Publications by authors named "Gisele Alborghetti Nai"

50 Publications

Resistance training mitigates hepato-cardiac changes and muscle mitochondrial protein reductions in rats with diet-induced obesity.

Heliyon 2021 Nov 14;7(11):e08374. Epub 2021 Nov 14.

Department of Physiotherapy - School of Sciences and Technology - Sao Paulo, State University - UNESP, Campus Presidente Prudente, Brazil.

Aim: To investigate the effect of resistance training (RT) on hepatocardiovascular and muscle mitochondrial parameters in rats that were fed a high-calorie diet for 12 weeks.

Main Methods: The animals were divided into four groups: control (C), exercise (E), obese (O), and obese plus exercise (OE). Group E and OE rats performed resistance training by climbing on a vertical ladder with load attached to the end of the tail (1×/day, 3×/week, for 12 weeks). Group O and OE rats were fed a high-calorie diet containing chow and a cafeteria diet for 12 weeks. Under anesthesia, the heart and liver were removed for histopathological analysis, and the gastrocnemius muscle was removed for Western blotting.

Key Findings: Group O rats were heavier, with increased fat mass, elevated fasting glycemia, and total triglycerides, and exhibited a significant number of Kupffer cells and diffuse steatosis in the liver. Group O rats also showed increased thickness of the right ventricle, septum, and pulmonary artery. All of these parameters were attenuated by RT. PGC1-α protein levels were increased in both exercise groups. The protein levels of OXPHOS complexes III, IV, and V were reduced in Group O, while RT prevented this alteration.

Significance: RT exerts a protective effect against hepato-cardiac alterations and prevents changes in the muscle mitochondrial protein profile induced by a high-calorie diet.
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http://dx.doi.org/10.1016/j.heliyon.2021.e08374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605435PMC
November 2021

Fractal dimension analysis: A new tool for analyzing colony-forming units.

MethodsX 2021 12;8:101228. Epub 2021 Jan 12.

Department of Pathology, Botucatu Medical School, São Paulo State University (UNESP), Rubião Junior, s/n, 18618-970, Botucatu, SP, Brazil.

The gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis ( = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium.•Petri dishes with different bacterial colonies were photographed with a digital camera under natural light.•The images were binarized and analyzed with ImageJ's "fractal dimension" tool.•Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit.
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http://dx.doi.org/10.1016/j.mex.2021.101228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374233PMC
January 2021

Subchronic exposure to a glyphosate-based herbicide causes dysplasia in the digestive tract of Wistar rats.

Environ Sci Pollut Res Int 2021 Nov 26;28(43):61477-61496. Epub 2021 Jun 26.

Graduate Program in Animal Science, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, SP, Brazil.

Glyphosate-based herbicides (GBH) are the most widely used herbicide for treatment of crops in the world. The digestive tract is one of the first systems exposed to pesticides, and damage to this system can affect the general health of individuals. The aim of this study was to evaluate the effects of subchronic inhalation and oral exposure to GBH on the digestive tract in rats. Six groups of Wistar rats (male and female) were exposed to nebulization with three concentrations of GBH [3.71 × 10 grams of active ingredient per hectare (g.a.i./ha), 6.19 × 10 g.a.i./ha and 9.28 × 10 g.a.i./ha] administered orally or by inhalation for 75 days. Bone marrow cells, smears of the tongue and fragments of the tongue, oesophagus, stomach and intestine were collected for histopathological analysis. Congestion, inflammation, an increase in the number of mast cells and nucleoli-organizing regions were detected in the tongue in the groups exposed to GBH. Females had a higher number of mast cells in the tongue than males. Animals in the groups exposed to higher concentrations of GBH showed dysplasia in the oesophagus and small and large intestine regardless of sex. Gastric changes were not observed. Animals exposed to GBH showed increased micronucleus formation. Our data indicate that GBH causes oral allergies and dysplastic lesions in the oesophagus and small and large intestine and has genotoxic potential.
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http://dx.doi.org/10.1007/s11356-021-15051-6DOI Listing
November 2021

Digital image processing: a useful tool in the analysis of lung injuries caused by chronic inhalation of agricultural herbicides.

Environ Sci Pollut Res Int 2021 Nov 7;28(41):57918-57924. Epub 2021 Jun 7.

Environmental and Regional Development, Oeste Paulista University, Km 572, SP-270 - Bairro Limoeiro, Pres. Prudente, SP, 19026-310, Brazil.

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used in agriculture to control various weeds. The objective of this study was to use the digital image processing method to identify alveolar lesions in the lungs of rats submitted to chronic 2,4-dichlorophenoxyacetic acid (2,4-D) inhalation exposure. We used forty adult male Wistar rats. The rats were divided into four groups: control group (CG), low concentration group (LCG), medium concentration group (MCG), and high concentration group (HCG). In a 6-month exposure period, we used two boxes connected to ultrasonic nebulizers for herbicide spraying. After this period, the rats were euthanized for the collection and study of lung tissue. For each image, counts of injuries and blisters were performed automatically using a methodology based on digital image processing techniques. For analysis of the results, an electronic database (Excel®) was created. We used the Pearson method for correlation analysis; values of p <0.05 were considered significant. In the evaluation of healthy alveoli, we recorded positive and significant correlations between analysis from a pathologist and computational analysis. In the evaluation of injured alveoli, we recorded a positive but non-significant correlation between analysis from a pathologist and computational analysis. These results show the effectiveness of digital image processing when evaluating alveolar integrity.
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http://dx.doi.org/10.1007/s11356-021-14692-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183328PMC
November 2021

Does washing medical devices before and after use decrease bacterial contamination?: An in vitro study.

