
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http://dx.doi.org/10.3855/jidc.11772DOI Listing
December 2019

Complete mitochondrial genome of Gongshan muntjac (), a Critically Endangered deer species.

Mitochondrial DNA B Resour 2019 Sep 6;4(2):2867-2868. Epub 2019 Sep 6.

Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Yezin, Nay Pyi Taw, Myanmar and Center for Integrative Garden, Chinese Academy of Sciences, Mengla, Yunnan, China.

The first complete mitochondrial genome of the Gongshan muntjac (s) was determined and annotated (GenBank accession nos. MK882935). The 16,356 bp circular genome contained 13 protein-coding genes (PGCs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 1 control region (D-loop). Phylogenetic analysis revealed that Gongshan muntjac is most closely related to Black muntjac (), with Fea's muntjac () as their sister species. These data will be useful for further studies on the genetic diversity and molecular phylogenetic relationship of the genus .
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http://dx.doi.org/10.1080/23802359.2019.1660242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707006PMC
September 2019

The First Report of Laparoscopic Pancreaticoduodenectomy for Primary Duodenal Carcinoma in a Patient With Situs Inversus Totalis: Report of a Rare Case.

Surg Laparosc Endosc Percutan Tech 2019 Jun;29(3):e29-e33

Departments of Hepatobiliary and Pancreatic Surgery.

Background: Situs inversus (SI) is a rare congenital anomaly characterized by the transposition of thoracic and abdominal viscera. Laparoscopic pancreaticoduodenectomy (LPD) is increasingly used in patients with periampullar and pancreatic carcinomas. For patients with SI, LPD can be more complicated because of reversed anatomy and possible other associated anomalies that have not been expected before surgery.

Case Presentation: A female patient with SI totalis presented with inappetence, vomiting, and weight loss for 2 months. Imaging modalities and angiography revealed a mass in the periampullary region without obvious vascular abnormalities. The mass was successfully resected via LPD based on an elaborate preoperative plan. The surgical pathology report demonstrated adenocarcinoma of the duodenal papilla. The patient has been followed up for 4 months and no tumor recurrence or long-term complications were observed.

Conclusion: LPD is technically difficult but feasible in patients with SI.
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http://dx.doi.org/10.1097/SLE.0000000000000659DOI Listing
June 2019

A preoperative nomogram predicts prognosis of up front resectable patients with pancreatic head cancer and suspected venous invasion.

HPB (Oxford) 2018 11 19;20(11):1034-1043. Epub 2018 Jun 19.

Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, Hangzhou, China. Electronic address:

Background: Pancreatic head adenocarcinoma is commonly diagnosed at an advanced stage when adjacent vascular invasion is present. This study aimed to establish a preoperative prognostic nomogram for patients who underwent attempted curative resectional surgery for pancreatic head cancer with suspected peripancreatic venous invasion.

Methods: Data on all consecutive patients were retrospectively collected from 2012 to 2016 at four academic institutions. The demographic and radiological parameters were analyzed using univariate and multivariate Cox regression analyses. The final nomogram was established using the concordance Harrell's C-indices and calibration curves from data obtained in three institutions and validated in the cohort of patients coming from the fourth institution.

Results: The nomogram was constructed using data from 178 patients while the validation cohort consisted of 61 patients. Age, length of tumor contact, peripancreatic venous abnormalities and lymph node staging were independent factors of overall survival. The nomogram showed good probabilities of survival on calibration curves. The C-index of the model in predicting overall survival (OS) was 0.824 for the validation cohort.

Conclusions: The nomogram accurately predicted OS in patients with pancreatic head cancer with suspected peripancreatic venous invasion after attempted curative pancreatic resectional surgery.
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http://dx.doi.org/10.1016/j.hpb.2018.04.010DOI Listing
November 2018

Complete mitochondrial genome of the leaf muntjac () and phylogenetics of the genus .

Zool Res 2017 Sep;38(5):310-316

Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Yezin Nay Pyi Taw 05282, Myanmar.

