Publications by authors named "George Rodriquez"

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Dissociation and fractionation of heavy and light chains from IgG monoclonal antibodies.

J Chromatogr A 2011 Apr 13;1218(17):2402-4. Epub 2010 Dec 13.

Bioprocessing Technology Institute, 20 Biopolis Way, Centros, Singapore.

A basic method for dissociation and fractionation of monoclonal IgG heavy and light chain is described. It employs less noxious and hazardous reagents than the classical mercaptoethanol/propionic acid process and replaces size exclusion chromatography with cation exchange on a monolith to improve productivity. Significant scope remains to refine the conditions. The method can be applied to other disulfide bonded proteins with significant affinity for cation exchangers.
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April 2011