Medicine (Baltimore) 2021 Apr;100(13):e25285

Department of Pathology, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.

Abstract: Surface treatment of medical devices may be a way of avoiding the need for replacement of these devices and the comorbidities associated with infection. The aim of this study was to evaluate whether pre- and postcontamination washing of 2 prostheses with different textures can decrease bacterial contamination.The following microorganisms were evaluated: Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis and Enterococcus faecalis. Silicone and expanded polytetrafluoroethylene vascular prostheses were used and divided into 3 groups: prostheses contaminated; prostheses contaminated and treated before contamination; and prostheses contaminated and treated after contamination. Treatments were performed with antibiotic solution, chlorhexidine and lidocaine. After one week of incubation, the prostheses were sown in culture medium, which was incubated for 48 hours. The area of colony formation was evaluated by fractal dimension, an image analysis tool.The antibiotic solution inhibited the growth of S epidermidis and chlorhexidine decrease in 53% the colonization density for S aureus in for both prostheses in the pre-washing. In postcontamination washing, the antibiotic solution inhibited the growth of all bacteria evaluated; there was a 60% decrease in the colonization density of S aureus and absence of colonization for E faecalis with chlorhexidine; and lidocaine inhibited the growth of S aureus in both prostheses.Antibiotic solution showed the highest efficiency in inhibiting bacterial growth, especially for S epidermidis, in both washings. Lidocaine was able to reduce colonization by S aureus in post-contamination washing, showing that it can be used as an alternative adjuvant treatment in these cases.
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http://dx.doi.org/10.1097/MD.0000000000025285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021368PMC
April 2021

Cardiovascular damage associated with subchronic exposure to the glyphosate herbicide in Wistar rats.

Toxicol Ind Health 2021 Apr 24;37(4):210-218. Epub 2021 Feb 24.

Graduate Program in Animal Science, 70799Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, São Paulo, Brazil.

Glyphosate is the most widely used herbicide in the world. Although some studies have shown cardiac electrophysiological changes associated to glyphosate, the histopathological changes that this herbicide may cause in the cardiovascular system are not yet established. The aim of this study was to evaluate the cardiovascular effects of subchronic oral and inhalation exposure to the glyphosate herbicide in rats. Eighty albino Wistar rats were distributed into eight groups (five males and five females/group): inhalation control: nebulization with sodium chloride solution (NaCl); oral control: nebulized feed with NaCl; low inhalation concentration: nebulization with 3.71 × 10 grams of active ingredient per hectare (g.a.i./ha) of glyphosate; low oral concentration: nebulized feed with 3.71 × 10 g.a.i./ha of glyphosate; medium inhalation concentration: nebulization with 6.19 × 10 g.a.i./ha of glyphosate; medium oral concentration: nebulized feed with 6.19 × 10 g.a.i./ha of glyphosate; high inhalation concentration: nebulization with 9.28 × 10 g.a.i./ha of glyphosate; and high oral concentration: nebulized feed with 9.28 × 10 g.a.i./ha of glyphosate. After 75 days of exposure, the animals were euthanized, and aortas and hearts were collected for histopathological analysis. Fatty streaks were observed in most animals exposed to glyphosate and were more prevalent in male rats, regardless of the route of exposure ( < 0.05). There were no differences in the measurements of the thickness of the right and left ventricle or in the collagen density of both ventricles in any of the groups evaluated ( > 0.05). Our study suggests that glyphosate has atherogenic potential, regardless of the concentration and route of exposure.
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http://dx.doi.org/10.1177/0748233721996578DOI Listing
April 2021

Oral mucosa changes associated with chronic oral and inhalation exposure to 2,4-dichlorophenoxiacetic acid (2,4-D) in Wistar rats.

Toxicol Res (Camb) 2020 Dec 24;9(6):746-757. Epub 2020 Nov 24.

Department of Pathology, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, São Paulo 19050-680, Brazil.

