Publications by authors named "Guanghui Wang"

383 Publications

Copper(I)-catalyzed tandem synthesis of 2-acylquinolines from 2-ethynylanilines and glyoxals.

Chem Commun (Camb) 2021 Oct 25. Epub 2021 Oct 25.

School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.

An efficient one-step synthesis of 2-acylquinolines using a copper-catalyzed tandem reaction of 2-ethynylanilines with glyoxals in the presence of piperidine has been developed. This new protocol successfully avoids multi-step operation and the use of highly toxic cyanides required in traditional methods, and provides a practical tool for synthetic and pharmaceutical chemists. Various 2-acylquinolines are obtained with perfect regioselectivity in moderate to good yields (up to 86%). The potential synthetic utility of this method is exemplified by a large-scale experiment and synthetic transformation of the products.
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http://dx.doi.org/10.1039/d1cc05612cDOI Listing
October 2021

Controllable refractive index sensing and multi-functional detecting based on the spin Hall effect of light.

Opt Express 2021 Aug;29(18):29481-29491

In this work, based on the spin Hall effect of light (SHEL), by considering the surface plasmon resonance (SPR) effect, a multi-functional detecting and controllable refractive index (RI) sensing structure containing sodium is theoretically established. The results reveal that the sodium layer has great influence on transverse shift (TS) of SHEL, while the polymethyl methacrylate (PMMA) layer has a large impact on the resonance angle. In the symmetrical distribution of TS, sodium has obvious advantages over gold and silver in the TS and sensitivity. The quantitative relationship between the TS and the RI of the sensing medium is established. Fermi energy, thicknesses of PMMA and sodium can be adjusted to measure the RI of three different orders. Remarkably, the sensitivity can be controlled by changing the thickness of sodium. The structure can also be used to measure the resonance angle and Fermi energy. Therefore, besides the advantages of sodium, this work realizes controllable sensing of RI and the functions of resonance angle and Fermi energy detecting. These studies may open avenues for the application of optical RI sensors and the precision measurement of other physical quantities.
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http://dx.doi.org/10.1364/OE.435775DOI Listing
August 2021

MicroRNA-137-mediated inhibition of lysine-specific demethylase-1 prevents against rheumatoid arthritis in an association with the REST/mTOR axis.

Mol Pain 2021 Jan-Dec;17:17448069211041847

Department of Orthopaedics Oncology, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Shandong, P.R. China.

Background: It has been increasingly reported that microRNAs (miRNAs) are related to rheumatoid arthritis (RA) pathogenesis. This present research was conducted to analyze the functions of miR-137 and the underlying molecular mechanism in RA progression.

Methods: Differentially expressed miRNAs in RA patients were analyzed using microarray-based analyses. Next, experiments involving miR-137 overexpression were performed to analyze the role of miR-137 in human fibroblast-like synoviocytes-RA (HFLS-RA) using cell counting kit-8 (CCK-8) assay, EdU staining, Transwell assay and flow cytometry, respectively. The function of miR-137 in inflammation was determined using ELISA. The binding relationship between miR-137 and LSD1 was confirmed by dual-luciferase reporter gene assay and ChIP test. Besides, a rat model with RA was established for experiments.

Results: miR-137 was downregulated in RA tissues and cells, which was negatively correlated with inflammatory factors. Upregulated miR-137 suppressed growth, migration and invasion of HFLS-RA, but promoted apoptosis. Lysine-specific demethylase-1 (LSD1) was a target of miR-137 and could be negatively regulated by miR-137. Moreover, LSD1 could activate REST through demethylation, while the REST/mTOR pathway induced levels of pro-inflammatory factors in RA. We observed the similar results in our study.

Conclusion: This study suggested that miR-137 reduced LSD1 expression to inhibit the activation of REST/mTOR pathway, thus preventing against inflammation and ameliorating RA development. Our research may offer new insights into treatment of RA.
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http://dx.doi.org/10.1177/17448069211041847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404669PMC
August 2021

Colonoscopy polyp detection and classification: Dataset creation and comparative evaluations.

PLoS One 2021 17;16(8):e0255809. Epub 2021 Aug 17.

Department of Computer Science, Ryerson University, Toronto, ON, Canada.

Colorectal cancer (CRC) is one of the most common types of cancer with a high mortality rate. Colonoscopy is the preferred procedure for CRC screening and has proven to be effective in reducing CRC mortality. Thus, a reliable computer-aided polyp detection and classification system can significantly increase the effectiveness of colonoscopy. In this paper, we create an endoscopic dataset collected from various sources and annotate the ground truth of polyp location and classification results with the help of experienced gastroenterologists. The dataset can serve as a benchmark platform to train and evaluate the machine learning models for polyp classification. We have also compared the performance of eight state-of-the-art deep learning-based object detection models. The results demonstrate that deep CNN models are promising in CRC screening. This work can serve as a baseline for future research in polyp detection and classification.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255809PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370621PMC
August 2021

Enhanced U-Net: A Feature Enhancement Network for Polyp Segmentation.

Proc Int Robot Vis Conf 2021 May 5;2021:181-188. Epub 2021 Jul 5.

Department of Computer Science, Ryerson University, Toronto ON, Canada, M5B 2K3.

Colonoscopy is a procedure to detect colorectal polyps which are the primary cause for developing colorectal cancer. However, polyp segmentation is a challenging task due to the diverse shape, size, color, and texture of polyps, shuttle difference between polyp and its background, as well as low contrast of the colonoscopic images. To address these challenges, we propose a feature enhancement network for accurate polyp segmentation in colonoscopy images. Specifically, the proposed network enhances the semantic information using the novel Semantic Feature Enhance Module (SFEM). Furthermore, instead of directly adding encoder features to the respective decoder layer, we introduce an Adaptive Global Context Module (AGCM), which focuses only on the encoder's significant and hard fine-grained features. The integration of these two modules improves the quality of features layer by layer, which in turn enhances the final feature representation. The proposed approach is evaluated on five colonoscopy datasets and demonstrates superior performance compared to other state-of-the-art models.
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http://dx.doi.org/10.1109/crv52889.2021.00032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341462PMC
May 2021

Impact of supervised beego, a traditional Chinese water-only fasting, on thrombosis and haemostasis.

