Publications by authors named "Gul Erginbas-Orakci"

5 Publications

  • Page 1 of 1

Dissecting the Genetic Complexity of Fusarium Crown Rot Resistance in Wheat.

Sci Rep 2020 02 21;10(1):3200. Epub 2020 Feb 21.

Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert- Kreiten Strasse 13, D-53115, Bonn, Germany.

Fusarium crown rot (FCR) is one of the most important diseases of wheat (Triticum aestivum L.). FCR is mainly caused by the fungal pathogens Fusarium culmorum and F. pseudograminearum. In order to identify new sources of resistance to FCR and to dissect the complexity of FCR resistance, a panel of 161 wheat accessions was phenotyped under growth room (GR) and greenhouse conditions (GH). Analysis of variance showed significant differences in crown rot development among wheat accessions and high heritability of genotype-environment interactions for GR (0.96) and GH (0.91). Mixed linear model analysis revealed seven novel quantitative trait loci (QTLs) linked to F. culmorum on chromosomes 2AL, 3AS, 4BS, 5BS, 5DS, 5DL and 6DS for GR and eight QTLs on chromosomes on 3AS, 3BS, 3DL, 4BS (2), 5BS, 6BS and 6BL for GH. Total phenotypic variances (R²) explained by the QTLs linked to GR and GH were 48% and 59%, respectively. In addition, five favorable epistasis interactions among the QTLs were detected for both GR and GH with and without main effects. Epistatic interaction contributed additional variation up to 21% under GR and 7% under GH indicating strong effects of environment on the expression of QTLs. Our results revealed FCR resistance responses in wheat to be complex and controlled by multiple QTLs.
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http://dx.doi.org/10.1038/s41598-020-60190-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035263PMC
February 2020

Genome-Wide Association Study for Multiple Biotic Stress Resistance in Synthetic Hexaploid Wheat.

Int J Mol Sci 2019 Jul 26;20(15). Epub 2019 Jul 26.

Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583, USA.

Genetic resistance against biotic stress is a major goal in many wheat breeding programs. However, modern wheat cultivars have a limited genetic variation for disease and pest resistance and there is always a possibility of the evolution of new diseases and pests to overcome previously identified resistance genes. A total of 125 synthetic hexaploid wheats (SHWs; 2 = 6 = 42, AABBDD, L.) were characterized for resistance to fungal pathogens that cause wheat rusts (leaf; , stem; . f.sp. , and stripe; . f.sp. ) and crown rot (Fusarium spp.); cereal cyst nematode ( spp.); and Hessian fly (). A wide range of genetic variation was observed among SHWs for multiple (two to five) biotic stresses and 17 SHWs that were resistant to more than two stresses. The genomic regions and potential candidate genes conferring resistance to these biotic stresses were identified from a genome-wide association study (GWAS). This GWAS study identified 124 significant marker-trait associations (MTAs) for multiple biotic stresses and 33 of these were found within genes. Furthermore, 16 of the 33 MTAs present within genes had annotations suggesting their potential role in disease resistance. These results will be valuable for pyramiding novel genes/genomic regions conferring resistance to multiple biotic stresses from SHWs into elite bread wheat cultivars and providing further insights on a wide range of stress resistance in wheat.
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http://dx.doi.org/10.3390/ijms20153667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696463PMC
July 2019

Identification of Novel Quantitative Trait Loci Linked to Crown Rot Resistance in Spring Wheat.

Int J Mol Sci 2018 Sep 8;19(9). Epub 2018 Sep 8.

Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Ankara 06511, Turkey.

Crown rot (CR), caused by various species, is a major disease in many cereal-growing regions worldwide. is one of the most important species, which can cause significant yield losses in wheat. A set of 126 advanced International Maize and Wheat Improvement Center (CIMMYT) spring bread wheat lines were phenotyped against CR for field crown, greenhouse crown and stem, and growth room crown resistance scores. Of these, 107 lines were genotyped using Diversity Array Technology (DArT) markers to identify quantitative trait loci linked to CR resistance by genome-wide association study. Results of the population structure analysis grouped the accessions into three sub-groups. Genome wide linkage disequilibrium was large and declined on average within 20 cM (centi-Morgan) in the panel. General linear model (GLM), mixed linear model (MLM), and naïve models were tested for each CR score and the best model was selected based on quarantine-quarantine plots. Three marker-trait associations (MTAs) were identified linked to CR resistance; two of these on chromosome 3B were associated with field crown scores, each explaining 11.4% of the phenotypic variation and the third MTA on chromosome 2D was associated with greenhouse stem score and explained 11.6% of the phenotypic variation. Together, these newly identified loci provide opportunity for wheat breeders to exploit in enhancing CR resistance via marker-assisted selection or deployment in genomic selection in wheat breeding programs.
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http://dx.doi.org/10.3390/ijms19092666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165080PMC
September 2018