The respiratory tract and the oral mucosa are the first areas contaminated by pesticides. The herbicide dichlorophenoxyacetic acid (2,4-D) is a widely used pesticide across the world for both crops and gardens. The aim of this study was to evaluate oral mucosal damage after an experimental simulation of chronic oral and inhalational environmental exposure to 2,4-D formulation. Eighty male Wistar rats were exposed to three distinct concentrations of 2,4-D formulation (low-187.17 mg/m; medium-313.31 mg/m; and high-467.93 mg/m). Oral exposure (through contaminated feed) or inhalation exposure lasted 6 months. Rat tongues were collected for cyto- and histopathology. There was a difference between exposure groups in the intensity of tissue congestion. Most rats exposed to 2,4-D presented mucosal inflammation at both cytology and histology ( < 0.05). Hyperkeratosis only occurred in rats exposed orally at the high concentration. There was an increase in the number of nucleoli-organizing regions in the dorsal epithelium as the 2,4-D concentration increased ( < 0.001). The inhalation route was more associated with increased mitosis figures and nucleoli-organizing region count ( < 0.05). Chronic oral and inhalation exposure to high concentrations of 2,4-D formulation caused an increase in the proliferation rate and thickness of the tongue epithelium and stimulated the inflammatory response in the tissue.
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http://dx.doi.org/10.1093/toxres/tfaa085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786168PMC
December 2020

Evaluation of genotoxicity after acute and chronic exposure to 2,4-dichlorophenoxyacetic acid herbicide (2,4-D) in rodents using machine learning algorithms.

J Toxicol Sci 2020 ;45(12):737-750

Department of Pathology and the Graduate Program in Animal Science, Universidade do Oeste Paulista (UNOESTE), Brazil.

2,4-Dichlorophenoxyacetic acid (2,4-D) is one of the most widely used herbicides in the world, but its mutagenic and carcinogenic potential is still controversial. We simulated environmental exposure to 2,4-D, with the objective of evaluating the genotoxic effect of acute and chronic exposure to 2,4-D in rodents. We also evaluated the performance of machine learning algorithms in detecting differences in exposure groups through recognition performed from genotoxic characteristics. In the acute phase, 88 Swiss mice were used, distributed in five groups and exposed to nebulizations at different time intervals (24, 48, 72 and 192 hr). In the chronic phase, 88 Wistar rats were used, distributed in two groups (inhaled and oral) and exposed for six months. Femoral bone marrow cells were collected for a micronucleus test and comet assay. Data were evaluated by pattern recognition algorithms. In acute exposure, medium and high concentrations induced DNA damage in the comet assay, but these concentrations did not increase micronucleated cells. In the chronic exposure, there was an increase in micronuclei and DNA damage in the comet assay in all exposed groups regardless of the exposure route. The data showed a robust pattern of distinction between exposed and nonexposed groups to 2,4-D. Our data showed that both acute inhalation exposure and chronic oral and inhalation exposure to 2,4-D can cause genotoxic effects regardless of concentration. Machine learning showed a clear distinction between the control groups and those exposed to 2,4-D, and the effects of exposure are not concentration-dependent.
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http://dx.doi.org/10.2131/jts.45.737DOI Listing
April 2021

Mycobacterium leprae on Palatine Tonsils and Adenoids of Asymptomatic Patients, Brazil.

Emerg Infect Dis 2020 10;26(10):2518-2520

We investigated palatine tonsil and adenoid specimens excised from otorhinolaryngological patients in a leprosy-endemic region of Brazil. Fite-Faraco staining identified Mycobacterium spp. in 9 of 397 specimen blocks. Immunohistochemistry and molecular analysis confirmed the presence of Mycobacterium leprae, indicating that these organs can house M. leprae in persons inhabiting a leprosy-endemic region.
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http://dx.doi.org/10.3201/eid2610.191267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510695PMC
October 2020

Effect of different doses of 2,4-dichlorophenoxyacetic acid (2,4-D) on cardiac parameters in male Wistar rats.

Environ Sci Pollut Res Int 2021 Jan 9;28(3):3078-3087. Epub 2020 Sep 9.

Postgraduate Animal Science Program, University of Western São Paulo (UNOESTE), Presidente Prudente, Brazil.

The objective of this study was to evaluate the effects of different doses of 2,4-dichlorophenoxyacetic herbicide in rat hearts. Exposure was through rat food that was nebulized with the herbicide. Thirty adult male Wistar rats (200-300 g) were used. The diet was exposed to 2,4-D in two different doses (CG: control group 10 ml distilled water; LCG: low concentration group 3.71 × 10 g.ia/ha diluted in 10 ml saline at 0.9% and HCG: High concentration group 9.28 × 10 g.ia/ha diluted in 10 ml 0.9% saline). After 6 months of exposure, blood samples were collected for CKMB evaluation, and left ventricular fragments were analyzed by histological evaluation, fibrosis measurements, fractal dimension and immunohistochemistry (BAX, Bcl2, TNF-α and NF-kB). There were no significant changes in CK-MB concentration, histological parameters, fibrosis measurements and fractal dimension. Long-term oral consumption of food nebulized by the herbicide 2,4-D promoted an increase in BAX, Bcl-2/BAX, and cytoplasmic NF-kB in the nuclear area of the group that received the highest dose of the herbicide. This suggests that the herbicide induces cardiotoxicity.
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http://dx.doi.org/10.1007/s11356-020-10699-yDOI Listing
January 2021

Resistance training prevents right ventricle hypertrophy in rats exposed to secondhand cigarette smoke.

PLoS One 2020 7;15(8):e0236988. Epub 2020 Aug 7.

Department of Physiotherapy, School of Sciences and Technology, Sao Paulo State University (UNESP), Presidente Prudente, São Paulo, Brazil.

