Publications by authors named "Guiling Zhang"

46 Publications

Clinical significance of miR-1298 in cervical cancer and its biological function .

Oncol Lett 2021 May 18;21(5):401. Epub 2021 Mar 18.

Department of Pathology, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong 250014, P.R. China.

Cervical cancer is one of the most malignant tumors in women. miR-1298 was reported to be abnormally expressed and serve crucial role in tumorigenesis of several types of cancer; however, the role of miR-1298 in cervical cancer remains unknown. The present study aimed to evaluate the clinical and biological significance of miR-1298 in cervical cancer. To do so, the expression level of miR-1298 in cervical cancer tissues and cells was evaluated by reverse transcription quantitative PCR. Kaplan-Meier survival analysis and Cox regression analysis were used to explore the prognostic significance of miR-1298 in patients with cervical cancer. Cell Counting Kit-8 and Transwell migration and invasion assays were used to evaluate the effect of miR-1298 on the proliferative, migratory and invasive abilities of cervical cancer cells, respectively. The expression of miR-1298 was lower in cancer tissues and cells compared with normal tissues and cells. Furthermore, miR-1298 expression was associated with lymph node metastasis, tumor diameter and staging from the International Federation of Gynecology and Obstetrics. In addition, patients with low miR-1298 expression had poorer overall survival. These findings suggested that miR-1298 may be considered as an independent prognostic factor for patients with cervical cancer. Furthermore, the results demonstrated that miR-1298 knockdown could promote tumor cell proliferation and migratory and invasive abilities. In addition, nucleus accumbens-associated 1 (NACC1) was demonstrated to be a direct target of miR-1298. Taken together, these findings indicated that miR-1298 overexpression may be considered as a prognostic biomarker for cervical cancer and that miR-1298 may play an inhibitor role in cervical cancer by targeting NACC1.
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http://dx.doi.org/10.3892/ol.2021.12662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988695PMC
May 2021

Electronic Properties of Triangle Molybdenum Disulfide (MoS) Clusters with Different Sizes and Edges.

Molecules 2021 Feb 22;26(4). Epub 2021 Feb 22.

School of Physics, Harbin Institute of Technology, Harbin 150001, China.

The electronic structures and transition properties of three types of triangle MoS clusters, (Mo edge passivated with two S atoms), (Mo edge passivated with one S atom), and (S edge) have been explored using quantum chemistry methods. The highest occupied molecular orbital (HOMO)-lowest unoccupied molecular orbital (LUMO) gap of B and C is larger than that of A, due to the absence of the dangling of edge S atoms. The frontier orbitals (FMOs) of A can be divided into two categories, edge states from S at the edge and hybrid states of Mo and S covering the whole cluster. Due to edge/corner states appearing in the FMOs of triangle MoS clusters, their absorption spectra show unique characteristics along with the edge structure and size.
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http://dx.doi.org/10.3390/molecules26041157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927058PMC
February 2021

The complete chloroplast genome sequence of herb (family: Valerianaceae) in China.

Mitochondrial DNA B Resour 2021 Jan 27;6(1):254-256. Epub 2021 Jan 27.

School of Chemistry and Materials Science, Guizhou Education University, Guiyang, China.

is an endemic herb in China, distributes mainly in Southeast Gansu, South Qinghai and West Sichuan of Qinghai-Tibet Plateau. In this study, the complete chloroplast genome (a typical quadripartite structure) sequence of was reported. The length of the DNA molecule was 155,268 bp with a large single-copy region (LSC: 87,263 bp), small single-copy region (SSC: 17,327 bp) and inverted repeats (IRa and IRb: 25,339 bp). The overall GC content was 38.56%. It has a total of 129 genes, containing 83 protein-coding genes, 38 tRNA genes, and eight rRNA genes. The phylogenetic analysis has shown that is sister to The chloroplast genome provides the basis for development and utilization of in future.
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http://dx.doi.org/10.1080/23802359.2020.1861568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872520PMC
January 2021

Long-term microstructure and cerebral blood flow changes in patients recovered from COVID-19 without neurological manifestations.

J Clin Invest 2021 04;131(8)

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

BACKGROUNDThe coronavirus disease 2019 (COVID-19) rapidly progressed to a global pandemic. Although some patients totally recover from COVID-19 pneumonia, the disease's long-term effects on the brain still need to be explored.METHODSWe recruited 51 patients with 2 subtypes of COVID-19 (19 mild and 32 severe) with no specific neurological manifestations at the acute stage and no obvious lesions on the conventional MRI 3 months after discharge. Changes in gray matter morphometry, cerebral blood flow (CBF), and white matter (WM) microstructure were investigated using MRI. The relationship between brain imaging measurements and inflammation markers was further analyzed.RESULTSCompared with healthy controls, the decrease in cortical thickness/CBF and the changes in WM microstructure were more severe in patients with severe disease than in those with mild disease, especially in the frontal and limbic systems. Furthermore, changes in brain microstructure, CBF, and tract parameters were significantly correlated (P < 0.05) with the inflammatory markers C-reactive protein, procalcitonin, and interleukin 6.CONCLUSIONIndirect injury related to inflammatory storm may damage the brain, altering cerebral volume, CBF, and WM tracts. COVID-19-related hypoxemia and dysfunction of vascular endothelium may also contribute to neurological changes. The abnormalities in these brain areas need to be monitored during recovery, which could help clinicians understand the potential neurological sequelae of COVID-19.FUNDINGNatural Science Foundation of China.
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http://dx.doi.org/10.1172/JCI147329DOI Listing
April 2021

Effects of childhood trauma experience and BDNF Val66Met polymorphism on brain plasticity relate to emotion regulation.

