Publications by authors named "Guangxu Zhang"

13 Publications

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Evaluation of the acute toxic effects of crude oil on intertidal mudskipper (Boleophthalmus pectinirostris) based on antioxidant enzyme activity and the integrated biomarker response.

Environ Pollut 2021 Oct 9;292(Pt A):118341. Epub 2021 Oct 9.

School of Marine Sciences, University of Maine, Orono, 04469, USA.

With the development of marine oil industry, oil spill accidents will inevitably occur, further polluting the intertidal zone and causing biological poisoning. The muddy intertidal zone and Boleophthalmus pectinirostris were selected as the research objects to conduct indoor acute exposure experiments within 48 h of crude oil pollution. Statistical analysis was used to reveal the activity changes of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) in the gills and liver of mudskipper. Then, integrated biomarker response (IBR) indicators were established to comprehensively evaluate the biological toxicity. The results showed that the activities of SOD, CAT and GST in livers were higher than those in gills, and the maximum induction multipliers of SOD, CAT and GPx in livers appeared earlier than those in gills. Both SOD and GPx activities were induced at low pollutant concentrations and inhibited at high pollutant concentrations. For the dose-effect, the change trends of CAT and SOD were roughly inversed. There was substrate competition between GPx and CAT, with opposite trends over time. The activating mechanism of GST was similar to that of GPx, and the activation time was earlier than that of GPx. In terms of dose-effect trends, the IBR showed that the antioxidant enzymes activities in biological tissues were induced by low and inhibited by high pollutant concentrations. Overall, SOD and GPx in gills and CAT and GST in livers of the mudskippers were suitable as representative markers to comprehensively analyze and evaluate the biotoxicity effects of oil pollution in the intertidal zone. The star plots and IBR values obtained after data standardization were consistent with the enzyme activity differences, which can be used as valid supplementary indexes for biotoxicity evaluation. These research findings provide theoretical support for early indicators of biological toxicity after crude oil pollution in intertidal zones.
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http://dx.doi.org/10.1016/j.envpol.2021.118341DOI Listing
October 2021

Investigating the critical characteristics of thermal runaway process for LiFePO/graphite batteries by a ceased segmented method.

iScience 2021 Oct 4;24(10):103088. Epub 2021 Sep 4.

Clean Energy Automotive Engineering Center, Tongji University, Shanghai 201804, China.

Lithium-ion batteries (LIBs) are widely used as the energy carrier in our daily life. However, the higher energy density of LIBs results in poor safety performance. Thermal runaway (TR) is the critical problem which hinders the further application of LIBs. Clarifying the mechanism of TR evolution is beneficial to safer cell design and safety management. In this paper, liquid nitrogen spray is proved to be an effective way to stop the violent reaction of LIBs during the TR process. Based on extended-volume accelerating rate calorimetry, the liquid nitrogen ceasing combined with non-atmospheric exposure analysis is used to investigate the TR evolution about LiFePO/graphite batteries at critical temperature. Specifically, the geometrical shape, voltage, and impedance change are monitored during the TR process on the cell level. The morphologies/constitution of electrodes and separators are presented on the component level. Utilizing the gas analysis, the failure mechanism of the prismatic LiFePO/graphite battery is studied comprehensively.
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http://dx.doi.org/10.1016/j.isci.2021.103088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8482432PMC
October 2021

The controllable synthesis of urchin-shaped hierarchical superstructure MOFs with high catalytic activity and stability.

Chem Commun (Camb) 2021 Sep 10;57(70):8758-8761. Epub 2021 Aug 10.

School of Chemical Engineering, The University of Queensland, Brisbane 4072, Australia.

A novel dissolution-crystallization-attachment strategy was developed to synthesize urchin-shaped superstructure metal-organic frameworks (MOFs) with self-assembled one-dimensional nanorods. The superstructure MOFs not only inherited the high activity of nanosized MOFs but also displayed the high stability of microsized MOFs.
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http://dx.doi.org/10.1039/d1cc03547aDOI Listing
September 2021

Comprehensive Investigation of a Slight Overcharge on Degradation and Thermal Runaway Behavior of Lithium-Ion Batteries.

ACS Appl Mater Interfaces 2021 Jul 18;13(29):35054-35068. Epub 2021 Jul 18.

Institute for Advanced Study, Tongji University, Shanghai 200092, China.

