Gregory Stephanopoulos

Gregory Stephanopoulos

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Protein engineering strategies for microbial production of isoprenoids.

Metab Eng Commun 2020 Dec 16;11:e00129. Epub 2020 May 16.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States.

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http://dx.doi.org/10.1016/j.mec.2020.e00129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322351PMC
December 2020

Novel Strategies and Platforms for Industrial Isoprenoid Engineering.

Trends Biotechnol 2020 Jul 29;38(7):811-822. Epub 2020 Apr 29.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.tibtech.2020.03.009DOI Listing
July 2020

Mixed carbon substrates: a necessary nuisance or a missed opportunity?

Curr Opin Biotechnol 2020 Apr 9;62:15-21. Epub 2019 Sep 9.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, USA. Electronic address:

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http://dx.doi.org/10.1016/j.copbio.2019.07.003DOI Listing
April 2020

Using biopolymer bodies for encapsulation of hydrophobic products in bacterium.

Metab Eng 2020 Apr 24. Epub 2020 Apr 24.

Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore; Disruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2020.04.006DOI Listing
April 2020

Engineering Yarrowia lipolytica for the utilization of acid whey.

Metab Eng 2020 01 25;57:43-50. Epub 2019 Sep 25.

Massachusetts Institute of Technology, 77, Massachusetts Avenue, Cambridge, MA, 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2019.09.010DOI Listing
January 2020

Cell free biosynthesis of isoprenoids from isopentenol.

Biotechnol Bioeng 2019 12 3;116(12):3269-3281. Epub 2019 Sep 3.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts.

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http://dx.doi.org/10.1002/bit.27146DOI Listing
December 2019

Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast.

Cell Syst 2019 12 13;9(6):534-547.e5. Epub 2019 Nov 13.

Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA; Andlinger Center for Energy and the Environment, Princeton University, Princeton, NJ, USA; Department of Molecular Biology, Princeton University, Princeton, NJ, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cels.2019.10.006DOI Listing
December 2019

Limitations in converting waste gases to fuels and chemicals.

Curr Opin Biotechnol 2019 10 8;59:39-45. Epub 2019 Mar 8.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.copbio.2019.02.004DOI Listing
October 2019

Engineering Corynebacterium glutamicum for high-titer biosynthesis of hyaluronic acid.

Metab Eng 2019 09 10;55:276-289. Epub 2019 Jul 10.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, United States. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2019.07.003DOI Listing
September 2019

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2.

ACS Synth Biol 2019 04 20;8(4):796-806. Epub 2019 Mar 20.

Merkin Institute of Transformative Technologies in Healthcare , Broad Institute of MIT and Harvard , Cambridge , Massachusetts 02142 , United States.

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http://dx.doi.org/10.1021/acssynbio.8b00481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479731PMC
April 2019

Enhancing hydrogen-dependent growth of and carbon dioxide fixation by Clostridium ljungdahlii through nitrate supplementation.

Biotechnol Bioeng 2019 02 9;116(2):294-306. Epub 2018 Dec 9.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts.

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http://doi.wiley.com/10.1002/bit.26847
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http://dx.doi.org/10.1002/bit.26847DOI Listing
February 2019

Biosynthesis of monoethylene glycol in Saccharomyces cerevisiae utilizing native glycolytic enzymes.

Metab Eng 2019 01 27;51:20-31. Epub 2018 Sep 27.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S10967176183023
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http://dx.doi.org/10.1016/j.ymben.2018.09.012DOI Listing
January 2019

Two-step pathway for isoprenoid synthesis.

Proc Natl Acad Sci U S A 2019 01 24;116(2):506-511. Epub 2018 Dec 24.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;

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http://dx.doi.org/10.1073/pnas.1812935116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329939PMC
January 2019

Holistic Approaches in Lipid Production by Yarrowia lipolytica.

Trends Biotechnol 2018 11 11;36(11):1157-1170. Epub 2018 Jul 11.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.tibtech.2018.06.007DOI Listing
November 2018

Metabolic engineering in the host Yarrowia lipolytica.

Metab Eng 2018 11 26;50:192-208. Epub 2018 Jul 26.

McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712, United States. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S10967176183027
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http://dx.doi.org/10.1016/j.ymben.2018.07.016DOI Listing
November 2018

Metabolic engineering of Escherichia coli for the production of L-malate from xylose.

Metab Eng 2018 07 22;48:25-32. Epub 2018 May 22.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S10967176183008
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http://dx.doi.org/10.1016/j.ymben.2018.05.010DOI Listing
July 2018

Rediverting carbon flux in Clostridium ljungdahlii using CRISPR interference (CRISPRi).

Metab Eng 2018 07 15;48:243-253. Epub 2018 Jun 15.

Department of Chemical Engineering, Massachusetts Institute of Technology, 25 Ames Street, MIT 56-469 C, Cambridge, MA 02139, United States. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2018.06.006DOI Listing
July 2018

Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas.

Biotechnol Bioeng 2018 06 24;115(6):1450-1464. Epub 2018 Mar 24.

Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts.

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http://doi.wiley.com/10.1002/bit.26576
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http://dx.doi.org/10.1002/bit.26576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947824PMC
June 2018

Improving formaldehyde consumption drives methanol assimilation in engineered E. coli.

Nat Commun 2018 06 19;9(1):2387. Epub 2018 Jun 19.

Department of Chemical Engineering, Massachusetts Institute of Technology, 25 Ames Street, MIT 56-469C, Cambridge, MA, 02139, USA.

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http://www.nature.com/articles/s41467-018-04795-4
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http://dx.doi.org/10.1038/s41467-018-04795-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6008399PMC
June 2018

Metabolic engineering of Escherichia coli for the production of isoprenoids.

FEMS Microbiol Lett 2018 05;365(10)

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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https://academic.oup.com/femsle/article/doi/10.1093/femsle/f
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http://dx.doi.org/10.1093/femsle/fny079DOI Listing
May 2018

Simple glycolipids of microbes: Chemistry, biological activity and metabolic engineering.

Synth Syst Biotechnol 2018 Mar 15;3(1):3-19. Epub 2017 Dec 15.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA.

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https://linkinghub.elsevier.com/retrieve/pii/S2405805X173009
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http://dx.doi.org/10.1016/j.synbio.2017.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5884252PMC
March 2018

Development of a formaldehyde biosensor with application to synthetic methylotrophy.

Biotechnol Bioeng 2018 Jan 3;115(1):206-215. Epub 2017 Nov 3.

Department of Chemical Engineering, MIT, Cambridge, MA 02139, USA.

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http://doi.wiley.com/10.1002/bit.26455
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http://dx.doi.org/10.1002/bit.26455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5699943PMC
January 2018

Metabolic engineering of Escherichia coli for the synthesis of the quadripolymer poly(glycolate-co-lactate-co-3-hydroxybutyrate-co-4-hydroxybutyrate) from glucose.

Metab Eng 2017 11 13;44:38-44. Epub 2017 Sep 13.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2017.09.003DOI Listing
November 2017

Enhanced Biosynthesis of Hyaluronic Acid Using Engineered Corynebacterium glutamicum Via Metabolic Pathway Regulation.

Biotechnol J 2017 Oct 28;12(10). Epub 2017 Sep 28.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://doi.wiley.com/10.1002/biot.201700191
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http://dx.doi.org/10.1002/biot.201700191DOI Listing
October 2017

Review of metabolic pathways activated in cancer cells as determined through isotopic labeling and network analysis.

Metab Eng 2017 09 10;43(Pt B):113-124. Epub 2017 Feb 10.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S10967176173005
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http://dx.doi.org/10.1016/j.ymben.2017.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552450PMC
September 2017

In Vitro Metabolic Engineering of Amorpha-4,11-diene Biosynthesis at Enhanced Rate and Specific Yield of Production.

ACS Synth Biol 2017 09 25;6(9):1691-1700. Epub 2017 May 25.

Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance , Singapore 138602.

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http://pubs.acs.org/doi/10.1021/acssynbio.6b00377
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http://dx.doi.org/10.1021/acssynbio.6b00377DOI Listing
September 2017

Exploiting Bioprocessing Fluctuations to Elicit the Mechanistics of De Novo Lipogenesis in Yarrowia lipolytica.

