Publications by authors named "Guanglei Wang"

29 Publications

  • Page 1 of 1

Matrine ameliorates the inflammatory response and lipid metabolism in vascular smooth muscle cells through the NF-κB pathway.

Exp Ther Med 2021 Nov 16;22(5):1309. Epub 2021 Sep 16.

Department of Emergency Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, P.R. China.

Atherosclerosis is a chronic inflammatory disease associated with inflammatory responses and the uncontrolled proliferation and excessive apoptosis of vascular smooth muscle cells. However, the effects of matrine on the inflammatory response, abnormal lipid metabolism and cell proliferation and apoptosis marker proteins in human aortic vascular smooth muscle cells (HAVSMCs) have not been elucidated. Therefore, the present study aimed to investigate the effect of matrine on an model of atherosclerosis using HAVSMCs. The HAVSMCs were divided into normal, model and matrine groups. The model group was treated with oxidized low-density lipoprotein (oxLDL), the matrine group was treated with oxLDL and matrine and the normal group was treated with physiological saline. Total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) levels were measured in the cell supernatant. In addition, the relative mRNA levels of inflammatory factors were quantified using reverse transcription-quantitative PCR, and the cell proliferation and apoptosis rates were evaluated using Cell Counting Kit-8 and flow cytometry assays, respectively. The expression levels of proteins associated with proliferation and apoptosis were also determined using western blotting. The levels of TC, FC and CE and the mRNA levels of IL-1β, IL-6, and TNF-α in the matrine group were lower than those in the model group, but higher than those in the normal group. After 48 and 96 h of treatment, the cell proliferation and apoptotic rates were lower in the matrine group compared with the model group. The relative expression levels of Ki-67, proliferating cell nuclear antigen and Bax were decreased, while that of Bcl-2 was increased in the matrine group compared with the model group. In addition, the relative protein expression of nuclear factor κB (NF-κB) in the matrine group was lower than that in the model group, but higher than that in the normal group. In summary, matrine inhibited activation of the NF-κB pathway and reduced cell proliferation and apoptosis in the oxLDL-induced atherosclerosis model, and exhibited anti-inflammatory effects. These results suggest that matrine attenuated abnormal biological reactions in HAVSMCs through the NF-κB pathway.
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http://dx.doi.org/10.3892/etm.2021.10744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461614PMC
November 2021

The Effect of Lidocaine on Postoperative Quality of Recovery and Lung Protection of Patients Undergoing Thoracoscopic Radical Resection of Lung Cancer.

Drug Des Devel Ther 2021 7;15:1485-1493. Epub 2021 Apr 7.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, People's Republic of China.

Purpose: To evaluate the effectiveness and safety of lidocaine on postoperative quality of recovery and lung protection of patients undergoing thoracoscopic radical resection of lung cancer.

Patients And Methods: Seventy ASA II-III patients undergoing thoracoscopic radical resection of lung cancer were randomly assigned into either the lidocaine group (Group L) or control group (Group C). Patients in Group L received lidocaine with a 1.5 mg/kg bolus before induction of anesthesia, followed by 2.0 mg/kg/h until the end of the operation while the patients in Group C received volume-matched normal saline at the same rate. The main outcome was the quality of recovery-40 score (QoR-40 score) at 24 h postoperatively. The peak airway pressure (Ppeak) and plateau airway pressure (Pplat), the partial pressure of oxygen in arterial blood (PaO), partial pressure of carbon dioxide in arterial blood (PaCO), alveolar-arterial oxygen gradient (A-aDO2), oxygenation index (OI), time to first flatus and defecation, intraoperative hemodynamics and opioid consumption were also recorded.

Results: There were no statistically difference at patients' baseline characteristics. The QoR-40 score of Group L was significantly higher than that of Group C at 24 h after surgery (=0.014). Ppeak, Pplat, and A-aDO of Group L were significantly lower than those of Group C (<0.001, <0.001, =0.025, respectively) after the ventilation recovery of both lungs, and the PaO and OI of the Group L were significantly higher than those of Group C (=0.027, =0.027, respectively). Time to first flatus and defecation in Group L was significantly lower compared with Group C (=0.037, =0.025, respectively).

Conclusion: Intravenous lidocaine can improve the quality of recovery of patients undergoing thoracoscopic radical resection of lung cancer, while also providing lung protection, favorable postoperative analgesia, a reduction in the time to first flatus and defecation after surgery.
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http://dx.doi.org/10.2147/DDDT.S297642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039043PMC
April 2021

Diagnostic value and prognostic significance of CTRP9 combined with pentraxin-3 in acute coronary syndrome.

Exp Ther Med 2021 Mar 25;21(3):254. Epub 2021 Jan 25.

Department of Cardiology, Dongying Hospital of Traditional Chinese Medicine, Dongying, Shandong 257000, P.R. China.