Exposure to secondhand cigarette smoke is associated with the development of diverse diseases. Resistance training has been considered one of the most useful tools for patients with pulmonary disease, improving their quality of life. This study aimed to evaluate the effect of resistance training (RT) on the prevention of thickening of the right ventricle wall of rats exposed to secondhand cigarette smoke. Thirty-two Wistar rats were divided into four groups: Control (C), Smoker (S), Exercised (E) and Exercised Smoker (ES). The smoker groups were exposed to the smoke of four cigarettes for 30 min, twice daily, five days a week, for 16 weeks. The exercised groups climbed on a vertical ladder with progressive load, once a day, five days a week, for 16 weeks. The heart, trachea, lung, liver and gastrocnemius muscle were removed for histopathological analysis. Pulmonary emphysema (S and ES vs C and E, P < 0.0001) and pulmonary artery thickness enlargement (S vs C and E, P = 0.003, ES vs C, P = 0.003) were detected in the smoking groups. There was an increase in the right ventricle thickness in the S group compared with all other groups (P < 0.0001). An increase in resident macrophages in the liver was detected in both smoking groups compared with the C group (P = 0.002). Additionally, a relevant reduction of the diameter of the muscle fibers was detected only in ES compared with the C, S and E groups (P = 0.0002), impairing, at least in part, the muscle mass in exercised smoking rats. Therefore, it was concluded that resistance training prevented the increase of thickness of the right ventricle in rats exposed to secondhand cigarette smoke, but it may be not so beneficial for the skeletal muscle of smoking rats.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236988PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413484PMC
October 2020

Evaluation of buccal damage associated with acute inhalation exposure to 2,4-dichlorophenoxyacetic acid (2,4-D) in mice.

BMC Vet Res 2020 Jul 14;16(1):244. Epub 2020 Jul 14.

Department of Pathology, Graduate Program in Animal Science, Universidade do Oeste Paulista (UNOESTE), 19050-680, Presidente Prudente, SP, Brazil.

Background: The herbicide dichlorophenoxyacetic acid (2,4-D) is one of the most widely used crop spraying products in the world. Some pesticides induce the degranulation of mast cells and increase allergic responses. This is the first study to evaluate the damage to the oral mucosa after an experimental simulation of environmental inhalation exposure to the 2,4-D herbicide. The aim of this study was evaluate the possible oral damage caused by acute inhalation exposure to the herbicide 2,4-D.

Results: There was a difference between the exposure concentrations in relation to tissue congestion intensity (p = 0.002) and mast cell counts (p = 0.002), a difference in the evaluation of the interaction between the exposure concentrations and nebulization time in the dorsum epithelium thickness (p = 0.013), and a significant correlation between the epithelial thickness and the number of nucleoli organizing regions on the dorsum of the tongue (p = 0.048).

Conclusions: Even after acute exposure, the herbicide 2,4-D had the potential to damage the oral epithelium, especially at higher doses.
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http://dx.doi.org/10.1186/s12917-020-02461-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362426PMC
July 2020

Anetoderma: an alert for antiphospholipid antibody syndrome.

An Bras Dermatol 2020 Jan - Feb;95(1):123-125. Epub 2019 Dec 18.

Department of Pathology, Universidade do Oeste Paulista, Presidente Prudente, SP, Brazil.

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http://dx.doi.org/10.1016/j.abd.2019.04.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058882PMC
March 2020

Effects of consumption of contaminated feed with 2,4-dichlorophenoxyacetic acid (2,4-D) on the rat tibia: analysis by Raman spectroscopy and mechanical properties.

Lasers Med Sci 2020 Oct 17;35(8):1703-1709. Epub 2020 Jan 17.

Faculty of Nutrition, University of Western São Paulo (UNOESTE), São Paulo, Brazil.

Studies reported the harmful effects of 2,4-D on body tissues, provoking changes in the anatomy and physiology of the kidneys, liver, and testicles. Thus, the objective was to evaluate if there were alterations in the bone quality of the tibia of rats submitted to feed consumption that were exposed to three different 2,4-D doses. Male Wistar rats were divided into four groups: oral control group (C: feed consumption without 2,4-D contamination); low oral concentration group (G3: contaminated feed with low concentration of 2,4-D); medium concentration group (G6: contaminated feed with medium concentration of 2,4-D); and high concentration group (G9: contaminated feed with high concentration of 2,4-D). The results demonstrated alterations of the mechanical properties and Raman ratios of the tibias of the contaminated groups. The maximum load, maximum stress, elastic modulus, and the cortical area were lower in the G6 and G9 compared to C group. The mineral-to-matrix ratio (relative mineral to organic content) was lower in the G6 and G9 groups compared to C group, but carbonate-to-matrix ratio (indicator of bone turnover) was higher in both groups. Thus, it is possible to suggest that the 2,4-D herbicide performed deleterious effects on the bone quality of male Wistar rats.
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http://dx.doi.org/10.1007/s10103-020-02961-zDOI Listing
October 2020

Reduction in the inflammatory markers CD4, IL-1, IL-6 and TNFα in dogs with keratoconjunctivitis sicca treated topically with mesenchymal stem cells.