Behav Brain Res 2021 02 11;398:112949. Epub 2020 Oct 11.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic address:

Brain-derived neurotrophic factor (BDNF) Val66Met polymorphism may modulate the link between childhood trauma experience and psychopathology by altering trophic signaling on neuroplasticity. However, few multimodal magnetic resonance imaging (MRI) researches have investigated this gene-environment interaction on both structural and functional plasticity, thereby advancing knowledge about the etiology, prevention, and customized therapeutic directions of mental disease in individuals with childhood trauma experience. We recruited a large non-clinical sample of young adults that completed Childhood Trauma Questionnaire (CTQ), behavioral scores, multimodal MRI scans, and genotyping. Morphometric similarity network (MSN) and independent component analysis were adopted to quantify brain structural and functional changes. Gene-environment-brain-behavior relationships were identified by multiple regression and mediation effect analysis. CTQ score was positively associated with depression and anxiety scores. We found interactions on MSN in sensorimotor, temporal, and orbitofrontal cortex. For intra-network connectivity, significant interaction was noted in clusters within sensorimotor network. For inter-network connectivity, connectivity between dorsal attention network and salience network showed an interactive effect. For mean connectivity strength of each network, we found a main effect of CTQ score on self-reference network that was an outstanding mediator supporting the relationship between CTQ score and anxiety. Our findings demonstrate that childhood trauma and the BDNF Val66Met polymorphism are associated with brain plasticity involving emotion regulation, structurally and functionally, which may contribute to understanding psychotic mechanisms and predicting differential susceptibility. Imaging genetics may be useful as biomarkers to provide early assessment and guide cognitive interventions to avoid or decrease the risk of developing psychopathology.
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http://dx.doi.org/10.1016/j.bbr.2020.112949DOI Listing
February 2021

Effects of childhood trauma experience and COMT Val158Met polymorphism on brain connectivity in a multimodal MRI study.

Brain Behav 2020 12 30;10(12):e01858. Epub 2020 Sep 30.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Childhood adversity may act as a stressor to produce a cascade of neurobiological effects that irreversibly alter neural development, setting the stage for developing psychopathology in adulthood. The catechol-O-methyltransferase (COMT) Val158Met polymorphism has received much attention as a candidate gene associated with environmental adversity, modifying risk for psychopathology. In this study, we aim to see how gene × brain × environment models give a more integrative understanding of brain modifications that contribute to predicting psychopathology related to childhood adversity. A large nonclinical sample of young adults completed Childhood Trauma Questionnaire (CTQ), behavioral scores, multimodal magnetic resonance imaging (MRI) scans, and genotyping. We utilized graph-based connectivity analysis in morphometric similarity mapping and resting-state functional MRI to investigate brain alterations. Relationships among COMT genotypes, CTQ score, imaging phenotypes, and behavioral scores were identified by multiple regression and mediation effect analysis. Significant main effect of CTQ score was found in anatomic connectivity of orbitofrontal cortex that was an outstanding mediator supporting the relationship between CTQ score and anxiety/harm-avoiding personality. We also noted the main effect of childhood trauma on reorganization of functional connectivity within the language network. Additionally, we found genotype × CTQ score interactions on functional connectivity of the right frontoparietal network as well as anatomic connectivity of motor and limbic regions. Our data demonstrate childhood adversity and COMT genotypes are associated with abnormal brain connectivity, structurally and functionally. Early identification of individuals at risk, assessment of brain abnormality, and cognitive interventions may help to prevent or limit negative outcomes.
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http://dx.doi.org/10.1002/brb3.1858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749512PMC
December 2020

Correlations between hormone levels and cardiovascular autonomic neuropathy in menopausal patients with diabetes mellitus.

Pak J Med Sci 2020 Sep-Oct;36(6):1382-1386

Jingqin Liu, Department of Endocrinology, Baoding No.1 Hospital, Baoding, Hebei 071000, P.R. China.

Objective: To discuss the correlation between hormone levels and cardiovascular autonomic neuropathy (CAN) in menopausal patients with Type-2 diabetes mellitus (T2DM).

Methods: This clinical research study was conducted at Department of Endocrinology, Baoding No. 1 Hospital from January 2016 to December 2017. In this study a total of 386 menopausal female patients with T2 DM were selected and classified into two groups according to the CAN function test: the CAN group (80 cases) and the DM group (306 cases). The Kupperman score (KI integral) was calculated for all participants in the study, and the following indexes were measured: body mass index (BMI), blood estrogen (E), follicle-stimulating hormone (FSH), luteinizing hormone (LH), thyroid-stimulating hormone (TSH), free thyroxine (FT), free triiodothyronine (FT), fasting blood-glucose (FBG), glycosylated hemoglobin (HbA1c), serum lipids, uric acid (SUA), hypersensitive c-reactive protein (CRP), etc.

Results: The FBG, HbA1c, TGs, Hs-CRP, SUA, KI score, TSH, FSH and LH of the CAN group were obviously higher than the same parameters in the DM group (P<0.01, P<0.05), while HDL-C, E, FT and FT were significantly lower (Pπ.01, Pπ.05). Pearson correlation analysis indicated that CAN presents a positive correlation with HbA1c, TGs, hs-CRP and SUA and a negative correlation with HDL-C and E, and the difference was statistically significant (P<0.05). The multifactor logistic regression analysis results showed that HbA1c (OR=3.980, 95%CI=1.268~10.319) and E2 (OR=3.075, 95%CI=1.167~7.366) are independent risk factors for CAN.