The present study aimed to explore the diagnostic value and prognostic significance of C1q/tumor necrosis factor-related protein 9 (CTRP9) combined with pentraxin-3 (PTX-3) in acute coronary syndrome (ACS). A total of 137 patients with coronary heart disease and chest pain were included. Among them, seventy-nine patients with ACS were allocated into a study group and fifty-eight patients with non-cardiac chest pain (NCCP) were allocated into a control group. The serum CTRP9, PTX-3 levels were quantified by ELISA, and their correlation with other ACS-related indexes, diagnostic value for ACS and predictive significance for poor prognosis were analyzed. In addition, the risk factors of the poor prognosis of ACS patients were studied. CTRP9 was lowly expressed and PTX-3 was highly expressed in the serum of ACS patients. CTRP9 was negatively correlated with cardiac troponin I (cTnI), creatine kinase-MB (CK-MB) and high-sensitivity C-reactive protein (hs-CRP) (P<0.05), while PTX-3 was positively correlated with them (P<0.05). Combined detection of CTRP9 and PTX-3 was of high value in the diagnosis and prognosis of ACS patients. In addition, CTRP9 and PTX-3 were independent risk factors for the poor prognosis of ACS. Patients with ACS had lower CTRP9 expression and higher PTX-3 expression than those without ACS. Moreover, the combined detection of CTRP9 and PTX-3 can better evaluate the diagnosis and prognosis of ACS patients.
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http://dx.doi.org/10.3892/etm.2021.9685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851676PMC
March 2021

Effects of menstrual cycle on nausea and vomiting after general anesthesia.

J Anesth 2020 08 27;34(4):519-526. Epub 2020 Apr 27.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, People's Republic of China.

Purpose: To investigate the impact of menstrual cycle on patients undergoing gynecological endoscopic surgery.

Patients And Methods: 220 patients scheduled for gynecological endoscopic surgery under general anesthesia were selected. The patients were divided into three groups based on 3 phases of menstrual cycle which are the follicular phase (Group F), ovulatory phase (Group O) or luteal phase (Group L). It is based on their duration of menstruation and the last day of menstrual bleeding from the date of surgery. Primary outcomes were the incidences of early and late postoperative nausea and vomiting (PONV) in the three patient groups. Preoperative venous blood was taken to determine the estrogen and progesterone levels of the patients.

Results: A total of 207 patients were enrolled. The incidence of early PONV was highest in group O (22.22% vs 43.33% vs 17.86%, P < 0.01). Multivariate logistic regression showed that menstrual cycle (P < 0.01) and sufentanil dosage (P < 0.05) were independent risk factors for early PONV, menstrual cycle (P = 0.03) and intraoperative hypotension (P = 0.03) were independent risk factors for late PONV. After the propensity matching, the incidences of early and late PONV in group O were both higher than that in other two groups (19.23% vs 44.68% vs 16.90%, P < 0.01; 53.80% vs 72.34% vs 45.07%, P = 0.01).

Conclusion: The incidence of PONV after gynecological endoscopic surgery was different in patients with different menstrual cycles, with the highest incidence in ovulation.
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http://dx.doi.org/10.1007/s00540-020-02781-zDOI Listing
August 2020

[Lung nodule segmentation based on fuzzy c-means clustering and improved random walk algorithm].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2019 Dec;36(6):978-985

College of Eletronic and Information Engineering, Hebei University, Baoding, Hebei 071002,

Accurate segmentation of pulmonary nodules is an important basis for doctors to determine lung cancer. Aiming at the problem of incorrect segmentation of pulmonary nodules, especially the problem that it is difficult to separate adhesive pulmonary nodules connected with chest wall or blood vessels, an improved random walk method is proposed to segment difficult pulmonary nodules accurately in this paper. The innovation of this paper is to introduce geodesic distance to redefine the weights in random walk combining the coordinates of the nodes and seed points in the image with the space distance. The improved algorithm is used to achieve the accurate segmentation of pulmonary nodules. The computed tomography (CT) images of 17 patients with different types of pulmonary nodules were selected for segmentation experiments. The experimental results are compared with the traditional random walk method and those of several literatures. Experiments show that the proposed method has good accuracy in the segmentation of pulmonary nodule, and the accuracy can reach more than 88% with segmentation time is less than 4 seconds. The results could be used to assist doctors in the diagnosis of benign and malignant pulmonary nodules and improve clinical efficiency.
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http://dx.doi.org/10.7507/1001-5515.201811056DOI Listing
December 2019

Comparison of short and long axis ultrasound-guided approaches to internal jugular vein puncture: a meta-analysis.

J Int Med Res 2019 Sep 20;47(9):4069-4082. Epub 2019 Aug 20.

Department of Anaesthesiology, Tangshan People's Hospital, North China University of Science and Technology, Tangshan, Hebei, China.

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http://dx.doi.org/10.1177/0300060519867806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753526PMC
September 2019

Effect of dexmedetomidine on etomidate-induced myoclonus: a randomized, double-blind controlled trial.

Drug Des Devel Ther 2019 27;13:1803-1808. Epub 2019 May 27.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, People's Republic of China.