PLoS One 2017 4;12(1):e0168889. Epub 2017 Jan 4.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168889PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5215641PMC
August 2017

Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica.

Biotechnol Bioeng 2017 07 18;114(7):1521-1530. Epub 2017 Apr 18.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.

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http://dx.doi.org/10.1002/bit.26285DOI Listing
July 2017

Designing a New Entry Point into Isoprenoid Metabolism by Exploiting Fructose-6-Phosphate Aldolase Side Reactivity of Escherichia coli.

ACS Synth Biol 2017 07 13;6(7):1416-1426. Epub 2017 Apr 13.

Department of Chemical Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02142, United States.

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http://pubs.acs.org/doi/10.1021/acssynbio.7b00072
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http://dx.doi.org/10.1021/acssynbio.7b00072DOI Listing
July 2017

Application of metabolic controls for the maximization of lipid production in semicontinuous fermentation.

Proc Natl Acad Sci U S A 2017 07 19;114(27):E5308-E5316. Epub 2017 Jun 19.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139;

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http://www.pnas.org/lookup/doi/10.1073/pnas.1703321114
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http://dx.doi.org/10.1073/pnas.1703321114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502627PMC
July 2017

Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production.

J Ind Microbiol Biotechnol 2017 05 8;44(4-5):605-612. Epub 2016 Nov 8.

Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.

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http://link.springer.com/10.1007/s10295-016-1864-1
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http://dx.doi.org/10.1007/s10295-016-1864-1DOI Listing
May 2017

Exploring biochemical pathways for mono-ethylene glycol (MEG) synthesis from synthesis gas.

Metab Eng 2017 05 19;41:173-181. Epub 2017 Apr 19.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2017.04.005DOI Listing
May 2017

Key Role of the Carboxyl Terminus of Hyaluronan Synthase in Processive Synthesis and Size Control of Hyaluronic Acid Polymers.

Biomacromolecules 2017 04 8;18(4):1064-1073. Epub 2017 Mar 8.

Department of Chemical Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

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http://pubs.acs.org/doi/10.1021/acs.biomac.6b01239
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http://dx.doi.org/10.1021/acs.biomac.6b01239DOI Listing
April 2017

Engineering of Taxadiene Synthase for Improved Selectivity and Yield of a Key Taxol Biosynthetic Intermediate.

ACS Synth Biol 2017 02 4;6(2):201-205. Epub 2016 Nov 4.

Whitehead Institute for Biomedical Research , 9 Cambridge Center, Cambridge, Massachusetts 02142, United States.

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http://dx.doi.org/10.1021/acssynbio.6b00206DOI Listing
February 2017

Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism.

Nat Biotechnol 2017 02 16;35(2):173-177. Epub 2017 Jan 16.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nbt.3763DOI Listing
February 2017

Improving Metabolic Pathway Efficiency by Statistical Model-Based Multivariate Regulatory Metabolic Engineering.

ACS Synth Biol 2017 01 15;6(1):148-158. Epub 2016 Aug 15.

Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

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http://pubs.acs.org/doi/10.1021/acssynbio.6b00187
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http://dx.doi.org/10.1021/acssynbio.6b00187DOI Listing
January 2017

C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica.

Metab Eng 2016 11 5;38:86-97. Epub 2016 Jul 5.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2016.06.006DOI Listing
November 2016

Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals.

Proc Natl Acad Sci U S A 2016 09 12;113(39):10848-53. Epub 2016 Sep 12.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139

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http://www.pnas.org/content/113/39/10848.full.pdf
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047176PMC
http://dx.doi.org/10.1073/pnas.1607295113DOI Listing
September 2016

Efflux transporter engineering markedly improves amorphadiene production in Escherichia coli.

Biotechnol Bioeng 2016 08 4;113(8):1755-63. Epub 2016 Feb 4.

Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore, Singapore.

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http://dx.doi.org/10.1002/bit.25943DOI Listing
August 2016

Metabolic engineering of microbial competitive advantage for industrial fermentation processes.

