Associate Professor  Khor Goot Heah , PhD - Universiti Teknologi MARA - Dr

Associate Professor Khor Goot Heah

PhD

Universiti Teknologi MARA

Dr

Shah Alam, Selangor | Malaysia

Main Specialties: Biology, Clinical & Laboratory Immunology, Dentistry, Medical Genetics, Oncology

Additional Specialties: Oncology, Molecular Biology

ORCID logohttps://orcid.org/0000-0002-4686-7668


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Associate Professor  Khor Goot Heah , PhD - Universiti Teknologi MARA - Dr

Associate Professor Khor Goot Heah

PhD

Introduction

Dr Khor is an Associate Professor in Centre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buloh Campus, Shah Alam, where she has been a Faculty member since 2006. She completed her studies in undergraduate of BioMedical Sciences, Master of Dental Science and PhD of Molecular Biology at University of Malaya, Malaysia. She holds the headship of Oral and Maxillofacial Cancer Research Group and associate member of Atta-ur Rahman Institute for Natural Product Discovery (AuRIns). She also actively involves as Faculty Research and Ethic committees. Her research interests are molecular biology, genetic and epigenetic studies on cancers, medicinal herbs, drug discovery, immunochemistry, molecular oncology and histopathological studies. She has collaborated actively with researchers in several other disciplines of medical, life, natural product and bioinformatics sciences, especially in cancer research. She awarded numerous national and international awards for intervention and innovative competitions. She has served as editorial board member and reviewers for numerous of national and international journals She also served as advisory panels and judges for several conferences and intervention and innovative competitions nationally and internationally. She can be contacted by email address: gootheah@uitm.edu.my or gootheah@gmail.com.

Primary Affiliation: Universiti Teknologi MARA - Shah Alam, Selangor , Malaysia

Specialties:

Additional Specialties:

Research Interests:


View Associate Professor Khor Goot Heah ’s Resume / CV

Education

Dec 2014
University of Malaya
PhD
Molecular Biology

Experience

Jan 2019
School of Medicine, Dentistry and Health SciencesUniversity of Melbourne
Visiting Scholar

Publications

17Publications

122Reads

49Profile Views

37PubMed Central Citations

Inhibitory Effect of 5-Aza-2-Deoxytidine on Cell Viability of the Oral Cancer Cell Line, ORL-48T.

International Journal of Engineering & Technology. 7:4(42):145-146

Khor GH, N.M.S Rawaidah, N.S Syairah, I. M. Amin, N. Harun, M.Y.P.M. Yusof,GRA Froemming,

...

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April 2020
8 Reads

Immunohistochemical detection of Def6 protein in oral cancer

Malaysian J Funda Appl Sci. 2019:30. 59-60

Malaysian Journal of Fundamental and Applied Sciences

Nowadays, oral cancer becomes global health issue and reaches 21st rank in Malaysian population. Def6 or IRF-4-binding proteins (IBP) that mostly produced in lymph node and thymus are associated with cell survival and cell proliferation. Def6 protein expression is often found in autoimmune diseases, but current studies also found Def6 in some cancers. In this study, we would like to detect Def6 protein expression in oral cancer. 36 formalin-fixed paraffin embedded tissues were selected for both test group (oral cancer) and control group (normal oral mucosa). The cases were retrieved from the archives of Faculty of Dentistry, University of Malaya, Kuala Lumpur. Immunohistochemistry (IHC) staining was manually performed using Def6 antibody (Abcam 1: 300) and assessed quantitatively (positivity and staining intensity). Positive and negative controls were used to validate the IHC run. All data were then analysed using SPSS version 25.0. Control group was negative for Def6 expression. 29 cases (82.86%) of oral cancer were Def6 positive with 2.86% weak staining, 5.71% moderate staining and 74.28% strong staining intensity. There was significant difference between Def6 expression in normal oral mucosa and oral cancer (p< 0.05). Thus, we postulate that Def6 expression plays a big role in carcinogenesis of human oral cancer.