Etomidate used for the induction of general anesthesia can result in myoclonus. We tested the hypothesis that pretreatment with dexmedetomidine (Dex) reduces the incidence of etomidate-induced myoclonus during the induction of general anesthesia. One hundred patients who were scheduled for selective operations under general anesthesia were included in this randomized, double-blind controlled trial. Patients were randomized to receive either Dex 0.5 µg/kg in 20 mL of normal saline or the same volume of normal saline as pretreatment agents 15 mins before the injection of etomidate 0.3 mg/kg. The primary endpoint was the incidence of etomidate-induced myoclonus. Secondary endpoints were the severity of etomidate-induced myoclonus and the incidence of adverse effects from the onset of action of Dex or normal saline to the injection of etomidate, such as dizziness, respiratory depression, bradycardia, hypotension and nausea/vomiting. All of the 100 patients completed the trial. Dex resulted in a significant 38% reduction in the number of patients who experienced etomidate-induced myoclonus: 13 (26%) vs 32 (64%) (=0.0001). Additionally, the severity of myoclonus was also reduced in the Dex group than that in the placebo group (=0.02). Incidence of dizziness, respiratory depression, bradycardia, hypotension and nausea/vomiting was similar in both groups. Pretreatment with Dex 0.5 µg/kg 15 mins before the induction of general anesthesia not only resulted in a 38% reduction in the incidence of etomidate-induced myoclonus, but also reduced the severity of myoclonus, without inducing any adverse effects.
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http://dx.doi.org/10.2147/DDDT.S194456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554000PMC
January 2020

Effect of butorphanol on etomidate-induced myoclonus: a systematic review and meta-analysis.

Drug Des Devel Ther 2019 16;13:1213-1220. Epub 2019 Apr 16.

Department of Anesthesiology, Tangshan People's Hospital, North China University of Science and Technology, Tangshan, Hebei, People's Republic of China.

To evaluate the effect of butorphanol on the prevention of myoclonus induced by etomidate. We searched the PubMed, Embase, Cochrane Library, and China National Knowledge Infrastructure databases to collect relevant randomized controlled trials (RCTs) evaluating the effect of butorphanol on etomidate-induced myoclonus in January 2019 without any language restrictions. The primary outcome was the incidence of etomidate-induced myoclonus. Secondary outcomes included the incidence of myoclonus at various degrees and the incidence of adverse effects. Risk ratios (RRs) were calculated for binary outcomes. All statistical analysis were performed by using RevMan 5.3 software. We identified 6 RCTs involving a total of 608 patients who reported the incidence of etomidate-induced myoclonus. In pooled analyses, the incidence of etomidate-induced myoclonus in the butorphanol group was significantly lower than that in the control group (RR =0.15, 95% CI [0.10, 0.22], <0.00001). Subgroup analyses showed that butorphanol significantly decreased the numbers of patients with mild myoclonus (RR =0.41, 95% CI [0.25, 0.68], =0.0005), moderate myoclonus (RR =0.18, 95% CI [0.09, 0.34], <0.00001), and severe myoclonus (RR =0.04, 95% CI [0.01, 0.10], 0.00001). Additionally, butorphanol did not increase the incidence of postoperative nausea/vomiting (RR =3.0, 95% CI [0.32, 28.42], =0.34) or dizziness (RR =6.79, 95% CI [0.84, 54.84], =0.07) associated with etomidate. Our findings suggest that butorphanol can effectively prevent the incidence of etomidate-induced myoclonus and alleviate the intensity of etomidate-induced myoclonus, without inducing postoperative nausea/vomiting and dizziness.
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http://dx.doi.org/10.2147/DDDT.S191982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489683PMC
December 2019

High-content analysis of particulate matters-induced oxidative stress and organelle dysfunction in vitro.

Toxicol In Vitro 2019 Sep 25;59:263-274. Epub 2019 Apr 25.

School of Public Health, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 29 Nanwei Road, Beijing 100050, China. Electronic address:

Oxidative stress is usually considered to be a common mechanism by which particulate matter (PM) exposure induces adverse effects. However, the further biological events such as organelle dysfunction following oxidative stress remain to be explored. In this study, we applied high-content screening (HCS) technique to investigate the toxicological effects of carbon black (CB), diesel exhaust particle (DEP) and PM2.5 on oxidative stress and organelle function in human bronchial epithelial cell (16HBE), human embryo lung fibroblast cell (HELF) and human umbilical vein endothelial cell (HUVEC) which were used to represent distinct regions of the lung, and compared the toxicity impacts of different PMs and the sensitiveness of cell lines. We found three types of PMs induced mitochondrial dysfunction in three cell lines and lysosomal alkalinization in HUVEC while only CB triggered endoplasmic reticulum (ER) stress in 16HBE and HUVEC, and oxidative stress might mediate these processes. Moreover, CB basically exhibited more potent toxicity compared with DEP and PM2.5, which might be attributed to its less oxygen content. Finally, the finding that PMs-induced toxicity impacts exhibited a cell-type dependent manner might provide some information to help to understand the sensitivity of different tissue in the lung.
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http://dx.doi.org/10.1016/j.tiv.2019.04.026DOI Listing
September 2019

[Automatic multi-region segmentation of intracoronary optical coherence tomography images based on neutrosophic theory].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2019 Feb;36(1):59-67

College of Eletronic and Information Engineering, Hebei University, Baoding, Hebei 071002, P.R.China.

