Publications by authors named "Guang Li"

799 Publications

Single-cell RNA sequencing of peripheral blood mononuclear cells from acute Kawasaki disease patients.

Nat Commun 2021 Sep 14;12(1):5444. Epub 2021 Sep 14.

Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai, China.

Kawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Although functional and phenotypic changes of immune cells have been reported, a global understanding of immune responses underlying acute KD is unclear. Here, using single-cell RNA sequencing, we profile peripheral blood mononuclear cells from seven patients with acute KD before and after intravenous immunoglobulin therapy and from three age-matched healthy controls. The most differentially expressed genes are identified in monocytes, with high expression of pro-inflammatory mediators, immunoglobulin receptors and low expression of MHC class II genes in acute KD. Single-cell RNA sequencing and flow cytometry analyses, of cells from an additional 16 KD patients, show that although the percentage of total B cells is substantially decreased after therapy, the percentage of plasma cells among the B cells is significantly increased. The percentage of CD8 T cells is decreased in acute KD, notably effector memory CD8 T cells compared with healthy controls. Oligoclonal expansions of both B cell receptors and T cell receptors are observed after therapy. We identify biological processes potentially underlying the changes of each cell type. The single-cell landscape of both innate and adaptive immune responses provides insights into pathogenesis and therapy of KD.
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http://dx.doi.org/10.1038/s41467-021-25771-5DOI Listing
September 2021

The effect of smoking status on efficacy of immune checkpoint inhibitors in metastatic non-small cell lung cancer: A systematic review and meta-analysis.

EClinicalMedicine 2021 Aug 26;38:100990. Epub 2021 Jun 26.

Department of Radiation Oncology, The First Hospital of China Medical University, 155 Nanjing Road, Heping District, Shenyang 110001, China.

Background: It remains uncertain whether smoking status can effect efficacy of immune checkpoint inhibitors (ICIs) in metastatic non-small cell lung cancer (NSCLC). We performed a meta-analysis to address this issue.

Patients And Methods: PubMed, Embase, Cochrane Library, Web of Science, and international meetings were searched until April 1, 2021, for phase 2 and 3 randomized controlled trials (RCTs) which compared ICIs with chemotherapy (CT) and reported overall survival (OS) and/or progression-free survival (PFS) data according to smoking status. This meta-analysis was registered in INPLASY platform (#INPLASY202140025). The random-effect model was used for statistical analysis.

Findings: Twenty-eight articles from 24 RCTs including 13918 patients were eligible. ICIs significantly prolonged OS than CT in smokers (hazard ratio [HR] = 0.75, 95% confidence interval [CI]: 0.69-0.81), but not in never-smokers (HR = 0.87, 95% CI: 0.74-1.04); while there was no significant treatment-smoking interaction (P = 0.11). Significant heterogeneity was observed for both smokers (OS: I = 60%, P = 0.0002; PFS: I = 74%, P < 0.0001) and never smokers (PFS: I = 69%, P < 0.0001). Subgroup analyses revealed that ICIs monotherapy significantly improved OS in smokers (HR = 0.76, 95% CI: 0.69-0.85) but not in never-smokers (HR = 0.93, 95% CI: 0.77-1.12, P = 0.07), and treatment-smoking interaction was significant in patients with PD-L1 ≥50% (HR, 0.61 vs 1.18; P = 0.005). ICIs plus CT achieved better OS either in smokers or never-smokers (HR, 0.76 vs 0.61; P = 0.39), while dual ICIs combination prolonged OS only in smokers but never-smokers (HR, 0.68 vs 1.02; P = 0.02).

Interpretation: Either ICIs monotherapy or combination therapy was superior to CT in smokers. While ICIs monotherapy and dual ICIs combination were less effective in never-smokers, and ICIs plus CT might be the optimal selection. Nevertheless, given the limitation of the high heterogeneity of studies included, the findings need to be confirmed by future RCTs focusing on this subject.

Funding: None.
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http://dx.doi.org/10.1016/j.eclinm.2021.100990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413256PMC
August 2021

Instant hydrogelation encapsulates drugs onto implants intraoperatively against osteoarticular tuberculosis.

J Mater Chem B 2021 Sep 7. Epub 2021 Sep 7.

CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.

Osteoarticular Tuberculosis (TB) is a challenging issue because of its chronicity and recurrence. Many drug delivery systems (DDSs) have been developed for general chemotherapy. Herein, we take advantage of instant hydrogelation to encapsulate drugs onto implants intraoperatively, optimizing the drug release profile against osteoarticular TB. First-line chemodrugs, rifampicin (RFP) and isoniazid (INH) are firstly loaded on tricalcium phosphate (TCP). Then, the encapsulating hydrogel is fabricated by dipping in chitosan (CS) and β-glycerophosphate (β-GP) solution and heating at 80 °C for 40 min. The hydrogel encapsulation inhibits explosive drug release initially, but maintains long-term drug release (INH, 158 days; RFP, 53 days) . Therefore, this technique could inhibit bone destruction and inflammation from TB effectively , better than our previous prepared DDSs. The encapsulating technology, instant hydrogelation of drug-loaded implants, shows potential for regulating the type and ratio of drugs, elastic and viscous modulus of the hydrogel according to the state of illness intraoperatively for optimal drug release.
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http://dx.doi.org/10.1039/d1tb00997dDOI Listing
September 2021

A double helical 4H assembly pattern with secondary hierarchical complexity in an Ag nanocluster crystal.

Nanoscale Horiz 2021 Sep 6. Epub 2021 Sep 6.

Institutes of Physical Science and Information Technology, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei, Anhui, 230601, China.

The hierarchical assemblies of well-defined structural nanoclusters can help to better understand those of biologically important molecules such as DNA and proteins. Herein, we disclose the synthesis and characterization of a new silver nanocluster, that is Ag(SR)(PPh) (Ag70-TPP). Directed by the ligands, Ag70-TPP nanoclusters undergo self-hierarchical assembly into a highly space-efficient complex secondary structure of a double helical 4H (DH4H) close packing pattern. The chirality of Ag70-TPP, and the van der Waals forces interactions between the ligands are believed to drive its DH4H arrangement, and the observed interlocking of the phosphine ligands of adjacent Ag70-TPP nanoclusters also contributed. Overall, this work has yielded important and unprecedented insights into the internal structure and crystallographic arrangement of nanoclusters.
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http://dx.doi.org/10.1039/d1nh00332aDOI Listing
September 2021

A Potential Method for Rapid Screening of Amphioxus Founder Harboring Germline Mutation and Transgene.

