Publications by authors named "Ghasem Farjanikish"

8 Publications

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The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit.

J Orthop Surg Res 2021 Jun 7;16(1):362. Epub 2021 Jun 7.

Department of Veterinary Surgery and Radiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Background: Bone fractures are medical emergencies that require prompt intervention to help return bone to its normal function. Various methods and treatments have been utilized to increase the speed and efficiency of bone repair. This study aimed to investigate the treatment effects of Prunus dulcis aqueous extract on tibial bone healing in rabbits.

Methods: All animals were distributed in five groups with six rats in each group, including the sham group, the control group in which tibial lesion was made and received distilled water, treatment groups with 150 mg kg, 300 mg kg doses of Prunus dulcis extract, and osteocare treated group. Biochemical blood factors including calcium, phosphorus, and alkaline phosphatase (on days 0, 10, 30, and 50), biomarkers of oxidative stress such as GPx, CAT, and MDA (on days 10 and 30), radiological evaluation, histopathological parameters, and osteocalcin immunohistochemical expression were assessed.

Results: The data showed calcium levels in the treatment groups increased significantly from day 10 to day 50, respectively, and blood phosphorus levels decreased from day 10 to day 50 in the treatment groups. Alkaline phosphatase initially increased and then decreased in treatment groups. In the treatment groups, GPx and CAT levels significantly increased, and the serum amount of MDA reduced. The best antioxidant results were related to the extract-treated group with a higher dose. Radiographic score was significantly higher in the treatment groups than the control group on day 30. Based on the pathological findings, the healing occurred faster in the extract-treated group with a higher dose. Osteocalcin expression was significantly higher in the control group than that in the treatment groups.

Conclusions: Treatment with Prunus dulcis extract with a dosage of 300 mg/kg accelerated tibial bone healing in rabbits.
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http://dx.doi.org/10.1186/s13018-021-02498-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183070PMC
June 2021

Nitroglycerin ameliorates sperm parameters, oxidative stress and testicular injury following by testicular torsion/detorsion in male rats.

Exp Mol Pathol 2020 12 2;117:104563. Epub 2020 Nov 2.

Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.

Purpose: This study was designed to determine the probable protection mechanisms of nitroglycerin, a widely used medication for treatment of heart failure and angina, in amelioration of testicular ischemia/reperfusion damage.

Methods: 24 adult male rats were randomly divided into three equal groups; with eight rats in each group: Group 1 (Sham) was sham-operated. Group 2 (T_D): 2 h testicular torsion was induced, afterward detorsion was performed and maintained for 2 h. Group 3 (NG): Nitroglycerin was administered immediately after detorsion. Sperm quality parameters such as viability, motility, morphology, and concentration, levels of antioxidant enzymes (glutathione peroxidase (GPX), catalase (CAT), and total antioxidant capacity (TAC)), and amount of malondialdehyde (MDA) in the blood plasma were examined in each group, thereafter histopathological parameters including germinal epithelial cell thickness (GECT), mean seminiferous tubular diameter (MSTD), Johnson's score and Cosentino's score were assessed.

Results: Testicular T_D significantly reduced sperm viability, motility, and normal morphology, whereas the NG administration remarkably increased the percentage of live, motile, and normal spermatozoa (p < 0.05). Levels of GPx, CAT, and TAC significantly reduced and the MDA level significantly increased in the T_D group in comparison to the sham group (p < 0.05). The NG treated group demonstrated significantly reduced MDA concentrations as well as elevated levels of GPx and CAT compared to the T_D group (p < 0.05). Induction of testicular torsion significantly reduced Johnson's score, GESCT (μm), and MSTD (μm), and remarkably increased the Cosentino's score (P < 0.05), while NG injection significantly increased Johnson's score, GESCT (μm), and MSTD (μm) and reduced the Cosentino's score (P < 0.05).