The leaf muntjac () is an endemic deer species found in the east trans-Himalayan region. In recent years, population numbers have decreased due to heavy hunting and habitat loss, and little genetic data exists for this species, thus our knowledge of distribution rangs and population sizes likewise remain limited. We obtained mtDNA genes and the complete mitochondrial genome sequence of using PCR, followed by direct sequencing. The complete mitogenome sequence was determined as a circular 16 349 bp mitochondrial genome, containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and one control region, the gene composition and order of which were similar to most other vertebrates so far reported. Most mitochondrial genes, except for and eight tRNAs, were encoded on the heavy strand. The overall base composition of the heavy strand was 33.1% A, 29.3% T, 24.2% C, and 13.4% G, with a strong AT bias of 62.4%. There were seven regions of gene overlap totaling 95 bp and 11 intergenic spacer regions totaling 74 bp. Phylogenetic analyses (ML and BI) among the genus based on the sequenced of mitogenome and supported as a member of , most closely related to . However, when analyses based on cyt included two more muntjacs, was most closely related to rather than , and together with , likely forming a complex of the species. This study will help in the exploration of the evolutionary history and taxonomic status of the leaf muntjac, as well as its protection as a genetic resource.
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http://dx.doi.org/10.24272/j.issn.2095-8137.2017.058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717431PMC
September 2017

Bird diversity in northern Myanmar and conservation implications.

Zool Res 2017 Sep;38(5):264-280

Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Yezin Nay Pyi Taw 05282, Myanmar.

We conducted four bird biodiversity surveys in the Putao area of northern Myanmar from 2015 to 2017. Combined with anecdotal information collected between 2012 and 2015, we recorded 319 bird species, including two species ( and ) previously unrecorded in Myanmar. Bulbuls (Pycnonotidae), babblers (Timaliidae), pigeons and doves (Columbidae), and pheasants and partridges (Phasianidae) were the most abundant groups of birds recorded. Species richness below 1 500 m a. s. l. was higher than species richness at higher elevations. Our results suggest that the current protected areas in this region should be expanded to lower elevations to cover critical conservation gaps.
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http://dx.doi.org/10.24272/j.issn.2095-8137.2017.059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717427PMC
September 2017

Modified-FOLFIRINOX in metastatic pancreatic cancer: A prospective study in Chinese population.

Cancer Lett 2017 10 17;406:22-26. Epub 2017 Jul 17.

Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, Hangzhou, China. Electronic address:

FOLFIRINOX chemotherapy has shown remarkable responses in patients with metastatic pancreatic cancer (MPC), and has significantly improved prognosis. However, FOLFIRINOX is currently not frequently applied in China because of its high incidence of adverse events, and there is no recognized optimization for this therapy in Chinese population. Modification of FOLFIRINOX may be better for its acceptance in China. In this study, we evaluated the efficacy and safety of modified-FOLFIRINOX in patients with MPC. A total of 62 MPC patients were treated with modified-FOLFIRINOX (no Fluorouracil bolus, 85% Oxaliplatin and 75% Irinotecan) between April 2014 and April 2017 in our institute. 40 of them were evaluated, with a response rate of 32.5% (13/40). The frequent grade 3/4 adverse events are neutropenia (29%) and alanine aminotransferase elevation (14.5%). No treatment-related death was observed. The median overall survival and median progression-free survival are 10.3 months and 7.0 months, respectively. In conclusion, modified-FOLFIRINOX had significantly improved tolerance with similar efficacy to FOLFIRINOX. These findings may provide evidence for the use of FOLFIRINOX in Chinese patients with MPC.
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http://dx.doi.org/10.1016/j.canlet.2017.07.012DOI Listing
October 2017

Taxonomic status of a population of Gymoncypris waddelli Regan, 1905 (Cypriniformes: Schizothoracinae) distributed in Pengqu River, Tibet, China.

Zootaxa 2016 Jun 16;4126(1):123-37. Epub 2016 Jun 16.