Overcharge is a hazardous abuse condition that has dominant influences on cell performance and safety. This work, for the first time, comprehensively investigates the impact of different overcharge degrees on degradation and thermal runaway behavior of lithium-ion batteries. The results indicate that single overcharge has little influence on cell capacity, while it severely degrades thermal stability. Degradation mechanisms are investigated by utilizing the incremental capacity-differential voltage and relaxation voltage analyses. During the slight overcharge process, the conductivity loss and the loss of lithium inventory always occur; the loss of active material starts happening only when the cell is overcharged to a certain degree. Lithium plating is the major cause for the loss of lithium inventory, and an interesting phenomenon that the arrival time of the d/d peak decreases linearly with the increase of the overcharge degree is found. The cells with different degrees of overcharge exhibit a similar behavior during adiabatic thermal runaway. Meanwhile, the relationship between sudden voltage drop and thermal runaway is further established. More importantly, the characteristic temperature of thermal runaway, especially the self-heating temperature (), decreases severely along with overcharging, which means that a slight overcharge severely decreases the cell thermal stability. Further, post-mortem analysis is conducted to investigate the degradation mechanisms. The mechanism of the side reactions caused by a slight overcharge on the degradation performance and thermal runaway characteristics is revealed.
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http://dx.doi.org/10.1021/acsami.1c06029DOI Listing
July 2021

Macrochirality of Self-Assembled and Co-assembled Supramolecular Structures of a Pair of Enantiomeric Peptides.

Front Mol Biosci 2021 23;8:700964. Epub 2021 Jun 23.

Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

Although macrochirality of peptides' supramolecular structures has been found to play important roles in biological activities, how macrochirality is determined by the molecular chirality of the constituted amino acids is still unclear. Here, two chiral peptides, Ac-KHHQKLVFFAK-NH (KK-11) and Ac-KHHQKL VFFAK-NH (KKd-11), which were composed entirely of either L- or D-amino acids, were designed for studying the chiral characteristics of the supramolecular microstructures. It was found that monocomponent KK-11 or KKd-11 self-assembled into right- or left-handed helical nanofibrils, respectively. However, when they co-assembled with concentration ratios varied from 1:9 to 9:1, achiral nanowire-like structures were formed. Both circular dichroism and Fourier transform infrared spectra indicated that the secondary structures changed when the peptides co-assembled. MD simulations indicated that KK-11 or KKd-11 exhibited a strong propensity to self-assemble into right-handed or left-handed nanofibrils, respectively. However, when KK-11 and KKd-11 were both presented in a solution, they had a higher probability to co-assemble instead of self-sort. MD simulations indicated that, in their mixtures, they formed nanowires without handedness feature, a good agreement with experimental observation. Our results shed light on the molecular mechanisms of the macrochirality of peptide supramolecular microstructures.
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http://dx.doi.org/10.3389/fmolb.2021.700964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260686PMC
June 2021

Lysine 164 is critical for SARS-CoV-2 Nsp1 inhibition of host gene expression.

J Gen Virol 2021 01 5;102(1). Epub 2020 Nov 5.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.

The emerging pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused social and economic disruption worldwide, infecting over 9.0 million people and killing over 469 000 by 24 June 2020. Unfortunately, no vaccine or antiviral drug that completely eliminates the transmissible disease coronavirus disease 2019 (COVID-19) has been developed to date. Given that coronavirus nonstructural protein 1 (nsp1) is a good target for attenuated vaccines, it is of great significance to explore the detailed characteristics of SARS-CoV-2 nsp1. Here, we first confirmed that SARS-CoV-2 nsp1 had a conserved function similar to that of SARS-CoV nsp1 in inhibiting host-protein synthesis and showed greater inhibition efficiency, as revealed by ribopuromycylation and Renilla luciferase (Rluc) reporter assays. Specifically, bioinformatics and biochemical experiments showed that by interacting with 40S ribosomal subunit, the lysine located at amino acid 164 (K164) was the key residue that enabled SARS-CoV-2 nsp1 to suppress host gene expression. Furthermore, as an inhibitor of host-protein expression, SARS-CoV-2 nsp1 contributed to cell-cycle arrest in G0/G1 phase, which might provide a favourable environment for virus production. Taken together, this research uncovered the detailed mechanism by which SARS-CoV-2 nsp1 K164 inhibited host gene expression, laying the foundation for the development of attenuated vaccines based on nsp1 modification.
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http://dx.doi.org/10.1099/jgv.0.001513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116783PMC
January 2021

The Corrosion Features of Q235B Steel under Immersion Test and Electrochemical Measurements in Desulfurization Solution.

Materials (Basel) 2020 Aug 27;13(17). Epub 2020 Aug 27.

School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China.