BMJ Nutr Prev Health 2021 5;4(1):4-17. Epub 2021 Jan 5.

Hematology Center of Cyrus Tang Medical Institute, Soochow University, Suzhou, China.

Beego is a traditional Chinese complete water-only fasting practice initially developed for spiritual purposes, later extending to physical fitness purposes. Beego notably includes a psychological induction component that includes meditation and abdominal breathing, light body exercise and ends with a specific gradual refeeding program before returning to a normal diet. Beego has regained its popularity in recent decades in China as a strategy for helping people in subhealthy conditions or with metabolic syndrome, but we are unaware of any studies examining the biological effects of this practice. To address this, we here performed a longitudinal study of beego comprising fasting (7 and 14 day cohorts) and a 7-day programmed refeeding phase. In addition to detecting improvements in cardiovascular physiology and selective reduction of blood pressure in hypertensive subjects, we observed that beego decreased blood triacylglycerol (TG) selectively in TG-high subjects and increased cholesterol in all subjects during fasting; however, the cholesterol levels were normalised after completion of the refeeding program. Strikingly, beego reduced platelet formation, activation, aggregation and degranulation, resulting in an alleviated thrombosis risk, yet maintained haemostasis by sustaining levels of coagulation factors and other haemostatic proteins. Mechanistically, we speculate that downregulation of G6B and MYL9 may influence the observed beego-mediated reduction in platelets. Fundamentally, our study supports that supervised beego reduces thrombosis risk without compromising haemostasis capacity. Moreover, our results support that beego under medical supervision can be implemented as non-invasive intervention for reducing thrombosis risk, and suggest several lines of intriguing inquiry for future studies about this fasting practice (http://www.chictr.org.cn/index.aspx, number, ChiCTR1900027451).
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http://dx.doi.org/10.1136/bmjnph-2020-000183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258074PMC
January 2021

DJ-1 inhibits microglial activation and protects dopaminergic neurons in vitro and in vivo through interacting with microglial p65.

Cell Death Dis 2021 07 17;12(8):715. Epub 2021 Jul 17.

Jiangsu Key Laboratory of Translational Research and Therapy for Neuropsychiatric disorders & Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, 199 Ren'ai Road, Suzhou, Jiangsu, 215123, China.

Parkinson's disease (PD), one of the most common neurodegenerative disorders, is characterized by progressive neurodegeneration of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc). DJ-1 acts essential roles in neuronal protection and anti-neuroinflammatory response, and its loss of function is tightly associated with a familial recessive form of PD. However, the molecular mechanism of DJ-1 involved in neuroinflammation is largely unclear. Here, we found that wild-type DJ-1, rather than the pathogenic L166P mutant DJ-1, directly binds to the subunit p65 of nuclear factor-κB (NF-κB) in the cytoplasm, and loss of DJ-1 promotes p65 nuclear translocation by facilitating the dissociation between p65 and NF-κB inhibitor α (IκBα). DJ-1 knockout (DJ-1) mice exhibit more microglial activation compared with wild-type littermate controls, especially in response to lipopolysaccharide (LPS) treatment. In cellular models, knockdown of DJ-1 significantly upregulates the gene expression and increases the release of LPS-treated inflammatory cytokines in primary microglia and BV2 cells. Furthermore, DJ-1 deficiency in microglia significantly enhances the neuronal toxicity in response to LPS stimulus. In addition, pharmacological blockage of NF-κB nuclear translocation by SN-50 prevents microglial activation and alleviates the damage of DA neurons induced by microglial DJ-1 deficiency in vivo and in vitro. Thus, our data illustrate a novel mechanism by which DJ-1 facilitates the interaction between IκBα and p65 by binding to p65 in microglia, and thus repressing microglial activation and exhibiting the protection of DA neurons from neuroinflammation-mediated injury in PD.
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http://dx.doi.org/10.1038/s41419-021-04002-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286256PMC
July 2021

Spin Hall effect of Laguerre-Gaussian beams in PT symmetric metamaterials.

Opt Express 2021 Jul;29(14):22192-22201

Spin Hall effect (SHE) of Laguerre-Gaussian (LG) beams reflected and transmitted in parity-time (PT) symmetric metamaterials are investigated near the coherent-perfect-absorption (CPA)-laser point and exceptional points (EPs). The numerical results show that large transverse shifts occur at the CPA-laser point regardless of the incident direction. But at EPs, the SHE increases at one side and disappears at the other side, thus achieving the intense SHE of the reflected light beams at the specified side incidence. In addition, it is found that Bragg oscillation can be generated by increasing the period number of PT symmetric metamaterial layers, thus increasing the number of formants in transverse displacement. In particular, the transverse shift peaks of the transmitted beams merge into a positive peak when the incident angle is close to 90 and does not change basically with the increasing of Im(ɛ), which can also be considered as a strong tolerance to the variation of Im(ɛ). This feature is expected to realize a new type of optoelectronic devices with anti-interference performance. These results provide a feasible path for the modulation of spin Hall effect of light (SHEL) and provide the possibility for the development of new nanophotonic devices.
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http://dx.doi.org/10.1364/OE.427869DOI Listing
July 2021

Using protection motivation theory to explain the intention to initiate human papillomavirus vaccination among men who have sex with men in China.

Tumour Virus Res 2021 Jun 25;12:200222. Epub 2021 Jun 25.

