Publications by authors named "Gholamreza Dehghannoudeh"

8 Publications

  • Page 1 of 1

Development of a new electrochemical DNA biosensor based on Eu-doped NiO for determination of amsacrine as an anti-cancer drug: Electrochemical, spectroscopic and docking studies.

Anal Chim Acta 2020 Oct 12;1133:48-57. Epub 2020 Aug 12.

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; Environmental Health Engineering Research Center, Kerman University of Medical Sciences, Kerman, Iran. Electronic address:

The present research reported a new electrochemical biosensor based on ds-DNA/Eu doped NiO/CPE to detect amsacrine. Therefore, UV-Vis spectrophotometry, docking, and differential pulse voltammetry (DPV) have been used to study the interactions between amsacrine and dsDNA. Then, experimental parameters affected DNA immobilization and interactions between amsacrine and ds-DNA have been optimized. Afterwards, guanine oxidation peak current of ds-DNA has been chosen as a signal to analyze amsacrine in a concentration ranging between 0.1 and 100.0 μM and finally, limit of detection (LOD) of 0.05 μM has been calculated at optimal condition. Ultimately, it was found that the suggested biosensor is able to determine amsacrine in human serum and urine samples successfully.
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http://dx.doi.org/10.1016/j.aca.2020.07.071DOI Listing
October 2020

Potential Use of Microbial Surfactant in Microemulsion Drug Delivery System: A Systematic Review.

Drug Des Devel Ther 2020 5;14:541-550. Epub 2020 Feb 5.

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Background: Microemulsions drug delivery systems (MDDS) have been known to increase the bioavailability of hydrophobic drugs. The main challenge of the MDDS is the development of an effective and safe system for drug carriage and delivery. Biosurfactants are preferred surface-active molecules because of their lower toxicity and safe characteristics when compared to synthetic surfactants. Glycolipid and lipopeptide are the most common biosurfactants that were tested for MDDS. The main goal of the present systematic review was to estimate the available evidence on the role of biosurfactant in the development of MDDS.

Search Strategy: Literature searches involved the main scientific databases and were focused on the period from 2005 until 2017. The Search filter composed of two items: "Biosurfactant" and/or "Microemulsion."

Inclusion Criteria: Twenty-four studies evaluating the use of biosurfactant in MDDS were eligible for inclusion. Among these 14 were related to the use of glycolipid biosurfactants in the MDDS formulations, while four reported using lipopeptide biosurfactants and six other related review articles.

Results: According to the output study parameters, biosurfactants acted as active stabilizers, hydrophilic or hydrophobic linkers and safety carriers in MDDS, and among them glycolipid biosurfactants had the most application in MDDS formulations.

Conclusion: Synthetic surfactants could be replaced by biosurfactants as an effective bio-source for MDDS due to their excellent self-assembling and emulsifying activity properties.
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http://dx.doi.org/10.2147/DDDT.S232325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008186PMC
October 2020

Chitosan-based advanced materials for docetaxel and paclitaxel delivery: Recent advances and future directions in cancer theranostics.

Int J Biol Macromol 2020 Feb 20;145:282-300. Epub 2019 Dec 20.

Enhanced Composites and Structures Center, School of Aerospace, Transport and Manufacturing, Cranfield University, Bedfordshire MK43 0AL, UK; Department of Mechanical Engineering, School of Engineering, Shiv Nadar University, Uttar Pradesh 201314, India. Electronic address:

Paclitaxel (PTX) and docetaxel (DTX) are key members of taxanes with high anti-tumor activity against various cancer cells. These chemotherapeutic agents suffer from a number of drawbacks and it seems that low solubility in water is the most important one. Although much effort has been made in improving the bioavailability of PTX and DTX, the low bioavailability and minimal accumulation at tumor sites are still the challenges faced in PTX and DTX therapy. As a consequence, bio-based nanoparticles (NPs) have attracted much attention due to unique properties. Among them, chitosan (CS) is of interest due to its great biocompatibility. CS is a positively charged polysaccharide with the capability of interaction with negatively charged biomolecules. Besides, it can be processed into the sheet, micro/nano-particles, scaffold, and is dissolvable in mildly acidic pH similar to the pH of the tumor microenvironment. Keeping in mind the different applications of CS in the preparation of nanocarriers for delivery of PTX and DTX, in the present review, we demonstrate that how CS functionalized-nanocarriers and CS modification can be beneficial in enhancing the bioavailability of PTX and DTX, targeted delivery at tumor site, image-guided delivery and co-delivery with other anti-tumor drugs or genes.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.12.145DOI Listing
February 2020

Antimicrobial, anti-biofilm, and anti-proliferative activities of lipopeptide biosurfactant produced by Acinetobacter junii B6.