Association analysis of resistance to cereal cyst nematodes () and root lesion nematodes ( and ) in CIMMYT advanced spring wheat lines for semi-arid conditions.

Breed Sci 2016 Dec 25;66(5):692-702. Epub 2016 Oct 25.

Grains Research & Development Corporation (GRDC) , 4/4 National Circuit Barton 2600 ACT , Australia.

To identify loci linked to nematode resistance genes, a total of 126 of CIMMYT advanced spring wheat lines adapted to semi-arid conditions were screened for resistance to , , and , of which 107 lines were genotyped with 1,310 DArT. Association of DArT markers with nematode response was analyzed using the general linear model. Results showed that 11 markers were associated with resistance to (pathotype Ha21), 25 markers with resistance to , and 9 significant markers were identified to be linked with resistance to . In this work we confirmed that chromosome 4A (~90-105 cM) can be a source of resistance to as has been recently reported. Other significant markers were also identified on chromosomal regions where no resistant genes have been reported for both nematodes species. These novel QTL were mapped to chromosomes 5A, 6A, and 7A for ; on chromosomes 1A, 1B, 3A, 3B, 6B, 7AS, and 7D for ; and on chromosomes 1D, 2A, and 5B for and represent potentially new loci linked to resistance that may be useful for selecting parents and deploying resistance into elite germplasm adapted to regions where nematodes are causing problem.
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http://dx.doi.org/10.1270/jsbbs.15158DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5282747PMC
December 2016

Genome-Wide Association Study in Wheat Identifies Resistance to the Cereal Cyst Nematode Heterodera filipjevi.

Phytopathology 2016 10 23;106(10):1128-1138. Epub 2016 Aug 23.

First, fifth, sixth, and ninth authors: Institute of Crop Science and Resource Conservation (INRES), Molecular Phytomedicine, Karlrobert-Kreiten Strasse 13, D-53115 Bonn, Germany; second, fourth, and seventh authors: International Maize and Wheat Improvement Centre (CIMMYT), P.K. 39 06511, Emek, Ankara, Turkey; third and eighth authors: Institute of Crop Science and Resource Conservation (INRES), Plant Breeding, Katzenburgweg; and fifth author: Agricultural Research Center (ARC), Agricultural genetic Engineering Research Institute (AGERI), 9 Gama Street, Giza 12619, Egypt.

The cyst nematode Heterodera filipjevi is a plant parasite causing substantial yield loss in wheat. Resistant cultivars are the preferred method of controlling cyst nematodes. Association mapping is a powerful approach to detect associations between phenotypic variation and genetic polymorphisms; in this way favorable traits such as resistance to pathogens can be located. Therefore, a genome-wide association study of 161 winter wheat accessions was performed with a 90K iSelect single nucleotide polymorphism (SNP) chip. Population structure analysis grouped into two major subgroups and first principal component accounted 6.16% for phenotypic diversity. The genome-wide linkage disequilibrium across wheat was 3 cM. Eleven quantitative trait loci (QTLs) on chromosomes 1AL, 2AS, 2BL, 3AL, 3BL, 4AS, 4AL, 5BL, and 7BL were identified using a mixed linear model false discovery rate of P < 0.01 that explained 43% of total genetic variation. This is the first report of QTLs conferring resistance to H. filipjevi in wheat. Eight QTLs on chromosomes 1AL, 2AS, 2BL, 3AL, 4AL, and 5BL were linked to putative genes known to be involved in plant-pathogen interactions. Two other QTLs on 3BL and one QTL on 7BL linked to putative genes known to be involved in abiotic stress.
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http://dx.doi.org/10.1094/PHYTO-02-16-0054-FIDOI Listing
October 2016