School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, PR China; School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China; Kirby Institute, University of New South Wales, Sydney, Australia. Electronic address:

Background: Human papillomavirus (HPV) infection and related diseases are common among men who have sex with men (MSM). The most effective prevention is HPV vaccination. In China, however, men are not included in the HPV vaccination plan. We investigated the intention to initiate HPV vaccination and associated factors among MSM in China. Methods We surveyed 563 unvaccinated MSM aged 18 or older from six cities in China. Participants completed an electronic questionnaire about demographics, knowledge of and attitude towards HPV and HPV vaccine, intention to initiate HPV vaccination, willingness to recommend HPV vaccine to peers, feeling about government policy about HPV vaccination. We used the structural equation modeling (SEM) to analyze factors associated with HPV vaccine intention. Results The knowledge of HPV and HPV vaccine among participants was low. The mean score of knowledge about HPV and HPV vaccine was only 1.59 (range 0-11). The intention to initiate HPV vaccination within 6 months among participants was moderate (43.3% in total, 18.1% for 'very high' and 25.2% for 'above average').
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http://dx.doi.org/10.1016/j.tvr.2021.200222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261654PMC
June 2021

The Cross-Links of Endoplasmic Reticulum Stress, Autophagy, and Neurodegeneration in Parkinson's Disease.

Front Aging Neurosci 2021 3;13:691881. Epub 2021 Jun 3.

Department of Neurology, Center of Translational Medicine, Taicang Affiliated Hospital of Soochow University, The First People's Hospital of Taicang, Suzhou, China.

Parkinson's disease (PD) is the most common neurodegenerative movement disorder, and it is characterized by the selective loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc), as well as the presence of intracellular inclusions with α-synuclein as the main component in surviving DA neurons. Emerging evidence suggests that the imbalance of proteostasis is a key pathogenic factor for PD. Endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR) and autophagy, two major pathways for maintaining proteostasis, play important roles in PD pathology and are considered as attractive therapeutic targets for PD treatment. However, although ER stress/UPR and autophagy appear to be independent cellular processes, they are closely related to each other. In this review, we focused on the roles and molecular cross-links between ER stress/UPR and autophagy in PD pathology. We systematically reviewed and summarized the most recent advances in regulation of ER stress/UPR and autophagy, and their cross-linking mechanisms. We also reviewed and discussed the mechanisms of the coexisting ER stress/UPR activation and dysregulated autophagy in the lesion regions of PD patients, and the underlying roles and molecular crosslinks between ER stress/UPR activation and the dysregulated autophagy in DA neurodegeneration induced by PD-associated genetic factors and PD-related neurotoxins. Finally, we indicate that the combined regulation of ER stress/UPR and autophagy would be a more effective treatment for PD rather than regulating one of these conditions alone.
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http://dx.doi.org/10.3389/fnagi.2021.691881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218021PMC
June 2021

Enhanced phytoremediation of uranium-contaminated soils by Indian mustard (Brassica juncea L.) using slow release citric acid.

Environ Sci Pollut Res Int 2021 Jun 24. Epub 2021 Jun 24.

School of Resources and Environmental Science, Wuhan University, Wuhan, 430079, China.

In this study, a novel slow release carrier for the controlled release of citric acid (CA), hydroxypropyl chitosan-graft-carboxymethyl-β-cyclodextrin (HPCS-g-CMCD) was synthesized by the grafting reaction of carboxymethyl-β-cyclodextrin (CMCD) with hydroxypropyl chitosan (HPCS), and the structural characteristics of HPCS-g-CMCD were confirmed by FT-IR, TGA, and NMR. Based on HPCS-g-CMCD and CA, slow release citric acid (SRCA) was prepared by a spray drying method. HPCS-g-CMCD carrier has a better slow release performance for CA compared to HPCS and CMCD, and CA release mechanism was attributed to a Fickian diffusion. Furthermore, the release behavior of uranium in contaminated soil could be effectively controlled by SRCA. The effects of SRCA on improving the phytoremediation capacity in uranium-contaminated soil were investigated using Brassica juncea, which were grown in pots containing soil with uranium at 56 mg kg. After 50 days of growth, 5 mmol kg of CA, SRCA I, SRCA II, and SRCA III was applied, respectively. The results showed that slow release citric acid could enhance the uptake of uranium in Indian mustard. Uranium concentration in the root with SRCA I treatment was increased by 80.25% compared to the control, and the uranium removal efficiency of the SRCA I treatment was 1.66-fold greater than that of the control. Simultaneously, the leaching loss of uranium in SRCA I-treated soil was decreased by 37.35% compared to CA-treated soil. As a promising remediation strategy, SRCA-assisted phytoremediation may provide a kind of feasible technology with low leaching risk for remediation of uranium-contaminated soils.
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http://dx.doi.org/10.1007/s11356-021-14964-6DOI Listing
June 2021

IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells.

Cancer Cell Int 2021 Jun 3;21(1):290. Epub 2021 Jun 3.

Central Laboratory, The First People's Hospital of Taicang, Taicang Affiliated Hospital of Soochow University, No. 58 Changsheng South Road, Taicang, 215400, Jiangsu, China.

Background: Interferon regulatory factor-1 (IRF1) affects the proliferation of colorectal cancer (CRC). Recombinant interferon inducible protein 35 (IFI35) participates in immune regulation and cell proliferation. The aim of the study was to examine whether IRF1 affects the radiation sensitivity of CRC by regulating IFI35.

Methods: CCL244 and SW480 cells were divided into five groups: blank control, IFI35 upregulation, IFI35 upregulation control, IFI35 downregulation, and IFI35 downregulation control. All groups were treated with X-rays (6 Gy). IFI35 activation by IRF1 was detected by luciferase reporter assay. The GEPIA database was used to examine IRF1 and IFI35 in CRC. The cells were characterized using CCK-8, EdU, cell cycle, clone formation, flow cytometry, reactive oxygen species (ROS), and mitochondrial membrane potential. Nude mouse animal models were used to detect the effect of IFI35 on CRC.