Microb Pathog 2020 Jan 17;138:103806. Epub 2019 Oct 17.

Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Lipopeptide biosurfactants (LPBs) are amphiphilic compounds produced by microorganisms exhibiting various biological activities. The main aim of the present study was to assess the in vitro antimicrobial, anti-biofilm, and cytotoxic effects of LPB produced by Acinetobacter junii (AjL). We determined AjL minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative bacteria as well as two fungal strains. Also, the anti-biofilm activity of AjL against the biofilm produced by clinically isolated bacterial strains was investigated. The AjL non-selectively showed activity against both Gram-positive and Gram-negative bacterial strains. The obtained results of the present study exhibited that the AjL in concentrations nearly below critical micelle concentration (CMC) has an effective antibacterial activity. It was found that the MIC values of AjL were lower than standard antifungal and it exhibited nearly 100% inhibition against Candida utilis. The attained results of the biofilm formation revealed that AjL disrupted the biofilm of Proteus mirabilis, Staphylococcus aureus, and Pseudomonas aeruginosa at 1250 μg/ml and 2500 μg/ml concentrations. The attained results of cytotoxic effect (determined by WST-1 assay) of the AjL revealed IC of 7.8 ± 0.4 mg/ml, 2.4 ± 0.5 mg/ml, and 5.7 ± 0.1 mg/ml, against U87, KB, and HUVEC cell lines, respectively. The results indicated that AjL has a potential application in the relatively new field of biomedicine.
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http://dx.doi.org/10.1016/j.micpath.2019.103806DOI Listing
January 2020

Hydrogels For Peptide Hormones Delivery: Therapeutic And Tissue Engineering Applications.

Drug Des Devel Ther 2019 26;13:3405-3418. Epub 2019 Sep 26.

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Peptides are the most abundant biological compounds in the cells that act as enzymes, hormones, structural element, and antibodies. Mostly, peptides have problems to move across the cells because of their size and poor cellular penetration. Therefore, a carrier that could transfer peptides into cells is ideal and would be effective for disease treatment. Until now, plenty of polymers, e.g., polysaccharides, polypeptides, and lipids were used in drug delivery. Hydrogels made from polysaccharides showed significant development in targeted delivery of peptide hormones because of their natural characteristics such as networks, pore sizes, sustainability, and response to external stimuli. The main aim of the present review was therefore, to gather the important usages of the hydrogels as a carrier in peptide hormone delivery and their application in tissue engineering and regenerative medicine.
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http://dx.doi.org/10.2147/DDDT.S217211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770672PMC
April 2020

Investigation of the structural, physicochemical properties, and aggregation behavior of lipopeptide biosurfactant produced by Acinetobacter junii B6.

Int J Biol Macromol 2018 Jun 6;112:712-719. Epub 2018 Feb 6.

Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.

In the present study the produced biosurfactant of Acinetobacter junii B6 (recently isolated from Iranian oil excavation site) were partially purified and identified by high performance thin layer chromatography (HPTLC), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (H NMR). Elemental analysis of the biosurfactant by energy dispersive X-ray spectroscopy (EDS) revealed that the biosurfactant was anionic in nature. The physiochemical properties of the lipopeptide biosurfactant were evaluated by determination of its critical micelle concentration (CMC) and hydrophile-lipophile balance (HLB). The produced biosurfactant decreased the surface tension of water to 36mNm with the CMC of approximately 300mg/l. Furthermore, the solubility properties of the biosurfactant (dissolved in phosphate-buffer saline solution, pH7.4) were investigated by turbidity examination, dynamic light scattering (DLS) measurements, and transmission electron microscopy (TEM) inspection. It could be concluded that the biosurfactant showed the spherical-shaped vesicles at a concentration higher than its CMC and the circular dichroism (CD) spectra showed that the secondary structure of the biosurfactant vesicles is dominated by the β sheet.
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http://dx.doi.org/10.1016/j.ijbiomac.2018.01.209DOI Listing
June 2018

Antioxidant Potential and Wound Healing Activity of Biosurfactant Produced by Acinetobacter junii B6.