Conclusion: According to the findings in this research, nitroglycerin was able to protect the testicular tissue from ischemia-reperfusion damage caused by induced torsion/detorsion.
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http://dx.doi.org/10.1016/j.yexmp.2020.104563DOI Listing
December 2020

Boronic Acid-Functionalized Two-Dimensional MoS at Biointerfaces.

Langmuir 2020 06 10;36(24):6706-6715. Epub 2020 Jun 10.

Department of Chemistry, Faculty of Science, Lorestan University, Khorramabad 0663332145, Iran.

While noncovalent interactions at two-dimensional nanobiointerfaces are extensively investigated, less knowledge about covalent interactions at this interface is available. In this work, boronic acid-functionalized 2D MoS was synthesized and its covalent multivalent interactions with bacteria and nematodes were investigated. Polymerization of glycidol by freshly exfoliated MoS and condensation of 2,5-thiophenediylbisboronic acid on the produced platform resulted in boronic acid-functionalized 2D MoS. The destructive interactions between 2D MoS and bacteria as well as nematodes were significantly amplified by boronic acid functional groups. Because of the high antibacterial and antinematodal activities of boronic acid-functionalized 2D MoS, its therapeutic efficacy for diabetic wound healing was investigated. The infected diabetic wounds were completely healed 10 days after treatment with boronic acid-functionalized 2D MoS, and a normal structure for recovered tissues including different layers of skin, collagen, and blood vessels was detected.
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http://dx.doi.org/10.1021/acs.langmuir.0c00776DOI Listing
June 2020

Molecular characterisation of isolates from Iran in the period 2012-2017.

Acta Vet Hung 2019 09;67(3):347-359

Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj, Iran.

(MG) causes chronic non-fatal diseases in the poultry industry with a remarkable decrease in feed consumption, egg production and other production indices. To study the genetic characteristics of MG isolates in commercial and backyard poultry flocks, 21 positive samples from different regions of Iran were analysed in the period 2012-2017. Typical macroscopic and histopathological lesions of the upper respiratory tract and lungs were found, similar to those reported by other researchers. A 298-361 bp part of the gene was sequenced and analysed. Based on the phylogenetic analysis, the Iranian MG isolates fell into four distinct subgroups. The nucleotide difference between subgroups 1 and 4 was remarkable (91.6-94.9%). A 22-amino-acid insertion was present in two of the studied MG isolates, not observed in other vaccine and standard MG strains. The Iranian Veterinary Organisation (IVO) should consider the observed diversity of prevalent MG isolates from both commercial and backyard flocks in designing the strategy for controlling MG. More studies are needed to understand modifications in MG antigenicity and pathogenicity because of the observed genetic variations.
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http://dx.doi.org/10.1556/004.2019.036DOI Listing
September 2019

Effect of Local Administration of Verapamil Combined with Chitosan Based Hybrid Nanofiber Conduit on Transected Sciatic Nerve in Rat.

Bull Emerg Trauma 2019 Jan;7(1):28-34

Department of Surgery and Diagnostic Imaging, Urmia University, Urmia, Iran.

Objective: To assess the effect of locally administered verapamil on transected peripheral nerve regeneration and functional recovery.

Methods: Sixty male healthy white Wistar rats were divided into four experimental groups (n=15), randomly: In transected group (TC), left sciatic nerve was transected and stumps were fixed in the adjacent muscle. In treatment group defect was bridged using chitosan tube (CHIT/Verapamil) filled with 10 µL verapamil (100ng/mL). In chitosan conduit group (CHIT), the tube was filled with phosphate-buffered saline alone. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. The repair trend was examined based on behavioral and performance tests as well as the variations of the gastrocnemius muscle, morphometric indices, and immunohistochemical indices.