With the continuous tightening marine diesel engines emission standards, removing sulfur oxides (SO) by sodium hydroxide solution absorption is a highly efficiency and economic method, which has been a hot area of research. The ensuing desulfurization solution is a new corrosive system, the aim of this paper is to ascertain the corrosion feature of Q235B steel in desulfurization solution, which lays a theoretical foundation for industrialization. For this purpose, mass loss, electrochemical techniques and surface analyses were applied. The results of mass loss highlight a reduction in the corrosion rate with 35 days of immersion. Higher exposure time increased the compactness of the corrosion product layer and changed phase composition. These conclusions are supported by surface analyses, such as X-ray diffraction and scanning electron microscope. However, electrochemical results showed that the polarization resistance was fluctuant. Both of and charge transfer resistance reach a maximum after immersing 21 days. In addition, although the sediments attached to the steel surface could inhibit corrosion, pitting corrosion aggravated by hydrolyzation of FeSO should be given more attention.
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http://dx.doi.org/10.3390/ma13173783DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504532PMC
August 2020

Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion.

Viruses 2020 07 28;12(8). Epub 2020 Jul 28.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.

Non-structural protein 1 (nsp1) is only characterized in alphacoronaviruses (α-CoVs) and betacoronaviruses (β-CoVs). There have been extensive researches on how the β-CoVs nsp1 regulates viral virulence by inhibiting host protein synthesis, but the regulatory mechanism of the α-CoVs nsp1 is still unclear. Here, we report the 2.1-Å full-length crystal structure of nsp1 in emerging porcine SADS-CoV and the 1.8-Å full-length crystal structure of nsp1 in the highly lethal cat FIPV. Although they belong to different subtypes of α-CoVs, these viruses all have a bucket-shaped fold composed of six β-sheets, similar to the crystal structure of PEDV and TGEV nsp1. Comparing the above four structures, we found that the structure of α-CoVs nsp1 in the same subtype was more conserved. We then selected mammalian cells that were treated with SADS-CoV and FIPV nsp1 for RNA sequencing analysis and found that nsp1 had a specific inhibitory effect on interferon (IFN) and cell cycle genes. Using the Renilla luciferase (Rluc) assay and Western blotting, we confirmed that seven representative α-CoVs nsp1s could significantly inhibit the phosphorylation of STAT1-S727 and interfere with the effect of IFN-I. Moreover, the cell cycle experiment confirmed that α-CoVs nsp1 could encourage host cells to stay in the G0/G1 phase. Based on these findings, we not only greatly improved the crystal structure data on α-CoVs nsp1, but we also speculated that α-CoVs nsp1 regulated host proliferation and immune evasion-related biological functions by inhibiting the synthesis of host proteins, thus creating an environment conducive to the virus.
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http://dx.doi.org/10.3390/v12080812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472224PMC
July 2020

QTL mapping for yield-related traits in wheat based on four RIL populations.

Theor Appl Genet 2020 Mar 2;133(3):917-933. Epub 2020 Jan 2.

State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, China.

Key Message: Eight environmentally stable QTL for grain yield-related traits were detected by four RIL populations, and two of them were validated by a natural wheat population containing 580 diverse varieties or lines. Yield and yield-related traits are important factors in wheat breeding. In this study, four RIL populations derived from the cross of one common parent Yanzhan 1 (a Chinese domesticated cultivar) and four donor parents including Hussar (a British domesticated cultivar) and three semi-wild wheat varieties in China were phenotyped for 11 yield-related traits in eight environments. An integrated genetic map containing 2009 single-nucleotide polymorphism (SNP) markers generated from a 90 K SNP array was constructed to conduct quantitative trait loci (QTL) analysis. A total of 161 QTL were identified, including ten QTL for grain yield per plant (GYP) and yield components, 49 QTL for spike-related traits, 43 QTL for flag leaf-related traits, 22 QTL for plant height (PH), and 37 QTL for heading date and flowering date. Eight environmentally stable QTL were validated in individual RIL population where the target QTL was notably detected, and six of them had a significant effect on GYP. Furthermore, Two QTL, QSPS-2A.4 and QSL-4A.1, were also validated in a natural wheat population containing 580 diverse varieties or lines, which provided valuable resources for further fine mapping and genetic improvement in yield in wheat.
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http://dx.doi.org/10.1007/s00122-019-03515-wDOI Listing
March 2020

Highly efficient absorption of nitric oxide by Ru(edta) aqueous solutions at low concentrations.

Environ Technol 2020 Sep 18;41(21):2705-2715. Epub 2019 Feb 18.

School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan, People's Republic of China.