Front Cell Dev Biol 2021 12;9:702290. Epub 2021 Aug 12.

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, China.

Amphioxus is a promising model organism for understanding the origin and evolution of vertebrates due to its basal phylogenetic position among chordates. We here compared the mutation efficacy and mutation type of tail tips and gametes of amphioxus founders injected with Cas9 protein and six different sgRNAs targeting five distinct genes, and revealed a strong correlation for mutation efficacy and a mild correlation for mutation type among the two tissues. In addition, we also observed a positive relationship between gene insertions observed in tail tips and gametes of amphioxus founders injected with Tol2 transposase and two different transgenic constructs. Finally, we showed that amphioxus larvae which had their tail tips cut at the 3-4 gill-slit stage were able to recover within 6 days and developed a normal number of gonads at the adult stage, and that F0 larvae carry similar mutation efficacy and type in the posterior end to that in the tail tips after their metamorphosis. Together, these findings suggest a great potential for obtaining valid amphioxus founders with desired mutations and transgenes at as early as the early larval stage, which will certainly speed up the generation of amphioxus mutants and transgenes and make it more cost- and labor-effective.
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http://dx.doi.org/10.3389/fcell.2021.702290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387717PMC
August 2021

Research on the Fastest Detection Method for Weak Trends under Noise Interference.

Entropy (Basel) 2021 Aug 22;23(8). Epub 2021 Aug 22.

School of Electrical Engineering, Zhengzhou University, Zhengzhou 450001, China.

Trend anomaly detection is the practice of comparing and analyzing current and historical data trends to detect real-time abnormalities in online industrial data-streams. It has the advantages of tracking a concept drift automatically and predicting trend changes in the shortest time, making it important both for algorithmic research and industry. However, industrial data streams contain considerable noise that interferes with detecting weak anomalies. In this paper, the fastest detection algorithm "sliding nesting" is adopted. It is based on calculating the data weight in each window by applying variable weights, while maintaining the method of trend-effective integration accumulation. The new algorithm changes the traditional calculation method of the trend anomaly detection score, which calculates the score in a short window. This algorithm, SNWFD-DS, can detect weak trend abnormalities in the presence of noise interference. Compared with other methods, it has significant advantages. An on-site oil drilling data test shows that this method can significantly reduce delays compared with other methods and can improve the detection accuracy of weak trend anomalies under noise interference.
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http://dx.doi.org/10.3390/e23081093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392765PMC
August 2021

Pitx controls amphioxus asymmetric morphogenesis by promoting left-side development and repressing right-side formation.

BMC Biol 2021 Aug 20;19(1):166. Epub 2021 Aug 20.

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan District, Xiamen, 361102, Fujian, China.

Background: Left-right (LR) asymmetry is an essential feature of bilateral animals. Studies in vertebrates show that LR asymmetry formation comprises three major steps: symmetry breaking, asymmetric gene expression, and LR morphogenesis. Although much progress has been made in the first two events, mechanisms underlying asymmetric morphogenesis remain largely unknown due to the complex developmental processes deployed by vertebrate organs.

Results: We here addressed this question by studying Pitx gene function in the basal chordate amphioxus whose asymmetric organogenesis, unlike that in vertebrates, occurs essentially in situ and does not rely on cell migration. Pitx null mutation in amphioxus causes loss of all left-sided organs and incomplete ectopic formation of all right-sided organs on the left side, whereas Pitx partial loss-of-function leads to milder phenotypes with only some LR organs lost or ectopically formed. At the N1 to N3 stages, Pitx expression is gradually expanded from the dorsal anterior domain to surrounding regions. This leads to activation of genes like Lhx3 and/or Prop1 and Pit, which are essential for left-side organs, and downregulation of genes like Hex and/or Nkx2.1 and FoxE4, which are required for right-side organs to form ectopically on the left side. In Pitx mutants, the left-side expressed genes are not activated, while the right-side genes fail to decrease expression on the left side. In contrast, in embryos overexpressing Pitx genes, the left-side genes are induced ectopically on the right side, and the right-side genes are inhibited. Several Pitx binding sites are identified in the upstream sequences of the left-side and right-side genes which are essential for activation of the former and repression of the latter by Pitx.

Conclusions: Our results demonstrate that (1) Pitx is a major (although not the only) determinant of asymmetric morphogenesis in amphioxus, (2) the development of different LR organs have distinct requirements for Pitx activity, and (3) Pitx controls amphioxus LR morphogenesis probably through inducing left-side organs and inhibiting right-side organs directly. These findings show much more dependence of LR organogenesis on Pitx in amphioxus than in vertebrates. They also provide insight into the molecular developmental mechanism of some vertebrate LR organs like the lungs and atria, since they show a right-isomerism phenotype in Pitx2 knockout mice like right-sided organs in Pitx mutant amphioxus. Our results also explain why some organs like the adenohypophysis are asymmetrically located in amphioxus but symmetrically positioned in vertebrates.
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http://dx.doi.org/10.1186/s12915-021-01095-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377849PMC
August 2021

miR-339-5p Inhibits Autophagy to Reduce the Resistance of Laryngeal Carcinoma on Cisplatin via Targeting TAK1.

Biomed Res Int 2021 3;2021:9938515. Epub 2021 Aug 3.

Department of Otorhinolaryngology-Head and Neck Surgery, The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225001, China.

Laryngeal carcinoma is a malignant disease with high morbidity and mortality. Several studies have indicated that miRNA dysfunction involves in the development of laryngeal carcinoma. In this study, the connection of miR-339-5p and laryngeal carcinoma was investigated, and qRT-PCR, CCK-8, and flow cytometry assay were used to observe the function of miR-339-5p on laryngeal carcinoma. Besides, the target database, dual-luciferase reporter assay, and western blot were used to explore the regulation mechanism of miR-339-5p on the progression of laryngeal carcinoma. The results showed that miR-339-5p was significantly downregulated in cisplatin-resistant cells of laryngeal carcinoma, and miR-339-5p upregulation could weaken the resistance of laryngeal carcinoma cells on cisplatin. Moreover, miR-339-5p could directly react with 3'-UTR of TAK1, and TAK1 could reverse the effects of miR-339-5p on the progression of autophagy. In conclusion, this study suggests that miR-339-5p can inhibit the autophagy to decrease the cisplatin resistance of laryngeal carcinoma via targeting TAK1.
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http://dx.doi.org/10.1155/2021/9938515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357498PMC
August 2021

Large chromosomal deletions and impaired homologous recombination repairing in HEK293T cells exposed to polychlorinated biphenyl 153.