Results: Sciatic nerve functional study, muscle mass and morphometric indices confirmed faster recovery of regenerated axons in CHIT/Verapamil than CHIT group ( = 0.001). When loaded in a chitosan tube verapamil accelerated and improved functional recovery and morphometric indices of sciatic nerve. Immunohistochemical analysis revealed the S-100 protein was vastly present in the transverse nerve sections and the myelin sheath. In the treatment group (chit/verapamil), the immunohistochemical susceptibility of the axons being repaired and the axons in the myelin sheath to S-100 protein was higher than the other groups.

Conclusion: The present study demonstrated that a single local application of verapamil could accelerate functional recovery after transection of sciatic nerve.
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http://dx.doi.org/10.29252/beat-070104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360005PMC
January 2019

Metastatic giant cell osteosarcoma in a cat.

Vet Res Forum 2018 15;9(3):289-292. Epub 2018 Sep 15.

Department of Pathology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

A four-year-old male Persian cat was referred with three weeks history of progressive lameness due to a rigid osseous mass with 3.50×2.50×2.00 cm in dimensions in his left arm. In the histopathological evaluation of bone biopsy, two distinct populations of cells including multinucleated giant cells and oval cells which embedded in a fibro-osseous stroma and surrounded by lamellar bone trabeculae were observed. At necropsy, multiple metastatic nodules with different sizes unveiled in the liver and spleen. Microscopically, those tumor cells which already described in the bone lesion were also infiltrated to the liver and spleen. The neoplastic cells had no immunoreaction to CD68, desmin, alpha smooth muscle actin, S100, CD20, CD3 and pancytokeratin, but only a few cells had reaction to vimentin were similar to fibroblasts.
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http://dx.doi.org/10.30466/vrf.2018.32087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198165PMC
September 2018

Cerebral astrocytoma in a sheep.

Vet Res Forum 2017 15;8(3):269-273. Epub 2017 Sep 15.

Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.

Astrocytoma as one of the most common central nervous system (CNS) tumors is rarely reported in veterinary literature. A 7-year-old Persian Lori-Bakhtiari ewe was presented to the clinic with a two months history of progressive blindness, nystagmus to the right, bilaterally decreased pupillary reflexes, head pressing and paddling. At necropsy, a whitish well-circumscribed mass with dimensions of 3.50×2.50×1.50 cm was observed in the dorsal parietal lobe of the left cerebral hemisphere. Microscopically, the mass was well-circumscribed and highly cellular, consisted of round to elongated cells with scant and vacuolated cytoplasm with few, flaccid processes. The nuclei were round to oval with densely stippled chromatin and indistinct nucleoli. Immunohistochemical analyses showed positive staining for vimentin, S100 and glial fibrillary acidic protein. Definitive diagnosis of cerebral protoplasmic astrocytoma was made on the basis of the histopathological and immunohistochemical findings. This type of neoplasm should be included in the differential diagnosis of CNS lesions in the sheep.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653893PMC
September 2017

Leydig cell tumor in ovary of a German Shepherd bitch: An immunohistopathological study.

Vet Res Forum 2017 15;8(1):81-84. Epub 2017 Mar 15.

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Leydig cell tumor as a sex-cord stromal tumor is a relatively uncommon ovarian tumor in bitch. A 10-year-old female German Shepherd dog was presented because of protrusion of a large tumor-like mass of 16 × 14 × 7 cm in dimensions from her vagina. After stabilization of the patient, the mass was removed surgically and concurrent ovariohysterectomy was also performed. Macroscopically, the healthy tissue of the right ovary was totally replaced by a homogeneous, brown and firm mass. The neoplasm was well-circumscribed and nodular and it was clearly demarcated from the healthy tissue. Histological examination revealed the presence of solid sheets and acinar structures composed of polyhedral to elongated cells. The neoplastic cells had large, eosinophilic, and vacuolated cytoplasms with round to oval nuclei and expressed vimentin on immunohistochemical examination. These gross, microscopic and immuno-histochemical features are characteristics of ovarian Leydig cell tumor.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413316PMC
March 2017
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