Optical coherence tomography (OCT) has become a key technique in the diagnosis of coronary artery stenosis, which can identify plaques and vulnerable plaques in the image. Therefore, this technique is of great significance for the diagnosis of coronary heart disease. However, there is still a lack of automatic, multi-region, high-precision segmentation algorithms for coronary OCT images in the current research field. Therefore, this paper proposes a multi-zone, fully automated segmentation algorithm for coronary OCT images based on neutrosophic theory, which achieves high-precision segmentation of fibrous plaques and lipid regions. In this paper, the method of transforming OCT images into T in the area of neutrosophics is redefined based on the membership function, and the segmentation accuracy of fiber plaques is improved. For the segmentation of lipid regions, the algorithm adds homomorphic filter enhancement images, and uses OCT to transform OCT images into I in the field of neutrosophics, and further uses morphological methods to achieve high-precision segmentation. In this paper, 40 OCT images from 9 patients with typical plaques were analyzed and compared with the results of manual segmentation by doctors. Experiments show that the proposed algorithm avoids the over-segmentation and under-segmentation problems of the traditional neutrosophic theory method, and accurately segment the patch area. Therefore, the work of this paper can effectively improve the accuracy of segmentation of plaque for doctors, and assist clinicians in the diagnosis and treatment of coronary heart disease.
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http://dx.doi.org/10.7507/1001-5515.201804035DOI Listing
February 2019

Time-course effects of antioxidants and phase II enzymes on diesel exhaust particles-induced oxidative damage in the mouse lung.

Toxicol Appl Pharmacol 2019 03 12;366:25-34. Epub 2019 Jan 12.

School of Public Health, Qingdao University, Dengzhou Road 38, Qingdao 266021, Shandong Province, China; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Xicheng District, 29 Nanwei Road, Beijing 100050, China. Electronic address:

Mechanisms responsible for diesel exhaust particle (DEP)-induced toxicity in respiratory disorders are poorly understood, recent experimental and controlled exposure studies suggested that oxidative stress might be involved. To investigate the time-course effects DEP on nuclear factor erythroid 2-related factor 2 (Nrf2), a key regulator in cellular adaptive antioxidant response, mice were intratracheal instilled with 100 μg DEP/mouse and sacrificed after 30 min, 6 h, 12 h, 24 h, 48 h, and 72 h. We measured reactive oxygen species (ROS) as well as Nrf2 and antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and phase II enzymes including heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase-1 (NQO1), glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM) in the lungs. Additionally, histopathological changes were examined. At 6 h, ROS peaked, most of the enzymes were activated, and the histology showed the lungs were damaged. At 12 h, ROS returned to normal level and CAT activity decreased, while protein expression of Nrf2, HO-1, NQO1, GCLC, and GCLM increased, and the lungs were recovering from damage. After 24 h, ROS started to decrease and Nrf2 showed a decreasing trend at both gene and protein levels, while the lung damage had been entirely restored. These results suggested that a single exposure to DEP induce transient oxidative stress in the lungs, with time-dependent effects on Nrf2 and antioxidant enzymes and phase II enzymes.
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http://dx.doi.org/10.1016/j.taap.2019.01.010DOI Listing
March 2019

Effect of intrathecal dexmedetomidine on preventing shivering in cesarean section after spinal anesthesia: a meta-analysis and trial sequential analysis.

Drug Des Devel Ther 2018 2;12:3775-3783. Epub 2018 Nov 2.

Department of Anesthesiology, The Affiliated Hospital of XuZhou Medical University, XuZhou, JiangSu, China,

Objective: Intrathecal dexmedetomidine (DEX) has been used to prevent shivering in patients undergoing cesarean section. The aim of this meta-analysis was to evaluate whether intrathecal DEX could prevent shivering in cesarean section after spinal anesthesia.

Methods: We searched PubMed, Embase, Web of Science, and the Cochrane Library for randomized controlled trials (RCTs) comparing intrathecal DEX in cesarean section after spinal anesthesia with placebo and reporting on shivering, postoperative nausea and vomiting (PONV), hypotension, and bradycardia. Trial sequential analysis (TSA) was also carried out for RCTs comparing DEX with placebo. This meta-analysis has been registered on PROSPERO, and the registration number is CRD42017071640.

Results: Six randomized clinical trials comparing 360 patients were included in this study. Compared with placebo, intrathecal DEX significantly reduced the incidence of shivering (risk ratio [RR]=0.40; 95% CI [0.26, 0.62]; <0.0001). No significant difference was found in the incidence of PONV (RR=1.34; 95% CI [0.82, 2.18]; =0.24), hypotension (RR=1.09; 95% CI [0.84, 1.42]; =0.50), or bradycardia (RR=1.55; 95% CI [0.54, 4.42]; =0.42). However, no firm conclusions can be made based on the results of all outcomes according to the TSA.

Conclusion: This meta-analysis found that intrathecal DEX could prevent shivering in cesarean section after spinal anesthesia and did not induce PONV, hypotension, or bradycardia. However, firm conclusions cannot be made until more studies are conducted.
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http://dx.doi.org/10.2147/DDDT.S178665DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223340PMC
March 2019

Effect of butorphanol on opioid-induced cough: a meta-analysis of randomized controlled trials.

Drug Des Devel Ther 2018 2;12:3263-3268. Epub 2018 Oct 2.

Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China,

Objective: The aim of this study was to assess the effect of butorphanol on the prevention of opioid-induced cough by a meta-analysis.

Materials And Methods: We searched PubMed, Embase, The Cochrane Library, and the China National Knowledge Infrastructure database for relevant randomized controlled trials (RCTs) to demonstrate the efficacy of butorphanol on the prevention of opioid-induced cough. We used RevMan 5.3 to conduct a meta-analysis on each outcome.