In this paper, Ru(edta) was used as a highly efficient absorbent for the removal of NO due to its desirable properties of high NO affinity and oxygen insensitivity. The effects of the Ru(edta) concentration, reaction temperature, initial solution pH, oxygen concentration, inlet NO concentration, and liquid-to-gas ratio on denitration performance were examined. The results indicated that Ru(edta) showed excellent denitration performance at low concentrations and that an increase in the concentration of Ru(edta) resulted in an increase in NO removal efficiency. In addition, NO removal efficiency increased to its optimum value at first and then declined as both the reaction temperature and initial solution pH rose. The optimal reaction temperature and ideal initial solution pH were determined to be 45°C and 5.0 respectively. The suitable liquid-to-gas ratio was found to be 51.5 L/m. NO removal efficiency was less affected by the oxygen concentration in the range of 0-12% due to a superior anti-oxidation performance at pH = 5.0. Furthermore, the NO removal efficiency decreased significantly as the inlet NO concentration increased. The addition of 5 wt% urea to an aqueous solution of Ru(edta) enhanced denitration performance, and an Ru(edta) and urea mixed solution were able to exceed 65.07% NO removal efficiency within 30 min under optimal experimental conditions. This work proposed an alternative absorbent for NO removal and provided fundamental data for industrial denitration with Ru(edta) absorbents.
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http://dx.doi.org/10.1080/09593330.2019.1579871DOI Listing
September 2020

Genetic Architecture of Nitrogen-Deficiency Tolerance in Wheat Seedlings Based on a Nested Association Mapping (NAM) Population.

Front Plant Sci 2018 26;9:845. Epub 2018 Jun 26.

State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, China.

Genetic divergence for nitrogen utilization in germplasms is important in wheat breeding programs, especially for low nitrogen input management. In this study, a nested association mapping (NAM) population, derived from "Yanzhan 1" (a Chinese domesticated cultivar) crossed with "Hussar" (a British domesticated cultivar) and another three semi-wild wheat varieties, namely, "Cayazheda 29" ( ssp. Shao), "Yunnan" ( ssp. King), and "Yutian" ( Udats et Migusch), was used to detect quantitative trait loci (QTLs) for nitrogen utilization at the seedling stage. An integrated genetic map was constructed using 2,059 single nucleotide polymorphism (SNP) markers from a 90 K SNP chip, with a total coverage of 2,355.75 cM and an average marker spacing of 1.13 cM. A total of 67 QTLs for RDW (root dry weight), SDW (shoot dry weight), TDW (total dry weight), and RSDW (root to shoot ratio) were identified under normal nitrogen conditions (N) and nitrogen deficient conditions (N). Twenty-three of these QTLs were only detected under N conditions. Moreover, 23 favorable QTLs were identified in the domesticated cultivar Yanzhan 1, 15 of which were detected under N conditions, while only four were detected under N conditions. In contrast, the semi-wild cultivars contributed more favorable N-specific QTLs (eight from Cayazheda 29; nine from Yunnan), which could be further explored for breeding cultivars adapted to nitrogen-deficient conditions. In particular, from YN should be further evaluated using high-resolution mapping.
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http://dx.doi.org/10.3389/fpls.2018.00845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028695PMC
June 2018

TLR-Activated Gap Junction Channels Protect Mice against Bacterial Infection through Extracellular UDP Release.

J Immunol 2016 Feb 18;196(4):1790-8. Epub 2016 Jan 18.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, China

Extracellular UDP (eUDP), released as a danger signal by stressed or apoptotic cells, plays an important role in a series of physiological processes. Although the mechanism of eUDP release in apoptotic cells has been well defined, how the eUDP is released in innate immune responses remains unknown. In this study, we demonstrated that UDP was released in both Escherichia coli-infected mice and LPS- or Pam3CSK4-treated macrophages. Also, LPS-induced UDP release could be significantly blocked by selective TLR4 inhibitor Atractylenolide I and selective gap junction inhibitors carbenoxolone and flufenamic acid (FFA), suggesting the key role of TLR signaling and gap junction channels in this process. Meanwhile, eUDP protected mice from peritonitis by reducing invaded bacteria that could be rescued by MRS2578 (selective P2Y6 receptor inhibitor) and FFA. Then, connexin 43, as one of the gap junction proteins, was found to be clearly increased by LPS in a dose- and time-dependent manner. Furthermore, if we blocked LPS-induced ERK signaling by U0126, the expression of connexin 43 and UDP release was also inhibited dramatically. In addition, UDP-induced MCP-1 secretion was significantly reduced by MRS2578, FFA, and P2Y6 mutation. Accordingly, pretreating mice with U0126 and Gap26 increased invaded bacteria and aggravated mice death. Taken together, our study reveals an internal relationship between danger signals and TLR signaling in innate immune responses, which suggests a potential therapeutic significance of gap junction channel-mediated UDP release in infectious diseases.
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http://dx.doi.org/10.4049/jimmunol.1501629DOI Listing
February 2016

Molecular characterization of RIG-I, STAT-1 and IFN-beta in the horseshoe bat.

Gene 2015 Apr 10;561(1):115-23. Epub 2015 Feb 10.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-β in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-β showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-β was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection.
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http://dx.doi.org/10.1016/j.gene.2015.02.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127221PMC
April 2015
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