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China Laboratory of Plateau Fish Evolutionary and Functional Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China; Email:

Gymnocypris waddelli, a highland cold-water fish, is distributed among rivers and lakes of the southern Qinghai-Tibet Plateau. Although previously described as G. waddelli, specimens from the Pengqu River are morphologically distinguishable from those from Lake Yamzhog Yumco, which is the type locality of G. waddelli. We investigated morphological variations of G. waddelli specimens from Pengqu River and Lake Yamzhog Yumco using not only traditional morphological methods but also newly-developed geometric morphometric analysis; furthermore, mitochondrial Cytochrome b (Cyt b) gene analysis was employed to explore the phylogenetic position of the Pengqu River population. Our morphological analyses suggested that G. waddelli from the Pengqu River had an obtuse snout, nearly straight oral fissure, shorter lower jaw than upper jaw, broad mouth and sparse inside gill rakers of the first arch relative to the Lake Yamzhog Yumco population. The strongly supported monophyly of Pengqu population according to mitochondrial datasets was obtained, indicating genetic differences between Pengqu River and Lake Yamzhog Yumco populations. Given their substantial genetic and morphological divergence, specimens from Pengqu River warrant recognition as a distinct species, here described and named Gymnocypris pengquensis.
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http://dx.doi.org/10.11646/zootaxa.4126.1.7DOI Listing
June 2016

Hypoxia-Induced Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma Induces an Immunosuppressive Tumor Microenvironment to Promote Metastasis.

Cancer Res 2016 Feb 2;76(4):818-30. Epub 2016 Feb 2.

Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. China. Key Laboratory of Cancer Prevention and Intervention, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, P.R. China Collaborative Innovation Center for Cancer Medicine, Zhejiang University, Hangzhou, P.R. China.

Portal vein tumor thrombosis (PVTT) is a significant risk factor for metastasis in hepatocellular carcinoma (HCC) patients and is therefore associated with poor prognosis. The presence of PVTT frequently accompanies substantial hypoxia within the tumor microenvironment, which is suggested to accelerate tumor metastasis, but it is unclear how this occurs. Recent evidence has shown that the hypoxia-inducible factor HIF-1α induces epithelial-to-mesenchymal transition (EMT) in tumor cells to facilitate metastasis. In this study, we investigated whether hypoxia-induced EMT in cancer cells also affects immune cells in the tumor microenvironment to promote immunosuppression. We found that hypoxia-induced EMT increased the expression of the CCL20 cytokine in hepatoma cells. Furthermore, coculture of monocyte-derived macrophages with hypoxic hepatoma cells revealed that the expression of indoleamine 2, 3-dioxygenase (IDO) was induced in monocyte-derived macrophages in a CCL20-dependent manner. In turn, these IDO-expressing monocyte-derived macrophages suppressed T-cell proliferation and promoted the expansion of immunosuppressive regulatory T cells. Moreover, high CCL20 expression in HCC specimens was associated with PVTT and poor patient survival. Collectively, our findings suggest that the HIF-1α/CCL20/IDO axis in hepatocellular carcinoma is important for accelerating tumor metastasis through both the induction of EMT and the establishment of an immunosuppressive tumor microenvironment, warranting further investigation into the therapeutic effects of blocking specific nodes of this signaling network.
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http://dx.doi.org/10.1158/0008-5472.CAN-15-0977DOI Listing
February 2016

LB-100 sensitizes hepatocellular carcinoma cells to the effects of sorafenib during hypoxia by activation of Smad3 phosphorylation.

Tumour Biol 2016 Jun 14;37(6):7277-86. Epub 2015 Dec 14.

Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88 Jiefang Road, Hangzhou, 310009, China.

Hepatocellular carcinoma (HCC) is a common cancer with poor prognosis. The multikinase inhibitor sorafenib is the only clinically proved systematic treatment for HCC. However, few patients respond to sorafenib. Hypoxic microenvironments contribute to sorafenib resistance. LB-100, a serine/threonine protein phosphatase 2A (PP2A) inhibitor was previously found to be a chemosensitizer in HCC. Here, we tested whether LB-100 could sensitize HCC to the effects of sorafenib. Intriguingly, LB-100 enhanced the effects of sorafenib in HCC cells only during hypoxic environments. LB-100 dramatically increased intracellular p-Smad3 level, which was responsible for the effect of LB-100 as a sensitizer. LB-100 downregulated Bcl-2 expression and enhanced sorafenib-induced apoptosis in HCC cells. We further proved that PP2A mediated LB-100-induced p-Smad3 overexpression. In addition, p38 mitogen-activated protein kinase pathway was activated in hypoxic conditions, and enhanced p-Smad3-dependent Bcl-2 inhibition and consequent apoptosis. In conclusion, LB-100 sensitized HCC cells to sorafenib in hypoxic environments. This effect was mediated by inactivation of PP2A, resulting in enhanced level of p-Smad3. Increased p-Smad3 downregulated Bcl-2, causing increased apoptosis of HCC cells.
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http://dx.doi.org/10.1007/s13277-015-4560-2DOI Listing
June 2016

CD97 promotes gastric cancer cell proliferation and invasion through exosome-mediated MAPK signaling pathway.