Results: IRF1 can bind to the IFI35 promoter and promote the expression of IFI35. The expression consistency of IRF1 and IFI35 in CRC, according to GEPIA (R = 0.68, p < 0.0001). After irradiation, the upregulation of IFI35 inhibited cell proliferation and colony formation and promoted apoptosis and ROS, while IFI35 downregulation promoted proliferation and colony formation and reduced apoptosis, ROS, and mitochondrial membrane potential were also reduced. The in vivo experiments supported the in vitro ones, with smaller tumors and fewer liver metastases with IFI35 upregulation.

Conclusions: IRF1 can promote IFI35 expression in CRC cells. IFI35 is involved in the regulation of radiosensitivity of CRC cells and might be a target for CRC radiosensitization.
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http://dx.doi.org/10.1186/s12935-021-01997-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176734PMC
June 2021

Symmetric Pearcey Gaussian beams.

Opt Lett 2021 May;46(10):2461-2464

In this Letter, a new, to the best of our knowledge, type of autofocusing and symmetric beam arisen from two quartic spectral phases is introduced in theory and experiment. The symmetric Pearcey Gaussian beam (SPGB), formed with a Gaussian term and two multiplying Pearcey integrals, processes a focusing intensity approximately 1.32 times stronger than the intensity of the symmetric Airy beam. Its four off-axis main lobes split into four bending trajectories symmetrically after focusing. The rectangular intensity distribution and the focal length of the SPGB can be adjusted by two kinds of distribution factors. Additionally, the vortex-guiding property of the beam is demonstrated by embedding an off-axis vortex into the SPGB, which can be applied in particle guiding.
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http://dx.doi.org/10.1364/OL.425889DOI Listing
May 2021

Acceptability of male circumcision for HIV prevention among men who have sex with men in China: a short report.

AIDS Care 2021 Apr 28:1-8. Epub 2021 Apr 28.

School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, People's Republic of China.

Recent evidence shows that circumcision is associated with lower HIV prevalence among MSM. We assessed the acceptability of circumcision for preventing HIV and that of Shang Ring circumcision (SRC) among men who have sex with men (MSM) in China. 538 adult MSM were recruited from six cities in China between January and March 2019. Participants were surveyed by an online, self-administered questionnaire. The acceptability of circumcision was assessed before and after the potential protective effect of circumcision against HIV was informed, and subsequently men's willingness to undergo SRC was assessed. The level of circumcision was 16.4%. Of 450 uncircumcised MSM, their willingness to be circumcised in the following six months increased significantly from 32.2% to 55.6% after the information session. Three quarters of men who were willing to undergo circumcision accepted SRC. MSM who perceived that circumcision could maintain genital hygiene were more likely to accept circumcision after the information session, whereas those who regarded circumcision as an embarrassing surgery were disinclined to be circumcised. The low circumcision rate, along with its high acceptability in Chinese MSM, suggests a great potential benefit of circumcision intervention if proved effective. SRC might be a popular circumcision procedure in this population.
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http://dx.doi.org/10.1080/09540121.2021.1917759DOI Listing
April 2021

Assessing the potential for deep learning and computer vision to identify bumble bee species from images.

Sci Rep 2021 04 7;11(1):7580. Epub 2021 Apr 7.

Department of Electrical Engineering and Computer Science, University of Kansas, Lawrence, KS, USA.

Pollinators are undergoing a global decline. Although vital to pollinator conservation and ecological research, species-level identification is expensive, time consuming, and requires specialized taxonomic training. However, deep learning and computer vision are providing ways to open this methodological bottleneck through automated identification from images. Focusing on bumble bees, we compare four convolutional neural network classification models to evaluate prediction speed, accuracy, and the potential of this technology for automated bee identification. We gathered over 89,000 images of bumble bees, representing 36 species in North America, to train the ResNet, Wide ResNet, InceptionV3, and MnasNet models. Among these models, InceptionV3 presented a good balance of accuracy (91.6%) and average speed (3.34 ms). Species-level error rates were generally smaller for species represented by more training images. However, error rates also depended on the level of morphological variability among individuals within a species and similarity to other species. Continued development of this technology for automatic species identification and monitoring has the potential to be transformative for the fields of ecology and conservation. To this end, we present BeeMachine, a web application that allows anyone to use our classification model to identify bumble bees in their own images.
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http://dx.doi.org/10.1038/s41598-021-87210-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027374PMC
April 2021

Optical conveyor belt based on a plasmonic metasurface with polarization dependent hot spot arrays.

Opt Lett 2021 Apr;46(7):1522-1525

In this Letter, we propose a novel metasurface conveyor belt with periodic orientated arrays of gold plasmonic elliptical elements (GPEEs), which can be continuously lit in a relay way by switching the polarization of the excitation beam and can be used to trap, transport, and sort particles. The array of the hot field can provide a larger trapping area and better stiffness. With the incident optical intensity of 0.08/µ, the depth of the potential well could be as high as 10. By setting a narrow interval between plasmonic ellipses in principal axes, it can help further enhance their directional resonant coupling and polarization dependence. Furthermore, based on consideration of the Brownian motion of trapped particles in aqueous solution, we analyzed its time response property of particle manipulation with different applied switching frequencies from a statistical point of view. As confirmed by numerical analysis, our design offers a novel scheme of particle sorting using a scalable hot spot array with better performance, which could be used in many on-chip optofluidic applications.
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http://dx.doi.org/10.1364/OL.419201DOI Listing
April 2021

miR-34a-5p regulates PINK1-mediated mitophagy via multiple modes.

Life Sci 2021 Jul 26;276:119415. Epub 2021 Mar 26.