Conclusion: The CAN morbidity of menopausal female patients with T2DM is high, and HbAc and E should be mainly monitored to identify and treat CAN early.
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http://dx.doi.org/10.12669/pjms.36.6.2088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501038PMC
September 2020

Modulation of the electronic band structure of silicene by polar two-dimensional substrates.

Phys Chem Chem Phys 2020 Sep;22(37):21412-21420

School of Physics, Harbin Institute of Technology, Harbin 150001, China.

Using the density functional theory (DFT) calculations, we find that  Janus group-III chalcogenide monolayers can serve as a suitable substrate for silicene, and the Dirac electron band properties of silicene are also fully preserved. The maximum opened band gap can reach 179 meV at the Dirac point due to the interaction of silicene and the polar two-dimensional (2D) substrate. In addition, the electronic band structure of the heterostructure can be modulated by applying an electric field where its predicted band gap increases or decreases according to the direction of the applied external electric field. Furthermore, an insight into the electron structures can be understood by analyzing the electron energy-loss (EEL) spectra. From these results, we also predict that heterostructures with polar 2D substrates have broad application prospects in multi-functional devices. Besides, Janus group-III chalcogenide monolayers can be used as good substrates for growing silicene and the modulation of the electronic structure can also be applied to nanodevices and optoelectronic devices.
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http://dx.doi.org/10.1039/d0cp03486jDOI Listing
September 2020

Novel proton exchange rate MRI presents unique contrast in brains of ischemic stroke patients.

J Neurosci Methods 2020 12 5;346:108926. Epub 2020 Sep 5.

Departments of Radiology, Department of Bioengineering, the Center for MR Research, University of Illinois at Chicago, Chicago, USA. Electronic address:

Background: To map and quantify the proton exchange rate (k) of brain tissues using improved omega plots in ischemic stroke patients and to investigate whether k can serve as a potential endogenous surrogate imaging biomarker for detecting the metabolic state and the pathologic changes due to ischemic stroke.

New Method: Three sets of Z-spectra were acquired from seventeen ischemic stroke patients using a spin echo-echo planar imaging sequence with pre-saturation chemical exchange saturation transfer (CEST) pulse at B of 1.5, 2.5, and 3.5 μT, respectively. Pixel-wise k was calculated from improved omega plot of water direct saturation (DS)-removed Z-spectral signals.

Results: The derived k maps can differentiate infarcts from contralateral normal brain tissues with significantly increased signal (893 ± 52 svs. 739 ± 34 s, P < 0.001).

Comparison With Existing Method(s): The k maps were found to be different from conventional contrasts from diffusion-weighted imaging (DWI), CEST, and semi-solid magnetization transfer (MT) MRI. In brief, k MRI showed larger lesion areas than DWI with different degrees and different lesion contrast compared to CEST and MT.

Conclusions: In this preliminary translational research, the k MRI based on DS-removed omega plots has been demonstrated for in vivo imaging of clinical ischemic stroke patients. As a noninvasive and unique MRI contrast, k MRI at 3 T may serve as a potential surrogate imaging biomarker for the metabolic changes of stroke and help for monitoring the evolution and the treatment of stroke.
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http://dx.doi.org/10.1016/j.jneumeth.2020.108926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606776PMC
December 2020

Cryptosporidium parvum upregulates miR-942-5p expression in HCT-8 cells via TLR2/TLR4-NF-κB signaling.

Parasit Vectors 2020 Aug 31;13(1):435. Epub 2020 Aug 31.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, P. R. China.

Background: Micro (mi)RNAs are small noncoding RNA molecules that function in RNA silencing and post-transcriptional regulation of gene expression. This study investigated host miRNA activity in the innate immune response to Cryptosporidium parvum infection.

Methods: In vitro infection model adopts HCT-8 human ileocecal adenocarcinoma cells infected with C. parvum. The expression of miR-942-5p was estimated using quantitative real-time polymerase chain reaction (qPCR). The TLRs-NF-κB signaling was confirmed by qPCR, western blotting, TLR4- and TLR2-specific short-interfering (si)RNA, and NF-κB inhibition.

Results: HCT-8 cells express all known toll-like receptors (TLRs). Cryptosporidium parvum infection of cultured HCT-8 cells upregulated TLR2 and TLR4, and downstream TLR effectors, including NF-κB and suppressed IκBα (nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha). The expression of miR-942-5p was significantly upregulated at 4, 8, 12 and 24 h post-infection, and especially at 8 hpi. The results of TLR4- and TLR2-specific siRNA and NF-κB inhibition showed that upregulation of miR-942-5p was promoted by p65 subunit-dependent TLR2/TLR4-NF-κB pathway signaling.

Conclusions: miR-942-5p of HCT-8 cells was significantly upregulated after C. parvum infection, especially at 8 hpi, in response to a p65-dependent TLR2/TLR4-NF-κB signaling. TLR4 appeared to play a dominant role.
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http://dx.doi.org/10.1186/s13071-020-04312-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461316PMC
August 2020

Ultrathin Phosphate-Modulated Co Phthalocyanine/g-CN Heterojunction Photocatalysts with Single Co-N (II) Sites for Efficient O Activation.

Adv Sci (Weinh) 2020 Aug 25;7(16):2001543. Epub 2020 Jun 25.

Key Laboratory of Functional Inorganic Materials Chemistry School of Chemistry and Material Sciences Heilongjiang University Harbin Heilongjiang 150080 China.