World J Gastroenterol 2015 May;21(20):6215-28

Chao Li, Da-Ren Liu, Guo-Gang Li, Hou-Hong Wang, Xiao-Wen Li, Yu-Lian Wu, Li Chen, Department of Surgery, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310021, Zhejiang Province, China.

Aim: To investigate the mechanism underlying the promoting role of CD97 in gastric cancer cell proliferation and invasion.

Methods: Two types of exosomes released by gastric cancer cells with high (SGC/wt) or low (SGC/kd) CD97 expression were isolated by ultracentrifugation and identified by electron microscopy and western blot analysis. The influences of the two exosomes on gastric cancer cell proliferation and invasion were investigated by proliferation and Matrigel invasion assays. Exosomal miRNAs were subsequently isolated from the two samples and their miRNA profiles were compared via microarray assay analysis. Reverse transcription-quantitative real-time polymerase chain reaction was used to validate the microarray assay. Target genes of the differently expressed microRNAs were predicted based on five independent algorithms and were then subjected to gene oncology enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. After identifying the pathway that was the most likely altered, tumor cells were treated with the two exosomes at different concentrations, and the pathway activation was identified through western blot analysis.

Results: Exosomes isolated from SGC/wt cells significantly promoted tumor cell proliferation in a dose-dependent manner in vitro. SGC/wt exosomes also significantly elevated the invasiveness of both SGC/wt (129.67 ± 8.327 vs 76.00 ± 5.292, P < 0.001) and SGC/kd (114.52 ± 9.814 vs 45.73 ± 4.835, P < 0.001) cells as compared to the exosomes released by SGC/kd cells. Microarray assay of the two exosomes revealed that 62 miRNAs were differently regulated with a signal intensity of > 500 and a false discovery rate < 0.05. The following KEGG analysis defined the MAPK signaling pathway as the most likely candidate pathway that regulated tumor cell proliferation and invasion. Through western blot analysis, significant up-regulations of phosphorylated MAPKs, including extracellular signal-regulated kinase, Jun NH2-terminal kinase, and p38 mitogen-activated protein kinase, were detected in a dose-dependent manner in the SGC/wt exosomes treated groups, confirming activation of the MAPK signaling pathway stimulated by SGC/wt exosomes.

Conclusion: CD97 promotes gastric cancer cell proliferation and invasion in vitro through exosome-mediated MAPK signaling pathway, and exosomal miRNAs are probably involved in activation of the CD97-associated pathway.
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http://dx.doi.org/10.3748/wjg.v21.i20.6215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4445098PMC
May 2015

Association between circulating endothelial cells and carotid atherosclerosis in patients receiving maintenance hemodialysis.

Biomed Res Int 2014 20;2014:753759. Epub 2014 Mar 20.

Department of Nephrology, Fuxing Hospital, Capital University of Medical Science, Beijing, China.

Accelerated atherosclerosis is the major cause of mortality in maintenance hemodialysis (MHD) patients, and endothelial injury associated with MHD might contribute strongly to pathogenesis. The current study was designed to explore possible associations between circulating endothelial cells (CECs) and intima-media thickness of common carotid artery (CCA-IMT) as an indicator of carotid atherosclerosis. Sixty-two MHD patients and 26 age- and sex-matched healthy volunteers were recruited. The number of CECs was determined in peripheral blood using multiparametric flow cytometry. CCA-IMT and presence of plaques in the common carotid arteries were assessed with ultrasound. Laboratory tests results and the demographics were recorded. The finding indicated that numbers of CECs were higher in patients before hemodialysis (predialysis) compared with numbers in controls (P = 0.045). CCA-IMT was also significantly higher in patients than in controls (P < 0.01). A positive relationship was observed between predialysis CECs numbers and CCA-IMT (P < 0.01) in MHD patients. In multiple linear regression analysis, the relationship between the predialysis CECs level and CCA-IMT remained the same even if adjusting for confounding effects. Accordingly, the investigation indicates that the CECs level is positively associated with CCA-IMT in our hemodialysis patients. CECs might be an important marker to the severity of carotid atherosclerosis in MHD patients.
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http://dx.doi.org/10.1155/2014/753759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977501PMC
November 2014

[Morphological differentiations of the gills of two Gymnocypris przewa-lskii subspecies in different habitats and their functional adaptations].