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April 2020

1 Citation

15 Reads

A Review of MicroRNA Associated with Oral Cancer

J Int Dent Med Res. 2019.12 (2):738-743

Journal of International Dental and Medical Research

Cancer of oral cavity is the sixth most common malignancy worldwide with over 500,000 new cases diagnosed annually and its incidence is rising in many countries. It is associated with a poor prognosis with less than 50% of 5-year survival rate. Oral cancer incidence in Malaysia varies between the different ethnic communities due to different sociocultural risk factors such as the betel chewing habit which it is a traditional stimulant mixture of areca nut and tobacco with the betel leaf. Moreover, despite recent advances in various treatment modalities, the survival rates of cancer patients had shown not markedly improved.
MicroRNAs or miRNAs are a group of small non-coding RNAs that post-transcriptionally regulate gene expression. Recent findings have strongly supported its role in cell regulations of essential processes including cell proliferation, apoptosis, development, differentiation and metabolism. However, dysregulation of miRNAs expressions have been implicated in malignancy which affects its functions as tumor suppressors and oncogenes in various cancers. Thus, this review of microRNA expression would provide information on microRNAs with potential role as the biomarkers in oral cancer treatment in the future.

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April 2020
12 Reads

DEF6 Expression and Regulation in Cancer, Chronic Inflammatory Diseases and Autoimmune Diseases: A Review

Journal of International Dental and Medical Research

Def6 protein has been highly associated with autoimmune diseases and become one of risk gene in systemic lupus erythematosus (SLE) disease. Def6 deficiency is spontaneously affecting the development of systemic autoimmune disorder in mice as well as in human. Def6 or IRF-4binding proteins (IBP) that mostly found among lymph node and thymus are associated with cell survival and cell proliferation. Def6 protein as a novel biomarker has many important functions and roles in human immune system. Def6 is believed to be a potential therapeutic pharmacological target or/and potential drug for some malignancy diseases and pathological disorders. Current studies reveal Def6 expression and regulations not only are found in autoimmune diseases but also in human cancer and chronic inflammatory diseases. High expression of Def6 is correlated with invasive tumours and malignant stage of various cancers.  This review article sums up Def6 expression and regulation in some diseases stated above. Collectively, this review article provides novel studies concerning Def6 as a protein, its correlation with other genes, its mechanism and its expression in some diseases, particularly in cancer, autoimmune diseases and chronic inflammatory diseases

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April 2020
8 Reads

In Vitro and In Vivo Study of Ganoderma lucidum in Cancer.

Journal of International Dental and Medical Research

 Current cancer therapy such as chemotherapy and radiography are known to possess many side effects that could lead to serious complications. The application of natural product as complementary and alternative treatment provides significant advantages. It could increase the sensitivity of chemotherapy and radiography while at the same time reducing their associated side effects and complications. With regards to anticancer drugs, more than 50% of the modern oncological drugs are derived from natural products. An edible mushroom, Ganoderma lucidum has long been used in traditional and conventional medicine in China, for the prevention and treatment of various human diseases.       The fungus possesses a wide variety of bioactive compounds present in its fruiting bodies, mycelium and spores, including anticancer property that has been proven in in vitro and in vivo studies. The carcinostatic effects of G. lucidum have been shown in a variety of cancer cell lines, including breast, pancreas, lung, colon, skin and prostate. This paper presents in vitro and in vivo studies of the anticancer properties of G. lucidum in cancers. 

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April 2020
14 Reads

Primary stability of self-tapping dual etched implants.

Minerva Stomatol 2019 Dec;68(6):291-296

Center of Studies for Restorative Dentistry, Faculty of Dentistry, MARA Technology University, Jalan Hospital, Sungai Buloh, Malaysia -

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http://dx.doi.org/10.23736/S0026-4970.19.04242-0DOI Listing
December 2019

A simple method for in-house DNA polymerase purification for high-fidelity PCR amplification.

