Dr. Guan Wang, PhD - East China university of Science and Technology

Dr. Guan Wang

PhD

East China university of Science and Technology

Shanghai | China

Additional Specialties: biotechnology and bioengineering

Dr. Guan Wang, PhD - East China university of Science and Technology

Dr. Guan Wang

PhD

Introduction

Primary Affiliation: East China university of Science and Technology - Shanghai , China

Additional Specialties:

Publications

3Publications

71Reads

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4PubMed Central Citations

Advances and Practices of Bioprocess Scale-up.

Adv Biochem Eng Biotechnol 2016 ;152:137-51

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

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http://dx.doi.org/10.1007/10_2014_293DOI Listing
February 2016
49 Reads
1 Citation
1.657 Impact Factor

integration of microbial kinetics and fluid dynamics towards model-driven scale-up of industrial bioprocesses

Engineering in life sciences

Scale-up of bioprocesses is hampered by open questions, mostly related to poor mixing and mass transfer limitations. Concentration gradients of substrate, carbon dioxide, and oxygen in time and space, especially in large-scale high-cell density fed-batch processes, are likely induced as the mixing time of the fermentor is usually longer than the relevant cellular reaction time. Cells in the fermentor are therefore repeatedly exposed to dynamic environments or perturbations. As a consequence, the heterogeneity in industrial practices often decreases either yield, titer, or productivity, or combinations thereof and increases by-product formation as compared to well-mixed small-scale bioreactors, which is summarized as scale-up effects. Identification of response mechanisms of the microorganism to various external perturbations is of great importance for pinpointing metabolic bottlenecks and targets for metabolic engineering. In this review, pulse response experimentation is proposed as an ideal way of obtaining kinetic information in combination with scale-down approaches for in-depth understanding of dynamic response mechanisms. As an emerging tool, computational fluid dynamics is able to draw a holistic picture of the fluid flow and concentration fields in the fermentor and finds its use in the optimization of fermentor design and process strategy. In the future, directed strain improvement and fermentor redesign are expected to largely depend on models, in which both microbial kinetics and fluid dynamics are thoroughly integrated.

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December 2014
46 Reads

Prelude to rational scale-up of penicillin production: a scale-down study.

Appl Microbiol Biotechnol 2014 Mar 12;98(6):2359-69. Epub 2014 Jan 12.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai, 200237, People's Republic of China.

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http://dx.doi.org/10.1007/s00253-013-5497-2DOI Listing
March 2014
22 Reads
3 Citations
3.337 Impact Factor

Top co-authors

Siliang Zhang
Siliang Zhang

State Key Laboratory of Bioreactor Engineering

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Jianye Xia
Jianye Xia

State Key Laboratory of Bioreactor Engineering

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Yingping Zhuang
Yingping Zhuang

East China University of Science and Technology

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Ju Chu
Ju Chu

East China University of Science and Technology

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Wenjun Tang
Wenjun Tang

The Pennsylvania State University

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DSM Biotechnology Center

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Delft University of Technology