Realization of solar-driven aerobic organic transformation under atmospheric pressure raises the great challenge for efficiently activating O by tailored photocatalysts. Guided by theoretical calculation, phosphate groups are used to induce the construction of ultrathin Co phthalocyanine/g-CN heterojunctions (CoPc/P-CN, ≈4 nm) via strengthened H-bonding interfacial connection, achieving an unprecedented 14-time photoactivity improvement for UV-vis aerobic 2,4-dichlorophenol degradation compared to bulk CN by promoted activation of O. It is validated that more O radicals are produced through the improved photoreduction of O by accelerated photoelectron transfer from CN to the ligand of CoPc and then to the abundant single Co-N (II) catalytic sites, as endowed by the matched dimension, intimate interface even at the molecular level, and high CoPc dispersion of resulted heterojunctions. Interestingly, CoPc/P-CN also exhibits outstanding photoactivities in the aerobic oxidation of aromatic alcohols. This work showcases a feasible route to realize efficient photocatalytic O activation by exploiting the potential of ultrathin metal phthalocyanine (MPc) assemblies with abundant single-atom sites. More importantly, a universal facile strategy of H-bonding-dominating construction of MPc-involved heterojunctions is successfully established.
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http://dx.doi.org/10.1002/advs.202001543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435235PMC
August 2020

Enhanced Shift Currents in Monolayer 2D GeS and SnS by Strain-Induced Band Gap Engineering.

ACS Omega 2020 Jul 9;5(28):17207-17214. Epub 2020 Jul 9.

Chongqing Key Laboratory of Theoretical and Computational Chemistry, College of Chemistry and Chemical Engineering, Chongqing University, Huxi Campus, Chongqing 401331, China.

Group IV monochalcogenides exhibit spontaneous polarization and ferroelectricity, which are important in photovoltaic materials. Since strain engineering plays an important role in ferroelectricity, in the present work, the effect of equibiaxial strain on the band structure and shift currents in monolayer two-dimensional (2D) GeS and SnS has systematically been investigated using the first-principles calculations. The conduction bands of those materials are more responsive to strain than the valence bands. Increased equibiaxial compressive strain leads to a drastic reduction in the band gap and finally the occurrence of phase transition from semiconductor to metal at strains of -15 and -14% for GeS and SnS, respectively. On the other hand, tensile equibiaxial strain increases the band gap slightly. Similarly, increased equibiaxial compressive strain leads to a steady almost four times increase in the shift currents at a strain of -12% with direction change occurring at -8% strain. However, at phase transition from semiconductor to metal, the shift currents of the two materials completely vanish. Equibiaxial tensile strain also leads to increased shift currents. For SnS, shift currents do not change direction, just as the case of GeS at low strain; however, at a strain of +8% and beyond, direction reversal of shift currents beyond the band gap in GeS occur.
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http://dx.doi.org/10.1021/acsomega.0c01319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376894PMC
July 2020

Theoretical study of CO hydrogenation on Cu surfaces.

J Mol Model 2020 Jul 9;26(8):202. Epub 2020 Jul 9.

Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China.

CO reduction has attracted extensive attentions for its wide applications in chemical engineering and green chemistry. As one of major commercial catalysts, Cu have been widely studied considering its low price and high catalytic efficiency. However, previous studies were mostly focused on the Cu(111) surface, while other surfaces were rarely studied. In this work, we employed the density functional theory calculations to fully investigate the adsorption of all intermediates and products of CO hydrogenation on three low-index surfaces as Cu(111), Cu(100), and Cu(110), which have been reported as the main facets of Cu nanoparticles under reaction conditions. Besides, the reaction pathways were also discussed. Our results indicated CO hydrogenation is preferred to adopt formate pathways on the Cu surfaces, while the COOH pathway is least favorable. Moreover, Cu(100) and Cu(110) surfaces have the comparable (even better) catalytic activities compared with Cu(111) surface. This study provides the fundamental data for the adsorption and reaction of CO hydrogenation, which will be helpful for the design of Cu-based nanocatalysts.
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http://dx.doi.org/10.1007/s00894-020-04448-8DOI Listing
July 2020

Age and anatomical location related hemodynamic changes assessed by 4D flow MRI in the carotid arteries of healthy adults.

Eur J Radiol 2020 Jul 4;128:109035. Epub 2020 May 4.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Purpose: To evaluate hemodynamic changes (volume, velocity, wall shear stress, pressure gradient, and energy loss) in the common carotid artery (CCA) and internal carotid artery (ICA) of healthy adults among different ages and anatomical locations using 4D flow MRI.

Methods: Sixty-two healthy volunteers aged 20-75 years were enrolled in this study. 4D flow MRI examinations were performed for each subject and were analyzed using the CVI42 platform to generate hemodynamic parameters. Hemodynamic parameters were compared among different age groups and anatomical locations (proximal CCA, distal CCA, proximal ICA and distal CCA) using one-way ANOVA. The paired t-test was used to estimate the differences between left and right vessels. The relationship between age and hemodynamic parameters was quantified by Pearson's correlation coefficient.

Results: There were no differences between the left and right carotid arteries for any of the hemodynamic parameters (all p values > 0.05), so we set each vessel as an independent sample. The proximal ICA had significantly lower volume, velocity, wall shear stress, and pressure gradient values than the values determined for other locations (p < 0.05), and energy loss was similar among different locations. Wall shear stress (except in the distal ICA), velocity, pressure gradient, and energy loss decreased with age (p < 0.05).

Conclusions: The multiparameter analysis of 4D flow MRI can identify age and anatomical location changes in hemodynamic parameters in the carotid arteries of healthy adults. The lower velocity, wall shear stress, and pressure gradient in the proximal ICA and the reduced trend with age may be associated with disease occurrence.
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http://dx.doi.org/10.1016/j.ejrad.2020.109035DOI Listing
July 2020

[Detection of a BRCA1 c.2013_2014ins GT variant an ethnic Han Chinese pedigree affected with breast cancer].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Apr;37(4):415-418

Department of Head, Neck and Breast Surgery, Xinxiang Central Hospital, Henan 453000, China.