PeerJ 2021 28;9:e11816. Epub 2021 Jul 28.

Tianjin Medical University, Tianjin, China.

Background: Polychlorinated biphenyls (PCBs) are persistent pollutants with carcinogenesis and mutagenesis effects which have been closely associated with PCBs-induced DNA damage. However, the detailed DNA damage events and corresponding pathway alterations under PCBs poisoning is still not well understood.

Methods: Whole-genome sequencing (WGS) and RNA sequencing (RNA-seq) were used to explore genome wide variations and related pathway changes in HEK293T cells that challenged by 15 µM PCB153 for 96 h . Double strand breaks (DSBs) were measured by 53BP1 foci detection, altered pathways were confirmed by quantitative real-time PCR (qPCR).

Results: The results indicated that abundant copy number variations (CNVs), including four duplications and 30 deletions, occurred in PCB153-exposed HEK293T cells. Multiple large fragment deletions (>1 Mb) involving up to 245 Mb regions on many chromosomes. Missense mutations were found in six tumor susceptibility genes, two of which are key members participating in homologous recombination (HR) repair response, and . RNA-seq data showed that PCB153 poisoning apparently suppressedHR repairing genes. Besides, 15 µM PCB153 exposure significantly increased 53BP1 foci formation and effectively reduced , and expression, indicating an elevated DSBs and impaired HR repairing.

Conclusion: This study firstly reported multiple large chromosomal deletions and impaired HR repairing in PCB153-exposed HEK293T cells, which provided a new insight into the understanding of early response and the mechanism underlying PCB153 genotoxicity. The chromosomal instabilities might be related to the impaired HR repairing that induced by PCB153; however, further investigations, especially on actual toxic effects of human body, are needed to confirm such speculation.
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http://dx.doi.org/10.7717/peerj.11816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325425PMC
July 2021

Cross-Linking Cellular Prion Protein Induces Neuronal Type 2-Like Hypersensitivity.

Front Immunol 2021 30;12:639008. Epub 2021 Jul 30.

School of Medicine, Western Sydney University, Campbelltown, NSW, Australia.

Background: Previous reports identified proteins associated with 'apoptosis' following cross-linking PrP with motif-specific anti-PrP antibodies and . The molecular mechanisms underlying this IgG-mediated neurotoxicity and the role of the activated proteins in the apoptotic pathways leading to neuronal death has not been properly defined. Previous reports implicated a number of proteins, including apolipoprotein E, cytoplasmic phospholipase A2, prostaglandin and calpain with anti-PrP antibody-mediated 'apoptosis', however, these proteins are also known to play an important role in allergy. In this study, we investigated whether cross-linking PrP with anti-PrP antibodies stimulates a neuronal allergenic response.

Methods: Initially, we predicted the allergenicity of the epitope sequences associated with 'neurotoxic' anti-PrP antibodies using allergenicity prediction servers. We then investigated whether anti-PrP antibody treatment of mouse primary neurons (MPN), neuroblastoma cells (N2a) and microglia (N11) cell lines lead to a neuronal allergenic response.

Results: studies showed that both tail- and globular-epitopes were allergenic. Specifically, binding regions that contain epitopes for previously reported 'neurotoxic' antibodies such as ICSM18 (146-159), ICSM35 (91-110), POM 1 (138-147) and POM 3 (95-100) lead to activation of allergenic related proteins. Following direct application of anti-PrP antibodies on N2a cells, we identified 4 neuronal allergenic-related proteins when compared with untreated cells. Furthermore, we identified 8 neuronal allergenic-related proteins following treatment of N11 cells with anti-PrP antibodies prior to co-culture with N2a cells when compared with untreated cells. Antibody treatment of MPN or MPN co-cultured with antibody-treated N11 led to identifying 10 and 7 allergenic-related proteins when compared with untreated cells. However, comparison with 3F4 antibody treatment revealed 5 and 4 allergenic-related proteins respectively. Of importance, we showed that the allergenic effects triggered by the anti-PrP antibodies were more potent when antibody-treated microglia were co-cultured with the neuroblastoma cell line. Finally, co-culture of N2a or MPN with N11-treated with anti-PrP antibodies resulted in significant accumulation of NO and IL6 but not TNF-α in the cell culture media supernatant.

Conclusions: This study showed for the first time that anti-PrP antibody binding to PrP triggers a neuronal hypersensitivity response and highlights the important role of microglia in triggering an IgG-mediated neuronal hypersensitivity response. Moreover, this study provides an important impetus for including allergenic assessment of therapeutic antibodies for neurodegenerative disorders to derive safe and targeted biotherapeutics.
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http://dx.doi.org/10.3389/fimmu.2021.639008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361482PMC
July 2021

First known case of successful pressure ulcer treatment in a lung transplant patient with post-COVID-19 pneumonia.

J Wound Care 2021 Aug;30(8):594-597

Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Given the current COVID-19 crisis, multiple clinical manifestations and related complications of COVID-19 disease, especially in lung transplant patients following post-COVID-19 pneumonia, are a major challenge. Herein, we report the therapeutic course of the first reported case of sacrococcyx pressure ulcers (PU) in a 65-year-old male COVID-19 patient who underwent lung transplantation and developed a PU following surgery. We used a combination of regulated negative pressure-assisted wound therapy system (RNPT, six treatment courses, five days per treatment course), a skin tension-relief system (an intraoperative aid in minimising wounds caused by sacrococcygeal PUs) and a gluteus maximus myocutaneous flap to repair sacrococcygeal wounds. This successfully treated case provides a reference point for the treatment of similar cases.
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http://dx.doi.org/10.12968/jowc.2021.30.8.594DOI Listing
August 2021

Qualitative Recognition of Primary Taste Sensation Based on Surface Electromyography.

Sensors (Basel) 2021 Jul 23;21(15). Epub 2021 Jul 23.

State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, China.

Based on surface electromyography (sEMG), a novel recognition method to distinguish six types of human primary taste sensations was developed, and the recognition accuracy was 74.46%. The sEMG signals were acquired under the stimuli of no taste substance, distilled vinegar, white granulated sugar, instant coffee powder, refined salt, and Ajinomoto. Then, signals were preprocessed with the following steps: sample augments, removal of trend items, high-pass filter, and adaptive power frequency notch. Signals were classified with random forest and the classifier gave a five-fold cross-validation accuracy of 74.46%, which manifested the feasibility of the recognition task. To further improve the model performance, we explored the impact of feature dimension, electrode distribution, and subject diversity. Accordingly, we provided an optimized feature combination that reduced the number of feature types from 21 to 4, a preferable selection of electrode positions that reduced the number of channels from 6 to 4, and an analysis of the relation between subject diversity and model performance. This study provides guidance for further research on taste sensation recognition with sEMG.
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http://dx.doi.org/10.3390/s21154994DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348720PMC
July 2021

Uniform Deposition and Effective Confinement of Lithium in Three-Dimensional Interconnected Microchannels for Stable Lithium Metal Anodes.