Curr Pharm Biotechnol 2017 ;18(11):900-908

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Background: Recently, the development of a safe bioactive material with antioxidant properties, which can improve healing activity are focusing. Biosurfactants are very famous for their antimicrobial and free radical scavenging activities. Thereof, the main aim of the present study was to investigate the antioxidant and wound healing activity of the lipopeptide biosurfactant (LBS) produced by Acinetobacter junii B6. DPPH radical scavenging activities and FRAP assays were used to measure the antioxidant properties.

Methods: For evaluation of the wound healing activity, 36 rats (previously wounded in depilated thoracic region) were randomly distributed into six groups and chromatic, wound contraction, and histopathological feature were examined. The assessment levels of reactive oxygen species (ROS) after LBS exposure were determined using malondialdehyde (MDA), hydrogen peroxide (H2O2), and glutathione (GSH) assay kits.

Results: DPPH assay showed notable scavenging activities at the corresponding concentrations with IC50 value of 0.7 mg/ml. The reductive potency of the LBS showed lower performance at low concentration, while exhibited a remarkable increase at higher concentration. The best histopathological remission was achieved following treatment by 5 mg/ml of the LBS. Scar wounds at day 13 showed the lowest lesion sizes, increased re-epithelialization, hair follicle detection, and decreased amounts of neutrophilic inflammation, immaturity of the wound bed, erythema, edema, capillary, and retention of necrotic tissue.

Conclusion: Results from MDA, H2O2, and GSH levels of the treated sample confirmed the scavenging property of the bacterial derived LBS through ROS. It could be concluded that the pharmaceutical formula encourages the wound healing because of its notable antioxidant capacity.

Highlights: • DPPH and FRAP assays showed notable scavenging activity. • MDA, H2O2, and GSH; confirmed the scavenging property of the derived biosurfactant through ROS. • Synthesized formula encourages the healing of the wound because of its antioxidant capacity.
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http://dx.doi.org/10.2174/1389201018666171122121350DOI Listing
June 2018

Evaluation the thermodynamic behavior of nonionic polyoxyethylene surfactants against temperature changes.

Pak J Pharm Sci 2016 Mar;29(2):521-7

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Micellization is the most important property of surface agents. It plays an important role in the manufacture of pharmaceutical products. The surfactants have many applications in industry, agriculture, mining and oil recovery with functional properties as wetting, foaming and emulsifier in pharmaceutical and cosmetic products. The micellization parameters of surfactants help the manufacture of pharmaceutical products to be appropriate and stable. Therefore, in this study, Polyoxyethylene lauryl ether (C12E23), Polyoxyethylene (10) cetyl ether (C16E10) and Polyoxyethylene (20) cetyl ether (C16E20) were chosen as the nonionic surfactants to examine the effect of temperature variation (10-80(°)C) on the Critical Micelle Concentration (CMC). The measurement of surface tension was done by a Du Nöuys ring method. The value of CMC was obtained from the surface tension vs. surfactant concentration curve. Since the temperature was increased, the CMC initially decreased and then increased for each surfactant because the formation of the hydrogen bond is harder in the high temperatures. The surface tension γCMC for all three surfactant solutions decreased monotonically as the temperature increased. δG(°)m, ΔH(°)m and ΔS°m as the thermodynamic parameters of micellization, were also estimated and analyzed. The ΔG(°)m was decreased (10-80(°)C) if the temperature was increased. The entropy and enthalpy correlation of micellization showed a significant linearity. For C12E23, C16E20 and C16 E10, the compensation temperature (Tc) was obtained 309.5, 313.2 and 314.4 K, respectively. The calculated thermodynamic parameters showed that the entropy influenced on the micellization process at lower temperature, but it affected by enthalpy when temperature was increased.
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March 2016