Science 2016 Aug;353(6299):583-6

Novogy, 85 Bolton Street, Cambridge, MA 02140, USA. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

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http://dx.doi.org/10.1126/science.aaf6159DOI Listing
August 2016

Metabolic requirements for cancer cell proliferation.

Cancer Metab 2016 18;4:16. Epub 2016 Aug 18.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 USA.

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http://dx.doi.org/10.1186/s40170-016-0156-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4989334PMC
August 2016

Co-culture engineering for microbial biosynthesis of 3-amino-benzoic acid in Escherichia coli.

Biotechnol J 2016 Jul 17;11(7):981-7. Epub 2016 Jun 17.

Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.

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http://dx.doi.org/10.1002/biot.201600013DOI Listing
July 2016

Biosynthesis of poly(glycolate-co-lactate-co-3-hydroxybutyrate) from glucose by metabolically engineered Escherichia coli.

Metab Eng 2016 May 14;35:1-8. Epub 2016 Jan 14.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2016.01.004DOI Listing
May 2016

Improved Gene Targeting through Cell Cycle Synchronization.

PLoS One 2015 20;10(7):e0133434. Epub 2015 Jul 20.

Novogy Inc., Cambridge, Massachusetts, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133434PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507847PMC
April 2016

Functional overexpression and characterization of lipogenesis-related genes in the oleaginous yeast Yarrowia lipolytica.

Appl Microbiol Biotechnol 2016 Apr 26;100(8):3781-98. Epub 2016 Feb 26.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

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http://dx.doi.org/10.1007/s00253-016-7376-0DOI Listing
April 2016

Integrated bioprocess for conversion of gaseous substrates to liquids.

Proc Natl Acad Sci U S A 2016 Apr 7;113(14):3773-8. Epub 2016 Mar 7.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139

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http://dx.doi.org/10.1073/pnas.1516867113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4833252PMC
April 2016

Accessing Nature's diversity through metabolic engineering and synthetic biology.

F1000Res 2016 24;5. Epub 2016 Mar 24.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

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http://dx.doi.org/10.12688/f1000research.7311.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813638PMC
April 2016

High-titer biosynthesis of hyaluronic acid by recombinant Corynebacterium glutamicum.

Biotechnol J 2016 Mar 27;11(4):574-84. Epub 2016 Jan 27.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

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http://dx.doi.org/10.1002/biot.201500404DOI Listing
March 2016

Efficient utilization of pentoses for bioproduction of the renewable two-carbon compounds ethylene glycol and glycolate.

Metab Eng 2016 Mar 19;34:80-87. Epub 2015 Dec 19.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2015.12.004DOI Listing
March 2016

Letter from AIChE President.

Bioeng Transl Med 2016 Mar 27;1(1). Epub 2016 May 27.

2016 AIChE President, Past Chair, SBE, W.H. Dow Professor of Chemical Engineering and Biotechnology, Massachusetts Institute of Technology.

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http://dx.doi.org/10.1002/btm2.10006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689514PMC
March 2016

Engineering a novel biosynthetic pathway in Escherichia coli for production of renewable ethylene glycol.

Biotechnol Bioeng 2016 Feb 18;113(2):376-83. Epub 2015 Sep 18.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139.

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http://doi.wiley.com/10.1002/bit.25717
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http://dx.doi.org/10.1002/bit.25717DOI Listing
February 2016

Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase.

ACS Chem Biol 2016 Feb 17;11(2):460-9. Epub 2015 Dec 17.

Department of Chemical Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts, United States.

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http://dx.doi.org/10.1021/acschembio.5b00767DOI Listing
February 2016

Engineering E. coli-E. coli cocultures for production of muconic acid from glycerol.

Microb Cell Fact 2015 Sep 15;14:134. Epub 2015 Sep 15.

Chemical Engineering Department, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.

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http://dx.doi.org/10.1186/s12934-015-0319-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4570557PMC
September 2015

Investigating Moorella thermoacetica metabolism with a genome-scale constraint-based metabolic model.

Integr Biol (Camb) 2015 Aug;7(8):869-82

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1039/c5ib00095eDOI Listing
August 2015

The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica.