Stem Cell Res 2019 08 31;39:101525. Epub 2019 Jul 31.

Postgraduate Program in Animal Science, Universidade of Oeste Paulista (UNOESTE), Presidente Prudente, SP, Brazil. Electronic address:

Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellent model for understanding this disease, as the origin of KCS in dogs is like that in humans. The objective of this study was to localize and quantify immunological markers, such as CD4 lymphocytes, interleukin (IL)-1, IL-6 and tumor necrosis factor alpha (TNFα), before and after topical treatment with mesenchymal stem cells (MSCs). Twenty-two dogs positive for KCS were topically treated with 50 μL (1 × 10 MSCs) in the conjunctival sac and were evaluated for 6 months. The levels of the markers CD4, IL-6, IL-1 and TNFα were analyzed in conjunctival biopsy and cytology of the third eyelid gland by immunohistochemistry and immunocytochemistry. The results showed that before treatment, there was marked expression of all the markers (CD4, IL-6, IL-1 and TNFα), and after 6 months, there were significant (p < .05) reductions in the expression levels of all the markers. These results demonstrated that topical MSC treatment promotes a significant decrease in the expression levels of these inflammatory markers and could be used as adjuvant therapy in the treatment of KCS in dogs and humans. In addition, these markers can be excellent tools for diagnosing and analyzing the progression of KCS.
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http://dx.doi.org/10.1016/j.scr.2019.101525DOI Listing
August 2019

The Use of Platelet-Rich Plasma and Rosuvastatin in Wound Healing in Rabbits: A Longitudinal Study.

Adv Skin Wound Care 2019 Sep;32(9):1-5

At Oeste Paulista University, Presidente Prudente, in São Paulo, Brazil, Amanda Feba Tetila, MSc, is a physiotherapist and postgraduate student; Maria Rosa Santos Breda, MSc, is a veterinarian and postgraduate student; Rosa Maria Barilli Nogueira, PhD, is a Professor, Postgraduate Program in Animal Science; Gisele Alborghetti Nai, PhD, is a Professor, Postgraduate Program in Animal Science; and Cecília Braga Laposy, PhD, is a Professor, Postgraduate Program in Animal Science. Acknowledgment: Support for this article was provided by the Foundation for Research Support of the State of São Paulo (FAPESP, process no. 2016/25938-0). The authors have disclosed no other financial relationships related to this article. Submitted September 3, 2018; accepted in revised form November 26, 2018; published online ahead of print August 9, 2019.

Objective: To test the hypothesis that platelet-rich plasma (PRP) improves wound healing in rabbits by analyzing morphologic alterations in lesions with the use of PRP alone or combined with rosuvastatin (RSV).

Methods: Eight adult male New Zealand rabbits were used in this study. Researchers created four wounds on the back of each rabbit with an 8-mm punch. The control wound was treated with a 0.9% sodium chloride solution. Experimental wounds were treated with PRP, RSV, or both PRP and RSV. Dressings were changed with a new application of PRP and RSV every 4 days for 16 days. Wounds were biopsied on days 0, 7, 10, 14, and 17 for histopathologic evaluation of the scar tissue.

Main Results: Histopathology revealed reepithelialization in 100% of wounds treated with PRP alone after 17 days of treatment, compared with 50% of wounds treated with RSV alone and 75% of the wounds treated with PRP and RSV. Further, combining RSV and PRP reduced blood loss. The use of PRP alone induced 100% neovascularization, compared with 50% and 62.5% in wounds treated with PRP and RSV and RSV alone, respectively. All experimental wounds had a higher percentage of collagen fibers on day 17 postlesion when compared with control wounds (78.27% ± 4.69%). There were no significant differences among treatments; however, wounds treated with RSV alone had the lowest amount of collagen fibers (85.98% ± 3.51%). Wounds treated with PRP alone or PRP and RSV had 90.07% ± 6.20% and 90.76% ± 3.51% collagen fibers, respectively.

Conclusions: The results of this study indicate that PRP elicits higher healing activity in the first 7 days postlesion. Treatments with RSV alone or RSV and PRP did enhance other healing phases.
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http://dx.doi.org/10.1097/01.ASW.0000577136.88748.68DOI Listing
September 2019

Is dermoscopy a good tool for the diagnosis of lentigo maligna and lentigo maligna melanoma? A meta-analysis.

Clin Cosmet Investig Dermatol 2019 5;12:403-414. Epub 2019 Jun 5.

Medical School, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, SP, 19050-680, Brazil.