Dongwuxue Yanjiu 2013 Aug;34(4):387-91

Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.

Gill morphologies of two subspecies of Gymnocypris przewalskii (Gymnocypris przewalskii przewalskii and Gymnocypris przewalskii ganzihonensis) in different habitats were analyzed under scanning electron microscope. Results indicated that G. p. przewalskii had numerous long and dense-lined gill rakers while G. p. ganzihonensis had few short and scatter-lined gill rakers. There were no significant differences in distance between gill filaments (DBF) and distance gill lamella (DBL) between the two subspecies, but gill filaments of G. p. przewalskii were longer than in G. p. ganzihonensis. The electron microscopic study indicated that the pavement epithelium cells of G. p. przewalskii were well defined as irregular ovals, but were hexagonal in G. p. ganzihonensis. Moreover, G. p. przewalskii had more chloride cells than G. p. ganzihonensis, and mucous cells were only found on the surface of gill filaments of G. p. przewalskii. The morphological differences between the two subspecies of G. przewalskii are adaptations to their corresponding diets and habitats.
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http://dx.doi.org/10.11813/j.issn.0254-5853.2013.4.0387DOI Listing
August 2013

Influence of up-regulation of Notch ligand DLL4 on biological behaviors of human gastric cancer cells.

World J Gastroenterol 2013 Jul;19(28):4486-94

Department of Surgery, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China.

Aim: To investigate the potential roles of Delta-like ligand 4 (DLL4) on the biological behavior of gastric cancer cells and its molecular mechanisms.

Methods: A recombinant eukaryotic expression vector containing human DLL4 gene was constructed and transfected into the human gastric cancer cell line SGC7901. Clones with up-regulated DLL4 were selected and amplified. The effect of DLL4 up-regulation on gastric cancer cell growth was assessed using cell growth assay. The migration and invasion were assessed using a transwell migration assay and matrigel invasion assay. Matrix metalloproteinases were detected using the zymogram technique. Cells were implanted subcutaneously into male BALB/c nu/nu mice. Tumor volumes were then calculated and compared. DLL4 staining in the implanted tumor was performed using immunohistochemistry technique.

Results: Growth curves over a six-day time course showed significantly promoted cell proliferation of SGC7901 cells with up-regulated DLL4. DLL4 up-regulation in SGC7901 cells promoted the migration (205.4 ± 15.2 vs 22.3 ± 12.1, P < 0.05) and invasion (68.8 ± 5.3 vs 18.2 ± 6.0, P < 0.05) in vitro and tumorigenicity in vivo (2640.5 ± 923.6 mm(3) vs 1115.1 ± 223.8 mm(3), P < 0.05). Furthermore, significantly increased mRNA level and increased secretion of matrix metalloproteinase-2 (MMP-2) proenzyme were observed in SGC7901 cells with up-regulated DLL4. However, increased MMP-9 mRNA level but decreased extracellular MMP-9 proenzyme level was observed.

Conclusion: Our observations indicated a mechanism by which activation of DLL4-mediated Notch signaling promotes the expression and secretion of MMP-2 proenzyme and influences the progress of gastric cancer.
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http://dx.doi.org/10.3748/wjg.v19.i28.4486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725372PMC
July 2013

[Diagnosis and treatment of parathyroid carcinoma: 9 cases report and literature review].

Zhonghua Wai Ke Za Zhi 2010 Jun;48(12):886-90

Department of Surgery, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310009, China.

Objective: To investigate the diagnosis and surgical treatment of parathyroid carcinoma.

Methods: The clinical data of 9 cases of parathyroid carcinoma treated from January 1967 to December 2009 was analyzed retrospectively with the review of related Chinese literatures.