Iran J Microbiol 2019 Apr;11(2):181-186

Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6635313PMC
April 2019

A Review Of Ribonucleotide Reductase And Cancer Therapies

Journal of International Dental and Medical Research

Abstract: Mammalian Ribonucleotide reductase (RNR) provides the precursors needed for both DNA synthesis and repair process. Recent study shows RNR including RRM2 have been associated with various types of cancer and many studies imply that it plays biological roles in promoting cancer development. The important role plays by RNR, and its subunits RRM1, RRM2, and p53R2 in DNA synthesis and repair had found to be an attractive target for anticancer therapies. Usually targeting RNR in cancer treatment using known inhibitors, or new inhibitors that discovered using as molecular docking programs. This comprehensive strategy can be used to find new indications of clinically used drugs that failed during its development. Thus, this strategy has the advantages of cost reduction and bypasses the safety concerns by in silico evaluation of the inhibitor biological activity on the molecular target.

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September 2018

3 Citations

Impact Factor 0.220

14 Reads

Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma.

Asian Pac J Cancer Prev 2016 ;17(1):219-23

Centre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buluh Campus, Malaysia E-mail :

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http://dx.doi.org/10.7314/apjcp.2016.17.1.219DOI Listing
December 2016
14 Reads
1 Citation
1.500 Impact Factor

Human papilloma virus 18 detection in oral squamous cell carcinoma and potentially malignant lesions using saliva samples.

Asian Pac J Cancer Prev 2012 ;13(12):6109-13

Centre of Studies for Preclinical Science, Faculty of Dentistry, Universiti Teknologi MARA, Shah Alam, Malaysia.

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http://dx.doi.org/10.7314/apjcp.2012.13.12.6109DOI Listing
April 2016
6 Reads
6 Citations
1.500 Impact Factor

Overexpression of MMP13 is associated with clinical outcomes and poor prognosis in oral squamous cell carcinoma.

ScientificWorldJournal 2014 23;2014:897523. Epub 2014 Oct 23.

Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Oro-Maxillofacial Surgical and Medical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.1155/2014/897523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226172PMC
November 2015
41 Reads
9 Citations
1.220 Impact Factor

Impact of ellagic acid in bone formation after tooth extraction: an experimental study on diabetic rats.

ScientificWorldJournal 2014 18;2014:908098. Epub 2014 Nov 18.

Center of Preclinical Science, Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, Malaysia.

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http://dx.doi.org/10.1155/2014/908098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251085PMC
September 2015
16 Reads
3 Citations
1.220 Impact Factor

Screening of differential promoter hypermethylated genes in primary oral squamous cell carcinoma.

Asian Pac J Cancer Prev 2014 ;15(20):8957-61

Centre of Preclinical Science Studies, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia E-mail :

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http://dx.doi.org/10.7314/apjcp.2014.15.20.8957DOI Listing
July 2015
11 Reads
1.500 Impact Factor

DNA methylation profiling revealed promoter hypermethylation-induced silencing of p16, DDAH2 and DUSP1 in primary oral squamous cell carcinoma.

Int J Med Sci 2013 12;10(12):1727-39. Epub 2013 Oct 12.

1. Centre of Studies for Preclinical Science, Faculty of Dentistry, Universiti Teknologi MARA, Shah Alam. Selangor, Malaysia. ; 2. Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia. ; 3. Oral Cancer Research and Coordinating Center, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia. ; 8. Laboratory of Biomedical Science and Molecular Microbiology, UMBIO Cluster, Institute of Postgraduate, University of Malaya, Kuala Lumpur, Malaysia.

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http://dx.doi.org/10.7150/ijms.6884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805925PMC
May 2014
34 Reads
14 Citations
2.003 Impact Factor

P53 Expression as a marker of Microinvasion of Oral Squamous Cell Carcinoma.