Results: Eight RCTs comparing 942 patients were included in this study. The pooled meta-analysis showed that the incidence of opioid-induced cough in the butorphanol group was significantly decreased compared with that of the control group (risk ratio [RR]=0.17, 95% CI [0.09, 0.33], <0.00001). Incidences of opioid-induced cough in the butorphanol group resulting in mild cough (RR=0.30, 95% CI [0.11, 0.78], =0.01), moderate cough (RR=0.08, 95% CI [0.03, 0.22], <0.00001), or severe cough (RR=0.08, 95% CI [0.02, 0.30], =0.0001) were significantly lower than those of the control group.

Conclusion: This meta-analysis suggested that butorphanol can effectively prevent the incidence of opioid-induced cough and reduce the severity of opioid-induced cough.
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http://dx.doi.org/10.2147/DDDT.S180533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173179PMC
February 2019

LIN28B/let-7 axis mediates pulmonary inflammatory response induced by diesel exhaust particle exposure in mice.

Toxicol Lett 2018 Dec 29;299:1-10. Epub 2018 Aug 29.

School of Public Health, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China. Electronic address:

Exposure to diesel exhaust particle (DEP) is closely related to inflammatory response in respiratory system. To understand the underlying molecular mechanism by which DEP induces pulmonary inflammatory response, we conducted DEP exposure experiments in vivo and in vitro. In vivo, each mouse was exposed to DEP suspension (100 μg of DEP) or vehicle only once in single intra-tracheal instillation (IT) section, or was exposed to DEP suspension (12.5 μg or 50 μg of DEP) or vehicle 12 times in repeated IT section. DEP exposure induced significant pathological injuries with substantial neutrophils infiltration and the increased level of pro-inflammatory cytokine IL-6 in mouse lungs. Consistently, elevated IL6 mRNA level was also observed in DEP treatment group (100 μg/ml) in vitro. In addition, DEP exposure exerted the similar influence on the expression of let-7d and let-7g microRNAs in vivo and in vitro. To verify the possible role of LIN28B/let-7 axis in the regulation of IL6 expression following DEP exposure, we applied RNAi technology in vitro, and found increased IL6 mRNA expression was alleviated or neutralized in DEP exposure groups after LIN28B silencing or after let-7d or let-7g over-expression. Taken together, we conclude that LIN28B/let-7 axis might be involved in inflammatory response induced by DEP exposure.
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http://dx.doi.org/10.1016/j.toxlet.2018.08.019DOI Listing
December 2018

[Plaque segmentation of intracoronary optical coherence tomography images based on -means and improved random walk algorithm].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2017 Jun;34(6):869-875

College of Eletronic and Information Engineering, Hebei University, Baoding, Hebei 071002, P.R.China.

In recent years, optical coherence tomography (OCT) has developed into a popular coronary imaging technology at home and abroad. The segmentation of plaque regions in coronary OCT images has great significance for vulnerable plaque recognition and research. In this paper, a new algorithm based on -means clustering and improved random walk is proposed and Semi-automated segmentation of calcified plaque, fibrotic plaque and lipid pool was achieved. And the weight function of random walk is improved. The distance between the edges of pixels in the image and the seed points is added to the definition of the weight function. It increases the weak edge weights and prevent over-segmentation. Based on the above methods, the OCT images of 9 coronary atherosclerotic patients were selected for plaque segmentation. By contrasting the doctor's manual segmentation results with this method, it was proved that this method had good robustness and accuracy. It is hoped that this method can be helpful for the clinical diagnosis of coronary heart disease.
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http://dx.doi.org/10.7507/1001-5515.201706030DOI Listing
June 2017

Safety and efficacy of the oblique-axis plane in ultrasound-guided internal jugular vein puncture: A meta-analysis.

J Int Med Res 2018 Jul 5;46(7):2587-2594. Epub 2018 Apr 5.

1 Department of Anesthesiology, The Affiliated Hospital of XuZhou Medical University, XuZhou, Jiangsu, China.

Objective This meta-analysis was performed to evaluate the safety and efficacy of the oblique-axis plane in ultrasound-guided internal jugular vein puncture. Methods We searched Embase, PubMed, the Cochrane Library, Web of Science, and China National Knowledge Infrastructure for relevant randomized clinical trials comparing the oblique axis with the short axis in ultrasound-guided internal jugular vein puncture. Results Five randomized clinical trials were included in this meta-analysis. The pooled meta-analysis showed that the incidence of arterial puncture in the oblique-axis group was significantly lower than that in the short-axis group. No significant difference was found in the first-pass success rate between the oblique-axis group and short-axis group. Additionally, there were no significant differences in the puncture success rate or number of attempts required between the two groups. Conclusion Ultrasound-guided internal jugular vein puncture using the oblique-axis plane reduced the risk of arterial puncture, but no difference was found in the first-pass success rate, puncture success rate, or number of attempts required.
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http://dx.doi.org/10.1177/0300060518765344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124295PMC
July 2018

Photodissociation dynamics of HO at 111.5 nm by a vacuum ultraviolet free electron laser.

J Chem Phys 2018 Mar;148(12):124301

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, 457 Zhongshan Road, Dalian, Liaoning 116023, People's Republic of China.