Dermoscopy is a low-cost examination performed by a dermatologist and good for the diagnosis of pigmented lesions. However, dermoscopy diagnosis of lentigo maligna (LM) and lentigo maligna melanoma (LMM) is still questionable. The objective of this study was to evaluate whether dermoscopy is an effective diagnostic method to diagnose LM/LMM from other pigmented skin lesions, and to identify which are the most frequent dermoscopic criteria associated with LM/LMM For this systematic review and meta-analysis, we used the following descriptors: dermoscopy, lentigo maligna, lentigo maligna melanoma, histopathology; and the following databases to search for articles: Cochrane Collaboration, MEDLINE; PMC (PubMed Central) - NIH (National Institutes of Health), EMBASE (The Excerpt Medical Database), and SCISEARCH, from inception to March 30, 2018. The evaluation of studies was performed using the QUADAS (Quality Assessment of Diagnostic Accuracy Studies)-2 tool. The PRISMA (Preferred Reporting Itens for Systematic Reviews and Meta-Analyses) and MOOSE (Meta-analysis Of Observational Studies in Epidemiology) guidelines were followed for data extraction. Also, we extracted from each study the dermoscopic criteria most commonly found in the lesions of LM/LMM. This systematic review included 15 articles for qualitative analysis (a total of 2,012 lesions evaluated) and 7 for meta-analysis. In the bivariate model the mean sensitivity was 0.824 and the mean specificity was 0.835. The area under the curve was 0.889. Rhomboid structures, pseudonetwork, and homogeneous areas were the most frequent dermoscopic criteria associated with LM/LMM. These findings suggest that dermoscopy has good accuracy in the diagnosis of LM/LMM.
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http://dx.doi.org/10.2147/CCID.S208717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6556474PMC
June 2019

Schistosomal Myeloradiculopathy - A case report.

Rev Soc Bras Med Trop 2019 May 16;52:e20180335. Epub 2019 May 16.

Faculdade de Medicina, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.

The most common neurological impairments related to schistosomiasis involve the lower portions of the medulla and the cauda equina. A 22-year-old woman, with no history, signs, or symptoms of hepatointestinal schistosomiasis, presented with lumbar pain associated with acute paresthesia and paresis of the right lower limb. Spinal schistosomiasis was suspected based on the disease progression and radiological findings, and the diagnosis was confirmed after cerebrospinal fluid analysis. The authors emphasize this pathology as important as a differential diagnosis in similar clinical scenarios, especially in endemic areas, because both early diagnosis and treatment are essential to avoid permanent sequelae.
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http://dx.doi.org/10.1590/0037-8682-0335-2018DOI Listing
May 2019

Dercum's disease: a rare and underdiagnosed disease.

An Bras Dermatol 2019 Mar-Apr;94(2):251-253. Epub 2019 May 9.

Dermatology Service, Hospital Regional de Presidente Prudente, Universidade do Oeste Paulista, Presidente Prudente (SP), Brazil.

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http://dx.doi.org/10.1590/abd1806-4841.20198231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6486061PMC
July 2019

Protective effects of on hepatoxicity and nephrotoxicity induced by carbon tetrachloride in rats.

Drug Chem Toxicol 2021 Jan 5;44(1):64-74. Epub 2018 Nov 5.

Faculty of Pharmacy, University of Western São Paulo (UNOESTE), Presidente Prudente, SP, Brazil.

The aim of this study was to assess the protective effects of oral and topical treatment with (BP) against carbon tetrachloride (CCl) induced toxicity. Fifty-six rats were divided into seven groups: A: CCl only; B: CCl+oral BP; C: CCl and topical BP; D: CCl+oral and topical BP; E: oral BP only; F: negative control; and G: positive control (cyclophosphamide). The animals were treated for 10 weeks. Blood samples were collected for tests of hepatic and renal function, and fragments of the liver, spleen, pancreas, kidney, and intestine were collected for histopathological analyses. Cells from the femoral bone marrow were used for a micronucleus test and 'comet assay'. Statistically significant differences were observed in the levels of gamma-glutamyl transpeptidase (GGT), albumin, urea and creatinine, hepatic inflammation, renal tubular lesion, and inflammation of the intestinal mucosa between the BP-treated groups and untreated group. The median number of micronuclei in group A was 4.00, in group G was 9.00 and in the other groups was 0.00. Group A had the lowest number of cells with a score of 0 and the greatest number with scores of 3 and 4, similar to the results obtained from group G using the 'comet assay'. Thus, BP effectively protected against the toxic effects of CCl on the liver, kidney, and intestine and exerted an antimutagenic effect on rats exposed to CCl.
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http://dx.doi.org/10.1080/01480545.2018.1526182DOI Listing
January 2021

Postpartum Genital Melanoma - A Case Report.

Rev Bras Ginecol Obstet 2018 Mar 23;40(3):163-167. Epub 2018 Feb 23.

Faculdade de Medicina, Universidade do Oeste Paulista, Presidente Prudente, São Paulo, Brazil.

Melanomas of the female genital tract may occur in the vulva, the vagina, the ovary or the cervix. Pregnancy has been considered an aggravating factor in the evolution and prognosis of melanoma. A 35-year-old female presented with vaginal bleeding 2 months after a term cesarean delivery. An endovaginal ultrasound revealed a lesion in the uterine cervix. The pathological report revealed a small round-cell neoplasm, and the immunohistochemistry confirmed the diagnosis of malignant melanoma. A positron emission tomography revealed an expansive hypermetabolic lesion centered on the cervix, and hypermetabolic lesions in the liver and right kidney. Non-surgical treatment was provided, with biochemotherapy followed by ipilimumab and nivolumab. The patient died one year later. Postpartum vaginal bleeding, even if late-onset, should be investigated, as it may be a pregnancy-associated malignant melanoma, which has a poor prognosis.
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http://dx.doi.org/10.1055/s-0038-1624578DOI Listing
March 2018

High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats.