ACS Appl Mater Interfaces 2021 Aug 9;13(33):39311-39321. Epub 2021 Aug 9.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.

Lithium dendrite formation has hindered the practical implementation of lithium metal batteries with higher energy densities compared with those of conventional lithium-ion batteries. Herein, a nanoconfinement strategy to access dendrite-free lithium metal anodes comprising three-dimensional (3D) hollow porous multi-nanochannel carbon fiber embedded with TiO nanocrystals (HTCNF) is reported. The transport of the lithium ions is facilitated by the 3D architecture. Functioning as nanoseeds, the TiO nanocrystals guide the lithium ions toward forming uniform deposits, which are further confined inside the hollow carbon fibers and the 3D HTCNF layer. Site-selective deposition coupled with the nanoconfinement of lithium metal modifies the Li plating/stripping behavior and effectively suppresses the dendrite growth. The HTCNF-Li cell delivers a stable cycling performance of 1300 h with a voltage hysteresis as low as 6 mV. The assembled HTCNF-Li//LiFePO full cell displays a compelling rate performance and enhanced cycling stability with high capacity retention (90% after 400 cycles at 0.5 C). Our results demonstrate a new and potentially scalable route to resolve the lithium dendrite growth issue for enhanced electrochemical performances, which can be further extended to other metal battery systems.
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http://dx.doi.org/10.1021/acsami.1c09319DOI Listing
August 2021

[The Clinical Characteristics and Prognosis of Patients with Acute Myeloid Leukemia Combine Paroxysmal Nocturnal Hemoglobinuria].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Aug;29(4):1080-1084

Department of Hematology, Xi'an Central Hospital Affiliated to Xi'an Jiaotong University,Xi'an 710003, Shaanxi Province,

Objective: To investigate the clinical characteristics and prognosis of patients with acute myeloid leukemia(AML) combined with paroxysmal nocturnal hemoglobinuria(PNH).

Methods: The clinical data of 13 AML combined with PNH patients treated in our hospital from January 2017 to May 2019 were collected and retrospective analyzed. The complete remission(CR) rate for induction chemotherapy was analyzed. The level of PNH cell before and after chemotherapy were tested by Paired t test. Kaplan-Meier method and multi-factorial Cox regression model were used to analyze the influencing factors of prognosis.

Results: Among the 13 patients, 11 (84.6%) cases were CR after first induction chemotherapy. The median overall survival(OS) time was 17 months(0-30 months), the median progression-free survival(PFS) time was 16 months(2-26 months). There were no significant difference in the number of PNH cell before and after chemotherapy (P>0.05). Multivariate Cox regression analysis showed that age,sex,the level of hemoglobin, platelet were not related to the OS of the patients(P>0.05), the level of WBC, LDH and risk stratification at first diagnosed were related to the OS of the patients(P<0.05). Kaplan-Meier survival analysis showed that the OS rate of AML combined with PNH patients with leukocyte lower than 10×10/L at first diagnosed was better than that of the patients with leukocyte higher than 10×10/L (P=0.0261). The OS rate of patients with low or standard risk was better than the patients with high risk group(P=0.0010).

Conclusion: The patients of AML combined with PNH have higher CR rate after the first induction chemotherapy. The level of WBC and LDH at first diagnosed are the factors that affecting the OS of the patients. The OS of patients with WBC lower than 10×10/L, at first diagnosed low and medium risk are better than the other patients.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.04.010DOI Listing
August 2021

Systematic review and meta-analysis: transplanted hematopoietic stem cells and killer cells on leukemia.

Ann Palliat Med 2021 Jul;10(7):7872-7883

Institute of Hematology, Xi'an Jiaotong University Affiliated to Xi'an Central Hospital, Xi'an, China.

Background: meta-analysis was performed to study the therapeutic effect of hematopoietic stem cell transplantation combined with killer cells (important immune cells of the body) on leukemia, hoping to enhance the postoperative therapeutic efficiency.

Methods: literatures were searched with "Hematopoietic stem cell transplantation", "killer cell", "leukemia", "Cytokine induction", etc. as search terms using Boolean logic search. Review Manager was utilized for meta-analysis after literature screening.

Results: eleven literatures were included, most of which were of low-risk bias (medium-high quality). Through meta-analysis, statistical heterogeneity was found in non-recurring mortality (NRM) between control group and experimental group (Chi2 =15.69, I2=62%, P=0.02). The leukemia-free survival rate between two groups was not heterogeneous (Chi2 =13.16, I2=32%, P=0.16), without considerable difference between groups (Z=1.52, P=0.13). The incidence of graft-versus-host disease (GvHD) between the two groups was statistically heterogeneous (Chi2 =21.38, I2=67%, P=0.003). The incidence of graft-versus-host disease in experimental group was greatly inferior to controls (Z=3.87, P=0.0001).

Discussion: hematopoietic stem cell transplantation combined with killer cells can effectively reduce the incidence of GvHD after stem cell transplantation in patients. The prognosis of transplantation was good, and it had no obvious effect on the overall survival rate and recurrence rate.
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http://dx.doi.org/10.21037/apm-21-1359DOI Listing
July 2021

Comparison between flattening filter-free (FFF) and flattened photon beam VMAT plans for the whole brain radiotherapy (WBRT) with hippocampus sparing.

Asia Pac J Clin Oncol 2021 Jul 31. Epub 2021 Jul 31.

Department of Radiation oncology, The First Hospital of China Medical University, Shenyang, China.

Purpose: To evaluate and investigate the feasibility of flattening filter-free (FFF) beam for the whole-brain radiotherapy (WBRT) with hippocampus sparing.

Methods: Eighteen patients with volumetric-modulated arc therapy (VMAT) plans in FFF and conventional beam modes were included in this study. The prescribed dose was 30 Gy in 10 fractions. The conformity index (CI), heterogeneity index reported by TPS (HI-M), and homogeneity index (HI) for planning target volume (PTV) were evaluated. Subsequently, the following parameters for PTV were calculated and compared: D , D ; the mean dose, maximum dose, and minimal dose for OARs. Plan modulation index, total MUs, and the gamma index were used to evaluate the plan quality.