Photodissociation dynamics of HO via the F̃ state at 111.5 nm were investigated using the high resolution H-atom Rydberg "tagging" time-of-flight (TOF) technique, in combination with the tunable vacuum ultraviolet free electron laser at the Dalian Coherent Light Source. The product translational energy distributions and angular distributions in both parallel and perpendicular directions were derived from the recorded TOF spectra. Based on these distributions, the quantum state distributions and angular anisotropy parameters of OH (X) and OH (A) products have been determined. For the OH (A) + H channel, highly rotationally excited OH (A) products have been observed. These products are ascribed to a fast direct dissociation on the B̃A state surface after multi-step internal conversions from the initial excited F̃ state to the B̃ state. While for the OH (X) + H channel, very highly rotationally excited OH (X) products with moderate vibrational excitation are revealed and attributed to the dissociation via a nonadiabatic pathway through the well-known two conical intersections between the B̃-state and the X̃-state surfaces.
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http://dx.doi.org/10.1063/1.5022108DOI Listing
March 2018

Emergence, evolution, and control of multistability in a hybrid topological quantum/classical system.

Chaos 2018 Mar;28(3):033601

School of Electrical, Computer, and Energy Engineering, Arizona State University, Tempe, Arizona 85287, USA.

We present a novel class of nonlinear dynamical systems-a hybrid of relativistic quantum and classical systems and demonstrate that multistability is ubiquitous. A representative setting is coupled systems of a topological insulator and an insulating ferromagnet, where the former possesses an insulating bulk with topologically protected, dissipationless, and conducting surface electronic states governed by the relativistic quantum Dirac Hamiltonian and the latter is described by the nonlinear classical evolution of its magnetization vector. The interactions between the two are essentially the spin transfer torque from the topological insulator to the ferromagnet and the local proximity induced exchange coupling in the opposite direction. The hybrid system exhibits a rich variety of nonlinear dynamical phenomena besides multistability such as bifurcations, chaos, and phase synchronization. The degree of multistability can be controlled by an external voltage. In the case of two coexisting states, the system is effectively binary, opening a door to exploitation for developing spintronic memory devices. Because of the dissipationless and spin-momentum locking nature of the surface currents of the topological insulator, little power is needed for generating a significant current, making the system appealing for potential applications in next generation of low power memory devices.
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http://dx.doi.org/10.1063/1.4998244DOI Listing
March 2018

[Plaque region segmentation of intracoronary optical cohenrence tomography images based on kernel graph cuts].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2017 Feb;34(1):15-20

The segmentation of the intracoronary optical coherence tomography(OCT) images is the basis of the plaque recognition, and it is important to the following plaque feature analysis, vulnerable plaque recognition and further coronary disease aided diagnosis. This paper proposes an algorithm about multi region plaque segmentation based on kernel graph cuts model that realizes accurate segmentation of fibrous, calcium and lipid pool plaques in coronary OCT image, while boundary information has been well reserved. We segmented 20 coronary images with typical plaques in our experiment, and compared the plaque regions segmented by this algorithm to the plaque regions obtained by doctor’s manual segmentation. The results showed that our algorithm is accurate to segment the plaque regions. This work has demonstrated that it can be used for reducing doctors’ working time on segmenting plaque significantly, reduce subjectivity and differences between different doctors, assist clinician’s diagnosis and treatment of coronary artery disease.
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February 2017

The efficacy of pediatric ultrasound guided brachial plexus block anesthesia and determination of optimal anesthetic drug dosage.

Minerva Pediatr 2016 Nov 9. Epub 2016 Nov 9.

Department of Anesthesiology, Tai'an Maternity and Children Health Care Hospital, Shandong Province, China -

Background: The objective of the present study was to investigate the effect of pediatric ultrasound guided brachial plexus block anesthesia and to determine the optimal anesthetic drug dosage.

Methods: Ninety-seven children who required elective upper limb surgery were randomly divided into three groups: Group A with 32 cases, Group B with 35 cases and Group C with 30 cases. All three groups of patients underwent ultrasound guided brachial plexus block anesthesia. To compare differences of anesthetic effect, Group A received 0.30% ropivacaine, Group B received 0.40% ropivacaine, and Group C received 0.50% ropivacaine.

Results: The total volume was 24 ml in each group. The onset time of ulnar nerve block in Group A was significantly longer than in Group B and Group C (P<0.05); there were no significant differences in onset time of nerve block of the other nerves among the three groups (P>0.05). Block maintenance time in Group A was significantly less than in Group B and Group C (P<0.05). The effective rate of anesthesia of Group A was lower than in Group B and Group C and the differences were statistically significant (P<0.05). There was no significant difference in the incidence of anesthetic complications among the three groups (P>0.05).

Conclusions: Pediatric ultrasound guided brachial plexus block anesthesia is safe and reliable. Ropivacaine used at 0.40% is the optimal lower-concentration anesthetic dosage.
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November 2016

Transient chaos - a resolution of breakdown of quantum-classical correspondence in optomechanics.

Sci Rep 2016 10 17;6:35381. Epub 2016 Oct 17.

Institute for Complex Systems and Mathematical Biology, King's College, University of Aberdeen, Aberdeen AB24 3UE, UK.