Center for Drug Safety Evaluation and Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China; University of Chinese Academy of Sciences, Beijing, China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, China. Electronic address:

Aims: PTEN induced putative kinase 1 (PINK1)-mediated mitophagy process is tightly associated with various age-dependent diseases in mammals. The roles of miRNAs (miRNAs) in the PINK1-mediated mitophagy process are not fully understood. Here we discovered that miR-34a-5p suppresses PINK1 expression directly though two post-transcriptional non-classical binding modes, resulting in inhibition of PINK1-mediated mitophagy process.

Main Methods: For in vivo experiments, brains were dissected from 8 weeks old and 40 weeks old C57BL/6 male mice to measure miR-34a-5p expression and PINK1 expression. For in vitro experiments, overexpression of miR-34a-5p mimics in HEK293 cells was performed to investigate the effect of miR-34a-5p on PINK1 expression and its regulatory mechanism, parkin recruitment and mitophagy process.

Key Findings: The level of miR-34a-5p was upregulated and the level of PINK1 mRNA was downregulated in brains of aged mice. Both the 3'-untranslated region (3'UTR) and the Coding DNA sequence (CDS) of PINK1 mRNA were bound to the non-seed region of miR-34a-5p, rather than the seed region, resulting in a decrease in PINK1 expression. Endogenous miR-34a-5p knockout increased PINK1 expression. Further results indicated that miR-34a-5p inhibits mitophagy process by reduction of PINK1. miR-34a-5p hinders phosphorylated Ser65-ubiquitin (pS65-Ub) accumulation, prevents the mitochondrial recruitment of Parkin, attenuates ubiquitination and delays the clearance of damaged mitochondria.

Significance: We firstly found that miR-34a-5p suppresses PINK1 directly and further regulates mitophagy through non-canonical modes. This finding hints at a crucial role of miR-34a-5p implicated in accelerating the pathogenesis of age-related neurological diseases.
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http://dx.doi.org/10.1016/j.lfs.2021.119415DOI Listing
July 2021

Screening and verification of hub genes involved in osteoarthritis using bioinformatics.

Exp Ther Med 2021 Apr 8;21(4):330. Epub 2021 Feb 8.

Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Hand and Foot Surgery Department, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, Guangdong 518000, P.R. China.

Osteoarthritis (OA) is one of the most common causes of disability and its development is associated with numerous factors. A major challenge in the treatment of OA is the lack of early diagnosis. In the present study, a bioinformatics method was employed to filter key genes that may be responsible for the pathogenesis of OA. From the Gene Expression Omnibus database, the datasets GSE55457, GSE12021 and GSE55325 were downloaded, which comprised 59 samples. Of these, 30 samples were from patients diagnosed with osteoarthritis and 29 were normal. Differentially expressed genes (DEGs) were obtained by downloading and analyzing the original data using bioinformatics. The Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathways were analyzed using the Database for Annotation, Visualization and Integrated Discovery online database. Protein-protein interaction network analysis was performed using the Search Tool for the Retrieval of Interacting Genes/proteins online database. BSCL2 lipid droplet biogenesis associated, seipin, FOS-like 2, activator protein-1 transcription factor subunit (FOSL2), cyclin-dependent kinase inhibitor 1A (CDKN1A) and kinectin 1 (KTN1) genes were identified as key genes by using Cytoscape software. Functional enrichment revealed that the DEGs were mainly accumulated in the ErbB, MAPK and PI3K-Akt pathways. Reverse transcription-quantitative PCR analysis confirmed a significant reduction in the expression levels of FOSL2, CDKN1A and KTN1 in OA samples. These genes have the potential to become novel diagnostic and therapeutic targets for OA.
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http://dx.doi.org/10.3892/etm.2021.9761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903481PMC
April 2021

Phosphorus-modified biochar cross-linked Mg-Al layered double-hydroxide composite for immobilizing uranium in mining contaminated soil.

Chemosphere 2021 Aug 26;276:130116. Epub 2021 Feb 26.

School of Resources and Environmental Science, Wuhan University, Wuhan, 430079, China.

The decommissioning of uranium mill tailings (UMTs) is usually accompanied by uranium (U) contamination in soil, which poses a serious threat to human health and ecological safety. In this study, a novel phosphorus-modified bamboo biochar (PBC) cross-linked Mg-Al layered double-hydroxide (LDH) composite ("[email protected]") was successfully prepared by phosphate pre-impregnation and a hydrothermal method with Mg-Al LDH. Physicochemical analysis revealed that phosphorus-containing functional groups and Mg-Al LDH were grafted onto the pristine biochar (BC) matrix. Laboratory-scale incubation and column leaching experiments were performed on the prepared BC, PBC, and [email protected] The results showed that, at a dosage of 10%, the [email protected] composite had a commendable ability to immobilize U in soil. After 40 days of incubation with the stabilizer, the more mobile U was converted into immobilized species. Furthermore, during a column leaching experiment with simulated acid rain, the cumulative loss and leaching efficiency of U were remarkably reduced by [email protected] treatment compared with the control, reaching 53% and 54%, respectively. Surface complexation, co-precipitation, and reduction described the adsorption and immobilization mechanisms. In conclusion, this research demonstrates that the [email protected] composite offers a potentially effective amendment for the remediation of U contaminated soil.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130116DOI Listing
August 2021

FgBUD14 is important for ascosporogenesis and involves both stage-specific alternative splicing and RNA editing during sexual reproduction.

Environ Microbiol 2021 Sep 9;23(9):5052-5068. Epub 2021 Mar 9.

College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.