Metab Eng 2015 Jul 6;30:27-39. Epub 2015 Mar 6.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2015.02.007DOI Listing
July 2015

Engineering Escherichia coli coculture systems for the production of biochemical products.

Proc Natl Acad Sci U S A 2015 Jul 25;112(27):8266-71. Epub 2015 Jun 25.

Chemical Engineering Department, Massachusetts Institute of Technology, Cambridge, MA 02139

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http://dx.doi.org/10.1073/pnas.1506781112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500268PMC
July 2015

13C isotope-assisted methods for quantifying glutamine metabolism in cancer cells.

Methods Enzymol 2014 ;542:369-89

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. Electronic address:

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http://dx.doi.org/10.1016/B978-0-12-416618-9.00019-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392845PMC
May 2015

Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production.

Appl Microbiol Biotechnol 2015 May 26;99(9):3825-37. Epub 2015 Feb 26.

Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore, Singapore.

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http://dx.doi.org/10.1007/s00253-015-6463-yDOI Listing
May 2015

Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica.

Metab Eng 2015 May 27;29:56-65. Epub 2015 Feb 27.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2015.02.005DOI Listing
May 2015

Distributing a metabolic pathway among a microbial consortium enhances production of natural products.

Nat Biotechnol 2015 Apr 5;33(4):377-83. Epub 2015 Jan 5.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://dx.doi.org/10.1038/nbt.3095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867547PMC
April 2015

Metabolomic and (13)C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase.

Biotechnol Bioeng 2015 Mar 24;112(3):470-83. Epub 2014 Nov 24.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, Massachussetts.

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http://dx.doi.org/10.1002/bit.25447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801006PMC
March 2015

Review of methods to probe single cell metabolism and bioenergetics.

Metab Eng 2015 Jan 31;27:115-135. Epub 2014 Oct 31.

Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, Cambridge, MA 02139, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ymben.2014.09.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399830PMC
January 2015

Pyruvate kinase isoform expression alters nucleotide synthesis to impact cell proliferation.

Mol Cell 2015 Jan 4;57(1):95-107. Epub 2014 Dec 4.

Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Dana-Farber Cancer Institute, Boston, MA 02115, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2014.10.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289430PMC
January 2015

Gregory Stephanopoulos and colleagues reply to Screening cellular metabolic activity.

Nat Biotechnol 2014 Nov;32(11):1092-3

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http://dx.doi.org/10.1038/nbt.3059DOI Listing
November 2014

Biofuels. Engineering alcohol tolerance in yeast.

Science 2014 Oct 2;346(6205):71-5. Epub 2014 Oct 2.

Department of Chemical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.

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http://dx.doi.org/10.1126/science.1257859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401034PMC
October 2014

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control.

Proc Natl Acad Sci U S A 2014 Aug 21;111(31):11299-304. Epub 2014 Jul 21.

Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180;Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180;Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180; and

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http://dx.doi.org/10.1073/pnas.1406401111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128127PMC
August 2014

Metabolic engineering: the ultimate paradigm for continuous pharmaceutical manufacturing.

ChemSusChem 2014 Jul 9;7(7):1847-53. Epub 2014 Apr 9.

Department of Chemistry&Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA 02138 (USA); Department of Chemical Engineering, Massachusetts Institute of Technology, 25 Ames St., Cambridge, MA 02139 (USA).

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http://dx.doi.org/10.1002/cssc.201301219DOI Listing
July 2014

Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption.

Nat Biotechnol 2014 May 6;32(5):473-8. Epub 2014 Apr 6.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://www.nature.com/articles/nbt.2857
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http://dx.doi.org/10.1038/nbt.2857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4412259PMC
May 2014

Fragment formula calculator (FFC): determination of chemical formulas for fragment ions in mass spectrometric data.

Anal Chem 2014 Feb 5;86(4):2221-8. Epub 2014 Feb 5.

Luxembourg Centre for Systems Biomedicine, University of Luxembourg , 7, avenue des Hauts-Fourneaux, L-4362 Esch-Belval, Luxembourg.

