Publications by authors named "Guang-Hui Cao"

2 Publications

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BM-MSCs-derived microvesicles promote allogeneic kidney graft survival through enhancing micro-146a expression of dendritic cells.

Immunol Lett 2017 11 28;191:55-62. Epub 2017 Sep 28.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, China.

Objective: Microvesicles (MVs) are plasmalemmal vesicles that are released from various cells and regarded as a mediator of intermolecular communication. In present study, we aimed to evaluate the therapeutic efficacy of the bone marrow mesenchymal stem cells (BM-MSCs)-derived MVs in the mice kidney transplant model and explored the underlying mechanism.

Methods: BM-MSCs were isolated from C57BL/6 mice and identified using flow cytometry. In vivo allogenic kidney transplantation model of mice was performed between C57BL/6 mice (recipient) and BALB/c mice (donor). Recipient-type BM-MSC (0.1ml) or equal volume of medium as a control was injected i.v. 24h after kidney transplantation. Serum was collected for creatinine concentration detection at 14 d after transplantation. Dendritic cells (DCs) phenotype and miR-146a expression level in plant was identified. Immature DCs (iDCs) and mature DCs (mDCs) were derived from monocytes. MVs were separated from BM-MSCs.

Results: BM-MSCs positive for CD29 (95.8%) and CD44 (94.7%) were cultured and confirmed to prolong the allogenic kidney graft survival in mice. Importantly, the expression of miR-146a increased significantly in DCs of BM-MSCs-treated allogenic kidney. Moreover, both BM-MSCs and MVs derived from BM-MSCs enhanced miR-146a expression in iDCs and mDCs in vitro. Furthermore, MVs substantially reduced IL-12 mRNA expression and IL-12 production of mDCs whereas this action was reversed by miR-146a silencing. MiR-146a silencing also abrogated the MVs-induced decrease in serum creatinine, reduction of immature DCs phenotype in transplant and increase in miR-146a expression level.

Conclusion: In summary, our data suggested that the BM-MSCs-derived MVs improved allogenic kidney transplantation survival through inhibiting DCs maturity by miR-146a.
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http://dx.doi.org/10.1016/j.imlet.2017.09.010DOI Listing
November 2017

A study of the relationship between trace element Mo and gastric cancer.

World J Gastroenterol 1998 Feb;4(1):55-56

AIM:To study the relationship between trace element Mo and gastric cancer.MATERIALS AND METHODS: Soil samples were collected according to its type in different areas of Jiangxi Province; available molybdenum content in soil was measured by catalytic polarography and rank correlation method was used to analyse correlation between the mean of soil available molybdenum and mortality rate of gastric cancer in each county and city in Jiangxi Province. Gastric cancer cases were selected from the authors' hospital, occiput hair was collected to measure its molybdenum content with an atomic absorption spectrograph and controls were selected from the same hospital for comparison. Gastric cancer cases were selected from three hospitals at the same time, blood samples were taken on an empty stomach and serum molybdenum contents were measured with the atomic absorption spectrograph, and controls were selected from the same hospitals. Blind method was used in the whole course (chemical analysts did not know the source and nature of samples).RESULTS: A negative correlation existed between soil available molybdenum content and mortality rate of gastric cancer (r = -0.285, P < 0.05); hair molybdenum contents of gastric cancer cases were lower than those of healthy controls (0.308&mgr;g/g plus minus 0.673&mgr;g/g and 0.707&mgr;g/g plus minus 0.561&mgr;g/g respectively, P < 0.01); serum molybdenum contents of patients were also lower than those of healthy controls (21.84&mgr;g/L plus minus 7.49&mgr;g/L and 25.38&mgr;g/L plus minus 8.58&mgr;g/L respectively,P< 0.05).CONCLUSION: Deficiency of molybdenum may be one of the risk factors in gastric cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767765PMC
http://dx.doi.org/10.3748/wjg.v4.i1.55DOI Listing
February 1998
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