Publications by authors named "Guimiao Jiang"

8 Publications

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Transport stress affects the fecal microbiota in healthy donkeys.

J Vet Intern Med 2021 Sep 31;35(5):2449-2457. Epub 2021 Jul 31.

National Engineering Research Center for Gelatin-based TCM, Dong-E E-Jiao Co., Ltd, Liaocheng, Shandong Province, China.

Background: With the development of large-scale donkey farming in China, long-distance transportation has become common practice, and the incidence of intestinal diseases after transportation has increased. The intestinal microbiota is important in health and disease, and whether or not transportation disturbs the intestinal microbiota in donkeys has not been investigated.

Objectives: To determine the effects of transportation on the fecal microbiota of healthy donkeys using 16S rRNA sequencing.

Animals: Fecal and blood samples were collected from 12 Dezhou donkeys before and after transportation.

Methods: Prospective controlled study. Cortisol, ACTH, and heat-shock protein 90 (HSP90) concentrations were measured. Sequencing of 16S rRNA was used to assess the microbial composition. Alpha diversity and beta diversity were assessed.

Results: Results showed significant (P < .05) increases in cortisol (58.1 ± 14.6 to 71.1 ± 9.60 ng/mL), ACTH (163.8 ± 31.9 to 315.8 ± 27.9 pg/mL), and HSP90 (10.8 ± 1.67 to 14.6 ± 1.75 ng/mL) on the day of arrival. A significantly lower (P = .04) level of bacterial richness was found in fecal samples after transportation, compared with that before transportation without distinct changes in diversity. Most notably, donkeys had significant decreases in Atopostipes, Eubacterium, Streptococcus, and Coriobacteriaceae.

Conclusions And Clinical Importance: Transportation can induce stress in healthy donkeys and have some effect on the composition of the in fecal microbiota. Additional studies are required to understand the potential effect of these microbiota changes, especially significantly decreased bacteria, on the development intestinal diseases in donkeys during recovery from transportation.
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http://dx.doi.org/10.1111/jvim.16235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478045PMC
September 2021

Cardiovascular and cerebrovascular events in patients with intracerebral hemorrhage: Clinical characteristics and long-term predictors.

J Clin Neurosci 2021 Aug 7;90:118-123. Epub 2021 Jun 7.

Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China. Electronic address:

Few studies have examined the long-term prognosis of Chinese patients with intracerebral hemorrhage (ICH). This study assessed the clinical characteristics and predictors of vascular events occurring within 5 years after ICH. We included consecutive patients diagnosed with first-ever ICH between June 2013 and December 2014. Based on follow-up data (collected until December 2019), we used multivariable logistic regression to examine the clinical characteristics and long-term predictors of vascular events (including recurrent ICH, ischemic stroke, and acute coronary syndrome) in patients who survived more than 30 days after ICH. Across the 307 patients in our analysis, the 5-year mortality rate was 28.01%. Within 5 years after ICH, major vascular events were observed in 62 patients (17.82%, 95% CI 13.78-21.82%). We observed high incidence of recurrent ICH (8.91%) and ischemic stroke (10.06%), but low incidence of acute coronary syndrome (1.15%). Most cases of recurrent ICH (80.65%) occurred within 3 years after ICH. Age ≥56 years and history of ischemic stroke or transient ischemic attack (TIA) were identified as predictors of cardiovascular and cerebrovascular events. ICH survivors are at high risk of both cardiovascular and cerebrovascular events, especially older patients (≥56 years) and those who experienced ischemic stroke or TIA prior to their first ICH. Recurrent ICH is more likely to occur within the first three years after first ICH than at later times. Clinicians should monitor patients closely for adverse events, particularly during the first three years after initial ICH.
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http://dx.doi.org/10.1016/j.jocn.2021.05.037DOI Listing
August 2021

Quantitative Label-Free Proteomic Analysis of Milk Fat Globule Membrane in Donkey and Human Milk.

Front Nutr 2021 22;8:670099. Epub 2021 Jun 22.

National Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E E-Jiao Co., Ltd, Liaocheng, China.