Results: HI-M results were similar for the two techniques (1.239 vs. 1.247, respectively, p = 0.048); FFF beam plans yielded lower D2% compared to FF beam plans (3,416.3 cGy vs. 3,437.2 cGy, p = 0.22), mean dose (3,177.5 cGy vs. 3,195.2 cGy, p = 0.009), and CI (0.884 vs. 0.876, p = 0.001) for PTV. Significant differences were observed between the two beam modes (FF model vs. FFF model) for the maximum dose (1,612.9 cGy vs. 1,470.2 cGy, respectively, p < 0.001), minimum dose (987.6 cGy vs. 898.8 cGy, respectively, p < 0.001), and the mean dose (1144.4 cGy vs. 1047.3 cGy, respectively, p < 0.001) to the hippocampus, and the maximum dose to the eyes (2,792.6 cGy vs. 2,751.3 cGy, respectively, p < 0.001). The average total MUs for FFF-VMAT plans was significantly greater than FF-VMAT plans. However, differences for the plan modulation index and the gamma index were negligible.

Conclusion: In comparison with FF beam, the FFF beam mode offers a clear benefit with respect to WBRT with hippocampal sparing.
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http://dx.doi.org/10.1111/ajco.13624DOI Listing
July 2021

Anatomical Predictors of Valve Malposition During Self-Expandable Transcatheter Aortic Valve Replacement.

Front Cardiovasc Med 2021 12;8:600356. Epub 2021 Jul 12.

Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

The consequence of valve malposition (VM) during transcatheter aortic valve replacement (TAVR) can be severe, but the determinants of VM with self-expandable TAVR have not been thoroughly evaluated. We aimed to investigate the anatomical predictors of VM during self-expandable TAVR. In this multicenter retrospective study, TAVR was performed using the Venus A-Valve. The baseline, computed tomography, and procedural characteristics along with clinical outcomes were collected. Multivariate logistic regression model and receiver operating characteristic (ROC) curve analyses were performed. A total of 84 consecutive patients (23 with VM) were included. Stepwise regression showed that annulus perimeter/left ventricular outflow tract perimeter (AL ratio) and sinotubular junction (STJ) height were predictors of VM. The ROC curve indicated a moderate strength of AL ratio [area under the curve (AUC) 0.71, cutoff 0.96] and a weak strength of STJ height (AUC 0.69, cutoff 23.8 mm) to predict VM. The combination of both predictors revealed a higher predictive value of VM (AUC 0.77). In multivariate analysis, AL ratio <0.96 [odds ratio (OR) 3.98, = 0.015] and STJ height ≥23.8 mm (OR 4.63, = 0.008) were strong independent predictors of VM. The presence of both predictors was associated with a very high risk of VM (OR 10.67, = 0.002). The rate of moderate-to-severe paravalvular regurgitation was higher in patients with VM at 30 days (26.1 vs. 4.9%, = 0.011). A conical left ventricular outflow tract and tall aortic sinuses were strong anatomical predictors of VM during self-expandable TAVR.
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http://dx.doi.org/10.3389/fcvm.2021.600356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311434PMC
July 2021

Plasma extracellular vesicle delivery of miR-210-3p by targeting ATG7 to promote sepsis-induced acute lung injury by regulating autophagy and activating inflammation.

Exp Mol Med 2021 Jul 28;53(7):1180-1191. Epub 2021 Jul 28.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 430060, Wuhan, P.R. China.

Extracellular vesicles (EVs) can be used for intercellular communication by facilitating the transfer of miRNAs from one cell to a recipient cell. MicroRNA (miR)-210-3p is released into the blood during sepsis, inducing cytokine production and promoting leukocyte migration. Thus, the current study aimed to elucidate the role of plasma EVs in delivering miR-210-3p in sepsis-induced acute lung injury (ALI). Plasma EVs were isolated from septic patients, after which the expression of various inflammatory factors was measured using enzyme-linked immunosorbent assay. Cell viability and apoptosis were measured via cell counting kit-8 and flow cytometry. Transendothelial resistance and fluorescein isothiocyanate fluorescence were used to measure endothelial cell permeability. Matrigel was used to examine the tubulogenesis of endothelial cells. The targeting relationship between miR-210-3p and ATG7 was assessed by dual-luciferase reporter assays. The expression of ATG7 and autophagy-related genes was determined to examine autophagic activation. A sepsis mouse model was established by cecal ligation and puncture (CLP)-induced surgery. The level of miR-210-3p was highly enriched in septic EVs. MiR-210-3p enhanced THP-1 macrophage inflammation, BEAS-2B cell apoptosis, and HLMVEC permeability while inhibiting angiogenesis and cellular activity. MiR-210-3p overexpression reduced ATG7 and LC3II/LC3I expression and increased P62 expression. Improvements in vascular density and autophagosome formation, increased ATG7 expression, and changes in the ratio of LC3II/LC3I were detected, as well as reduced P62 expression, in adenovirus-anti-miR-210-3p treated mice after CLP injury. Taken together, the key findings of the current study demonstrate that plasma EVs carrying miR-210-3p target ATG7 to regulate autophagy and inflammatory activation in a sepsis-induced ALI model.
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http://dx.doi.org/10.1038/s12276-021-00651-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333093PMC
July 2021

PLOD2 Is a Potent Prognostic Marker and Associates with Immune Infiltration in Cervical Cancer.

Biomed Res Int 2021 28;2021:5512340. Epub 2021 Jun 28.

Department of Obstetrics and Gynecology, NanFang Hospital of Southern Medical University, Guangzhou, 510515 Guangdong Province, China.

Background: PLOD2 is overexpressed in diverse tumors and plays a vital role in tumorigenesis. However, the prognostic value of PLOD2 in cervical cancer (CESC) remains unclear.

Methods: PLOD2 expression and CESC patients' survival data were collected from the Oncomine, GEPIA, UALCAN, and Kaplan-Meier Plotter databases; immunohistochemistry (IHC) was used to validate the expression of PLOD2 in CESC; Gene Set Enrichment Analysis was performed using the STRING and DAVID databases; and the correlations between PLOD2 and cancer immune infiltrates were investigated using the TIMER and TISIDB databases.