Lasers Med Sci 2018 May 15;33(4):843-850. Epub 2018 Jan 15.

Department of Physical Therapy, University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil.

The aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm, output power 50 mW; energy density 69 J cm; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair.
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http://dx.doi.org/10.1007/s10103-018-2439-3DOI Listing
May 2018

Kissing nevus of the penis: a case report and dermatoscopic findings.

An Bras Dermatol 2017 ;92(5 Suppl 1):95-97

Department of Dermatology, Faculdade de Medicina da Universidade do Oeste Paulista (Unoeste) - Presidente Prudente - SP, Brazil.

Divided nevus, also known as kissing nevus, is a rare variant of congenital melanocytic nevi in which there are two adjacent nevi in areas of the body that undergo embryonic cleavage. The original description of this type of lesion was on the eyelid. The location on the penis is even rarer, with only 17 case reports in the literature so far, and only one of them described the dermoscopic findings. We report the case of a patient with divided nevus of the penis and its clinical, dermoscopic and histopathological features.
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http://dx.doi.org/10.1590/abd1806-4841.20175574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5726690PMC
March 2018

Congenital plaque-like glomangioma: report of two cases.

An Bras Dermatol 2017 ;92(5 Suppl 1):43-46

Department of Dermatology, Faculdade de Medicina da Universidade do Oeste Paulista (Unoeste) - Presidente Prudente (SP), Brazil.

Glomus tumors are rare hamartomas that originate from the glomus body. These tumors can be divided into solitary and multiple, the latter also known as glomangioma. We report the case of two patients with a rare variety of glomangioma called congenital plaque-like glomangioma. It presents as numerous red to bluish compressible papules, that increase in size in proportion with the weight and height growth of the child. Diagnostic confirmation is with histopathology and the treatment is surgical.
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http://dx.doi.org/10.1590/abd1806-4841.20175766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5726674PMC
March 2018

Evaluation of genotoxic effects in Brazilian agricultural workers exposed to pesticides and cigarette smoke using machine-learning algorithms.

Environ Sci Pollut Res Int 2018 Jan 30;25(2):1259-1269. Epub 2017 Oct 30.

Graduate Program in Environment and Regional Development, University of Western São Paulo - UNOESTE, Presidente Prudente, SP, Brazil.

Monitoring exposure to xenobiotics by biomarker analyses, such as a micronucleus assay, is extremely important for the precocious detection and prevention of diseases, such as oral cancer. The aim of this study was to evaluate genotoxic effects in rural workers who were exposed to cigarette smoke and/or pesticides and to identify possible classification patterns in the exposure groups. The sample included 120 participants of both sexes aged between 18 and 39, who were divided into the following four groups: control group (CG), smoking group (SG), pesticide group (PG), and smoking + pesticide group (SPG). Their oral mucosa cells were stained with Giemsa for cytogenetic analysis. The total numbers of nuclear abnormalities (CG = 27.16 ± 14.32, SG = 118.23 ± 74.78, PG = 184.23 ± 52.31, and SPG = 191.53 ± 66.94) and micronuclei (CG = 1.46 ± 1.40, SG = 12.20 ± 10.79, PG = 21.60 ± 8.24, and SPG = 20.26 ± 12.76) were higher (p < 0.05) in the three exposed groups compared to the GC. In this study, we considered several different classification algorithms (the artificial neural network, K-nearest neighbors, support vector machine, and optimum path forest). All of the algorithms displayed good classification (accuracy > 80%) when using dataset2 (without the redundant exposure type SPG). It is clear that the data form a robust pattern and that classifiers could be successfully trained on small datasets from the exposure groups. In conclusion, exposing agricultural workers to pesticides and/or tobacco had genotoxic potential, but concomitant exposure to xenobiotics did not lead to additive or potentiating effects.
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http://dx.doi.org/10.1007/s11356-017-0496-yDOI Listing
January 2018

Dermatologic manifestations of infective endocarditis.

An Bras Dermatol 2016 Sep-Oct;91(5 suppl 1):92-94

Universidade do Oeste Paulista (UNOESTE) - Presidente Prudente (SP), Brazil.

Despite advances in diagnosis and treatment, infective endocarditis still shows considerable morbidity and mortality rates. The dermatological examination in patients with suspected infective endocarditis may prove very useful, as it might reveal suggestive abnormalities of this disease, such as Osler's nodes and Janeway lesions. Osler's nodes are painful, purple nodular lesions, usually found on the tips of fingers and toes. Janeway lesions, in turn, are painless erythematous macules that usually affect palms and soles. We report a case of infective endocarditis and highlight the importance of skin examination as a very important element in the presumptive diagnosis of infective endocarditis.
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http://dx.doi.org/10.1590/abd1806-4841.20164718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325006PMC
July 2017

Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement.

Braz Oral Res 2016 Aug 18;30(1). Epub 2016 Aug 18.