Results: Parathyroid carcinoma accounted for 8.9% (8/90) of all patients with primary hyperparathyroidism in our hospital, and the other one case was transferred from another hospital. Of the patients, 8 cases were found with primary hyperparathyroidism. Primary surgery was carried out with small incision: 5 patients underwent en bloc resection, among which, 3 cases received central lymph node dissection; 2 patients received simple parathyroidectomy; one case underwent palliative tumor resection. The case from another hospital received subtotal thyroidectomy. Considering preoperative, intraoperative data and frozen sections pathology, all patients were diagnosed as parathyroid carcinoma. Nine patients were followed-up for 1 - 14 years, no recurrence occurred, and the patient received palliative resection died from carcinoma two years after the operation. In previous Chinese literatures and this group, there were total 146 patients reported as parathyroid carcinoma. Those patients were diagnosed through routine histopathology, accounted for 1.8% - 11.5% of patients with primary hyperparathyroidism.

Conclusions: The diagnosis of parathyroid carcinoma is established according to severe hypercalcemia, clinical features, subset B-ultrasound and Tc(99m)-sestamibi scanning, intraoperative finding of adherence to close structures and histopathology. The initial surgical procedure of choice is en bloc resection of the tumor by minimally invasive small incision, including adjacent structures and ipsilateral thyroidectomy. The prognosis is favorable after the operation.
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June 2010

[Quantitative analysis of phenanthrene and humic acid in solution based on fluorescence method].

Guang Pu Xue Yu Guang Pu Fen Xi 2009 Aug;29(8):2213-6

Key Laboratory of Environmental Optics & Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China.

Polycyclic aromatic hydrocarbons as a universal concern in the monitoring of priority pollutants have low content in the water environment and are interfered with the coexistence of humic acid. The spectra of humic acid and polycyclic aromatic hydrocarbons overlap seriously, so it was difficult to use the conventional methods for rapid quantitative detection. As a model compound of polycyclic aromatic hydrocarbons, Phenanthrene (PHE) was chosen in the experiment. The fluorescence spectra of PHE and humic acid (HA) were investigated by three dimensional fluorescence excitation-emission matrix. The method combined excitation-emission matrix fluorescence spectra with parallel factor analysis and was applied to determine PHE and HA directly. The excitation wavelength changed from 240 to 360 nm at an interval of 5 nm. The emission wavelength varied from 260 to 575 nm at an interval of 5 nm. The satisfactory results show that this experiment can be easily performed without paying out time-consuming and complicated procedures.
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August 2009

[Study on three-dimensional fluorescence spectra of phenanthrene].

Guang Pu Xue Yu Guang Pu Fen Xi 2009 May;29(5):1319-22

Key Laboratory of Environmental Optics & Technology, Anhui Institute of Optics and Fine Mechanics, The Chinese Academy of Sciences, Hefei 230031, China.

According to the high fluorescence quantum yields of polycyclic aromatic hydrocarbons (PAHs), the fluorescence spectra of phenanthrene were investigated by three dimensional fluorescence excitation-emission matrix (3DEEM). The results show that the three-dimensional fluorescence spectra of phenanthrene in aqueous solution mainly have two fluorescence peaks. On the basis of three-dimensional fluorescence spectrometry analysis of phenanthrene, the excitation wavelength of 255 nm and emission wavelength of 273 nm were chosen for the quantitative analysis of phenanthrene. The linear range for the determination of phenanthrene was 5.0-250.0 mg x mL(-1), its detection limit was 3. 88 ng x mL(-1), and its relative standard deviation was 4.23% (n=5). It was a good precision. It has been tested satisfactorily for the determination of artificial sample in tap water. The recoveries are in the range of 90%-105%. The method provided basis for the rapid monitoring of trace PAHs in water.
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May 2009

Spatial and temporal distribution of polycyclic aromatic hydrocarbons (PAHs) in sediments of the Nansi Lake, China.

Environ Monit Assess 2009 Jul 26;154(1-4):469-78. Epub 2009 Feb 26.

Shandong Environmental Monitoring Centre, 50 Lishan Road, Jinan, Shandong 250013, China.