Recently, the phenomenon of quantum-classical correspondence breakdown was uncovered in optomechanics, where in the classical regime the system exhibits chaos but in the corresponding quantum regime the motion is regular - there appears to be no signature of classical chaos whatsoever in the corresponding quantum system, generating a paradox. We find that transient chaos, besides being a physically meaningful phenomenon by itself, provides a resolution. Using the method of quantum state diffusion to simulate the system dynamics subject to continuous homodyne detection, we uncover transient chaos associated with quantum trajectories. The transient behavior is consistent with chaos in the classical limit, while the long term evolution of the quantum system is regular. Transient chaos thus serves as a bridge for the quantum-classical transition (QCT). Strikingly, as the system transitions from the quantum to the classical regime, the average chaotic transient lifetime increases dramatically (faster than the Ehrenfest time characterizing the QCT for isolated quantum systems). We develop a physical theory to explain the scaling law.
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http://dx.doi.org/10.1038/srep35381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066317PMC
October 2016

Nonlinear dynamics induced anomalous Hall effect in topological insulators.

Sci Rep 2016 Jan 28;6:19803. Epub 2016 Jan 28.

School of Electrical, Computer, and Energy Engineering, Arizona State University, Tempe, AZ 85287, USA.

We uncover an alternative mechanism for anomalous Hall effect. In particular, we investigate the magnetisation dynamics of an insulating ferromagnet (FM) deposited on the surface of a three-dimensional topological insulator (TI), subject to an external voltage. The spin-polarised current on the TI surface induces a spin-transfer torque on the magnetisation of the top FM while its dynamics can change the transmission probability of the surface electrons through the exchange coupling and hence the current. We find a host of nonlinear dynamical behaviors including multistability, chaos, and phase synchronisation. Strikingly, a dynamics mediated Hall-like current can arise, which exhibits a nontrivial dependence on the channel conductance. We develop a physical understanding of the mechanism that leads to the anomalous Hall effect. The nonlinear dynamical origin of the effect stipulates that a rich variety of final states exist, implying that the associated Hall current can be controlled to yield desirable behaviors. The phenomenon can find applications in Dirac-material based spintronics.
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http://dx.doi.org/10.1038/srep19803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730160PMC
January 2016

Conductance stability in chaotic and integrable quantum dots with random impurities.

Phys Rev E Stat Nonlin Soft Matter Phys 2015 Aug 3;92(2):022901. Epub 2015 Aug 3.

School of Electrical, Computer, and Energy Engineering, Arizona State University, Tempe, Arizona 85287, USA.

For a quantum dot system of fixed geometry, in the presence of random impurities the average conductance over an appropriate range of the Fermi energy decreases as the impurity strength is increased. Can the nature of the corresponding classical dynamics in the dot region affect the rate of decrease? Utilizing graphene quantum dots with two semi-infinite, single-mode leads as a prototypical model, we address the device stability issue by investigating the combined effects of classical dynamics and impurities on the average conductance over the energy range of the first transverse mode. We find that, for chaotic dot systems, the rate of decrease in the average conductance with the impurity strength is in general characteristically smaller than that for integrable dots. We develop a semiclassical analysis for the phenomenon and also obtain an understanding based on the random matrix theory. Our results demonstrate that classical chaos can generally lead to a stronger stability in the device performance, strongly advocating exploiting chaos in the development of nanoscale quantum transport devices.
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http://dx.doi.org/10.1103/PhysRevE.92.022901DOI Listing
August 2015

Automatic diagnosis of premature ventricular contraction based on Lyapunov exponents and LVQ neural network.

Comput Methods Programs Biomed 2015 Oct 9;122(1):47-55. Epub 2015 Jul 9.

Affiliated Hospital of Hebei University, Baoding, China.

Premature ventricular contraction (PVC) is a common type of abnormal heartbeat. Without early diagnosis and proper treatment, PVC may result in serious harms. Diagnosis of PVC is of great importance in goal-directed treatment and preoperation prognosis. This paper proposes a novel diagnostic method for PVC based on Lyapunov exponents of electrocardiogram (ECG) beats. The methodology consists of preprocessing, feature extraction and classification integrated into the system. PVC beats can be classified and differentiated from other types of abnormal heartbeats by analyzing Lyapunov exponents and training a learning vector quantization (LVQ) neural network. Our algorithm can obtain a good diagnostic result with little features by using single lead ECG data. The sensitivity, positive predictability, and the overall accuracy of the automatic diagnosis of PVC is 90.26%, 92.31%, and 98.90%, respectively. The effectiveness of the new method is validated through extensive tests using data from MIT-BIH database. The experimental results show that the proposed method is efficient and robust.
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http://dx.doi.org/10.1016/j.cmpb.2015.06.010DOI Listing
October 2015

Prevalence of hydatid cysts in livestock animals in Xinjiang, China.

Korean J Parasitol 2014 Jun 26;52(3):331-4. Epub 2014 Jun 26.

College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China.

Hydatid worms, hosted by humans and animals, impose serious human health risk and cause significant livestock production loss. To better understand the disease infection status in Xinjiang, China, we investigated the disease epidemics in 4 livestock animals, i.e., cattle, sheep (both sheep and goat), camels, and horses, slaughtered at the abattoirs in Urumqi, Yining, Tacheng, and Altay areas. The results showed that the animals were infected at different rates, in the order of sheep (9.8%), cattle (8.4%), camels (6.8%), and horses (4.3%). The infection rates were found to be different between the abattoirs in various regions even for the same animals. For sheep, the rates increased significantly as the animals grew older. It was 1.9% before 1 year of age and increased to 8.2% in the age of 1-2 years, and further increased to 12.3% when the animals were 3-4 years old, and reached 17.2% when they were 5-6 year old. Sheep older than 6 years had an infection rate of 19.5%. This study demonstrates that the 4 livestock animals in the pastoral areas in Xinjiang were infected by the parasites to various extend. This study is the first systematic investigation of the hydatid worms in various livestock animals in Xinjiang, China, which provides epidemiological information about the infection of hydatid worms in livestock, and is valuable in developing strategies for prevention and control of the hydatid disease.
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http://dx.doi.org/10.3347/kjp.2014.52.3.331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4096649PMC
June 2014

Nonlinear dynamics and quantum entanglement in optomechanical systems.