Previous studies have found donkey milk (DM) has the similar compositions with human milk (HM) and could be used as a potential hypoallergenic replacement diet for babies suffering from cow's milk allergy. Milk fat globule membrane (MFGM) proteins are involved in many biological functions, behaving as important indicators of the nutritional quality of milk. In this study, we used label-free proteomics to quantify the differentially expressed MFGM proteins (DEP) between DM (in 4-5 months of lactation) and HM (in 6-8 months of lactation). In total, 293 DEP were found in these two groups. Gene Ontology (GO) enrichment analysis revealed that the majority of DEP participated in regulation of immune system process, membrane invagination and lymphocyte activation. Several significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were determined for the DEP, such as lysosome, galactose metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. Our study may provide valuable information in the composition of MFGM proteins in DM and HM, and expand our knowledge of different biological functions between DM and HM.
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http://dx.doi.org/10.3389/fnut.2021.670099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258387PMC
June 2021

Tongxinluo promotes axonal plasticity and functional recovery after stroke.

Transl Neurosci 2020 25;11(1):428-438. Epub 2020 Nov 25.

Department of Neurology, the First Affiliated Hospital of Guangxi Medical University , Nanning, Guangxi Zhuang Autonomous Region, 530021, China.

Background: The aim of this study was to investigate the neural plasticity in contralesional cortex and the effects of tongxinluo (TXL) in cerebral ischemic rats.

Methodology: We used stroke-prone renovascular hypertensive (RHRSP) cerebral ischemia rat models to study the effect of TXL and the underlying mechanisms. We performed foot-fault and beam-walking tests to evaluate the motor function of rats after cortical infarction. Biotinylated dextran amine (BDA) was used to track axonal sprouting and neural connections.

Results: TXL enhanced the recovery of motor function in cerebral infarction rats. TXL increased axonal sprouting in the peri-infarcted area but not in the corpus callosum, indicating in situ origination instead of crossing between cortical hemispheres through the corpus callosum. TXL promoted the sprouting of corticospinal axons into the denervated side of spinal gray matter. The synaptophysin (SYN)-positive intensity in the peri-infarcted area of TXL-treated group was greater than that in the vehicle group. We observed co-localization of SYN with BDA-positive fibers in the denervated spinal cord gray matter in the TXL group, suggesting that axonal remodeling and synaptic connections were promoted by TXL.

Conclusion: TXL may promote the recovery of neurological function by promoting the axonal remodeling and synapse formation of motor neuronal fibers after focal cortical infarction in hypertensive rats.
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http://dx.doi.org/10.1515/tnsci-2020-0127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718613PMC
November 2020

Spontaneous Neuronal Plasticity in the Contralateral Motor Cortex and Corticospinal Tract after Focal Cortical Infarction in Hypertensive Rats.

J Stroke Cerebrovasc Dis 2020 Dec 15;29(12):105235. Epub 2020 Sep 15.

Department of Neurology, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China; Guangxi Key Laboratory of Regenerative Medicine and Guangxi Collaborative Innovation Center for Biomedicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China. Electronic address:

Objectives: In this study, we investigated the spontaneous neural plasticity on the contralateral side in hypertensive rats, including the expression of nerve growth factors (synaptophysin [SYN] and growth-associated protein 43 [GAP-43]), and the association between nerve fiber sprouting and redistribution, and the recovery of motor functions following sensorimotor cortical infarction.

Methods: Initially, Sprague-Dawley rats were induced with renal hypertension by the bilateral renal arteries clips method. Further, they were induced with cerebral ischemia by the middle cerebral artery electrocoagulation method; 70 male rats completed the study. We compared the changes in the corticospinal tract (CST) and the expressions of SYN and GAP-43 on the contralateral side in rats with cerebral infarction using immunohistochemical staining, western blot, and biotinylated dextran amine (BDA) tracing analyses. The recovery of motor function in rats after cortical infarction was evaluated by the foot-fault and beam-walk tests.

Results: The motor behavior tests revealed that the motor function of rats could recover to various degrees after focal cortical infarction. Compared with the sham-operated group, the SYN and GAP-43 levels increased in the motor cortex of the opposite hemisphere within 28 days after middle cerebral artery occlusion (MCAO). The increase in SYN and GAP-43 expressions presented differently in layers Ⅱ, Ⅲ, and Ⅴ. The amount of BDA-positive fibers also increased significantly in the denervated cervical spinal gray matter on day 56 post-MCAO.