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http://dx.doi.org/10.1021/ac403879dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440337PMC
February 2014

c-Jun N-terminal kinase in synergistic neurite outgrowth in PC12 cells mediated through P90RSK.

BMC Neurosci 2013 Dec 12;14:153. Epub 2013 Dec 12.

Chemical & Pharmaceutical Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore, Singapore.

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http://dx.doi.org/10.1186/1471-2202-14-153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029309PMC
December 2013

Optimization of amorphadiene synthesis in bacillus subtilis via transcriptional, translational, and media modulation.

Biotechnol Bioeng 2013 Sep 7;110(9):2556-61. Epub 2013 Apr 7.

Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore.

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http://dx.doi.org/10.1002/bit.24900DOI Listing
September 2013

Metabolic engineering: past and future.

Annu Rev Chem Biomol Eng 2013 27;4:259-88. Epub 2013 Mar 27.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

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http://dx.doi.org/10.1146/annurev-chembioeng-061312-103312DOI Listing
September 2013

Kinetic isotope effects significantly influence intracellular metabolite (13) C labeling patterns and flux determination.

Biotechnol J 2013 Sep 5;8(9):1080-9. Epub 2013 Aug 5.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

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http://dx.doi.org/10.1002/biot.201200276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045492PMC
September 2013

NTFD--a stand-alone application for the non-targeted detection of stable isotope-labeled compounds in GC/MS data.

Bioinformatics 2013 May 11;29(9):1226-8. Epub 2013 Mar 11.

Luxembourg Centre for Systems Biomedicine, University of Luxembourg, L-4362 Esch-Belval, Luxembourg.

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http://dx.doi.org/10.1093/bioinformatics/btt119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634188PMC
May 2013

Cofactor balance by nicotinamide nucleotide transhydrogenase (NNT) coordinates reductive carboxylation and glucose catabolism in the tricarboxylic acid (TCA) cycle.

J Biol Chem 2013 May 15;288(18):12967-77. Epub 2013 Mar 15.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

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http://dx.doi.org/10.1074/jbc.M112.396796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3642339PMC
May 2013

Engineering E. coli for caffeic acid biosynthesis from renewable sugars.

Appl Microbiol Biotechnol 2013 Apr 21;97(8):3333-41. Epub 2012 Nov 21.

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Room 56-469, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1007/s00253-012-4544-8DOI Listing
April 2013

Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols.

Nat Biotechnol 2013 Apr 17;31(4):335-41. Epub 2013 Feb 17.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

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http://www.nature.com/articles/nbt.2509
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http://dx.doi.org/10.1038/nbt.2509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659820PMC
April 2013

Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production.

Metab Eng 2013 Jan 28;15:1-9. Epub 2012 Sep 28.

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

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http://dx.doi.org/10.1016/j.ymben.2012.08.007DOI Listing
January 2013

Loss of RBF1 changes glutamine catabolism.

Genes Dev 2013 Jan 15;27(2):182-96. Epub 2013 Jan 15.

Laboratory of Molecular Oncology, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, Massachusetts 02129, USA.

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http://dx.doi.org/10.1101/gad.206227.112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566311PMC
January 2013

Enhancing solubility of deoxyxylulose phosphate pathway enzymes for microbial isoprenoid production.

Microb Cell Fact 2012 Nov 14;11:148. Epub 2012 Nov 14.

Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore, Singapore.

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http://dx.doi.org/10.1186/1475-2859-11-148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545872PMC
November 2012

Incremental parameter estimation of kinetic metabolic network models.

BMC Syst Biol 2012 Nov 21;6:142. Epub 2012 Nov 21.

Chemical and Pharmaceutical Engineering, Singapore-MIT Alliance, Singapore 117576, Singapore.

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http://dx.doi.org/10.1186/1752-0509-6-142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568022PMC
November 2012

Synthetic biology and metabolic engineering.

ACS Synth Biol 2012 Nov;1(11):514-25

Department of Chemical Engineering, Massachusetts Institute of Technology, Building 56 Room 469C, 77 Massachusetts Ave, Cambridge, MA 02139, USA.

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http://dx.doi.org/10.1021/sb300094qDOI Listing
November 2012