Objective: To detect potential variant in an ethical Han Chinese pedigree affected with breast cancer.

Methods: The proband and her relatives were subjected to next-generation sequencing using a target capture sequencing kit containing 121 cancer-related genes. Candidate variants were selected by analysis of their type, frequency in population, and segregation with the phenotype. Candidate variant was verified by Sanger sequencing and TA cloning.

Results: A c.2013_2014ins GT variant was detected in the BRCA1 gene among all breast cancer patients from this pedigree but not among healthy females. The variant was not recorded in the 1000 Genome Project database or the Exome Aggregation Consortium (ExAC) database. The frameshifting insertion was predicted to form an premature stop codon in gene transcript and can give rise to a truncated protein.

Conclusion: The BRCA1 c.2013_2014ins GT variant probably underlies the pathogenesis of breast cancer in this Chinese pedigree.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2020.04.012DOI Listing
April 2020

Multiple factors dominate the distribution of methane and its sea-to-air flux in the Bohai Sea in summer and autumn of 2014.

Mar Pollut Bull 2020 May 7;154:111049. Epub 2020 Mar 7.

State Key Laboratory of Severe Weather & Key Laboratory of Atmospheric Chemistry of CMA, Chinese Academy of Meteorological Sciences, South Zhongguancun Street, Beijing 100081, China. Electronic address:

The Bohai Sea is well-known as a source of atmospheric methane (CH). However, the main regulate factors of the spatiotemporal distribution of CH and its sea-to-air flux remain largely unknown. In this study, the observed CH concentration ranged from 4.8 to 32.7 nmol/L and 3.1 to 15.2 nmol/L in August and November of 2014, respectively. The main factors that influence the distribution of CH and its sea-to-air flux were stratification, solubility, and current structure for the mid-west depression basins, the permanent well-mixed seawater column and CH source strength for the centre shallow ridge zone, and the upwelling for the east depression basin, respectively. Meanwhile, wind also plays an important role in sea-to-air CH flux in the study area except the centre shallow ridge zone. Upwelling made the east depression basin the most intensive source of CH, with a flux of 2 to 4 times higher than the other sub-regions.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111049DOI Listing
May 2020

Lengthening the aptamer to hybridize with a stem-loop DNA assistant probe for the electrochemical detection of kanamycin with improved sensitivity.

Anal Bioanal Chem 2020 Apr 19;412(11):2391-2397. Epub 2020 Feb 19.

School of Materials Science and Engineering, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150040, Heilongjiang, China.

By adding 6 thymines to lengthen the parent aptamer combined with the change of "on" and "off" induced by the target for an assistant stem-loop DNA probe (ASP-SLP-MB), a new folding-type electrochemical kanamycin (Kana) aptamer-engineering dual-probe-based sensor (sensor d) was developed. By purposefully reducing the background current and increasing the electron transfer efficiency of methylene blue (MB), the sensor obtained significantly enhanced detection sensitivity compared with non-aptamer-engineering one-probe-based sensor (sensor a). Such efficacy was validated by a big decrease from 530.6 to 210.2 nA for the background current signal and from 360 to 0.3 nM for the detection limit. In addition to the improved sensitivity, the sensor also exhibited good selectivity, anti-fouling detection performance, and potential quantitative analysis ability, showing a feasible potential practical analytical application in real-life complicated samples, for example, milk and serum. The released results prove that the aptamer-engineering method is effective in improving the analytical performance of folding-type sensors and provides a methodological guidance for the design and fabrication of other high-performance folding-type aptasensors. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-020-02481-3DOI Listing
April 2020

Perfect Spin Filtering in Homobimetallic Ni Complex with High Tolerance to Structural Changes.

J Phys Chem Lett 2019 Dec 6;10(24):7842-7849. Epub 2019 Dec 6.

School of Physics , Harbin Institute of Technology , Harbin 150001 , China.

In the theory of ligand fields, depending on the nature and field strength of the surrounding ligands, the central metal ion may exhibit different electronic configurations, low spin (LS) or high spin (HS). Realizing stable spin polarization is one of the main challenges in the field of molecular spintronic devices because of spin switching triggered by an external stimulus. Here, an asymmetric homobimetallic complex has been investigated using the nonequilibrium Green's function and spin density functional theory. Our calculations indicate that the homobimetallic complex can achieve negative differential resistance, rectification effect, and perfect spin filtering transport on the level of an individual molecule. Strikingly, when the molecule is stretched by 0.45 Å, the HS state is still the most stable because of the weak magnetic Ni-Ni interaction. Although its conductivity decreases by 30%, the efficiency of spin filtering remains 100%. These obtained theoretical findings suggest that the homobimetallic complexes hold great potential in molecular spintronics.
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http://dx.doi.org/10.1021/acs.jpclett.9b02954DOI Listing
December 2019

Mixed-Metal-Cluster Strategy for Boosting Electrocatalytic Oxygen Evolution Reaction of Robust Metal-Organic Frameworks.

ACS Appl Mater Interfaces 2019 Dec 21;11(48):45080-45086. Epub 2019 Nov 21.

School of Materials Science and Engineering, College of Chemical and Environmental Engineering , Harbin University of Science and Technology , Harbin 150040 , China.