Universidade do Oeste Paulista (UNOESTE), Dental School of Presidente Prudente, Presidente Prudente, SP, Brazil.

Ca3SiO5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca3SiO5in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca3SiO5-based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca3SiO5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca3SiO5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca3SiO5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca3SiO5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca3SiO5-based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.
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http://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0097DOI Listing
August 2016

Comparison of 1% cyclosporine eye drops in olive oil and in linseed oil to treat experimentally-induced keratoconjunctivitis sicca in rabbits.

Arq Bras Oftalmol 2015 Sep-Oct;78(5):295-9

Program in Animal Science, Universidade do Oeste Paulista, Presidente Prudente, SP, BR.

Purpose: To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two vehicles-olive and linseed oil-and that of the oils themselves in treating experimentally-induced keratoconjunctivitis sicca (KCS) in rabbits.

Methods: KCS was induced in 25 New Zealand rabbits using 1% atropine sulfate eye drops for 7 days before treatment and throughout the treatment period (12 weeks). The rabbits were divided into five groups: one control (C) group without KCS induction and four treatment groups in which KCS was induced and treated topically with olive oil (O), linseed oil (L), cyclosporine in olive oil (CO), and cyclosporine in linseed oil (CL). The animals were evaluated using Schirmer tear test 1 (STT), the fluorescein test (FT), tear-film break-up time (TBUT), the rose bengal test (RBT), and histopathological analysis.

Results: Values of STT and TBUT significantly decreased 1 week post-induction (p<0.05) and were similar to initial values after the 4th week of treatment, in all groups. After KCS induction, there was significantly less corneal damage in group L than in group CL, as assessed FT and RBT. Histopathology demonstrated that Groups L and CL presented less edema and corneal congestion. There was no significant difference in the goblet cell density (cells/mm2) between the groups (p=0.147).

Conclusion: Cyclosporine diluted in olive oil or linseed oil was effective in the treatment of KCS, although it had better efficacy when diluted in linseed oil. Linseed oil presented better effectiveness, whether associated or not, than olive oil. These results may contribute to the creation of novel topical ophthalmic formulations for KCS treatment in future.
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http://dx.doi.org/10.5935/0004-2749.20150078DOI Listing
April 2016

Study of the influence of the ph of water in the initiation of digestive tract injury in cadmium poisoning in rats.

Toxicol Rep 2015 19;2:1033-1038. Epub 2015 Jul 19.

Medical College, Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700, 19050-680 Presidente Prudente, SP, Brazil.

Cancer has genetic and environmental causes, one of which is the ingestion of heavy metals such as cadmium.

Objective: To evaluate the lesions caused by cadmium poisoning in the digestive tract and the possible effect of the drinking water pH in the initiation of these lesions.

Methods: 90 male Wistar rats were used, divided into six groups (= 15): A - received 400 mg/l cadmium chloride (CdCl2) in drinking water at a neutral pH of 7.0; B - received CdCl2 (400 mg/l) in drinking water at an acidic pH of 5.0; C - received CdCl2 (400 mg/l) in drinking water at a basic pH of 8.0; D - received water at an acidic pH of 5.0; E - received water at a basic pH of 8.0; and F - received water at a neutral pH of 7.0. Animals were euthanized after 6 months. Samples of the esophagus, stomach, small intestine and large intestine of each rat were removed for microscopic analysis.

Results: There were no microscopic changes in either the esophagus or small and large intestines. Only cadmium-exposed animals showed mild dysplasia of the gastric mucosa (= 0.012), regardless of the pH (> 0.05).

Conclusion: Cadmium exposure led to the formation of dysplastic lesions in the gastric glandular epithelium, regardless of the water pH.
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http://dx.doi.org/10.1016/j.toxrep.2015.07.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598283PMC
July 2015

Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits.

Acta Cir Bras 2015 Mar;30(3):209-15

Department of Medical Clinic, UNOESTE, Presidente Prudente, SP, Brazil.

Purpose: To evaluate the potential of heterologous platelet-rich plasma (PRP) gel for surgical skin wound healing in rabbits.

Methods: Blood from a single healthy dog was used for PRP production, with calcium gluconate added to the PRP to form the gel. Two surgical excisions, one to the right and the other to the left of the dorsal midline, were made in six rabbits. One side was randomly allocated to topical application of a physiological solution, and the other was allocated to treatment with heterologous PRP gel. Clinical assessments (weight, pain sensitivity, coloring, edema, hyperemia, exudation, crust, and granulation) and morphometric evaluations were performed 0, 3, 7, 10, 14, and 17 days postoperatively. Histological analysis was performed on the 17th day.

Results: With the exception of the presence of a crust at day 10, clinical variables did not differ significantly between the experimental groups. In both the control and PRP-treated groups, differences were identified when comparing time-points in terms of wound area reduction. Histological results indicated no significant differences between the control group and the PRP-treated group.

Conclusion: Heterologous platelet-rich plasma gel promoted dermal wound healing in rabbits with no adverse effects.
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http://dx.doi.org/10.1590/S0102-865020150030000008DOI Listing
March 2015
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