Results: We found that the expression level of PLOD2 was increased in various cancers, and meta-analysis in the Oncomine database revealed that PLOD2 was significantly upregulated in CESC compared to that in normal tissues ( < 0.001). In addition, the high expression of PLOD2 was closely related to poor overall survival (OS) and disease-free survival (DFS) in patients with CESC (OS HR = 1.73, = 0.029; DFS HR = 2.60, = 0.018). Functional annotations indicated that differentially expressed PLOD2 were primarily related to protein digestion and absorption pathways and to the collagen fibril organization process. Immune infiltration analysis showed that PLOD2 was highly correlated with B cells, CD4+ T cells, T helper type 2 (Th2) cells, and eosinophils in CESC.

Conclusion: PLOD2 is positively associated with poor prognosis and might be considered a novel diagnostic and prognostic marker for CESC patients.
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http://dx.doi.org/10.1155/2021/5512340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260295PMC
June 2021

A multi-emission analysis of organic and inorganic pollutants during the combustion of sludge with high and low calorific value coals.

Environ Sci Pollut Res Int 2021 Jul 7. Epub 2021 Jul 7.

College of Environment, Hohai University, No. 1 Xikang Road, Nanjing, 210098, China.

To achieve allowable gas emission limits and eliminate the high cost and challenges associated with pre-treatment of sludge before incineration, a new technique was proposed to co-incinerate wet printing and dyeing sludge with different calorific values of coal while conducting a full evaluation of organic and inorganic emission of pollutants. Different proportions (0%, 10%, 20%, 30%, 40%) of the wet sludge-coal slurry (peat) were incinerated using a commercialized fluid bed (circulating) incinerator. The results showed the that flue gas emitted contained sulfur oxides (SO), nitrogen oxides (NO), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs), and heavy metals in bottom ash. The ΣPCBs content produced from the 7 congeners was 6.4014 μg/m with more than 65% below tetra-chlorinated biphenyls which are the most harmful and persistent. An increment in the sludge (peat) ratio caused the content of SO to decrease drastically, while the content of NO exhibited two maxima. The heavy metal concentrations of Cd, Zn, Mn, Pb, Fe, Ni, Cr, and Cu decreased with increasing sludge ratio. The leaching toxicity of heavy metals in the bottom ash proved to be lower than the limit values for hazardous waste. The PCDD/F 1-TEQ emissions of the flue gas increased from 0.06844 to 0.10779 ng/m as the proportion of sludge increased in peat. The I-TEQ values of the PCDD/Fs showed a slight increment with increasing sludge ratio. The sum of the PCDD/Fs (ideally at 20-30%) does not exceed the 0.1ng/Nm of the EU and the 0.5 ng TEQ/Nm of the new Chinese emissions standard. Statement of novelty Different calorific values of coal of different proportions were incinerated with wet printing and dyeing sludge in order to achieve the new allowable gas emission limits, tap heat energy and eliminate high cost.
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http://dx.doi.org/10.1007/s11356-021-15301-7DOI Listing
July 2021

Improving wheat grain yield via promotion of water and nitrogen utilization in arid areas.

Sci Rep 2021 Jul 5;11(1):13821. Epub 2021 Jul 5.

Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, 730070, China.

Crop yield is limited by water and nitrogen (N) availability. However, in Hexi Corridor of northwestern China, water scarcity and excessive fertilizer N in wheat (Triticum aestivum L.) production causes serious conflicts between water and N supply and crop demand. A field experiment was conducted from 2016 to 2018 to evaluate whether reducing of irrigation and fertilizer N will reduce grain yield of wheat. There were two irrigation quotas (192 and 240 mm) and three fertilizer N rates (135, 180, and 225 kg N ha). The results showed that reducing irrigation to 192 mm and N rate to 180 kg N ha reduced water uptake, water uptake efficiency, and N uptake of spring wheat as compared to local practice (i.e., 240 mm irrigation and 225 kg N ha fertilizer). Whereas, it improved water and N utilization efficiency, and water and N productivity. Consequently, the irrigation and N rate reduced treatment achieved the same quantity of grain yield as local practice. The path analysis showed that interaction effect between irrigation and N fertilization may attributable to the improvement of grain yield with lower irrigation and N rate. The enhanced water and N utilization allows us to conclude that irrigation quota at 192 mm coupled with fertilizer N rate at 180 kg N ha can be used as an efficient practice for wheat production in arid irrigation areas.
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http://dx.doi.org/10.1038/s41598-021-92894-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257629PMC
July 2021

Screening of Candidate Pathogenic Genes for Spontaneous Abortion using Whole Exome Sequencing.

Comb Chem High Throughput Screen 2021 Jun 27. Epub 2021 Jun 27.

Shanghai Biological Information Research Center, Zhangjiang Hi-tech Park, Shanghai, 201203, China.

Background: Spontaneous abortion is a common disease in obstetrics and reproduction.

Objective: This study aimed to screen candidate pathogenic genes for spontaneous abortion using whole-exome sequencing.

Methods: Genomic DNA was extracted from abortion tissues of spontaneous abortion patients and sequenced using the Illumina HiSeq2500 high-throughput sequencing platform. Whole exome sequencing was performed to select harmful mutations, including SNP and insertion and deletion sites, associated with spontaneous abortion. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses and gene fusion analyses were performed. MUC3A and PDE4DIP were two novel mutation genes that were screened and verified by PCR in abortion tissues of patients.

Results: A total of 83,633 SNPs and 13,635 Indel mutations were detected, of which 29172 SNPs and 3093 Indels were screened as harmful mutations. The 7 GO-BP, 4 GO-CC, 9 GO-MF progress, and 3 KEGG pathways were enriched in GO and KEGG pathway analyses. A total of 746 gene fusion mutations were obtained, involving 492 genes. MUC3A and PDE4DIP were used for PCR verification because of their high number of mutation sites in all samples.

Conclusion: There are extensive SNPs and Indel mutations in the genome of spontaneous abortion tissues, and the effect of these gene mutations on spontaneous abortion needs further experimental verification.
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http://dx.doi.org/10.2174/1386207324666210628115715DOI Listing
June 2021

Shock Index-C: An Updated and Simple Risk-Stratifying Tool in ST-Segment Elevation Myocardial Infarction.

Front Cardiovasc Med 2021 15;8:657817. Epub 2021 Jun 15.