In wheat head blight fungus Fusarium graminearum, A-to-I RNA editing occurs specifically during sexual reproduction. Among the genes with premature stop codons (PSCs) that require RNA editing to encode full-length proteins, FgBUD14 also had alternative splicing events in perithecia. In this study, we characterized the functions of FgBUD14 and its post-transcriptional modifications during sexual reproduction. The Fgbud14 deletion mutant was slightly reduced in growth, conidiation and virulence. Although deletion of FgBUD14 had no effect on perithecium morphology, the Fgbud14 mutant was defective in crozier formation and ascus development. The FgBud14-GFP localized to the apex of ascogenous hyphae and croziers, which may be related to its functions during early sexual development. During vegetative growth and asexual reproduction, FgBud14-GFP localized to hyphal tips and both ends of conidia. Furthermore, mutations blocking the splicing of intron 2 that has the PSC site had no effect on the function of FgBUD14 during sexual reproduction but caused a similar defect in growth with Fgbud14 mutant. Expression of the non-editable FgBUD14 mutant allele also failed to complement the Fgbud14 mutant. Taken together, FgBUD14 plays important roles in ascus development, and both alternative splicing and RNA editing occur specifically to its transcripts during sexual reproduction in F. graminearum.
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http://dx.doi.org/10.1111/1462-2920.15446DOI Listing
September 2021

Nuclear TEAD4 with SIX1 Overexpression is an Independent Prognostic Marker in the Stage I-III Colorectal Cancer.

Cancer Manag Res 2021 17;13:1581-1589. Epub 2021 Feb 17.

Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Introduction: Stage I-III colorectal cancer patients are under risk of tumor recurrence and metachronous metastasis after radical surgery. An increased expression of transcription factor TEAD4 is associated with epithelial-mesenchymal transition, metastasis and poor prognosis in colorectal cancer. However, the mechanistic role of TEAD4 in driving colon cancer progression and its prognostic value in early stage of CRC remains unclear.

Methods: In this study, the regulation, function and prognostic significance of TEAD4 and its new direct target gene SIX1 in CRC progression were evaluated using human tissues, molecular and cell biology.

Results: We show that TEAD4 directly upregulates the expression of SIX1 at transcriptional level in CRC cells, establishing that SIX1 is a new direct target gene of TEAD4. TEAD4 promotes EMT and cell migration of CRC cells, while SIX1 knockdown attenuates this effect and SIX1 overexpression enhances this effect, indicating that SIX1 mediates the function of TEAD4 in promoting cell migration in CRC cells. Clinically, nuclear TEAD4, overexpression of SIX1 and nuclear TEAD4 with SIX1 overexpression predict poor prognosis in CRC patients.

Discussion: Our study identifies TEAD4-SIX1-CDH1 form a novel signaling axis, which contributes to CRC progression, and its aberrant expression and activation predicts poor prognostic for CRC patients in stage I-III.
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http://dx.doi.org/10.2147/CMAR.S260790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898202PMC
February 2021

Carbon Nanodots as a Multifunctional Fluorescent Sensing Platform for Ratiometric Determination of Vitamin B and "Turn-Off" Detection of pH.

J Agric Food Chem 2021 Mar 23;69(9):2836-2844. Epub 2021 Feb 23.

Institute of Environmental Science and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, Shanxi 030006, People's Republic of China.

In this work, we synthesized carbon nanodots (CNDs) by a one-pot hydrothermal method to carbonize precursors of dry carnation petals and polyethylenimine. The obtained CNDs possess favorable photostability, good biocompatibility, and excellent water solubility, which can serve as a dual-responsive nanosensor for the determination of vitamin B (VB) and pH. A unique ratiometric fluorescence resonance energy transfer probe was developed through a strong interaction between VB and surface moieties of CNDs. CNDs emitted at 470 nm; however, in the presence of VB, an enhanced emission peak was clearly observed at 532 nm. The value of / exhibits a stable response to the VB concentration from 0.35 to 35.9 μM with a detection limit of 37.2 nM, which has been used for VB detection in food and medicine samples and ratiometric imaging of VB in living cells with satisfying performance. In addition, the proposed CNDs also displayed pH-sensitive behavior and can be a turn-off fluorescent sensor to monitor pH. The fluorescent intensity at 470 nm is a good linear response against pH values from 3.6 to 8, affording the capability as a single-emissive nanoprobe for intracellular pH sensing.
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http://dx.doi.org/10.1021/acs.jafc.0c07019DOI Listing
March 2021

Long noncoding RNA CRNDE functions as a diagnostic and prognostic biomarker in osteosarcoma, as well as promotes its progression via inhibition of miR-335-3p.

J Biochem Mol Toxicol 2021 May 1;35(5):e22734. Epub 2021 Feb 1.

Department of Orthopedic Surgery, Zhumadian Central Hospital, Zhumadian, China.

Background: This study was performed to evaluate the diagnostic and prognostic value, as well as the role of long-chain noncoding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) in osteosarcoma (OS).

Materials And Methods: A quantitative real-time polymerase chain reaction assay was to determine lncRNA CRNDE and microRNA-335-3p (miR-335-3p) expressions. The Kaplan-Meier analysis was to analyze the relationship between lncRNA CRNDE expression and survival in patients with OS. Receiver operating characteristic curves were to evaluate the diagnostic value of lncRNA CRNDE in OS. Bioinformatics analysis and luciferase reporter assays were used to predict and confirm the relationship between lncRNA CRNDE and miR-335-3p. Cell counting Kit-8 and transwell migration assays assessed the role of lncRNA CRNDE and miR-335-3p in OS cells.

Results: lncRNA CRNDE expression was upregulated and miR-355-3p expression was downregulated in OS. In patients with OS, low lncRNA CRNDE expression demonstrated higher overall survival, whereas high lncRNA CRNDE expression was an independent poor prognostic factor. Furthermore, increased lncRNA CRNDE expression was associated with distant metastasis and the tumor-node-metastasis stage in patients with OS, which can be considered as an independent diagnostic biomarker in OS. We revealed that miR-335-3p was the target of lncRNA CRNDE. It also demonstrated that knockdown of lncRNA CRNDE inhibited OS cell proliferation, migration, and invasion, and inhibition of miR-355-3p promoted this effect. Finally, miR-335-3p partially mediated the stimulatory effects of lncRNA CRNDE in OS.