Polycyclic aromatic hydrocarbons (PAHs) were measured in surface sediments and dated core sediments from the Nansi Lake of China to investigate the spatial and temporal distribution characteristics. The concentrations of 16 kinds priority PAH compounds were determined by GC-MS method. And (210)Pb isotope dating method was used to determine the chronological age of the sediment as well as the deposition rate. The results indicated that the total PAHs concentration ranges in surface and core sediment samples were 160 approximately 32,600 and 137 approximately 693 ng/g (dry wt.), respectively. The sediment rate and the average mass sedimentation were calculated to be 0.330 cm x year(-1) and 0.237 g x cm(-2) x yr(-1) and the sediment time of the collected core sample ranged from 1899 to 2000. The peak of PAH concentrations came at recent years. The source analysis showed PAHs mainly came from the contamination of low temperature pyrogenic processes, such as coal combustion. The PAHs concentrations were lower than ERL and LEL values for most collected samples. However, in several surface sediment samples especially in estuary sites, the PAHs concentrations were not only higher than ERL and LEL values, but also higher than ERM values.
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http://dx.doi.org/10.1007/s10661-009-0752-9DOI Listing
July 2009

[Clinical characteristics and laboratory assay of adult Japanese encephalitis patients in an outbreak in Yuncheng, Shanxi Province, 2006].

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2008 Apr;22(2):95-7

The Second People's Hospital of Yuncheng City, Shanxi Province, Taiyuan, China.

Objective: To study the clinical and laboratory characteristics of adult Japanese encephalitis (JE) patients in a JE outbreak in Yuncheng, Shanxi Province in 2006.

Method: All the clinical data from the Second People's Hospital in Yuncheng city were analyzed, part of patients' sera and cerebrospinal fluid were tested by serology and RT-PCR.

Results: The majority of patients were middle-aged and elderly, 77.8% (35/45) of the total cases were more than 40 years old. Severe and fulminating type cases accounted for 60.0% (27/45). Most patients had underlying diseases. IgM antibody to JE virus (JEV) in serum was positive in each of the 45 patients analyzed and 4-fold or greater rise in sera neutralization antibody titer were found in convalescent serum. JEV nucleic acid was positive in part of cerebrospinal fluid specimens.

Conclusion: Viral encephalitis emerged in Yuncheng city, Shanxi Province was Japanese encephalitis B, and most of the cases belonged to elderly group.
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April 2008

[Vertical distribution characteristics of organochlorine pesticides and polychlorinated biphenyls in sediment core from Lake Nansihu].

Huan Jing Ke Xue 2007 Jul;28(7):1590-4

Environmental Research Institute, Shandong University, Ji'nan 250100, China.

The organochlorinne pesticides (OCPs) and the polychlorinated biphenyls (PCBs) in sediment core collected from Lake Nansihu were quantificationally analyzed by GC-ECD. The pollution history of OCPs and PCBs in this area was reconstructed by analysis of the concentrations of OCPs and PCBs connected with the deposition time by 210Pb isotope dating method. The results indicated that the concentration ranges of OCPs, HCHs, DDTs and PCBs were 1.64 - 17.9, 0.66 - 12.5, 0.24 - 2.99 and 7.84 - 42.8 ng g(-1) respectively. The sediment rate and the average mass sedimentation were calculated to be 0.330 cm a(-1) and 0.237 g (cm2 a)(-1), and the sediment time of this core sample ranged from 1899 to 2000. OCPs and PCBs had the peak value at 1960s and 1970s. The source analysis showed HCH came from HCH products and lindane, and HCH and DDT came from historic using. The source of PCBs had been deduced to be the blench process of paper making and incinerator emission. Except the concentration of beta-HCH, gamma-HCH and aldrin at 14 - 16 cm depth, the other concentrations of OCPs and PCBs were all less than the low ecologic risk value. The whole sediment core of Lake Nansihu belonged to the low ecologic risk level.
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July 2007

[Study on diagnostic standard for dampness syndrome in patients with chronic gastritis].

Zhongguo Zhong Xi Yi Jie He Za Zhi 2005 Nov;25(11):975-9

Shanghai University of Traditional Chinese Medicine, Shanghai.

Objective: To consummate the standard of macroscopic syndrome differentiation for diagnosis of dampness syndrome in patients with chronic gastritis (CG), based on the essentials of syndrome differentiation in TCM diagnostics and combined with the multivariant mathematical statistic method. And try to find out the methods and approaches for establishing standard for TCM syndrome differentiation.

Methods: Clinical investigation on CG patients was carried out adopting clinical epidemiological method.