Guangdong Provincial Key Laboratory of Coronary Heart Disease Prevention, Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Shock index (heart rate/systolic blood pressure, SI) is a simple scale with prognostic value in patients with ST-segment elevation myocardial infarction (STEMI) undergoing percutaneous coronary intervention (PCI). The present study introduces an updated version of SI that includes renal function. A total of 1,851 consecutive patients with STEMI undergoing PCI were retrospectively included at Cardiac Care Unit in Guangdong Provincial People's Hospital and divided into two groups according to their admission time: derivation database (from January 2010 to December 2013, = 1,145) and validation database (from January 2014 to April 2016, = 706). Shock Index-C (SIC) was calculated as (SI × 100)-estimated CCr. Calibration was evaluated using the Hosmer-Lemeshow statistic. The predictive power of SIC was evaluated using receiver operating characteristic (ROC) curve analysis. The predictive value and calibration of SIC for in-hospital death was excellent in derivation [area under the curve (AUC) = 0.877, < 0.001; Hosmer-Lemeshow chi-square = 3.95, = 0.861] and validation cohort (AUC = 0.868, < 0.001; Hosmer-Lemeshow chi-square = 5.01, = 0.756). SIC exhibited better predictive power for in-hospital events than SI (AUC: 0.874 vs. 0.759 for death; 0.837 vs. 0.651 for major adverse clinical events [MACEs]; 0.707 vs. 0.577 for contrast-induced acute kidney injury [CI-AKI]; and 0.732 vs. 0.590 for bleeding, all < 0.001). Cumulative 1-year mortality was significantly higher in the upper SIC tertile (log-rank = 131.89, < 0.001). SIC was an effective predictor of poor prognosis and may have potential as a novel and simple risk stratification tool for patients with STEMI undergoing PCI.
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http://dx.doi.org/10.3389/fcvm.2021.657817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241092PMC
June 2021

Eight soybean reference genome resources from varying latitudes and agronomic traits.

Sci Data 2021 07 1;8(1):164. Epub 2021 Jul 1.

Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China.

Comparative analysis of multiple reference genomes representing diverse genetic backgrounds is critical for understanding the role of key alleles important in domestication and genetic breeding of important crops such as soybean. To enrich the genetic resources for soybean, we describe the generation, technical assessment, and preliminary genomic variation analysis of eight de novo reference-grade soybean genome assemblies from wild and cultivated accessions. These resources represent soybeans cultured at different latitudes and exhibiting different agronomical traits. Of these eight soybeans, five are from new accessions that have not been sequenced before. We demonstrate the usage of these genomes to identify small and large genomic variations affecting known genes as well as screening for genic PAV regions for identifying candidates for further functional studies.
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http://dx.doi.org/10.1038/s41597-021-00947-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249447PMC
July 2021

A Robust and Rapid Candidate Gene Mapping Pipeline Based on M2 Populations.

Front Plant Sci 2021 2;12:681816. Epub 2021 Jun 2.

Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China.

The whole-genome sequencing-based bulked segregant analysis (WGS-BSA) has facilitated the mapping candidate causal variations for cloning target plant genes. Here, we report an improved WGS-BSA method termed as M2-seq to expedite the mapping candidate mutant loci by studying just M generation. It is an efficient mutant gene mapping tool, rapid, and comparable to the previously reported approaches, such as Mutmap and Mutmap+ that require studying M or advanced selfed generations. In M2-seq, background variations among the M populations can be removed efficiently without knowledge of the variations of the wild-type progenitor plant. Furthermore, the use of absolute delta single-nucleotide polymorphism (SNP) index values can effectively remove the background variation caused by repulsion phase linkages of adjacent mutant alleles; and thereby facilitating the identification of the causal mutation in target genes. Here, we demonstrated the application of M2-seq in successfully mapping the genomic regions harboring causal mutations for mutant phenotypes among 10 independent M populations of soybean. The mapping candidate mutant genes just in M generation with the aid of the M2-seq method should be particularly useful in expediting gene cloning especially among the plant species with long generation time.
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http://dx.doi.org/10.3389/fpls.2021.681816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207192PMC
June 2021

Lipocalin 10 as a New Prognostic Biomarker in Sepsis-Induced Myocardial Dysfunction and Mortality: A Pilot Study.

Mediators Inflamm 2021 22;2021:6616270. Epub 2021 May 22.

Department of Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China.

Introduction: Sepsis-induced myocardial dysfunction (SIMD) is the most common complications of sepsis and septic shock with extremely high incidence and mortality. Lipocalin 10 (Lcn10) has recently been identified as a potential biomarker for heart failure, yet its relation to sepsis has not been investigated. The purpose of this study was to explore whether circulating Lcn10 could be used as a prognostic tool in patients with SIMD.

Methods: In this single-center observational pilot study, seventy-five sepsis patients were enrolled after sepsis diagnosis or ICU admission (45.3% female, median age 60 years), and 35 patients (46.7%) developed myocardial dysfunction. Serum Lcn10 levels of septic patients were measured using the enzyme-linked immunosorbent assay (ELISA) at the time of admission. Other biomarkers of cardiac function and Lcn10 concentration were compared between SIMD and non-SIMD groups.

Results: We observed that the median Lcn10 levels were 2.780 ng/mL in patients with SIMD and 2.075 ng/mL in patients without SIMD ( < 0.05). The area under the receiver operating characteristic (ROC) curve for the diagnosis of SIMD was 0.797 ( < 0.05). In addition, elevated serum Lcn10 levels at the time of admission were positively associated with 28-day mortality in septic patients.

Conclusions: Our study indicates that circulating Lcn10 levels may serve as a novel biomarker for the diagnosis and prognosis of myocardial dysfunction induced by sepsis. An additional large multicenter study may be warranted to confirm the findings of this study.
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http://dx.doi.org/10.1155/2021/6616270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166480PMC
May 2021

Overexpression of human BAG3 in mice causes restrictive cardiomyopathy.

Nat Commun 2021 06 11;12(1):3575. Epub 2021 Jun 11.

Institute of Physiology I, Life and Brain Center, Medical Faculty, University of Bonn, Bonn, Germany.

An amino acid exchange (P209L) in the HSPB8 binding site of the human co-chaperone BAG3 gives rise to severe childhood cardiomyopathy. To phenocopy the disease in mice and gain insight into its mechanisms, we generated humanized transgenic mouse models. Expression of human BAG3-eGFP in mice caused Z-disc disintegration and formation of protein aggregates. This was accompanied by massive fibrosis resulting in early-onset restrictive cardiomyopathy with increased mortality as observed in patients. RNA-Seq and proteomics revealed changes in the protein quality control system and increased autophagy in hearts from hBAG3-eGFP mice. The mutation renders hBAG3 less soluble in vivo and induces protein aggregation, but does not abrogate hBAG3 binding properties. In conclusion, we report a mouse model mimicking the human disease. Our data suggest that the disease mechanism is due to accumulation of hBAG3 and mouse Bag3, causing sequestering of components of the protein quality control system and autophagy machinery leading to sarcomere disruption.
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http://dx.doi.org/10.1038/s41467-021-23858-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196106PMC
June 2021

Vertical and seasonal changes in soil carbon pools to vegetation degradation in a wet meadow on the Qinghai-Tibet Plateau.

