Publications by authors named "Giuseppina Chiarenza"

3 Publications

  • Page 1 of 1

Seroprevalence of in Stray Dogs from Southern Italy.

Microorganisms 2020 Oct 30;8(11). Epub 2020 Oct 30.

Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", via G. Marinuzzi 3, 90129 Palermo, Italy.

is a bacterial pathogen transmitted by ticks and is responsible for Lyme disease in both humans and dogs. The aim of this work was to evaluate diffusion among stray dogs in Palermo (Sicily, Italy) by serological methods in order to study the risk factors associated with the infection. Serum and blood samples of 316 dogs were collected from a shelter in Palermo, and were analyzed for the presence of antibodies against by indirect immunofluorescence assay (IFA), and of the gene by real-time PCR, respectively. Seventeen sera (5.4%) were positive for the antibodies via IFA and one blood (0.3%) for via real time PCR. On the basis of serological results, the evaluation of the potential risk factors (sex, age, breed and coat color) was carried out. The multivariate analysis indicated that male sex is a factor significantly associated with seropositivity. This study confirms that male dogs have a higher risk of developing the disease than females, and represents the first investigation on the spread of among stray dogs in Sicily.
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http://dx.doi.org/10.3390/microorganisms8111688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692072PMC
October 2020

Seroprevalence of Coxiella burnetii in dairy cattle from Sicily.

Vet Ital 2019 Sep 20;55(3):247-252. Epub 2019 Sep 20.

Area Diagnostica Sierologica, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy. Tel.: +39 091 65 65 230, e-mail:

Q fever is a widespread zoonotic disease caused by Coxiella burnetii, an obligate intracellular bacterium with a wide range of hosts. The aim of this study was to estimate the seroprevalence of C. burnetii infection in cattle in Sicilian farms. A total of 4,661 serum samples, from cattle belonging to 198 Sicilian farms, were examined by ELISA test and 246 resulted positive. The average seroprevalence at the farm level was 38.8% (77/198) (95% CI), while at the animal level it was 5.28% (246/4,661) (95% CI). The present study highlights the need for continuous monitoring of C. burnetii spread as it represents a serious risk for human health.
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http://dx.doi.org/10.12834/VetIt.1391.7602.2DOI Listing
September 2019

Cross-reactivity in serological tests for brucellosis: a comparison of immune response of Escherichia coli O157:H7 and Yersinia enterocolitica O:9 vs Brucella spp.

Vet Ital 2018 Jun;54(2):107-114

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Campo Boario, 64100 Teramo, Italy.

According to European Union (EU) regulations, the serological tests for the eradication of bovine and ovine brucellosis are the Rose Bengal Test, Complement Fixation Test, and i-ELISA. These methods, also recommended by the World Organization for Animal Health (OIE) for international trades, have limitations related to the use of suspensions of smooth Brucellae or LPS extracts. Limitations include false-positive serological reactions to brucellosis, which in turn impedes accurate diagnosis in some herds. False positive reactions should be considered carefully during the final stages of an eradication programme and for surveillance purposes in brucellosis-free areas. In this study, we produced specific sera through the experimental infection of sheep with Y. enterocolitica O:9 and E. coli O157:H7. These are the most important cross-reactive bacteria with Brucella. We then evaluated the antibody response of groups of sheep that had been immunised towards homologous antigens and official antigens for brucellosis, in order to identify a differential diagnostic protocol to distinguish cross-reaction in Brucella-infected animals.
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http://dx.doi.org/10.12834/VetIt.1176.6539.2DOI Listing
June 2018
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