Rational construction of mixed metal-organic frameworks (MOFs) has been proved to be an effective way to heighten the electrocatalytic performance for MOFs, while the function of mixed metal clusters in MOFs to the electrocatalytic activity has not been known. For the first time, we present a mixed-metal-cluster strategy to boost electrocatalytic oxygen evolution reaction (OER) performance for MOFs. Heterometal clusters (FeM(μ-O) (CHCOO)(HO), denoted as FeM (M: Co or Ni)), were chosen as the metal source to construct two bimetal PCN-250-FeM. Then, we further mixed FeCo and FeNi clusters to construct mixed-metal-cluster PCN-FeCo-FeNi by the in situ solvothermal reaction. Consequently, the OER activity of PCN-FeCo-FeNi shows a dramatic enhancement compared with that of the parent bimetal MOFs. The PCN-FeCo-FeNi displays a lower overpotential of 271 mV (η), small Tafel slope (67.7 mV dec), and good linear sweep voltammetry cycle stability for the OER. Combination of DFT calculation and experiment results show that the improved electrocatalytic activity of PCN-FeCo-FeNi is ascribed to the increased electron density of Co and Ni active centers and electrochemically active areas.
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http://dx.doi.org/10.1021/acsami.9b14995DOI Listing
December 2019

Transport and Photoelectric Properties of 2D Silicene/MX (M = Mo, W; X = S, Se) Heterostructures.

ACS Omega 2018 Oct 16;3(10):13251-13262. Epub 2018 Oct 16.

Department of Physics, Harbin Institute of Technology, Harbin 150001, China.

The transport and photoelectric properties of four two-dimensional (2D) silicene/MX (M = Mo, W; X = S, Se) heterostructures have been investigated by employing density functional theory, nonequilibrium Green's function, and Keldysh nonequilibrium Green's function methods. The stabilities of silicene (SiE) are obviously improved after being placed on the MX (M = Mo, W; X = S, Se) substrates. In particular, the conductivities of SiE/MX are enhanced compared with free-standing SiE and MX. Moreover, the conductivities are increased with the group number of X, i.e., in the order of SiE < SiE/MS < SiE/MSe. An evident current oscillation phenomenon is observed in the SiE/WX heterostructures. When a linear light illumination is applied, SiE/MSe shows a stronger photoresponse than SiE/MS. The maximum photoresponse with a value of 9.0/photon was obtained for SiE/WSe. More importantly, SiE/MS (M = Mo, W) heterostructures are good candidates for application in designing solar cells owing to the well spatial separation of the charge carriers. This work provides some clues for further exploring 2D SiE/MX heterostructures involving tailored photoelectric properties.
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http://dx.doi.org/10.1021/acsomega.8b01282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6644475PMC
October 2018

Changes of white matter integrity and structural network connectivity in nondemented cerebral small-vessel disease.

J Magn Reson Imaging 2020 04 26;51(4):1162-1169. Epub 2019 Aug 26.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Previous studies have found widespread impairment of white matter (WM) integrity and disruption of structural network connectivity in cerebral small-vessel disease, but have not evaluated these changes jointly in nondemented patients.

Purpose: To jointly investigate the microstructural impairment of WM and the related alterations of structural network topology in nondemented cerebral small-vessel disease (CSVD-ND).

Study Type: Prospective.

Population: Thirty-seven CSVD-ND patients and 34 elderly controls, who were age-, sex-, and education-matched.

Field Strength/sequence: 3.0T/diffusion tensor imaging (DTI).

Assessment: Clinical characteristics, lacunar infarct, and white matter hyperintensity (WMH) was assessed. A multiatlas likelihood fusion (MALF) algorithm was used for DTI-based brain segmentation and network node defining. Then the alterations of WM integrity and structural network topology were investigated jointly.

Statistical Tests: Student's t-test, chi-square test, Mann-Whitney U-test, linear regression, Pearson correlation, and multiple comparison correction.

Results: Decreased fractional anisotropy and increased trace values were observed in predefined structures (P < 0.05, familywise error rate-corrected), including major commissural fibers, projection fibers, and some association fibers. Topologically, both groups showed small-worldness. CSVD-ND patients showed reduced global and local efficiency (P < 0.001). Despite widespread impairment of WM integrity, CSVD-ND patients only showed reduced nodal efficiency in the right superior occipital gyrus and the right lingual gyrus (P < 0.05, familywise error rate-corrected). The nodal local efficiency of the right precuneus was associated with the processing speed after adjusting the effect of lacunar infarct and WMH (r = -0.499, P = 0.038).

Data Conclusion: WM integrity was widely impaired in nondemented CSVD patients but structural network connectivity was relatively preserved. DTI may potentially provide information for the pathophysiology of CSVD in the nondemented phase.

Level Of Evidence: 1 Technical Efficacy: Stage 3 J. Magn. Reson. Imaging 2020;51:1162-1169.
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http://dx.doi.org/10.1002/jmri.26906DOI Listing
April 2020

Two kanamycin electrochemical aptamer-based sensors using different signal transduction mechanisms: A comparison of electrochemical behavior and sensing performance.

Bioelectrochemistry 2019 Oct 19;129:270-277. Epub 2019 Jun 19.