Conclusions: The increases in SYN and GAP-43 on the contralateral side of the motor cortex could promote CST sprouting and rewiring in the spinal cord gray matter and also spontaneous motor function recovery after cortical infarction.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2020.105235DOI Listing
December 2020

Effects of long-distance transportation on blood constituents and composition of the nasal microbiota in healthy donkeys.

BMC Vet Res 2020 Sep 15;16(1):338. Epub 2020 Sep 15.

College of Veterinary Medicine, Yangzhou University, 225009, Yangzhou, P.R. China.

Background: This study aims to determine the effects of transportation on the nasal microbiota of healthy donkeys using 16S rRNA sequencing.

Results: Deep nasal swabs and blood were sampled from 14 donkeys before and after 21 hours' long-distance transportation. The values of the plasma hormone (cortisol (Cor), adrenocorticotrophic hormone (ACTH)), biochemical indicators (total protein (TP), albumin (ALB), creatinine (CREA), lactic dehydrogenase (LDH), aspartate transaminase (AST), creatine kinase (CK), blood urea (UREA), plasma glucose (GLU)) and blood routine indices (white blood cell (WBC), lymphocyte (LYM), neutrophil (NEU), red blood cell (RBC), hemoglobin (HGB)) were measured. 16S rRNA sequencing was used to assess the nasal microbiota, including alpha diversity, beta diversity, and phylogenetic structures. Results showed that levels of Cor, ACTH, and heat-shock protein 90 (HSP90) were significantly increased (p < 0.05) after long-distance transportation. Several biochemical indicators (AST, CK) and blood routine indices (Neu, RBC, and HGB) increased markedly (p < 0.05), but the LYM decreased significantly (p < 0.05). Nine families and eight genera had a mean relative abundance over 1%. The predominant phyla in nasal microbiota after and before transportation were Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Transportation stress induced significant changes in terms of nasal microbiota structure compared with those before transportation based on principal coordinate analysis (PCoA) coupled with analysis of similarities (ANOSIM) (p < 0.05). Among these changes, a notably gain in Proteobacteria and loss in Firmicutes at the phylum level was observed.

Conclusions: These results suggest transportation can cause stress to donkeys and change the richness and diversity of nasal microbiota. Further studies are required to understand the potential effect of these microbiota changes on the development of donkey respiratory diseases.
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http://dx.doi.org/10.1186/s12917-020-02563-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493398PMC
September 2020

Association between blood pressure variability and the short-term outcome in patients with acute spontaneous subarachnoid hemorrhage.

Hypertens Res 2019 11 6;42(11):1701-1707. Epub 2019 Jun 6.

Department of Neurology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

The association between the fluctuation in blood pressure (BP) and the early outcomes of patients with subarachnoid hemorrhage (SAH) remains unclear. Our study aimed to evaluate the value of blood pressure variability (BPV) for predicting the short-term outcomes of patients with acute spontaneous SAH. We collected data from 303 patients hospitalized for acute spontaneous SAH. BP values were recorded at admission and subsequently every 2 h during the initial 24 h of hospitalization. BPV was determined as the standard deviation (SD), the difference between the maximum and the minimum (ΔBP), the coefficient of variation (CV), and successive variation (SV). The outcome at discharge was assessed according to the Glasgow Outcome Scale (GOS). The association between BPV and the outcome was identified by multivariable analysis. The findings showed that the parameters of systolic BPV were independently associated with the outcome in a graded fashion. The odds ratios (OR) for the highest tertiles were as follows: SD 13.9 (95% confidence interval [CI], 4.8-40.4), ΔBP 4.4 (95% CI, 1.6-11.9), CV 16.4 (95% CI, 5.6-48.8), SV 15.8 (95% CI, 5.3-46.9). However, there was no association between a poor outcome and diastolic BPV (all p > 0.05). In conclusion, systolic BPV within the first 24 h after admission was independently associated with the outcomes in SAH patients; the greater the variability was, the worse the outcome.
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http://dx.doi.org/10.1038/s41440-019-0274-yDOI Listing
November 2019

ASIP gene variation in Chinese donkeys.

Anim Genet 2017 Jun 15;48(3):372-373. Epub 2017 Feb 15.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.

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http://dx.doi.org/10.1111/age.12530DOI Listing
June 2017
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