Results: The meaningful symptoms for making diagnosis of Pi-Wei damp-heat syndrome in frequency order, were red tongue with yellow and greasy fur, sticky and greasy sensation in mouth, brown urine, constipation, dry stool, flushed face and ponderous extremities. Those for Pi deficiency with damp retention syndrome were swollen tongue with teeth-print, greasy fur, sticky and greasy sensation in mouth, tastelessness, and poor appetite.

Conclusion: By combining the integrative medical theory with multivariant statistic method, the meaningful essentials for diagnosis of dampness syndrome can be screened out.
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November 2005

[Immunological mechanism of exfoliative tongue fur in children with asthma].

Zhong Xi Yi Jie He Xue Bao 2005 Nov;3(6):446-9

Laboratory of Syndromes of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Objective: To explore the immunological mechanism of exfoliative tongue fur in children with asthma.

Methods: Thirty-nine children with asthma, twenty-eight children with repetitive respiratory tract infection (non-asthma) and eleven healthy children were divided into five groups, which were asthma with exfoliative fur or with non-exfoliative fur groups, non-asthma with exfoliative fur or with non-exfoliative fur groups and normal control group. The concentrations of keratin 13 and bcl-2 in cells exfoliated from tongue fur were detected by immunohistochemical method. The expression levels of blood cell chemokine receptor-3 (CCR-3) and CD4(+) were examined by flow cytometry, and the levels of serum cortisol and IgE were detected by radioimmunoassay.

Results: The levels of blood CD4(+) and CCR-3 of children with asthma and exfoliative fur were higher than those in the asthma with non-exfoliative fur group and the normal control group (P<0.05). The serum level of cortisol in the groups of asthma with exfoliative fur and non-asthma with exfoliative fur were lower than that in the other groups (P<0.05). The serum levels of IgE in asthma with exfoliative fur or with non-exfoliative fur groups were higher than that in the other groups (P<0.05). Concentrations of keratin 13 in the cells exfoliated from tongue fur in the groups of asthma or non-asthma with exfoliative fur were lower than that of the other groups (P<0.05). There was no significant difference of expression level of bcl-2 in the cells exfoliated from tongue fur among these five groups.

Conclusion: There is a reasonably close relationship between the formation of exfoliative tongue fur and the immune system such as low level of serum cortisol and high levels of blood CD4(+) and CCR-3, which may all promote the formation of exfoliative fur. The disability of keratinization and apoptosis of epithelial cells of tongue may also be one cause for its formation.
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http://dx.doi.org/10.3736/jcim20050607DOI Listing
November 2005

Quality of life in patients with liver cancer after operation: a 2-year follow-up study.

Hepatobiliary Pancreat Dis Int 2004 Nov;3(4):530-3

Department of Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.

Background: Quality of life (QL) is a concept which reflects the physical, social, and emotional attitudes and behaviours of an individual. QL assessment is becoming increasingly recognised as an outcome and predictor for cancer patients. Although hepatectomy has been widely accepted as treatment of choice to offer a chance of cure for patients with liver cancer, little is known about the subjective clinical results after this operation. This prospective study was designed to evaluate the Pre- and postoperative quality of life in patients with liver cancer.

Methods: The quality of life of 36 consecutive patients was measured using gastrointestinal quality of life index (GQLI) regularly 2 years after the operation, starting with a preoperative measurement.

Results: The score of mean preoperative GQLI was 106+/-13 points, and it was reduced significantly 2-10 weeks after the operation (86-98) (P<0.05-0.001). The quality of life recovered gradually. The GQLI score was 101+/-21 points 4 months after operation and increased to the preoperative level (P>0.05). In the patients who survived more than 9 months, the GQLI score was higher than that before the operation. Major hepatectomy (lobectomy and combined segmentectomy) reduced the GQLI score more evidently than did minor hepatectomy (simple segmentectomy) in 2-5 weeks after the operation (P<0.05). The age and preoperative liver function of the patients played an important role in the recovery of the quality of life in the early postoperative stage (P<0.05). The patients with tumor recurrence showed a continuous decrease of the quality of life (P<0.05-0.001).

Conclusions: The assessment of the quality of life is meaningful for patients with liver cancer. Tumor recurrence, poor liver function and major operation are the most important factors for reducing the quality of life. Hepatic resection is justified by its effects on the survival and the quality of life of the patients.
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November 2004