School of Materials Science and Engineering, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China. Electronic address:

Two typical kanamycin-A (KAN-A) electrochemical aptamer-based sensors employing different signal transduction mechanisms were deliberately designed and constructed with a similar structure. One sensor (sensor-1) was based on the classical probe conformation changing mode (PCCM) with a methylene blue (MB) label used as an electrochemical tag; the other sensor (sensor-2) used the target-induced signal probe shifting (TISPS) method with a free MB label in the assay solution. The difference in signal transduction mechanisms resulted in big differences in basic electrochemical behavior and comprehensive sensing performance. The results show that both sensor types exhibit different electrochemical behavior in square wave voltammetry, cyclic voltammetry, and in sensitivity, with detection limits of 3.0 nM for sensor-1 and 60.0 pM for sensor-2 in buffer. When validated for practical and quantitative detection of tap water and milk samples, both sensing methods performed well with detection limits of <260 nM and measurement times of <40 min. In addition, accuracy was good with mean recoveries of 72.3-92.6% and precision was acceptable with a relative standard deviation (RSD) of ≤14.2%. The basis for the similarities and differences in performance is also presented.
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http://dx.doi.org/10.1016/j.bioelechem.2019.06.004DOI Listing
October 2019

Chiral heteronanotubes: arrangement-dominated chiral interface states and conductivities.

Nanoscale 2019 May;11(18):8699-8705

Department of Physics, Harbin Institute of Technology, Harbin, 150001, P. R. China.

Structural analogue between pure carbonic nanostructures and their boron nitride counterparts provides possibilities for the fabrication of BCN hetero-nanomaterials, which have attracted widespread interest and been synthesized with stacked-layer, monolayer and tubular morphologies. In this work, the arrangement-dominated chiral interface states and conductivities of BCN heteronanotubes are investigated in detail by first principles calculations. The π-conjugated states can be driven by the high potential barrier of insulating BN domains to form chiral transport states along the interfaces. The emerging antiparallel and parallel chiral interface states play a dominant role for resonant transport and provide possibilities for the formation of chiral currents. Moreover, the unidirectional chiral currents have advantages to induce a magnetic field which can reach over 0.1 T. In contrast to the parallel-arranged chiral heteronanotubes, the antiparallel-arranged chiral heteronanotubes with the same stoichiometry have narrower band-gaps and stronger chiral conductivities. Such arrangement-dominated chiral transport interface states endow CHNTs with potential application in magneto-electronics.
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http://dx.doi.org/10.1039/c9nr01996kDOI Listing
May 2019

Amid proton transfer (APT) and magnetization transfer (MT) MRI contrasts provide complimentary assessment of brain tumors similarly to proton magnetic resonance spectroscopy imaging (MRSI).

Eur Radiol 2019 Mar 13;29(3):1203-1210. Epub 2018 Aug 13.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 JieFang Avenue, Hankou, Wuhan, 430030, People's Republic of China.

Objectives: Using MRSI as comparison, we aimed to explore the difference between amide proton transfer (APT) MRI and conventional semi-solid magnetization transfer ratio (MTR) MRI, and to investigate if molecular APT and structural MTR can provide complimentary information in assessing brain tumors.

Methods: Seventeen brain tumor patients and 17 age- and gender-matched volunteers were included and scanned with anatomical MRI, APT and MT-weighted MRI, and MRSI. Multi-voxel choline (Cho) and N-acetylaspartic acid (NAA) signals were quantified from MRSI and compared with MTR and MTR contrasts averaged from corresponding voxels. Correlations between contrasts were explored voxel-by-voxel by pooling values from all voxels into Pearson's correlation analysis. Differences in correlation coefficients were tested with the Z-test (set at p<0.05).

Results: APT and MT provide good contrast and quantitative parameters in tumor imaging, as do the metabolite (Cho and NAA) maps. MTR significantly correlated with MTR (R=-0.61, p<0.0001), Cho (R=0.568, p<0.0001) and NAA (R=-0.619, p<0.0001) in tumors, and MTR also significantly correlated with Cho (R=-0.346, p<0.0001) and NAA (R=0.624, p<0.0001). In healthy volunteers, MTR was non-significantly correlated with MTR (R=-0.049, p=0.239), Cho (R=0.030, p=0.478) and NAA (R=-0.083, p=0.046). Significant correlations were found among MTR with Cho (R=0.199, p<0.0001) and NAA (R=0.263, p<0.0001) in the group of healthy volunteers with lower correlation R values than those in tumor patients.

Conclusions: APT and MT could provide independent and supplementary information for the comprehensive assessment of molecular and structural changes due to brain tumor cancerogenesis.

Key Points: • MTR positively correlated with Cho while negatively with NAA in tumors. • MTR positively correlated with NAA while negatively with Cho in tumors. • Combining APT/MT provides molecular and structural information similarly to MRSI.
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http://dx.doi.org/10.1007/s00330-018-5615-8DOI Listing
March 2019

A computational study on a multimode spin conductance switching by coordination isomerization in organometallic single-molecule junctions.

Phys Chem Chem Phys 2018 Aug;20(30):20280-20286

School of Materials Science and Engineering, Harbin University of Science and Technology, Harbin 150080, China.

Single-molecule junctions provide the additional flexibility of tuning the on/off conductance states through molecular design. Here, we focus on a family of organometallic complexes with a conjugated curved buckybowl as the ligand. Using first-principles calculations, a multi-mode reversible spin switching based on the CpFe·corannulene complex is predicted by the temperature control of the CpFe+ coordination position in corannulene. The different spin conductance states for three coordinated modes are ascribed to the different electronic spin states of the organometallic complex due to crystal field effects. The predicted relative stabilities of isomers and the energy barriers of isomerization reactions can ensure that the conversion among the three isomers can occur quickly and, at a specific temperature, a dominant isomer has a higher proportion than the other two isomers. This provides a new framework for understanding transport in organometallic complexes with localized d states. This presents an exciting opportunity for exploiting junctions involving molecular spin switching.
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http://dx.doi.org/10.1039/c8cp02914hDOI Listing
August 2018

Boundary value problems for hypergenic function vectors.

J Inequal Appl 2018 13;2018(1):132. Epub 2018 Jun 13.