Sci Rep 2021 Jun 10;11(1):12268. Epub 2021 Jun 10.

College of Forestry, Gansu Agricultural University, Lanzhou, 730070, China.

Wet meadows provide opportunities to decrease carbon dioxide (CO) and methane (CH) released into the atmosphere by increasing the soil organic carbon (SOC) stored in wetland systems. Although wet meadows serve as the most important and stable C sinks, there has been very few investigations on the seasonal distributions of SOC fractions in high-altitude wet meadows. Here, we studied the effects of four vegetation degradation levels, non-degraded (ND), lightly degraded (LD), moderately degraded (MD), and heavily degraded (HD), on the measured vertical and seasonal changes of SOC and its different fractions. Among these vegetation degradation levels, 0-10 and 10-20 cm soil depths in ND plots had significantly higher SOC contents than the other degradation levels had throughout the year. This is attributed to the relatively greater inputs of aboveground plant litter and richer fine-root biomass in ND plots. Particulate organic carbon (POC) and light fraction organic carbon (LFOC) showed similar vertical and seasonal variations in autumn, reaching a minimum. Moreover, microbial biomass (MBC) and easily oxidizable organic carbon (EOC) contents were highest in summer and the smallest in winter, while dissolved organic carbon (DOC) content was highest in spring and lowest in summer, and were mainly concentrated in the 0-20 cm layer. Pearson correlation analysis indicated that soil properties and aboveground biomass were significantly related to different SOC fractions. The results indicate that vegetation degradation reduces the accumulation of total SOC and its different fractions, which may reduce carbon sink capacity and soil quality of alpine wet meadows, and increase atmospheric environmental pressure. In addition, vegetation biomass and soil characteristics play a key role in the formation and transformation of soil carbon. These results strengthen our understanding of soil C dynamics, specifically related to the different C fractions as affected by vegetation degradation levels and soil depth, in wet meadow systems.
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http://dx.doi.org/10.1038/s41598-021-90543-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192520PMC
June 2021

Zn, Co, and Fe Tridoped N-C Core-Shell Nanocages as the High-Efficiency Oxygen Reduction Reaction Electrocatalyst in Zinc-Air Batteries.

ACS Appl Mater Interfaces 2021 Jun 9;13(24):28324-28333. Epub 2021 Jun 9.

National Base for International Science & Technology Cooperation, National Local Joint Engineering Laboratory for Key Materials of New Energy Storage Battery, Hunan Province Key Laboratory of Electrochemical Energy Storage & Conversion, School of Chemistry, Xiangtan University, Xiangtan 411105, China.

Transition metal-nitrogen-carbon (TM-N-C) nanomaterials are promising platinum-based substitutes for the oxygen reduction reaction (ORR). However, large-scale commercial production of high-efficiency, durable TM-N-C catalysts remains a formidable challenge. In this work, a facile ″ZIF-on-ZIF″ strategy is first adopted to design [email protected] core-shell polyhedral nanocages, and then, ferrocene (Fc) is added to form [email protected]@Fc double-layer encapsulating polyhedral nanocages. Finally, Zn, Co, and Fe tridoped N-C nanocages (ZnCoFe-N-C) as the high-efficiency ORR electrocatalyst are prepared through high-temperature annealing. Benefiting from the trimetal, nitrogen and carbon species bond to each other to form highly efficient active sites, and the material exhibits outstanding performance in 0.1 M KOH, onset potential and half-wave potential of up to 0.95 and 0.878 V (vs RHE), respectively, and long-term durability and methanol tolerance. Furthermore, when utilizing as a zinc-air battery (ZAB) air electrode, it exhibits wonderful indicators, reflected in an open circuit voltage of 1.525 V, power density of 350.2 mW cm, and specific capacity of 794.7 mAh g, which outperforms the benchmark Pt/C catalyst. This work provides a facile and effective strategy to obtain a highly efficient and stable TM-N-C electrocatalyst for the ORR in ZABs.
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http://dx.doi.org/10.1021/acsami.1c06750DOI Listing
June 2021

CircPRDM2 Contributes to Doxorubicin Resistance of Osteosarcoma by Elevating EZH2 via Sponging miR-760.

Cancer Manag Res 2021 2;13:4433-4445. Epub 2021 Jun 2.

Department of Spine Surgery, Tianjin Union Medical Center, Tianjin, 300121, People's Republic of China.

Background: Circular RNAs (circRNAs) are implicated in the chemoresistance of human cancers. However, the functions of circRNA PR/SET domain 2 (circPRDM2) in the resistance of osteosarcoma (OS) to doxorubicin (DXR) are unknown.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) assay was conducted to determine the levels of circPRDM2, microRNA-760 (miR-760) and enhancer of zeste homolog 2 (EZH2). RNase R assay was used to analyze the characteristics of circPRDM2. IC50 of DXR was estimated by Cell Counting Kit-8 (CCK-8) assay. Colony formation assay was performed for cell colony formation ability. Wound-healing assay and transwell assay were utilized for cell migration and invasion. Flow cytometry analysis was conducted for cell apoptosis. Western blot assay was employed for protein levels. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay were adopted to analyze the relationships among circPRDM2, miR-760 and EZH2. Murine xenograft model assay was utilized to explore DXR resistance in vivo.

Results: CircPRDM2 level was enhanced in DXR-resistant OS tissues and cells. CircPRDM2 deficiency inhibited IC50 of DXR, colony formation, migration and invasion and facilitated apoptosis in DXR-resistant OS cells in vitro. CircPRDM2 was identified as the sponge for miR-760. MiR-760 inhibition reversed the inhibitory effects of circPRDM2 knockdown on DXR resistance and cell progression in DXR-resistant OS cells. Moreover, EZH2 was identified as the target gene of miR-760 and EZH2 overexpression abolished miR-760-mediated impacts on DXR sensitivity and malignant behaviors in DXR-resistant OS cells. Also, circPRDM2 silencing improved DXR sensitivity in vivo.

Conclusion: Our study demonstrated the role of circPRDM2/miR-760/EZH2 axis in enhancing DXR resistance.
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http://dx.doi.org/10.2147/CMAR.S295147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180268PMC
June 2021
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