8

Asian Pacific J Cancer Prev,

INTRODUCTION: Oral squamous cell carcinoma (OSCC) has high local recurrence, partly caused by the lack of clear margin identification on surgical removal of cancerous tissues. Direct visualization by immunostaining and fluorescent in situ hybridization (FISH) in tissue sections gives more definite information about genetic damage at margins with appropriately selected biomarkers. AIMS: To determine the usefulness of immunohistochemical techniques and FISH of the tumour suppressor TP 53 gene to identify microinvasion in marginal tissue sections and to relate the possible correlation between protein expression and genetic aberrations in OSCC cases in Malaysia. METHODS: Immunohistochemistry and FISH of TP 53 genes were applied on 26 OSCC formalin fixed paraffin embed (FFEP) blocks selected from two oral cancer referral centers in Malaysia. RESULTS: For p53 protein immunohistochemistry, 96% of the 26 OSCC studied showed positive immunostaining at the excision margins. In FISH assay, 48.9±9.7% of the cancerous cells were monoploid for p53 probe signals, 41.0±9.5 % were diploid, and 10.2±7.8 % were polyploid. A correlation between p53 immunostaining and TP53 gene aberrations was noted (p< 0.05). CONCLUSIONS: Immunohistochemical analysis of p53 protein expression and FISH of TP53 gene could be applied as screening tool for microinvasion of OSCC

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July 2011
16 Reads

A simple mouthwash method for genomic DNA isolation in molecular studies

Journal of Oral Oncology

Background: Application of PCR techniques requiring only minute amounts of Genomic DNA. Thus, a less invasive, simpler to perform, and cheaper method to obtain DNA from exfoliated cells is desirable. We aim to develop a method that can obtain high quality of genomic DNA from one sample that allows for numerous application of PCR analysis. Objective: This study describes a simple, inexpensive and non invasive protocol to isolate a high quality of genomic DNA from exfoliated cells by using swish method. Methodology: Twenty two subjects vigorously swished 10 ml of normal saline in their mouth for 60 s and spitted into a collection tube. DNA extraction assay was performed by using saliva DNA isolation kit (Norgen, USA). The washed pellets were suspended in TE buffer and analyzed for the quality and purity of DNA content by using the NanoDrop Spectrophotometer. A ratio of A260/A280 was calculated. The extracted genomic DNA was amplified with primers of p53 intron 6 by using PCR machine. The presence of amplified DNA was then confirmed by electrophoreses analysis, which DNA bands were scanned by Typhoon 9410 variable imager. Results: In this study, the extracted genomic DNA demonstrated an average value of 1.94 O.D. in DNA content purity and 42.9 \backslashensuremath\ensuremathμg/\backslashensuremath\ensuremathμl in DNA yields. The electrophoresis images of the DNA products showed visible and detectable bands of higher molecular weight DNA in all the samples. Conclusion: The results showed that the extracted genomic DNA from the exfoliated cells by applying the swish method, that provides substantially larger amounts and higher molecular weight of DNA for down-stream DNA identification application. In addition, all samples were successfully genotyped by PCR-based assays for p53 gene intron 6 regions, which confirmed that the quality of isolated DNA was reliable in supporting the PCR amplification for the molecular studies.

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March 2009
156 Reads

Primary stability of self-tapping dual etched implants.

Minerva Stomatologica

BACKGROUND: The aims of this study were to enumerate the primary implant stability quotient (ISQ) value of self-tapping dual etched implants and to explore the influence of parameters such as implant length, implant diameter, age, gender, implant location and osteotomy preparation on the ISQ value.METHODS: Retrospective data from clinical worksheets given to participants during two implant courses held between the periods of 2013 to 2014 were evaluated. A total of 61 implants were considered based on the inclusion criteria. The effects of parameters such as implant diameter, implant length, age, gender, implant location and osteotomy protocol on ISQ values were analyzed.RESULTS: Mean ISQ value for all implants was 67.21±9.13. Age of patients (P=0.016) and location of implants (P=0.041) had a significant linear relationship with the ISQ values. Within the age limit of the patients in this study, it was found that an increase in one year of patient’s age results in 0.20 decrease in ISQ value (95% CI: -0.36, -0.04). However, placing an implant in the posterior maxilla may negatively affect the ISQ with a likely decrease in primary stability by 6.76 ISQ value (95% CI: -13.22, -0.30).CONCLUSIONS: The results suggest that the mean ISQ achieved by the participants were comparable with the range reported for this particular type of implants. The patient’s age and location of implants were elucidated as the determinant factors of primary implant stability.KEY WORDS: Dental implants; Osseointegration; Osteotomy; Validation studies

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November -0001
11 Reads