Conclusion: We demonstrated that lncRNA CRNDE is a potential diagnostic and prognostic biomarker for OS, and the lncRNA CRNDE/miR-335-3p axis participates in OS progression.
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http://dx.doi.org/10.1002/jbt.22734DOI Listing
May 2021

Long noncoding RNA CRNDE functions as a diagnostic and prognostic biomarker in osteosarcoma, as well as promotes its progression via inhibition of miR-335-3p.

J Biochem Mol Toxicol 2021 May 1;35(5):e22734. Epub 2021 Feb 1.

Department of Orthopedic Surgery, Zhumadian Central Hospital, Zhumadian, China.

Background: This study was performed to evaluate the diagnostic and prognostic value, as well as the role of long-chain noncoding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) in osteosarcoma (OS).

Materials And Methods: A quantitative real-time polymerase chain reaction assay was to determine lncRNA CRNDE and microRNA-335-3p (miR-335-3p) expressions. The Kaplan-Meier analysis was to analyze the relationship between lncRNA CRNDE expression and survival in patients with OS. Receiver operating characteristic curves were to evaluate the diagnostic value of lncRNA CRNDE in OS. Bioinformatics analysis and luciferase reporter assays were used to predict and confirm the relationship between lncRNA CRNDE and miR-335-3p. Cell counting Kit-8 and transwell migration assays assessed the role of lncRNA CRNDE and miR-335-3p in OS cells.

Results: lncRNA CRNDE expression was upregulated and miR-355-3p expression was downregulated in OS. In patients with OS, low lncRNA CRNDE expression demonstrated higher overall survival, whereas high lncRNA CRNDE expression was an independent poor prognostic factor. Furthermore, increased lncRNA CRNDE expression was associated with distant metastasis and the tumor-node-metastasis stage in patients with OS, which can be considered as an independent diagnostic biomarker in OS. We revealed that miR-335-3p was the target of lncRNA CRNDE. It also demonstrated that knockdown of lncRNA CRNDE inhibited OS cell proliferation, migration, and invasion, and inhibition of miR-355-3p promoted this effect. Finally, miR-335-3p partially mediated the stimulatory effects of lncRNA CRNDE in OS.

Conclusion: We demonstrated that lncRNA CRNDE is a potential diagnostic and prognostic biomarker for OS, and the lncRNA CRNDE/miR-335-3p axis participates in OS progression.
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http://dx.doi.org/10.1002/jbt.22734DOI Listing
May 2021

Selenium-Containing Amino Acids Protect Dextran Sulfate Sodium-Induced Colitis via Ameliorating Oxidative Stress and Intestinal Inflammation.

J Inflamm Res 2021 14;14:85-95. Epub 2021 Jan 14.

Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, People's Republic of China.

Background: Inflammatory bowel disease (IBD) is characterized by chronic relapsing inflammation of the gastrointestinal tract. Oxidative stress plays a pivotal role in the pathogenesis of IBD. Selenium-containing amino acids reportedly have anti-oxidative and anti-inflammatory properties, but it remains unknown if selenium-containing amino acids can be used to treat IBD. This study aimed to investigate the effects of two selenium-containing amino acids - selenocysteine and selenocystine - on oxidative stress and chronic inflammation in a mouse model of dextran sulfate sodium (DSS)-induced IBD.

Methodology: C57BL/6 mice were randomly assigned to the following six groups: control, DSS, DSS+selenocysteine, DSS+selenocystine, DSS+sodium selenite, and DSS+N-acetylcysteine (NAC). IBD was induced by 3% DSS. Pro-inflammatory cytokines [interleukin-1β (IL-1β), monocyte chemotactic protein 1 (MCP-1), IL-6, and tumor necrosis factor-α (TNF-α)] and markers for oxidative and anti-oxidative stress [malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione peroxidase (GPx)] were measured using immunohistochemical analysis.

Results: Selenocysteine and selenocystine significantly attenuated IBD-related symptoms, including preventing weight loss, decreasing disease activity index (DAI) scores, and increasing colon length. Selenocysteine and selenocystine significantly ameliorated the DSS-induced oxidative stress, as demonstrated by a reduction in ROS and MDA activity and an increase in SOD and GPx activity. IL-1, MCP-1, IL-6, and TNF-α levels were significantly increased in the IBD mice, while treatment with the selenium-containing amino acids significantly reduced the levels of these pro-inflammatory cytokines. In vivo safety analysis showed minimal side effects of the selenium-containing amino acids.

Conclusion: We found that selenocysteine and selenocystine ameliorated DSS-induced IBD via reducing oxidative stress and intestinal inflammation, indicating that selenium-containing amino acids could be a novel therapeutic option for patients with IBD.
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http://dx.doi.org/10.2147/JIR.S288412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814278PMC
January 2021

Jatrophane Diterpenoids from as Multidrug Resistance Modulators with Inhibitory Effects on the ATR-Chk-1 Pathway.

J Nat Prod 2021 02 14;84(2):339-351. Epub 2021 Jan 14.

Fujian Provincial Key Laboratory of Innovative Drug Target, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, People's Republic of China.

Twelve undescribed jatrophane diterpenoids, euphpepluones A-L (-), together with seven known analogues (-), were isolated from the whole plant of , and their structures were elucidated by spectroscopic studies. The absolute configurations of and were assigned by X-ray crystallographic analysis. All isolates were investigated for their inhibitory effects against the ATR-Chk1 pathway using a Western blotting assay. As a result, , , , , , and were found to suppress the camptothecin (CPT)-induced phosphorylation of Chk1, indicating that these compounds inhibit the activation of the ATR-Chk1 pathway. A preliminary structure-activity relationship (SAR) study of the isolates was conducted. When compound and CPT were combined, apoptosis was induced in A549 cells with PARP cleavage, while there was no apoptotic effect by treatment with CPT or alone. The data obtained indicate that potentiates the chemotherapeutic sensitivity of A549 cells to CPT.
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http://dx.doi.org/10.1021/acs.jnatprod.0c00986DOI Listing
February 2021

The CHY-Type Zinc Finger Protein FgChy1 Regulates Polarized Growth, Pathogenicity, and Microtubule Assembly in .