Phys Rev Lett 2014 Mar 18;112(11):110406. Epub 2014 Mar 18.

Institute for Complex Systems and Mathematical Biology, King's College, University of Aberdeen, Aberdeen AB24 3UE, United Kingdom.

To search for and exploit quantum manifestations of classical nonlinear dynamics is one of the most fundamental problems in physics. Using optomechanical systems as a paradigm, we address this problem from the perspective of quantum entanglement. We uncover strong fingerprints in the quantum entanglement of two common types of classical nonlinear dynamical behaviors: periodic oscillations and quasiperiodic motion. There is a transition from the former to the latter as an experimentally adjustable parameter is changed through a critical value. Accompanying this process, except for a small region about the critical value, the degree of quantum entanglement shows a trend of continuous increase. The time evolution of the entanglement measure, e.g., logarithmic negativity, exhibits a strong dependence on the nature of classical nonlinear dynamics, constituting its signature.
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http://dx.doi.org/10.1103/PhysRevLett.112.110406DOI Listing
March 2014

Effects of selective phosphodiesterases-4 inhibitors on learning and memory: a review of recent research.

Cell Biochem Biophys 2014 Sep;70(1):83-5

Department of Anesthesiology, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.

Phosphodiesterase-4 (PDE-4) regulates the intracellular level of cyclic adenosine monophosphate. Recent studies demonstrated that PDE-4 inhibitors can counteract deficits in long-term memory caused by aging or increased expression of mutant forms of human amyloid precursor proteins, and can influence the process of memory function and cognitive enhancement. Therapeutics, such as ketamine, a drug used in clinical anesthesia, can also cause memory deficits as adverse effects. Targeting PDE-4 with selective inhibitors may offer a novel therapeutic strategy to prevent, slow the progress, and, eventually, treat memory deficits.
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http://dx.doi.org/10.1007/s12013-014-9930-7DOI Listing
September 2014

RsbV of Listeria monocytogenes contributes to regulation of environmental stress and virulence.

Arch Microbiol 2013 Feb 29;195(2):113-20. Epub 2012 Nov 29.

College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China.

SigmaB factor is an important regulatory factor for stress response in Gram-positive bacteria such as Listeria monocytogenes (L. monocytogenes), Staphylococcus aureus and Bacillus subtilis. However, the activity of SigmaB factor is regulated by RsbV factor. Currently, the functional studies of RsbV factor are mostly focused on non-pathogenic B. subtilis, but the roles of RsbV factor in pathogenic L. monocytogenes during the regulation of environmental stress and virulence are still unclear. In the study, a ∆RsbV mutant of L. monocytogenes was constructed to explore the regulatory role of RsbV in environmental stress and virulence. The environmental stress experiments indicated that the growth and survival capability of ∆RsbV mutant obviously decreased in stress of low temperature, osmotic pressure, alcohol and acid, compared with EGD strain. The macrophage infection experiment indicated that ∆RsbV mutant had weaker survival capability than EGD strain, and the expression of PrfA, actA, PlcA and LLO was down-regulated in infected cells. Animal inoculation experiments indicated that RsbV deletion significantly reduced the pathogenicity of L. monocytogenes. Our data demonstrate that, in addition to regulating tolerance under environmental stress conditions, RsbV also contributes to regulation of L. monocytogenes virulence.
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http://dx.doi.org/10.1007/s00203-012-0855-5DOI Listing
February 2013

Effects of infusion of different fluids during controlled hypotension on gastric intramucosal pH and postoperative gastroenterological function.

J Biomed Res 2011 May;25(3):191-6

Department of Anesthesiology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.

The present study was aimed to investigate the effects of infusion of different fluids combined with controlled hypotension on gastric intramucosal pH (pHi) and postoperative gastrointestinal function in patients undergoing hepatocarcinoma surgery. Forty-five patients (ASA II) scheduled for surgical resection of hepatocarcinoma undergoing controlled hypotension were randomly assigned to three groups and received infusion of 20 mL/kg Ringer's solution (R group), 6% HAES(H group) or 6% Voluven group (W group). Intragastric PgCO2, pHi, hematocrit and hemoglobin were measured. The significant decrease of pHi and increase of PgCO2 were produced at 1 and 2 h after controlled hypotension in the R group (P < 0.05 or P < 0.01). The time of bowel movement after operation was shorter in the W group than the R group. Meanwhile, we also did not find obvious difference in blood gas indexes among the three groups. The infusion of HAES and Voluven during controlled hypotension could improve gastrointestinal perfusion and accelerate the recovery of postoperative gastrointestinal function.
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http://dx.doi.org/10.1016/S1674-8301(11)60025-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597062PMC
May 2011
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