College of Mathematics and Information Science, Hebei Normal University, Shijiazhuang, P.R. China.

This article mainly studies the boundary value problems for hypergenic function vectors in Clifford analysis. Firstly, some properties of hypergenic quasi-Cauchy type integrals are discussed. Then, by the Schauder fixed point theorem the existence of the solution to the nonlinear boundary value problem is proved. Finally, using the compression mapping principle the existence and uniqueness of the solution to the linear boundary value problem are proved.
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http://dx.doi.org/10.1186/s13660-018-1725-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999220PMC
June 2018

Double-helix PLi chains: novel potential nonlinear optical materials.

Phys Chem Chem Phys 2018 May;20(18):12618-12623

Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.

The structures, circular dichroism (CD) spectra and nonlinear optical (NLO) responses of a series of inorganic double-helix chains, PnLin (n = 6-12), have been investigated using the quantum chemistry method. P-P and P-Li interactions play a major role in stabilizing double-helix chains. The distinctive CD spectra of the double-helix frameworks (namely, a sharp negative CD band at short-wavelength region and a positive CD band at long-wavelength region) become obvious with increasing number of PLi units. The NLO response augments with the length of the double-helix chains, and the contribution of the axial component along the chain direction gradually becomes crucial simultaneously. Synergistic effects, a decrease of crucial electronic transition energies and charge transfer excitation give rise to enhanced NLO responses. In particular, the electronic transitions from the highest occupied molecular orbital to the lowest unoccupied molecular orbital make significant contributions not only to the positive CD bands in the long-wavelength region, but also to the NLO responses of the double-helix PnLin (n = 6-12) chains.
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http://dx.doi.org/10.1039/c8cp01116hDOI Listing
May 2018

All-phosphorus flexible devices with non-collinear electrodes: a first principles study.

Phys Chem Chem Phys 2018 Mar;20(10):7167-7172

College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.

With the continuous expansion of the family of two-dimensional (2D) materials, flexible electronics based on 2D materials have quickly emerged. Theoretically, predicting the transport properties of the flexible devices made up of 2D materials using first principles is of great importance. Using density functional theory combined with the non-equilibrium Green's function formalism, we calculated the transport properties of all-phosphorus flexible devices with non-collinear electrodes, and the results predicted that the device with compressed metallic phosphorene electrodes sandwiching a P-type semiconducting phosphorene shows a better and robust conducting behavior against the bending of the semiconducting region when the angle between the two electrodes is less than 45°, which indicates that this system is very promising for flexible electronics. The calculation of a quantum transport system with non-collinear electrodes demonstrated in this work will provide more interesting information on mesoscopic material systems and related devices.
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http://dx.doi.org/10.1039/c7cp08462eDOI Listing
March 2018

Zoonotic and host-adapted genotypes of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in dairy cattle in Hebei and Tianjin, China.

Vet Parasitol 2017 Dec 2;248:68-73. Epub 2017 Nov 2.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou, China. Electronic address:

A total of 1040 fecal samples, collected from 12 dairy cattle farms in Hebei and Tianjin, near the Bohai area of China, were screened for Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi by polymerase chain reaction. The overall prevalence for Cryptosporidium, G. duodenalis and E. bieneusi was 1.0% (n=10), 4.7% (n=49) and 19.4% (n=202), respectively. Ten Cryptosporidium-positive samples were identified as C. parvum by DNA sequence analysis of the small subunit rRNA (SSU rRNA) gene. DNA sequencing of the 60-kDa glycoprotein gene revealed that the C. parvum samples were all subtype IIdA19G1. Forty-nine G. duodenalis-positive samples belonged to assemblage E (n=47) and assemblage E mixed with A (n=2), based on the sequenced SSU rRNA, triosephosphate isomerase, and glutamate dehydrogenase genes. Sequence analysis of the internal transcribed spacer (ITS) gene identified six known E. bieneusi genotypes, I (n=87), J (n=83), BEB4 (n=18), BEB6 (n=3), N (n=1) and Ebpc (n=1), along with three new genotypes, CHC6 (n=1), CHC7 (n=1) and CHC8 (n=7). Phylogenetic analysis showed that Ebpc belonged to zoonotic group 1, whereas the other E. bieneusi genotypes clustered within group 2. More studies are needed to better understand the species distributions and public health significance of these pathogens in the study areas.
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http://dx.doi.org/10.1016/j.vetpar.2017.10.024DOI Listing
December 2017

Doping Effect of Graphene Nanoplatelets on Electrical Insulation Properties of Polyethylene: From Macroscopic to Molecular Scale.

Materials (Basel) 2016 Aug 10;9(8). Epub 2016 Aug 10.

Key Laboratory of Engineering Dielectric and its Application, Ministry of Education, Harbin University of Science and Technology, Harbin 150040, China.

The doping effect of graphene nanoplatelets (GNPs) on electrical insulation properties of polyethylene (PE) was studied by combining experimental and theoretical methods. The electric conduction properties and trap characteristics were tested for pure PE and PE/GNPs composites by using a direct measurement method and a thermal stimulated current (TSC) method. It was found that doping smaller GNPs is more beneficial to decrease the conductivity of PE/GNPs. The PE/GNPs composite with smaller size GNPs mainly introduces deep energy traps, while with increasing GNPs size, besides deep energy traps, shallow energy traps are also introduced. These results were also confirmed by density functional theory (DFT) and the non-equilibrium Green's function (NEGF) method calculations. Therefore, doping small size GNPs is favorable for trapping charge carriers and enhancing insulation ability, which is suggested as an effective strategy in exploring powerful insulation materials.
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http://dx.doi.org/10.3390/ma9080680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5512346PMC
August 2016