Mol Plant Microbe Interact 2021 Apr 10;34(4):362-375. Epub 2021 Mar 10.

Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China.

Microtubules (MTs), as transport tracks, play important roles in hyphal-tip growth in filamentous fungi, but MT-associated proteins involved in polarized growth remain unknown. Here, we found that one novel zinc finger protein, FgChy1, is required for MT morphology and polarized growth in . The mutant presented curved and directionless growth of hyphae. Importantly, the conidia and germ tubes of the mutant exhibited badly damaged and less-organized beta-tubulin cytoskeletons. Compared with the wild type, the mutant lost the ability to maintain polarity and was also more sensitive to the anti-MT drugs carbendazim and nocodazole, likely due to the impaired MT cytoskeleton. Indeed, the hyphae of the wild type treated with nocodazole exhibited a morphology consistent with that of the mutant. Interestingly, the disruption of FgChy1 resulted in the off-center localization of actin patches and the polarity-related polarisome protein FgSpa2 from the hyphal-tip axis. A similar defect in FgSpa2 localization was also observed in the nocodazole-treated wild-type strain. In addition, FgChy1 is also required for conidiogenesis, septation, sexual reproduction, pathogenicity, and deoxynivalenol production. Overall, this study provides the first demonstrations of the functions of the novel zinc finger protein FgChy1 in polarized growth, development, and virulence in filamentous fungi.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
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http://dx.doi.org/10.1094/MPMI-07-20-0206-RDOI Listing
April 2021

Knockdown of DDX46 suppresses the proliferation and invasion of gastric cancer through inactivating Akt/GSK-3β/β-catenin pathway.

Exp Cell Res 2021 02 19;399(1):112448. Epub 2020 Dec 19.

Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. Electronic address:

DEAD-box RNA helicase 46 (DDX46) has recently been identified as a candidate oncogene in several types of human malignancies. To date, the role of DDX46 in gastric cancer has not been determined. The purpose of the current study was to explore the role of DDX46 in gastric cancer and the potential mechanism. DDX46-silecing or overexpressing gastric cancer cell lines were established to validate the role of DDX46. Our results showed that the expression of DDX46 was significantly increased in gastric cancer tissues and cell lines. Knockdown of DDX46 suppressed the proliferation and invasion of gastric cancer cells. Whereas, DDX46 overexpression enhanced the cell proliferation and invasion of gastric cancer cells. Furthermore, knockdown of DDX46 markedly suppressed the tumor growth of xenografts. Research into the mechanism revealed that DDX46 depletion inhibited the Akt/GSK-3β/β-catenin signaling pathway in gastric cancer cells. Notably, activation of Akt or β-catenin overexpression reversed the DDX46 depletion-mediated anti-cancer effect. In conclusion, these findings indicated that DDX46 exerted an oncogenic role in gastric cancer via regulating the Akt/GSK-3β/β-catenin signaling pathway. Thus, DDX46 might be utilized as a therapeutic anti-cancer target.
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http://dx.doi.org/10.1016/j.yexcr.2020.112448DOI Listing
February 2021

Study of the co-expression gene modules of non-small cell lung cancer metastases.

Cancer Biomark 2021 ;30(3):321-329

Institute of Oncology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

Background: Metastasis regularly is a marker of the disease development of cancers. Some metastatic sites significantly showed more serious clinical outcomes in non-small cell lung cancer (NSCLC). Whether they are caused by tissue-specific (TS) or non-tissue-specific (NTS) mechanisms is still unclear.

Objective: Explore co-expression gene modules of non-small cell lung cancer metastases.

Methods: Weighted Correlation Network Analysis (WGCNA) was used to identify the gene modules among the metastases of NSCLC. The clinical significance of those gene modules was evaluated with the Cox hazard proportional model with another independent dataset. Functions of each gene module were analyzed with gene ontology. Typical genes were further studied.

Results: There were two TS gene modules and two NTS gene modules identified. One TS gene module (green module) and one NTS gene module (purple module) significantly correlated with survival. This NTS gene module (purple module) was significantly enriched in the epithelial-to-mesenchymal transition (EMT) process. Higher expression of the typical genes (CA14, SOX10, TWIST1, and ALX1) from EMT process was significantly associated with a worse survival.

Conclusion: The lethality of NSCLC metastases was caused by TS gene modules and NTS gene modules, among which the EMT-related gene module was critical for a worse clinical outcome.
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http://dx.doi.org/10.3233/CBM-201605DOI Listing
October 2021

A new phenolic glycoside from Bunge with anti-inflammatory and antibacterial activities.

Nat Prod Res 2020 Dec 8:1-8. Epub 2020 Dec 8.

Fujian Provincial Key Laboratory of Innovative Drug Target, School of Pharmaceutical Sciences, Xiamen University, Xiamen, People's Republic of China.

A undescribed phenolic glycoside, trochinenol A (), was isolated from the flowers of Bunge and the structure was identified by spectroscopic methods. Its anti-inflammatory and antibacterial effects were investigated by broth microdilution and NF-κB reporter gene assays. Consequently, compound exhibited an appreciable effect against with the MIC value of 6.25 µg/mL. Besides, it showed moderate effect against TNFα-induced activation of NF-κB pathway.
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http://dx.doi.org/10.1080/14786419.2020.1855166DOI Listing
December 2020
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